Rmu_sc0007644.1_g000006

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007644.1
OG0014559
Physical Location & Seq
Reverse (-)
15890 .. 22781
6892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007644.1_g000006.1.cds

Sequence Viewer

Length: 1824 bp
atgaaacgtttggaggtgttgggtctctcttgcggcgctagggctaaccatagaatgttgggacgtgttcggatggaaatagcaggaggccttgaaaaccagaacactagggaactcaactccggctgcccagcaaagcgctgctgtgttaaaatgctggttacgtccgcccgaacactcctcagaaggcggctatgctctattgagttgctaaaagattctacgtcaaggagaagtggtattaactgtagtagcagtacctgggcggcagctaatgttgaagaagatgctgctgctgctgcttcttgttcctcgtctgagtcggtgtcggtcgcctccccctcagtcaggaataatatgctccgacgacgacgtttcggtattcggaggaacaacgagaaccggagcacaaaggatacggatgccgtagagctggcattggactcagtggtgaagatctttactgtggcgagcagccctaactacttcctgccctggcagaacaagtcccaacgagagaccatgggttccggatttgtaatggccggcaagaaaattcttacaaacgctcatgtagttgctgatcatacatttgttctagtaagaaagcatggctcgcctaccaagtataaagcgcaagttgaagctgttggtcatgaatgtgatttagccattttgagtgttgaaactgaaaagttctgggagggtatgaattcccttgagctaggggatattccttttctgcaagaagctgttgctgttgttggatatcctcaaggtggggacaatatctctgttactaaaggtgttgtttccagggttgaacctacacaatacgttcatggtgcaacccagcttatggcaatacagattgatgcagcaataaatcccggaaatagtggtggcccagcaatcatgggaaataaagttgctggtgttgctttccaaaacctttctggggcagagaatattggttatataattcctgttcctgtaataaagcattttattgctggagttgaagaaagaggtaaatatgttggattctgctctctaggtttgtcatgccagcctactgaaaatgttcaactccggaaccactttcaaatgcgtcctgaaatgactggggtactagtgagcaaaatcaaccctctttcggatgcccacaaagtcttaaagaaagatgatgttgttcttttctttgatggagttcctgtagcaaatgatggaacagttcacttcaggaacagagagcggataacatttgatcatttggtgtccatgaagaaacccaatgaaacagctgtagttagagttttgagggatggcgaagaatatgaattcagtatcacccttcaacctttacaaccgttagttccagttcatcagttcgataaacttcctagttattacatttttgctggtttggtctttgttccgctcactcaaccttaccttcatgagtatggagaagattggtataatacttcacctcgtaaattatgcgaaatcgcattgagggaaccacctaaaagagctggtgaacagattgtcatcctttctcaggtactcatggatgatatcaatgctggatatgaacgtcttgcagagctgcaggttaagaaggttaacgggatagaagttgaaaatttgaaacatttatgccaacttgtcgaaaactgtagtgaagagaacgtgcgttttgatttggatgatgatagagttgttgtgttgaactatcatttagcaaaagttgcgacttcaaaaattctaaattgccacaggataccctctgcaatgtctaatgatcttttagagaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

607

Amino Acids

67.23

Weight (kDa)

8.11

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1606
AccB7I CCANNNNNTGG 1 cut(s) 859
AccBSI CCGCTC 2 cut(s) 1255, 1441
AccIII TCCGGA 2 cut(s) 530, 1092
AciI CCGC 6 cut(s) 33, 168, 190, 266, 1255, 1439
AclI AACGTT 1 cut(s) 7
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 5 cut(s) 555, 712, 1340, 1648, 1767
AcuI CTGAAG 1 cut(s) 1225
AfaI GTAC 3 cut(s) 259, 1131, 1569
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 6 cut(s) 348, 779, 859, 958, 1156, 1564
AflIII ACRYGT 1 cut(s) 64
AhlI ACTAGT 1 cut(s) 1132
AjiI CACGTC 1 cut(s) 65
AjnI CCWGG 3 cut(s) 260, 494, 815
AluBI AGCT 9 cut(s) 272, 433, 647, 724, 752, 856, 1304, 1538, 1612
AluI AGCT 9 cut(s) 272, 433, 647, 724, 752, 856, 1304, 1538, 1612
Alw21I GWGCWC 1 cut(s) 410
Alw26I GTCTC 2 cut(s) 29, 512
AlwNI CAGNNNCTG 1 cut(s) 261
Aor13HI TCCGGA 2 cut(s) 530, 1092
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 3 cut(s) 88, 543, 904
ApoI RAATTY 5 cut(s) 555, 712, 1340, 1648, 1767
Asp700I GAANNNNTTC 1 cut(s) 1083
AspLEI GCGC 3 cut(s) 38, 141, 637
AspS9I GGNCC 1 cut(s) 905
AsuC2I CCSGG 1 cut(s) 891
AsuHPI GGTGA 4 cut(s) 463, 1342, 1482, 1553
Bbv12I GWGCWC 1 cut(s) 410
BccI CCATC 4 cut(s) 67, 1199, 1220, 1319
BceAI ACGGC 1 cut(s) 410
BciT130I CCWGG 3 cut(s) 262, 496, 817
BciVI GTATCC 2 cut(s) 409, 1779
BclI TGATCA 2 cut(s) 583, 1267
BcnI CCSGG 1 cut(s) 891
BcoDI GTCTC 2 cut(s) 29, 512
BcuI ACTAGT 1 cut(s) 1132
BfaI CTAG 7 cut(s) 39, 108, 599, 725, 1055, 1133, 1404
BfmI CTRYAG 5 cut(s) 247, 1215, 1305, 1613, 1681
BfoI RGCGCY 2 cut(s) 39, 142
BfuAI ACCTGC 1 cut(s) 1606
BfuI GTATCC 2 cut(s) 409, 1779
BglII AGATCT 1 cut(s) 456
Bme1390I CCNGG 4 cut(s) 262, 496, 817, 891
BmgBI CACGTC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 905
BmiI GGNNCC 3 cut(s) 529, 1097, 1524
BmrFI CCNGG 4 cut(s) 262, 496, 817, 891
BmrI ACTGGG 1 cut(s) 1134
BmsI GCATC 4 cut(s) 277, 412, 865, 1150
BmuI ACTGGG 1 cut(s) 1134
BpmI CTGGAG 1 cut(s) 1035
BpuEI CTTGAG 2 cut(s) 740, 759
BpuMI CCSGG 1 cut(s) 891
BsaBI GATNNNNATC 1 cut(s) 1553
BsaI GGTCTC 2 cut(s) 29, 512
BsaJI CCNNGG 4 cut(s) 261, 494, 522, 816
BsaWI WCCGGW 3 cut(s) 402, 530, 1092
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 1200, 1230
Bsc4I CCNNNNNNNGG 6 cut(s) 348, 779, 859, 958, 1156, 1564
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 2 cut(s) 1129, 1379
Bse3DI GCAATG 1 cut(s) 1803
Bse8I GATNNNNATC 1 cut(s) 1553
BseAI TCCGGA 2 cut(s) 530, 1092
BseBI CCWGG 3 cut(s) 262, 496, 817
BseDI CCNNGG 4 cut(s) 261, 494, 522, 816
BseGI GGATG 7 cut(s) 78, 427, 1165, 1330, 1554, 1582, 1717
BseJI GATNNNNATC 1 cut(s) 1553
BseLI CCNNNNNNNGG 6 cut(s) 348, 779, 859, 958, 1156, 1564
BseMI GCAATG 1 cut(s) 1803
BseMII CTCAG 5 cut(s) 196, 309, 357, 459, 1577
BseNI ACTGG 2 cut(s) 1129, 1379
BseRI GAGGAG 1 cut(s) 170
BseYI CCCAGC 3 cut(s) 130, 852, 907
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 3 cut(s) 90, 545, 906
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 410
BsiSI CCGG 6 cut(s) 123, 403, 531, 546, 891, 1093
BslFI GGGAC 3 cut(s) 75, 493, 797
BslI CCNNNNNNNGG 6 cut(s) 348, 779, 859, 958, 1156, 1564
BsmAI GTCTC 2 cut(s) 29, 512
BsmFI GGGAC 3 cut(s) 75, 493, 797
BsnI GGCC 3 cut(s) 90, 545, 906
Bso31I GGTCTC 2 cut(s) 29, 512
Bsp1286I GDGCHC 1 cut(s) 410
Bsp13I TCCGGA 2 cut(s) 530, 1092
Bsp143I GATC 4 cut(s) 456, 583, 1267, 1807
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 6 cut(s) 33, 168, 190, 266, 1255, 1439
BspANI GGCC 3 cut(s) 90, 545, 906
BspCNI CTCAG 5 cut(s) 195, 310, 356, 458, 1576
BspEI TCCGGA 2 cut(s) 530, 1092
BspHI TCATGA 2 cut(s) 655, 1459
BspLI GGNNCC 3 cut(s) 529, 1097, 1524
BspMAI CTGCAG 1 cut(s) 1617
BspMI ACCTGC 1 cut(s) 1606
BspTNI GGTCTC 2 cut(s) 29, 512
BsrBI CCGCTC 2 cut(s) 1255, 1441
BsrDI GCAATG 1 cut(s) 1803
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 2 cut(s) 1129, 1379
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 4 cut(s) 261, 494, 522, 816
BssMI GATC 4 cut(s) 456, 583, 1267, 1807
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 3 cut(s) 262, 496, 817
Bst4CI ACNGT 5 cut(s) 248, 466, 1234, 1371, 1682
Bst6I CTCTTC 1 cut(s) 1683
BstAPI GCANNNNNTGC 1 cut(s) 1754
BstC8I GCNNGC 5 cut(s) 435, 472, 547, 617, 1070
BstDEI CTNAG 5 cut(s) 182, 318, 343, 445, 1563
BstDSI CCRYGG 1 cut(s) 522
BstENI CCTNNNNNAGG 2 cut(s) 346, 1562
BstF5I GGATG 7 cut(s) 78, 427, 1165, 1330, 1554, 1582, 1717
BstH2I RGCGCY 2 cut(s) 39, 142
BstHHI GCGC 3 cut(s) 38, 141, 637
BstKTI GATC 4 cut(s) 459, 586, 1270, 1810
BstMAI GTCTC 2 cut(s) 29, 512
BstMBI GATC 4 cut(s) 456, 583, 1267, 1807
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 5 cut(s) 296, 299, 616, 938, 1754
BstNI CCWGG 3 cut(s) 262, 496, 817
BstSCI CCNGG 4 cut(s) 260, 494, 815, 889
BstSFI CTRYAG 5 cut(s) 247, 1215, 1305, 1613, 1681
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BsuI GTATCC 2 cut(s) 409, 1779
BsuRI GGCC 3 cut(s) 90, 545, 906
BtgI CCRYGG 1 cut(s) 522
BtrI CACGTC 1 cut(s) 65
BtsCI GGATG 7 cut(s) 78, 427, 1165, 1330, 1554, 1582, 1717
BtsIMutI CAGTG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 1606
Cac8I GCNNGC 5 cut(s) 435, 472, 547, 617, 1070
CaiI CAGNNNCTG 1 cut(s) 261
CciI TCATGA 2 cut(s) 655, 1459
CfoI GCGC 3 cut(s) 38, 141, 637
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 905
CseI GACGC 1 cut(s) 1100
Csp6I GTAC 3 cut(s) 258, 1130, 1568
CviQI GTAC 3 cut(s) 258, 1130, 1568
DdeI CTNAG 5 cut(s) 182, 318, 343, 445, 1563
DpnI GATC 4 cut(s) 458, 585, 1269, 1809
DpnII GATC 4 cut(s) 456, 583, 1267, 1807
EaeI YGGCCR 1 cut(s) 543
Eam1104I CTCTTC 1 cut(s) 1683
EarI CTCTTC 1 cut(s) 1683
EciI GGCGGA 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 522
Eco147I AGGCCT 1 cut(s) 90
Eco31I GGTCTC 2 cut(s) 29, 512
Eco32I GATATC 2 cut(s) 770, 1582
Eco47III AGCGCT 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 1225
EcoNI CCTNNNNNAGG 2 cut(s) 346, 1562
EcoRI GAATTC 2 cut(s) 712, 1340
EcoRII CCWGG 3 cut(s) 260, 494, 815
EcoRV GATATC 2 cut(s) 770, 1582
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaqI GGGAC 3 cut(s) 75, 493, 797
FbaI TGATCA 2 cut(s) 583, 1267
FokI GGATG 7 cut(s) 85, 434, 1172, 1337, 1541, 1589, 1724
FspBI CTAG 7 cut(s) 39, 108, 599, 725, 1055, 1133, 1404
GlaI GCGC 3 cut(s) 37, 140, 636
GsaI CCCAGC 3 cut(s) 134, 856, 911
GsuI CTGGAG 1 cut(s) 1035
HaeII RGCGCY 2 cut(s) 39, 142
HaeIII GGCC 3 cut(s) 90, 545, 906
HapII CCGG 6 cut(s) 123, 403, 531, 546, 891, 1093
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 3 cut(s) 38, 141, 637
Hin6I GCGC 3 cut(s) 36, 139, 635
HinP1I GCGC 3 cut(s) 36, 139, 635
HincII GTYRAC 1 cut(s) 1630
HindII GTYRAC 1 cut(s) 1630
HinfI GANTC 4 cut(s) 218, 320, 443, 1044
HpaI GTTAAC 1 cut(s) 1630
HpaII CCGG 6 cut(s) 123, 403, 531, 546, 891, 1093
HphI GGTGA 4 cut(s) 463, 1342, 1482, 1553
Hpy166II GTNNAC 3 cut(s) 1237, 1544, 1630
Hpy188I TCNGA 6 cut(s) 72, 185, 319, 365, 387, 1159
Hpy188III TCNNGA 7 cut(s) 349, 531, 656, 1093, 1115, 1243, 1460
Hpy8I GTNNAC 3 cut(s) 1237, 1544, 1630
Hpy99I CGWCG 3 cut(s) 369, 372, 375
HpyAV CCTTC 4 cut(s) 180, 1364, 1466, 1618
HpyCH4III ACNGT 5 cut(s) 248, 466, 1234, 1371, 1682
HpyCH4IV ACGT 8 cut(s) 7, 64, 164, 224, 373, 837, 1600, 1695
HpyCH4V TGCA 6 cut(s) 745, 848, 878, 1607, 1615, 1796
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 299, 616, 938, 1754
HpyF3I CTNAG 5 cut(s) 182, 318, 343, 445, 1563
HpySE526I ACGT 8 cut(s) 7, 64, 164, 224, 373, 837, 1600, 1695
HspAI GCGC 3 cut(s) 36, 139, 635
Kpn2I TCCGGA 2 cut(s) 530, 1092
KroI GCCGGC 1 cut(s) 545
KroNI GCCGGC 1 cut(s) 547
Ksp22I TGATCA 2 cut(s) 583, 1267
KspAI GTTAAC 1 cut(s) 1630
Kzo9I GATC 4 cut(s) 456, 583, 1267, 1807
LmnI GCTCC 2 cut(s) 366, 405
LweI GCATC 4 cut(s) 277, 412, 865, 1150
MaeI CTAG 7 cut(s) 39, 108, 599, 725, 1055, 1133, 1404
MaeII ACGT 8 cut(s) 7, 64, 164, 224, 373, 837, 1600, 1695
MaeIII GTNAC 2 cut(s) 160, 796
MalI GATC 4 cut(s) 458, 585, 1269, 1809
MbiI CCGCTC 2 cut(s) 1255, 1441
MboI GATC 4 cut(s) 456, 583, 1267, 1807
MboII GAAGA 8 cut(s) 293, 296, 466, 1034, 1297, 1343, 1484, 1700
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 1 cut(s) 410
MluCI AATT 8 cut(s) 555, 712, 981, 1340, 1499, 1648, 1767, 1774
MlyI GAGTC 2 cut(s) 329, 437
MmeI TCCRAC 3 cut(s) 388, 745, 1021
MroI TCCGGA 2 cut(s) 530, 1092
MroNI GCCGGC 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 1083
MseI TTAA 5 cut(s) 150, 243, 1175, 1620, 1629
MslI CAYNNNNRTG 2 cut(s) 660, 1464
MspA1I CMGCKG 1 cut(s) 1304
MspI CCGG 6 cut(s) 123, 403, 531, 546, 891, 1093
MspR9I CCNGG 4 cut(s) 262, 496, 817, 891
MvaI CCWGG 3 cut(s) 262, 496, 817
MwoI GCNNNNNNNGC 5 cut(s) 296, 299, 616, 938, 1754
NaeI GCCGGC 1 cut(s) 547
NciI CCSGG 1 cut(s) 891
NcoI CCATGG 1 cut(s) 522
NdeII GATC 4 cut(s) 456, 583, 1267, 1807
NgoMIV GCCGGC 1 cut(s) 545
NlaIV GGNNCC 3 cut(s) 529, 1097, 1524
PagI TCATGA 2 cut(s) 655, 1459
PceI AGGCCT 1 cut(s) 90
PdiI GCCGGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 1083
PfeI GAWTC 2 cut(s) 218, 1044
PflMI CCANNNNNTGG 1 cut(s) 859
PfoI TCCNGGA 1 cut(s) 889
PleI GAGTC 2 cut(s) 328, 437
PpsI GAGTC 2 cut(s) 328, 437
Psp1406I AACGTT 1 cut(s) 7
Psp6I CCWGG 3 cut(s) 260, 494, 815
PspFI CCCAGC 3 cut(s) 130, 852, 907
PspGI CCWGG 3 cut(s) 260, 494, 815
PspN4I GGNNCC 3 cut(s) 529, 1097, 1524
PspPI GGNCC 1 cut(s) 905
PstI CTGCAG 1 cut(s) 1617
PstNI CAGNNNCTG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 456
PvuII CAGCTG 1 cut(s) 1304
RsaI GTAC 3 cut(s) 259, 1131, 1569
RsaNI GTAC 3 cut(s) 258, 1130, 1568
RseI CAYNNNNRTG 2 cut(s) 660, 1464
SaqAI TTAA 5 cut(s) 150, 243, 1175, 1620, 1629
Sau3AI GATC 4 cut(s) 456, 583, 1267, 1807
Sau96I GGNCC 1 cut(s) 905
SchI GAGTC 2 cut(s) 329, 437
ScrFI CCNGG 4 cut(s) 262, 496, 817, 891
SduI GDGCHC 1 cut(s) 410
SfaNI GCATC 4 cut(s) 277, 412, 865, 1150
SfcI CTRYAG 5 cut(s) 247, 1215, 1305, 1613, 1681
SmiMI CAYNNNNRTG 2 cut(s) 660, 1464
SmlI CTYRAG 2 cut(s) 719, 774
SmoI CTYRAG 2 cut(s) 719, 774
SpeI ACTAGT 1 cut(s) 1132
Sse9I AATT 8 cut(s) 555, 712, 981, 1340, 1499, 1648, 1767, 1774
SseBI AGGCCT 1 cut(s) 90
SsiI CCGC 6 cut(s) 33, 168, 190, 266, 1255, 1439
SspI AATATT 1 cut(s) 970
SspMI CTAG 7 cut(s) 39, 108, 599, 725, 1055, 1133, 1404
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 4 cut(s) 260, 494, 815, 889
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 5 cut(s) 248, 466, 1234, 1371, 1682
TaiI ACGT 8 cut(s) 10, 67, 167, 227, 376, 840, 1603, 1698
TaqI TCGA 2 cut(s) 1392, 1674
TaqII GACCGA 1 cut(s) 319
TasI AATT 8 cut(s) 555, 712, 981, 1340, 1499, 1648, 1767, 1774
TauI GCSGC 3 cut(s) 36, 193, 269
TfiI GAWTC 2 cut(s) 218, 1044
Tru1I TTAA 5 cut(s) 150, 243, 1175, 1620, 1629
Tru9I TTAA 5 cut(s) 150, 243, 1175, 1620, 1629
TscAI CASTG 1 cut(s) 453
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 1 cut(s) 453
Van91I CCANNNNNTGG 1 cut(s) 859
XagI CCTNNNNNAGG 2 cut(s) 346, 1562
XapI RAATTY 5 cut(s) 555, 712, 1340, 1648, 1767
XcmI CCANNNNNNNNNTGG 1 cut(s) 953
XmnI GAANNNNTTC 1 cut(s) 1083
XspI CTAG 7 cut(s) 39, 108, 599, 725, 1055, 1133, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.