pycom08g00010

Protease Do-like 10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014559
Physical Location & Seq
Reverse (-)
4009 .. 9214
5206 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00010.1

Sequence Viewer

Length: 1794 bp
ATGAAATACTACGCCATCGTTTATTTCTTGTTTCAGAAATCAGAGCGACTGCCGTGCAAAATACAAAAAGACATGCTGGGAACGTCCGTCCATAGACTCACCAGGCTCTCTGTTTCCTCTATAAAGTTAATAGCTAGTAAGGCAACCGCAACCGCAAGAGGGACTAGTACTTGTACTTCTACCATTACCAGGAAAGCCGCAGCTACTGTTGAAGAAGTTGCGGCAGCCGCCGCCGCCGCGTCTTCTTCTTGTTCCTCCGAGCCACTTAACTCTAATGGGCCTTTCCCCTACCCCTCATCAGCTGCAGCGCACCTGCAACGACGACGCCCACGACCACGTTTAGGTCTAGGTGATGGGAGGAACATCCAGAGGAGAAGCACGACGGATACGGATGCAATAGAGCTAGCATTGGATTCAGTGGTGAAGATCTTCACTGTGACGAGCAGCCCTAACTACTTCCTTCCTTGGCAGAACAAGTCCCAGCGAGAAACCATGGGCTCCGGGTTCGTAATCCGTGGGAAGAAAATTCTGACAAATGCTCATGTAGTGGCTGATCATACCTTTGTACTTGTAAGAAAGCATGGTTCTCCTACCAAGTACAGAGCGCAAGTTAACGCTGTTGGTCATGAATGTGATTTAGCCATTTTGACTGTTGAAAGTCAAGACTTCTGGGAGGGTATGACTTCCCTCGATCTAGGGGACATTCCTTTTTTGCAACAAGCCGTGGCTGTTGTTGGATATCCTCAAGGTGGTGATAACATTTCAGTTACCAAAGGTGTTGTTTCTAGGGTTGAACCCACTCAATATGTACACGGGGCAACCCAGCTCATGGCAATACAAATTGATGCTGCAATAAATCCAGGGAATAGTGGCGGTCCAGCAATCATGGGCAATAAAGTTGCTGGTGTGGCTTTCCAGAACCTTTCTGGTGCAGAGAATATTGGTTATATAATTCCTGTCCCAGTAATAAAGCATTTTATTGCTGGAGTGGAAGAAAGTGGCAAGTATGTTGGATTCTGCTCTCTGGGTTTGTCATGCCAACCCATTGAAAATGTTCAACTGAGGAACCACTTTCGGATGCGCCCTGAAATGACCGGGGTACTAGTGAGCAAAATTAACCCTCTCTCGGATGCCTATAAAGTCTTGAAGAAAGATGATATTATCCTTGTTTTTGATGGAGTGCCTGTAGCGAATGATGGAACAGTTCATTTTCGAAACAGAGAGCGGATAACATTTGACCATTTGGTTTCTATGAAGAAACCCAATGAAACAGCCATAGTTAGAGTTCTGAGGGACGGTGAAGAGTATGAATTCAGTATCGCCCTTCAACCTTTACAGCCATTAGTTCCAGTTCATCAGTTTGATAAGCTTCCTAGTTATTACATTTTTGCTGGTTTGGTCTTTGTTCCACTCACTCAGCCATACCTTCACGAGTATGGAGAAGACTGGTACAATACTTCACCTCGACGATTGTGTGAAAGCGCATTGAGGGAACCACCAAAACGGCCTGGTCAACAGCTTGTCATCCTTTCTCAGGTCCTAATGGACGATATCAATGCAGGATATGAACGTCTTGCAGAGTTACAGGTTAAGAAGGTTAACGGAGTTGAAGTTGACAATTTGAAACATTTATGCCGACTTGTCGAAAACTGCAGCGAAGAGAGTGTGCGCTTTGATTTGGATGATGATAGGGTTATTGTGTTAAACCGTCGCTTAGCAAAAGTTGCAACTTCAAGGATTTTGAAGTGCCATCGAATACCCTCAGTAATGTCTGATGATCTTACCATCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

598

Amino Acids

65.9

Weight (kDa)

8.82

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trypsin PF00089 149 - 327 6.1e-18 Trypsin
Trypsin_2 PF13365 166 - 295 5.2e-21 Trypsin-like peptidase domain
PDZ_3 PF17815 449 - 593 1.1e-56 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014559)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36950
fragaria_vesca FvH4_3g00010
malus_domestica MD08G1000100.v1.1
prunus_persica Prupe.4G000300_v2.0.a1
pyrus_communis pycom08g00010
rosa_chinensis RchiOBHm_Chr5g0000001
rosa_laevigata RLG00000030831
rosa_multiflora Rmu_sc0007644.1_g000006
rosa_roxburghii Rroxscaffold_1G00076060 Rroxscaffold_2G00109790 Rroxscaffold_4G00330340
rosa_rugosa Rorug04G0380000
rosa_samantha Rh5AG000100 Rh5BG001300 Rh5CG000100 Rh5DG000100
rosa_wichuraiana Rw5G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 321
Acc36I ACCTGC 1 cut(s) 321
AccB7I CCANNNNNTGG 1 cut(s) 829
AccBSI CCGCTC 1 cut(s) 1225
AccII CGCG 1 cut(s) 239
AcsI RAATTY 2 cut(s) 525, 1310
AcyI GRCGYC 1 cut(s) 325
AfaI GTAC 7 cut(s) 169, 175, 567, 599, 810, 1101, 1451
AfiI CCNNNNNNNGG 7 cut(s) 159, 189, 341, 749, 829, 1126, 1534
AhlI ACTAGT 2 cut(s) 164, 1102
AjnI CCWGG 4 cut(s) 101, 188, 859, 1507
AluBI AGCT 7 cut(s) 134, 203, 302, 403, 826, 1369, 1519
AluI AGCT 7 cut(s) 134, 203, 302, 403, 826, 1369, 1519
AlwNI CAGNNNCTG 1 cut(s) 206
AoxI GGCC 2 cut(s) 278, 1505
ApeKI GCWGC 7 cut(s) 200, 224, 302, 305, 444, 848, 1653
ApoI RAATTY 2 cut(s) 525, 1310
Asp700I GAANNNNTTC 2 cut(s) 428, 1053
AspLEI GCGC 5 cut(s) 310, 607, 1083, 1484, 1671
AspS9I GGNCC 3 cut(s) 278, 875, 1537
AsuC2I CCSGG 2 cut(s) 502, 1096
AsuHPI GGTGA 6 cut(s) 91, 362, 433, 764, 1310, 1452
AsuII TTCGAA 1 cut(s) 1213
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 2 cut(s) 875, 1537
BanII GRGCYC 1 cut(s) 500
BarI GAAGNNNNNNTAC 2 cut(s) 160, 192
BauI CACGAG 1 cut(s) 1430
BbsI GAAGAC 2 cut(s) 234, 1449
BbvI GCAGC 7 cut(s) 212, 236, 289, 317, 456, 835, 1665
BccI CCATC 6 cut(s) 23, 347, 1169, 1190, 1758, 1793
BceAI ACGGC 3 cut(s) 37, 707, 1520
BcgI CGANNNNNNTGC 4 cut(s) 36, 70, 1538, 1572
BciT130I CCWGG 4 cut(s) 103, 190, 861, 1509
BciVI GTATCC 1 cut(s) 379
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 2 cut(s) 502, 1096
BcuI ACTAGT 2 cut(s) 164, 1102
BfaI CTAG 8 cut(s) 135, 165, 347, 404, 695, 786, 1103, 1374
BfmI CTRYAG 3 cut(s) 303, 1185, 1651
BfuAI ACCTGC 1 cut(s) 321
BfuI GTATCC 1 cut(s) 379
BglII AGATCT 1 cut(s) 426
BlpI GCTNAGC 1 cut(s) 1714
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 6 cut(s) 103, 190, 502, 861, 1096, 1509
Bme18I GGWCC 2 cut(s) 875, 1537
BmgT120I GGNCC 3 cut(s) 278, 875, 1537
BmiI GGNNCC 3 cut(s) 499, 1067, 1494
BmrFI CCNGG 6 cut(s) 103, 190, 502, 861, 1096, 1509
BmrI ACTGGG 1 cut(s) 956
BmsI GCATC 4 cut(s) 382, 835, 1068, 1120
BmtI GCTAGC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 956
BpiI GAAGAC 2 cut(s) 234, 1449
BpmI CTGGAG 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 1714
Bpu14I TTCGAA 1 cut(s) 1213
BpuEI CTTGAG 1 cut(s) 729
BpuMI CCSGG 2 cut(s) 502, 1096
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 6 cut(s) 464, 492, 514, 723, 860, 1095
BsaXI ACNNNNNCTCC 4 cut(s) 1170, 1200, 1596, 1626
Bsc4I CCNNNNNNNGG 7 cut(s) 159, 189, 341, 749, 829, 1126, 1534
Bse1I ACTGG 3 cut(s) 962, 1349, 1451
BseBI CCWGG 4 cut(s) 103, 190, 861, 1509
BseDI CCNNGG 6 cut(s) 464, 492, 514, 723, 860, 1095
BseGI GGATG 6 cut(s) 363, 397, 1083, 1135, 1524, 1687
BseLI CCNNNNNNNGG 7 cut(s) 159, 189, 341, 749, 829, 1126, 1534
BseMII CTCAG 5 cut(s) 1052, 1280, 1430, 1547, 1776
BseNI ACTGG 3 cut(s) 962, 1349, 1451
BseRI GAGGAG 1 cut(s) 385
BseXI GCAGC 7 cut(s) 212, 236, 289, 317, 456, 835, 1665
BseYI CCCAGC 3 cut(s) 76, 480, 822
BsgI GTGCAG 1 cut(s) 951
Bsh1236I CGCG 1 cut(s) 239
BshFI GGCC 2 cut(s) 280, 1507
BsiSI CCGG 2 cut(s) 501, 1095
BslFI GGGAC 5 cut(s) 175, 463, 713, 944, 1307
BslI CCNNNNNNNGG 7 cut(s) 159, 189, 341, 749, 829, 1126, 1534
BsmFI GGGAC 5 cut(s) 175, 463, 713, 944, 1307
BsnI GGCC 2 cut(s) 280, 1507
Bsp119I TTCGAA 1 cut(s) 1213
Bsp1286I GDGCHC 1 cut(s) 500
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 4 cut(s) 426, 553, 691, 1777
Bsp1720I GCTNAGC 1 cut(s) 1714
Bsp19I CCATGG 1 cut(s) 492
BspANI GGCC 2 cut(s) 280, 1507
BspCNI CTCAG 5 cut(s) 1053, 1281, 1429, 1546, 1775
BspFNI CGCG 1 cut(s) 239
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 3 cut(s) 499, 1067, 1494
BspMAI CTGCAG 2 cut(s) 307, 1655
BspMI ACCTGC 1 cut(s) 321
BspOI GCTAGC 1 cut(s) 407
BspT104I TTCGAA 1 cut(s) 1213
BsrBI CCGCTC 1 cut(s) 1225
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 3 cut(s) 962, 1349, 1451
BssECI CCNNGG 6 cut(s) 464, 492, 514, 723, 860, 1095
BssMI GATC 4 cut(s) 426, 553, 691, 1777
BssNI GRCGYC 1 cut(s) 325
BssSI CACGAG 1 cut(s) 1430
BssT1I CCWWGG 2 cut(s) 464, 492
Bst2BI CACGAG 1 cut(s) 1430
Bst2UI CCWGG 4 cut(s) 103, 190, 861, 1509
Bst4CI ACNGT 6 cut(s) 208, 436, 652, 1204, 1298, 1709
Bst6I CTCTTC 2 cut(s) 1296, 1653
BstACI GRCGYC 1 cut(s) 325
BstAPI GCANNNNNTGC 1 cut(s) 1724
BstAUI TGTACA 1 cut(s) 808
BstBI TTCGAA 1 cut(s) 1213
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 7 cut(s) 1061, 1289, 1416, 1533, 1714, 1762, 1791
BstDSI CCRYGG 3 cut(s) 492, 514, 723
BstENI CCTNNNNNAGG 1 cut(s) 1532
BstF5I GGATG 6 cut(s) 363, 397, 1083, 1135, 1524, 1687
BstFNI CGCG 1 cut(s) 239
BstHHI GCGC 5 cut(s) 310, 607, 1083, 1484, 1671
BstKTI GATC 4 cut(s) 429, 556, 694, 1780
BstMBI GATC 4 cut(s) 426, 553, 691, 1777
BstMWI GCNNNNNNNGC 7 cut(s) 140, 227, 230, 233, 236, 908, 1724
BstNI CCWGG 4 cut(s) 103, 190, 861, 1509
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 6 cut(s) 101, 188, 500, 859, 1094, 1507
BstSFI CTRYAG 3 cut(s) 303, 1185, 1651
BstUI CGCG 1 cut(s) 239
BstV1I GCAGC 7 cut(s) 212, 236, 289, 317, 456, 835, 1665
BstV2I GAAGAC 2 cut(s) 234, 1449
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 2 cut(s) 280, 1507
BtgI CCRYGG 3 cut(s) 492, 514, 723
BtsCI GGATG 6 cut(s) 363, 397, 1083, 1135, 1524, 1687
BtsIMutI CAGTG 2 cut(s) 423, 432
BveI ACCTGC 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 405
CaiI CAGNNNCTG 1 cut(s) 206
CciI TCATGA 1 cut(s) 625
CfoI GCGC 5 cut(s) 310, 607, 1083, 1484, 1671
Cfr13I GGNCC 3 cut(s) 278, 875, 1537
CseI GACGC 2 cut(s) 228, 333
Csp6I GTAC 7 cut(s) 168, 174, 566, 598, 809, 1100, 1450
CviAII CATG 8 cut(s) 73, 493, 542, 581, 626, 829, 886, 1035
CviQI GTAC 7 cut(s) 168, 174, 566, 598, 809, 1100, 1450
DdeI CTNAG 7 cut(s) 1061, 1289, 1416, 1533, 1714, 1762, 1791
DpnI GATC 4 cut(s) 428, 555, 693, 1779
DpnII GATC 4 cut(s) 426, 553, 691, 1777
Eam1104I CTCTTC 2 cut(s) 1296, 1653
EarI CTCTTC 2 cut(s) 1296, 1653
Eco130I CCWWGG 2 cut(s) 464, 492
Eco24I GRGCYC 1 cut(s) 500
Eco32I GATATC 2 cut(s) 740, 1552
Eco47I GGWCC 2 cut(s) 875, 1537
EcoNI CCTNNNNNAGG 1 cut(s) 1532
EcoO109I RGGNCCY 1 cut(s) 1537
EcoRI GAATTC 1 cut(s) 1310
EcoRII CCWGG 4 cut(s) 101, 188, 859, 1507
EcoRV GATATC 2 cut(s) 740, 1552
EcoT14I CCWWGG 2 cut(s) 464, 492
EcoT38I GRGCYC 1 cut(s) 500
ErhI CCWWGG 2 cut(s) 464, 492
FaeI CATG 8 cut(s) 76, 496, 545, 584, 629, 832, 889, 1038
FaqI GGGAC 5 cut(s) 175, 463, 713, 944, 1307
FatI CATG 8 cut(s) 72, 492, 541, 580, 625, 828, 885, 1034
FbaI TGATCA 1 cut(s) 553
FokI GGATG 6 cut(s) 350, 404, 1090, 1142, 1511, 1694
FriOI GRGCYC 1 cut(s) 500
FspBI CTAG 8 cut(s) 135, 165, 347, 404, 695, 786, 1103, 1374
GlaI GCGC 5 cut(s) 309, 606, 1082, 1483, 1670
GsaI CCCAGC 3 cut(s) 80, 484, 826
GsuI CTGGAG 1 cut(s) 1005
HaeIII GGCC 2 cut(s) 280, 1507
HapII CCGG 2 cut(s) 501, 1095
HgaI GACGC 2 cut(s) 228, 333
HhaI GCGC 5 cut(s) 310, 607, 1083, 1484, 1671
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 8 cut(s) 76, 496, 545, 584, 629, 832, 889, 1038
Hin6I GCGC 5 cut(s) 308, 605, 1081, 1482, 1669
HinP1I GCGC 5 cut(s) 308, 605, 1081, 1482, 1669
HincII GTYRAC 4 cut(s) 613, 1514, 1600, 1615
HindII GTYRAC 4 cut(s) 613, 1514, 1600, 1615
HindIII AAGCTT 1 cut(s) 1367
HinfI GANTC 3 cut(s) 96, 413, 1014
HpaI GTTAAC 2 cut(s) 613, 1600
HpaII CCGG 2 cut(s) 501, 1095
HphI GGTGA 6 cut(s) 91, 362, 433, 764, 1310, 1452
Hpy166II GTNNAC 5 cut(s) 613, 811, 1514, 1600, 1615
Hpy188I TCNGA 8 cut(s) 36, 43, 259, 531, 1077, 1129, 1290, 1774
Hpy188III TCNNGA 6 cut(s) 367, 626, 662, 916, 1144, 1430
Hpy8I GTNNAC 5 cut(s) 613, 811, 1514, 1600, 1615
Hpy99I CGWCG 5 cut(s) 324, 327, 385, 1470, 1713
HpyAV CCTTC 4 cut(s) 470, 1334, 1436, 1588
HpyCH4III ACNGT 6 cut(s) 208, 436, 652, 1204, 1298, 1709
HpyCH4IV ACGT 3 cut(s) 83, 337, 1570
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 227, 230, 233, 236, 908, 1724
HpyF3I CTNAG 7 cut(s) 1061, 1289, 1416, 1533, 1714, 1762, 1791
HpySE526I ACGT 3 cut(s) 83, 337, 1570
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 8 cut(s) 76, 496, 545, 584, 629, 832, 889, 1038
HspAI GCGC 5 cut(s) 308, 605, 1081, 1482, 1669
Ksp22I TGATCA 1 cut(s) 553
KspAI GTTAAC 2 cut(s) 613, 1600
Kzo9I GATC 4 cut(s) 426, 553, 691, 1777
LmnI GCTCC 1 cut(s) 503
Lsp1109I GCAGC 7 cut(s) 212, 236, 289, 317, 456, 835, 1665
LweI GCATC 4 cut(s) 382, 835, 1068, 1120
MaeI CTAG 8 cut(s) 135, 165, 347, 404, 695, 786, 1103, 1374
MaeII ACGT 3 cut(s) 83, 337, 1570
MaeIII GTNAC 3 cut(s) 436, 766, 1581
MalI GATC 4 cut(s) 428, 555, 693, 1779
MbiI CCGCTC 1 cut(s) 1225
MboI GATC 4 cut(s) 426, 553, 691, 1777
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 500
MluCI AATT 6 cut(s) 525, 840, 951, 1113, 1310, 1618
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 2 cut(s) 715, 991
MroXI GAANNNNTTC 2 cut(s) 428, 1053
MseI TTAA 7 cut(s) 128, 267, 612, 1116, 1590, 1599, 1703
MslI CAYNNNNRTG 2 cut(s) 630, 1434
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 2 cut(s) 501, 1095
MspR9I CCNGG 6 cut(s) 103, 190, 502, 861, 1096, 1509
MvaI CCWGG 4 cut(s) 103, 190, 861, 1509
MvnI CGCG 1 cut(s) 239
MwoI GCNNNNNNNGC 7 cut(s) 140, 227, 230, 233, 236, 908, 1724
NciI CCSGG 2 cut(s) 502, 1096
NcoI CCATGG 1 cut(s) 492
NdeII GATC 4 cut(s) 426, 553, 691, 1777
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 8 cut(s) 76, 496, 545, 584, 629, 832, 889, 1038
NlaIV GGNNCC 3 cut(s) 499, 1067, 1494
NmuCI GTSAC 1 cut(s) 436
NspI RCATGY 1 cut(s) 76
NspV TTCGAA 1 cut(s) 1213
PagI TCATGA 1 cut(s) 625
PaqCI CACCTGC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 328
PdmI GAANNNNTTC 2 cut(s) 428, 1053
PfeI GAWTC 2 cut(s) 413, 1014
PflMI CCANNNNNTGG 1 cut(s) 829
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
PpuMI RGGWCCY 1 cut(s) 1537
Psp5II RGGWCCY 1 cut(s) 1537
Psp6I CCWGG 4 cut(s) 101, 188, 859, 1507
PspFI CCCAGC 3 cut(s) 76, 480, 822
PspGI CCWGG 4 cut(s) 101, 188, 859, 1507
PspN4I GGNNCC 3 cut(s) 499, 1067, 1494
PspPI GGNCC 3 cut(s) 278, 875, 1537
PspPPI RGGWCCY 1 cut(s) 1537
PstI CTGCAG 2 cut(s) 307, 1655
PstNI CAGNNNCTG 1 cut(s) 206
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 7 cut(s) 169, 175, 567, 599, 810, 1101, 1451
RsaNI GTAC 7 cut(s) 168, 174, 566, 598, 809, 1100, 1450
RseI CAYNNNNRTG 2 cut(s) 630, 1434
SaqAI TTAA 7 cut(s) 128, 267, 612, 1116, 1590, 1599, 1703
Sau3AI GATC 4 cut(s) 426, 553, 691, 1777
Sau96I GGNCC 3 cut(s) 278, 875, 1537
ScaI AGTACT 1 cut(s) 169
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 6 cut(s) 103, 190, 502, 861, 1096, 1509
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 4 cut(s) 382, 835, 1068, 1120
SfcI CTRYAG 3 cut(s) 303, 1185, 1651
SfuI TTCGAA 1 cut(s) 1213
SinI GGWCC 2 cut(s) 875, 1537
SmiMI CAYNNNNRTG 2 cut(s) 630, 1434
SmlI CTYRAG 1 cut(s) 744
SmoI CTYRAG 1 cut(s) 744
SpeI ACTAGT 2 cut(s) 164, 1102
Sse9I AATT 6 cut(s) 525, 840, 951, 1113, 1310, 1618
SspI AATATT 1 cut(s) 940
SspMI CTAG 8 cut(s) 135, 165, 347, 404, 695, 786, 1103, 1374
StyD4I CCNGG 6 cut(s) 101, 188, 500, 859, 1094, 1507
StyI CCWWGG 2 cut(s) 464, 492
TaaI ACNGT 6 cut(s) 208, 436, 652, 1204, 1298, 1709
TaiI ACGT 3 cut(s) 86, 340, 1573
TaqI TCGA 5 cut(s) 690, 1213, 1465, 1644, 1753
TasI AATT 6 cut(s) 525, 840, 951, 1113, 1310, 1618
TatI WGTACW 5 cut(s) 167, 173, 565, 597, 808
TauI GCSGC 6 cut(s) 200, 224, 230, 233, 236, 239
TfiI GAWTC 2 cut(s) 413, 1014
Tru1I TTAA 7 cut(s) 128, 267, 612, 1116, 1590, 1599, 1703
Tru9I TTAA 7 cut(s) 128, 267, 612, 1116, 1590, 1599, 1703
TscAI CASTG 2 cut(s) 423, 439
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 7 cut(s) 200, 224, 302, 305, 444, 848, 1653
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 8 cut(s) 17, 642, 1196, 1268, 1281, 1323, 1343, 1581
TspGWI ACGGA 5 cut(s) 76, 398, 404, 503, 1617
TspRI CASTG 2 cut(s) 423, 439
Van91I CCANNNNNTGG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 875, 1537
XagI CCTNNNNNAGG 1 cut(s) 1532
XapI RAATTY 2 cut(s) 525, 1310
XceI RCATGY 1 cut(s) 76
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XmnI GAANNNNTTC 2 cut(s) 428, 1053
XspI CTAG 8 cut(s) 135, 165, 347, 404, 695, 786, 1103, 1374
ZrmI AGTACT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.