pycom05g31230

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004422
Physical Location & Seq
Forward (+)
31060332 .. 31061708
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31230.1

Sequence Viewer

Length: 1089 bp
ATGCAAGGCATATCAGGATTTGTGAAAACGGTACCAGCAACAACGTTCCCAACTCCGTTTGCATCACCTTTATTTCCTCCAAGCATTCCATCAAGTCCACTTTTCTACAGTCCTGATTTTGGCCCACAGAGGGTTGGCCGAATCGATATGGTCCCACCTTTAAGCTTAGATGGACAGTCTGGAAAAGGAGCTGCATCACCACCTGATTCTCCTTCAGGACCTAGACAGCTTTCTTTACCCGTCCAGTCATTGCACGAGAAGTTACAGAACTCGCCCCAAGTGGGGATTGTACATTTAGCCCTTCAAAATGACTCTCTTGGCTCGATATTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAGCCTGGAGAGCTTGCAGATAAGTTTCTTCAGAGTGTTAAACTGAGTTTGATATCAGTGGTGCGAAACCGCCGCAGGAAGGCTGATTCATCTTTCGCCAATATTTCAACTATTTCTGATTTGGTTGCATGTAGACCGTACTTCCAAATTGGAGGCATCGTTCACCGTTATATGGGACGCCAAACTCAAGTTATGGACGATGGCCAAGAAATTGGAGCATATTTGTTTCGTAGAACTGTGCCTTCTATCCATTTAACACCTGATGATGTTCGCTGGATGGTCGGAGCTTGGAGAGATAGGATCATTATTTGCACAGGGACATATGGACCTACGCCACCACTGATTAAAGCATTTTTAGACTCTGGCGCTAAAGCTGTTATAAGTTCTTTAGCTCAGCCCCCAGAAACACAGCTGACAGCAGTCCATGGATCTGCAGAGTTCAGTGTTTTTGAAAACGGGAAGTTTGAGATTGGCGAGGAAGAGGCCGAAGACGAAGTCGAAGATGAGGAGGCTGAGCCTACCAGTCCGGTGAGTGACTGGGAAGATAGTGAAAACGGGGACCGGTCTACAGGTTTTTGGGATGACGATGAGGAAGAGGTGTCGCAATTTGTTTGTCAATTGTACGAGTCACTGTTTCGAGAGGGTGTGAGCGTGGATGTTGCTCTACGACAAGCTCTTGCCTCGCACCGGAAGTTGAGGTATTCATGCCATCTTCCCAGCATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

363

Amino Acids

39.74

Weight (kDa)

4.83

Isoelectric Point (pI)

61.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
Acc65I GGTACC 1 cut(s) 31
AccB1I GGYRCC 1 cut(s) 31
AccI GTMKAC 2 cut(s) 493, 926
AciI CCGC 2 cut(s) 430, 433
AclI AACGTT 1 cut(s) 44
AclWI GGATC 2 cut(s) 668, 796
AcoI YGGCCR 2 cut(s) 136, 562
AcuI CTGAAG 2 cut(s) 198, 374
AcyI GRCGYC 1 cut(s) 538
AfaI GTAC 4 cut(s) 33, 291, 500, 983
AfiI CCNNNNNNNGG 7 cut(s) 119, 130, 281, 282, 439, 532, 1047
AgeI ACCGGT 1 cut(s) 921
AgsI TTSAA 3 cut(s) 305, 468, 812
AjnI CCWGG 1 cut(s) 364
AloI GAACNNNNNNTCC 2 cut(s) 504, 536
AluBI AGCT 9 cut(s) 165, 191, 229, 373, 647, 734, 752, 772, 1034
AluI AGCT 9 cut(s) 165, 191, 229, 373, 647, 734, 752, 772, 1034
AlwI GGATC 2 cut(s) 668, 796
AoxI GGCC 4 cut(s) 121, 136, 562, 843
ApeKI GCWGC 1 cut(s) 191
ArsI GACNNNNNNTTYG 2 cut(s) 840, 872
AsiGI ACCGGT 1 cut(s) 921
Asp718I GGTACC 1 cut(s) 31
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 5 cut(s) 122, 151, 218, 686, 919
AsuHPI GGTGA 4 cut(s) 57, 189, 515, 901
AvaII GGWCC 4 cut(s) 151, 218, 686, 919
BalI TGGCCA 1 cut(s) 564
BanI GGYRCC 1 cut(s) 31
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 855
BbvI GCAGC 1 cut(s) 178
BccI CCATC 5 cut(s) 97, 164, 554, 631, 1077
BciT130I CCWGG 1 cut(s) 366
BfaI CTAG 2 cut(s) 222, 1087
BfmI CTRYAG 3 cut(s) 106, 792, 927
BfoI RGCGCY 1 cut(s) 729
BisI GCNGC 2 cut(s) 192, 433
BlpI GCTNAGC 3 cut(s) 360, 753, 873
BlsI GCNGC 2 cut(s) 193, 434
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 4 cut(s) 151, 218, 686, 919
BmgT120I GGNCC 5 cut(s) 122, 151, 218, 686, 919
BmiI GGNNCC 3 cut(s) 33, 153, 920
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 907
BmsI GCATC 3 cut(s) 71, 203, 525
BmuI ACTGGG 1 cut(s) 907
BoxI GACNNNNGTC 1 cut(s) 779
BpiI GAAGAC 1 cut(s) 855
BpmI CTGGAG 1 cut(s) 387
Bpu1102I GCTNAGC 3 cut(s) 360, 753, 873
BpuEI CTTGAG 1 cut(s) 531
Bsa29I ATCGAT 1 cut(s) 144
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 1 cut(s) 784
BsaWI WCCGGW 3 cut(s) 886, 921, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 7 cut(s) 119, 130, 281, 282, 439, 532, 1047
Bse118I RCCGGY 1 cut(s) 921
Bse1I ACTGG 3 cut(s) 244, 882, 902
Bse3DI GCAATG 1 cut(s) 248
BseBI CCWGG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 784
BseGI GGATG 3 cut(s) 642, 946, 1021
BseLI CCNNNNNNNGG 7 cut(s) 119, 130, 281, 282, 439, 532, 1047
BseMI GCAATG 1 cut(s) 248
BseMII CTCAG 4 cut(s) 351, 395, 767, 864
BseNI ACTGG 3 cut(s) 244, 882, 902
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 1076
BshFI GGCC 4 cut(s) 123, 138, 564, 845
BshNI GGYRCC 1 cut(s) 31
BshTI ACCGGT 1 cut(s) 921
BshVI ATCGAT 1 cut(s) 144
BsiSI CCGG 3 cut(s) 887, 922, 1048
BslFI GGGAC 4 cut(s) 137, 549, 691, 932
BslI CCNNNNNNNGG 7 cut(s) 119, 130, 281, 282, 439, 532, 1047
BsmFI GGGAC 4 cut(s) 137, 549, 691, 932
BsmI GAATGC 1 cut(s) 84
BsnI GGCC 4 cut(s) 123, 138, 564, 845
Bsp1407I TGTACA 1 cut(s) 289
Bsp143I GATC 2 cut(s) 660, 788
Bsp1720I GCTNAGC 3 cut(s) 360, 753, 873
Bsp19I CCATGG 1 cut(s) 784
BspACI CCGC 2 cut(s) 430, 433
BspANI GGCC 4 cut(s) 123, 138, 564, 845
BspCNI CTCAG 4 cut(s) 352, 396, 766, 865
BspDI ATCGAT 1 cut(s) 144
BspLI GGNNCC 3 cut(s) 33, 153, 920
BspMAI CTGCAG 1 cut(s) 796
BspPI GGATC 2 cut(s) 668, 796
BspT107I GGYRCC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 248
BsrFI RCCGGY 1 cut(s) 921
BsrGI TGTACA 1 cut(s) 289
BsrI ACTGG 3 cut(s) 244, 882, 902
BssAI RCCGGY 1 cut(s) 921
BssECI CCNNGG 1 cut(s) 784
BssMI GATC 2 cut(s) 660, 788
BssNI GRCGYC 1 cut(s) 538
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 784
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 7 cut(s) 31, 110, 177, 498, 527, 598, 993
Bst6I CTCTTC 2 cut(s) 834, 948
BstACI GRCGYC 1 cut(s) 538
BstAUI TGTACA 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 5 cut(s) 166, 360, 404, 753, 873
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 3 cut(s) 642, 946, 1021
BstH2I RGCGCY 1 cut(s) 729
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 2 cut(s) 663, 791
BstMBI GATC 2 cut(s) 660, 788
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 492
BstPAI GACNNNNGTC 1 cut(s) 779
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 3 cut(s) 106, 792, 927
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 788
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 1 cut(s) 788
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 4 cut(s) 123, 138, 564, 845
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 784
BtsCI GGATG 3 cut(s) 642, 946, 1021
BtsIMutI CAGTG 4 cut(s) 423, 698, 808, 989
Cac8I GCNNGC 1 cut(s) 375
CfoI GCGC 1 cut(s) 728
Cfr10I RCCGGY 1 cut(s) 921
Cfr13I GGNCC 5 cut(s) 122, 151, 218, 686, 919
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 1 cut(s) 546
Csp6I GTAC 4 cut(s) 32, 290, 499, 982
CspAI ACCGGT 1 cut(s) 921
CviAII CATG 3 cut(s) 489, 785, 1065
CviQI GTAC 4 cut(s) 32, 290, 499, 982
DdeI CTNAG 5 cut(s) 166, 360, 404, 753, 873
DpnI GATC 2 cut(s) 662, 790
DpnII GATC 2 cut(s) 660, 788
EaeI YGGCCR 2 cut(s) 136, 562
Eam1104I CTCTTC 2 cut(s) 834, 948
EarI CTCTTC 2 cut(s) 834, 948
Eco130I CCWWGG 1 cut(s) 784
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 4 cut(s) 151, 218, 686, 919
Eco57I CTGAAG 2 cut(s) 198, 374
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 364
EcoRV GATATC 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 784
ErhI CCWWGG 1 cut(s) 784
FaeI CATG 3 cut(s) 492, 788, 1068
FaqI GGGAC 4 cut(s) 137, 549, 691, 932
FatI CATG 3 cut(s) 488, 784, 1064
FauNDI CATATG 1 cut(s) 682
FblI GTMKAC 2 cut(s) 493, 926
Fnu4HI GCNGC 2 cut(s) 192, 433
FokI GGATG 3 cut(s) 649, 953, 1028
Fsp4HI GCNGC 2 cut(s) 192, 433
FspBI CTAG 2 cut(s) 222, 1087
GlaI GCGC 1 cut(s) 727
GluI GCNGC 2 cut(s) 192, 433
GsaI CCCAGC 1 cut(s) 1080
GsuI CTGGAG 1 cut(s) 387
HaeII RGCGCY 1 cut(s) 729
HaeIII GGCC 4 cut(s) 123, 138, 564, 845
HapII CCGG 3 cut(s) 887, 922, 1048
HgaI GACGC 1 cut(s) 546
HhaI GCGC 1 cut(s) 728
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 3 cut(s) 492, 788, 1068
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 6 cut(s) 141, 206, 311, 446, 719, 986
HpaII CCGG 3 cut(s) 887, 922, 1048
HphI GGTGA 4 cut(s) 57, 189, 515, 901
Hpy166II GTNNAC 4 cut(s) 98, 494, 523, 927
Hpy188I TCNGA 3 cut(s) 393, 478, 644
Hpy188III TCNNGA 5 cut(s) 15, 113, 180, 216, 998
Hpy8I GTNNAC 4 cut(s) 98, 494, 523, 927
HpyAV CCTTC 4 cut(s) 222, 311, 433, 612
HpyCH4III ACNGT 7 cut(s) 31, 110, 177, 498, 527, 598, 993
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 8 cut(s) 4, 62, 194, 253, 377, 488, 672, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 5 cut(s) 166, 360, 404, 753, 873
HpySE526I ACGT 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 3 cut(s) 492, 788, 1068
HspAI GCGC 1 cut(s) 726
KpnI GGTACC 1 cut(s) 35
Kzo9I GATC 2 cut(s) 660, 788
LmnI GCTCC 3 cut(s) 188, 575, 644
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 3 cut(s) 71, 203, 525
MaeI CTAG 2 cut(s) 222, 1087
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 3 cut(s) 261, 893, 987
MalI GATC 2 cut(s) 662, 790
MboI GATC 2 cut(s) 660, 788
MboII GAAGA 7 cut(s) 380, 851, 860, 872, 914, 965, 1064
MfeI CAATTG 1 cut(s) 977
MflI RGATCY 1 cut(s) 788
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 4 cut(s) 507, 570, 965, 977
MluNI TGGCCA 1 cut(s) 564
MlyI GAGTC 3 cut(s) 305, 713, 995
MmeI TCCRAC 1 cut(s) 622
Mox20I TGGCCA 1 cut(s) 564
MscI TGGCCA 1 cut(s) 564
MseI TTAA 5 cut(s) 161, 329, 399, 614, 705
Msp20I TGGCCA 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 772
MspI CCGG 3 cut(s) 887, 922, 1048
MspR9I CCNGG 1 cut(s) 366
MunI CAATTG 1 cut(s) 977
Mva1269I GAATGC 1 cut(s) 84
MvaI CCWGG 1 cut(s) 366
MwoI GCNNNNNNNGC 1 cut(s) 370
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 1 cut(s) 682
NdeII GATC 2 cut(s) 660, 788
NlaIII CATG 3 cut(s) 492, 788, 1068
NlaIV GGNNCC 3 cut(s) 33, 153, 920
NmuCI GTSAC 2 cut(s) 893, 987
NspI RCATGY 1 cut(s) 492
PctI GAATGC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 141, 206, 446
PflFI GACNNNGTC 1 cut(s) 854
PinAI ACCGGT 1 cut(s) 921
PkrI GCNGC 2 cut(s) 193, 434
PleI GAGTC 3 cut(s) 305, 713, 994
PpsI GAGTC 3 cut(s) 305, 713, 994
PpuMI RGGWCCY 1 cut(s) 218
PshAI GACNNNNGTC 1 cut(s) 779
PsiI TTATAA 1 cut(s) 740
Psp1406I AACGTT 1 cut(s) 44
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 1076
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 3 cut(s) 33, 153, 920
PspPI GGNCC 5 cut(s) 122, 151, 218, 686, 919
PspPPI RGGWCCY 1 cut(s) 218
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 1 cut(s) 788
PsyI GACNNNGTC 1 cut(s) 854
PvuII CAGCTG 1 cut(s) 772
RsaI GTAC 4 cut(s) 33, 291, 500, 983
RsaNI GTAC 4 cut(s) 32, 290, 499, 982
SaqAI TTAA 5 cut(s) 161, 329, 399, 614, 705
SatI GCNGC 2 cut(s) 192, 433
Sau3AI GATC 2 cut(s) 660, 788
Sau96I GGNCC 5 cut(s) 122, 151, 218, 686, 919
SchI GAGTC 3 cut(s) 305, 713, 995
ScrFI CCNGG 1 cut(s) 366
SfaNI GCATC 3 cut(s) 71, 203, 525
SfcI CTRYAG 3 cut(s) 106, 792, 927
SinI GGWCC 4 cut(s) 151, 218, 686, 919
SmlI CTYRAG 1 cut(s) 546
SmoI CTYRAG 1 cut(s) 546
Sse9I AATT 4 cut(s) 507, 570, 965, 977
SsiI CCGC 2 cut(s) 430, 433
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 222, 1087
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 784
TaaI ACNGT 7 cut(s) 31, 110, 177, 498, 527, 598, 993
TaiI ACGT 1 cut(s) 47
TaqI TCGA 4 cut(s) 144, 323, 858, 997
TasI AATT 4 cut(s) 507, 570, 965, 977
TatI WGTACW 1 cut(s) 289
TauI GCSGC 1 cut(s) 435
TfiI GAWTC 3 cut(s) 141, 206, 446
Tru1I TTAA 5 cut(s) 161, 329, 399, 614, 705
Tru9I TTAA 5 cut(s) 161, 329, 399, 614, 705
TscAI CASTG 4 cut(s) 423, 705, 808, 996
TseFI GTSAC 2 cut(s) 893, 987
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 2 cut(s) 893, 987
TspDTI ATGAA 2 cut(s) 438, 1053
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 4 cut(s) 423, 705, 808, 996
Tth111I GACNNNGTC 1 cut(s) 854
VpaK11BI GGWCC 4 cut(s) 151, 218, 686, 919
XceI RCATGY 1 cut(s) 492
XmiI GTMKAC 2 cut(s) 493, 926
XspI CTAG 2 cut(s) 222, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.