Rorug04G0406200

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004422
Physical Location & Seq
Forward (+)
57010507 .. 57014161
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0406200.1

Sequence Viewer

Length: 1170 bp
ATGGAGTCGGAGTTGGGTTTACTAGAGCAATCAATCAATAGTCGTTACTCGCTTTGGGTCCAAGAAGCTCTGAACGAATTGCCGGACAGTTTTACGGTGACTGATCCTTATATTTCCGGCCAACCCATCGTTTTTGCGAGCAAAGGGTTCCTGAAAATGATGGGGTACTCGAAAACCGAGGTGATTGGGAGGAATGGGAGGATATTTCAAGGGCCAGGTACTTGTAGAAGGTCGGTTATGGAAATAAGGGAGGCCATTAGAGAAGAGAGAGGTATTCAGATTAATTTGGTGAATTATAGGAGGGATGGGACACCCTTCTGGATGCTGTTTCATATGTGCCCTGTGTTTAGCAAAGAGGATGGGAGGGTGATTCATTTTGTGGGAATTCAAGTTCCCATTTTGCGAAAACCAAGGAGGAGGAATGGGGTTGGTTTGGTGTATGAGGAGGAGAGCAGGGTGAATGAGAGTGTTTATGGGTCTTGTAGGAGAGAAGTGTGCTCTGATTCTTTGGTGGATTTGGGCAGTGCTTTGTCTCTGGAGTGTGCATTGGATGATGCTGATAGTAGAGGACTGGAGATTGAAGAGACGTGTGAGGCAAGTGAATTAGAGAAGCGAAGAGCTGCGTCTGCCATGAATAACATCTTGTCTGTGCTAACACATTATAGCGAGTTAACAGGCAGACTGGTGTGTGGAAAGAGATGCAACTTAACTGGGGTGGGCCTTCTCAGTTCATCCTTAAATATTTCTCTTGGTAGAATCAAACAAAGCTTTGTATTAACAGATCCGCACTTACCTGACATGCCTATAGTTTACGCAAGTGAGGCCTTCTTCAAATTGACAGGTTATGCCAGACACGAAGTCTTGGGACGCAATTGTAGATTTCTAAGTGGCGAGGATACGGATTCCTCAGCGATATATCGGATAAAGGAAAGCGTTCAGAATGAAAAAGCATGCACAGTACGTATCTTGAATTACAGGAAGGATAAGAGTTCATTTTGGAATCTTCTTCATGTATCACCAATTCGTAATGCTTCTGGCAAGATGGAAGAAGCCTGTAAGAACCAGGATGGGCATGGGCTAAGCCCTGAGATGAGGCAGCTTAGCGCCGTTGGAGCTGTCAGGATTGCAGTGAGGAGTTTATCAATGGGTGCCGGCACATCCAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

389

Amino Acids

43.42

Weight (kDa)

8.24

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 24 - 129 1e-05 PAS fold
PAS_9 PF13426 40 - 130 2e-16 PAS domain
PAS_3 PF08447 43 - 129 5.8e-06 PAS fold
PAS_9 PF13426 257 - 347 2.9e-16 PAS domain
PAS PF00989 269 - 350 1e-06 PAS fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1148
AciI CCGC 1 cut(s) 785
AclWI GGATC 2 cut(s) 98, 776
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 3 cut(s) 167, 220, 960
AflIII ACRYGT 1 cut(s) 587
AgsI TTSAA 5 cut(s) 209, 389, 581, 832, 970
AhdI GACNNNNNGTC 1 cut(s) 857
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 2 cut(s) 214, 1062
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 5 cut(s) 68, 620, 768, 1099, 1115
AluI AGCT 5 cut(s) 68, 620, 768, 1099, 1115
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 2 cut(s) 537, 578
AlwI GGATC 2 cut(s) 98, 776
AoxI GGCC 5 cut(s) 118, 212, 252, 718, 822
ApeKI GCWGC 2 cut(s) 620, 1096
ApoI RAATTY 1 cut(s) 384
AseI ATTAAT 1 cut(s) 282
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 1106
AspS9I GGNCC 3 cut(s) 58, 212, 718
AsuHPI GGTGA 6 cut(s) 109, 193, 301, 379, 469, 1008
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 1148
Bbv12I GWGCWC 1 cut(s) 500
BbvCI CCTCAGC 1 cut(s) 907
BbvI GCAGC 2 cut(s) 607, 1108
BccI CCATC 6 cut(s) 134, 154, 299, 353, 1036, 1061
BceAI ACGGC 1 cut(s) 1091
BciT130I CCWGG 2 cut(s) 216, 1064
BciVI GTATCC 1 cut(s) 889
BcoDI GTCTC 2 cut(s) 537, 578
BfaI CTAG 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 1107
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 2 cut(s) 621, 1097
BlpI GCTNAGC 2 cut(s) 1079, 1100
BlsI GCNGC 2 cut(s) 622, 1098
Bme1390I CCNGG 2 cut(s) 216, 1064
Bme18I GGWCC 1 cut(s) 58
BmeRI GACNNNNNGTC 1 cut(s) 857
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 3 cut(s) 58, 212, 718
BmiI GGNNCC 3 cut(s) 59, 149, 1150
BmrFI CCNGG 2 cut(s) 216, 1064
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 3 cut(s) 312, 544, 689
BmuI ACTGGG 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 557, 593
Bpu10I CCTNAGC 1 cut(s) 907
Bpu1102I GCTNAGC 2 cut(s) 1079, 1100
BsaAI YACGTR 1 cut(s) 962
BsaJI CCNNGG 2 cut(s) 177, 410
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 1151
Bse1I ACTGG 3 cut(s) 576, 687, 715
BseBI CCWGG 2 cut(s) 216, 1064
BseDI CCNNGG 2 cut(s) 177, 410
BseGI GGATG 7 cut(s) 310, 327, 364, 556, 731, 1072, 1157
BseMII CTCAG 3 cut(s) 739, 921, 1077
BseNI ACTGG 3 cut(s) 576, 687, 715
BseRI GAGGAG 4 cut(s) 430, 458, 461, 1147
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 2 cut(s) 607, 1108
BshFI GGCC 5 cut(s) 120, 214, 254, 720, 824
BshNI GGYRCC 1 cut(s) 1148
BsiHKAI GWGCWC 1 cut(s) 500
BsiSI CCGG 3 cut(s) 83, 117, 1152
BslFI GGGAC 2 cut(s) 322, 879
BsmAI GTCTC 2 cut(s) 537, 578
BsmBI CGTCTC 1 cut(s) 578
BsmFI GGGAC 2 cut(s) 322, 879
BsnI GGCC 5 cut(s) 120, 214, 254, 720, 824
Bsp1286I GDGCHC 2 cut(s) 341, 500
Bsp143I GATC 2 cut(s) 103, 781
Bsp1720I GCTNAGC 2 cut(s) 1079, 1100
BspACI CCGC 1 cut(s) 785
BspANI GGCC 5 cut(s) 120, 214, 254, 720, 824
BspCNI CTCAG 3 cut(s) 738, 920, 1078
BspLI GGNNCC 3 cut(s) 59, 149, 1150
BspPI GGATC 2 cut(s) 98, 776
BspQI GCTCTTC 1 cut(s) 610
BspT107I GGYRCC 1 cut(s) 1148
BsrFI RCCGGY 1 cut(s) 1151
BsrI ACTGG 3 cut(s) 576, 687, 715
BssAI RCCGGY 1 cut(s) 1151
BssECI CCNNGG 2 cut(s) 177, 410
BssMI GATC 2 cut(s) 103, 781
BssT1I CCWWGG 1 cut(s) 410
Bst2UI CCWGG 2 cut(s) 216, 1064
Bst4CI ACNGT 3 cut(s) 89, 97, 958
Bst6I CTCTTC 3 cut(s) 258, 576, 610
BstBAI YACGTR 1 cut(s) 962
BstC8I GCNNGC 3 cut(s) 139, 952, 1153
BstDEI CTNAG 6 cut(s) 725, 884, 907, 1079, 1086, 1100
BstF5I GGATG 7 cut(s) 310, 327, 364, 556, 731, 1072, 1157
BstH2I RGCGCY 1 cut(s) 1107
BstHHI GCGC 1 cut(s) 1106
BstKTI GATC 2 cut(s) 106, 784
BstMAI GTCTC 2 cut(s) 537, 578
BstMBI GATC 2 cut(s) 103, 781
BstMWI GCNNNNNNNGC 3 cut(s) 626, 821, 1112
BstNI CCWGG 2 cut(s) 216, 1064
BstNSI RCATGY 2 cut(s) 802, 954
BstSCI CCNGG 2 cut(s) 214, 1062
BstSFI CTRYAG 1 cut(s) 804
BstSLI GKGCMC 1 cut(s) 341
BstSNI TACGTA 1 cut(s) 962
BstV1I GCAGC 2 cut(s) 607, 1108
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 5 cut(s) 120, 214, 254, 720, 824
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 7 cut(s) 310, 327, 364, 556, 731, 1072, 1157
BtsI GCAGTG 2 cut(s) 529, 1134
BtsIMutI CAGTG 2 cut(s) 529, 1134
Cac8I GCNNGC 3 cut(s) 139, 952, 1153
CfoI GCGC 1 cut(s) 1106
Cfr10I RCCGGY 1 cut(s) 1151
Cfr13I GGNCC 3 cut(s) 58, 212, 718
CseI GACGC 2 cut(s) 612, 876
Csp6I GTAC 3 cut(s) 166, 219, 959
CviAII CATG 5 cut(s) 631, 799, 951, 1010, 1073
CviQI GTAC 3 cut(s) 166, 219, 959
DdeI CTNAG 6 cut(s) 725, 884, 907, 1079, 1086, 1100
DpnI GATC 2 cut(s) 105, 783
DpnII GATC 2 cut(s) 103, 781
DriI GACNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 3 cut(s) 258, 576, 610
Eam1105I GACNNNNNGTC 1 cut(s) 857
EarI CTCTTC 3 cut(s) 258, 576, 610
Eco105I TACGTA 1 cut(s) 962
Eco130I CCWWGG 1 cut(s) 410
Eco147I AGGCCT 1 cut(s) 824
Eco47I GGWCC 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 384
EcoRII CCWGG 2 cut(s) 214, 1062
EcoT14I CCWWGG 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 410
Esp3I CGTCTC 1 cut(s) 578
FaeI CATG 5 cut(s) 634, 802, 954, 1013, 1076
FaqI GGGAC 2 cut(s) 322, 879
FatI CATG 5 cut(s) 630, 798, 950, 1009, 1072
FauNDI CATATG 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 621, 1097
FokI GGATG 7 cut(s) 317, 334, 371, 563, 718, 1079, 1144
Fsp4HI GCNGC 2 cut(s) 621, 1097
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 1105
GluI GCNGC 2 cut(s) 621, 1097
GsuI CTGGAG 2 cut(s) 557, 593
HaeII RGCGCY 1 cut(s) 1107
HaeIII GGCC 5 cut(s) 120, 214, 254, 720, 824
HapII CCGG 3 cut(s) 83, 117, 1152
HgaI GACGC 2 cut(s) 612, 876
HhaI GCGC 1 cut(s) 1106
Hin1II CATG 5 cut(s) 634, 802, 954, 1013, 1076
Hin6I GCGC 1 cut(s) 1104
HinP1I GCGC 1 cut(s) 1104
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HindIII AAGCTT 1 cut(s) 766
HinfI GANTC 6 cut(s) 5, 370, 503, 756, 902, 1000
HpaI GTTAAC 1 cut(s) 672
HpaII CCGG 3 cut(s) 83, 117, 1152
HphI GGTGA 6 cut(s) 109, 193, 301, 379, 469, 1008
Hpy166II GTNNAC 3 cut(s) 20, 672, 811
Hpy188I TCNGA 6 cut(s) 10, 72, 279, 502, 921, 939
Hpy188III TCNNGA 6 cut(s) 151, 319, 536, 967, 1120, 1167
Hpy8I GTNNAC 3 cut(s) 20, 672, 811
HpyAV CCTTC 5 cut(s) 222, 325, 731, 835, 973
HpyCH4III ACNGT 3 cut(s) 89, 97, 958
HpyCH4IV ACGT 2 cut(s) 587, 961
HpyCH4V TGCA 4 cut(s) 545, 702, 954, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 626, 821, 1112
HpyF3I CTNAG 6 cut(s) 725, 884, 907, 1079, 1086, 1100
HpySE526I ACGT 2 cut(s) 587, 961
Hsp92II CATG 5 cut(s) 634, 802, 954, 1013, 1076
HspAI GCGC 1 cut(s) 1104
KroI GCCGGC 1 cut(s) 1151
KroNI GCCGGC 1 cut(s) 1153
KspAI GTTAAC 1 cut(s) 672
Kzo9I GATC 2 cut(s) 103, 781
LguI GCTCTTC 1 cut(s) 610
LmnI GCTCC 1 cut(s) 1112
Lsp1109I GCAGC 2 cut(s) 607, 1108
LweI GCATC 3 cut(s) 312, 544, 689
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 587, 961
MaeIII GTNAC 2 cut(s) 44, 97
MalI GATC 2 cut(s) 105, 783
MboI GATC 2 cut(s) 103, 781
MboII GAAGA 7 cut(s) 275, 593, 627, 820, 995, 998, 1058
MfeI CAATTG 1 cut(s) 871
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 341, 500
MluCI AATT 9 cut(s) 77, 283, 292, 384, 602, 833, 871, 970, 1020
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 1090
MroNI GCCGGC 1 cut(s) 1151
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 5 cut(s) 282, 671, 707, 737, 776
MspI CCGG 3 cut(s) 83, 117, 1152
MspR9I CCNGG 2 cut(s) 216, 1064
MunI CAATTG 1 cut(s) 871
MvaI CCWGG 2 cut(s) 216, 1064
MwoI GCNNNNNNNGC 3 cut(s) 626, 821, 1112
NaeI GCCGGC 1 cut(s) 1153
NdeI CATATG 1 cut(s) 333
NdeII GATC 2 cut(s) 103, 781
NgoMIV GCCGGC 1 cut(s) 1151
NlaIII CATG 5 cut(s) 634, 802, 954, 1013, 1076
NlaIV GGNNCC 3 cut(s) 59, 149, 1150
NmuCI GTSAC 1 cut(s) 97
NspI RCATGY 2 cut(s) 802, 954
PaeI GCATGC 1 cut(s) 954
PceI AGGCCT 1 cut(s) 824
PciSI GCTCTTC 1 cut(s) 610
PdiI GCCGGC 1 cut(s) 1153
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 5 cut(s) 370, 503, 756, 902, 1000
PkrI GCNGC 2 cut(s) 622, 1098
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Ppu21I YACGTR 1 cut(s) 962
PshBI ATTAAT 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 214, 1062
PspGI CCWGG 2 cut(s) 214, 1062
PspN4I GGNNCC 3 cut(s) 59, 149, 1150
PspPI GGNCC 3 cut(s) 58, 212, 718
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 3 cut(s) 167, 220, 960
RsaNI GTAC 3 cut(s) 166, 219, 959
SapI GCTCTTC 1 cut(s) 610
SaqAI TTAA 5 cut(s) 282, 671, 707, 737, 776
SatI GCNGC 2 cut(s) 621, 1097
Sau3AI GATC 2 cut(s) 103, 781
Sau96I GGNCC 3 cut(s) 58, 212, 718
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 216, 1064
SduI GDGCHC 2 cut(s) 341, 500
SfaNI GCATC 3 cut(s) 312, 544, 689
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 1 cut(s) 58
SnaBI TACGTA 1 cut(s) 962
SphI GCATGC 1 cut(s) 954
Sse9I AATT 9 cut(s) 77, 283, 292, 384, 602, 833, 871, 970, 1020
SseBI AGGCCT 1 cut(s) 824
SsiI CCGC 1 cut(s) 785
SspI AATATT 1 cut(s) 742
SspMI CTAG 1 cut(s) 23
StuI AGGCCT 1 cut(s) 824
StyD4I CCNGG 2 cut(s) 214, 1062
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 3 cut(s) 89, 97, 958
TaiI ACGT 2 cut(s) 590, 964
TaqI TCGA 1 cut(s) 170
TasI AATT 9 cut(s) 77, 283, 292, 384, 602, 833, 871, 970, 1020
TfiI GAWTC 5 cut(s) 370, 503, 756, 902, 1000
Tru1I TTAA 5 cut(s) 282, 671, 707, 737, 776
Tru9I TTAA 5 cut(s) 282, 671, 707, 737, 776
TscAI CASTG 2 cut(s) 529, 1134
TseFI GTSAC 1 cut(s) 97
TseI GCWGC 2 cut(s) 620, 1096
Tsp45I GTSAC 1 cut(s) 97
TspDTI ATGAA 7 cut(s) 320, 362, 647, 720, 957, 981, 998
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 2 cut(s) 529, 1134
VpaK11BI GGWCC 1 cut(s) 58
VspI ATTAAT 1 cut(s) 282
XapI RAATTY 1 cut(s) 384
XceI RCATGY 2 cut(s) 802, 954
XcmI CCANNNNNNNNNTGG 1 cut(s) 1070
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.