pycom10g26930

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004422
Physical Location & Seq
Forward (+)
28083322 .. 28084993
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g26930.1

Sequence Viewer

Length: 1308 bp
ATGCCATTCCAACATGATGAGAAGTGGTCCGAGAATTTAAGATCTCAACATGTCCCTAAGTCAAAGGCATCAAGTGACGGGGAAAAAGGCCCATCTCTAGGTTGGAGGCGTAATGTACTACTTGTTGAAGCTTCACATAGCCCCAATTCTGGTCGCACTCAGAACCATGCTCATGCACTTGAGTCATTTTGTGCTCGTAATGGAATACGATTATCGCTAATGCAAGGCATATCAGGATTTTTGAAAACAGTACCAGCAACAACATTCCCAACGCCATTTGCATCACCTTTATTTCCTCCAAGCATCCCATCAAGTCCACTTTTCTACAGTCCTGATTTTGGGCCACAGAGGGTTGGCCGAATCGATATGGTCCCACCTTTAAGCTTAGATGCGCAGTCTGGAAAAGGAGCTGTGTCACCACCTGAGTCTCCTTTAGGACCTAGACAGCTTTCTTTACCCGTCCAGTCATTGCACGAGAAGTTACAGAACTCGCCCCAAGTGGGCATTGTGCATTTAGCCCTTCAAAATGACTCACTTGGCTCAATATTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAACCCGGAGAGCTTGCAGATAATTTTCTTCAGAGTGTGAAACTGAGTTTGATATCAGTGATGCGAAACCACCGCAGGAAGGCTGATTCATCGTTCACCAATATTTCAACTATTTCTGATTTGGTTGCATGTAGACCATACTTCCAACTTGGAGGCATCGTTCACCGTTATATGGGACGCCAAACTCAAGTTATGGACGACGGCCAAGAAATTGGAGCATATTTGTTTCGTAGAACTGTCCCTTCTATCCATTTAACACCCGATGATGTTCGTTGGATGGTCGGAGCTTGGAGAGATAGAATCATTGTTTGCACAGGGACATATGGACCTACGCCACCACTGATTAAAGCATTTTTAGACTCTGGCGCTAAAGCTGTTATAAGTTCTTTAGCTCAGCCCCCGGAAACACAGCTGACAGCGCTCCACGGATCTGCAGAGTTCAGTGCTTTTGAAAACGGGAAGTTTGAGATTGGCGAGGAAGACGCTGAAGATGAAATCGAAGATGAGGAGGCTGAGCGTAGCAGTCCGCTGAGCGACTGGGAAGATAGTGAAAACGGGGACCCATCTACAGGTTTTTGGGATGACGATGAGGAAGAGGTGTCGCAATTTGTTTGTCAGTTATACGAGTCACTGTTTAGAGAGGGCGTGAGCGTGGATGCTGCTCTACGACAAGCTCTTGCCTCGCATCGGAAGTTGAGGTATTCATGCCATCTTCCCAGTATACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

436

Amino Acids

47.97

Weight (kDa)

5.27

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 393
AccI GTMKAC 2 cut(s) 712, 1300
AciI CCGC 2 cut(s) 652, 1106
AclWI GGATC 1 cut(s) 1015
AcoI YGGCCR 2 cut(s) 355, 781
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 2 cut(s) 593, 1086
AcyI GRCGYC 1 cut(s) 757
AfaI GTAC 2 cut(s) 117, 252
AfeI AGCGCT 1 cut(s) 999
AfiI CCNNNNNNNGG 8 cut(s) 98, 149, 338, 500, 658, 751, 1148, 1266
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 5 cut(s) 128, 244, 524, 687, 1031
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 1 cut(s) 1015
Aor51HI AGCGCT 1 cut(s) 999
AoxI GGCC 4 cut(s) 88, 341, 355, 781
ApeKI GCWGC 1 cut(s) 1238
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 3 cut(s) 394, 947, 1000
AspS9I GGNCC 7 cut(s) 27, 89, 341, 370, 437, 905, 1138
AsuC2I CCSGG 2 cut(s) 585, 980
AsuHPI GGTGA 4 cut(s) 276, 408, 667, 734
AvaII GGWCC 5 cut(s) 27, 370, 437, 905, 1138
BauI CACGAG 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 1065
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 1225
BccI CCATC 5 cut(s) 100, 316, 850, 1150, 1296
BceAI ACGGC 1 cut(s) 796
BcnI CCSGG 2 cut(s) 585, 980
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 3 cut(s) 98, 441, 1306
BfmI CTRYAG 3 cut(s) 325, 1011, 1146
BfoI RGCGCY 2 cut(s) 948, 1001
BglII AGATCT 1 cut(s) 41
BisI GCNGC 1 cut(s) 1239
BlpI GCTNAGC 3 cut(s) 972, 1092, 1109
BlsI GCNGC 1 cut(s) 1240
Bme1390I CCNGG 2 cut(s) 585, 980
Bme18I GGWCC 5 cut(s) 27, 370, 437, 905, 1138
BmgT120I GGNCC 7 cut(s) 27, 89, 341, 370, 437, 905, 1138
BmiI GGNNCC 3 cut(s) 372, 1139, 1140
BmrFI CCNGG 2 cut(s) 585, 980
BmrI ACTGGG 2 cut(s) 1126, 1290
BmsI GCATC 8 cut(s) 77, 290, 312, 379, 630, 744, 1225, 1273
BmuI ACTGGG 2 cut(s) 1126, 1290
BpiI GAAGAC 1 cut(s) 1065
Bpu1102I GCTNAGC 3 cut(s) 972, 1092, 1109
BpuEI CTTGAG 2 cut(s) 200, 750
BpuMI CCSGG 2 cut(s) 585, 980
Bsa29I ATCGAT 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 757
BsaJI CCNNGG 2 cut(s) 978, 1003
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 8 cut(s) 98, 149, 338, 500, 658, 751, 1148, 1266
Bse1I ACTGG 3 cut(s) 463, 1121, 1296
Bse3DI GCAATG 1 cut(s) 467
BseCI ATCGAT 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 978, 1003
BseGI GGATG 4 cut(s) 303, 861, 1165, 1240
BseLI CCNNNNNNNGG 8 cut(s) 98, 149, 338, 500, 658, 751, 1148, 1266
BseMI GCAATG 1 cut(s) 467
BseMII CTCAG 6 cut(s) 173, 414, 614, 986, 1083, 1100
BseNI ACTGG 3 cut(s) 463, 1121, 1296
BseRI GAGGAG 1 cut(s) 1100
BseXI GCAGC 1 cut(s) 1225
BshFI GGCC 4 cut(s) 90, 343, 357, 783
BshVI ATCGAT 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 196
BsiSI CCGG 2 cut(s) 585, 980
BslFI GGGAC 6 cut(s) 38, 356, 768, 803, 910, 1151
BslI CCNNNNNNNGG 8 cut(s) 98, 149, 338, 500, 658, 751, 1148, 1266
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 6 cut(s) 38, 356, 768, 803, 910, 1151
BsnI GGCC 4 cut(s) 90, 343, 357, 783
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 41, 1007
Bsp1720I GCTNAGC 3 cut(s) 972, 1092, 1109
BspACI CCGC 2 cut(s) 652, 1106
BspANI GGCC 4 cut(s) 90, 343, 357, 783
BspCNI CTCAG 6 cut(s) 172, 415, 615, 985, 1084, 1101
BspDI ATCGAT 1 cut(s) 363
BspLI GGNNCC 3 cut(s) 372, 1139, 1140
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 1 cut(s) 1015
BsrDI GCAATG 1 cut(s) 467
BsrI ACTGG 3 cut(s) 463, 1121, 1296
BssECI CCNNGG 2 cut(s) 978, 1003
BssMI GATC 2 cut(s) 41, 1007
BssNAI GTATAC 1 cut(s) 1301
BssNI GRCGYC 1 cut(s) 757
BssSI CACGAG 1 cut(s) 473
Bst1107I GTATAC 1 cut(s) 1301
Bst2BI CACGAG 1 cut(s) 473
Bst4CI ACNGT 5 cut(s) 250, 329, 746, 817, 1212
Bst6I CTCTTC 1 cut(s) 1167
BstACI GRCGYC 1 cut(s) 757
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 8 cut(s) 57, 159, 385, 423, 623, 972, 1092, 1109
BstDSI CCRYGG 1 cut(s) 1003
BstF5I GGATG 4 cut(s) 303, 861, 1165, 1240
BstH2I RGCGCY 2 cut(s) 948, 1001
BstHHI GCGC 3 cut(s) 394, 947, 1000
BstKTI GATC 2 cut(s) 44, 1010
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 2 cut(s) 41, 1007
BstMWI GCNNNNNNNGC 1 cut(s) 997
BstNSI RCATGY 2 cut(s) 53, 711
BstSCI CCNGG 2 cut(s) 583, 978
BstSFI CTRYAG 3 cut(s) 325, 1011, 1146
BstV1I GCAGC 1 cut(s) 1225
BstV2I GAAGAC 1 cut(s) 1065
BstX2I RGATCY 2 cut(s) 41, 1007
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 2 cut(s) 41, 1007
BstZ17I GTATAC 1 cut(s) 1301
Bsu15I ATCGAT 1 cut(s) 363
BsuRI GGCC 4 cut(s) 90, 343, 357, 783
BsuTUI ATCGAT 1 cut(s) 363
BtgI CCRYGG 1 cut(s) 1003
BtsCI GGATG 4 cut(s) 303, 861, 1165, 1240
BtsIMutI CAGTG 4 cut(s) 642, 917, 1027, 1208
Cac8I GCNNGC 1 cut(s) 594
CfoI GCGC 3 cut(s) 394, 947, 1000
Cfr13I GGNCC 7 cut(s) 27, 89, 341, 370, 437, 905, 1138
ClaI ATCGAT 1 cut(s) 363
CseI GACGC 2 cut(s) 765, 1070
Csp6I GTAC 2 cut(s) 116, 251
CviAII CATG 6 cut(s) 14, 50, 167, 173, 708, 1284
CviQI GTAC 2 cut(s) 116, 251
DdeI CTNAG 8 cut(s) 57, 159, 385, 423, 623, 972, 1092, 1109
DpnI GATC 2 cut(s) 43, 1009
DpnII GATC 2 cut(s) 41, 1007
EaeI YGGCCR 2 cut(s) 355, 781
Eam1104I CTCTTC 1 cut(s) 1167
EarI CTCTTC 1 cut(s) 1167
Eco32I GATATC 1 cut(s) 633
Eco47I GGWCC 5 cut(s) 27, 370, 437, 905, 1138
Eco47III AGCGCT 1 cut(s) 999
Eco57I CTGAAG 2 cut(s) 593, 1086
EcoO109I RGGNCCY 2 cut(s) 437, 1138
EcoRV GATATC 1 cut(s) 633
FaeI CATG 6 cut(s) 17, 53, 170, 176, 711, 1287
FaqI GGGAC 6 cut(s) 38, 356, 768, 803, 910, 1151
FatI CATG 6 cut(s) 13, 49, 166, 172, 707, 1283
FauNDI CATATG 1 cut(s) 901
FblI GTMKAC 2 cut(s) 712, 1300
Fnu4HI GCNGC 1 cut(s) 1239
FokI GGATG 4 cut(s) 290, 868, 1172, 1247
Fsp4HI GCNGC 1 cut(s) 1239
FspBI CTAG 3 cut(s) 98, 441, 1306
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 3 cut(s) 393, 946, 999
GluI GCNGC 1 cut(s) 1239
HaeII RGCGCY 2 cut(s) 948, 1001
HaeIII GGCC 4 cut(s) 90, 343, 357, 783
HapII CCGG 2 cut(s) 585, 980
HgaI GACGC 2 cut(s) 765, 1070
HhaI GCGC 3 cut(s) 394, 947, 1000
Hin1I GRCGYC 1 cut(s) 757
Hin1II CATG 6 cut(s) 17, 53, 170, 176, 711, 1287
Hin6I GCGC 3 cut(s) 392, 945, 998
HinP1I GCGC 3 cut(s) 392, 945, 998
HindIII AAGCTT 2 cut(s) 129, 382
HinfI GANTC 8 cut(s) 182, 360, 425, 530, 665, 879, 938, 1205
HpaII CCGG 2 cut(s) 585, 980
HphI GGTGA 4 cut(s) 276, 408, 667, 734
Hpy166II GTNNAC 5 cut(s) 317, 675, 713, 742, 1301
Hpy188I TCNGA 6 cut(s) 31, 162, 612, 697, 863, 1269
Hpy188III TCNNGA 3 cut(s) 234, 332, 399
Hpy8I GTNNAC 5 cut(s) 317, 675, 713, 742, 1301
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 3 cut(s) 530, 652, 831
HpyCH4III ACNGT 5 cut(s) 250, 329, 746, 817, 1212
HpyCH4V TGCA 9 cut(s) 176, 223, 281, 472, 511, 596, 707, 891, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 997
HpyF3I CTNAG 8 cut(s) 57, 159, 385, 423, 623, 972, 1092, 1109
Hsp92I GRCGYC 1 cut(s) 757
Hsp92II CATG 6 cut(s) 17, 53, 170, 176, 711, 1287
HspAI GCGC 3 cut(s) 392, 945, 998
KflI GGGWCCC 1 cut(s) 1138
Kzo9I GATC 2 cut(s) 41, 1007
LmnI GCTCC 4 cut(s) 407, 794, 863, 1005
Lsp1109I GCAGC 1 cut(s) 1225
LweI GCATC 8 cut(s) 77, 290, 312, 379, 630, 744, 1225, 1273
MaeI CTAG 3 cut(s) 98, 441, 1306
MaeIII GTNAC 4 cut(s) 74, 414, 480, 1206
MalI GATC 2 cut(s) 43, 1009
MboI GATC 2 cut(s) 41, 1007
MboII GAAGA 7 cut(s) 599, 1070, 1079, 1091, 1133, 1184, 1283
MflI RGATCY 2 cut(s) 41, 1007
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 5 cut(s) 34, 145, 601, 789, 1184
MlyI GAGTC 5 cut(s) 191, 434, 524, 932, 1214
MmeI TCCRAC 5 cut(s) 34, 83, 748, 833, 841
MseI TTAA 5 cut(s) 38, 380, 548, 833, 924
MslI CAYNNNNRTG 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 991, 1108
MspI CCGG 2 cut(s) 585, 980
MspR9I CCNGG 2 cut(s) 585, 980
MwoI GCNNNNNNNGC 1 cut(s) 997
NciI CCSGG 2 cut(s) 585, 980
NdeI CATATG 1 cut(s) 901
NdeII GATC 2 cut(s) 41, 1007
NlaIII CATG 6 cut(s) 17, 53, 170, 176, 711, 1287
NlaIV GGNNCC 3 cut(s) 372, 1139, 1140
NmuCI GTSAC 3 cut(s) 74, 414, 1206
NsbI TGCGCA 1 cut(s) 393
NspI RCATGY 2 cut(s) 53, 711
PciI ACATGT 1 cut(s) 49
PfeI GAWTC 3 cut(s) 360, 665, 879
PkrI GCNGC 1 cut(s) 1240
PleI GAGTC 5 cut(s) 190, 433, 524, 932, 1213
PpsI GAGTC 5 cut(s) 190, 433, 524, 932, 1213
PpuMI RGGWCCY 2 cut(s) 437, 1138
PscI ACATGT 1 cut(s) 49
PsiI TTATAA 1 cut(s) 959
Psp5II RGGWCCY 2 cut(s) 437, 1138
PspN4I GGNNCC 3 cut(s) 372, 1139, 1140
PspPI GGNCC 7 cut(s) 27, 89, 341, 370, 437, 905, 1138
PspPPI RGGWCCY 2 cut(s) 437, 1138
PstI CTGCAG 1 cut(s) 1015
PsuI RGATCY 2 cut(s) 41, 1007
PvuII CAGCTG 1 cut(s) 991
RsaI GTAC 2 cut(s) 117, 252
RsaNI GTAC 2 cut(s) 116, 251
RseI CAYNNNNRTG 1 cut(s) 171
SaqAI TTAA 5 cut(s) 38, 380, 548, 833, 924
SatI GCNGC 1 cut(s) 1239
Sau3AI GATC 2 cut(s) 41, 1007
Sau96I GGNCC 7 cut(s) 27, 89, 341, 370, 437, 905, 1138
SchI GAGTC 5 cut(s) 191, 434, 524, 932, 1214
ScrFI CCNGG 2 cut(s) 585, 980
SduI GDGCHC 1 cut(s) 196
SfaNI GCATC 8 cut(s) 77, 290, 312, 379, 630, 744, 1225, 1273
SfcI CTRYAG 3 cut(s) 325, 1011, 1146
SinI GGWCC 5 cut(s) 27, 370, 437, 905, 1138
SmiMI CAYNNNNRTG 1 cut(s) 171
SmlI CTYRAG 2 cut(s) 179, 765
SmoI CTYRAG 2 cut(s) 179, 765
Sse9I AATT 5 cut(s) 34, 145, 601, 789, 1184
SsiI CCGC 2 cut(s) 652, 1106
SspI AATATT 2 cut(s) 546, 682
SspMI CTAG 3 cut(s) 98, 441, 1306
StyD4I CCNGG 2 cut(s) 583, 978
TaaI ACNGT 5 cut(s) 250, 329, 746, 817, 1212
TaqI TCGA 2 cut(s) 363, 1077
TasI AATT 5 cut(s) 34, 145, 601, 789, 1184
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 3 cut(s) 360, 665, 879
Tru1I TTAA 5 cut(s) 38, 380, 548, 833, 924
Tru9I TTAA 5 cut(s) 38, 380, 548, 833, 924
TscAI CASTG 4 cut(s) 642, 924, 1027, 1215
TseFI GTSAC 3 cut(s) 74, 414, 1206
TseI GCWGC 1 cut(s) 1238
Tsp45I GTSAC 3 cut(s) 74, 414, 1206
TspDTI ATGAA 3 cut(s) 657, 1086, 1272
TspGWI ACGGA 1 cut(s) 1020
TspRI CASTG 4 cut(s) 642, 924, 1027, 1215
VpaK11BI GGWCC 5 cut(s) 27, 370, 437, 905, 1138
XapI RAATTY 1 cut(s) 34
XceI RCATGY 2 cut(s) 53, 711
XcmI CCANNNNNNNNNTGG 1 cut(s) 99
XmiI GTMKAC 2 cut(s) 712, 1300
XspI CTAG 3 cut(s) 98, 441, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.