Rh5DG032200

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004422
Physical Location & Seq
Reverse (-)
2257897 .. 2266081
8185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG032200.1

Sequence Viewer

Length: 3204 bp
ATGGCTGAGCTCCGGGTACTTAGGCTATTTGGAAACCCTCTTGAATTTCTTCCTGAAATCTTGCCTCTGCACAAACTTCATCATTTATCTCTCGCAAATATCAGGATTGTGGCAGATGACAGCTTGAGATCAGTGAATGTGCAGATAGAGATGGAAAACAGTTCGTATTTTGGTGCATCTAGGCATAAGCTAAGTGCCTTTTTCGCCCTTATATTCCGTTTTTCTTCTTGTCATCACCCTTTACTAGCATCTGCACTAGCAAAGATAATGCAAGACGAAGGAAACCGTGCAGTCGTTGGTAAAGATGAGAATGCAGTGCGACAGCTTATTAGTATGATCAGTAGTGATGACCGTCATGTGGTTCAAGAAGCATGTTCTGCTCTTTCATCTCTTGCTGCAGACATTTCAGTTGCAATGCAGTTGATGAAAGCCGACATTATGCAACCCATTGAGACGGTACTGAGATCTGTTCTCCAAGAGGAAGTGATTTCTGTATTACAAGTTGTGGTGAAGCTGGCTTTTGCATCTGATGCCGTAGCTCAGAAAATGCTGACCAAGGATGTATTGAAGTCGTTAAAACTCTTATGTGCCCACAGAACCCCAGAGGTGCAAAGGTTAGCTTTGGTAGCAGTTGGAAATTTGGCGTTTTGTTTGGAGAATCGTCGCCTTCTGGTTACTTCTGAAAGTTTGTGTGAACTTCTCATGCGCTTAACAGTTGTGCCTGAACCACGTGTGAATAAAGCTGCAGCTCGTGCTTTGGCAATTCTTGGGGAAAATGGAAATCTACGACGTGCCATAAAGGGGAGACAGGTGCCAAAACAAGGATTACGCATACTCTCAATGGATGGTGGTGGTATGAAAGGTCTGGCAACTGTGCAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACCCATACATGAGTTGTTCGACCTCATATGTGGCACATCAACTGGTGGCATGCTTGCTGTTGCTCTTGGCATTAAGCTAATGTCATTAGATCAGTGTGAAGAAATATACAAAAACCTTGGAAAACTTGTCTTTGCTGAACCTGCTCCAAAGGACAATGAAGCTGCAAGTTGGAGAGAAAAGTTGGATCAGCTTTATAAAAGTTCGTCGCAGAGTTTCAGAGTTGTTGTACATGGATCTAAACATAGTGCAGATCAGTTTGAGACATTGTTGAAGGAAATGTGTGCTGATGAGGATGGAGATCTGATGATAGAGTCGGCAGTGAAAAACATTCCCAAAGTTTTTGTAGTGTCAACTTTGGTGAGCATGACGCCAGCTCAGCCGTTCTTATTCCGGAATTATCAGTACCCTGCGGGAACGCCAGAAGTAGCTCTTGTAGTTTCAGAGAGTTCAGGAATCACTGTGCAAGAACCAACTACTTTGGGTACTGACCTTGGCTATAAACGCAGTGCTTTTATGGGAAGTTGTAAGCATCAAATATGGCAAGCTATAAGAGCATCATCTGCGGCGCCATACTATCTTGATGATTTCTCAGATGATATTCATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTGCCATCAGAGAAGCACAACTTTTATGGCCTGACACGAAAATTGATTGCTTAGTTTCTGTTGGCTGTGGTTCTGTTCCGACAAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGGCAAGTATTGATAGAGAGTTCTTGCTCTGTGGAACGCGTGGAGGAAGCCTTGAGCACATTGCTACCCATGCTCCCTGGAATACAATACTTTCGGTTTAATCCAGTTGATGAACGTTGTGATATGGAACTGGATGAGACTGATCCAGCTGTCTGGCTGAAATTGGAAGCCTCAGTTGAGGAATATATTCAGAAAAATTCTCTTACTCTTAAGGATGCCTGTGAGAGACTACTGCTGCCATTCCAAAACGACGAAAAGTGGTCCGAGAATTTGAGATCCCAACATTTCCCCAAGGCAAATGAGGTAGATGTGAAAAGCCCATCTCTAGGTTGGAGGCGTAATGTGCTTCTTGTTGAAGCTTCTCATAGCCCCAATTCTGGTCGAGCCTCGAATCACGCTCATGAACTTGAATCATTTTGTGCTCGTAATGGAATACGGGTATCTTTAATGCAAGGAATATCAGGGTTTGTGAAAACCGCGGCAGCAACAACATTTCCAACACCATTTCAGTCGCCCTTGTTTCCTGCAAGCGTCCCATCAAGCCCACTTTTCTACAGTCCTGACTTTGGCCCGCAGAGGGTTGGCCAAATTGATATGGTCCCACCTCTGAGCTTAGATGGCCAACCTGGAAAGGGCGCTGCCTCACCACCAAAGTCTCCTTCAGGACCTAGGCAGCTTTCTGCACCCGTTCAGTCATTGCATGAGAAGTTACAAAATTCGCCACAAGTGGGCATTGTGCATTTGGCCCTTCAAAATGACTCAATTGGCTCGATTTTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAACCTGGAGAACTTGCAGATAACTTTCTGCAGAGTGTTAAGTTGAGCTTGTTATCAGTTATGCGAAACCATCGCAAGAAGGCTGGATCACCTCTTTCCAATATTTCAACTGTTTCTGATTTGGTTGCTTGTAAACCAATCTTCCAACTTGGATGTATCGTTCACCGTTATATGGGACGCCAAACTCAAGTAATGGAAGATGGCCAAGAAATTGGGGCGTATTTGTTTCGTAGAACTGTCCCCTCTATTCACTTGACACCTGATGATGTTCGATGGATGGTTGGAGCTTGGAGAGACAGGATCATTATTTGTACAGGGACAAATGGGCCTACGCCAACTATGATCAAAGCATTTTTAGACTCTGGTGCCAAAGCGGTTATATCTTCCTCAGTACAGCCCCAAGAAACACAGTTAACATTCGGGTCTACTGATTTCAGTGTTCTTGAAAAGTTTGAGATTGGTGATGAGGAGGCTGAAGATGAAGAAGTTGAAGATGAGGCGGCTGAACCTGAAAGTCCGGTGAGTGATTGGGAAGACAGTGAAAATGGAGACCGCTCTATCGGCTTTGGGGATGATGATGAGGAAGAAGTGTCCCAATTCGTCTGTCATTTGTATGACTCACTGTTTCGAGAGGGTACGAGTGTGGATGTTGCTTTACGACATGCTCTTGCTCCACATCGGAAGTTGAGGTATTCATGTCATCTTCCCAGTATACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1067

Amino Acids

117.5

Weight (kDa)

5.59

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 279 - 521 4.2e-20 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1107
Acc36I ACCTGC 1 cut(s) 1060
AccB1I GGYRCC 3 cut(s) 811, 1477, 2849
AccBSI CCGCTC 1 cut(s) 3039
AccI GTMKAC 2 cut(s) 2909, 3196
AccII CGCG 2 cut(s) 1716, 2156
AccIII TCCGGA 1 cut(s) 1302
AciI CCGC 8 cut(s) 1322, 1475, 2154, 2156, 2249, 2858, 2984, 3037
AclI AACGTT 1 cut(s) 1792
AclWI GGATC 6 cut(s) 1104, 1153, 1814, 1947, 2578, 2792
AcoI YGGCCR 3 cut(s) 2260, 2296, 2686
AcsI RAATTY 6 cut(s) 44, 637, 879, 1873, 1945, 2392
AcuI CTGAAG 2 cut(s) 2322, 2979
AcvI CACGTG 1 cut(s) 731
AcyI GRCGYC 3 cut(s) 1280, 1478, 2662
AfaI GTAC 8 cut(s) 18, 459, 1140, 1316, 1396, 2797, 2877, 3121
AflII CTTAAG 1 cut(s) 1886
AflIII ACRYGT 2 cut(s) 730, 1714
AjiI CACGTC 1 cut(s) 791
AjnI CCWGG 3 cut(s) 1753, 2302, 2488
AjuI GAANNNNNNNTTGG 4 cut(s) 1642, 1674, 2320, 2352
AloI GAACNNNNNNTCC 2 cut(s) 2628, 2660
Alw21I GWGCWC 3 cut(s) 12, 1736, 2101
Alw26I GTCTC 8 cut(s) 446, 799, 1166, 1808, 1897, 2337, 2772, 3027
AlwI GGATC 6 cut(s) 1104, 1153, 1814, 1947, 2578, 2792
Aor13HI TCCGGA 1 cut(s) 1302
AoxI GGCC 7 cut(s) 1586, 2245, 2260, 2296, 2421, 2686, 2810
ApeKI GCWGC 8 cut(s) 395, 743, 746, 1073, 1912, 2159, 2315, 2350
ApoI RAATTY 6 cut(s) 44, 637, 879, 1873, 1945, 2392
Asp700I GAANNNNTTC 2 cut(s) 48, 1863
AspA2I CCTAGG 1 cut(s) 2345
AspLEI GCGC 3 cut(s) 708, 1480, 2315
AspS9I GGNCC 6 cut(s) 1938, 2246, 2275, 2342, 2422, 2810
AsuC2I CCSGG 1 cut(s) 14
AsuHPI GGTGA 8 cut(s) 227, 520, 1282, 2313, 2565, 2639, 2957, 3016
AvaII GGWCC 3 cut(s) 1938, 2275, 2342
AvrII CCTAGG 1 cut(s) 2345
BaeGI GKGCMC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 3111, 3144
BalI TGGCCA 3 cut(s) 2262, 2298, 2688
BanI GGYRCC 3 cut(s) 811, 1477, 2849
BanII GRGCYC 1 cut(s) 12
BauI CACGAG 1 cut(s) 750
BbrPI CACGTG 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 3024
Bbv12I GWGCWC 3 cut(s) 12, 1736, 2101
BbvI GCAGC 8 cut(s) 382, 730, 758, 1060, 1899, 2171, 2302, 2362
BceAI ACGGC 2 cut(s) 518, 1276
BcgI CGANNNNNNTGC 2 cut(s) 1627, 1661
BciT130I CCWGG 3 cut(s) 1755, 2304, 2490
BciVI GTATCC 1 cut(s) 1666
BclI TGATCA 2 cut(s) 336, 2826
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 8 cut(s) 446, 799, 1166, 1808, 1897, 2337, 2772, 3027
BfaI CTAG 5 cut(s) 180, 245, 257, 2003, 2346
BfmI CTRYAG 4 cut(s) 396, 744, 2230, 2513
BfoI RGCGCY 2 cut(s) 1481, 2316
BfrI CTTAAG 1 cut(s) 1886
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 1666
BglII AGATCT 2 cut(s) 464, 1210
BlnI CCTAGG 1 cut(s) 2345
BlpI GCTNAGC 2 cut(s) 6, 1287
Bme1390I CCNGG 4 cut(s) 14, 1755, 2304, 2490
Bme18I GGWCC 3 cut(s) 1938, 2275, 2342
BmgBI CACGTC 1 cut(s) 791
BmgT120I GGNCC 6 cut(s) 1938, 2246, 2275, 2342, 2422, 2810
BmiI GGNNCC 4 cut(s) 813, 1479, 2277, 2851
BmrFI CCNGG 4 cut(s) 14, 1755, 2304, 2490
BmrI ACTGGG 2 cut(s) 1686, 3186
BmsI GCATC 7 cut(s) 185, 257, 520, 533, 1450, 1475, 1882
BmuI ACTGGG 2 cut(s) 1686, 3186
BpiI GAAGAC 1 cut(s) 3024
BpmI CTGGAG 1 cut(s) 2511
Bpu1102I GCTNAGC 2 cut(s) 6, 1287
BpuEI CTTGAG 3 cut(s) 145, 1750, 2655
BpuMI CCSGG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 731
BsaBI GATNNNNATC 2 cut(s) 1209, 1512
BsaHI GRCGYC 3 cut(s) 1280, 1478, 2662
BsaI GGTCTC 1 cut(s) 3027
BsaJI CCNNGG 7 cut(s) 555, 1027, 1402, 1753, 1968, 2154, 2345
BsaWI WCCGGW 2 cut(s) 1302, 3001
BsaXI ACNNNNNCTCC 2 cut(s) 1734, 1764
Bse1I ACTGG 6 cut(s) 910, 958, 1681, 1781, 1812, 3192
Bse3DI GCAATG 3 cut(s) 420, 1736, 2372
Bse8I GATNNNNATC 2 cut(s) 1209, 1512
BseAI TCCGGA 1 cut(s) 1302
BseBI CCWGG 3 cut(s) 1755, 2304, 2490
BseDI CCNNGG 7 cut(s) 555, 1027, 1402, 1753, 1968, 2154, 2345
BseGI GGATG 9 cut(s) 565, 850, 1210, 1816, 1897, 2642, 2766, 3061, 3136
BseJI GATNNNNATC 2 cut(s) 1209, 1512
BseMI GCAATG 3 cut(s) 420, 1736, 2372
BseMII CTCAG 7 cut(s) 452, 554, 1301, 1515, 1863, 2276, 2886
BseNI ACTGG 6 cut(s) 910, 958, 1681, 1781, 1812, 3192
BseRI GAGGAG 1 cut(s) 2966
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 8 cut(s) 382, 730, 758, 1060, 1899, 2171, 2302, 2362
BsgI GTGCAG 6 cut(s) 53, 161, 237, 309, 1179, 2343
Bsh1236I CGCG 2 cut(s) 1716, 2156
BshFI GGCC 7 cut(s) 1588, 2247, 2262, 2298, 2423, 2688, 2812
BshNI GGYRCC 3 cut(s) 811, 1477, 2849
BsiHKAI GWGCWC 3 cut(s) 12, 1736, 2101
BsiSI CCGG 3 cut(s) 13, 1303, 3002
BslFI GGGAC 6 cut(s) 2195, 2261, 2673, 2708, 2815, 3061
BsmAI GTCTC 8 cut(s) 446, 799, 1166, 1808, 1897, 2337, 2772, 3027
BsmBI CGTCTC 1 cut(s) 446
BsmFI GGGAC 6 cut(s) 2195, 2261, 2673, 2708, 2815, 3061
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 7 cut(s) 1588, 2247, 2262, 2298, 2423, 2688, 2812
Bso31I GGTCTC 1 cut(s) 3027
Bsp1286I GDGCHC 4 cut(s) 12, 592, 1736, 2101
Bsp13I TCCGGA 1 cut(s) 1302
Bsp1407I TGTACA 2 cut(s) 1138, 2795
Bsp1720I GCTNAGC 2 cut(s) 6, 1287
BspACI CCGC 8 cut(s) 1322, 1475, 2154, 2156, 2249, 2858, 2984, 3037
BspANI GGCC 7 cut(s) 1588, 2247, 2262, 2298, 2423, 2688, 2812
BspCNI CTCAG 7 cut(s) 453, 553, 1300, 1514, 1862, 2277, 2885
BspEI TCCGGA 1 cut(s) 1302
BspFNI CGCG 2 cut(s) 1716, 2156
BspHI TCATGA 1 cut(s) 2077
BspLI GGNNCC 4 cut(s) 813, 1479, 2277, 2851
BspMAI CTGCAG 3 cut(s) 400, 748, 2517
BspMI ACCTGC 1 cut(s) 1060
BspPI GGATC 6 cut(s) 1104, 1153, 1814, 1947, 2578, 2792
BspT107I GGYRCC 3 cut(s) 811, 1477, 2849
BspTI CTTAAG 1 cut(s) 1886
BspTNI GGTCTC 1 cut(s) 3027
BsrBI CCGCTC 1 cut(s) 3039
BsrDI GCAATG 3 cut(s) 420, 1736, 2372
BsrGI TGTACA 2 cut(s) 1138, 2795
BsrI ACTGG 6 cut(s) 910, 958, 1681, 1781, 1812, 3192
BssECI CCNNGG 7 cut(s) 555, 1027, 1402, 1753, 1968, 2154, 2345
BssNAI GTATAC 1 cut(s) 3197
BssNI GRCGYC 3 cut(s) 1280, 1478, 2662
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 5 cut(s) 555, 1027, 1402, 1968, 2345
Bst1107I GTATAC 1 cut(s) 3197
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 3 cut(s) 1755, 2304, 2490
BstACI GRCGYC 3 cut(s) 1280, 1478, 2662
BstAFI CTTAAG 1 cut(s) 1886
BstAPI GCANNNNNTGC 4 cut(s) 377, 530, 752, 1472
BstAUI TGTACA 2 cut(s) 1138, 2795
BstBAI YACGTR 1 cut(s) 731
BstC8I GCNNGC 7 cut(s) 516, 962, 966, 1284, 1455, 2206, 2249
BstDSI CCRYGG 1 cut(s) 2154
BstF5I GGATG 9 cut(s) 565, 850, 1210, 1816, 1897, 2642, 2766, 3061, 3136
BstFNI CGCG 2 cut(s) 1716, 2156
BstH2I RGCGCY 2 cut(s) 1481, 2316
BstHHI GCGC 3 cut(s) 708, 1480, 2315
BstMAI GTCTC 8 cut(s) 446, 799, 1166, 1808, 1897, 2337, 2772, 3027
BstNI CCWGG 3 cut(s) 1755, 2304, 2490
BstNSI RCATGY 3 cut(s) 375, 964, 3149
BstSCI CCNGG 4 cut(s) 12, 1753, 2302, 2488
BstSFI CTRYAG 4 cut(s) 396, 744, 2230, 2513
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 2 cut(s) 1716, 2156
BstV1I GCAGC 8 cut(s) 382, 730, 758, 1060, 1899, 2171, 2302, 2362
BstV2I GAAGAC 1 cut(s) 3024
BstX2I RGATCY 4 cut(s) 464, 1145, 1210, 1952
BstXI CCANNNNNNTGG 2 cut(s) 1830, 2696
BstYI RGATCY 4 cut(s) 464, 1145, 1210, 1952
BstZ17I GTATAC 1 cut(s) 3197
BsuI GTATCC 1 cut(s) 1666
BsuRI GGCC 7 cut(s) 1588, 2247, 2262, 2298, 2423, 2688, 2812
BtgI CCRYGG 1 cut(s) 2154
BtgZI GCGATG 1 cut(s) 2540
BtrI CACGTC 1 cut(s) 791
BtsCI GGATG 9 cut(s) 565, 850, 1210, 1816, 1897, 2642, 2766, 3061, 3136
BtsI GCAGTG 3 cut(s) 321, 1236, 1423
BtsIMutI CAGTG 9 cut(s) 138, 321, 1010, 1236, 1368, 1423, 2926, 3028, 3104
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 7 cut(s) 516, 962, 966, 1284, 1455, 2206, 2249
CciI TCATGA 1 cut(s) 2077
CfoI GCGC 3 cut(s) 708, 1480, 2315
Cfr13I GGNCC 6 cut(s) 1938, 2246, 2275, 2342, 2422, 2810
Cfr42I CCGCGG 1 cut(s) 2157
CseI GACGC 3 cut(s) 1288, 2197, 2670
Csp6I GTAC 8 cut(s) 17, 458, 1139, 1315, 1395, 2796, 2876, 3120
CviQI GTAC 8 cut(s) 17, 458, 1139, 1315, 1395, 2796, 2876, 3120
DinI GGCGCC 1 cut(s) 1479
EaeI YGGCCR 3 cut(s) 2260, 2296, 2686
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 5 cut(s) 555, 1027, 1402, 1968, 2345
Eco24I GRGCYC 1 cut(s) 12
Eco31I GGTCTC 1 cut(s) 3027
Eco47I GGWCC 3 cut(s) 1938, 2275, 2342
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 2322, 2979
Eco72I CACGTG 1 cut(s) 731
EcoICRI GAGCTC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 2342
EcoRII CCWGG 3 cut(s) 1753, 2302, 2488
EcoT14I CCWWGG 5 cut(s) 555, 1027, 1402, 1968, 2345
EcoT38I GRGCYC 1 cut(s) 12
EgeI GGCGCC 1 cut(s) 1479
EheI GGCGCC 1 cut(s) 1479
ErhI CCWWGG 5 cut(s) 555, 1027, 1402, 1968, 2345
Esp3I CGTCTC 1 cut(s) 446
FalI AAGNNNNNCTT 8 cut(s) 604, 636, 1326, 1358, 1563, 1595, 2516, 2548
FaqI GGGAC 6 cut(s) 2195, 2261, 2673, 2708, 2815, 3061
FauI CCCGC 2 cut(s) 1315, 2256
FauNDI CATATG 1 cut(s) 938
FbaI TGATCA 2 cut(s) 336, 2826
FblI GTMKAC 2 cut(s) 2909, 3196
FokI GGATG 9 cut(s) 572, 857, 1217, 1823, 1904, 2649, 2773, 3068, 3143
FriOI GRGCYC 1 cut(s) 12
FspBI CTAG 5 cut(s) 180, 245, 257, 2003, 2346
GlaI GCGC 3 cut(s) 707, 1479, 2314
GsuI CTGGAG 1 cut(s) 2511
HaeII RGCGCY 2 cut(s) 1481, 2316
HaeIII GGCC 7 cut(s) 1588, 2247, 2262, 2298, 2423, 2688, 2812
HapII CCGG 3 cut(s) 13, 1303, 3002
HgaI GACGC 3 cut(s) 1288, 2197, 2670
HhaI GCGC 3 cut(s) 708, 1480, 2315
Hin1I GRCGYC 3 cut(s) 1280, 1478, 2662
Hin6I GCGC 3 cut(s) 706, 1478, 2313
HinP1I GCGC 3 cut(s) 706, 1478, 2313
HincII GTYRAC 2 cut(s) 1263, 2898
HindII GTYRAC 2 cut(s) 1263, 2898
HindIII AAGCTT 1 cut(s) 2034
HinfI GANTC 8 cut(s) 658, 1223, 1365, 2068, 2087, 2435, 2843, 3101
HpaI GTTAAC 1 cut(s) 2898
HpaII CCGG 3 cut(s) 13, 1303, 3002
HphI GGTGA 8 cut(s) 227, 520, 1282, 2313, 2565, 2639, 2957, 3016
Hpy166II GTNNAC 7 cut(s) 695, 1263, 2618, 2647, 2898, 2910, 3197
Hpy8I GTNNAC 7 cut(s) 695, 1263, 2618, 2647, 2898, 2910, 3197
Hpy99I CGWCG 4 cut(s) 666, 792, 1120, 1931
HpyAV CCTTC 7 cut(s) 272, 677, 892, 1177, 2346, 2435, 2557
HpyCH4IV ACGT 3 cut(s) 730, 790, 1792
HpySE526I ACGT 3 cut(s) 730, 790, 1792
Hsp92I GRCGYC 3 cut(s) 1280, 1478, 2662
HspAI GCGC 3 cut(s) 706, 1478, 2313
KasI GGCGCC 1 cut(s) 1477
Kpn2I TCCGGA 1 cut(s) 1302
Ksp22I TGATCA 2 cut(s) 336, 2826
KspAI GTTAAC 1 cut(s) 2898
KspI CCGCGG 1 cut(s) 2157
LmnI GCTCC 5 cut(s) 15, 1060, 1755, 2768, 3160
Lsp1109I GCAGC 8 cut(s) 382, 730, 758, 1060, 1899, 2171, 2302, 2362
LweI GCATC 7 cut(s) 185, 257, 520, 533, 1450, 1475, 1882
MaeI CTAG 5 cut(s) 180, 245, 257, 2003, 2346
MaeII ACGT 3 cut(s) 730, 790, 1792
MaeIII GTNAC 2 cut(s) 673, 2385
MbiI CCGCTC 1 cut(s) 3039
MfeI CAATTG 2 cut(s) 891, 2439
MflI RGATCY 4 cut(s) 464, 1145, 1210, 1952
MhlI GDGCHC 4 cut(s) 12, 592, 1736, 2101
MlsI TGGCCA 3 cut(s) 2262, 2298, 2688
MluI ACGCGT 1 cut(s) 1714
MluNI TGGCCA 3 cut(s) 2262, 2298, 2688
Mly113I GGCGCC 1 cut(s) 1478
MlyI GAGTC 4 cut(s) 1232, 2429, 2837, 3095
MmeI TCCRAC 8 cut(s) 613, 1061, 1074, 1661, 1988, 2198, 2653, 2746
Mox20I TGGCCA 3 cut(s) 2262, 2298, 2688
MroI TCCGGA 1 cut(s) 1302
MroXI GAANNNNTTC 2 cut(s) 48, 1863
MscI TGGCCA 3 cut(s) 2262, 2298, 2688
MslI CAYNNNNRTG 2 cut(s) 2076, 3096
Msp20I TGGCCA 3 cut(s) 2262, 2298, 2688
MspA1I CMGCKG 2 cut(s) 1826, 2156
MspCI CTTAAG 1 cut(s) 1886
MspI CCGG 3 cut(s) 13, 1303, 3002
MspR9I CCNGG 4 cut(s) 14, 1755, 2304, 2490
MunI CAATTG 2 cut(s) 891, 2439
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 3 cut(s) 1755, 2304, 2490
MvnI CGCG 2 cut(s) 1716, 2156
NarI GGCGCC 1 cut(s) 1478
NciI CCSGG 1 cut(s) 14
NdeI CATATG 1 cut(s) 938
NlaIV GGNNCC 4 cut(s) 813, 1479, 2277, 2851
NspI RCATGY 3 cut(s) 375, 964, 3149
PaeI GCATGC 1 cut(s) 964
PagI TCATGA 1 cut(s) 2077
PctI GAATGC 1 cut(s) 316
PdmI GAANNNNTTC 2 cut(s) 48, 1863
PfeI GAWTC 4 cut(s) 658, 1365, 2068, 2087
PleI GAGTC 4 cut(s) 1231, 2429, 2837, 3095
PluTI GGCGCC 1 cut(s) 1481
PmaCI CACGTG 1 cut(s) 731
PmlI CACGTG 1 cut(s) 731
PpsI GAGTC 4 cut(s) 1231, 2429, 2837, 3095
Ppu21I YACGTR 1 cut(s) 731
PpuMI RGGWCCY 1 cut(s) 2342
PsiI TTATAA 1 cut(s) 1107
Psp124BI GAGCTC 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 1792
Psp5II RGGWCCY 1 cut(s) 2342
Psp6I CCWGG 3 cut(s) 1753, 2302, 2488
PspCI CACGTG 1 cut(s) 731
PspGI CCWGG 3 cut(s) 1753, 2302, 2488
PspN4I GGNNCC 4 cut(s) 813, 1479, 2277, 2851
PspPI GGNCC 6 cut(s) 1938, 2246, 2275, 2342, 2422, 2810
PspPPI RGGWCCY 1 cut(s) 2342
PstI CTGCAG 3 cut(s) 400, 748, 2517
PsuI RGATCY 4 cut(s) 464, 1145, 1210, 1952
PvuII CAGCTG 1 cut(s) 1826
RsaI GTAC 8 cut(s) 18, 459, 1140, 1316, 1396, 2797, 2877, 3121
RsaNI GTAC 8 cut(s) 17, 458, 1139, 1315, 1395, 2796, 2876, 3120
RseI CAYNNNNRTG 2 cut(s) 2076, 3096
SacI GAGCTC 1 cut(s) 12
SacII CCGCGG 1 cut(s) 2157
Sau96I GGNCC 6 cut(s) 1938, 2246, 2275, 2342, 2422, 2810
SchI GAGTC 4 cut(s) 1232, 2429, 2837, 3095
ScrFI CCNGG 4 cut(s) 14, 1755, 2304, 2490
SduI GDGCHC 4 cut(s) 12, 592, 1736, 2101
SfaNI GCATC 7 cut(s) 185, 257, 520, 533, 1450, 1475, 1882
SfcI CTRYAG 4 cut(s) 396, 744, 2230, 2513
SfoI GGCGCC 1 cut(s) 1479
Sfr303I CCGCGG 1 cut(s) 2157
SgrBI CCGCGG 1 cut(s) 2157
SinI GGWCC 3 cut(s) 1938, 2275, 2342
SmiMI CAYNNNNRTG 2 cut(s) 2076, 3096
SmlI CTYRAG 4 cut(s) 124, 1729, 1886, 2670
SmoI CTYRAG 4 cut(s) 124, 1729, 1886, 2670
SphI GCATGC 1 cut(s) 964
SsiI CCGC 8 cut(s) 1322, 1475, 2154, 2156, 2249, 2858, 2984, 3037
SspDI GGCGCC 1 cut(s) 1477
SspI AATATT 1 cut(s) 2587
SspMI CTAG 5 cut(s) 180, 245, 257, 2003, 2346
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 4 cut(s) 12, 1753, 2302, 2488
StyI CCWWGG 5 cut(s) 555, 1027, 1402, 1968, 2345
TaiI ACGT 3 cut(s) 733, 793, 1795
TaqI TCGA 6 cut(s) 930, 2059, 2066, 2447, 2755, 3112
TatI WGTACW 3 cut(s) 1138, 2795, 2875
TauI GCSGC 3 cut(s) 1478, 2159, 2987
TfiI GAWTC 4 cut(s) 658, 1365, 2068, 2087
TscAI CASTG 9 cut(s) 138, 321, 1010, 1236, 1375, 1423, 2926, 3028, 3111
TseI GCWGC 8 cut(s) 395, 743, 746, 1073, 1912, 2159, 2315, 2350
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 9 cut(s) 138, 321, 1010, 1236, 1375, 1423, 2926, 3028, 3111
Vha464I CTTAAG 1 cut(s) 1886
VpaK11BI GGWCC 3 cut(s) 1938, 2275, 2342
XapI RAATTY 6 cut(s) 44, 637, 879, 1873, 1945, 2392
XceI RCATGY 3 cut(s) 375, 964, 3149
XcmI CCANNNNNNNNNTGG 1 cut(s) 2004
XmaJI CCTAGG 1 cut(s) 2345
XmiI GTMKAC 2 cut(s) 2909, 3196
XmnI GAANNNNTTC 2 cut(s) 48, 1863
XspI CTAG 5 cut(s) 180, 245, 257, 2003, 2346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.