Rorug04G0398100

The glycine cleavage system catalyzes the degradation of glycine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007339
Physical Location & Seq
Forward (+)
56423951 .. 56425393
1443 bp
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UTR
Exon/CDS
Intron
Rorug04G0398100.1

Sequence Viewer

Length: 576 bp
ATGAACTTGCTTTGCTTTTTTTTTTTCCTGCAGTGTTTCAACAATACCCAATTTGCTACTTTAAATCTTTTTCATAGTATGGCTCTAGTGAATGTTGCTGCTCTCCAGTTTACAGCTTCTGCCCTTTTTGGGACTCGTTCTGCGACTTCAAACTTTCAGGTCTTGCAGACTAAGAGAAATTTCTCCATGAAGCCGGTAGTAATGAGAGCGAGGCAGCATGCCAGAACCGAAAGTGCGAAAACTCTAAACAGAAGAATGCAGAAGAAGTACAATGGCACGCCTAAGAAGCCGAGGCTTTCAGTATTCTGTTCAGACAAACAATTGTATGCTATGCTGGTAGATGACCAAAACAAGAAGTGTTTGTTTTATGGAAGCACTCTGCAGAAATCTATTCGCCAGGATCCCCACTGTAGTACCCCTGAAGCTGCAAAACGTGTTGGCGAGGAGTTAATCAAGGCCTGCAATGATCTCAACATAGGCGAAATATCGTCTTATGATCGCAATGGGTTTGCTACTGGGGAAAGGATTCATGCATTTGAGATTGCAATTTCAGAATACGGGTTCTTGTCAAGATAA

Protein Analysis

191

Amino Acids

21.75

Weight (kDa)

9.69

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 75 - 187 3.6e-16 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 395, 408
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 1 cut(s) 441
AfaI GTAC 2 cut(s) 269, 415
AfiI CCNNNNNNNGG 1 cut(s) 129
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 2 cut(s) 40, 150
AjnI CCWGG 1 cut(s) 396
AluBI AGCT 2 cut(s) 116, 425
AluI AGCT 2 cut(s) 116, 425
AlwI GGATC 2 cut(s) 395, 408
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 98, 214, 425
ApoI RAATTY 1 cut(s) 178
Asp700I GAANNNNTTC 1 cut(s) 525
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BamHI GGATCC 1 cut(s) 400
BbvI GCAGC 3 cut(s) 85, 226, 412
BciT130I CCWGG 1 cut(s) 398
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 3 cut(s) 29, 380, 409
BisI GCNGC 3 cut(s) 99, 215, 426
BlsI GCNGC 3 cut(s) 100, 216, 427
Bme1390I CCNGG 1 cut(s) 398
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 1 cut(s) 398
BmrI ACTGGG 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 525
BpmI CTGGAG 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 290
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 106, 520
Bse3DI GCAATG 2 cut(s) 469, 508
BseBI CCWGG 1 cut(s) 398
BseDI CCNNGG 1 cut(s) 290
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMI GCAATG 2 cut(s) 469, 508
BseNI ACTGG 2 cut(s) 106, 520
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 3 cut(s) 85, 226, 412
BshFI GGCC 1 cut(s) 458
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 261
BsnI GGCC 1 cut(s) 458
Bsp143I GATC 3 cut(s) 400, 466, 496
BspANI GGCC 1 cut(s) 458
BspLI GGNNCC 1 cut(s) 402
BspMAI CTGCAG 2 cut(s) 33, 384
BspPI GGATC 2 cut(s) 395, 408
BsrDI GCAATG 2 cut(s) 469, 508
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 2 cut(s) 106, 520
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 3 cut(s) 400, 466, 496
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 219, 278, 460
BstDEI CTNAG 2 cut(s) 171, 282
BstKTI GATC 3 cut(s) 403, 469, 499
BstMBI GATC 3 cut(s) 400, 466, 496
BstMWI GCNNNNNNNGC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 398
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 1 cut(s) 396
BstSFI CTRYAG 3 cut(s) 29, 380, 409
BstV1I GCAGC 3 cut(s) 85, 226, 412
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BsuRI GGCC 1 cut(s) 458
BtsI GCAGTG 1 cut(s) 38
BtsIMutI CAGTG 2 cut(s) 38, 406
Cac8I GCNNGC 3 cut(s) 219, 278, 460
CaiI CAGNNNCTG 1 cut(s) 119
Cfr10I RCCGGY 1 cut(s) 193
Csp6I GTAC 2 cut(s) 268, 414
CviAII CATG 3 cut(s) 187, 218, 530
CviJI RGCY 7 cut(s) 83, 116, 193, 289, 295, 425, 458
CviKI_1 RGCY 7 cut(s) 83, 116, 193, 289, 295, 425, 458
CviQI GTAC 2 cut(s) 268, 414
DdeI CTNAG 2 cut(s) 171, 282
DpnI GATC 3 cut(s) 402, 468, 498
DpnII GATC 3 cut(s) 400, 466, 496
DraI TTTAAA 1 cut(s) 63
Eco147I AGGCCT 1 cut(s) 458
Eco57I CTGAAG 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 396
EcoT22I ATGCAT 1 cut(s) 535
FaeI CATG 3 cut(s) 190, 221, 533
FaqI GGGAC 1 cut(s) 145
FatI CATG 3 cut(s) 186, 217, 529
Fnu4HI GCNGC 3 cut(s) 99, 215, 426
Fsp4HI GCNGC 3 cut(s) 99, 215, 426
FspBI CTAG 1 cut(s) 86
GluI GCNGC 3 cut(s) 99, 215, 426
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 1 cut(s) 458
HapII CCGG 1 cut(s) 194
Hin1II CATG 3 cut(s) 190, 221, 533
HinfI GANTC 2 cut(s) 133, 526
HpaII CCGG 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 2 cut(s) 313, 553
Hpy188III TCNNGA 1 cut(s) 570
Hpy8I GTNNAC 1 cut(s) 111
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 8 cut(s) 31, 166, 259, 382, 428, 462, 533, 545
HpyF10VI GCNNNNNNNGC 1 cut(s) 286
HpyF3I CTNAG 2 cut(s) 171, 282
HpySE526I ACGT 1 cut(s) 433
Hsp92II CATG 3 cut(s) 190, 221, 533
Kzo9I GATC 3 cut(s) 400, 466, 496
Lsp1109I GCAGC 3 cut(s) 85, 226, 412
MaeI CTAG 1 cut(s) 86
MaeII ACGT 1 cut(s) 433
MalI GATC 3 cut(s) 402, 468, 498
MboI GATC 3 cut(s) 400, 466, 496
MboII GAAGA 2 cut(s) 264, 274
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 400
MluCI AATT 4 cut(s) 50, 178, 320, 546
MlyI GAGTC 1 cut(s) 127
MnlI CCTC 3 cut(s) 204, 285, 436
Mph1103I ATGCAT 1 cut(s) 535
MroXI GAANNNNTTC 1 cut(s) 525
MseI TTAA 2 cut(s) 62, 449
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 398
MunI CAATTG 1 cut(s) 320
Mva1269I GAATGC 1 cut(s) 261
MvaI CCWGG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 286
NdeII GATC 3 cut(s) 400, 466, 496
NlaIII CATG 3 cut(s) 190, 221, 533
NlaIV GGNNCC 1 cut(s) 402
NmeAIII GCCGAG 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 535
NspI RCATGY 1 cut(s) 221
PaeI GCATGC 1 cut(s) 221
PceI AGGCCT 1 cut(s) 458
PctI GAATGC 1 cut(s) 261
PdmI GAANNNNTTC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 3 cut(s) 100, 216, 427
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 402
PstI CTGCAG 2 cut(s) 33, 384
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 2 cut(s) 269, 415
RsaNI GTAC 2 cut(s) 268, 414
SaqAI TTAA 2 cut(s) 62, 449
SatI GCNGC 3 cut(s) 99, 215, 426
Sau3AI GATC 3 cut(s) 400, 466, 496
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 398
SetI ASST 4 cut(s) 118, 162, 427, 436
SfcI CTRYAG 3 cut(s) 29, 380, 409
SphI GCATGC 1 cut(s) 221
Sse9I AATT 4 cut(s) 50, 178, 320, 546
SseBI AGGCCT 1 cut(s) 458
SspMI CTAG 1 cut(s) 86
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 410
TaiI ACGT 1 cut(s) 436
TasI AATT 4 cut(s) 50, 178, 320, 546
TatI WGTACW 1 cut(s) 267
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 2 cut(s) 62, 449
Tru9I TTAA 2 cut(s) 62, 449
TscAI CASTG 2 cut(s) 38, 413
TseI GCWGC 3 cut(s) 98, 214, 425
TspDTI ATGAA 4 cut(s) 17, 62, 203, 518
TspRI CASTG 2 cut(s) 38, 413
XapI RAATTY 1 cut(s) 178
XceI RCATGY 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 525
XspI CTAG 1 cut(s) 86
Zsp2I ATGCAT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.