RLG00000030831

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014559
Physical Location & Seq
Reverse (-)
58650 .. 65313
6664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030831

Sequence Viewer

Length: 1674 bp
ATGCTGGTTACGTCCGCCCGAACACTCCTCAGAAGGCGGCTATGCTCTATTGAGTTGTTAAAAGATTATACGTCAAGGAGAAGTGGTATTAGCTGTAGTAGTAGTACCTGGGCGGCAGCTAATGTTGAAGAAGATGCTGCTGCTGCTGCTGCTTCTTGTTCCTCGTCTGAGTCGGTGTCAGTCGCCTCCCCCCCAGTCAGGAATAATATGCTCCGACGACGACGTTTCGGTATTCGGAGGAACAGCGAGAGCCGGAGCACAAAGGATACGGATGCCGTGGAGCTGGCATTGGACTCAGTGGTGAAGATCTTTACTGTGGCGAGCAGCCCTAACTACTTCCTGCCCTGGCAGAACAAGTCCCAACGAGAGACCATGGGTTCCGGATTTGTAATGGCCGGCAAGAAAATTCTTACAAACGCTCATGTAGTTGCTGATCATACATTTGTTCTAGTAAGAAAGCATGGCTCTCCTACCAAGTATAAAGCGCAAGTTGAAGCTGTTGGTCATGAATGTGATTTAGCCATTTTGAGTGTTGAAACTGAAAAGTTCTGGGAGGGTATGAATTCCCTTGAGCTAGGGGATATTCCTTTTCTGCAAGAAGCTGTTGCTGTTGTTGGATATCCTCAAGGTGGGGACAATATCTCTGTTACTAAAGGTGTTGTTTCCAGGGTTGAACCTACACAGTATGTTCATGGTGCAACCCAGCTTATGGCAATACAGATTGATGCAGCAATAAATCCCGGAAATAGTGGTGGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATGTTGGATTCTGCTCTCTAGGTTTGTCATGCCAGCCTACTGAAAATGTTCAACTCCGGAACCACTTTCAAATGCGTCCTGAAATGACTGGGGTACTAGTGAGCAAAATCAACCCTCTTTCGGATGCCCACAAAGTCTTAAAGAAAGATGATGTAGTTCTTTTCTTTGATGGAGTTCCTGTAGCAAATGATGGAACAGTTCACTTCAGGAACAGAGAGCGGATAACATTTGATCATTTGGTGTCCATGAAGAAACCCAATGAAACAGCTGTAGTTAGAGTTTTGAGGGATGGCGAAGAATATGAATTCAGTATCACCCTTCAACCTTTACAACCGTTAGTTCCAGTTCATCAGTTCGATAAACTTCCTAGTTATTACATTTTTGCTGGTTTTGTCTTTGTTCCGCTCACTCAACCTTACCTTCATGAGTATGGAGAAGATTGGTATAATACTTCACCTCGTAAATTATGCGAAATCGCATTGAGGGAACCACCTAAACGAGCTGGTGAACAGATTGTCATCCTTTCTCAGGTACTCATGGATGATATCAATGCTGGATATGAACGTCTTGCAGAGCTGCAGGTCAAGAAGGTTAACGGGATAGAAGTTGAAAATTTGAAACATTTATGCCAACTTGTCGAAAACTGTAGTGAAGAGAGCGTGCGTTTTGATTTGGATGATGATAGAGTTGTTGTGTTGAACTATCATTTAGCAAAAGTTGCGACTTCAAAAATTCTAAATTGCCACAGGATACCCTCTGCAATGTCTAATGATCTTTTAGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

558

Amino Acids

61.7

Weight (kDa)

6.59

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 109 - 287 1.4e-17 Trypsin
Trypsin_2 PF13365 126 - 255 2.1e-20 Trypsin-like peptidase domain
PDZ_2 PF13180 319 - 402 3.8e-06 PDZ domain
PDZ_3 PF17815 409 - 553 1.2e-55 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1456
AccB7I CCANNNNNTGG 1 cut(s) 709
AccBSI CCGCTC 2 cut(s) 1105, 1291
AccIII TCCGGA 2 cut(s) 380, 942
AciI CCGC 5 cut(s) 15, 37, 113, 1105, 1289
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 5 cut(s) 405, 562, 1190, 1498, 1617
AcuI CTGAAG 1 cut(s) 1075
AfaI GTAC 3 cut(s) 106, 981, 1419
AfiI CCNNNNNNNGG 5 cut(s) 198, 629, 709, 1006, 1414
AhlI ACTAGT 1 cut(s) 982
AjnI CCWGG 3 cut(s) 107, 344, 665
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 1 cut(s) 362
Aor13HI TCCGGA 2 cut(s) 380, 942
AoxI GGCC 2 cut(s) 393, 754
ApeKI GCWGC 9 cut(s) 116, 137, 140, 143, 146, 149, 324, 728, 1462
ApoI RAATTY 5 cut(s) 405, 562, 1190, 1498, 1617
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 4 cut(s) 313, 1192, 1332, 1403
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 9 cut(s) 124, 127, 128, 130, 133, 136, 336, 740, 1449
BccI CCATC 3 cut(s) 1049, 1070, 1169
BceAI ACGGC 1 cut(s) 260
BciT130I CCWGG 3 cut(s) 109, 346, 667
BciVI GTATCC 2 cut(s) 259, 1629
BclI TGATCA 2 cut(s) 433, 1117
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 362
BcuI ACTAGT 1 cut(s) 982
BfaI CTAG 5 cut(s) 449, 575, 905, 983, 1254
BfmI CTRYAG 5 cut(s) 94, 1065, 1155, 1463, 1531
BfuAI ACCTGC 1 cut(s) 1456
BfuI GTATCC 2 cut(s) 259, 1629
BglII AGATCT 1 cut(s) 306
Bme1390I CCNGG 4 cut(s) 109, 346, 667, 741
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 3 cut(s) 379, 947, 1374
BmrFI CCNGG 4 cut(s) 109, 346, 667, 741
BmrI ACTGGG 2 cut(s) 188, 984
BmsI GCATC 4 cut(s) 124, 262, 715, 1000
BmuI ACTGGG 2 cut(s) 188, 984
BpmI CTGGAG 1 cut(s) 885
BpuEI CTTGAG 2 cut(s) 590, 609
BpuMI CCSGG 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 1403
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 5 cut(s) 108, 276, 344, 372, 666
BsaWI WCCGGW 2 cut(s) 380, 942
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 1050, 1080
Bsc4I CCNNNNNNNGG 5 cut(s) 198, 629, 709, 1006, 1414
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 3 cut(s) 194, 979, 1229
Bse3DI GCAATG 1 cut(s) 1653
Bse8I GATNNNNATC 1 cut(s) 1403
BseAI TCCGGA 2 cut(s) 380, 942
BseBI CCWGG 3 cut(s) 109, 346, 667
BseDI CCNNGG 5 cut(s) 108, 276, 344, 372, 666
BseGI GGATG 6 cut(s) 277, 1015, 1180, 1404, 1432, 1567
BseJI GATNNNNATC 1 cut(s) 1403
BseLI CCNNNNNNNGG 5 cut(s) 198, 629, 709, 1006, 1414
BseMI GCAATG 1 cut(s) 1653
BseMII CTCAG 4 cut(s) 43, 159, 309, 1427
BseNI ACTGG 3 cut(s) 194, 979, 1229
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 9 cut(s) 124, 127, 128, 130, 133, 136, 336, 740, 1449
BseYI CCCAGC 2 cut(s) 702, 757
BsgI GTGCAG 1 cut(s) 831
BshFI GGCC 2 cut(s) 395, 756
BsiHKAI GWGCWC 1 cut(s) 260
BsiSI CCGG 5 cut(s) 253, 381, 396, 741, 943
BslFI GGGAC 2 cut(s) 343, 647
BslI CCNNNNNNNGG 5 cut(s) 198, 629, 709, 1006, 1414
BsmAI GTCTC 1 cut(s) 362
BsmFI GGGAC 2 cut(s) 343, 647
BsnI GGCC 2 cut(s) 395, 756
Bso31I GGTCTC 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 260
Bsp13I TCCGGA 2 cut(s) 380, 942
Bsp143I GATC 4 cut(s) 306, 433, 1117, 1657
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 5 cut(s) 15, 37, 113, 1105, 1289
BspANI GGCC 2 cut(s) 395, 756
BspCNI CTCAG 4 cut(s) 42, 160, 308, 1426
BspEI TCCGGA 2 cut(s) 380, 942
BspHI TCATGA 2 cut(s) 505, 1309
BspLI GGNNCC 3 cut(s) 379, 947, 1374
BspMAI CTGCAG 1 cut(s) 1467
BspMI ACCTGC 1 cut(s) 1456
BspTNI GGTCTC 1 cut(s) 362
BsrBI CCGCTC 2 cut(s) 1105, 1291
BsrDI GCAATG 1 cut(s) 1653
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 3 cut(s) 194, 979, 1229
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 5 cut(s) 108, 276, 344, 372, 666
BssMI GATC 4 cut(s) 306, 433, 1117, 1657
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 109, 346, 667
Bst4CI ACNGT 5 cut(s) 316, 684, 1084, 1221, 1532
Bst6I CTCTTC 1 cut(s) 1533
BstAPI GCANNNNNTGC 1 cut(s) 1604
BstC8I GCNNGC 5 cut(s) 285, 322, 397, 920, 1547
BstDEI CTNAG 4 cut(s) 29, 168, 295, 1413
BstDSI CCRYGG 2 cut(s) 276, 372
BstENI CCTNNNNNAGG 1 cut(s) 1412
BstF5I GGATG 6 cut(s) 277, 1015, 1180, 1404, 1432, 1567
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 4 cut(s) 309, 436, 1120, 1660
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 4 cut(s) 306, 433, 1117, 1657
BstMWI GCNNNNNNNGC 5 cut(s) 143, 146, 149, 788, 1604
BstNI CCWGG 3 cut(s) 109, 346, 667
BstSCI CCNGG 4 cut(s) 107, 344, 665, 739
BstSFI CTRYAG 5 cut(s) 94, 1065, 1155, 1463, 1531
BstV1I GCAGC 9 cut(s) 124, 127, 128, 130, 133, 136, 336, 740, 1449
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BsuI GTATCC 2 cut(s) 259, 1629
BsuRI GGCC 2 cut(s) 395, 756
BtgI CCRYGG 2 cut(s) 276, 372
BtsCI GGATG 6 cut(s) 277, 1015, 1180, 1404, 1432, 1567
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 1456
Cac8I GCNNGC 5 cut(s) 285, 322, 397, 920, 1547
CciI TCATGA 2 cut(s) 505, 1309
CfoI GCGC 1 cut(s) 487
Cfr10I RCCGGY 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 755
CseI GACGC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 105, 980, 1418
CviQI GTAC 3 cut(s) 105, 980, 1418
DdeI CTNAG 4 cut(s) 29, 168, 295, 1413
DpnI GATC 4 cut(s) 308, 435, 1119, 1659
DpnII GATC 4 cut(s) 306, 433, 1117, 1657
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 1533
EarI CTCTTC 1 cut(s) 1533
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 372
Eco31I GGTCTC 1 cut(s) 362
Eco32I GATATC 2 cut(s) 620, 1432
Eco57I CTGAAG 1 cut(s) 1075
EcoNI CCTNNNNNAGG 1 cut(s) 1412
EcoRI GAATTC 2 cut(s) 562, 1190
EcoRII CCWGG 3 cut(s) 107, 344, 665
EcoRV GATATC 2 cut(s) 620, 1432
EcoT14I CCWWGG 1 cut(s) 372
ErhI CCWWGG 1 cut(s) 372
FaqI GGGAC 2 cut(s) 343, 647
FbaI TGATCA 2 cut(s) 433, 1117
FokI GGATG 6 cut(s) 284, 1022, 1187, 1391, 1439, 1574
FspBI CTAG 5 cut(s) 449, 575, 905, 983, 1254
GlaI GCGC 1 cut(s) 486
GsaI CCCAGC 2 cut(s) 706, 761
GsuI CTGGAG 1 cut(s) 885
HaeIII GGCC 2 cut(s) 395, 756
HapII CCGG 5 cut(s) 253, 381, 396, 741, 943
HgaI GACGC 1 cut(s) 950
HhaI GCGC 1 cut(s) 487
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
HinfI GANTC 3 cut(s) 170, 293, 894
HpaI GTTAAC 1 cut(s) 1480
HpaII CCGG 5 cut(s) 253, 381, 396, 741, 943
HphI GGTGA 4 cut(s) 313, 1192, 1332, 1403
Hpy166II GTNNAC 3 cut(s) 1087, 1394, 1480
Hpy188I TCNGA 5 cut(s) 32, 169, 215, 237, 1009
Hpy188III TCNNGA 8 cut(s) 199, 381, 506, 943, 965, 1093, 1310, 1471
Hpy8I GTNNAC 3 cut(s) 1087, 1394, 1480
Hpy99I CGWCG 3 cut(s) 219, 222, 225
HpyAV CCTTC 4 cut(s) 27, 1214, 1316, 1468
HpyCH4III ACNGT 5 cut(s) 316, 684, 1084, 1221, 1532
HpyCH4IV ACGT 4 cut(s) 11, 71, 223, 1450
HpyCH4V TGCA 7 cut(s) 595, 698, 728, 812, 1457, 1465, 1646
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 146, 149, 788, 1604
HpyF3I CTNAG 4 cut(s) 29, 168, 295, 1413
HpySE526I ACGT 4 cut(s) 11, 71, 223, 1450
HspAI GCGC 1 cut(s) 485
Kpn2I TCCGGA 2 cut(s) 380, 942
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
Ksp22I TGATCA 2 cut(s) 433, 1117
KspAI GTTAAC 1 cut(s) 1480
Kzo9I GATC 4 cut(s) 306, 433, 1117, 1657
LmnI GCTCC 3 cut(s) 216, 255, 280
Lsp1109I GCAGC 9 cut(s) 124, 127, 128, 130, 133, 136, 336, 740, 1449
LweI GCATC 4 cut(s) 124, 262, 715, 1000
MaeI CTAG 5 cut(s) 449, 575, 905, 983, 1254
MaeII ACGT 4 cut(s) 11, 71, 223, 1450
MaeIII GTNAC 2 cut(s) 7, 646
MalI GATC 4 cut(s) 308, 435, 1119, 1659
MbiI CCGCTC 2 cut(s) 1105, 1291
MboI GATC 4 cut(s) 306, 433, 1117, 1657
MboII GAAGA 8 cut(s) 140, 143, 316, 884, 1147, 1193, 1334, 1550
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 8 cut(s) 405, 562, 831, 1190, 1349, 1498, 1617, 1624
MlyI GAGTC 2 cut(s) 179, 287
MmeI TCCRAC 3 cut(s) 238, 595, 871
MroI TCCGGA 2 cut(s) 380, 942
MroNI GCCGGC 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 3 cut(s) 59, 1025, 1479
MslI CAYNNNNRTG 2 cut(s) 510, 1314
MspA1I CMGCKG 1 cut(s) 1154
MspI CCGG 5 cut(s) 253, 381, 396, 741, 943
MspR9I CCNGG 4 cut(s) 109, 346, 667, 741
MvaI CCWGG 3 cut(s) 109, 346, 667
MwoI GCNNNNNNNGC 5 cut(s) 143, 146, 149, 788, 1604
NaeI GCCGGC 1 cut(s) 397
NciI CCSGG 1 cut(s) 741
NcoI CCATGG 1 cut(s) 372
NdeII GATC 4 cut(s) 306, 433, 1117, 1657
NgoMIV GCCGGC 1 cut(s) 395
NlaIV GGNNCC 3 cut(s) 379, 947, 1374
PagI TCATGA 2 cut(s) 505, 1309
PdiI GCCGGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 1 cut(s) 894
PflMI CCANNNNNTGG 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 739
PleI GAGTC 2 cut(s) 178, 287
PpsI GAGTC 2 cut(s) 178, 287
Psp6I CCWGG 3 cut(s) 107, 344, 665
PspFI CCCAGC 2 cut(s) 702, 757
PspGI CCWGG 3 cut(s) 107, 344, 665
PspN4I GGNNCC 3 cut(s) 379, 947, 1374
PspPI GGNCC 1 cut(s) 755
PstI CTGCAG 1 cut(s) 1467
PsuI RGATCY 1 cut(s) 306
PvuII CAGCTG 1 cut(s) 1154
RsaI GTAC 3 cut(s) 106, 981, 1419
RsaNI GTAC 3 cut(s) 105, 980, 1418
RseI CAYNNNNRTG 2 cut(s) 510, 1314
SaqAI TTAA 3 cut(s) 59, 1025, 1479
Sau3AI GATC 4 cut(s) 306, 433, 1117, 1657
Sau96I GGNCC 1 cut(s) 755
SchI GAGTC 2 cut(s) 179, 287
ScrFI CCNGG 4 cut(s) 109, 346, 667, 741
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 4 cut(s) 124, 262, 715, 1000
SfcI CTRYAG 5 cut(s) 94, 1065, 1155, 1463, 1531
SmiMI CAYNNNNRTG 2 cut(s) 510, 1314
SmlI CTYRAG 2 cut(s) 569, 624
SmoI CTYRAG 2 cut(s) 569, 624
SpeI ACTAGT 1 cut(s) 982
Sse9I AATT 8 cut(s) 405, 562, 831, 1190, 1349, 1498, 1617, 1624
SsiI CCGC 5 cut(s) 15, 37, 113, 1105, 1289
SspI AATATT 1 cut(s) 820
SspMI CTAG 5 cut(s) 449, 575, 905, 983, 1254
StyD4I CCNGG 4 cut(s) 107, 344, 665, 739
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 5 cut(s) 316, 684, 1084, 1221, 1532
TaiI ACGT 4 cut(s) 14, 74, 226, 1453
TaqI TCGA 2 cut(s) 1242, 1524
TasI AATT 8 cut(s) 405, 562, 831, 1190, 1349, 1498, 1617, 1624
TauI GCSGC 2 cut(s) 40, 116
TfiI GAWTC 1 cut(s) 894
Tru1I TTAA 3 cut(s) 59, 1025, 1479
Tru9I TTAA 3 cut(s) 59, 1025, 1479
TscAI CASTG 1 cut(s) 303
TseI GCWGC 9 cut(s) 116, 137, 140, 143, 146, 149, 324, 728, 1462
TspDTI ATGAA 9 cut(s) 522, 575, 680, 1148, 1161, 1203, 1223, 1298, 1461
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 303
Van91I CCANNNNNTGG 1 cut(s) 709
XagI CCTNNNNNAGG 1 cut(s) 1412
XapI RAATTY 5 cut(s) 405, 562, 1190, 1498, 1617
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 5 cut(s) 449, 575, 905, 983, 1254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.