Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0007339

Physical Location & Seq

Genomic Coordinates Reverse (-)

933825 .. 937208

Gene Structure 3384 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g01820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1227 bp

ATGAGAGGAGGGGGTTTGTGGCAGCTTGGTCAGTCAATCACCCGACGTCTTGCTCAGGGTGATAAGAAGGGTGTGGCTCGTCGCTACTTTGCTTCAGAAGCTGAGCTCAAAAAGACAGTTCTTTATGACTTCCATGTCGCTAATGGTGGGAAGATGGTGCCGTTTGCTGGATGGGGCATGCCTATTCAGTATAAGGACTCGATTATGGACTCCACTGTGAACTGTAGGGAGAACGGTGGGCTTTTTGATGTCTCTCATATGTGTGGCCTGAGTCTCAAGGGGAAGGACTCCATTCCGTTCCTTGAGAAGCTGGTCATTGCTGATGTTGCTGGTCTGGCCCCTGGAACGGGGACACTAACTGTCTTTACAAATGAGAAGGGAGGGGCAATTGATGACTCTGTTGTCACCAAGGTCACTGATGACCACATATACTTGGTGGTGAATGCTGGATGTAGGGATAAGGATTTGGCTCACATTGAGGAGCACATGAAGGCATTCAAGGCCAAAGGAGGAGACGTCTCATGGCACATTCATGATGAGAGATCTCTTCTAGCTCTTCAGGGTCCTCTTGCTGCCCCAACTCTTCAACACTTGACAAAAGAAGATTTGAGCAAGTTTTATTTCGGAGAGTTCCGAATTTTGGATATCAATGGGGCACACTGCTTTCTCACTAGGACTGGGTACACTGGTGAAGATGGATTTGAAATCTCAGTTCCTGATGAGAATGCATTGGATCTTGCCAAAGCAATCTTGGAGAAATCTGAGGGGAAGGTGAGACTGACTGGATTGGGAGCTCGTGATAGTCTCCGATTGGAAGCTGGTTTGTGTTTATATGGTAATGATATGGAGCAACACATAACACCAGTGGAGGCTGGGCTCACATGGGCTATAGGCAAGAGAAGAAGGGCAGAAGGTGGCTTTCTTGGAGCTGAGGTGATTCTCAAACAACTTGCTGATGGTCCTTCGATCAGGCGTGCTGGTTTCATCTCTTCAGGCCCTCCTGCTAGAGGTCACAGTGAGATTCAGAATGAGAAAGGTGAAACCATTGGGGAAGTGACAAGTGGAGGATTCAGCCCTTGCCTGAAGAAAAATATTGCTATGGGGTACGTGAAATCTGGAAACCACAAGGCTGGAACAAAAGTTAAGATTGTTGTGCGAGGAAAGGCCTATGATGGAACTGTCACTAAAATGCCTTTTGTACCAACAAAATACTACAAGCCAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005840 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009941 GO:0010035 GO:0010038 GO:0016020 GO:0022626 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046686 GO:0048046 GO:0050896 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko00260 ko00630 ko00670 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map00670 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

GCV_T GCV_T_C

Protein Analysis

409

Amino Acids

44.2

Weight (kDa)

8.69

Isoelectric Point (pI)

40.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GCV_T	PF01571	41 - 299	3.7e-73	GCVT N-terminal domain
GCV_T_C	PF08669	327 - 400	3.5e-19	Glycine cleavage T-protein C-terminal barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007339)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11860 AT1G11860 AT1G11860
fragaria_vesca	FvH4_3g01820 FvH4_3g01820 FvH4_3g01820
malus_domestica	MD05G1348800.v1.1 MD10G1323700.v1.1
prunus_persica	Prupe.4G018500_v2.0.a1 Prupe.4G018500_v2.0.a1 Prupe.4G018500_v2.0.a1
pyrus_communis	pycom05g31640 pycom10g27410
rosa_chinensis	RchiOBHm_Chr5g0002651
rosa_laevigata	RLG00000031050
rosa_multiflora	Rmu_co8214212.1_g000001 Rmu_sc0009084.1_g000015
rosa_roxburghii	Rroxscaffold_1G00073210
rosa_rugosa	Rorug04G0398100 Rorug04G0398200
rosa_samantha	Rh5AG021500 Rh5BG024200 Rh5CG023700 Rh5DG022600
rosa_wichuraiana	Rw5G001990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	134, 401
AatII	GACGTC	2 cut(s)	49, 519
AccB1I	GGYRCC	1 cut(s)	157
AclWI	GGATC	1 cut(s)	741
AcsI	RAATTY	1 cut(s)	636
AcuI	CTGAAG	4 cut(s)	78, 542, 975, 1103
AcyI	GRCGYC	2 cut(s)	46, 516
AfaI	GTAC	3 cut(s)	683, 1106, 1200
AfiI	CCNNNNNNNGG	5 cut(s)	167, 346, 347, 640, 1007
AgsI	TTSAA	3 cut(s)	499, 587, 704
AjnI	CCWGG	1 cut(s)	340
AluBI	AGCT	8 cut(s)	25, 101, 106, 310, 554, 794, 818, 929
AluI	AGCT	8 cut(s)	25, 101, 106, 310, 554, 794, 818, 929
Alw21I	GWGCWC	3 cut(s)	108, 486, 796
Alw26I	GTCTC	6 cut(s)	256, 278, 507, 523, 769, 809
AlwI	GGATC	1 cut(s)	741
AlwNI	CAGNNNCTG	2 cut(s)	101, 716
AoxI	GGCC	5 cut(s)	265, 336, 501, 994, 1164
ApeKI	GCWGC	2 cut(s)	22, 572
ApoI	RAATTY	1 cut(s)	636
Asp700I	GAANNNNTTC	1 cut(s)	494
AspS9I	GGNCC	4 cut(s)	337, 563, 959, 995
AsuHPI	GGTGA	8 cut(s)	31, 71, 397, 451, 701, 784, 946, 1049
AvaII	GGWCC	2 cut(s)	563, 959
BaeGI	GKGCMC	1 cut(s)	658
BaeI	ACNNNNGTAYC	2 cut(s)	673, 706
BanI	GGYRCC	1 cut(s)	157
BanII	GRGCYC	3 cut(s)	108, 796, 879
BauI	CACGAG	1 cut(s)	795
Bbv12I	GWGCWC	3 cut(s)	108, 486, 796
BbvCI	CCTCAGC	1 cut(s)	930
BbvI	GCAGC	2 cut(s)	34, 559
BccI	CCATC	5 cut(s)	148, 165, 689, 950, 1166
BceAI	ACGGC	1 cut(s)	145
BciT130I	CCWGG	1 cut(s)	342
BcoDI	GTCTC	6 cut(s)	256, 278, 507, 523, 769, 809
BfaI	CTAG	3 cut(s)	551, 672, 1005
BfmI	CTRYAG	2 cut(s)	223, 888
BglII	AGATCT	1 cut(s)	542
BisI	GCNGC	2 cut(s)	23, 573
BlpI	GCTNAGC	1 cut(s)	102
BlsI	GCNGC	2 cut(s)	24, 574
Bme1390I	CCNGG	1 cut(s)	342
Bme18I	GGWCC	2 cut(s)	563, 959
BmgT120I	GGNCC	4 cut(s)	337, 563, 959, 995
BmiI	GGNNCC	3 cut(s)	159, 339, 564
BmrFI	CCNGG	1 cut(s)	342
BmrI	ACTGGG	1 cut(s)	687
BmuI	ACTGGG	1 cut(s)	687
Bpu10I	CCTNAGC	2 cut(s)	54, 930
Bpu1102I	GCTNAGC	1 cut(s)	102
BpuEI	CTTGAG	2 cut(s)	260, 323
BsaAI	YACGTR	1 cut(s)	1108
BsaHI	GRCGYC	2 cut(s)	46, 516
BsaJI	CCNNGG	2 cut(s)	340, 408
BsaXI	ACNNNNNCTCC	4 cut(s)	501, 531, 918, 948
Bsc4I	CCNNNNNNNGG	5 cut(s)	167, 346, 347, 640, 1007
Bse1I	ACTGG	4 cut(s)	682, 691, 787, 863
Bse3DI	GCAATG	1 cut(s)	315
BseBI	CCWGG	1 cut(s)	342
BseDI	CCNNGG	2 cut(s)	340, 408
BseGI	GGATG	2 cut(s)	176, 455
BseLI	CCNNNNNNNGG	5 cut(s)	167, 346, 347, 640, 1007
BseMI	GCAATG	1 cut(s)	315
BseMII	CTCAG	6 cut(s)	68, 93, 260, 723, 753, 921
BseNI	ACTGG	4 cut(s)	682, 691, 787, 863
BseRI	GAGGAG	3 cut(s)	21, 494, 525
BseSI	GKGCMC	1 cut(s)	658
BseXI	GCAGC	2 cut(s)	34, 559
BseYI	CCCAGC	1 cut(s)	872
BshFI	GGCC	5 cut(s)	267, 338, 503, 996, 1166
BshNI	GGYRCC	1 cut(s)	157
BsiHKAI	GWGCWC	3 cut(s)	108, 486, 796
BslFI	GGGAC	1 cut(s)	364
BslI	CCNNNNNNNGG	5 cut(s)	167, 346, 347, 640, 1007
BsmAI	GTCTC	6 cut(s)	256, 278, 507, 523, 769, 809
BsmBI	CGTCTC	2 cut(s)	507, 523
BsmFI	GGGAC	1 cut(s)	364
BsmI	GAATGC	3 cut(s)	448, 494, 730
BsnI	GGCC	5 cut(s)	267, 338, 503, 996, 1166
Bsp1286I	GDGCHC	5 cut(s)	108, 486, 658, 796, 879
Bsp143I	GATC	3 cut(s)	542, 733, 966
Bsp1720I	GCTNAGC	1 cut(s)	102
BspANI	GGCC	5 cut(s)	267, 338, 503, 996, 1166
BspCNI	CTCAG	6 cut(s)	67, 94, 261, 722, 754, 922
BspHI	TCATGA	1 cut(s)	532
BspLI	GGNNCC	3 cut(s)	159, 339, 564
BspPI	GGATC	1 cut(s)	741
BspQI	GCTCTTC	1 cut(s)	561
BspT107I	GGYRCC	1 cut(s)	157
BsrDI	GCAATG	1 cut(s)	315
BsrI	ACTGG	4 cut(s)	682, 691, 787, 863
BssECI	CCNNGG	2 cut(s)	340, 408
BssMI	GATC	3 cut(s)	542, 733, 966
BssNI	GRCGYC	2 cut(s)	46, 516
BssSI	CACGAG	1 cut(s)	795
BssT1I	CCWWGG	1 cut(s)	408
Bst2BI	CACGAG	1 cut(s)	795
Bst2UI	CCWGG	1 cut(s)	342
Bst4CI	ACNGT	7 cut(s)	118, 217, 224, 236, 361, 1016, 1180
Bst6I	CTCTTC	4 cut(s)	552, 561, 588, 994
BstACI	GRCGYC	2 cut(s)	46, 516
BstBAI	YACGTR	1 cut(s)	1108
BstC8I	GCNNGC	2 cut(s)	179, 975
BstDEI	CTNAG	6 cut(s)	54, 102, 269, 709, 762, 930
BstENI	CCTNNNNNAGG	1 cut(s)	1005
BstF5I	GGATG	2 cut(s)	176, 455
BstKTI	GATC	3 cut(s)	545, 736, 969
BstMAI	GTCTC	6 cut(s)	256, 278, 507, 523, 769, 809
BstMBI	GATC	3 cut(s)	542, 733, 966
BstMWI	GCNNNNNNNGC	4 cut(s)	98, 326, 335, 500
BstNI	CCWGG	1 cut(s)	342
BstNSI	RCATGY	1 cut(s)	181
BstSCI	CCNGG	1 cut(s)	340
BstSFI	CTRYAG	2 cut(s)	223, 888
BstSLI	GKGCMC	1 cut(s)	658
BstV1I	GCAGC	2 cut(s)	34, 559
BstX2I	RGATCY	2 cut(s)	542, 733
BstXI	CCANNNNNNTGG	1 cut(s)	1130
BstYI	RGATCY	2 cut(s)	542, 733
BsuRI	GGCC	5 cut(s)	267, 338, 503, 996, 1166
BtsCI	GGATG	2 cut(s)	176, 455
BtsI	GCAGTG	1 cut(s)	658
BtsIMutI	CAGTG	6 cut(s)	213, 414, 658, 684, 870, 1021
Cac8I	GCNNGC	2 cut(s)	179, 975
CaiI	CAGNNNCTG	2 cut(s)	101, 716
CciI	TCATGA	1 cut(s)	532
Cfr13I	GGNCC	4 cut(s)	337, 563, 959, 995
Csp6I	GTAC	3 cut(s)	682, 1105, 1199
CviAII	CATG	6 cut(s)	134, 178, 487, 522, 533, 882
CviQI	GTAC	3 cut(s)	682, 1105, 1199
DdeI	CTNAG	6 cut(s)	54, 102, 269, 709, 762, 930
DpnI	GATC	3 cut(s)	544, 735, 968
DpnII	GATC	3 cut(s)	542, 733, 966
DrdI	GACNNNNNNGTC	2 cut(s)	134, 401
DseDI	GACNNNNNNGTC	2 cut(s)	134, 401
Eam1104I	CTCTTC	4 cut(s)	552, 561, 588, 994
EarI	CTCTTC	4 cut(s)	552, 561, 588, 994
Ecl136II	GAGCTC	2 cut(s)	106, 794
Eco130I	CCWWGG	1 cut(s)	408
Eco147I	AGGCCT	1 cut(s)	1166
Eco24I	GRGCYC	3 cut(s)	108, 796, 879
Eco32I	GATATC	1 cut(s)	646
Eco47I	GGWCC	2 cut(s)	563, 959
Eco53kI	GAGCTC	2 cut(s)	106, 794
Eco57I	CTGAAG	4 cut(s)	78, 542, 975, 1103
EcoICRI	GAGCTC	2 cut(s)	106, 794
EcoNI	CCTNNNNNAGG	1 cut(s)	1005
EcoO109I	RGGNCCY	2 cut(s)	563, 995
EcoRII	CCWGG	1 cut(s)	340
EcoRV	GATATC	1 cut(s)	646
EcoT14I	CCWWGG	1 cut(s)	408
EcoT22I	ATGCAT	1 cut(s)	730
EcoT38I	GRGCYC	3 cut(s)	108, 796, 879
ErhI	CCWWGG	1 cut(s)	408
Esp3I	CGTCTC	2 cut(s)	507, 523
FaeI	CATG	6 cut(s)	137, 181, 490, 525, 536, 885
FaqI	GGGAC	1 cut(s)	364
FatI	CATG	6 cut(s)	133, 177, 486, 521, 532, 881
FauNDI	CATATG	1 cut(s)	258
Fnu4HI	GCNGC	2 cut(s)	23, 573
FokI	GGATG	2 cut(s)	183, 462
FriOI	GRGCYC	3 cut(s)	108, 796, 879
Fsp4HI	GCNGC	2 cut(s)	23, 573
FspBI	CTAG	3 cut(s)	551, 672, 1005
GluI	GCNGC	2 cut(s)	23, 573
GsaI	CCCAGC	1 cut(s)	876
HaeIII	GGCC	5 cut(s)	267, 338, 503, 996, 1166
Hin1I	GRCGYC	2 cut(s)	46, 516
Hin1II	CATG	6 cut(s)	137, 181, 490, 525, 536, 885
HinfI	GANTC	8 cut(s)	197, 209, 271, 287, 395, 937, 1021, 1068
HphI	GGTGA	8 cut(s)	31, 71, 397, 451, 701, 784, 946, 1049
Hpy166II	GTNNAC	2 cut(s)	220, 684
Hpy188I	TCNGA	6 cut(s)	97, 626, 635, 763, 809, 1026
Hpy188III	TCNNGA	4 cut(s)	533, 716, 797, 1116
Hpy8I	GTNNAC	2 cut(s)	220, 684
Hpy99I	CGWCG	2 cut(s)	48, 84
HpyAV	CCTTC	8 cut(s)	61, 277, 370, 484, 763, 897, 905, 972
HpyCH4III	ACNGT	7 cut(s)	118, 217, 224, 236, 361, 1016, 1180
HpyCH4IV	ACGT	3 cut(s)	46, 516, 1107
HpyCH4V	TGCA	1 cut(s)	728
HpyF10VI	GCNNNNNNNGC	4 cut(s)	98, 326, 335, 500
HpyF3I	CTNAG	6 cut(s)	54, 102, 269, 709, 762, 930
HpySE526I	ACGT	3 cut(s)	46, 516, 1107
Hsp92I	GRCGYC	2 cut(s)	46, 516
Hsp92II	CATG	6 cut(s)	137, 181, 490, 525, 536, 885
Kzo9I	GATC	3 cut(s)	542, 733, 966
LguI	GCTCTTC	1 cut(s)	561
LmnI	GCTCC	4 cut(s)	481, 791, 847, 926
Lsp1109I	GCAGC	2 cut(s)	34, 559
MaeI	CTAG	3 cut(s)	551, 672, 1005
MaeII	ACGT	3 cut(s)	46, 516, 1107
MaeIII	GTNAC	5 cut(s)	403, 412, 1010, 1054, 1180
MalI	GATC	3 cut(s)	544, 735, 968
MboI	GATC	3 cut(s)	542, 733, 966
MboII	GAAGA	9 cut(s)	163, 539, 548, 575, 614, 704, 912, 981, 1096
MfeI	CAATTG	1 cut(s)	387
MflI	RGATCY	2 cut(s)	542, 733
MhlI	GDGCHC	5 cut(s)	108, 486, 658, 796, 879
MluCI	AATT	2 cut(s)	387, 636
MlyI	GAGTC	5 cut(s)	191, 203, 280, 281, 389
Mph1103I	ATGCAT	1 cut(s)	730
MroXI	GAANNNNTTC	1 cut(s)	494
MseI	TTAA	1 cut(s)	1143
MslI	CAYNNNNRTG	3 cut(s)	261, 531, 1187
MspR9I	CCNGG	1 cut(s)	342
MunI	CAATTG	1 cut(s)	387
Mva1269I	GAATGC	3 cut(s)	448, 494, 730
MvaI	CCWGG	1 cut(s)	342
MwoI	GCNNNNNNNGC	4 cut(s)	98, 326, 335, 500
NdeI	CATATG	1 cut(s)	258
NdeII	GATC	3 cut(s)	542, 733, 966
NlaIII	CATG	6 cut(s)	137, 181, 490, 525, 536, 885
NlaIV	GGNNCC	3 cut(s)	159, 339, 564
NmuCI	GTSAC	5 cut(s)	403, 412, 1010, 1054, 1180
NsiI	ATGCAT	1 cut(s)	730
NspI	RCATGY	1 cut(s)	181
PaeI	GCATGC	1 cut(s)	181
PagI	TCATGA	1 cut(s)	532
PceI	AGGCCT	1 cut(s)	1166
PciSI	GCTCTTC	1 cut(s)	561
PctI	GAATGC	3 cut(s)	448, 494, 730
PdmI	GAANNNNTTC	1 cut(s)	494
PfeI	GAWTC	3 cut(s)	937, 1021, 1068
PkrI	GCNGC	2 cut(s)	24, 574
PleI	GAGTC	5 cut(s)	191, 203, 279, 281, 389
PpsI	GAGTC	5 cut(s)	191, 203, 279, 281, 389
Ppu21I	YACGTR	1 cut(s)	1108
PpuMI	RGGWCCY	1 cut(s)	563
Psp124BI	GAGCTC	2 cut(s)	108, 796
Psp5II	RGGWCCY	1 cut(s)	563
Psp6I	CCWGG	1 cut(s)	340
PspFI	CCCAGC	1 cut(s)	872
PspGI	CCWGG	1 cut(s)	340
PspN4I	GGNNCC	3 cut(s)	159, 339, 564
PspPI	GGNCC	4 cut(s)	337, 563, 959, 995
PspPPI	RGGWCCY	1 cut(s)	563
PstNI	CAGNNNCTG	2 cut(s)	101, 716
PsuI	RGATCY	2 cut(s)	542, 733
RsaI	GTAC	3 cut(s)	683, 1106, 1200
RsaNI	GTAC	3 cut(s)	682, 1105, 1199
RseI	CAYNNNNRTG	3 cut(s)	261, 531, 1187
SacI	GAGCTC	2 cut(s)	108, 796
SapI	GCTCTTC	1 cut(s)	561
SaqAI	TTAA	1 cut(s)	1143
SatI	GCNGC	2 cut(s)	23, 573
Sau3AI	GATC	3 cut(s)	542, 733, 966
Sau96I	GGNCC	4 cut(s)	337, 563, 959, 995
SchI	GAGTC	5 cut(s)	191, 203, 280, 281, 389
ScrFI	CCNGG	1 cut(s)	342
SduI	GDGCHC	5 cut(s)	108, 486, 658, 796, 879
SfcI	CTRYAG	2 cut(s)	223, 888
SinI	GGWCC	2 cut(s)	563, 959
SmiMI	CAYNNNNRTG	3 cut(s)	261, 531, 1187
SmlI	CTYRAG	2 cut(s)	275, 302
SmoI	CTYRAG	2 cut(s)	275, 302
SphI	GCATGC	1 cut(s)	181
Sse9I	AATT	2 cut(s)	387, 636
SseBI	AGGCCT	1 cut(s)	1166
SspI	AATATT	1 cut(s)	1093
SspMI	CTAG	3 cut(s)	551, 672, 1005
SstI	GAGCTC	2 cut(s)	108, 796
StuI	AGGCCT	1 cut(s)	1166
StyD4I	CCNGG	1 cut(s)	340
StyI	CCWWGG	1 cut(s)	408
TaaI	ACNGT	7 cut(s)	118, 217, 224, 236, 361, 1016, 1180
TaiI	ACGT	3 cut(s)	49, 519, 1110
TaqI	TCGA	2 cut(s)	200, 965
TasI	AATT	2 cut(s)	387, 636
TfiI	GAWTC	3 cut(s)	937, 1021, 1068
Tru1I	TTAA	1 cut(s)	1143
Tru9I	TTAA	1 cut(s)	1143
TscAI	CASTG	6 cut(s)	220, 421, 665, 691, 870, 1021
TseFI	GTSAC	5 cut(s)	403, 412, 1010, 1054, 1180
TseI	GCWGC	2 cut(s)	22, 572
Tsp45I	GTSAC	5 cut(s)	403, 412, 1010, 1054, 1180
TspDTI	ATGAA	3 cut(s)	503, 521, 973
TspGWI	ACGGA	1 cut(s)	285
TspRI	CASTG	6 cut(s)	220, 421, 665, 691, 870, 1021
VpaK11BI	GGWCC	2 cut(s)	563, 959
XagI	CCTNNNNNAGG	1 cut(s)	1005
XapI	RAATTY	1 cut(s)	636
XceI	RCATGY	1 cut(s)	181
XcmI	CCANNNNNNNNNTGG	2 cut(s)	140, 748
XmnI	GAANNNNTTC	1 cut(s)	494
XspI	CTAG	3 cut(s)	551, 672, 1005
ZraI	GACGTC	2 cut(s)	47, 517
Zsp2I	ATGCAT	1 cut(s)	730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.