Rh5DG000100

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014559
Physical Location & Seq
Reverse (-)
17847 .. 24478
6632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG000100.1

Sequence Viewer

Length: 1671 bp
ATGCTGGTTACGTCCGCCCGAACACTCCTCAGAAGGCGGCTATGCTCTATTGAGTTGTTAAAAGATTATACATCAAGGAGAAGTGGTATTAGCTGTAGTAGCAGTACCTGGGCGGCAGCTAATGTTGAAGAAGATGCTGCTGCTGCTGCTTCTTGTTCCTCGTCTGAGTCGGTGTCGGTCGCCTCCCCCTCAGTCAGGAATAATATGCTCCGACGACGACGTTTCGGTATTCGGAGGAACAACGAGAGCCGGAGCACAAAGGATACGGATGCCGTAGAGCTGGCATTGGACTCAGTGGTGAAGATCTTTACTGTGGCGAGCAGCCCTAACTACTTCCTGCCCTGGCAGAACAAGTCCCAACGAGAGACCATGGGTTCCGGATTTGTAATCGCCGGCAAGAAAATTCTTACAAACGCTCATGTAGTTGCTGATCATACATTTGTTCTAGTAAGAAAGCATGGCTCTCCTACCAAGTATAAAGCGCAAGTTGAAGCTGTTGGTCATGAATGTGATTTAGCTATTTTGAGTGTTGAAACTGAAAAGTTCTGGGAGGGTATGAATTCCCTTGAGCTAGGGGATATTCCTTTTCTGCAAGAAGCTGTTGCTGTTGTTGGATATCCTCAAGGTGGGGACAATATCTCTGTTACTAAAGGTGTTGTTTCCAGGGTTGAACCTACACAATATGTTCATGGTGCAACCCAGCTTATGGCAATACAGATTGATGCAGCAATAAATCCCGGAAATAGTGGTGGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATGTGTTGGATTCTGCTCTCTAGGTTTGTCATGCCAGCCTACTGAAAATGTTCAACTCCGGAACCACTTTCAAATGCGTCCTGAAATGACTGGGGTACTAGTGAGCAAAATCAACCCTCTTTCGGATGCCCACAAAGTCTTAAAGAAAGATGATGTTGTTCTTTTCTTTGATGGAGTTCCTGTAGCAAATGATGGAACAGTTCACTTCAGGAACAGAGAGCGGATAACATTTGATCATTTGGTGTCCATGAAGAAACCCAATGAAACAGCTGTAGTTAGAGTTTTGAGGGATGGCGAAGAATATGAATTCAGTATCACCCTTCAACCTTTACAACCGTTAGTTCCAGTTCATCAGTTCGATAAACTTCCTAGTTATTACATTTTTGCTGGTTTGGTCTTTGTTCCGCTCACTCAACCTTACCTTCATGAGTATGGAGAAGATTGGTATAATACTTCACCTCGTAAATTATGCGAAATCGCATTGAGGGAACCACCTAAAAGAGCTGGTGAACAGATTGTCATCCTTTCTCAGGTACTCATGGATGATATCAATGCTGGATATGAACGTCTTGCAGAGCTGCAGGTTAAGAAGGTAAACAGGATAGAAGTTGAAAATTTGAAACATTTATGCCAACTTGTCGAAAACTGTAGTGAAGAGAGCGTGCGTTTTGATTTGGATGATGATAGAGTTGTTGTGTTGAACTATCATTTAGCAAAAGTTGCGACTTCAAAAATTCTAAATTGCCACAGGATACCCTCTGCAATGTCTAATGATCTTTTAGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

556

Amino Acids

61.63

Weight (kDa)

6.75

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 108 - 286 3.9e-18 Trypsin
Trypsin_2 PF13365 125 - 254 6e-21 Trypsin-like peptidase domain
PDZ_2 PF13180 318 - 401 3.7e-06 PDZ domain
PDZ_3 PF17815 408 - 552 5.6e-55 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1453
AccB7I CCANNNNNTGG 1 cut(s) 706
AccBSI CCGCTC 2 cut(s) 1102, 1288
AccIII TCCGGA 2 cut(s) 377, 939
AciI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
AcsI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
AcuI CTGAAG 1 cut(s) 1072
AfaI GTAC 3 cut(s) 106, 978, 1416
AfiI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
AhlI ACTAGT 1 cut(s) 979
AjnI CCWGG 3 cut(s) 107, 341, 662
Alw21I GWGCWC 1 cut(s) 257
Alw26I GTCTC 1 cut(s) 359
AlwNI CAGNNNCTG 1 cut(s) 108
Aor13HI TCCGGA 2 cut(s) 377, 939
AoxI GGCC 1 cut(s) 751
ApeKI GCWGC 8 cut(s) 116, 137, 140, 143, 146, 321, 725, 1459
ApoI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 1 cut(s) 752
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 4 cut(s) 310, 1189, 1329, 1400
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BccI CCATC 3 cut(s) 1046, 1067, 1166
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 3 cut(s) 109, 343, 664
BciVI GTATCC 2 cut(s) 256, 1626
BclI TGATCA 2 cut(s) 430, 1114
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 1 cut(s) 359
BcuI ACTAGT 1 cut(s) 979
BfaI CTAG 5 cut(s) 446, 572, 902, 980, 1251
BfmI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
BfuAI ACCTGC 1 cut(s) 1453
BfuI GTATCC 2 cut(s) 256, 1626
BglII AGATCT 1 cut(s) 303
Bme1390I CCNGG 4 cut(s) 109, 343, 664, 738
BmgT120I GGNCC 1 cut(s) 752
BmiI GGNNCC 3 cut(s) 376, 944, 1371
BmrFI CCNGG 4 cut(s) 109, 343, 664, 738
BmrI ACTGGG 1 cut(s) 981
BmsI GCATC 4 cut(s) 124, 259, 712, 997
BmuI ACTGGG 1 cut(s) 981
BpmI CTGGAG 1 cut(s) 882
BpuEI CTTGAG 2 cut(s) 587, 606
BpuMI CCSGG 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 1400
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 4 cut(s) 108, 341, 369, 663
BsaWI WCCGGW 2 cut(s) 377, 939
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 1047, 1077
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 2 cut(s) 976, 1226
Bse3DI GCAATG 1 cut(s) 1650
Bse8I GATNNNNATC 1 cut(s) 1400
BseAI TCCGGA 2 cut(s) 377, 939
BseBI CCWGG 3 cut(s) 109, 343, 664
BseDI CCNNGG 4 cut(s) 108, 341, 369, 663
BseGI GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BseJI GATNNNNATC 1 cut(s) 1400
BseLI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
BseMI GCAATG 1 cut(s) 1650
BseMII CTCAG 5 cut(s) 43, 156, 204, 306, 1424
BseNI ACTGG 2 cut(s) 976, 1226
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BseYI CCCAGC 2 cut(s) 699, 754
BsgI GTGCAG 1 cut(s) 828
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 1 cut(s) 753
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 257
BsiSI CCGG 5 cut(s) 250, 378, 393, 738, 940
BslFI GGGAC 2 cut(s) 340, 644
BslI CCNNNNNNNGG 5 cut(s) 195, 626, 706, 1003, 1411
BsmAI GTCTC 1 cut(s) 359
BsmFI GGGAC 2 cut(s) 340, 644
BsnI GGCC 1 cut(s) 753
Bso31I GGTCTC 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 257
Bsp13I TCCGGA 2 cut(s) 377, 939
Bsp143I GATC 4 cut(s) 303, 430, 1114, 1654
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
BspANI GGCC 1 cut(s) 753
BspCNI CTCAG 5 cut(s) 42, 157, 203, 305, 1423
BspEI TCCGGA 2 cut(s) 377, 939
BspHI TCATGA 2 cut(s) 502, 1306
BspLI GGNNCC 3 cut(s) 376, 944, 1371
BspMAI CTGCAG 1 cut(s) 1464
BspMI ACCTGC 1 cut(s) 1453
BspTNI GGTCTC 1 cut(s) 359
BsrBI CCGCTC 2 cut(s) 1102, 1288
BsrDI GCAATG 1 cut(s) 1650
BsrFI RCCGGY 1 cut(s) 392
BsrI ACTGG 2 cut(s) 976, 1226
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 4 cut(s) 108, 341, 369, 663
BssMI GATC 4 cut(s) 303, 430, 1114, 1654
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 3 cut(s) 109, 343, 664
Bst4CI ACNGT 4 cut(s) 313, 1081, 1218, 1529
Bst6I CTCTTC 1 cut(s) 1530
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstC8I GCNNGC 5 cut(s) 282, 319, 394, 917, 1544
BstDEI CTNAG 5 cut(s) 29, 165, 190, 292, 1410
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 2 cut(s) 193, 1409
BstF5I GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 4 cut(s) 306, 433, 1117, 1657
BstMAI GTCTC 1 cut(s) 359
BstMBI GATC 4 cut(s) 303, 430, 1114, 1654
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 5 cut(s) 99, 143, 146, 785, 1601
BstNI CCWGG 3 cut(s) 109, 343, 664
BstSCI CCNGG 4 cut(s) 107, 341, 662, 736
BstSFI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
BstV1I GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuI GTATCC 2 cut(s) 256, 1626
BsuRI GGCC 1 cut(s) 753
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 6 cut(s) 274, 1012, 1177, 1401, 1429, 1564
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 1453
Cac8I GCNNGC 5 cut(s) 282, 319, 394, 917, 1544
CaiI CAGNNNCTG 1 cut(s) 108
CciI TCATGA 2 cut(s) 502, 1306
CfoI GCGC 1 cut(s) 484
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 752
CseI GACGC 1 cut(s) 947
Csp6I GTAC 3 cut(s) 105, 977, 1415
CviQI GTAC 3 cut(s) 105, 977, 1415
DdeI CTNAG 5 cut(s) 29, 165, 190, 292, 1410
DpnI GATC 4 cut(s) 305, 432, 1116, 1656
DpnII GATC 4 cut(s) 303, 430, 1114, 1654
Eam1104I CTCTTC 1 cut(s) 1530
EarI CTCTTC 1 cut(s) 1530
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 359
Eco32I GATATC 2 cut(s) 617, 1429
Eco57I CTGAAG 1 cut(s) 1072
EcoNI CCTNNNNNAGG 2 cut(s) 193, 1409
EcoRI GAATTC 2 cut(s) 559, 1187
EcoRII CCWGG 3 cut(s) 107, 341, 662
EcoRV GATATC 2 cut(s) 617, 1429
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaqI GGGAC 2 cut(s) 340, 644
FbaI TGATCA 2 cut(s) 430, 1114
FokI GGATG 6 cut(s) 281, 1019, 1184, 1388, 1436, 1571
FspBI CTAG 5 cut(s) 446, 572, 902, 980, 1251
GlaI GCGC 1 cut(s) 483
GsaI CCCAGC 2 cut(s) 703, 758
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 1 cut(s) 753
HapII CCGG 5 cut(s) 250, 378, 393, 738, 940
HgaI GACGC 1 cut(s) 947
HhaI GCGC 1 cut(s) 484
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HinfI GANTC 3 cut(s) 167, 290, 891
HpaII CCGG 5 cut(s) 250, 378, 393, 738, 940
HphI GGTGA 4 cut(s) 310, 1189, 1329, 1400
Hpy166II GTNNAC 3 cut(s) 1084, 1391, 1477
Hpy188I TCNGA 5 cut(s) 32, 166, 212, 234, 1006
Hpy188III TCNNGA 7 cut(s) 196, 378, 503, 940, 962, 1090, 1307
Hpy8I GTNNAC 3 cut(s) 1084, 1391, 1477
Hpy99I CGWCG 3 cut(s) 216, 219, 222
HpyAV CCTTC 4 cut(s) 27, 1211, 1313, 1465
HpyCH4III ACNGT 4 cut(s) 313, 1081, 1218, 1529
HpyCH4IV ACGT 3 cut(s) 11, 220, 1447
HpyCH4V TGCA 7 cut(s) 592, 695, 725, 809, 1454, 1462, 1643
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 143, 146, 785, 1601
HpyF3I CTNAG 5 cut(s) 29, 165, 190, 292, 1410
HpySE526I ACGT 3 cut(s) 11, 220, 1447
HspAI GCGC 1 cut(s) 482
Kpn2I TCCGGA 2 cut(s) 377, 939
KroI GCCGGC 1 cut(s) 392
KroNI GCCGGC 1 cut(s) 394
Ksp22I TGATCA 2 cut(s) 430, 1114
Kzo9I GATC 4 cut(s) 303, 430, 1114, 1654
LmnI GCTCC 2 cut(s) 213, 252
Lsp1109I GCAGC 8 cut(s) 124, 127, 128, 130, 133, 333, 737, 1446
LweI GCATC 4 cut(s) 124, 259, 712, 997
MaeI CTAG 5 cut(s) 446, 572, 902, 980, 1251
MaeII ACGT 3 cut(s) 11, 220, 1447
MaeIII GTNAC 2 cut(s) 7, 643
MalI GATC 4 cut(s) 305, 432, 1116, 1656
MbiI CCGCTC 2 cut(s) 1102, 1288
MboI GATC 4 cut(s) 303, 430, 1114, 1654
MboII GAAGA 8 cut(s) 140, 143, 313, 881, 1144, 1190, 1331, 1547
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
MlyI GAGTC 2 cut(s) 176, 284
MmeI TCCRAC 3 cut(s) 235, 592, 868
MroI TCCGGA 2 cut(s) 377, 939
MroNI GCCGGC 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 3 cut(s) 59, 1022, 1467
MslI CAYNNNNRTG 2 cut(s) 507, 1311
MspA1I CMGCKG 1 cut(s) 1151
MspI CCGG 5 cut(s) 250, 378, 393, 738, 940
MspR9I CCNGG 4 cut(s) 109, 343, 664, 738
MvaI CCWGG 3 cut(s) 109, 343, 664
MwoI GCNNNNNNNGC 5 cut(s) 99, 143, 146, 785, 1601
NaeI GCCGGC 1 cut(s) 394
NciI CCSGG 1 cut(s) 738
NcoI CCATGG 1 cut(s) 369
NdeII GATC 4 cut(s) 303, 430, 1114, 1654
NgoMIV GCCGGC 1 cut(s) 392
NlaIV GGNNCC 3 cut(s) 376, 944, 1371
PagI TCATGA 2 cut(s) 502, 1306
PdiI GCCGGC 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 1 cut(s) 891
PflMI CCANNNNNTGG 1 cut(s) 706
PfoI TCCNGGA 1 cut(s) 736
PleI GAGTC 2 cut(s) 175, 284
PpsI GAGTC 2 cut(s) 175, 284
Psp6I CCWGG 3 cut(s) 107, 341, 662
PspFI CCCAGC 2 cut(s) 699, 754
PspGI CCWGG 3 cut(s) 107, 341, 662
PspN4I GGNNCC 3 cut(s) 376, 944, 1371
PspPI GGNCC 1 cut(s) 752
PstI CTGCAG 1 cut(s) 1464
PstNI CAGNNNCTG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 303
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 3 cut(s) 106, 978, 1416
RsaNI GTAC 3 cut(s) 105, 977, 1415
RseI CAYNNNNRTG 2 cut(s) 507, 1311
SaqAI TTAA 3 cut(s) 59, 1022, 1467
Sau3AI GATC 4 cut(s) 303, 430, 1114, 1654
Sau96I GGNCC 1 cut(s) 752
SchI GAGTC 2 cut(s) 176, 284
ScrFI CCNGG 4 cut(s) 109, 343, 664, 738
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 4 cut(s) 124, 259, 712, 997
SfcI CTRYAG 5 cut(s) 94, 1062, 1152, 1460, 1528
SmiMI CAYNNNNRTG 2 cut(s) 507, 1311
SmlI CTYRAG 2 cut(s) 566, 621
SmoI CTYRAG 2 cut(s) 566, 621
SpeI ACTAGT 1 cut(s) 979
Sse9I AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
SsiI CCGC 5 cut(s) 15, 37, 113, 1102, 1286
SspI AATATT 1 cut(s) 817
SspMI CTAG 5 cut(s) 446, 572, 902, 980, 1251
StyD4I CCNGG 4 cut(s) 107, 341, 662, 736
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 313, 1081, 1218, 1529
TaiI ACGT 3 cut(s) 14, 223, 1450
TaqI TCGA 2 cut(s) 1239, 1521
TaqII GACCGA 1 cut(s) 166
TasI AATT 8 cut(s) 402, 559, 828, 1187, 1346, 1495, 1614, 1621
TauI GCSGC 2 cut(s) 40, 116
TfiI GAWTC 1 cut(s) 891
Tru1I TTAA 3 cut(s) 59, 1022, 1467
Tru9I TTAA 3 cut(s) 59, 1022, 1467
TscAI CASTG 1 cut(s) 300
TseI GCWGC 8 cut(s) 116, 137, 140, 143, 146, 321, 725, 1459
TspDTI ATGAA 9 cut(s) 519, 572, 677, 1145, 1158, 1200, 1220, 1295, 1458
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 1 cut(s) 300
Van91I CCANNNNNTGG 1 cut(s) 706
XagI CCTNNNNNAGG 2 cut(s) 193, 1409
XapI RAATTY 5 cut(s) 402, 559, 1187, 1495, 1614
XcmI CCANNNNNNNNNTGG 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 5 cut(s) 446, 572, 902, 980, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.