MD08G1000100.v1.1

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014559
Physical Location & Seq
Reverse (-)
8182 .. 13565
5384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1000100.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGCTGGGAACGTCCGTCCATAGACTCACCAGGCTCTCTGTTTCCTCTATAAAGTTAATAGCTAGTAAGGCAACCGCAACCGCAAGAGGGACTAGTATTTGTACTTCTACCAGTACCAGGAGAGCCGCAGCTACTGTTGAAGAAGTTGCGGCCGCCGCCGCTGCGTCTTCTTCTTGTTCCTCCGAGCCAGTTAACTCTAATGGGTCTTTCCCCTACCCCTCATCAGCTGCAGCGCACGTGCAACGACGACGCCCACGACCACGTTTAGGTCTAGGTGATGGGAGGAACATCCAGAGGAGGACCACGACGGATACTGATGCAATAGAGCTAGCATTGGATTCAGTGGTGAAGATCTTCACTGTGACGAGCAGCCCTAACTACTTCCTTCCTTGGCAGAACAAGTCCCAGCGAGAAACCATGGGCTCCGGGTTCGTAATCCGTGGGAAGAAAATTCTGACAAATGCTCATGTAGTGGCTGATCATACCTTTGTACTTGTAAGAAAGCATGGCTCTCCTACCAAGTACAGAGCCCAAGTTAACGCTGTTGGTCATGAATGTGATTTAGCCATTTTGACCGTTGAAAGTCAAGACTTCTGGGAGGGTATGGCTTCCCTCGATCTAGGGGACATTCCTTTTCTGCAACAAGCCGTGGCTGTTGTTGGATATCCTCAAGGTGGTGATAACATTTCAGTTACCAAAGGTGTTGTTTCTAGGGTTGAACCCACTCAATATGTACACGGGGCAACCCAGCTCATGGCAATACAAATTGATGCTGCAATAAATCCAGGGAATAGTGGCGGTCCAGCAATCATGGGCAATAAAGTTGCTGGTGTGGCTTTCCAGAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTCCCAGTAATAAAGCATTTTATTGCTGGAGTGGAAGAAAGTGGCAAGTATGTTGGATTCTGCTCTCTGGGTTTGTCATGCCAACCCATTGAAAATGTTCAACTGAGGAACCACTTTCGGATGCGCCCTGAAATGACCGGGGTACTAGTGAACAAAATTAACCCACTCTCGGATGCCTATAAAGTCTTGAAGAAAGATGATATTATCCTTGTTTTTGATGGAGTGCCTGTAGCGAATGATGGAACAGTTCATTTTCGAAACAGAGAGCGGATAACATTTGACCATTTGGTTTCTATGAAGAAACCCAACGAAACAGCCATAGTTAGAGTTCTGAGGGACGGTGAAGAGTATGAATTCAGTATCGCCCTTCAACCTTTACAGCCATTAGTTCCAGTTCATCAGTTCGATAAGCTTCCTAGTTATTACATTTTTGCTGGTTTGGTCTTTGTTCCACTCACTCAGCCATACCTTCACGAGTATGGAGAAGACTGGTACAATTCTTCACCTCGACGATTGTGTGAGAGCGCATTGAGGGAACCACCAAAACGGCCTGGTCAACAGCTTGTCATCCTTTCTCAGGTCCTAATGGACGATATCAATGCAGGATATGAACGTCTTGCAGAGTTACAGGTTAAGAAGGTTAACGGAGTTGAAGTTGACAATTTGAAACATTTATGCCTACTTGTCGAAAACTGCAGCGAAGAGAGCGTGCGCTTTGATTTGGATGATGATAGGGTTATTGTGTTGAATCGTCGCTTAGCAAAAGTTGCAACTTCAAAGATTTTGAAGTGCCACCGAATACCCTCAGTAATGTCTGATGATCTTACCATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

573

Amino Acids

62.74

Weight (kDa)

8.45

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 124 - 302 4.8e-18 Trypsin
Trypsin_2 PF13365 141 - 270 5.2e-21 Trypsin-like peptidase domain
PDZ_2 PF13180 333 - 417 7.3e-06 PDZ domain
PDZ_3 PF17815 424 - 568 9e-56 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 1150
AciI CCGC 9 cut(s) 75, 81, 126, 149, 153, 156, 159, 798, 1150
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 2 cut(s) 450, 1235
AcvI CACGTG 1 cut(s) 238
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 7 cut(s) 103, 115, 492, 524, 735, 1026, 1376
AfiI CCNNNNNNNGG 7 cut(s) 87, 117, 266, 674, 754, 1051, 1459
AhlI ACTAGT 2 cut(s) 92, 1027
AjnI CCWGG 4 cut(s) 29, 116, 784, 1432
AluBI AGCT 7 cut(s) 62, 131, 227, 328, 751, 1294, 1444
AluI AGCT 7 cut(s) 62, 131, 227, 328, 751, 1294, 1444
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 2 cut(s) 150, 1430
ApeKI GCWGC 7 cut(s) 128, 161, 227, 230, 369, 773, 1578
ApoI RAATTY 2 cut(s) 450, 1235
Asp700I GAANNNNTTC 2 cut(s) 353, 978
AspLEI GCGC 4 cut(s) 235, 1008, 1409, 1596
AspS9I GGNCC 3 cut(s) 300, 800, 1462
AsuC2I CCSGG 2 cut(s) 427, 1021
AsuHPI GGTGA 6 cut(s) 19, 287, 358, 689, 1235, 1377
AsuII TTCGAA 1 cut(s) 1138
AsuNHI GCTAGC 1 cut(s) 328
AvaII GGWCC 3 cut(s) 300, 800, 1462
BanII GRGCYC 2 cut(s) 425, 532
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BauI CACGAG 1 cut(s) 1355
BbrPI CACGTG 1 cut(s) 238
BbsI GAAGAC 2 cut(s) 159, 1374
BbvI GCAGC 7 cut(s) 140, 148, 214, 242, 381, 760, 1590
BccI CCATC 4 cut(s) 272, 1094, 1115, 1718
BceAI ACGGC 2 cut(s) 632, 1445
BcgI CGANNNNNNTGC 2 cut(s) 1463, 1497
BciT130I CCWGG 4 cut(s) 31, 118, 786, 1434
BciVI GTATCC 1 cut(s) 304
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 2 cut(s) 427, 1021
BcuI ACTAGT 2 cut(s) 92, 1027
BfaI CTAG 8 cut(s) 63, 93, 272, 329, 620, 711, 1028, 1299
BfmI CTRYAG 3 cut(s) 228, 1110, 1576
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 1 cut(s) 351
BlpI GCTNAGC 1 cut(s) 1639
Bme1390I CCNGG 6 cut(s) 31, 118, 427, 786, 1021, 1434
Bme18I GGWCC 3 cut(s) 300, 800, 1462
BmgT120I GGNCC 3 cut(s) 300, 800, 1462
BmiI GGNNCC 3 cut(s) 424, 992, 1419
BmrFI CCNGG 6 cut(s) 31, 118, 427, 786, 1021, 1434
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 4 cut(s) 307, 760, 993, 1045
BmtI GCTAGC 1 cut(s) 332
BmuI ACTGGG 1 cut(s) 881
BpiI GAAGAC 2 cut(s) 159, 1374
BpmI CTGGAG 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 1639
Bpu14I TTCGAA 1 cut(s) 1138
BpuEI CTTGAG 1 cut(s) 654
BpuMI CCSGG 2 cut(s) 427, 1021
BsaAI YACGTR 1 cut(s) 238
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 6 cut(s) 389, 417, 439, 648, 785, 1020
BsaXI ACNNNNNCTCC 4 cut(s) 1095, 1125, 1521, 1551
Bsc4I CCNNNNNNNGG 7 cut(s) 87, 117, 266, 674, 754, 1051, 1459
Bse1I ACTGG 5 cut(s) 111, 188, 887, 1274, 1376
BseBI CCWGG 4 cut(s) 31, 118, 786, 1434
BseDI CCNNGG 6 cut(s) 389, 417, 439, 648, 785, 1020
BseGI GGATG 5 cut(s) 288, 1008, 1060, 1449, 1612
BseLI CCNNNNNNNGG 7 cut(s) 87, 117, 266, 674, 754, 1051, 1459
BseMII CTCAG 5 cut(s) 977, 1205, 1355, 1472, 1701
BseNI ACTGG 5 cut(s) 111, 188, 887, 1274, 1376
BseRI GAGGAG 1 cut(s) 310
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 7 cut(s) 140, 148, 214, 242, 381, 760, 1590
BseYI CCCAGC 3 cut(s) 4, 405, 747
BsgI GTGCAG 1 cut(s) 876
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 152, 1432
BsiEI CGRYCG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 426, 1020
BslFI GGGAC 5 cut(s) 103, 388, 638, 869, 1232
BslI CCNNNNNNNGG 7 cut(s) 87, 117, 266, 674, 754, 1051, 1459
BsmFI GGGAC 5 cut(s) 103, 388, 638, 869, 1232
BsnI GGCC 2 cut(s) 152, 1432
Bsp119I TTCGAA 1 cut(s) 1138
Bsp1286I GDGCHC 2 cut(s) 425, 532
Bsp1407I TGTACA 1 cut(s) 733
Bsp143I GATC 4 cut(s) 351, 478, 616, 1702
Bsp1720I GCTNAGC 1 cut(s) 1639
Bsp19I CCATGG 1 cut(s) 417
BspACI CCGC 9 cut(s) 75, 81, 126, 149, 153, 156, 159, 798, 1150
BspANI GGCC 2 cut(s) 152, 1432
BspCNI CTCAG 5 cut(s) 978, 1206, 1354, 1471, 1700
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 3 cut(s) 424, 992, 1419
BspMAI CTGCAG 2 cut(s) 232, 1580
BspOI GCTAGC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 1138
BsrBI CCGCTC 1 cut(s) 1150
BsrGI TGTACA 1 cut(s) 733
BsrI ACTGG 5 cut(s) 111, 188, 887, 1274, 1376
BssECI CCNNGG 6 cut(s) 389, 417, 439, 648, 785, 1020
BssMI GATC 4 cut(s) 351, 478, 616, 1702
BssNI GRCGYC 1 cut(s) 250
BssSI CACGAG 1 cut(s) 1355
BssT1I CCWWGG 2 cut(s) 389, 417
Bst2BI CACGAG 1 cut(s) 1355
Bst2UI CCWGG 4 cut(s) 31, 118, 786, 1434
Bst4CI ACNGT 5 cut(s) 136, 361, 577, 1129, 1223
Bst6I CTCTTC 2 cut(s) 1221, 1578
BstACI GRCGYC 1 cut(s) 250
BstAPI GCANNNNNTGC 1 cut(s) 1649
BstAUI TGTACA 1 cut(s) 733
BstBAI YACGTR 1 cut(s) 238
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 2 cut(s) 330, 1592
BstDEI CTNAG 7 cut(s) 986, 1214, 1341, 1458, 1639, 1687, 1716
BstDSI CCRYGG 3 cut(s) 417, 439, 648
BstENI CCTNNNNNAGG 1 cut(s) 1457
BstF5I GGATG 5 cut(s) 288, 1008, 1060, 1449, 1612
BstHHI GCGC 4 cut(s) 235, 1008, 1409, 1596
BstKTI GATC 4 cut(s) 354, 481, 619, 1705
BstMBI GATC 4 cut(s) 351, 478, 616, 1702
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 7 cut(s) 68, 155, 158, 161, 833, 1587, 1649
BstNI CCWGG 4 cut(s) 31, 118, 786, 1434
BstSCI CCNGG 6 cut(s) 29, 116, 425, 784, 1019, 1432
BstSFI CTRYAG 3 cut(s) 228, 1110, 1576
BstV1I GCAGC 7 cut(s) 140, 148, 214, 242, 381, 760, 1590
BstV2I GAAGAC 2 cut(s) 159, 1374
BstX2I RGATCY 1 cut(s) 351
BstYI RGATCY 1 cut(s) 351
BstZI CGGCCG 1 cut(s) 150
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 2 cut(s) 152, 1432
BtgI CCRYGG 3 cut(s) 417, 439, 648
BtsCI GGATG 5 cut(s) 288, 1008, 1060, 1449, 1612
BtsIMutI CAGTG 2 cut(s) 348, 357
Cac8I GCNNGC 2 cut(s) 330, 1592
CaiI CAGNNNCTG 1 cut(s) 134
CciI TCATGA 1 cut(s) 550
CciNI GCGGCCGC 1 cut(s) 150
CfoI GCGC 4 cut(s) 235, 1008, 1409, 1596
Cfr13I GGNCC 3 cut(s) 300, 800, 1462
CseI GACGC 2 cut(s) 153, 258
Csp6I GTAC 7 cut(s) 102, 114, 491, 523, 734, 1025, 1375
CviAII CATG 7 cut(s) 418, 467, 506, 551, 754, 811, 960
CviQI GTAC 7 cut(s) 102, 114, 491, 523, 734, 1025, 1375
DdeI CTNAG 7 cut(s) 986, 1214, 1341, 1458, 1639, 1687, 1716
DpnI GATC 4 cut(s) 353, 480, 618, 1704
DpnII GATC 4 cut(s) 351, 478, 616, 1702
EaeI YGGCCR 1 cut(s) 150
EagI CGGCCG 1 cut(s) 150
Eam1104I CTCTTC 2 cut(s) 1221, 1578
EarI CTCTTC 2 cut(s) 1221, 1578
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 389, 417
Eco24I GRGCYC 2 cut(s) 425, 532
Eco32I GATATC 2 cut(s) 665, 1477
Eco47I GGWCC 3 cut(s) 300, 800, 1462
Eco52I CGGCCG 1 cut(s) 150
Eco72I CACGTG 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 1457
EcoO109I RGGNCCY 1 cut(s) 1462
EcoRI GAATTC 1 cut(s) 1235
EcoRII CCWGG 4 cut(s) 29, 116, 784, 1432
EcoRV GATATC 2 cut(s) 665, 1477
EcoT14I CCWWGG 2 cut(s) 389, 417
EcoT38I GRGCYC 2 cut(s) 425, 532
ErhI CCWWGG 2 cut(s) 389, 417
FaeI CATG 7 cut(s) 421, 470, 509, 554, 757, 814, 963
FaqI GGGAC 5 cut(s) 103, 388, 638, 869, 1232
FatI CATG 7 cut(s) 417, 466, 505, 550, 753, 810, 959
FbaI TGATCA 1 cut(s) 478
FokI GGATG 5 cut(s) 275, 1015, 1067, 1436, 1619
FriOI GRGCYC 2 cut(s) 425, 532
FspBI CTAG 8 cut(s) 63, 93, 272, 329, 620, 711, 1028, 1299
GlaI GCGC 4 cut(s) 234, 1007, 1408, 1595
GsaI CCCAGC 3 cut(s) 8, 409, 751
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 2 cut(s) 152, 1432
HapII CCGG 2 cut(s) 426, 1020
HgaI GACGC 2 cut(s) 153, 258
HhaI GCGC 4 cut(s) 235, 1008, 1409, 1596
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 7 cut(s) 421, 470, 509, 554, 757, 814, 963
Hin6I GCGC 4 cut(s) 233, 1006, 1407, 1594
HinP1I GCGC 4 cut(s) 233, 1006, 1407, 1594
HincII GTYRAC 5 cut(s) 193, 538, 1439, 1525, 1540
HindII GTYRAC 5 cut(s) 193, 538, 1439, 1525, 1540
HindIII AAGCTT 1 cut(s) 1292
HinfI GANTC 4 cut(s) 24, 338, 939, 1630
HpaI GTTAAC 3 cut(s) 193, 538, 1525
HpaII CCGG 2 cut(s) 426, 1020
HphI GGTGA 6 cut(s) 19, 287, 358, 689, 1235, 1377
Hpy166II GTNNAC 7 cut(s) 193, 538, 736, 1033, 1439, 1525, 1540
Hpy188I TCNGA 6 cut(s) 184, 456, 1002, 1054, 1215, 1699
Hpy188III TCNNGA 6 cut(s) 292, 551, 587, 841, 1069, 1355
Hpy8I GTNNAC 7 cut(s) 193, 538, 736, 1033, 1439, 1525, 1540
Hpy99I CGWCG 5 cut(s) 249, 252, 310, 1395, 1638
HpyAV CCTTC 4 cut(s) 395, 1259, 1361, 1513
HpyCH4III ACNGT 5 cut(s) 136, 361, 577, 1129, 1223
HpyCH4IV ACGT 4 cut(s) 11, 237, 262, 1495
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 155, 158, 161, 833, 1587, 1649
HpyF3I CTNAG 7 cut(s) 986, 1214, 1341, 1458, 1639, 1687, 1716
HpySE526I ACGT 4 cut(s) 11, 237, 262, 1495
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 7 cut(s) 421, 470, 509, 554, 757, 814, 963
HspAI GCGC 4 cut(s) 233, 1006, 1407, 1594
Ksp22I TGATCA 1 cut(s) 478
KspAI GTTAAC 3 cut(s) 193, 538, 1525
Kzo9I GATC 4 cut(s) 351, 478, 616, 1702
LmnI GCTCC 1 cut(s) 428
Lsp1109I GCAGC 7 cut(s) 140, 148, 214, 242, 381, 760, 1590
LweI GCATC 4 cut(s) 307, 760, 993, 1045
MaeI CTAG 8 cut(s) 63, 93, 272, 329, 620, 711, 1028, 1299
MaeII ACGT 4 cut(s) 11, 237, 262, 1495
MaeIII GTNAC 3 cut(s) 361, 691, 1506
MalI GATC 4 cut(s) 353, 480, 618, 1704
MbiI CCGCTC 1 cut(s) 1150
MboI GATC 4 cut(s) 351, 478, 616, 1702
MflI RGATCY 1 cut(s) 351
MhlI GDGCHC 2 cut(s) 425, 532
MluCI AATT 7 cut(s) 450, 765, 876, 1038, 1235, 1378, 1543
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 2 cut(s) 640, 916
MroXI GAANNNNTTC 2 cut(s) 353, 978
MseI TTAA 6 cut(s) 56, 192, 537, 1041, 1515, 1524
MslI CAYNNNNRTG 2 cut(s) 555, 1359
MspA1I CMGCKG 2 cut(s) 161, 227
MspI CCGG 2 cut(s) 426, 1020
MspR9I CCNGG 6 cut(s) 31, 118, 427, 786, 1021, 1434
MvaI CCWGG 4 cut(s) 31, 118, 786, 1434
MwoI GCNNNNNNNGC 7 cut(s) 68, 155, 158, 161, 833, 1587, 1649
NciI CCSGG 2 cut(s) 427, 1021
NcoI CCATGG 1 cut(s) 417
NdeII GATC 4 cut(s) 351, 478, 616, 1702
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 7 cut(s) 421, 470, 509, 554, 757, 814, 963
NlaIV GGNNCC 3 cut(s) 424, 992, 1419
NmuCI GTSAC 1 cut(s) 361
NotI GCGGCCGC 1 cut(s) 150
NspV TTCGAA 1 cut(s) 1138
PagI TCATGA 1 cut(s) 550
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PdmI GAANNNNTTC 2 cut(s) 353, 978
PfeI GAWTC 3 cut(s) 338, 939, 1630
PflMI CCANNNNNTGG 1 cut(s) 754
PleI GAGTC 1 cut(s) 18
PmaCI CACGTG 1 cut(s) 238
PmlI CACGTG 1 cut(s) 238
PpsI GAGTC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 1462
Psp5II RGGWCCY 1 cut(s) 1462
Psp6I CCWGG 4 cut(s) 29, 116, 784, 1432
PspCI CACGTG 1 cut(s) 238
PspFI CCCAGC 3 cut(s) 4, 405, 747
PspGI CCWGG 4 cut(s) 29, 116, 784, 1432
PspN4I GGNNCC 3 cut(s) 424, 992, 1419
PspPI GGNCC 3 cut(s) 300, 800, 1462
PspPPI RGGWCCY 1 cut(s) 1462
PstI CTGCAG 2 cut(s) 232, 1580
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 7 cut(s) 103, 115, 492, 524, 735, 1026, 1376
RsaNI GTAC 7 cut(s) 102, 114, 491, 523, 734, 1025, 1375
RseI CAYNNNNRTG 2 cut(s) 555, 1359
SaqAI TTAA 6 cut(s) 56, 192, 537, 1041, 1515, 1524
Sau3AI GATC 4 cut(s) 351, 478, 616, 1702
Sau96I GGNCC 3 cut(s) 300, 800, 1462
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 6 cut(s) 31, 118, 427, 786, 1021, 1434
SduI GDGCHC 2 cut(s) 425, 532
SfaNI GCATC 4 cut(s) 307, 760, 993, 1045
SfcI CTRYAG 3 cut(s) 228, 1110, 1576
SfuI TTCGAA 1 cut(s) 1138
SinI GGWCC 3 cut(s) 300, 800, 1462
SmiMI CAYNNNNRTG 2 cut(s) 555, 1359
SmlI CTYRAG 1 cut(s) 669
SmoI CTYRAG 1 cut(s) 669
SpeI ACTAGT 2 cut(s) 92, 1027
Sse9I AATT 7 cut(s) 450, 765, 876, 1038, 1235, 1378, 1543
SsiI CCGC 9 cut(s) 75, 81, 126, 149, 153, 156, 159, 798, 1150
SspI AATATT 1 cut(s) 865
SspMI CTAG 8 cut(s) 63, 93, 272, 329, 620, 711, 1028, 1299
StyD4I CCNGG 6 cut(s) 29, 116, 425, 784, 1019, 1432
StyI CCWWGG 2 cut(s) 389, 417
TaaI ACNGT 5 cut(s) 136, 361, 577, 1129, 1223
TaiI ACGT 4 cut(s) 14, 240, 265, 1498
TaqI TCGA 5 cut(s) 615, 1138, 1287, 1390, 1569
TasI AATT 7 cut(s) 450, 765, 876, 1038, 1235, 1378, 1543
TatI WGTACW 4 cut(s) 101, 490, 522, 733
TauI GCSGC 5 cut(s) 128, 152, 155, 158, 161
TfiI GAWTC 3 cut(s) 338, 939, 1630
Tru1I TTAA 6 cut(s) 56, 192, 537, 1041, 1515, 1524
Tru9I TTAA 6 cut(s) 56, 192, 537, 1041, 1515, 1524
TscAI CASTG 2 cut(s) 348, 364
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 7 cut(s) 128, 161, 227, 230, 369, 773, 1578
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 6 cut(s) 567, 1121, 1193, 1248, 1268, 1506
TspGWI ACGGA 4 cut(s) 4, 323, 428, 1542
TspRI CASTG 2 cut(s) 348, 364
Van91I CCANNNNNTGG 1 cut(s) 754
VpaK11BI GGWCC 3 cut(s) 300, 800, 1462
XagI CCTNNNNNAGG 1 cut(s) 1457
XapI RAATTY 2 cut(s) 450, 1235
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 353, 978
XspI CTAG 8 cut(s) 63, 93, 272, 329, 620, 711, 1028, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.