Rmu_sc0000500.1_g000003

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000500.1
OG0004422
Physical Location & Seq
Reverse (-)
3759 .. 11159
7401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000500.1_g000003.1.cds

Sequence Viewer

Length: 4164 bp
atgtcctggggactgggatggaagcgcccctcggagatcttccatctcacgctctcctacggcaccgaggacccgccgacgccggagaattccagcggcggcacgcggtcctcggcgtcgtcgttggagtcttcctcgtcggtagtgtcgcagcaggatcaagaattggggttccggatcgatctggattggtccgccggcgacgacgaggaccaggtggcgctgcggctccagtcgcagctgatggtggcgctgccgatgccgcaggacacggtggtcgtggagctgaggccggaggaggcgaatgtgagtgtggatatgagggttgtgcggcggagagagccgttgagggcggtgagtatgaccaagacgggcgggtcgggtcagcagaatgacggcaccggagtgttgacccggttgttgaggtccaatttctcttcttccatgccagctgtcgccgaaggcgcggcggcttgcggtgtgcattggcagtgcgtcacggtggtccgtctctgtggttgtggtttgtcggtattgccggtagagctaaccagactgcctcttcttgagaagctacaccttgataacaataaactggaacgtttgccttctgaactcggggaattgagaagcttaaaggtgctcagggttgactacaacatgctggtctcagtaccggtggaactgagacagtgtgttggattagtggaactttctttggaacacaacaagcttgttcggcctctgcttgatttcagggctatggctgagctccgggtacttaggctatttggaaaccctcttgaatttcttcctgaaatcttacctctacacaaacttcatcatttatctctcgcaaatatcaggattgtggcagatgacagcttgagatcagtgaatgtgcagatagagatggaaaacagttcgtattttggtgcatctaggcataagctaagcgcctttttcgcccttatattccgtttttcttcttgtcatcaccctttactagcatctgcactagcaaagataatgcaagacgaaggaaaccgtgcagtcgttggtaaagatgagaatgcagtgcgacagcttattagtatgatcagtagtgatgaccgtcatgtggttcaagaagcatgttctgctctttcatctcttgctgcagacgtttcagttgcaatgcagttgatgaaagccgacattatgcaacccattgagacggtactgagatctgttctccaagaggaattgatttctgtattacaagttgtggtgaagctggcttttgcatctgatgccgtagctcagaaaatgttgaccaaggatgtattgaagtcgttaaaactcttatgtgcccacagaaccccagaggtgcaaaggttagctttggtagcagttggaaatttggcgttctgtttggagaatcgtcgccttctggttacttctgaaagcttgtgtgaacttctcatgcgcttaacggttgtgcctgaaccatgtgtgaataaagctgcagctcgtgctttggcaattcttggggaaaatggaaatctacgacgtgccataagggggagacaggtgccaaagcaaggattacgcatactctcaatggatggtggtggtatgaaaggtctggcaactgtgcaaattcttaaagcaattgagaagggaactggaaaacccatacatgagttgtttgacctcatatgcggcacatcaactggtggcatgcttgctgttgctcttggcattaagctaatgtcattagatcagtgtgaagaaatatacaaaaaccttggaaaacttgtctttgctgaacctgctccaaaggacaatgaagctgcaagttggagagaaaagttggatcagctttataaaagttcgtcgcagagtttcagagttgttgtacatggatctaaacatagtgcagatcagtttgagacattgttgaaggaaatgtgtgctgatgaggatggagatctgatgatagagtcggcagtgaaaaacattcccaaagtttttgtagtgtcaactttggtgagcatgacgccagctcagccgttcttattccggaattatcagtaccctgcgggaacgccagaagtagctcttgtagtttcagagagttcaggaatcactgtgcaagaaccaactactttgggtactgaccttggctataaacgcagtgcttttatgggaagttgtaagcatcaaatatggcaagctataagagcatcatctgctgcgccatactatcttgatgatttctcagatgatattcatcgttggcaagatggtgcaatagtggcaaacaatcctacaatctttgccatcagagaagcacaacttttatggcctgacacgaaaattgattgcttagtttctgttggctgtggttctgttccgacaaaggtgcgaaaaggtggttggcgttatctggatactgggcaagtattgatagagagttcttgctctgtggaacgcgtggaggaagccttgagcacattgctacccatgctccctggaatacaatactttcggtttaatccagttgatgaacgttgtgatatggaactggatgagactgatccagctgtctggctgaaattggaagcctcagttggggaatatattcagaaaaattctcttactcttaaggatgcttgtgagagactactgctgccattccaaaacgacgaaaagtggtctgagaatttgagatcccaacatttccccaaggcaaatgaggttgatgaacgttgtgatatggaactggatgagactgatccagctgtctggctgaaattggaagcctcagttggggaatatattcagaaaaattctcttactcttaaggatgcttgtgagagactactgctgccattccaaaacgacgaaaagtggtctgagaatttgagatcccaacatttccccaaggcaaatgaggtagatgtgaaaagcccatctctaggttggaggcgtaatgtgcttcttgttgaagcttctcatagccccaattctggtcgagcttcgaatcacgctcatgaacttgaatcattttgtgctcgtaatggaatacgggtatctttaatgcaaggaatatcagggtttgtgaaaaccgcagcagcaacaacattcccaacaccatttcagtcgcctttgtttcctgcaagcgtcccatcaagcccacttttctacagtcctgactttggtccgcagagggttggccgaattgatatggtcccacctctgagcttagatggccaacctggaaagggagctgcctcaccaccaaagtctccttcaggacctaggcagctttctgtacccgttcagtcattgcatgagaagttacaaaattcgccacaagtgggcattgtgcatttggcccttcaaaatgactcaattggctcgattttaagttggcagaatgatgtatttgtggttgctgaacctggagaacttgcagataactttctgcagagtgttaagttgagcttgttatcagttatgcgaaaccatcgcaagaaggctggatcacctctttccaatatttcaactgtttctgatttggttgcttgtaaaccaatcttccaacttggatgtatcgttcaccgttatatgggacgccaaactcaagtaatggaagatggccaagaaattggggcgtatttgtttcgtagaactgtcccctctattcacttgacacctgatgatgttcgatggatggttggagcttggagagacaggatcattatttgtacagggacaaatgggcctacgccaactatgatcaaagcatttttagactctggtgccaaagcggttatatcttccacagtacagccccaagaaacacagttaacattcgggtctactgatttcagtgttcttgaaaagtttgagattggtgatgaggaggctgaagatgaagaagttgaagatgaggtggctgaacctgaaagtccggtgagtgattgggaagacagtgaaaatggagaccgctctatcggctttggggatgatgatgaggaagaagtgtctcaattcgtctgtcatttgtatgactcactgtttcgagagggtacgagtgtggatgttgctttacgacatgctcttgcttcacatcggaagttgaggtattcatgtcatcttcccagtatacagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1387

Amino Acids

153.11

Weight (kDa)

5.42

Isoelectric Point (pI)

50.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1869
AasI GACNNNNNNGTC 2 cut(s) 275, 376
Acc36I ACCTGC 1 cut(s) 1822
AccB1I GGYRCC 4 cut(s) 62, 398, 1573, 3809
AccBSI CCGCTC 1 cut(s) 3999
AccI GTMKAC 2 cut(s) 3869, 4156
AccII CGCG 3 cut(s) 106, 467, 2478
AccIII TCCGGA 2 cut(s) 174, 2064
AclI AACGTT 3 cut(s) 601, 2554, 2752
AcoI YGGCCR 3 cut(s) 3220, 3256, 3646
AcsI RAATTY 9 cut(s) 88, 806, 1399, 1641, 2635, 2707, 2833, 2905, 3352
AcuI CTGAAG 2 cut(s) 3282, 3939
AcyI GRCGYC 4 cut(s) 80, 116, 2042, 3622
AflII CTTAAG 2 cut(s) 2648, 2846
AflIII ACRYGT 1 cut(s) 2476
AgeI ACCGGT 1 cut(s) 676
AjiI CACGTC 1 cut(s) 1553
AjnI CCWGG 5 cut(s) 5, 213, 2515, 3262, 3448
AjuI GAANNNNNNNTTGG 8 cut(s) 2404, 2436, 2598, 2630, 2796, 2828, 3280, 3312
AleI CACNNNNGTG 1 cut(s) 404
AloI GAACNNNNNNTCC 2 cut(s) 3588, 3620
Alw21I GWGCWC 4 cut(s) 645, 774, 2498, 3061
Ama87I CYCGRG 1 cut(s) 617
Aor13HI TCCGGA 2 cut(s) 174, 2064
AoxI GGCC 8 cut(s) 290, 740, 2348, 3220, 3256, 3381, 3646, 3770
ApoI RAATTY 9 cut(s) 88, 806, 1399, 1641, 2635, 2707, 2833, 2905, 3352
AsiGI ACCGGT 1 cut(s) 676
Asp700I GAANNNNTTC 3 cut(s) 810, 2625, 2823
AspA2I CCTAGG 1 cut(s) 3305
AspLEI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
AsuC2I CCSGG 2 cut(s) 415, 776
AsuHPI GGTGA 9 cut(s) 367, 989, 1282, 2044, 3273, 3525, 3599, 3917, 3976
AsuII TTCGAA 1 cut(s) 3026
AvaI CYCGRG 1 cut(s) 617
AvaII GGWCC 9 cut(s) 70, 108, 192, 211, 426, 505, 3206, 3235, 3302
AvrII CCTAGG 1 cut(s) 3305
BaeGI GKGCMC 1 cut(s) 1354
BaeI ACNNNNGTAYC 2 cut(s) 4071, 4104
BalI TGGCCA 2 cut(s) 3258, 3648
BanI GGYRCC 4 cut(s) 62, 398, 1573, 3809
BanII GRGCYC 1 cut(s) 774
BauI CACGAG 1 cut(s) 1512
BbsI GAAGAC 2 cut(s) 123, 3984
Bbv12I GWGCWC 4 cut(s) 645, 774, 2498, 3061
BbvCI CCTCAGC 1 cut(s) 287
BceAI ACGGC 5 cut(s) 76, 328, 412, 1280, 2038
BcgI CGANNNNNNTGC 2 cut(s) 2389, 2423
BciT130I CCWGG 5 cut(s) 7, 215, 2517, 3264, 3450
BciVI GTATCC 1 cut(s) 2428
BclI TGATCA 2 cut(s) 1098, 3786
BcnI CCSGG 2 cut(s) 415, 776
BfaI CTAG 5 cut(s) 942, 1007, 1019, 2963, 3306
BfmI CTRYAG 4 cut(s) 1158, 1506, 3190, 3473
BfoI RGCGCY 4 cut(s) 28, 224, 254, 960
BfrI CTTAAG 2 cut(s) 2648, 2846
BfuAI ACCTGC 1 cut(s) 1822
BfuI GTATCC 1 cut(s) 2428
BglII AGATCT 3 cut(s) 36, 1226, 1972
BlnI CCTAGG 1 cut(s) 3305
BlpI GCTNAGC 3 cut(s) 768, 953, 2049
Bme1390I CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3264, 3450
Bme18I GGWCC 9 cut(s) 70, 108, 192, 211, 426, 505, 3206, 3235, 3302
BmeT110I CYCGRG 1 cut(s) 617
BmgBI CACGTC 1 cut(s) 1553
BmiI GGNNCC 8 cut(s) 64, 72, 173, 230, 400, 1575, 3237, 3811
BmrFI CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3264, 3450
BmrI ACTGGG 3 cut(s) 23, 2448, 4146
BmsI GCATC 9 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644, 2842
BmuI ACTGGG 3 cut(s) 23, 2448, 4146
BoxI GACNNNNGTC 1 cut(s) 3204
BpiI GAAGAC 2 cut(s) 123, 3984
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 2 cut(s) 215, 3471
Bpu10I CCTNAGC 2 cut(s) 287, 644
Bpu1102I GCTNAGC 3 cut(s) 768, 953, 2049
Bpu14I TTCGAA 1 cut(s) 3026
BpuEI CTTGAG 4 cut(s) 587, 907, 2512, 3615
BpuMI CCSGG 2 cut(s) 415, 776
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 2 cut(s) 1971, 2274
BsaHI GRCGYC 4 cut(s) 80, 116, 2042, 3622
BsaI GGTCTC 2 cut(s) 673, 3987
BsaWI WCCGGW 5 cut(s) 174, 401, 676, 2064, 3961
BsaXI ACNNNNNCTCC 2 cut(s) 2496, 2526
Bse118I RCCGGY 3 cut(s) 197, 538, 676
Bse3DI GCAATG 3 cut(s) 1182, 2498, 3332
Bse8I GATNNNNATC 2 cut(s) 1971, 2274
BseAI TCCGGA 2 cut(s) 174, 2064
BseBI CCWGG 5 cut(s) 7, 215, 2517, 3264, 3450
BseCI ATCGAT 1 cut(s) 180
BseJI GATNNNNATC 2 cut(s) 1971, 2274
BseMI GCAATG 3 cut(s) 1182, 2498, 3332
BseRI GAGGAG 2 cut(s) 311, 3926
BseSI GKGCMC 1 cut(s) 1354
BsgI GTGCAG 4 cut(s) 923, 999, 1071, 1941
Bsh1236I CGCG 3 cut(s) 106, 467, 2478
BshFI GGCC 8 cut(s) 292, 742, 2350, 3222, 3258, 3383, 3648, 3772
BshNI GGYRCC 4 cut(s) 62, 398, 1573, 3809
BshTI ACCGGT 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 4 cut(s) 645, 774, 2498, 3061
BsiHKCI CYCGRG 1 cut(s) 617
BslFI GGGAC 6 cut(s) 24, 3155, 3221, 3633, 3668, 3775
BsmBI CGTCTC 2 cut(s) 515, 1208
BsmFI GGGAC 6 cut(s) 24, 3155, 3221, 3633, 3668, 3775
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 8 cut(s) 292, 742, 2350, 3222, 3258, 3383, 3648, 3772
Bso31I GGTCTC 2 cut(s) 673, 3987
BsoBI CYCGRG 1 cut(s) 617
Bsp119I TTCGAA 1 cut(s) 3026
Bsp1286I GDGCHC 5 cut(s) 645, 774, 1354, 2498, 3061
Bsp13I TCCGGA 2 cut(s) 174, 2064
Bsp1407I TGTACA 2 cut(s) 1900, 3755
Bsp1720I GCTNAGC 3 cut(s) 768, 953, 2049
BspANI GGCC 8 cut(s) 292, 742, 2350, 3222, 3258, 3383, 3648, 3772
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 2 cut(s) 174, 2064
BspFNI CGCG 3 cut(s) 106, 467, 2478
BspHI TCATGA 1 cut(s) 3037
BspLI GGNNCC 8 cut(s) 64, 72, 173, 230, 400, 1575, 3237, 3811
BspMAI CTGCAG 3 cut(s) 1162, 1510, 3477
BspMI ACCTGC 1 cut(s) 1822
BspT104I TTCGAA 1 cut(s) 3026
BspT107I GGYRCC 4 cut(s) 62, 398, 1573, 3809
BspTI CTTAAG 2 cut(s) 2648, 2846
BspTNI GGTCTC 2 cut(s) 673, 3987
BsrBI CCGCTC 1 cut(s) 3999
BsrDI GCAATG 3 cut(s) 1182, 2498, 3332
BsrFI RCCGGY 3 cut(s) 197, 538, 676
BsrGI TGTACA 2 cut(s) 1900, 3755
BssAI RCCGGY 3 cut(s) 197, 538, 676
BssNAI GTATAC 1 cut(s) 4157
BssNI GRCGYC 4 cut(s) 80, 116, 2042, 3622
BssSI CACGAG 1 cut(s) 1512
BssT1I CCWWGG 6 cut(s) 1317, 1789, 2164, 2730, 2928, 3305
Bst1107I GTATAC 1 cut(s) 4157
Bst2BI CACGAG 1 cut(s) 1512
Bst2UI CCWGG 5 cut(s) 7, 215, 2517, 3264, 3450
Bst6I CTCTTC 2 cut(s) 442, 567
BstACI GRCGYC 4 cut(s) 80, 116, 2042, 3622
BstAFI CTTAAG 2 cut(s) 2648, 2846
BstAPI GCANNNNNTGC 4 cut(s) 1139, 1292, 1514, 2234
BstAUI TGTACA 2 cut(s) 1900, 3755
BstBI TTCGAA 1 cut(s) 3026
BstFNI CGCG 3 cut(s) 106, 467, 2478
BstH2I RGCGCY 4 cut(s) 28, 224, 254, 960
BstHHI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
BstNI CCWGG 5 cut(s) 7, 215, 2517, 3264, 3450
BstNSI RCATGY 4 cut(s) 664, 1137, 1726, 4109
BstPAI GACNNNNGTC 1 cut(s) 3204
BstSCI CCNGG 7 cut(s) 5, 213, 413, 774, 2515, 3262, 3448
BstSFI CTRYAG 4 cut(s) 1158, 1506, 3190, 3473
BstSLI GKGCMC 1 cut(s) 1354
BstUI CGCG 3 cut(s) 106, 467, 2478
BstV2I GAAGAC 2 cut(s) 123, 3984
BstX2I RGATCY 6 cut(s) 36, 1226, 1907, 1972, 2714, 2912
BstXI CCANNNNNNTGG 3 cut(s) 2592, 2790, 3656
BstYI RGATCY 6 cut(s) 36, 1226, 1907, 1972, 2714, 2912
BstZ17I GTATAC 1 cut(s) 4157
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 1 cut(s) 2428
BsuRI GGCC 8 cut(s) 292, 742, 2350, 3222, 3258, 3383, 3648, 3772
BsuTUI ATCGAT 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 3500
BtrI CACGTC 1 cut(s) 1553
BtsI GCAGTG 4 cut(s) 497, 1083, 1998, 2185
BveI ACCTGC 1 cut(s) 1822
CciI TCATGA 1 cut(s) 3037
CfoI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
Cfr10I RCCGGY 3 cut(s) 197, 538, 676
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 6 cut(s) 88, 105, 484, 2050, 3157, 3630
CsiI ACCWGGT 1 cut(s) 213
CspAI ACCGGT 1 cut(s) 676
DrdI GACNNNNNNGTC 2 cut(s) 275, 376
DseDI GACNNNNNNGTC 2 cut(s) 275, 376
EaeI YGGCCR 3 cut(s) 3220, 3256, 3646
Eam1104I CTCTTC 2 cut(s) 442, 567
EarI CTCTTC 2 cut(s) 442, 567
EciI GGCGGA 2 cut(s) 184, 349
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 6 cut(s) 1317, 1789, 2164, 2730, 2928, 3305
Eco24I GRGCYC 1 cut(s) 774
Eco31I GGTCTC 2 cut(s) 673, 3987
Eco47I GGWCC 9 cut(s) 70, 108, 192, 211, 426, 505, 3206, 3235, 3302
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 2 cut(s) 3282, 3939
Eco88I CYCGRG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 2 cut(s) 70, 3302
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 5 cut(s) 5, 213, 2515, 3262, 3448
EcoT14I CCWWGG 6 cut(s) 1317, 1789, 2164, 2730, 2928, 3305
EcoT38I GRGCYC 1 cut(s) 774
ErhI CCWWGG 6 cut(s) 1317, 1789, 2164, 2730, 2928, 3305
Esp3I CGTCTC 2 cut(s) 515, 1208
FalI AAGNNNNNCTT 8 cut(s) 1366, 1398, 2088, 2120, 2325, 2357, 3476, 3508
FaqI GGGAC 6 cut(s) 24, 3155, 3221, 3633, 3668, 3775
FauI CCCGC 3 cut(s) 81, 368, 2077
FauNDI CATATG 1 cut(s) 1700
FbaI TGATCA 2 cut(s) 1098, 3786
FblI GTMKAC 2 cut(s) 3869, 4156
FriOI GRGCYC 1 cut(s) 774
FspBI CTAG 5 cut(s) 942, 1007, 1019, 2963, 3306
GlaI GCGC 7 cut(s) 26, 222, 252, 466, 958, 1469, 2241
GsuI CTGGAG 2 cut(s) 215, 3471
HaeII RGCGCY 4 cut(s) 28, 224, 254, 960
HaeIII GGCC 8 cut(s) 292, 742, 2350, 3222, 3258, 3383, 3648, 3772
HgaI GACGC 6 cut(s) 88, 105, 484, 2050, 3157, 3630
HhaI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
Hin1I GRCGYC 4 cut(s) 80, 116, 2042, 3622
Hin6I GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
HinP1I GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
HincII GTYRAC 5 cut(s) 411, 652, 1314, 2025, 3858
HindII GTYRAC 5 cut(s) 411, 652, 1314, 2025, 3858
HindIII AAGCTT 4 cut(s) 631, 731, 1447, 2994
HinfI GANTC 9 cut(s) 128, 1420, 1985, 2127, 3028, 3047, 3395, 3803, 4061
HpaI GTTAAC 1 cut(s) 3858
HphI GGTGA 9 cut(s) 367, 989, 1282, 2044, 3273, 3525, 3599, 3917, 3976
HpyAV CCTTC 9 cut(s) 455, 618, 1034, 1439, 1654, 1939, 3306, 3395, 3517
HpyCH4IV ACGT 5 cut(s) 601, 1164, 1552, 2554, 2752
HpySE526I ACGT 5 cut(s) 601, 1164, 1552, 2554, 2752
Hsp92I GRCGYC 4 cut(s) 80, 116, 2042, 3622
HspAI GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
Kpn2I TCCGGA 2 cut(s) 174, 2064
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 1098, 3786
KspAI GTTAAC 1 cut(s) 3858
LmnI GCTCC 7 cut(s) 234, 283, 777, 1822, 2517, 3272, 3728
LweI GCATC 9 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644, 2842
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 5 cut(s) 942, 1007, 1019, 2963, 3306
MaeII ACGT 5 cut(s) 601, 1164, 1552, 2554, 2752
MaeIII GTNAC 3 cut(s) 496, 1435, 3345
MbiI CCGCTC 1 cut(s) 3999
MfeI CAATTG 2 cut(s) 1653, 3399
MflI RGATCY 6 cut(s) 36, 1226, 1907, 1972, 2714, 2912
MhlI GDGCHC 5 cut(s) 645, 774, 1354, 2498, 3061
MlsI TGGCCA 2 cut(s) 3258, 3648
MluI ACGCGT 1 cut(s) 2476
MluNI TGGCCA 2 cut(s) 3258, 3648
MlyI GAGTC 5 cut(s) 137, 1994, 3389, 3797, 4055
MmeI TCCRAC 9 cut(s) 105, 679, 1375, 1823, 1836, 2423, 2948, 3613, 3706
Mox20I TGGCCA 2 cut(s) 3258, 3648
MreI CGCCGGCG 1 cut(s) 197
MroI TCCGGA 2 cut(s) 174, 2064
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 3 cut(s) 810, 2625, 2823
MscI TGGCCA 2 cut(s) 3258, 3648
MslI CAYNNNNRTG 3 cut(s) 404, 3036, 4056
Msp20I TGGCCA 2 cut(s) 3258, 3648
MspA1I CMGCKG 5 cut(s) 96, 241, 452, 2588, 2786
MspCI CTTAAG 2 cut(s) 2648, 2846
MspR9I CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3264, 3450
MunI CAATTG 2 cut(s) 1653, 3399
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 5 cut(s) 7, 215, 2517, 3264, 3450
MvnI CGCG 3 cut(s) 106, 467, 2478
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 415, 776
NdeI CATATG 1 cut(s) 1700
NgoMIV GCCGGC 1 cut(s) 197
NlaIV GGNNCC 8 cut(s) 64, 72, 173, 230, 400, 1575, 3237, 3811
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 496
NspI RCATGY 4 cut(s) 664, 1137, 1726, 4109
NspV TTCGAA 1 cut(s) 3026
OliI CACNNNNGTG 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1726
PagI TCATGA 1 cut(s) 3037
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 1078
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 3 cut(s) 810, 2625, 2823
PfeI GAWTC 4 cut(s) 1420, 2127, 3028, 3047
PinAI ACCGGT 1 cut(s) 676
PleI GAGTC 5 cut(s) 136, 1993, 3389, 3797, 4055
PpsI GAGTC 5 cut(s) 136, 1993, 3389, 3797, 4055
PpuMI RGGWCCY 2 cut(s) 70, 3302
PshAI GACNNNNGTC 1 cut(s) 3204
PsiI TTATAA 1 cut(s) 1869
Psp124BI GAGCTC 1 cut(s) 774
Psp1406I AACGTT 3 cut(s) 601, 2554, 2752
Psp5II RGGWCCY 2 cut(s) 70, 3302
Psp6I CCWGG 5 cut(s) 5, 213, 2515, 3262, 3448
PspGI CCWGG 5 cut(s) 5, 213, 2515, 3262, 3448
PspN4I GGNNCC 8 cut(s) 64, 72, 173, 230, 400, 1575, 3237, 3811
PspPPI RGGWCCY 2 cut(s) 70, 3302
PstI CTGCAG 3 cut(s) 1162, 1510, 3477
PsuI RGATCY 6 cut(s) 36, 1226, 1907, 1972, 2714, 2912
PvuII CAGCTG 4 cut(s) 241, 452, 2588, 2786
RseI CAYNNNNRTG 3 cut(s) 404, 3036, 4056
SacI GAGCTC 1 cut(s) 774
SchI GAGTC 5 cut(s) 137, 1994, 3389, 3797, 4055
ScrFI CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3264, 3450
SduI GDGCHC 5 cut(s) 645, 774, 1354, 2498, 3061
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 9 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644, 2842
SfcI CTRYAG 4 cut(s) 1158, 1506, 3190, 3473
SfuI TTCGAA 1 cut(s) 3026
SgrAI CRCCGGYG 1 cut(s) 197
SinI GGWCC 9 cut(s) 70, 108, 192, 211, 426, 505, 3206, 3235, 3302
SmiMI CAYNNNNRTG 3 cut(s) 404, 3036, 4056
SmlI CTYRAG 6 cut(s) 566, 886, 2491, 2648, 2846, 3630
SmoI CTYRAG 6 cut(s) 566, 886, 2491, 2648, 2846, 3630
SphI GCATGC 1 cut(s) 1726
SspI AATATT 1 cut(s) 3547
SspMI CTAG 5 cut(s) 942, 1007, 1019, 2963, 3306
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 7 cut(s) 5, 213, 413, 774, 2515, 3262, 3448
StyI CCWWGG 6 cut(s) 1317, 1789, 2164, 2730, 2928, 3305
TaiI ACGT 5 cut(s) 604, 1167, 1555, 2557, 2755
TaqI TCGA 6 cut(s) 180, 3019, 3026, 3407, 3715, 4072
TatI WGTACW 3 cut(s) 1900, 3755, 3835
TauI GCSGC 8 cut(s) 99, 102, 229, 265, 334, 470, 473, 1707
TfiI GAWTC 4 cut(s) 1420, 2127, 3028, 3047
TseFI GTSAC 1 cut(s) 496
Tsp45I GTSAC 1 cut(s) 496
TspGWI ACGGA 2 cut(s) 497, 968
Vha464I CTTAAG 2 cut(s) 2648, 2846
VpaK11BI GGWCC 9 cut(s) 70, 108, 192, 211, 426, 505, 3206, 3235, 3302
XapI RAATTY 9 cut(s) 88, 806, 1399, 1641, 2635, 2707, 2833, 2905, 3352
XceI RCATGY 4 cut(s) 664, 1137, 1726, 4109
XcmI CCANNNNNNNNNTGG 1 cut(s) 2964
XmaJI CCTAGG 1 cut(s) 3305
XmiI GTMKAC 2 cut(s) 3869, 4156
XmnI GAANNNNTTC 3 cut(s) 810, 2625, 2823
XspI CTAG 5 cut(s) 942, 1007, 1019, 2963, 3306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.