MD10G1317800.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004422
Physical Location & Seq
Forward (+)
40086935 .. 40093503
6569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1317800.v1.1.491

Sequence Viewer

Length: 3972 bp
ATGTCTTGGGGACTGGGATGGAAGCGGCCCTCGGAGATCTTCCATCTCATGCTCAATTACGGCACCGAGGGTCCGCCGGAGACCTTCGACCGTACGTCTTCGGCTTCGTCTTCGTCCTCGTCGTCCTTGTCTTCCGTGGCGAATCAGGATCAGGAATTAGGGTTTCGGATCGACTTGGATTGGCAGGCTGGAGATGATGAGGAGCAGGTGGCTCTGAGGCTCAAGTCTCAGCTAATGGTGGCGTTGCCGATGCCTCAGGACACGGTGGTGGTTGAATTGAGGATCGAGGAAGCTGAGGAGGCTAATGTGGGTGTGGACATGAGAGTCGTGAGGCGCAGAGAGCCTTTGAGAGCCGTGACCATGACCAAGACGGTCGGCTCCAGCCAGCAAAGCGATGGCACTGGAGTCTTGACTCGTTTGTTGCGCTCCAATTTCGCTTCGACAATGCCTGCAGTTGCCGACGGCGAGGCGGCCTGCGGTGTGCATTGGCAGTGCGTCACCATCGTCAATCTGAGTGGTTGTGGGTTGTCGGTTTTACCAGTAGAGCTAACTCGACTGCCTCTTCTCGAAAAGCTGTACCTTGATAACAATAAGTTGTCACTTTTGCCTTCTGAGCTTGGCGAACTGAAAAGCTTAAAAGTCCTCAGGGTAGACTACAACATGCTGGTTTCAGTACCTTTAGAGCTAAGACAGTGTGTTGGATTAGTGGAACTTTCATTGGAACACAACAAGCTTGTACGGCCTTTGCTTGATTTCAGGGCTATGGCTGAGCTACGAGTTCTTAGGCTATTTGGAAACCCTCTTGAATTTCTTCCTGAAATCCTGCCTCTCCACAAACTTCATCACTTGTCTCTTGCGAATATCAGGATTGTGGCAGATGATAACTTGAGATCAGTGAATGTGCAAATAGAGATGGAAAATAGTTCCTATTTTGGTGCGTCTAGGCATAAGCTAAGCGCCTTTTTCTCTCTTATATTTCGGTTTTCTTCTTGTCATCACCCTTTACTAGCATCTGCACTTGCAAAGATTATGCAAGACCAAGGAAATCGTGCAGTCGTTGGCAAAGATGAGAATGCAGTGAGGCAGCTTATAAGTATGATAAGCAGTGACAACCGTCATGTGGTTGAACAAGCATGTTCTGCTCTTTCATCTCTTGCTGCAGATGTTTCAATTGCCATGCAGTTGATGAAATCCGACATTATGCAACCAATCGAGACAGTGTTGAAGTCTATGCCCCAGGGGGAAGTAATTTCTGTGTTGCAAGTTGTGGTGAAGCTGGCTTTTGCATCTGATGCTGTAGCTCAGAAGATGCTGACCAAGGATGTATTGAAATCTTTGAAATTATTGTGTGCCCACAAAACCCCTGAGGTACAAAGATTAGCTTTGTTGGCAGTAGGTAATTTGGCCTTCTGTTTGGAGAATCGCCGTATTATGGTTACTTCTGAAAGCTTGTGCGAACTTCTAATGCGCTTGACGGCTGCACCTGAGGCCCGTGTGCATAAAGCTGCGGCTCGTGCTTTGGCGATTCTCGGGGAGAATGGACTCCTGCGACGTGCCATAAGGGGGAGACCAGTGCCAAAGCAAGGACTGCGCATACTTTCAATGGATGGTGGTGGTATGAAAGGTCTGGCAACTGTACAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACAGATACATGAGTTGTTTGACCTCATATGCGGCACATCAACTGGTGGAATGCTTGCTGTTGCACTTGGCATTAAGCTAATGTCATTAAACAAATGTGAAGAAATATACAAAAATCTTGGAAAACATGTCTTTGCTGAACCTGCTCCAAAGGATAATGAAGCTGCAACTTGGAGAGAGAAGTTGGATCAGCTCTATAAAAGTTCGTCCCAGAGTTTTAGAGTTGTTGTACATGGATCTAAACATAGTGCAGATCAGTTTGAGAGATTGTTGAAGGAAATGTGTGCCGATGAGGATGGGGATCTGTTAATAGAGTCTGCAGTGAAAAACATTCCCAAAGTTTTTGTTGTATCAACTTTGGTGAGCGTGATGCCAGCTCAGCCTTTCCTATTCCGAAATTATCAGTATCCTGCGGGAACACTAGAAGTGCCTCCTGCAATTTCAGAGAGTTCTGGCATAACTGGACCACCTCCTGGATGTGCTGAAGGCTATAAGCGCAGTGCCTTTATAGGAACCTGTAAGCATCAAGTATGGCAAGCTATAAGAGCATCATCTGCTGCTCCGTATTATCTTGATGATTTCTCAGATGATGTGAATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTTCCATTAGAGAAGCACAACTTTTATGGCCAGACACAAAAATTGACTGCTTAGTTTCCATTGGCTGTGGTTCTGTTCCAACGAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGTCAGGTATTGATAGAAAGTGCATGCTCTGTGGAACGGGTGGAGGAAGCTTTGAGCACACTGCTACCCATGCTCCCTGGAATACAATATTTTCGGTTTAATCCAGTTGATGAACGCTGTGATATGGAACTGGATGAGACTGATCCTGCAGTCTGGATGAAATTGGAAGATGCAGTTGAGGAATATATTCAGAAAAATTCTCTTGTACTTAAGGATGCTTGTGAGAGACTACTTATGCCATTCCAACATGATGAGAAGTGGTCCGAGAATTTAAGATCTCAACATGTCCCTAAGTCAAAGGCATCAAGTGACGGGGAAAAAGGCCCATCTCTAGGTTGGAGGCGTAATGTACTACTTGTTGAAGCTTCACATAGCCCCAGTTCTGGTCGCACTCAGAACCATGCTCATGCACTTGAGTCATTTTGTGCTCGTAATGGAATACGATTATCGCTAATGCAAGGCATATCAGGATTTTTGAAAACAGTACCAGCAACAACATTCCCAACGCCATTTGCATCACCTTTATTTCCTCCAAGCATCCCATCAAGTCCACTTTTCTACAGTCCTGATTTTGGCCCACAGAGGGTTGGCCGAATCGATATGGTCCCACCTTTAAGCTTAGATGCGCAGTCTGGAAAAGGAGCTGTGTCACCACCTGAGTCTCCTTTAGGACCTAGACAGCTTTCTTTACCCGTCCAGTCATTGCACGAGAAGTTACAGAACTCGCCCCAAGTAGGCATTGTACATTTAGCCCTTCAAAATGACTCACTTGGCTCGATATTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAACCCGGAGAGCTTGCAGATAATTTTCTACAGAGTGTGAAACTAAGTTTGATATCAGTGATGCGAAACTGTCGCAGGAAGGCTGATTCATCGTTTGCCAATATTTCAACTATTTCTGATTTGGTTGCATGTAGACCATACTTCCAACTTGGAGGCATCGTTCACCGTTATATGGGACGCCAAACTCAAGTTATGGACGACGGCCAAGAAATTGGAGCATATTTGTTTCGTAGAACTGTCCCTTCTATCCATTTAACACCCGATGATGTTCGCTGGATGGTCGGAGCTTGGAGAGATAGGATCATTGTTTGCACAGGGACATATGGACCTACGCCACCACTGATTAAAGCATTTTTAGACTCTGGCGCTAAAGCTGTTATAAGTTCTTTAGCTCAGCCCCCGGAAACACAGCTGACAGCGCTCCACGGATCTGCAGAGTTCAGTGCTTTTGAAAACGGGAAGTTTGAGATTGGCGAGGAAGACGCTGAAGATGAAATCGAAGATGAGGAGGCTGAGCCTAGCAGTCCGCTGAGTGACTGGGAAGATAGTGAAAACGGGGACCCGTCTACAGGTTTTTGGGATGACGATGAGGAAGAGGTGTCGCAATTTGTTTGTCATTTATACGAGTCACTGTTTCGAGAGGGTGCGAGCGTGGATGCTGCTCTACGACAAGCTCTTGCCTCGCATCGGAAGTTGAGGTATTCATGCCATCTTCCCAGTGTACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1324

Amino Acids

145.7

Weight (kDa)

5.69

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 170 - 265 3.9e-06 Leucine-rich repeat region
Patatin PF01734 533 - 769 8.4e-20 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1091, 3623
AarI CACCTGC 1 cut(s) 196
AasI GACNNNNNNGTC 1 cut(s) 323
Acc16I TGCGCA 2 cut(s) 1592, 3057
Acc36I ACCTGC 2 cut(s) 196, 1822
AccB1I GGYRCC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 2144
AccI GTMKAC 3 cut(s) 651, 3376, 3809
AciI CCGC 8 cut(s) 25, 74, 470, 477, 1508, 1704, 2084, 3770
AclWI GGATC 9 cut(s) 156, 176, 290, 1866, 1915, 1980, 2567, 3551, 3679
AcoI YGGCCR 3 cut(s) 2339, 3019, 3445
AcsI RAATTY 4 cut(s) 806, 1641, 2626, 2698
AcuI CTGAAG 2 cut(s) 2175, 3750
AcyI GRCGYC 1 cut(s) 3421
AfeI AGCGCT 1 cut(s) 3663
AflII CTTAAG 1 cut(s) 2639
AflIII ACRYGT 2 cut(s) 1798, 2713
AhdI GACNNNNNGTC 1 cut(s) 94
AjiI CACGTC 1 cut(s) 1553
AjnI CCWGG 3 cut(s) 1236, 2143, 2506
AjuI GAANNNNNNNTTGG 2 cut(s) 2395, 2427
AleI CACNNNNGTG 1 cut(s) 266
AloI GAACNNNNNNTCC 2 cut(s) 3387, 3419
Alw21I GWGCWC 2 cut(s) 2489, 2860
Alw26I GTCTC 8 cut(s) 74, 231, 855, 1208, 1561, 2561, 2650, 3096
AlwI GGATC 9 cut(s) 156, 176, 290, 1866, 1915, 1980, 2567, 3551, 3679
Ama87I CYCGRG 1 cut(s) 1529
Aor51HI AGCGCT 1 cut(s) 3663
ApeKI GCWGC 7 cut(s) 1084, 1157, 1478, 1505, 1835, 2228, 3902
ApoI RAATTY 4 cut(s) 806, 1641, 2626, 2698
Asp700I GAANNNNTTC 2 cut(s) 810, 2616
AspLEI GCGC 9 cut(s) 336, 426, 959, 1470, 1593, 2169, 3058, 3611, 3664
AsuC2I CCSGG 2 cut(s) 3249, 3644
AsuHPI GGTGA 7 cut(s) 490, 989, 1282, 2044, 2940, 3072, 3398
AvaI CYCGRG 1 cut(s) 1529
AvaII GGWCC 7 cut(s) 71, 2135, 2691, 3034, 3101, 3569, 3802
AxyI CCTNAGG 4 cut(s) 255, 644, 1365, 1485
BaeGI GKGCMC 1 cut(s) 1354
BaeI ACNNNNGTAYC 2 cut(s) 1670, 1703
BalI TGGCCA 1 cut(s) 2341
BanI GGYRCC 1 cut(s) 62
BauI CACGAG 2 cut(s) 1512, 3137
BbsI GAAGAC 4 cut(s) 90, 102, 123, 3729
Bbv12I GWGCWC 2 cut(s) 2489, 2860
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 7 cut(s) 1096, 1144, 1465, 1492, 1822, 2215, 3889
BceAI ACGGC 7 cut(s) 76, 338, 478, 755, 1410, 1491, 3460
BciT130I CCWGG 3 cut(s) 1238, 2145, 2508
BciVI GTATCC 2 cut(s) 2088, 2419
BcnI CCSGG 2 cut(s) 3249, 3644
BcoDI GTCTC 8 cut(s) 74, 231, 855, 1208, 1561, 2561, 2650, 3096
BfaI CTAG 7 cut(s) 942, 1007, 2093, 2762, 3105, 3762, 3970
BfmI CTRYAG 9 cut(s) 450, 1158, 1296, 1989, 2577, 2989, 3272, 3675, 3810
BfoI RGCGCY 3 cut(s) 960, 3612, 3665
BfrI CTTAAG 1 cut(s) 2639
BfuAI ACCTGC 2 cut(s) 196, 1822
BfuI GTATCC 2 cut(s) 2088, 2419
BglII AGATCT 2 cut(s) 36, 2705
BlpI GCTNAGC 5 cut(s) 768, 953, 2049, 3636, 3756
Bme1390I CCNGG 5 cut(s) 1238, 2145, 2508, 3249, 3644
Bme18I GGWCC 7 cut(s) 71, 2135, 2691, 3034, 3101, 3569, 3802
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 1529
BmgBI CACGTC 1 cut(s) 1553
BmiI GGNNCC 7 cut(s) 64, 72, 379, 2185, 3036, 3803, 3804
BmrFI CCNGG 5 cut(s) 1238, 2145, 2508, 3249, 3644
BmrI ACTGGG 4 cut(s) 23, 2802, 3790, 3954
BmuI ACTGGG 4 cut(s) 23, 2802, 3790, 3954
BoxI GACNNNNGTC 1 cut(s) 1113
BpiI GAAGAC 4 cut(s) 90, 102, 123, 3729
BpmI CTGGAG 3 cut(s) 210, 364, 423
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 5 cut(s) 768, 953, 2049, 3636, 3756
BpuEI CTTGAG 4 cut(s) 206, 907, 2864, 3414
BpuMI CCSGG 2 cut(s) 3249, 3644
Bsa29I ATCGAT 1 cut(s) 3027
BsaBI GATNNNNATC 2 cut(s) 1971, 2265
BsaHI GRCGYC 1 cut(s) 3421
BsaI GGTCTC 2 cut(s) 74, 1561
BsaXI ACNNNNNCTCC 4 cut(s) 2487, 2517, 3387, 3417
Bse21I CCTNAGG 4 cut(s) 255, 644, 1365, 1485
Bse3DI GCAATG 1 cut(s) 3131
Bse8I GATNNNNATC 2 cut(s) 1971, 2265
BseBI CCWGG 3 cut(s) 1238, 2145, 2508
BseCI ATCGAT 1 cut(s) 3027
BseJI GATNNNNATC 2 cut(s) 1971, 2265
BseMI GCAATG 1 cut(s) 3131
BseRI GAGGAG 3 cut(s) 215, 311, 3764
BseSI GKGCMC 1 cut(s) 1354
BseXI GCAGC 7 cut(s) 1096, 1144, 1465, 1492, 1822, 2215, 3889
BsgI GTGCAG 4 cut(s) 999, 1071, 1464, 1941
Bsh1285I CGRYCG 2 cut(s) 91, 375
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 3027
BsiEI CGRYCG 2 cut(s) 91, 375
BsiHKAI GWGCWC 2 cut(s) 2489, 2860
BsiHKCI CYCGRG 1 cut(s) 1529
BsiSI CCGG 3 cut(s) 77, 3249, 3644
BsiWI CGTACG 1 cut(s) 92
BslFI GGGAC 8 cut(s) 24, 1864, 2702, 3020, 3432, 3467, 3574, 3815
BsmAI GTCTC 8 cut(s) 74, 231, 855, 1208, 1561, 2561, 2650, 3096
BsmFI GGGAC 8 cut(s) 24, 1864, 2702, 3020, 3432, 3467, 3574, 3815
BsmI GAATGC 2 cut(s) 1078, 1728
Bso31I GGTCTC 2 cut(s) 74, 1561
BsoBI CYCGRG 1 cut(s) 1529
Bsp1286I GDGCHC 3 cut(s) 1354, 2489, 2860
Bsp1407I TGTACA 4 cut(s) 1636, 1900, 3172, 3964
Bsp1720I GCTNAGC 5 cut(s) 768, 953, 2049, 3636, 3756
BspACI CCGC 8 cut(s) 25, 74, 470, 477, 1508, 1704, 2084, 3770
BspDI ATCGAT 1 cut(s) 3027
BspLI GGNNCC 7 cut(s) 64, 72, 379, 2185, 3036, 3803, 3804
BspMAI CTGCAG 5 cut(s) 454, 1162, 1993, 2581, 3679
BspMI ACCTGC 2 cut(s) 196, 1822
BspPI GGATC 9 cut(s) 156, 176, 290, 1866, 1915, 1980, 2567, 3551, 3679
BspT107I GGYRCC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 2639
BspTNI GGTCTC 2 cut(s) 74, 1561
BsrDI GCAATG 1 cut(s) 3131
BsrGI TGTACA 4 cut(s) 1636, 1900, 3172, 3964
BssNI GRCGYC 1 cut(s) 3421
BssSI CACGAG 2 cut(s) 1512, 3137
BssT1I CCWWGG 2 cut(s) 1039, 1317
Bst2BI CACGAG 2 cut(s) 1512, 3137
Bst2UI CCWGG 3 cut(s) 1238, 2145, 2508
Bst6I CTCTTC 2 cut(s) 567, 3831
BstACI GRCGYC 1 cut(s) 3421
BstAFI CTTAAG 1 cut(s) 2639
BstAPI GCANNNNNTGC 4 cut(s) 1139, 1292, 1588, 2225
BstAUI TGTACA 4 cut(s) 1636, 1900, 3172, 3964
BstDSI CCRYGG 2 cut(s) 135, 3667
BstH2I RGCGCY 3 cut(s) 960, 3612, 3665
BstHHI GCGC 9 cut(s) 336, 426, 959, 1470, 1593, 2169, 3058, 3611, 3664
BstMAI GTCTC 8 cut(s) 74, 231, 855, 1208, 1561, 2561, 2650, 3096
BstMCI CGRYCG 2 cut(s) 91, 375
BstNI CCWGG 3 cut(s) 1238, 2145, 2508
BstNSI RCATGY 6 cut(s) 664, 1137, 1802, 2457, 2717, 3375
BstPAI GACNNNNGTC 1 cut(s) 1113
BstSCI CCNGG 5 cut(s) 1236, 2143, 2506, 3247, 3642
BstSFI CTRYAG 9 cut(s) 450, 1158, 1296, 1989, 2577, 2989, 3272, 3675, 3810
BstSLI GKGCMC 1 cut(s) 1354
BstV1I GCAGC 7 cut(s) 1096, 1144, 1465, 1492, 1822, 2215, 3889
BstV2I GAAGAC 4 cut(s) 90, 102, 123, 3729
BstX2I RGATCY 5 cut(s) 36, 1907, 1972, 2705, 3671
BstXI CCANNNNNNTGG 1 cut(s) 3455
BstYI RGATCY 5 cut(s) 36, 1907, 1972, 2705, 3671
Bsu15I ATCGAT 1 cut(s) 3027
Bsu36I CCTNAGG 4 cut(s) 255, 644, 1365, 1485
BsuI GTATCC 2 cut(s) 2088, 2419
BsuTUI ATCGAT 1 cut(s) 3027
BtgI CCRYGG 2 cut(s) 135, 3667
BtgZI GCGATG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 1553
BtsI GCAGTG 6 cut(s) 497, 1083, 1111, 1998, 2176, 2489
BveI ACCTGC 2 cut(s) 196, 1822
CfoI GCGC 9 cut(s) 336, 426, 959, 1470, 1593, 2169, 3058, 3611, 3664
ClaI ATCGAT 1 cut(s) 3027
CseI GACGC 4 cut(s) 484, 927, 3429, 3734
CspCI CAANNNNNGTGG 2 cut(s) 496, 531
DrdI GACNNNNNNGTC 1 cut(s) 323
DriI GACNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 3 cut(s) 2339, 3019, 3445
Eam1104I CTCTTC 2 cut(s) 567, 3831
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 2 cut(s) 567, 3831
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 2 cut(s) 1039, 1317
Eco31I GGTCTC 2 cut(s) 74, 1561
Eco32I GATATC 1 cut(s) 3297
Eco47I GGWCC 7 cut(s) 71, 2135, 2691, 3034, 3101, 3569, 3802
Eco47III AGCGCT 1 cut(s) 3663
Eco57I CTGAAG 2 cut(s) 2175, 3750
Eco81I CCTNAGG 4 cut(s) 255, 644, 1365, 1485
Eco88I CYCGRG 1 cut(s) 1529
EcoO109I RGGNCCY 2 cut(s) 3101, 3802
EcoRII CCWGG 3 cut(s) 1236, 2143, 2506
EcoRV GATATC 1 cut(s) 3297
EcoT14I CCWWGG 2 cut(s) 1039, 1317
ErhI CCWWGG 2 cut(s) 1039, 1317
FalI AAGNNNNNCTT 4 cut(s) 1366, 1398, 2316, 2348
FaqI GGGAC 8 cut(s) 24, 1864, 2702, 3020, 3432, 3467, 3574, 3815
FauI CCCGC 1 cut(s) 2077
FauNDI CATATG 2 cut(s) 1700, 3565
FblI GTMKAC 3 cut(s) 651, 3376, 3809
FspBI CTAG 7 cut(s) 942, 1007, 2093, 2762, 3105, 3762, 3970
FspI TGCGCA 2 cut(s) 1592, 3057
GlaI GCGC 9 cut(s) 335, 425, 958, 1469, 1592, 2168, 3057, 3610, 3663
GsuI CTGGAG 3 cut(s) 210, 364, 423
HaeII RGCGCY 3 cut(s) 960, 3612, 3665
HapII CCGG 3 cut(s) 77, 3249, 3644
HgaI GACGC 4 cut(s) 484, 927, 3429, 3734
HhaI GCGC 9 cut(s) 336, 426, 959, 1470, 1593, 2169, 3058, 3611, 3664
Hin1I GRCGYC 1 cut(s) 3421
Hin6I GCGC 9 cut(s) 334, 424, 957, 1468, 1591, 2167, 3056, 3609, 3662
HinP1I GCGC 9 cut(s) 334, 424, 957, 1468, 1591, 2167, 3056, 3609, 3662
HindIII AAGCTT 6 cut(s) 631, 731, 1447, 2478, 2793, 3046
HpaII CCGG 3 cut(s) 77, 3249, 3644
HphI GGTGA 7 cut(s) 490, 989, 1282, 2044, 2940, 3072, 3398
Hpy166II GTNNAC 8 cut(s) 316, 652, 2981, 3377, 3406, 3810, 3965, 3967
Hpy8I GTNNAC 8 cut(s) 316, 652, 2981, 3377, 3406, 3810, 3965, 3967
Hpy99I CGWCG 4 cut(s) 124, 464, 1554, 3446
HpyCH4IV ACGT 2 cut(s) 95, 1552
HpySE526I ACGT 2 cut(s) 95, 1552
Hsp92I GRCGYC 1 cut(s) 3421
HspAI GCGC 9 cut(s) 334, 424, 957, 1468, 1591, 2167, 3056, 3609, 3662
KflI GGGWCCC 1 cut(s) 3802
Lsp1109I GCAGC 7 cut(s) 1096, 1144, 1465, 1492, 1822, 2215, 3889
MaeI CTAG 7 cut(s) 942, 1007, 2093, 2762, 3105, 3762, 3970
MaeII ACGT 2 cut(s) 95, 1552
MfeI CAATTG 2 cut(s) 1170, 1653
MflI RGATCY 5 cut(s) 36, 1907, 1972, 2705, 3671
MhlI GDGCHC 3 cut(s) 1354, 2489, 2860
MlsI TGGCCA 1 cut(s) 2341
MluNI TGGCCA 1 cut(s) 2341
MmeI TCCRAC 8 cut(s) 679, 1218, 1836, 2414, 2698, 2747, 3412, 3505
Mox20I TGGCCA 1 cut(s) 2341
MroXI GAANNNNTTC 2 cut(s) 810, 2616
MscI TGGCCA 1 cut(s) 2341
MslI CAYNNNNRTG 2 cut(s) 266, 2835
Msp20I TGGCCA 1 cut(s) 2341
MspA1I CMGCKG 2 cut(s) 3655, 3772
MspCI CTTAAG 1 cut(s) 2639
MspI CCGG 3 cut(s) 77, 3249, 3644
MspR9I CCNGG 5 cut(s) 1238, 2145, 2508, 3249, 3644
MunI CAATTG 2 cut(s) 1170, 1653
Mva1269I GAATGC 2 cut(s) 1078, 1728
MvaI CCWGG 3 cut(s) 1238, 2145, 2508
NciI CCSGG 2 cut(s) 3249, 3644
NdeI CATATG 2 cut(s) 1700, 3565
NlaIV GGNNCC 7 cut(s) 64, 72, 379, 2185, 3036, 3803, 3804
NmuCI GTSAC 8 cut(s) 355, 496, 597, 1106, 2738, 3078, 3776, 3870
NsbI TGCGCA 2 cut(s) 1592, 3057
NspI RCATGY 6 cut(s) 664, 1137, 1802, 2457, 2717, 3375
OliI CACNNNNGTG 1 cut(s) 266
PaeI GCATGC 1 cut(s) 2457
PaqCI CACCTGC 1 cut(s) 196
PasI CCCWGGG 1 cut(s) 1237
PciI ACATGT 2 cut(s) 1798, 2713
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 2 cut(s) 1078, 1728
PdmI GAANNNNTTC 2 cut(s) 810, 2616
PfeI GAWTC 6 cut(s) 142, 1420, 1525, 2266, 3024, 3329
Pfl23II CGTACG 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 2144
PfoI TCCNGGA 1 cut(s) 2143
PpuMI RGGWCCY 2 cut(s) 3101, 3802
PscI ACATGT 2 cut(s) 1798, 2713
PshAI GACNNNNGTC 1 cut(s) 1113
PsiI TTATAA 2 cut(s) 1091, 3623
Psp5II RGGWCCY 2 cut(s) 3101, 3802
Psp6I CCWGG 3 cut(s) 1236, 2143, 2506
PspGI CCWGG 3 cut(s) 1236, 2143, 2506
PspLI CGTACG 1 cut(s) 92
PspN4I GGNNCC 7 cut(s) 64, 72, 379, 2185, 3036, 3803, 3804
PspPPI RGGWCCY 2 cut(s) 3101, 3802
PstI CTGCAG 5 cut(s) 454, 1162, 1993, 2581, 3679
PsuI RGATCY 5 cut(s) 36, 1907, 1972, 2705, 3671
PvuII CAGCTG 1 cut(s) 3655
RseI CAYNNNNRTG 2 cut(s) 266, 2835
ScrFI CCNGG 5 cut(s) 1238, 2145, 2508, 3249, 3644
SduI GDGCHC 3 cut(s) 1354, 2489, 2860
SfcI CTRYAG 9 cut(s) 450, 1158, 1296, 1989, 2577, 2989, 3272, 3675, 3810
SinI GGWCC 7 cut(s) 71, 2135, 2691, 3034, 3101, 3569, 3802
SmiMI CAYNNNNRTG 2 cut(s) 266, 2835
SmlI CTYRAG 5 cut(s) 221, 886, 2639, 2843, 3429
SmoI CTYRAG 5 cut(s) 221, 886, 2639, 2843, 3429
SphI GCATGC 1 cut(s) 2457
SsiI CCGC 8 cut(s) 25, 74, 470, 477, 1508, 1704, 2084, 3770
SspI AATATT 2 cut(s) 2519, 3346
SspMI CTAG 7 cut(s) 942, 1007, 2093, 2762, 3105, 3762, 3970
StyD4I CCNGG 5 cut(s) 1236, 2143, 2506, 3247, 3642
StyI CCWWGG 2 cut(s) 1039, 1317
TaiI ACGT 2 cut(s) 98, 1555
TatI WGTACW 6 cut(s) 1636, 1900, 2635, 2779, 3172, 3964
TauI GCSGC 4 cut(s) 28, 473, 1511, 1707
TfiI GAWTC 6 cut(s) 142, 1420, 1525, 2266, 3024, 3329
TseFI GTSAC 8 cut(s) 355, 496, 597, 1106, 2738, 3078, 3776, 3870
TseI GCWGC 7 cut(s) 1084, 1157, 1478, 1505, 1835, 2228, 3902
Tsp45I GTSAC 8 cut(s) 355, 496, 597, 1106, 2738, 3078, 3776, 3870
TspGWI ACGGA 3 cut(s) 124, 2223, 3684
Van91I CCANNNNNTGG 1 cut(s) 2144
Vha464I CTTAAG 1 cut(s) 2639
VpaK11BI GGWCC 7 cut(s) 71, 2135, 2691, 3034, 3101, 3569, 3802
XapI RAATTY 4 cut(s) 806, 1641, 2626, 2698
XceI RCATGY 6 cut(s) 664, 1137, 1802, 2457, 2717, 3375
XcmI CCANNNNNNNNNTGG 2 cut(s) 392, 2763
XmiI GTMKAC 3 cut(s) 651, 3376, 3809
XmnI GAANNNNTTC 2 cut(s) 810, 2616
XspI CTAG 7 cut(s) 942, 1007, 2093, 2762, 3105, 3762, 3970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.