Rroxscaffold_2G00109790

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014559
Physical Location & Seq
Reverse (-)
34000240 .. 34001258
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109790.1

Sequence Viewer

Length: 387 bp
ATGAATTCGTATCACCCTTCAACCTTTACAACCGTTAGTTCCAGTTCATCGTTCGATAAACTTCCTAGTTATTACATCTTTGCTGGTTTGGCCTTTGTTCCGCTCACTCAACCTTACCTTCATGAGTATGGAGAAGATTGGTATAATACTTCACCTCGTAAATTATGCGAAATCGCATTGAGTGAACCACCTAAAAGAGCTGGTGAACAGATTGTCATCCTTCCTCGTGTACTCATGGATGATATCAATGCTGGATATGAACGTCTTGCGCAGAGCCGCAGCCGCATGTTGGGGATCGGAGCTGGATGGCGAGTGACCACCGAGCTTGGTCATGGTGATCAATGGATTGACTTGGCGGACGATGGTGAATCGTTGATTGGGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

128

Amino Acids

14.3

Weight (kDa)

4.88

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDZ_3 PF17815 16 - 92 1.1e-26 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 103
AciI CCGC 4 cut(s) 101, 277, 283, 356
AclWI GGATC 1 cut(s) 302
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 289
AgsI TTSAA 1 cut(s) 21
AjuI GAANNNNNNNTTGG 1 cut(s) 360
AluBI AGCT 3 cut(s) 200, 302, 325
AluI AGCT 3 cut(s) 200, 302, 325
AlwI GGATC 1 cut(s) 302
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 279
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 271
AsuHPI GGTGA 5 cut(s) 5, 144, 215, 347, 377
BauI CACGAG 1 cut(s) 225
BbvI GCAGC 1 cut(s) 291
BccI CCATC 2 cut(s) 300, 356
BclI TGATCA 1 cut(s) 337
BfaI CTAG 1 cut(s) 66
BisI GCNGC 3 cut(s) 277, 280, 283
BlsI GCNGC 3 cut(s) 278, 281, 284
BsaBI GATNNNNATC 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 42
Bse8I GATNNNNATC 1 cut(s) 215
BseGI GGATG 3 cut(s) 216, 244, 311
BseJI GATNNNNATC 1 cut(s) 215
BseLI CCNNNNNNNGG 1 cut(s) 289
BseNI ACTGG 1 cut(s) 42
BseXI GCAGC 1 cut(s) 291
BshFI GGCC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 289
BsnI GGCC 1 cut(s) 92
Bsp143I GATC 2 cut(s) 294, 337
BspACI CCGC 4 cut(s) 101, 277, 283, 356
BspANI GGCC 1 cut(s) 92
BspHI TCATGA 1 cut(s) 121
BspPI GGATC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 42
BssMI GATC 2 cut(s) 294, 337
BssSI CACGAG 1 cut(s) 225
Bst2BI CACGAG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 34
BstF5I GGATG 3 cut(s) 216, 244, 311
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 2 cut(s) 297, 340
BstMBI GATC 2 cut(s) 294, 337
BstMWI GCNNNNNNNGC 2 cut(s) 89, 282
BstNSI RCATGY 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 291
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 3 cut(s) 216, 244, 311
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 230
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 4 cut(s) 122, 235, 286, 332
CviJI RGCY 7 cut(s) 92, 200, 276, 282, 302, 325, 382
CviKI_1 RGCY 7 cut(s) 92, 200, 276, 282, 302, 325, 382
CviQI GTAC 1 cut(s) 230
DpnI GATC 2 cut(s) 296, 339
DpnII GATC 2 cut(s) 294, 337
EciI GGCGGA 1 cut(s) 371
Eco32I GATATC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 4
EcoRV GATATC 1 cut(s) 244
FaeI CATG 4 cut(s) 125, 238, 289, 335
FaiI YATR 8 cut(s) 123, 129, 144, 166, 236, 258, 287, 333
FatI CATG 4 cut(s) 121, 234, 285, 331
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 3 cut(s) 277, 280, 283
FokI GGATG 3 cut(s) 203, 251, 318
Fsp4HI GCNGC 3 cut(s) 277, 280, 283
FspBI CTAG 1 cut(s) 66
FspI TGCGCA 1 cut(s) 270
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 277, 280, 283
HaeIII GGCC 1 cut(s) 92
HhaI GCGC 1 cut(s) 271
Hin1II CATG 4 cut(s) 125, 238, 289, 335
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HinfI GANTC 1 cut(s) 368
HphI GGTGA 5 cut(s) 5, 144, 215, 347, 377
Hpy166II GTNNAC 3 cut(s) 185, 206, 230
Hpy188I TCNGA 1 cut(s) 299
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 3 cut(s) 185, 206, 230
HpyAV CCTTC 3 cut(s) 27, 128, 230
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 282
HpySE526I ACGT 1 cut(s) 262
Hsp92II CATG 4 cut(s) 125, 238, 289, 335
HspAI GCGC 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 2 cut(s) 294, 337
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 5 cut(s) 55, 69, 186, 237, 288
Lsp1109I GCAGC 1 cut(s) 291
MaeI CTAG 1 cut(s) 66
MaeII ACGT 1 cut(s) 262
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 296, 339
MbiI CCGCTC 1 cut(s) 103
MboI GATC 2 cut(s) 294, 337
MboII GAAGA 1 cut(s) 146
MluCI AATT 2 cut(s) 4, 161
MnlI CCTC 2 cut(s) 165, 234
MslI CAYNNNNRTG 1 cut(s) 126
MwoI GCNNNNNNNGC 2 cut(s) 89, 282
NdeII GATC 2 cut(s) 294, 337
NlaIII CATG 4 cut(s) 125, 238, 289, 335
NmuCI GTSAC 1 cut(s) 313
NsbI TGCGCA 1 cut(s) 270
NspI RCATGY 1 cut(s) 289
PagI TCATGA 1 cut(s) 121
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 3 cut(s) 278, 281, 284
RsaI GTAC 1 cut(s) 231
RsaNI GTAC 1 cut(s) 230
RseI CAYNNNNRTG 1 cut(s) 126
SatI GCNGC 3 cut(s) 277, 280, 283
Sau3AI GATC 2 cut(s) 294, 337
SetI ASST 9 cut(s) 26, 115, 120, 157, 193, 202, 265, 304, 327
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 2 cut(s) 4, 161
SsiI CCGC 4 cut(s) 101, 277, 283, 356
SspMI CTAG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 265
TaqI TCGA 1 cut(s) 54
TasI AATT 2 cut(s) 4, 161
TatI WGTACW 1 cut(s) 229
TauI GCSGC 2 cut(s) 279, 285
TfiI GAWTC 1 cut(s) 368
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 1 cut(s) 279
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 4 cut(s) 17, 36, 110, 273
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.