MD10G1323700.v1.1

The glycine cleavage system catalyzes the degradation of glycine

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
Physical Location & Seq
Forward (+)
40464820 .. 40467953
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1323700.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGTTCTGTAATTGCATCTCATTGGATATTTCTGCTAAGATGAGAGGAGCAAGTTTGTGGCAGCTTGGCCAGTCAATCACCCGGCGTATTGCTCAGGGTGATAAGAAGGCTGTAGCTCGTCGATACTTTGCCTCAGAAGCTGAGCTAAAAAAGACAGTCCTCTATGACTTCCATGTTGCAAATGGCGGTAAGATGGTGCCTTTCGCTGGATGGGGAATGCCTATTCAGTACAAGGACTCAATCATCGATTCCACCATAAACTGCAGGCAGAATGGTAGTCTGTTTGATGTCTCCCATATGTGTGGGCTGAGCCTTAAGGGGAAGGATTCCATATCTTTCCTTGAAAAGCTTGTCATTGCTGATGTTGCTTCGCTTGCCCCTGGAACGGGGACACTCACTGTCTTTACAAATGAGAAGGGAGGGGCAATTGATGACTCCGTGATTACCAAGGTTACTGACGACCACATATACCTGGTGGTGAATGCTGGATGTAGGGATAAAGATCTTGCTCACATTGAAGAGCATATGAAGGCATTCAAGGCTAAAGGGGGAGATGTCTCGTGGCACATCCATGATGAGAGATCTCTTCTAGCTCTCCAGGGTCCTCTTGCTGCCCCAACCCTTCAACACTTGACAAAAGAGGATTTGAGCAAGTTATATTTTGGAGAGTTCCGAATTTTGGATATCAATGGTGCACACTGCTTTCTTACAAGGACTGGGTACACCGGTGAAGATGGATTTGAAATCTCGGTTCCTGATGAGAAAGCAGTAGATCTTGCCAAAGCAATCTTGGAGAAATCCGAGGGGAAGGTGAGGCTGACAGGTCTGGGTGCTCGTGACAGTCTCCGACTGGAAGCTGGGTTGTGTTTATATGGCAATGACATGGAGCAACACACAACACCTGTGGAGGCTGGACTCACATGGGCCATAGGGAAGAGAAGAAGGGCAGAGGGCGGCTTTCTTGGCGCTGAGGTAATACTCAAGCAACTCGAGGAAGGTCCTAAAATCCGGCGTGCTGGTTTCATCTCATCAGGTCCACCTGCTAGAAGCCACAGCGAGATTCAGAACGAGAAAGGTGAAACCATTGGGGAAGTCACAAGTGGAGGATTTAGCCCCTGCCTGAAGAAAAACATAGCGATGGGTTACGTGAAGTCTGGATCACACAAGGCCGGAACCAAAGTTAAGATCATTGTCCGAGGAAAGGCTTATGATGGAGCTGTCACGAAAATGCCTTTCGTACCTACAAAATACTACAAGCCAACATAA

Protein Analysis

422

Amino Acids

45.8

Weight (kDa)

8.81

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 54 - 312 1.6e-72 GCVT N-terminal domain
GCV_T_C PF08669 341 - 413 7.9e-18 Glycine cleavage T-protein C-terminal barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1048
Acc36I ACCTGC 1 cut(s) 1048
AccB1I GGYRCC 1 cut(s) 196
AciI CCGC 2 cut(s) 186, 954
AclWI GGATC 1 cut(s) 1165
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 1142
AfaI GTAC 3 cut(s) 230, 722, 1239
AfiI CCNNNNNNNGG 5 cut(s) 206, 385, 386, 679, 1201
AflII CTTAAG 1 cut(s) 314
AgeI ACCGGT 1 cut(s) 725
AgsI TTSAA 5 cut(s) 344, 518, 538, 626, 743
AjnI CCWGG 3 cut(s) 379, 471, 597
AluBI AGCT 8 cut(s) 64, 116, 140, 145, 349, 593, 857, 1217
AluI AGCT 8 cut(s) 64, 116, 140, 145, 349, 593, 857, 1217
Alw21I GWGCWC 2 cut(s) 697, 835
Alw26I GTCTC 3 cut(s) 295, 562, 848
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 1 cut(s) 1165
AlwNI CAGNNNCTG 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 989
AoxI GGCC 3 cut(s) 67, 924, 1167
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 2 cut(s) 61, 611
ApoI RAATTY 1 cut(s) 675
AsiGI ACCGGT 1 cut(s) 725
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 968
AspS9I GGNCC 4 cut(s) 602, 924, 998, 1034
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 6 cut(s) 70, 110, 490, 740, 823, 1088
AvaI CYCGRG 1 cut(s) 989
AvaII GGWCC 3 cut(s) 602, 998, 1034
BaeGI GKGCMC 1 cut(s) 697
BaeI ACNNNNGTAYC 2 cut(s) 712, 745
BalI TGGCCA 1 cut(s) 69
BanI GGYRCC 1 cut(s) 196
BauI CACGAG 2 cut(s) 559, 834
Bbv12I GWGCWC 2 cut(s) 697, 835
BbvCI CCTCAGC 1 cut(s) 969
BbvI GCAGC 2 cut(s) 73, 598
BccI CCATC 5 cut(s) 187, 204, 728, 1132, 1205
BciT130I CCWGG 3 cut(s) 381, 473, 599
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 295, 562, 848
BfaI CTAG 2 cut(s) 590, 1044
BfmI CTRYAG 2 cut(s) 111, 262
BfoI RGCGCY 1 cut(s) 969
BfrI CTTAAG 1 cut(s) 314
BfuAI ACCTGC 1 cut(s) 1048
BglII AGATCT 3 cut(s) 502, 581, 772
BisI GCNGC 3 cut(s) 62, 612, 955
BlpI GCTNAGC 2 cut(s) 141, 308
BlsI GCNGC 3 cut(s) 63, 613, 956
Bme1390I CCNGG 4 cut(s) 82, 381, 473, 599
Bme18I GGWCC 3 cut(s) 602, 998, 1034
BmeT110I CYCGRG 1 cut(s) 989
BmgT120I GGNCC 4 cut(s) 602, 924, 998, 1034
BmiI GGNNCC 4 cut(s) 198, 603, 753, 1174
BmrFI CCNGG 4 cut(s) 82, 381, 473, 599
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 1 cut(s) 24
BmuI ACTGGG 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 581
Bpu10I CCTNAGC 2 cut(s) 93, 969
Bpu1102I GCTNAGC 2 cut(s) 141, 308
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 1147
BsaBI GATNNNNATC 1 cut(s) 501
BsaJI CCNNGG 5 cut(s) 379, 447, 598, 801, 1195
BsaWI WCCGGW 1 cut(s) 725
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 385, 386, 679, 1201
Bse118I RCCGGY 1 cut(s) 725
Bse1I ACTGG 3 cut(s) 70, 721, 855
Bse3DI GCAATG 2 cut(s) 354, 883
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 3 cut(s) 381, 473, 599
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 5 cut(s) 379, 447, 598, 801, 1195
BseGI GGATG 3 cut(s) 215, 494, 567
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 5 cut(s) 206, 385, 386, 679, 1201
BseMI GCAATG 2 cut(s) 354, 883
BseMII CTCAG 5 cut(s) 107, 132, 147, 299, 960
BseNI ACTGG 3 cut(s) 70, 721, 855
BseRI GAGGAG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 2 cut(s) 73, 598
BseYI CCCAGC 1 cut(s) 857
BshFI GGCC 3 cut(s) 69, 926, 1169
BshNI GGYRCC 1 cut(s) 196
BshTI ACCGGT 1 cut(s) 725
BshVI ATCGAT 1 cut(s) 246
BsiHKAI GWGCWC 2 cut(s) 697, 835
BsiHKCI CYCGRG 1 cut(s) 989
BsiSI CCGG 4 cut(s) 82, 726, 1009, 1170
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 5 cut(s) 206, 385, 386, 679, 1201
BsmAI GTCTC 3 cut(s) 295, 562, 848
BsmFI GGGAC 1 cut(s) 403
BsmI GAATGC 3 cut(s) 222, 487, 533
BsnI GGCC 3 cut(s) 69, 926, 1169
BsoBI CYCGRG 1 cut(s) 989
Bsp1286I GDGCHC 2 cut(s) 697, 835
Bsp143I GATC 5 cut(s) 502, 581, 772, 1157, 1185
Bsp1720I GCTNAGC 2 cut(s) 141, 308
BspACI CCGC 2 cut(s) 186, 954
BspANI GGCC 3 cut(s) 69, 926, 1169
BspCNI CTCAG 5 cut(s) 106, 133, 146, 300, 961
BspDI ATCGAT 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 198, 603, 753, 1174
BspMAI CTGCAG 1 cut(s) 266
BspMI ACCTGC 1 cut(s) 1048
BspPI GGATC 1 cut(s) 1165
BspQI GCTCTTC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 196
BspTI CTTAAG 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 354, 883
BsrFI RCCGGY 1 cut(s) 725
BsrI ACTGG 3 cut(s) 70, 721, 855
BssAI RCCGGY 1 cut(s) 725
BssECI CCNNGG 5 cut(s) 379, 447, 598, 801, 1195
BssMI GATC 5 cut(s) 502, 581, 772, 1157, 1185
BssSI CACGAG 2 cut(s) 559, 834
BssT1I CCWWGG 1 cut(s) 447
Bst2BI CACGAG 2 cut(s) 559, 834
Bst2UI CCWGG 3 cut(s) 381, 473, 599
Bst4CI ACNGT 3 cut(s) 157, 400, 842
Bst6I CTCTTC 3 cut(s) 513, 591, 929
BstAFI CTTAAG 1 cut(s) 314
BstBAI YACGTR 1 cut(s) 1147
BstC8I GCNNGC 3 cut(s) 266, 375, 1014
BstDEI CTNAG 6 cut(s) 36, 93, 133, 141, 308, 969
BstF5I GGATG 3 cut(s) 215, 494, 567
BstH2I RGCGCY 1 cut(s) 969
BstHHI GCGC 1 cut(s) 968
BstKTI GATC 5 cut(s) 505, 584, 775, 1160, 1188
BstMAI GTCTC 3 cut(s) 295, 562, 848
BstMBI GATC 5 cut(s) 502, 581, 772, 1157, 1185
BstMWI GCNNNNNNNGC 5 cut(s) 137, 365, 374, 539, 963
BstNI CCWGG 3 cut(s) 381, 473, 599
BstSCI CCNGG 4 cut(s) 80, 379, 471, 597
BstSFI CTRYAG 2 cut(s) 111, 262
BstSLI GKGCMC 1 cut(s) 697
BstV1I GCAGC 2 cut(s) 73, 598
BstX2I RGATCY 3 cut(s) 502, 581, 772
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 3 cut(s) 502, 581, 772
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 3 cut(s) 69, 926, 1169
BsuTUI ATCGAT 1 cut(s) 246
BtgZI GCGATG 1 cut(s) 1151
BtsCI GGATG 3 cut(s) 215, 494, 567
BtsI GCAGTG 1 cut(s) 697
BtsIMutI CAGTG 2 cut(s) 396, 697
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 3 cut(s) 266, 375, 1014
CaiI CAGNNNCTG 1 cut(s) 140
CfoI GCGC 1 cut(s) 968
Cfr10I RCCGGY 1 cut(s) 725
Cfr13I GGNCC 4 cut(s) 602, 924, 998, 1034
ClaI ATCGAT 1 cut(s) 246
CsiI ACCWGGT 1 cut(s) 471
Csp6I GTAC 3 cut(s) 229, 721, 1238
CspAI ACCGGT 1 cut(s) 725
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CviAII CATG 4 cut(s) 173, 572, 883, 921
CviQI GTAC 3 cut(s) 229, 721, 1238
DdeI CTNAG 6 cut(s) 36, 93, 133, 141, 308, 969
DpnI GATC 5 cut(s) 504, 583, 774, 1159, 1187
DpnII GATC 5 cut(s) 502, 581, 772, 1157, 1185
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 3 cut(s) 513, 591, 929
EarI CTCTTC 3 cut(s) 513, 591, 929
Eco130I CCWWGG 1 cut(s) 447
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 3 cut(s) 602, 998, 1034
Eco57I CTGAAG 1 cut(s) 1142
Eco88I CYCGRG 1 cut(s) 989
EcoO109I RGGNCCY 2 cut(s) 602, 998
EcoRII CCWGG 3 cut(s) 379, 471, 597
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 447
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 4 cut(s) 176, 575, 886, 924
FaqI GGGAC 1 cut(s) 403
FatI CATG 4 cut(s) 172, 571, 882, 920
FauNDI CATATG 2 cut(s) 297, 525
Fnu4HI GCNGC 3 cut(s) 62, 612, 955
FokI GGATG 3 cut(s) 222, 501, 554
Fsp4HI GCNGC 3 cut(s) 62, 612, 955
FspBI CTAG 2 cut(s) 590, 1044
GlaI GCGC 1 cut(s) 967
GluI GCNGC 3 cut(s) 62, 612, 955
GsaI CCCAGC 1 cut(s) 861
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 969
HaeIII GGCC 3 cut(s) 69, 926, 1169
HapII CCGG 4 cut(s) 82, 726, 1009, 1170
HhaI GCGC 1 cut(s) 968
Hin1II CATG 4 cut(s) 176, 575, 886, 924
Hin6I GCGC 1 cut(s) 966
HinP1I GCGC 1 cut(s) 966
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 6 cut(s) 236, 248, 326, 434, 915, 1060
HpaII CCGG 4 cut(s) 82, 726, 1009, 1170
HphI GGTGA 6 cut(s) 70, 110, 490, 740, 823, 1088
Hpy166II GTNNAC 3 cut(s) 695, 723, 1037
Hpy188I TCNGA 6 cut(s) 136, 674, 802, 848, 1065, 1196
Hpy188III TCNNGA 4 cut(s) 755, 836, 1155, 1222
Hpy8I GTNNAC 3 cut(s) 695, 723, 1037
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 8 cut(s) 100, 316, 409, 523, 632, 802, 936, 989
HpyCH4III ACNGT 3 cut(s) 157, 400, 842
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 4 cut(s) 15, 179, 264, 695
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 365, 374, 539, 963
HpyF3I CTNAG 6 cut(s) 36, 93, 133, 141, 308, 969
HpySE526I ACGT 1 cut(s) 1146
Hsp92II CATG 4 cut(s) 176, 575, 886, 924
HspAI GCGC 1 cut(s) 966
Kzo9I GATC 5 cut(s) 502, 581, 772, 1157, 1185
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 3 cut(s) 47, 886, 1214
Lsp1109I GCAGC 2 cut(s) 73, 598
LweI GCATC 1 cut(s) 24
MabI ACCWGGT 1 cut(s) 471
MaeI CTAG 2 cut(s) 590, 1044
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 5 cut(s) 451, 836, 1093, 1142, 1219
MalI GATC 5 cut(s) 504, 583, 774, 1159, 1187
MboI GATC 5 cut(s) 502, 581, 772, 1157, 1185
MboII GAAGA 6 cut(s) 530, 578, 743, 946, 951, 1135
MfeI CAATTG 1 cut(s) 426
MflI RGATCY 3 cut(s) 502, 581, 772
MhlI GDGCHC 2 cut(s) 697, 835
MlsI TGGCCA 1 cut(s) 69
MluCI AATT 3 cut(s) 10, 426, 675
MluNI TGGCCA 1 cut(s) 69
MlyI GAGTC 3 cut(s) 230, 428, 909
MmeI TCCRAC 1 cut(s) 871
Mox20I TGGCCA 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 533
MscI TGGCCA 1 cut(s) 69
MseI TTAA 2 cut(s) 315, 1182
MslI CAYNNNNRTG 4 cut(s) 300, 570, 1136, 1226
Msp20I TGGCCA 1 cut(s) 69
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 4 cut(s) 82, 726, 1009, 1170
MspR9I CCNGG 4 cut(s) 82, 381, 473, 599
MunI CAATTG 1 cut(s) 426
Mva1269I GAATGC 3 cut(s) 222, 487, 533
MvaI CCWGG 3 cut(s) 381, 473, 599
MwoI GCNNNNNNNGC 5 cut(s) 137, 365, 374, 539, 963
NciI CCSGG 1 cut(s) 82
NdeI CATATG 2 cut(s) 297, 525
NdeII GATC 5 cut(s) 502, 581, 772, 1157, 1185
NlaIII CATG 4 cut(s) 176, 575, 886, 924
NlaIV GGNNCC 4 cut(s) 198, 603, 753, 1174
NmuCI GTSAC 3 cut(s) 836, 1093, 1219
PaeR7I CTCGAG 1 cut(s) 989
PaqCI CACCTGC 1 cut(s) 1048
PciSI GCTCTTC 1 cut(s) 513
PctI GAATGC 3 cut(s) 222, 487, 533
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 248, 326, 1060
PinAI ACCGGT 1 cut(s) 725
PkrI GCNGC 3 cut(s) 63, 613, 956
PleI GAGTC 3 cut(s) 230, 428, 909
PpsI GAGTC 3 cut(s) 230, 428, 909
Ppu21I YACGTR 1 cut(s) 1147
PpuMI RGGWCCY 2 cut(s) 602, 998
Psp5II RGGWCCY 2 cut(s) 602, 998
Psp6I CCWGG 3 cut(s) 379, 471, 597
PspFI CCCAGC 1 cut(s) 857
PspGI CCWGG 3 cut(s) 379, 471, 597
PspN4I GGNNCC 4 cut(s) 198, 603, 753, 1174
PspPI GGNCC 4 cut(s) 602, 924, 998, 1034
PspPPI RGGWCCY 2 cut(s) 602, 998
PspXI VCTCGAGB 1 cut(s) 989
PstI CTGCAG 1 cut(s) 266
PstNI CAGNNNCTG 1 cut(s) 140
PsuI RGATCY 3 cut(s) 502, 581, 772
RsaI GTAC 3 cut(s) 230, 722, 1239
RsaNI GTAC 3 cut(s) 229, 721, 1238
RseI CAYNNNNRTG 4 cut(s) 300, 570, 1136, 1226
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 2 cut(s) 315, 1182
SatI GCNGC 3 cut(s) 62, 612, 955
Sau3AI GATC 5 cut(s) 502, 581, 772, 1157, 1185
Sau96I GGNCC 4 cut(s) 602, 924, 998, 1034
SchI GAGTC 3 cut(s) 230, 428, 909
ScrFI CCNGG 4 cut(s) 82, 381, 473, 599
SduI GDGCHC 2 cut(s) 697, 835
SexAI ACCWGGT 1 cut(s) 471
SfaNI GCATC 1 cut(s) 24
SfcI CTRYAG 2 cut(s) 111, 262
Sfr274I CTCGAG 1 cut(s) 989
SgrAI CRCCGGYG 1 cut(s) 725
SinI GGWCC 3 cut(s) 602, 998, 1034
SlaI CTCGAG 1 cut(s) 989
SmiMI CAYNNNNRTG 4 cut(s) 300, 570, 1136, 1226
SmlI CTYRAG 3 cut(s) 314, 980, 989
SmoI CTYRAG 3 cut(s) 314, 980, 989
Sse9I AATT 3 cut(s) 10, 426, 675
SsiI CCGC 2 cut(s) 186, 954
SspMI CTAG 2 cut(s) 590, 1044
StyD4I CCNGG 4 cut(s) 80, 379, 471, 597
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 157, 400, 842
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 3 cut(s) 121, 246, 990
TasI AATT 3 cut(s) 10, 426, 675
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 957
TfiI GAWTC 3 cut(s) 248, 326, 1060
Tru1I TTAA 2 cut(s) 315, 1182
Tru9I TTAA 2 cut(s) 315, 1182
TscAI CASTG 2 cut(s) 403, 704
TseFI GTSAC 3 cut(s) 836, 1093, 1219
TseI GCWGC 2 cut(s) 61, 611
Tsp45I GTSAC 3 cut(s) 836, 1093, 1219
TspDTI ATGAA 2 cut(s) 542, 1012
TspGWI ACGGA 1 cut(s) 427
TspRI CASTG 2 cut(s) 403, 704
Vha464I CTTAAG 1 cut(s) 314
VneI GTGCAC 1 cut(s) 693
VpaK11BI GGWCC 3 cut(s) 602, 998, 1034
XapI RAATTY 1 cut(s) 675
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 787
XhoI CTCGAG 1 cut(s) 989
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 2 cut(s) 590, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.