Rroxscaffold_1G00072250

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004422
Physical Location & Seq
Forward (+)
93391877 .. 93400696
8820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00072250.1

Sequence Viewer

Length: 4470 bp
ATGTCCTGGGGGCTGGGATGGAAGCGCCCCTCCGAGATCTTCCATCTCACGCTCTCCTACGGCACCGAGGACCCGCCGACGCCGGAGAATTCCAGCGCCGGCACGCGGTCCTCGGCGTCCTCGTTGGAGTCTTCCTCGTCGGTAGTGTCGCAGCAAGATCAAGAATTGGGGTTCCGGATCGATCTGGATTGGTCCGCCGGCGACGACGAGGACCAGGTGGCGCTGCGGCTCCAGTCGCAGCTGATGGTGGCGCTGCCGATGCCGCAGGACACGGTGGTCGTGGAGCTGAGGCCGGAGGAGGCGAATGTGAGTGTGGATATGAGGGTTGTGCGGCGGAGAGAGCCACTGAGGGCGGTGAGTATGGCCAAGACGGGCGGGTCGGGTCAGCAGAATGACGGCACCGGAGTGTTGACCCGGTTGTTGAGGTCCAATTTCTCTTCGTCGATGCCAGCCGTCGCCGAAGGCGTGGCGGCTTGCGGTGTGCATTGGCAGTGCGTCACGGTGGTCCGTCTCTGTGGTTGTGGTTTGTCGGTATTGCCGGTAGAGCTAACCAGACTGCCTCTTCTTGAGAAGTTACACCTTGATAACAATAAACTGGAACGTTTGCCTTCTGAACTTGGTGAATTGAGAAGCTTAAAGGTGCTCAGGGTTGACTACAACATGCTGGTTTCAGTACCGGCAGTGGAACTGAGACAGTGTGTTGGATTAGTGGAACTTTCTTTGGAACACAACAAGCTTGTTCGGCCTCTGCTTGATTTCAGGGCTATGGCTGAGCTCCGGGTACTTAGGCTATTTGGAAACCCTCTTGAATTTCTTCCTGAAATCTTGCCTCTGCACAAACTTCATCATTTATCTCTCGCAAATATCAGGATTGTGGCAGATGACAGCTTGAGATCAGTGAATGTGCAGATAGAGATGGAAAACAGTTCGTATTTTGGTGCATCTAGGCATAAGCTAAGCGCCTTTTTTGCCCTTATATTCCGTTTTTCTTCTTGTCATCACCCTTTACTAGCATCTGCACTAGCAAAGATAATGCAAGACGAAGGAAACCGTGCAGTTGTTGGTAAAGATGAGAATGCAGTGCGACAGCTTATTAGTATGATCAGTAGTGATGACCGTCATGTGGTTCAAGAAGCATGTTCTGCTCTTTCATCTCTTGCTGCAGACGTTTCAGTTGCAATGCAGTTGATGAAAGCCGACATTATGCAACCCATTGAGACGGTACTGAGATCTGTTCTCCAAGAGGAAGTGATTTCTGTATTACAAGTTGTGGTGAAGCTGGCTTTTGCATCTGATGCCGTAGCTCAGAAAATGCTGACCAAGGATGTGCAAAGGTTAGCTTTGGTAGCAGTTGGAAATTTGGCGTTCTGTTTGGAGAATCGTCGCCTTCTGGTTACTTCTGAAAGTTTGTGTGAACTTCTCATGCGCTTAACGGTTGTGCCTGAACCACGTGTGAATAAAGCTGCAGCTCGTGCTTTGGCAATTCTTGGGGAAAATGGAAATCTACGACGTGCCATAAGGGGGAGACAGGTGCCAAAGCAAGGATTACGCATACTCTCAATGGATGGTGGTGGTATGAAAGGTCTGGCAACTGTGCAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACCCATACATGAGTTGTTTGACCTCATATGCGGCACATCAACTGGTGGCATGCTTGCTGTTGCTCTTGGCATTAAGCTAATGTCATTAGATCAGTGTGAAGAAATATACAAAAACCTTGGTGAGAATGCTGTATTAACTCGAAGCTTTTCTCATGGATCAATTGTATGTATCAGTTCTGATATAAGTTTTGCAGGAAAACTTGTCTTTGCTGAACCTGCTCCAAAGGACAATGAAGCTGCAAGTTGGAGAGAGAAGTTGGATCAGCTTTATAAAAGTTCGTCGCAGAGTTTCAGAGTTGTTGTACATGGATCTAAACATAGTGCAGATCAGTTTGAGACATTGTTGAAGGAAATGTGTGCTGATGAGGATGGAGATCTGATGATAGAGTCGGCAGTAAAAAACATTCCCAAAGTTTTTGTAGTGTCAACTTTGGTGAGCATGACGCCAGCTCAGCCGTTCTTATTCCGGAATTATCAGTACCCTGCGGGAACGCCAGAAGTAGCTCTTGTAGTTTCGGAGAGTTCAGGAATCACTGTGCAAGAACCAACTACTTTGGGTACTGACCTTGGCTATAAACGCAGTGCTTTTATGGGAAGTTGTAAGCATCAAATATGGCAAGCTATAAGAGCATCATCTGCTGCGCCATACTATCTTGATGATTTCTCAGATGATATTCATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTGCCATCAGAGAAGCACAACTTTTATGGCCTGACACGAAAATTGATTGCTTAGTTTCTGTTGGCTGTGGTTCTGTTCCGACAAAGGTACGAAAAGGTGGTTGGCGTTATCTGGATACTGGGCAAGTATTGATAGAGAGTTCTTGCTCTGTGGAACGTGTGGAGGAAGCCTTGAGCACATTGCTACCCATGCTCCCTGGAATACAATACTTTCGGTTTAATCCAGTTGATGAACGTTGTGATATGGAACTGGATGAGACTGATCCAGCTGTCTGGCTGAAATTGGAAGCCTCGGTTGAGGAATATATTCAGAAAAATTCTCTTACTCTTAAGGATGCCTGTGAGAGACTACTGCTGCCATTCCAAAACGACGAAAAGTGGTCCGAGAATTTGAGATCCCAACATTTCCCCAAGGCAAATGAGGGTATTGATGCTAACATTTCTGTCATTATTCTGCCCTATGCATTTTTAGGTTTTTTGAAACTCAAGTATTATTGCTACTTTGCTGCAGTAGATGTGAAAAGCCCATCTCTAGGTTGGAGGCGTAATGTGCTACTTGTTGAAGCTTCTCATAGCCCCAATTCTGGTCGAGCTTCGAATCACGCTCATGAACTTGAATCATTTTGTGCTCGTAATGGAATACGGGTATCTTTAATGCAAGGAATATCAGGGTTTGTGAAAACCACGGCAGCAACAACATTCCCAACACCATTTCAGTCGCCTTTGTTTCCTGCAAGCGTCCCATCAAGCCCACTTTTCTACAGTCCTGACTTTGGCCCGCAGAGGGTTGGCCGAATCGATATGGTCCCACCTCTGAGCTTAGATGGCCAACCTGGAAAGGGAGCTGCCTCACCACCAAAGTCTCCTTCAGGACCTAGGCAGCTTTCTGTACCTGTTCAGTCATTGCATGAGAAGTTACAAAATTCGCCACAAGTGGGCATTGTGCATTTGGCCCTTCAAAATGACTCAATTGGCTCGATTTTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAACCTGGAGAACTTGCAGATAACTTTCTGCAGAGTGTTAAGTTGAGCTTGTTATCAGTTATGCGAAACCATCGCAAGAAGGCTGGATCACCTCTTTCCAATATTTCAACTGTTTCTGATTTGGTTGCTTGTAAACCAATCTTCCAACTTGGATGTATTGTTCACCGTTATATGGGACGCCAAACTCAAGTAATGGAAGATGGCCAAGAAATTGGGGCGTATTTGTTTCGTAGAACTGTCCCCTCTATTCACTTGACACCTGATGATGTTCGATGGATGGTTGGAGCTTGGAGAGACAGGATCATTATTTGTACAGGGACAAATGGGCTTACGCCAACTATGATCAAAGCATTTTTAGACTCTGGTGCCAAAGCGGTTATATCTTCGTCAGTACAGCCCCAAGAAACACAGTTAACATTCGGGTCTACTGATTTCAGTGTTCTTGAAAAGTTTGAGATTGGTGATGAGGAGGCTGAAGATGAAGAAGTTGAAGATGAGGCGGCTGAACCTGAAAGTCCGGTGAGTGATTGGGAAGACAGTGAAAATGGAGACCGCTCTATTGGCTTCGGGGATGATGATGAGGAAGAAGTGTCCCAATTCGTCTGTCATTTGTATGACTCACTGTTTCGAGAGGGTGCGAGTGTGGATGTTGCTTTACGACATGCTCTTGCCTCACATCGGAAGTTGAGGTATTTGTGTCATCTTCCCAACATGGAGTATAAAACCAGCACCACAGAAGCCAAGTTTCCTGAGACTCGAAAAATGGCTGCAACAGCTCCAGTTGCAATAGGCACGCGAGGCACTGTGGGATCTCTTGTCAGGAGGGAGATTGAGTACTTTGGTAAGCTTGAGCTAGACCGGCATGGAAGTTCAGTGAAGCCCCGGGGGAAAATGGTGGACATGAGTTCCAGGAATTGTGATTCTAGCAGGCCTAGTTTCTGGTTCTTGAGGATGACCTGGAAATGGAAGAGGCAAAGAGGGAGCAGGAGGTTCTTATCAAACATATGTTCTGCGGCGGAAGTTGTAGAAAACAATCGGCTAAGTGGCATTCCTGGGTTCAATTATAGGTCCCTCAAGGATGATATGAACAACTTGAGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1489

Amino Acids

164.4

Weight (kDa)

5.91

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 170 - 266 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 188 - 246 1.2e-07 Leucine rich repeat
Patatin PF01734 519 - 786 8.5e-20 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
AasI GACNNNNNNGTC 2 cut(s) 275, 376
Acc36I ACCTGC 1 cut(s) 1855
AccB1I GGYRCC 4 cut(s) 62, 398, 1531, 3731
AccBSI CCGCTC 1 cut(s) 3921
AccI GTMKAC 1 cut(s) 3791
AccII CGCG 2 cut(s) 106, 4162
AccIII TCCGGA 2 cut(s) 174, 2097
AclI AACGTT 2 cut(s) 601, 2587
AclWI GGATC 9 cut(s) 185, 1795, 1899, 1948, 2609, 2742, 3460, 3674, 4183
AcoI YGGCCR 4 cut(s) 363, 3142, 3178, 3568
AcsI RAATTY 7 cut(s) 88, 809, 1357, 1599, 2668, 2740, 3274
AcuI CTGAAG 2 cut(s) 3204, 3861
AcvI CACGTG 1 cut(s) 1451
AcyI GRCGYC 4 cut(s) 80, 116, 2075, 3544
AflII CTTAAG 1 cut(s) 2681
AflIII ACRYGT 2 cut(s) 1450, 2509
AjiI CACGTC 1 cut(s) 1511
AjnI CCWGG 8 cut(s) 5, 213, 2548, 3184, 3370, 4274, 4322, 4417
AjuI GAANNNNNNNTTGG 4 cut(s) 2437, 2469, 3202, 3234
AleI CACNNNNGTG 1 cut(s) 404
AloI GAACNNNNNNTCC 2 cut(s) 3510, 3542
Alw21I GWGCWC 4 cut(s) 645, 777, 2531, 2983
AlwI GGATC 9 cut(s) 185, 1795, 1899, 1948, 2609, 2742, 3460, 3674, 4183
Ama87I CYCGRG 1 cut(s) 4248
Aor13HI TCCGGA 2 cut(s) 174, 2097
ApoI RAATTY 7 cut(s) 88, 809, 1357, 1599, 2668, 2740, 3274
Asp700I GAANNNNTTC 3 cut(s) 813, 1777, 2658
AspA2I CCTAGG 1 cut(s) 3227
AspLEI GCGC 7 cut(s) 27, 98, 223, 253, 962, 1428, 2275
AsuC2I CCSGG 4 cut(s) 415, 779, 4249, 4250
AsuII TTCGAA 1 cut(s) 2948
AvaI CYCGRG 1 cut(s) 4248
AvrII CCTAGG 1 cut(s) 3227
BalI TGGCCA 3 cut(s) 365, 3180, 3570
BanI GGYRCC 4 cut(s) 62, 398, 1531, 3731
BanII GRGCYC 1 cut(s) 777
BauI CACGAG 1 cut(s) 1470
BbrPI CACGTG 1 cut(s) 1451
BbsI GAAGAC 2 cut(s) 123, 3906
Bbv12I GWGCWC 4 cut(s) 645, 777, 2531, 2983
BbvCI CCTCAGC 1 cut(s) 287
BceAI ACGGC 6 cut(s) 76, 412, 437, 1283, 2071, 3054
BciT130I CCWGG 8 cut(s) 7, 215, 2550, 3186, 3372, 4276, 4324, 4419
BciVI GTATCC 1 cut(s) 2461
BclI TGATCA 2 cut(s) 1101, 3708
BcnI CCSGG 4 cut(s) 415, 779, 4249, 4250
BfaI CTAG 8 cut(s) 945, 1010, 1022, 2885, 3228, 4220, 4290, 4299
BfmI CTRYAG 5 cut(s) 1161, 1464, 2859, 3112, 3395
BfoI RGCGCY 5 cut(s) 28, 99, 224, 254, 963
BfrI CTTAAG 1 cut(s) 2681
BfuAI ACCTGC 1 cut(s) 1855
BfuI GTATCC 1 cut(s) 2461
BglII AGATCT 3 cut(s) 36, 1229, 2005
BlnI CCTAGG 1 cut(s) 3227
BlpI GCTNAGC 3 cut(s) 771, 956, 2082
BmcAI AGTACT 1 cut(s) 4202
BmeT110I CYCGRG 1 cut(s) 4248
BmgBI CACGTC 1 cut(s) 1511
BmiI GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1533, 3159, 3733, 4436
BmrI ACTGGG 1 cut(s) 2481
BmuI ACTGGG 1 cut(s) 2481
BpiI GAAGAC 2 cut(s) 123, 3906
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 3 cut(s) 215, 3393, 4128
Bpu10I CCTNAGC 2 cut(s) 287, 644
Bpu1102I GCTNAGC 3 cut(s) 771, 956, 2082
Bpu14I TTCGAA 1 cut(s) 2948
BpuEI CTTGAG 8 cut(s) 587, 910, 2545, 2822, 3537, 4235, 4333, 4424
BpuMI CCSGG 4 cut(s) 415, 779, 4249, 4250
Bsa29I ATCGAT 2 cut(s) 180, 3150
BsaAI YACGTR 1 cut(s) 1451
BsaBI GATNNNNATC 2 cut(s) 2004, 2307
BsaHI GRCGYC 4 cut(s) 80, 116, 2075, 3544
BsaI GGTCTC 1 cut(s) 3909
BsaWI WCCGGW 4 cut(s) 174, 401, 2097, 3883
BsaXI ACNNNNNCTCC 4 cut(s) 2529, 2559, 4184, 4214
Bse118I RCCGGY 5 cut(s) 98, 197, 538, 676, 4224
Bse1I ACTGG 8 cut(s) 232, 600, 1630, 1678, 2476, 2576, 2607, 4145
Bse3DI GCAATG 3 cut(s) 1185, 2531, 3254
Bse8I GATNNNNATC 2 cut(s) 2004, 2307
BseAI TCCGGA 2 cut(s) 174, 2097
BseBI CCWGG 8 cut(s) 7, 215, 2550, 3186, 3372, 4276, 4324, 4419
BseCI ATCGAT 2 cut(s) 180, 3150
BseJI GATNNNNATC 2 cut(s) 2004, 2307
BseMI GCAATG 3 cut(s) 1185, 2531, 3254
BseNI ACTGG 8 cut(s) 232, 600, 1630, 1678, 2476, 2576, 2607, 4145
BseRI GAGGAG 2 cut(s) 311, 3848
BseYI CCCAGC 1 cut(s) 13
BsgI GTGCAG 5 cut(s) 818, 926, 1002, 1074, 1974
Bsh1236I CGCG 2 cut(s) 106, 4162
BshNI GGYRCC 4 cut(s) 62, 398, 1531, 3731
BshVI ATCGAT 2 cut(s) 180, 3150
BsiHKAI GWGCWC 4 cut(s) 645, 777, 2531, 2983
BsiHKCI CYCGRG 1 cut(s) 4248
BslFI GGGAC 7 cut(s) 3077, 3143, 3555, 3590, 3697, 3943, 4420
BsmBI CGTCTC 2 cut(s) 515, 1211
BsmFI GGGAC 7 cut(s) 3077, 3143, 3555, 3590, 3697, 3943, 4420
BsmI GAATGC 3 cut(s) 1081, 1762, 4413
Bso31I GGTCTC 1 cut(s) 3909
BsoBI CYCGRG 1 cut(s) 4248
Bsp119I TTCGAA 1 cut(s) 2948
Bsp1286I GDGCHC 4 cut(s) 645, 777, 2531, 2983
Bsp13I TCCGGA 2 cut(s) 174, 2097
Bsp1407I TGTACA 2 cut(s) 1933, 3677
Bsp1720I GCTNAGC 3 cut(s) 771, 956, 2082
BspDI ATCGAT 2 cut(s) 180, 3150
BspEI TCCGGA 2 cut(s) 174, 2097
BspFNI CGCG 2 cut(s) 106, 4162
BspHI TCATGA 1 cut(s) 2959
BspLI GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1533, 3159, 3733, 4436
BspMAI CTGCAG 4 cut(s) 1165, 1468, 2863, 3399
BspMI ACCTGC 1 cut(s) 1855
BspPI GGATC 9 cut(s) 185, 1795, 1899, 1948, 2609, 2742, 3460, 3674, 4183
BspT104I TTCGAA 1 cut(s) 2948
BspT107I GGYRCC 4 cut(s) 62, 398, 1531, 3731
BspTI CTTAAG 1 cut(s) 2681
BspTNI GGTCTC 1 cut(s) 3909
BsrBI CCGCTC 1 cut(s) 3921
BsrDI GCAATG 3 cut(s) 1185, 2531, 3254
BsrFI RCCGGY 5 cut(s) 98, 197, 538, 676, 4224
BsrGI TGTACA 2 cut(s) 1933, 3677
BsrI ACTGG 8 cut(s) 232, 600, 1630, 1678, 2476, 2576, 2607, 4145
BssAI RCCGGY 5 cut(s) 98, 197, 538, 676, 4224
BssNI GRCGYC 4 cut(s) 80, 116, 2075, 3544
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 5 cut(s) 1320, 1747, 2197, 2763, 3227
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 8 cut(s) 7, 215, 2550, 3186, 3372, 4276, 4324, 4419
Bst6I CTCTTC 3 cut(s) 442, 567, 4328
BstACI GRCGYC 4 cut(s) 80, 116, 2075, 3544
BstAFI CTTAAG 1 cut(s) 2681
BstAPI GCANNNNNTGC 4 cut(s) 1142, 1295, 1472, 2267
BstAUI TGTACA 2 cut(s) 1933, 3677
BstBAI YACGTR 1 cut(s) 1451
BstBI TTCGAA 1 cut(s) 2948
BstDSI CCRYGG 1 cut(s) 3036
BstFNI CGCG 2 cut(s) 106, 4162
BstH2I RGCGCY 5 cut(s) 28, 99, 224, 254, 963
BstHHI GCGC 7 cut(s) 27, 98, 223, 253, 962, 1428, 2275
BstNI CCWGG 8 cut(s) 7, 215, 2550, 3186, 3372, 4276, 4324, 4419
BstNSI RCATGY 4 cut(s) 664, 1140, 1684, 4031
BstSFI CTRYAG 5 cut(s) 1161, 1464, 2859, 3112, 3395
BstUI CGCG 2 cut(s) 106, 4162
BstV2I GAAGAC 2 cut(s) 123, 3906
BstX2I RGATCY 6 cut(s) 36, 1229, 1940, 2005, 2747, 4175
BstXI CCANNNNNNTGG 2 cut(s) 2625, 3578
BstYI RGATCY 6 cut(s) 36, 1229, 1940, 2005, 2747, 4175
Bsu15I ATCGAT 2 cut(s) 180, 3150
BsuI GTATCC 1 cut(s) 2461
BsuTUI ATCGAT 2 cut(s) 180, 3150
BtgI CCRYGG 1 cut(s) 3036
BtgZI GCGATG 1 cut(s) 3422
BtrI CACGTC 1 cut(s) 1511
BtsI GCAGTG 4 cut(s) 497, 687, 1086, 2218
BveI ACCTGC 1 cut(s) 1855
CciI TCATGA 1 cut(s) 2959
CfoI GCGC 7 cut(s) 27, 98, 223, 253, 962, 1428, 2275
Cfr10I RCCGGY 5 cut(s) 98, 197, 538, 676, 4224
Cfr9I CCCGGG 1 cut(s) 4248
ClaI ATCGAT 2 cut(s) 180, 3150
CseI GACGC 6 cut(s) 88, 105, 484, 2083, 3079, 3552
CsiI ACCWGGT 1 cut(s) 213
DrdI GACNNNNNNGTC 2 cut(s) 275, 376
DseDI GACNNNNNNGTC 2 cut(s) 275, 376
EaeI YGGCCR 4 cut(s) 363, 3142, 3178, 3568
Eam1104I CTCTTC 3 cut(s) 442, 567, 4328
EarI CTCTTC 3 cut(s) 442, 567, 4328
EciI GGCGGA 3 cut(s) 184, 349, 4397
Ecl136II GAGCTC 1 cut(s) 775
Eco130I CCWWGG 5 cut(s) 1320, 1747, 2197, 2763, 3227
Eco147I AGGCCT 1 cut(s) 4297
Eco24I GRGCYC 1 cut(s) 777
Eco31I GGTCTC 1 cut(s) 3909
Eco53kI GAGCTC 1 cut(s) 775
Eco57I CTGAAG 2 cut(s) 3204, 3861
Eco72I CACGTG 1 cut(s) 1451
Eco88I CYCGRG 1 cut(s) 4248
EcoICRI GAGCTC 1 cut(s) 775
EcoO109I RGGNCCY 3 cut(s) 70, 3224, 4434
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 8 cut(s) 5, 213, 2548, 3184, 3370, 4274, 4322, 4417
EcoT14I CCWWGG 5 cut(s) 1320, 1747, 2197, 2763, 3227
EcoT22I ATGCAT 1 cut(s) 2818
EcoT38I GRGCYC 1 cut(s) 777
ErhI CCWWGG 5 cut(s) 1320, 1747, 2197, 2763, 3227
Esp3I CGTCTC 2 cut(s) 515, 1211
FalI AAGNNNNNCTT 8 cut(s) 1324, 1356, 2121, 2153, 2358, 2390, 3398, 3430
FaqI GGGAC 7 cut(s) 3077, 3143, 3555, 3590, 3697, 3943, 4420
FauI CCCGC 4 cut(s) 81, 368, 2110, 3138
FauNDI CATATG 2 cut(s) 1658, 4370
FbaI TGATCA 2 cut(s) 1101, 3708
FblI GTMKAC 1 cut(s) 3791
FriOI GRGCYC 1 cut(s) 777
FspBI CTAG 8 cut(s) 945, 1010, 1022, 2885, 3228, 4220, 4290, 4299
GlaI GCGC 7 cut(s) 26, 97, 222, 252, 961, 1427, 2274
GsaI CCCAGC 1 cut(s) 17
GsuI CTGGAG 3 cut(s) 215, 3393, 4128
HaeII RGCGCY 5 cut(s) 28, 99, 224, 254, 963
HgaI GACGC 6 cut(s) 88, 105, 484, 2083, 3079, 3552
HhaI GCGC 7 cut(s) 27, 98, 223, 253, 962, 1428, 2275
Hin1I GRCGYC 4 cut(s) 80, 116, 2075, 3544
Hin6I GCGC 7 cut(s) 25, 96, 221, 251, 960, 1426, 2273
HinP1I GCGC 7 cut(s) 25, 96, 221, 251, 960, 1426, 2273
HincII GTYRAC 4 cut(s) 411, 652, 2058, 3780
HindII GTYRAC 4 cut(s) 411, 652, 2058, 3780
HindIII AAGCTT 5 cut(s) 631, 734, 1774, 2916, 4211
HpaI GTTAAC 1 cut(s) 3780
Hpy166II GTNNAC 9 cut(s) 411, 652, 1415, 2058, 3500, 3529, 3780, 3792, 4264
Hpy8I GTNNAC 9 cut(s) 411, 652, 1415, 2058, 3500, 3529, 3780, 3792, 4264
HpyAV CCTTC 9 cut(s) 455, 618, 1037, 1397, 1612, 1972, 3228, 3317, 3439
HpyCH4IV ACGT 6 cut(s) 601, 1167, 1450, 1510, 2509, 2587
HpySE526I ACGT 6 cut(s) 601, 1167, 1450, 1510, 2509, 2587
Hsp92I GRCGYC 4 cut(s) 80, 116, 2075, 3544
HspAI GCGC 7 cut(s) 25, 96, 221, 251, 960, 1426, 2273
Kpn2I TCCGGA 2 cut(s) 174, 2097
KroI GCCGGC 2 cut(s) 98, 197
KroNI GCCGGC 2 cut(s) 100, 199
Ksp22I TGATCA 2 cut(s) 1101, 3708
KspAI GTTAAC 1 cut(s) 3780
LmnI GCTCC 9 cut(s) 234, 283, 780, 1855, 2550, 3194, 3650, 4147, 4347
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 8 cut(s) 945, 1010, 1022, 2885, 3228, 4220, 4290, 4299
MaeII ACGT 6 cut(s) 601, 1167, 1450, 1510, 2509, 2587
MaeIII GTNAC 4 cut(s) 496, 573, 1393, 3267
MbiI CCGCTC 1 cut(s) 3921
MfeI CAATTG 3 cut(s) 1611, 1791, 3321
MflI RGATCY 6 cut(s) 36, 1229, 1940, 2005, 2747, 4175
MhlI GDGCHC 4 cut(s) 645, 777, 2531, 2983
MlsI TGGCCA 3 cut(s) 365, 3180, 3570
MluNI TGGCCA 3 cut(s) 365, 3180, 3570
MlyI GAGTC 6 cut(s) 137, 2027, 3311, 3719, 3977, 4114
MmeI TCCRAC 9 cut(s) 105, 682, 1333, 1856, 1869, 2456, 2870, 3535, 3628
Mox20I TGGCCA 3 cut(s) 365, 3180, 3570
Mph1103I ATGCAT 1 cut(s) 2818
MreI CGCCGGCG 1 cut(s) 197
MroI TCCGGA 2 cut(s) 174, 2097
MroNI GCCGGC 2 cut(s) 98, 197
MroXI GAANNNNTTC 3 cut(s) 813, 1777, 2658
MscI TGGCCA 3 cut(s) 365, 3180, 3570
MslI CAYNNNNRTG 3 cut(s) 404, 2958, 3978
Msp20I TGGCCA 3 cut(s) 365, 3180, 3570
MspA1I CMGCKG 2 cut(s) 241, 2621
MspCI CTTAAG 1 cut(s) 2681
MunI CAATTG 3 cut(s) 1611, 1791, 3321
Mva1269I GAATGC 3 cut(s) 1081, 1762, 4413
MvaI CCWGG 8 cut(s) 7, 215, 2550, 3186, 3372, 4276, 4324, 4419
MvnI CGCG 2 cut(s) 106, 4162
NaeI GCCGGC 2 cut(s) 100, 199
NciI CCSGG 4 cut(s) 415, 779, 4249, 4250
NdeI CATATG 2 cut(s) 1658, 4370
NgoMIV GCCGGC 2 cut(s) 98, 197
NlaIV GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1533, 3159, 3733, 4436
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 496
NsiI ATGCAT 1 cut(s) 2818
NspI RCATGY 4 cut(s) 664, 1140, 1684, 4031
NspV TTCGAA 1 cut(s) 2948
OliI CACNNNNGTG 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1684
PagI TCATGA 1 cut(s) 2959
PceI AGGCCT 1 cut(s) 4297
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 462
PctI GAATGC 3 cut(s) 1081, 1762, 4413
PdiI GCCGGC 2 cut(s) 100, 199
PdmI GAANNNNTTC 3 cut(s) 813, 1777, 2658
PfeI GAWTC 6 cut(s) 1378, 2160, 2950, 2969, 3147, 4286
PfoI TCCNGGA 1 cut(s) 4274
PleI GAGTC 6 cut(s) 136, 2026, 3311, 3719, 3977, 4114
PmaCI CACGTG 1 cut(s) 1451
PmlI CACGTG 1 cut(s) 1451
PpsI GAGTC 6 cut(s) 136, 2026, 3311, 3719, 3977, 4114
Ppu21I YACGTR 1 cut(s) 1451
PpuMI RGGWCCY 3 cut(s) 70, 3224, 4434
PsiI TTATAA 1 cut(s) 1902
Psp124BI GAGCTC 1 cut(s) 777
Psp1406I AACGTT 2 cut(s) 601, 2587
Psp5II RGGWCCY 3 cut(s) 70, 3224, 4434
Psp6I CCWGG 8 cut(s) 5, 213, 2548, 3184, 3370, 4274, 4322, 4417
PspCI CACGTG 1 cut(s) 1451
PspFI CCCAGC 1 cut(s) 13
PspGI CCWGG 8 cut(s) 5, 213, 2548, 3184, 3370, 4274, 4322, 4417
PspN4I GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1533, 3159, 3733, 4436
PspPPI RGGWCCY 3 cut(s) 70, 3224, 4434
PstI CTGCAG 4 cut(s) 1165, 1468, 2863, 3399
PsuI RGATCY 6 cut(s) 36, 1229, 1940, 2005, 2747, 4175
PvuII CAGCTG 2 cut(s) 241, 2621
RseI CAYNNNNRTG 3 cut(s) 404, 2958, 3978
SacI GAGCTC 1 cut(s) 777
ScaI AGTACT 1 cut(s) 4202
SchI GAGTC 6 cut(s) 137, 2027, 3311, 3719, 3977, 4114
SduI GDGCHC 4 cut(s) 645, 777, 2531, 2983
SexAI ACCWGGT 1 cut(s) 213
SfcI CTRYAG 5 cut(s) 1161, 1464, 2859, 3112, 3395
SfuI TTCGAA 1 cut(s) 2948
SgrAI CRCCGGYG 1 cut(s) 197
SmaI CCCGGG 1 cut(s) 4250
SmiMI CAYNNNNRTG 3 cut(s) 404, 2958, 3978
SphI GCATGC 1 cut(s) 1684
SseBI AGGCCT 1 cut(s) 4297
SspI AATATT 1 cut(s) 3469
SspMI CTAG 8 cut(s) 945, 1010, 1022, 2885, 3228, 4220, 4290, 4299
SstI GAGCTC 1 cut(s) 777
StuI AGGCCT 1 cut(s) 4297
StyI CCWWGG 5 cut(s) 1320, 1747, 2197, 2763, 3227
TaiI ACGT 6 cut(s) 604, 1170, 1453, 1513, 2512, 2590
TatI WGTACW 4 cut(s) 1933, 3677, 3757, 4200
TauI GCSGC 7 cut(s) 229, 265, 334, 473, 1665, 3869, 4382
TfiI GAWTC 6 cut(s) 1378, 2160, 2950, 2969, 3147, 4286
TseFI GTSAC 1 cut(s) 496
Tsp45I GTSAC 1 cut(s) 496
TspGWI ACGGA 2 cut(s) 497, 971
TspMI CCCGGG 1 cut(s) 4248
Vha464I CTTAAG 1 cut(s) 2681
XapI RAATTY 7 cut(s) 88, 809, 1357, 1599, 2668, 2740, 3274
XceI RCATGY 4 cut(s) 664, 1140, 1684, 4031
XcmI CCANNNNNNNNNTGG 1 cut(s) 2886
XmaI CCCGGG 1 cut(s) 4248
XmaJI CCTAGG 1 cut(s) 3227
XmiI GTMKAC 1 cut(s) 3791
XmnI GAANNNNTTC 3 cut(s) 813, 1777, 2658
XspI CTAG 8 cut(s) 945, 1010, 1022, 2885, 3228, 4220, 4290, 4299
ZrmI AGTACT 1 cut(s) 4202
Zsp2I ATGCAT 1 cut(s) 2818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.