FvH4_3g02460

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004422
Physical Location & Seq
Reverse (-)
1225360 .. 1232457
7098 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g02460.t5

Sequence Viewer

Length: 3981 bp
ATGTCCTGGGGTTTGGGATGGAAGCGCCCCTCCGAGATCTTCCATCTCACGCTCACCTACGGCACGGAGGACCCGCCGCCGCCGCCGGAGAATCCCGCCTCCGCCACGCCTCCGTCGTCGTCCTCCGCCTCCGCGTCTTCGTCGTCGATCTCGTCGATAGTGTCGCAGCAGGATCAGGAGCCGGGGTTCCGGATCGATCTGGATTGGTCCGCCGGCGACGACGAGGACCAGGTGGCGCTGCGGCTCCAGTCGCAGCTCATGGTGGCGCTGCCGATGCCGCAGGACACGGTGGTGGTGGAGCTGAGGCCGGAGGAGGCGAATGTGAGTGTGGATATGAGGGTTGTGAGGCGGAGAGAGCCGTTGAGGGCGGTTAGTATGGCCAAGACGGGCGGGTCGGGTCAGCAGAATGACGGTACTGGAGTCTTGACCCGGTTGTTGAGGTCAAATTTCTCTTCGTCAATGCCAGCTGTCGCTGAAGGTACGGCGGCTTGCGGCGTGCATTGGCAGTGCGTCACCGTGGTCCGTTTATGTGGCTGTGGTTTGTCGGTATTCCCGGTAGAGTTAACAAGACTGCCTCTTCTTGAGAAGCTATACCTTGATAACAATAAACTGGCACTTTTGCCTGCTGAACTCGGTGAATTGAGAAGCTTAAAGGTGCTCAGGGTTGACTACAACATGCTGGCTTCAGTACCTGTGGAACTGAGACAGTGTGTTGGATTAGTGGAACTTTCTTTGGAACACAACAAGCTTATTCGGCCTCTGCTTGATTTCAGGGCTATGGCTGAACTCCGCGTACTTAGGCTATTTGGAAACCCTCTTGAATTTCTTCCTGAAATCTTGCCTCTGCACAAACTTCGTCATTTATCTCTTGCAAATATCAGGATTGTGGCAGACGACAGCTTGAGATCAGTGAATGTGCAGATAGAGATGGAAAACAGTTCGTATTTTGGTGCATCTAGGCATAAGCTAAGTGCCTTTTTCGCTCTTATATTTCGTTTTTCTTCTTGTCATCACCCGTTACTAGCATCTGCATTAGCAAAGATAATGCAAGACGAAGGGAACCGTGCCGTGGTTGGTAAAGATGAGAATGCAGTGCGACAGCTTATTAGTATGATCAGCAGTGACAACCGTCATGTGGTTCAACAAGCATGTTCTGCACTTTCATCTCTTGCCGCAGATGTTTCAGTTGCAATGCAGTTGATCAAAGCTGATGTTATGCAACCCATTGAGACGGTACTGAGATCTGTTCTCCAAGAGGAAGTAATTTCTGTATTGCAAGTTGTGGTGAAGCTGGCCTTTGCATCTGATGCTGTAGCTCAGAAAATGCTGACCAAGGATGTATTGAAGTCTTTAAAACACTTATGTGCCCACAAAACCCCAGAGGTGCAAAGGTTAGCTCTGTTAGCAGTTGGGAATTTGGCATTCTGTTTGGAGAATCGTCGTCTTCTGGTTACTTCTGAAAGTTTATGTGAACTTCTCATGCGCTTGACAGTTGCACCTGAACCGCGTGTTAACAAAGCTGCAGCTCGTGCTTTGGCAATTCTTGGGGAAAATGGAAATCTACGACGTGCCATAAGGGGGAGACAGGTGCCAAAACAAGGATTGCGCATACTCTCAATGGATGGTGGTGGTATGAAAGGCCTGGCAACTGTGCAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACCAATACATGAGTTGTTTGACCTCATATGCGGCACATCAACGGGTGGCATGCTTGGTGTGGCTCTTGGCATTAAGCTAATGTCATTAGATCAGTGTGAAGAAATCTACAAAAACCTTGGAAAACTTGTCTTTGCTGAACCTGCTCCAAAGGACAATGAAGCTGCAAGTTGGAGAGAGAAGTTGGATCAGCTCTATAAAAGTTCATCACAGAGTTTTCGAGTTGTTGTACATGGATCTAAACATAGCGCAGATCAGTTCGAGAGACTATTGAAGGAAATGTGTGCTGATGAGGAAGGAGATCTGTTGATAGAGTCAGCAGTGAAAAACATTCCCAAAGTTTTTGTAGTGTCAACTTTGGTGAGCATGACGCCAGCGCAGCCGTTCTTATTCCGGAATTATCAGTACCCTGTGGGAACGCCAGAAGTAGCTTTTGTAGGTTCAGAGAGTTCAGGAATCACTGTGCAAGAACCAACTAGTTTGGGTACTGAACTTGGCTATAGACGCAGTGCTTTTATGGGAAGTTGCAAGCATCAAATATGGCAAGCGATAAGAGCCTCATCTGCTGCACCATACTATCTTGATGATTTCTCAGATGATATTCATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACTATCTTTGCCATCAGAGAAGCACAACTTTTATGGCCTGACACGAAAATTGATTGCTTAGTTTCTATTGGCTGTGGTTCTGTTCCGACAAAGGTGCGAAAAGGTGGTTGGCGATATCTTGATACTGGGCAAGTATTGATAGAGAGTTCTTGCTCTGTGGAACGCGTGGAGGAAGCCTTAAGTACATTGCTACCGATGCTCCCTGGGATACAATACTTTCGGTTTAATCCAGTTGACGAACGTTGTGATATGGAACTGGATGAGACTGATCCAGCTGTCTGGCTAAAATTGGAAGCCTCGGTGGAAGAATATGTTCAGAAAAGTTCTCTTGCACTTAAGGATGCCTGTGAGAGACTACTGCTGCCATTCCAAAATGACGAAAAGTGGTCCGAGAGTTTGAGATCCCAACATTTCCCCAAGGCAAATGAGGAAGTGAAAAACCCATCTCTAGGTTGGAGGCGTAATGTGCTACTTGTTGAAGCTTCGCATAGCCCCAATTCTGGTCGAGCTTTGAATCATGCTCATGAACTTGAATCATTTTGTGCTCGTAATGGAATACGGGTATCTTTAATGCAAGGAATCTCAGGGTTTGTGAAGACAACACCAGCAGCAACATTCCCAACACCATTTCAGTCACCATTGTTTCCTGCAAGTGTCCCATCAAGCCCACTTTTCTACAGTCCTGACTTTGGCCCGCAGAGGGTTGGCCGAATTGATATGGTCCCACCTCTGAGCTTAGATGGTCAACCTGGAAAGGGAGCCGCCTCACCACCAAAGTCTCCTTCAGGGCCTAGACAGCTTTCTGTACCTGTTCAGTCGTTGCATGATAAGTTACAAAATTCGCCACAAGTGGGCATTGTGCATTTGGCCCTTCAAAATGACTCAATTGGCTCAATTTTAAGTTGGCAAAATGATGTATTTGTGGTTGCTGAACCTGGAGAGCTTGCAGATAACTTTCTGCAGAGTGTTAAGTTGAGCTTGTTATCAAATATGCGAAACCACCGCAAGAAGGCTGGATCATCTCTTTCCAATATTTCAACTGTTTCTGATTTAGTTGCATATAAACCATGCTTCCAACTTGGATGTATCGTTCACCGTTATATGGGACGCCAAACTCAAGTAATGGAAGATGGCCAAGAAATTGGAGCGTATTTGTTTCGTAGAACTGTCCCCTCTATTCACCTAACACCTGATGACGTTCGATGGATGGTTGGAGCTTGGAGAGACAGGATCATTATTTGTACAGGGACAAATGGGCCTACGCCAACTTTGATCAAAGCATTTTTAGACTCTGGTGCCAAAGCGGTTATATCTTCTTCAATACAGCCCCAAAAAACACAGTTAACATTCGGGTCTACTGATTTCAGTGTTCTTGAAAAGTTTGAGATTGGTGAGGAGGAGGAGGCTGAGGAAGACGAAGTTGAAGATGAGGCGACTGAACCTGAAAGTCCGGTGAGTGATTGGGAAGATAGTGAAAATGAAAACCGCTCTATCGGCTTTGGGGATGATGATGAGGAAGAAGTGTCCCAATTCGTCTGCCATTTGTATGACTCACTGTTTCGAGAGGGTGTGAGTGTGGATGTTGCTTTACGACATGCTCTTGCCTCACATCGGAAGTTGAGGTATACGTGCCATCTTCCCAGTATACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1327

Amino Acids

146.03

Weight (kDa)

5.71

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 193 - 250 5.6e-06 Leucine rich repeat
Patatin PF01734 538 - 780 5.5e-20 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
Acc16I TGCGCA 1 cut(s) 1607
Acc36I ACCTGC 1 cut(s) 1837
AccB1I GGYRCC 2 cut(s) 1588, 3623
AccBSI CCGCTC 1 cut(s) 3816
AccI GTMKAC 3 cut(s) 3683, 3953, 3973
AccII CGCG 4 cut(s) 134, 792, 1507, 2493
AccIII TCCGGA 2 cut(s) 189, 2079
AclI AACGTT 1 cut(s) 2569
AclWI GGATC 8 cut(s) 180, 200, 1881, 1930, 2591, 2724, 3352, 3566
AcoI YGGCCR 3 cut(s) 378, 3034, 3460
AcsI RAATTY 5 cut(s) 445, 821, 1414, 1656, 3166
AcuI CTGAAG 3 cut(s) 495, 669, 3096
AcyI GRCGYC 2 cut(s) 2057, 3436
AflII CTTAAG 2 cut(s) 2506, 2663
AflIII ACRYGT 1 cut(s) 2491
AhlI ACTAGT 1 cut(s) 2162
AjiI CACGTC 1 cut(s) 1568
AjnI CCWGG 6 cut(s) 5, 228, 1641, 2530, 3076, 3262
AjuI GAANNNNNNNTTGG 4 cut(s) 2419, 2451, 3094, 3126
AleI CACNNNNGTG 2 cut(s) 290, 1362
AloI GAACNNNNNNTCC 4 cut(s) 170, 202, 3402, 3434
Alw21I GWGCWC 2 cut(s) 660, 2875
Alw26I GTCTC 8 cut(s) 697, 1223, 1576, 1945, 2585, 2674, 3111, 3546
AlwI GGATC 8 cut(s) 180, 200, 1881, 1930, 2591, 2724, 3352, 3566
AlwNI CAGNNNCTG 1 cut(s) 692
Aor13HI TCCGGA 2 cut(s) 189, 2079
ApoI RAATTY 5 cut(s) 445, 821, 1414, 1656, 3166
Asp700I GAANNNNTTC 2 cut(s) 825, 2640
AspLEI GCGC 7 cut(s) 27, 238, 268, 1485, 1608, 1937, 2065
AsuC2I CCSGG 3 cut(s) 183, 430, 554
AvaII GGWCC 6 cut(s) 70, 207, 226, 520, 2715, 3049
BaeGI GKGCMC 1 cut(s) 1369
BaeI ACNNNNGTAYC 2 cut(s) 405, 438
BalI TGGCCA 2 cut(s) 380, 3462
BanI GGYRCC 2 cut(s) 1588, 3623
BauI CACGAG 1 cut(s) 1527
BbsI GAAGAC 4 cut(s) 129, 1436, 2930, 3747
Bbv12I GWGCWC 2 cut(s) 660, 2875
BbvCI CCTCAGC 2 cut(s) 302, 3735
BceAI ACGGC 5 cut(s) 76, 343, 498, 1052, 2053
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 2404, 2438
BciT130I CCWGG 6 cut(s) 7, 230, 1643, 2532, 3078, 3264
BciVI GTATCC 1 cut(s) 2529
BclI TGATCA 3 cut(s) 1113, 1200, 3600
BcnI CCSGG 3 cut(s) 183, 430, 554
BcoDI GTCTC 8 cut(s) 697, 1223, 1576, 1945, 2585, 2674, 3111, 3546
BcuI ACTAGT 1 cut(s) 2162
BfaI CTAG 5 cut(s) 957, 1022, 2163, 2777, 3120
BfmI CTRYAG 5 cut(s) 1311, 1521, 2185, 3004, 3287
BfoI RGCGCY 3 cut(s) 28, 239, 269
BfrI CTTAAG 2 cut(s) 2506, 2663
BfuAI ACCTGC 1 cut(s) 1837
BfuI GTATCC 1 cut(s) 2529
BglII AGATCT 3 cut(s) 36, 1241, 1987
Bme1390I CCNGG 9 cut(s) 7, 183, 230, 430, 554, 1643, 2532, 3078, 3264
Bme18I GGWCC 6 cut(s) 70, 207, 226, 520, 2715, 3049
BmgBI CACGTC 1 cut(s) 1568
BmiI GGNNCC 9 cut(s) 72, 180, 188, 245, 1061, 1590, 3051, 3088, 3625
BmrFI CCNGG 9 cut(s) 7, 183, 230, 430, 554, 1643, 2532, 3078, 3264
BmrI ACTGGG 2 cut(s) 2463, 3963
BmsI GCATC 8 cut(s) 264, 962, 1034, 1297, 1310, 2227, 2514, 2659
BmuI ACTGGG 2 cut(s) 2463, 3963
BoxI GACNNNNGTC 1 cut(s) 1128
BpiI GAAGAC 4 cut(s) 129, 1436, 2930, 3747
BpmI CTGGAG 3 cut(s) 230, 438, 3285
Bpu10I CCTNAGC 3 cut(s) 302, 659, 3735
BpuEI CTTGAG 3 cut(s) 602, 922, 3429
BpuMI CCSGG 3 cut(s) 183, 430, 554
Bsa29I ATCGAT 1 cut(s) 195
BsaAI YACGTR 1 cut(s) 3957
BsaBI GATNNNNATC 1 cut(s) 2289
BsaHI GRCGYC 2 cut(s) 2057, 3436
BsaWI WCCGGW 3 cut(s) 189, 2079, 3778
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 2511, 2541
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 8 cut(s) 247, 421, 615, 1687, 2458, 2558, 2589, 3969
Bse3DI GCAATG 2 cut(s) 1197, 2513
Bse8I GATNNNNATC 1 cut(s) 2289
BseAI TCCGGA 2 cut(s) 189, 2079
BseBI CCWGG 6 cut(s) 7, 230, 1643, 2532, 3078, 3264
BseCI ATCGAT 1 cut(s) 195
BseGI GGATG 9 cut(s) 23, 1342, 1627, 2593, 2674, 3416, 3540, 3838, 3913
BseJI GATNNNNATC 1 cut(s) 2289
BseMI GCAATG 2 cut(s) 1197, 2513
BseMII CTCAG 9 cut(s) 293, 673, 692, 1229, 1331, 2292, 2925, 3050, 3726
BseNI ACTGG 8 cut(s) 247, 421, 615, 1687, 2458, 2558, 2589, 3969
BseRI GAGGAG 4 cut(s) 326, 3737, 3740, 3743
BseSI GKGCMC 1 cut(s) 1369
BsgI GTGCAG 4 cut(s) 830, 938, 1140, 2238
Bsh1236I CGCG 4 cut(s) 134, 792, 1507, 2493
BshNI GGYRCC 2 cut(s) 1588, 3623
BshVI ATCGAT 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 660, 2875
BsiSI CCGG 9 cut(s) 86, 182, 190, 213, 308, 430, 554, 2080, 3779
BslFI GGGAC 6 cut(s) 2969, 3035, 3447, 3482, 3589, 3838
BsmAI GTCTC 8 cut(s) 697, 1223, 1576, 1945, 2585, 2674, 3111, 3546
BsmBI CGTCTC 1 cut(s) 1223
BsmFI GGGAC 6 cut(s) 2969, 3035, 3447, 3482, 3589, 3838
BsmI GAATGC 2 cut(s) 1093, 1421
Bsp1286I GDGCHC 3 cut(s) 660, 1369, 2875
Bsp13I TCCGGA 2 cut(s) 189, 2079
Bsp1407I TGTACA 2 cut(s) 1915, 3569
BspCNI CTCAG 9 cut(s) 294, 672, 693, 1230, 1330, 2291, 2924, 3051, 3727
BspDI ATCGAT 1 cut(s) 195
BspEI TCCGGA 2 cut(s) 189, 2079
BspFNI CGCG 4 cut(s) 134, 792, 1507, 2493
BspHI TCATGA 1 cut(s) 2851
BspLI GGNNCC 9 cut(s) 72, 180, 188, 245, 1061, 1590, 3051, 3088, 3625
BspMAI CTGCAG 2 cut(s) 1525, 3291
BspMI ACCTGC 1 cut(s) 1837
BspPI GGATC 8 cut(s) 180, 200, 1881, 1930, 2591, 2724, 3352, 3566
BspT107I GGYRCC 2 cut(s) 1588, 3623
BspTI CTTAAG 2 cut(s) 2506, 2663
BsrBI CCGCTC 1 cut(s) 3816
BsrDI GCAATG 2 cut(s) 1197, 2513
BsrFI RCCGGY 1 cut(s) 212
BsrGI TGTACA 2 cut(s) 1915, 3569
BsrI ACTGG 8 cut(s) 247, 421, 615, 1687, 2458, 2558, 2589, 3969
BssAI RCCGGY 1 cut(s) 212
BssNAI GTATAC 2 cut(s) 3954, 3974
BssNI GRCGYC 2 cut(s) 2057, 3436
BssSI CACGAG 1 cut(s) 1527
BssT1I CCWWGG 3 cut(s) 1332, 1804, 2745
Bst1107I GTATAC 2 cut(s) 3954, 3974
Bst2BI CACGAG 1 cut(s) 1527
Bst2UI CCWGG 6 cut(s) 7, 230, 1643, 2532, 3078, 3264
Bst6I CTCTTC 2 cut(s) 457, 582
BstACI GRCGYC 2 cut(s) 2057, 3436
BstAFI CTTAAG 2 cut(s) 2506, 2663
BstAPI GCANNNNNTGC 3 cut(s) 1154, 1307, 1529
BstAUI TGTACA 2 cut(s) 1915, 3569
BstBAI YACGTR 1 cut(s) 3957
BstDSI CCRYGG 2 cut(s) 516, 1068
BstF5I GGATG 9 cut(s) 23, 1342, 1627, 2593, 2674, 3416, 3540, 3838, 3913
BstFNI CGCG 4 cut(s) 134, 792, 1507, 2493
BstH2I RGCGCY 3 cut(s) 28, 239, 269
BstHHI GCGC 7 cut(s) 27, 238, 268, 1485, 1608, 1937, 2065
BstMAI GTCTC 8 cut(s) 697, 1223, 1576, 1945, 2585, 2674, 3111, 3546
BstNI CCWGG 6 cut(s) 7, 230, 1643, 2532, 3078, 3264
BstNSI RCATGY 4 cut(s) 679, 1152, 1741, 3926
BstPAI GACNNNNGTC 1 cut(s) 1128
BstSCI CCNGG 9 cut(s) 5, 181, 228, 428, 552, 1641, 2530, 3076, 3262
BstSFI CTRYAG 5 cut(s) 1311, 1521, 2185, 3004, 3287
BstSLI GKGCMC 1 cut(s) 1369
BstUI CGCG 4 cut(s) 134, 792, 1507, 2493
BstV2I GAAGAC 4 cut(s) 129, 1436, 2930, 3747
BstX2I RGATCY 5 cut(s) 36, 1241, 1922, 1987, 2729
BstXI CCANNNNNNTGG 2 cut(s) 2607, 3470
BstYI RGATCY 5 cut(s) 36, 1241, 1922, 1987, 2729
BstZ17I GTATAC 2 cut(s) 3954, 3974
Bsu15I ATCGAT 1 cut(s) 195
BsuI GTATCC 1 cut(s) 2529
BsuTUI ATCGAT 1 cut(s) 195
BtgI CCRYGG 2 cut(s) 516, 1068
BtrI CACGTC 1 cut(s) 1568
BtsCI GGATG 9 cut(s) 23, 1342, 1627, 2593, 2674, 3416, 3540, 3838, 3913
BtsI GCAGTG 5 cut(s) 512, 1098, 1126, 2013, 2200
BveI ACCTGC 1 cut(s) 1837
CaiI CAGNNNCTG 1 cut(s) 692
CciI TCATGA 1 cut(s) 2851
CfoI GCGC 7 cut(s) 27, 238, 268, 1485, 1608, 1937, 2065
Cfr10I RCCGGY 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 195
CseI GACGC 5 cut(s) 123, 499, 2065, 2199, 3444
CsiI ACCWGGT 1 cut(s) 228
DraI TTTAAA 1 cut(s) 1353
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 3 cut(s) 378, 3034, 3460
Eam1104I CTCTTC 2 cut(s) 457, 582
EarI CTCTTC 2 cut(s) 457, 582
EciI GGCGGA 4 cut(s) 91, 115, 199, 364
Eco130I CCWWGG 3 cut(s) 1332, 1804, 2745
Eco147I AGGCCT 1 cut(s) 1641
Eco32I GATATC 1 cut(s) 2444
Eco47I GGWCC 6 cut(s) 70, 207, 226, 520, 2715, 3049
Eco57I CTGAAG 3 cut(s) 495, 669, 3096
EcoO109I RGGNCCY 2 cut(s) 70, 3116
EcoRII CCWGG 6 cut(s) 5, 228, 1641, 2530, 3076, 3262
EcoRV GATATC 1 cut(s) 2444
EcoT14I CCWWGG 3 cut(s) 1332, 1804, 2745
ErhI CCWWGG 3 cut(s) 1332, 1804, 2745
Esp3I CGTCTC 1 cut(s) 1223
FalI AAGNNNNNCTT 6 cut(s) 1280, 1312, 2340, 2372, 3290, 3322
FaqI GGGAC 6 cut(s) 2969, 3035, 3447, 3482, 3589, 3838
FauI CCCGC 4 cut(s) 81, 103, 383, 3030
FauNDI CATATG 1 cut(s) 1715
FbaI TGATCA 3 cut(s) 1113, 1200, 3600
FblI GTMKAC 3 cut(s) 3683, 3953, 3973
FokI GGATG 9 cut(s) 30, 1349, 1634, 2600, 2681, 3423, 3547, 3845, 3920
FspBI CTAG 5 cut(s) 957, 1022, 2163, 2777, 3120
FspI TGCGCA 1 cut(s) 1607
GlaI GCGC 7 cut(s) 26, 237, 267, 1484, 1607, 1936, 2064
GsuI CTGGAG 3 cut(s) 230, 438, 3285
HaeII RGCGCY 3 cut(s) 28, 239, 269
HapII CCGG 9 cut(s) 86, 182, 190, 213, 308, 430, 554, 2080, 3779
HgaI GACGC 5 cut(s) 123, 499, 2065, 2199, 3444
HhaI GCGC 7 cut(s) 27, 238, 268, 1485, 1608, 1937, 2065
Hin1I GRCGYC 2 cut(s) 2057, 3436
Hin6I GCGC 7 cut(s) 25, 236, 266, 1483, 1606, 1935, 2063
HinP1I GCGC 7 cut(s) 25, 236, 266, 1483, 1606, 1935, 2063
HincII GTYRAC 7 cut(s) 564, 667, 1513, 2040, 2563, 3074, 3672
HindII GTYRAC 7 cut(s) 564, 667, 1513, 2040, 2563, 3074, 3672
HindIII AAGCTT 3 cut(s) 646, 746, 2808
HpaI GTTAAC 3 cut(s) 564, 1513, 3672
HpaII CCGG 9 cut(s) 86, 182, 190, 213, 308, 430, 554, 2080, 3779
Hpy99I CGWCG 9 cut(s) 118, 121, 145, 148, 157, 221, 224, 1443, 1569
HpyAV CCTTC 8 cut(s) 470, 1049, 1669, 1954, 1976, 3120, 3209, 3331
HpyCH4IV ACGT 4 cut(s) 1567, 2569, 3525, 3956
HpySE526I ACGT 4 cut(s) 1567, 2569, 3525, 3956
Hsp92I GRCGYC 2 cut(s) 2057, 3436
HspAI GCGC 7 cut(s) 25, 236, 266, 1483, 1606, 1935, 2063
Kpn2I TCCGGA 2 cut(s) 189, 2079
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 3 cut(s) 1113, 1200, 3600
KspAI GTTAAC 3 cut(s) 564, 1513, 3672
LmnI GCTCC 8 cut(s) 178, 249, 298, 1837, 2532, 3086, 3473, 3542
LweI GCATC 8 cut(s) 264, 962, 1034, 1297, 1310, 2227, 2514, 2659
MabI ACCWGGT 1 cut(s) 228
MaeI CTAG 5 cut(s) 957, 1022, 2163, 2777, 3120
MaeII ACGT 4 cut(s) 1567, 2569, 3525, 3956
MaeIII GTNAC 6 cut(s) 511, 1017, 1121, 1450, 2961, 3159
MbiI CCGCTC 1 cut(s) 3816
MfeI CAATTG 2 cut(s) 1668, 3213
MflI RGATCY 5 cut(s) 36, 1241, 1922, 1987, 2729
MhlI GDGCHC 3 cut(s) 660, 1369, 2875
MlsI TGGCCA 2 cut(s) 380, 3462
MluI ACGCGT 1 cut(s) 2491
MluNI TGGCCA 2 cut(s) 380, 3462
MlyI GAGTC 5 cut(s) 429, 2009, 3203, 3611, 3872
MmeI TCCRAC 7 cut(s) 694, 1838, 1851, 2438, 2762, 3427, 3520
Mox20I TGGCCA 2 cut(s) 380, 3462
MreI CGCCGGCG 1 cut(s) 212
MroI TCCGGA 2 cut(s) 189, 2079
MroNI GCCGGC 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 825, 2640
MscI TGGCCA 2 cut(s) 380, 3462
MslI CAYNNNNRTG 4 cut(s) 290, 1362, 2850, 3873
Msp20I TGGCCA 2 cut(s) 380, 3462
MspA1I CMGCKG 2 cut(s) 467, 2603
MspCI CTTAAG 2 cut(s) 2506, 2663
MspI CCGG 9 cut(s) 86, 182, 190, 213, 308, 430, 554, 2080, 3779
MspR9I CCNGG 9 cut(s) 7, 183, 230, 430, 554, 1643, 2532, 3078, 3264
MunI CAATTG 2 cut(s) 1668, 3213
Mva1269I GAATGC 2 cut(s) 1093, 1421
MvaI CCWGG 6 cut(s) 7, 230, 1643, 2532, 3078, 3264
MvnI CGCG 4 cut(s) 134, 792, 1507, 2493
NaeI GCCGGC 1 cut(s) 214
NciI CCSGG 3 cut(s) 183, 430, 554
NdeI CATATG 1 cut(s) 1715
NgoMIV GCCGGC 1 cut(s) 212
NlaIV GGNNCC 9 cut(s) 72, 180, 188, 245, 1061, 1590, 3051, 3088, 3625
NmuCI GTSAC 3 cut(s) 511, 1121, 2961
NsbI TGCGCA 1 cut(s) 1607
NspI RCATGY 4 cut(s) 679, 1152, 1741, 3926
OliI CACNNNNGTG 2 cut(s) 290, 1362
PaeI GCATGC 1 cut(s) 1741
PagI TCATGA 1 cut(s) 2851
PasI CCCWGGG 1 cut(s) 2531
PceI AGGCCT 1 cut(s) 1641
PcsI WCGNNNNNNNCGW 3 cut(s) 113, 149, 152
PctI GAATGC 2 cut(s) 1093, 1421
PdiI GCCGGC 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 825, 2640
PfeI GAWTC 6 cut(s) 91, 1435, 2142, 2842, 2861, 2907
PleI GAGTC 5 cut(s) 428, 2008, 3203, 3611, 3872
PpsI GAGTC 5 cut(s) 428, 2008, 3203, 3611, 3872
Ppu21I YACGTR 1 cut(s) 3957
PpuMI RGGWCCY 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 1128
Psp1406I AACGTT 1 cut(s) 2569
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 6 cut(s) 5, 228, 1641, 2530, 3076, 3262
PspGI CCWGG 6 cut(s) 5, 228, 1641, 2530, 3076, 3262
PspN4I GGNNCC 9 cut(s) 72, 180, 188, 245, 1061, 1590, 3051, 3088, 3625
PspPPI RGGWCCY 1 cut(s) 70
PsrI GAACNNNNNNTAC 2 cut(s) 777, 809
PstI CTGCAG 2 cut(s) 1525, 3291
PstNI CAGNNNCTG 1 cut(s) 692
PsuI RGATCY 5 cut(s) 36, 1241, 1922, 1987, 2729
PvuII CAGCTG 2 cut(s) 467, 2603
RseI CAYNNNNRTG 4 cut(s) 290, 1362, 2850, 3873
SchI GAGTC 5 cut(s) 429, 2009, 3203, 3611, 3872
ScrFI CCNGG 9 cut(s) 7, 183, 230, 430, 554, 1643, 2532, 3078, 3264
SduI GDGCHC 3 cut(s) 660, 1369, 2875
SexAI ACCWGGT 1 cut(s) 228
SfaNI GCATC 8 cut(s) 264, 962, 1034, 1297, 1310, 2227, 2514, 2659
SfcI CTRYAG 5 cut(s) 1311, 1521, 2185, 3004, 3287
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 6 cut(s) 70, 207, 226, 520, 2715, 3049
SmiMI CAYNNNNRTG 4 cut(s) 290, 1362, 2850, 3873
SmlI CTYRAG 5 cut(s) 581, 901, 2506, 2663, 3444
SmoI CTYRAG 5 cut(s) 581, 901, 2506, 2663, 3444
SpeI ACTAGT 1 cut(s) 2162
SphI GCATGC 1 cut(s) 1741
SseBI AGGCCT 1 cut(s) 1641
SspI AATATT 1 cut(s) 3361
SspMI CTAG 5 cut(s) 957, 1022, 2163, 2777, 3120
StuI AGGCCT 1 cut(s) 1641
StyD4I CCNGG 9 cut(s) 5, 181, 228, 428, 552, 1641, 2530, 3076, 3262
StyI CCWWGG 3 cut(s) 1332, 1804, 2745
TaiI ACGT 4 cut(s) 1570, 2572, 3528, 3959
TaqI TCGA 8 cut(s) 146, 155, 195, 1906, 1947, 2833, 3529, 3889
TatI WGTACW 3 cut(s) 1915, 2510, 3569
TfiI GAWTC 6 cut(s) 91, 1435, 2142, 2842, 2861, 2907
TseFI GTSAC 3 cut(s) 511, 1121, 2961
Tsp45I GTSAC 3 cut(s) 511, 1121, 2961
TspDTI ATGAA 7 cut(s) 1152, 1649, 1860, 1881, 2279, 2868, 3822
TspGWI ACGGA 3 cut(s) 80, 102, 512
Vha464I CTTAAG 2 cut(s) 2506, 2663
VpaK11BI GGWCC 6 cut(s) 70, 207, 226, 520, 2715, 3049
XapI RAATTY 5 cut(s) 445, 821, 1414, 1656, 3166
XceI RCATGY 4 cut(s) 679, 1152, 1741, 3926
XcmI CCANNNNNNNNNTGG 1 cut(s) 2778
XmiI GTMKAC 3 cut(s) 3683, 3953, 3973
XmnI GAANNNNTTC 2 cut(s) 825, 2640
XspI CTAG 5 cut(s) 957, 1022, 2163, 2777, 3120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.