Rh5AG000100

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014559
Physical Location & Seq
Reverse (-)
28264 .. 35194
6931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG000100.1

Sequence Viewer

Length: 1668 bp
ATGCTGGTTACGTCCGCCCGAACACTCCTCAGAAGGCGGCTATGCTCTATTGAGTTGTTAAAAGATTATACGTCAAGGAGAAGTGGTATTAGCTGTAGTAGCAGTACCTGGGCGGCAGCTAATGTTGAAGAAGATGCTGCTGCTGCTTCTTGTTCCTCGTCTGAGTCGGTGTCGGTCGCCTCCCCCTCAGTCAGGAATAATATGCTCCGACGACGACGTTTCGGTATTCGGAGGAACAACGAGAACCGGAGCACAAAGGATACGGATGCCGTAGAGCTGGCATTGGACTCAGTGGTGAAGATCTTTACTGTGGCGAGCAGCCCTAACTACTTCCTGCCCTGGCAGAACAAGTCCCAACGAGAGACCATGGGTTCCGGATTTGTAATCGCCGGCAAGAAAATTCTTACAAACGCTCATGTAGTTGCTGATCATACATTTGTTCTGGTAAGAAAGCATGGCTCGCCTACCAAGTATAAAGCGCAAGTTGAAGCTGTTGGTCATGAATGTGATTTAGCCATTTTGAGTGTTGAAACTGAAAAGTTCTGGGAGGGTATGAATTCCCTTGAGCTAGGGGATATTCCTTTTCTGCAAGAAGCTGTTGCTGTTGTTGGATATCCTCAAGGTGGGGACAATATCTCTGTTACTAAAGGTGTTGTTTCCAGGGTTGAACCTACACAATATGTTCATGGTGCAACCCAGCTTATGGCAATACAGATTGATGCAGCAATAAATCCCGGAAATAGTGGTGGCCCAGCAATCATGGGAAATAAAGTTGCTGGTGTTGCTTTCCAAAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTTCCTGTAATAAAGCATTTTATTGCTGGAGTTGAAGAAAGAGGTAAATATGTTGGATTCTGCTCTCTAGGTTTGTCATGCCAGCCTACTGAAAATGTTCAACTCCGGAACCACTTTCAAATGCGTCCTGAAATGACTGGGGTACTAGTGAGCAAAATCAACCCTCTTTCGGATGCCCACAAAGTCTTAAAGAAAGATGATGTTGTTCTTTTCTTTGATGGAGTTCCTGTAGCAAATGATGGAACAGTTCACTTCAGGAACAGAGAGCGGATAACATTTGATCATTTGGTGTCCATGAAGAAACCCAATGAAACAGCTGTAGTTAGAGTTTTGAGGGATGGCGAAGAATATGAATTCACTATCACCCTTCAACCTTTACAACCGTTAGTTCCAGTTCATCAGTTCGATAAACTTCCTAGTTATTACATTTTTGCTGGTTTGGTCTTTGTTCCACTCACTCAACCTTACCTTCATGAGTATGGAGAAGATTGGTATAATACTTCACCTCGTAAATTATGCGAAATCGCATTGAGGGAACCACCTAAAAGAGCTGGTGAACAGATTGTCATCCTTTCTCAGGTACTCATGGATGATATCAATGCTGGATATGAACGTCTTGCAGAGCTGCAGGTTAAGAAGGTTAACGGGATAGAAGTTGAAAATTTGAAACATTTATGCCAACTTGTCGAGAACTGTAGTGAAGAGAGCGTGCGTTTTGATTTGGATGATGATAGAGTTGTTGTGTTGAACTATCATTTAGCAAAAGTTGCGACTTCAAAAATTCTAAATTGCCACAGGATACCCTCTGCAATGTCTAATGATCTTTTAGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

555

Amino Acids

61.57

Weight (kDa)

6.59

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 107 - 285 3.9e-18 Trypsin
Trypsin_2 PF13365 124 - 253 6e-21 Trypsin-like peptidase domain
PDZ_2 PF13180 317 - 400 4.1e-06 PDZ domain
PDZ_3 PF17815 407 - 551 6.5e-56 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1450
AccB7I CCANNNNNTGG 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 1099
AccIII TCCGGA 2 cut(s) 374, 936
AciI CCGC 4 cut(s) 15, 37, 113, 1099
AcsI RAATTY 5 cut(s) 399, 556, 1184, 1492, 1611
AcuI CTGAAG 1 cut(s) 1069
AfaI GTAC 3 cut(s) 106, 975, 1413
AfiI CCNNNNNNNGG 5 cut(s) 192, 623, 703, 1000, 1408
AhlI ACTAGT 1 cut(s) 976
AjnI CCWGG 3 cut(s) 107, 338, 659
Alw21I GWGCWC 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 356
AlwNI CAGNNNCTG 1 cut(s) 108
Aor13HI TCCGGA 2 cut(s) 374, 936
AoxI GGCC 1 cut(s) 748
ApeKI GCWGC 7 cut(s) 116, 137, 140, 143, 318, 722, 1456
ApoI RAATTY 5 cut(s) 399, 556, 1184, 1492, 1611
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 1 cut(s) 735
AsuHPI GGTGA 4 cut(s) 307, 1186, 1326, 1397
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 7 cut(s) 124, 127, 128, 130, 330, 734, 1443
BccI CCATC 3 cut(s) 1043, 1064, 1163
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 3 cut(s) 109, 340, 661
BciVI GTATCC 2 cut(s) 253, 1623
BclI TGATCA 2 cut(s) 427, 1111
BcnI CCSGG 1 cut(s) 735
BcoDI GTCTC 1 cut(s) 356
BcuI ACTAGT 1 cut(s) 976
BfaI CTAG 4 cut(s) 569, 899, 977, 1248
BfmI CTRYAG 5 cut(s) 94, 1059, 1149, 1457, 1525
BfuAI ACCTGC 1 cut(s) 1450
BfuI GTATCC 2 cut(s) 253, 1623
BglII AGATCT 1 cut(s) 300
BisI GCNGC 9 cut(s) 38, 114, 117, 138, 141, 144, 319, 723, 1457
BlsI GCNGC 9 cut(s) 39, 115, 118, 139, 142, 145, 320, 724, 1458
Bme1390I CCNGG 4 cut(s) 109, 340, 661, 735
BmgT120I GGNCC 1 cut(s) 749
BmiI GGNNCC 3 cut(s) 373, 941, 1368
BmrFI CCNGG 4 cut(s) 109, 340, 661, 735
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 4 cut(s) 124, 256, 709, 994
BmuI ACTGGG 1 cut(s) 978
BpmI CTGGAG 1 cut(s) 879
BpuEI CTTGAG 2 cut(s) 584, 603
BpuMI CCSGG 1 cut(s) 735
BsaBI GATNNNNATC 1 cut(s) 1397
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 4 cut(s) 108, 338, 366, 660
BsaWI WCCGGW 3 cut(s) 246, 374, 936
BsaXI ACNNNNNCTCC 4 cut(s) 70, 100, 1044, 1074
Bsc4I CCNNNNNNNGG 5 cut(s) 192, 623, 703, 1000, 1408
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 973, 1223
Bse3DI GCAATG 1 cut(s) 1647
Bse8I GATNNNNATC 1 cut(s) 1397
BseAI TCCGGA 2 cut(s) 374, 936
BseBI CCWGG 3 cut(s) 109, 340, 661
BseDI CCNNGG 4 cut(s) 108, 338, 366, 660
BseGI GGATG 6 cut(s) 271, 1009, 1174, 1398, 1426, 1561
BseJI GATNNNNATC 1 cut(s) 1397
BseLI CCNNNNNNNGG 5 cut(s) 192, 623, 703, 1000, 1408
BseMI GCAATG 1 cut(s) 1647
BseMII CTCAG 5 cut(s) 43, 153, 201, 303, 1421
BseNI ACTGG 2 cut(s) 973, 1223
BseRI GAGGAG 1 cut(s) 17
BseXI GCAGC 7 cut(s) 124, 127, 128, 130, 330, 734, 1443
BseYI CCCAGC 2 cut(s) 696, 751
BsgI GTGCAG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 177
BshFI GGCC 1 cut(s) 750
BsiEI CGRYCG 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 254
BsiSI CCGG 5 cut(s) 247, 375, 390, 735, 937
BslFI GGGAC 2 cut(s) 337, 641
BslI CCNNNNNNNGG 5 cut(s) 192, 623, 703, 1000, 1408
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 2 cut(s) 337, 641
BsnI GGCC 1 cut(s) 750
Bso31I GGTCTC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 254
Bsp13I TCCGGA 2 cut(s) 374, 936
Bsp143I GATC 4 cut(s) 300, 427, 1111, 1651
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 4 cut(s) 15, 37, 113, 1099
BspANI GGCC 1 cut(s) 750
BspCNI CTCAG 5 cut(s) 42, 154, 200, 302, 1420
BspEI TCCGGA 2 cut(s) 374, 936
BspHI TCATGA 2 cut(s) 499, 1303
BspLI GGNNCC 3 cut(s) 373, 941, 1368
BspMAI CTGCAG 1 cut(s) 1461
BspMI ACCTGC 1 cut(s) 1450
BspTNI GGTCTC 1 cut(s) 356
BsrBI CCGCTC 1 cut(s) 1099
BsrDI GCAATG 1 cut(s) 1647
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 2 cut(s) 973, 1223
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 4 cut(s) 108, 338, 366, 660
BssMI GATC 4 cut(s) 300, 427, 1111, 1651
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 109, 340, 661
Bst4CI ACNGT 4 cut(s) 310, 1078, 1215, 1526
Bst6I CTCTTC 1 cut(s) 1527
BstAPI GCANNNNNTGC 1 cut(s) 1598
BstC8I GCNNGC 6 cut(s) 279, 316, 391, 461, 914, 1541
BstDEI CTNAG 5 cut(s) 29, 162, 187, 289, 1407
BstDSI CCRYGG 1 cut(s) 366
BstENI CCTNNNNNAGG 2 cut(s) 190, 1406
BstF5I GGATG 6 cut(s) 271, 1009, 1174, 1398, 1426, 1561
BstHHI GCGC 1 cut(s) 481
BstKTI GATC 4 cut(s) 303, 430, 1114, 1654
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 4 cut(s) 300, 427, 1111, 1651
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 5 cut(s) 99, 143, 460, 782, 1598
BstNI CCWGG 3 cut(s) 109, 340, 661
BstSCI CCNGG 4 cut(s) 107, 338, 659, 733
BstSFI CTRYAG 5 cut(s) 94, 1059, 1149, 1457, 1525
BstV1I GCAGC 7 cut(s) 124, 127, 128, 130, 330, 734, 1443
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BsuI GTATCC 2 cut(s) 253, 1623
BsuRI GGCC 1 cut(s) 750
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 6 cut(s) 271, 1009, 1174, 1398, 1426, 1561
BtsIMutI CAGTG 1 cut(s) 297
BveI ACCTGC 1 cut(s) 1450
Cac8I GCNNGC 6 cut(s) 279, 316, 391, 461, 914, 1541
CaiI CAGNNNCTG 1 cut(s) 108
CciI TCATGA 2 cut(s) 499, 1303
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 749
CseI GACGC 1 cut(s) 944
Csp6I GTAC 3 cut(s) 105, 974, 1412
CviQI GTAC 3 cut(s) 105, 974, 1412
DdeI CTNAG 5 cut(s) 29, 162, 187, 289, 1407
DpnI GATC 4 cut(s) 302, 429, 1113, 1653
DpnII GATC 4 cut(s) 300, 427, 1111, 1651
Eam1104I CTCTTC 1 cut(s) 1527
EarI CTCTTC 1 cut(s) 1527
EciI GGCGGA 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 2 cut(s) 614, 1426
Eco57I CTGAAG 1 cut(s) 1069
EcoNI CCTNNNNNAGG 2 cut(s) 190, 1406
EcoRI GAATTC 2 cut(s) 556, 1184
EcoRII CCWGG 3 cut(s) 107, 338, 659
EcoRV GATATC 2 cut(s) 614, 1426
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaqI GGGAC 2 cut(s) 337, 641
FbaI TGATCA 2 cut(s) 427, 1111
Fnu4HI GCNGC 9 cut(s) 38, 114, 117, 138, 141, 144, 319, 723, 1457
FokI GGATG 6 cut(s) 278, 1016, 1181, 1385, 1433, 1568
Fsp4HI GCNGC 9 cut(s) 38, 114, 117, 138, 141, 144, 319, 723, 1457
FspBI CTAG 4 cut(s) 569, 899, 977, 1248
GlaI GCGC 1 cut(s) 480
GluI GCNGC 9 cut(s) 38, 114, 117, 138, 141, 144, 319, 723, 1457
GsaI CCCAGC 2 cut(s) 700, 755
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 1 cut(s) 750
HapII CCGG 5 cut(s) 247, 375, 390, 735, 937
HgaI GACGC 1 cut(s) 944
HhaI GCGC 1 cut(s) 481
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HincII GTYRAC 1 cut(s) 1474
HindII GTYRAC 1 cut(s) 1474
HinfI GANTC 3 cut(s) 164, 287, 888
HpaI GTTAAC 1 cut(s) 1474
HpaII CCGG 5 cut(s) 247, 375, 390, 735, 937
HphI GGTGA 4 cut(s) 307, 1186, 1326, 1397
Hpy166II GTNNAC 3 cut(s) 1081, 1388, 1474
Hpy188I TCNGA 5 cut(s) 32, 163, 209, 231, 1003
Hpy188III TCNNGA 8 cut(s) 193, 375, 500, 937, 959, 1087, 1304, 1519
Hpy8I GTNNAC 3 cut(s) 1081, 1388, 1474
Hpy99I CGWCG 3 cut(s) 213, 216, 219
HpyAV CCTTC 4 cut(s) 27, 1208, 1310, 1462
HpyCH4III ACNGT 4 cut(s) 310, 1078, 1215, 1526
HpyCH4IV ACGT 4 cut(s) 11, 71, 217, 1444
HpyCH4V TGCA 7 cut(s) 589, 692, 722, 806, 1451, 1459, 1640
HpyF10VI GCNNNNNNNGC 5 cut(s) 99, 143, 460, 782, 1598
HpyF3I CTNAG 5 cut(s) 29, 162, 187, 289, 1407
HpySE526I ACGT 4 cut(s) 11, 71, 217, 1444
HspAI GCGC 1 cut(s) 479
Kpn2I TCCGGA 2 cut(s) 374, 936
KroI GCCGGC 1 cut(s) 389
KroNI GCCGGC 1 cut(s) 391
Ksp22I TGATCA 2 cut(s) 427, 1111
KspAI GTTAAC 1 cut(s) 1474
Kzo9I GATC 4 cut(s) 300, 427, 1111, 1651
LmnI GCTCC 2 cut(s) 210, 249
Lsp1109I GCAGC 7 cut(s) 124, 127, 128, 130, 330, 734, 1443
LweI GCATC 4 cut(s) 124, 256, 709, 994
MaeI CTAG 4 cut(s) 569, 899, 977, 1248
MaeII ACGT 4 cut(s) 11, 71, 217, 1444
MaeIII GTNAC 2 cut(s) 7, 640
MalI GATC 4 cut(s) 302, 429, 1113, 1653
MbiI CCGCTC 1 cut(s) 1099
MboI GATC 4 cut(s) 300, 427, 1111, 1651
MboII GAAGA 8 cut(s) 140, 143, 310, 878, 1141, 1187, 1328, 1544
MflI RGATCY 1 cut(s) 300
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 8 cut(s) 399, 556, 825, 1184, 1343, 1492, 1611, 1618
MlyI GAGTC 2 cut(s) 173, 281
MmeI TCCRAC 3 cut(s) 232, 589, 865
MroI TCCGGA 2 cut(s) 374, 936
MroNI GCCGGC 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 4 cut(s) 59, 1019, 1464, 1473
MslI CAYNNNNRTG 2 cut(s) 504, 1308
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 5 cut(s) 247, 375, 390, 735, 937
MspR9I CCNGG 4 cut(s) 109, 340, 661, 735
MvaI CCWGG 3 cut(s) 109, 340, 661
MwoI GCNNNNNNNGC 5 cut(s) 99, 143, 460, 782, 1598
NaeI GCCGGC 1 cut(s) 391
NciI CCSGG 1 cut(s) 735
NcoI CCATGG 1 cut(s) 366
NdeII GATC 4 cut(s) 300, 427, 1111, 1651
NgoMIV GCCGGC 1 cut(s) 389
NlaIV GGNNCC 3 cut(s) 373, 941, 1368
PagI TCATGA 2 cut(s) 499, 1303
PdiI GCCGGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 1 cut(s) 888
PflMI CCANNNNNTGG 1 cut(s) 703
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 9 cut(s) 39, 115, 118, 139, 142, 145, 320, 724, 1458
PleI GAGTC 2 cut(s) 172, 281
PpsI GAGTC 2 cut(s) 172, 281
Psp6I CCWGG 3 cut(s) 107, 338, 659
PspFI CCCAGC 2 cut(s) 696, 751
PspGI CCWGG 3 cut(s) 107, 338, 659
PspN4I GGNNCC 3 cut(s) 373, 941, 1368
PspPI GGNCC 1 cut(s) 749
PstI CTGCAG 1 cut(s) 1461
PstNI CAGNNNCTG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 1148
RsaI GTAC 3 cut(s) 106, 975, 1413
RsaNI GTAC 3 cut(s) 105, 974, 1412
RseI CAYNNNNRTG 2 cut(s) 504, 1308
SaqAI TTAA 4 cut(s) 59, 1019, 1464, 1473
SatI GCNGC 9 cut(s) 38, 114, 117, 138, 141, 144, 319, 723, 1457
Sau3AI GATC 4 cut(s) 300, 427, 1111, 1651
Sau96I GGNCC 1 cut(s) 749
SchI GAGTC 2 cut(s) 173, 281
ScrFI CCNGG 4 cut(s) 109, 340, 661, 735
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 4 cut(s) 124, 256, 709, 994
SfcI CTRYAG 5 cut(s) 94, 1059, 1149, 1457, 1525
SmiMI CAYNNNNRTG 2 cut(s) 504, 1308
SmlI CTYRAG 2 cut(s) 563, 618
SmoI CTYRAG 2 cut(s) 563, 618
SpeI ACTAGT 1 cut(s) 976
Sse9I AATT 8 cut(s) 399, 556, 825, 1184, 1343, 1492, 1611, 1618
SsiI CCGC 4 cut(s) 15, 37, 113, 1099
SspI AATATT 1 cut(s) 814
SspMI CTAG 4 cut(s) 569, 899, 977, 1248
StyD4I CCNGG 4 cut(s) 107, 338, 659, 733
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 4 cut(s) 310, 1078, 1215, 1526
TaiI ACGT 4 cut(s) 14, 74, 220, 1447
TaqI TCGA 2 cut(s) 1236, 1518
TaqII GACCGA 1 cut(s) 163
TasI AATT 8 cut(s) 399, 556, 825, 1184, 1343, 1492, 1611, 1618
TauI GCSGC 2 cut(s) 40, 116
TfiI GAWTC 1 cut(s) 888
Tru1I TTAA 4 cut(s) 59, 1019, 1464, 1473
Tru9I TTAA 4 cut(s) 59, 1019, 1464, 1473
TscAI CASTG 1 cut(s) 297
TseI GCWGC 7 cut(s) 116, 137, 140, 143, 318, 722, 1456
TspDTI ATGAA 9 cut(s) 516, 569, 674, 1142, 1155, 1197, 1217, 1292, 1455
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 703
XagI CCTNNNNNAGG 2 cut(s) 190, 1406
XapI RAATTY 5 cut(s) 399, 556, 1184, 1492, 1611
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 4 cut(s) 569, 899, 977, 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.