Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004422

Physical Location & Seq

Genomic Coordinates Forward (+)

46322894 .. 46329645

Gene Structure 6752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1342900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3945 bp

ATGTCTTGGGGACTGGGCTGGAAGCGGCCCTTGGAGATCTTCCATCTCGTGCTCACGTACGGCACCGAGGGTCCGCCGGAGACCTTCGACCGTACGTCGTCGGCGTCGTCCTCGTCGTCCTCGTCTTCCGTGGCGACGCAGGATCAAGAATCAGGGTATCGGATCGACTTGGATTGGCAGGCTGGGGACGATGAGGAGCAGGTGGCTCTGAGGCTCCAGTCGCAGCTAATGGTGGCGTTGCCGATGCCTCAGGACACGGTGGTGGTCGAATTGAGGACCGAGGAGGCAGAGGAAGCGAATGTGGGTGTGGACATGAGGGTGGTGAGGCGCAGAGAGCCGTTGAGAGCTGTGACCATGACCAAGACGGCCGGGTCCGGCCAGCAAAGCGATGGCACTGGAGTCTTGACTCGCTTGCTGCGCTCCAATTTCGCTTCAACAATGCCTGCAGTTGCCGACGGCGAGGCGGCCTGCGGTGTGCATTGGCAGTGCGTCACCGTGGTCAATCTGAGTGGTTGTGGGTTGTCGGTTTTACCGGTAGAGCTAACTCGACTGCCTCTTCTCGAAAAGCTGTACCTTGATAACAATAAATTGTCACTTTTGCCTTCTGAGCTTGGTGAACTGAAAAGCTTAAAAGTCCTCAGGGTAGACTACAACATGCTGGTTTCAGTACCTTTAGAGCTGAGGCAGTGTGTTGGATTGGTGGAACTTTCATTGGAACATAACAAGCTTGTCCGGCCTTTGCTTGATTTCAGGGCTATGGCTGAGCTACGAGTTCTTAGGCTGTTTGGAAACCCTCTTGAATTTCTTCCTGAAATCTTGCCACTCCACAAACTTCATCATTTGTCTCTTGCGAATATCAGGATTGTGGCAGATGATAACTTGAGATCGGTGAACGTGCAAATAGAGATGGAAAACAGTTCTTATTTTGGCGCATCCAGGCATAAGCTAAGCGCCTTTTTCTCTCTTATATTTCGGTTTTCTTCTTGTCATCACCCTTTACTAGCATCTGCACTTGCAAAGATTATGCAAGACCAAGGAAACCGCGTAGTTGTTGGCAAAGATGAGAATGCAGTGAGGCAGCTTATAAGTATGATAAGCAGTGACAACCGTCATGTGGTTGAAAAAGCATGTTCTGCTCTTTCATCTCTTGCTGCAGATGTTTCAGTTGCCATGCAGTTGATGAAATCTGACATTATGCAACCCATCGAAACGGTTTTGAAGTCTATGCCCCAGGGGGAAGTAATTTCTGTGTTGCAAGTTGTGGTGAAGCTGGCTTTTGCATCTGATGCTGTAGCTCAGAAGATGCTGACCAAGGATGTATTGAAGTCTTTGAAATTGTTGTGTGCCCTTAAAACCCCTGAGGTACAAAGATTAGCTTTGCTGGCAGTAGGTAATTTGGCCTTTTGTCTGGAGAATCGCCGTCTTTTGGTTACTTCTGAAAGCTTGTGTGAACTTCTAATGCGCTTGACGGCTGCACCTGAGCCCCGTGTGCATAAAGCTGCAGCTCGTGCTTTAGCAATTCTCGGGGAGAATGGAATCCTGCGACGTGCCATAAGGGGGAGACCGGTGCCAAAGCAAGGACTGCGCATACTTTCAATGGATGGTGGTGGTATGAAAGGTCTGGCAACTGTGCAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACAGATACATGAGTTGTTTGACCTCATATGTGGCACATCAACGGGTGGAATGCTTGCTGTTGCGCTTGGCATTAAGCTAATGTCTTTAGACCAATGTGAAGAAATATACAAAAACCTTGGAAAACTTGTCTTTGCTGAACCTGCGCCAAAGGATAATGAAGCTGCAAGTTGGAGAGAGAAGTTGGATCAGCTCTATAAAAGTTCGTCCCAGAGTTTTAGAGTTGTTGTACATGGATCTAAACATAGTGCAGATCAGTTTGAGAGATTGTTGAAGGAAATGTGTGCCGATGAGGATGGGGATCTGTTAATAGAGTCCGCAGTTAAAAACGTTCCCAAAGTTTTTGTTGTGTCAACTTTGGTGAGCGTGATGCCAGCTCAGCCTTTCTTATTCCGAAATTATCAGTATCCTGCGGGAACACTAGAAGTGCCTCTTTCAGAGAGTTCAGGAATAACTGGACCACCTCCTGGATGTGCTGAAGGTGGCTATAAGCACAGTGCCTTTATAGGAAGTTGTAAGCATCAAGTATGGCAAGCTATAAGAGCGTCATCTGCTGCTCCGTATTATCTTGATGATTTCTCAGATGATGTAAATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTTCCATTAGAGAAGCACAACTTTTATGGCCAGACACAAAAATTGACTGCTTAGTTTCCGTTGGCTGTGGTGATGTTCCAACAAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGTCAGGTATTGATAGAAAGTGCATGCTCTGTGGAACGGGTGGAGGAAGCTTTGAGCACATTGCTACCCATGCTCCCTGGAATACAATATTTTCGGTTTAATCCAGTTGATGAACGCTGTGATATGGAACTGGATGAGACTGATCCTGCAGTCTGGCTGAAATTGGAAGATGCAGTTGAGGAATATATTCAGAAAAATTCTCTTGTACTTAAGGATGCCTGTGAGAGACTACTTATGCCCTTTCAACATGATGAGAAGTGGTCACATGTCCCCAAGTCAAAGGCATCAAATGACGAGGAAAAAAGTCCATCTCTAGGTTGGAGGCGCAATGTACTACTTGTCGAAGCTTCGCATAGCCCCAGTTCTGGTCGCACTCAGAACCATGCTCATGCGCTTGAGTCATTTTGTGCTCGTAATGGAATACGATTATCGCAAATGCAAGGCATATCAGGATTTGTGAAAACGGTACCAGCAACAACATTCCCAACGCCGTTTGCATCACCTTTATTTCCTCCAAGCATTCCATCAAGTCCACTTTTCTACAGTCCTGATTTTGGCCCCCAGAGGGTTGGCCGGATCGATATGGTCCCACCTTTAAGCTTAGATGGACAGTCTGGAAAAGGAGCTGCATCACCACCTGATTCTCCTTCAGGACCTAGACAGCTTTCTTTACCCGTCCAGTCATTGCACGAGAAGTTACAGAACTCGCCCCAAGTGGGAATTGTACATTTAGCCCTTCAAAATGACTCTCTTGGCTCGATATTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAGCCTGGAGAGCTTGCAGATAAGTTTCTTCAGAGTGTTAAACTGAGTTTGATATCAGTGGTGCGAAACCGCCGCAGGAAGGCTGATTCATCTTTCGCCAATATTTCAACTATTTCTGATTTGGTTGCATGTAGACCATACTTCCAAATTGGAGGCATCGTTCACCGTTATATGGGACGCCAAACTCAAGTTATGGACGATGGCCAAGAAATTGGAGCATATTTGTTTCGTAGAACTGTGCCTTCTATCCATTTAACACCTGATGATGTTCGCTGGATGGTCGGAGCTTGGAGAGATAGGATCATTATTTGCACAGGGACATATGGACCTACGCCACCACTGATTAAAGCATTTTTAGACACTGGCGCTAAAGCTGTTATAAGTTCTTTAGCTCAGCCCCCAGAAGCACAGCTGACAGCAGTCCATGGATCTGCAGAGTTCAGTGTTTTTGAAAACGGGAAGTTTGAGATTGGAGAGGAAGAGGCCGAAGATGAAGTCGAAGATGAGGATGCTGAGCCTACCAGTCCGGTGAGTGACTGGGAAGATAGTGAAAACGGGGACCGGTCTACAGGTTTTTGGGATGACGATGAGGAAGAGGTGTCGCAATTTGTTTGTCAATTGTACGAGTCACTGTTTCGAGAGGGTGTGAGCGTGGATGTTGCTCTACGACAAGCTCTTGCCTCGCACCGGAAGTTGAGGTATTCATGCCATCTTCCCAGTATACACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm LRR_8 Patatin

Protein Analysis

1315

Amino Acids

144.64

Weight (kDa)

5.58

Isoelectric Point (pI)

50.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	168 - 263	3.9e-06	Leucine-rich repeat region
Patatin	PF01734	531 - 766	1.1e-19	Patatin-like phospholipase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61850 AT1G61850
fragaria_vesca	FvH4_3g02460 FvH4_3g02460 FvH4_3g02460 FvH4_3g02460 FvH4_3g02460
malus_domestica	MD05G1342900.v1.1 MD10G1317800.v1.1
prunus_persica	Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1 Prupe.4G024000_v2.0.a1
pyrus_communis	pycom05g31210 pycom05g31230 pycom10g26910 pycom10g26930
rosa_chinensis	RchiOBHm_Chr5g0003711
rosa_laevigata	RLG00000031168
rosa_multiflora	Rmu_co8519183.1_g000001 Rmu_sc0000500.1_g000003
rosa_roxburghii	Rroxscaffold_1G00072250
rosa_rugosa	Rorug04G0406100 Rorug04G0406200
rosa_samantha	Rh5AG033000 Rh5BG031600 Rh5CG035700 Rh5DG032200
rosa_wichuraiana	Rw5G003050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1085, 3596
AarI	CACCTGC	1 cut(s)	190
AasI	GACNNNNNNGTC	1 cut(s)	370
Acc16I	TGCGCA	1 cut(s)	1586
Acc36I	ACCTGC	2 cut(s)	190, 1816
Acc65I	GGTACC	1 cut(s)	2887
AccB1I	GGYRCC	3 cut(s)	62, 1567, 2887
AccB7I	CCANNNNNTGG	1 cut(s)	2132
AccI	GTMKAC	4 cut(s)	645, 3349, 3782, 3937
AccII	CGCG	1 cut(s)	1044
AciI	CCGC	9 cut(s)	25, 74, 464, 471, 1042, 1983, 2078, 3286, 3289
AclI	AACGTT	1 cut(s)	1995
AclWI	GGATC	9 cut(s)	150, 170, 1860, 1909, 1974, 2558, 3005, 3524, 3652
AcoI	YGGCCR	5 cut(s)	366, 376, 2330, 2992, 3418
AcsI	RAATTY	3 cut(s)	800, 1635, 2617
AcuI	CTGAAG	3 cut(s)	2163, 3054, 3230
AcyI	GRCGYC	2 cut(s)	104, 3394
AflII	CTTAAG	1 cut(s)	2630
AflIII	ACRYGT	1 cut(s)	2686
AgeI	ACCGGT	3 cut(s)	532, 1564, 3777
AhdI	GACNNNNNGTC	1 cut(s)	94
AjiI	CACGTC	1 cut(s)	1547
AjnI	CCWGG	5 cut(s)	935, 1230, 2131, 2497, 3220
AjuI	GAANNNNNNNTTGG	4 cut(s)	14, 46, 2386, 2418
AleI	CACNNNNGTG	1 cut(s)	260
AloI	GAACNNNNNNTCC	2 cut(s)	3360, 3392
Alw21I	GWGCWC	3 cut(s)	54, 2480, 2833
Alw26I	GTCTC	5 cut(s)	74, 849, 1555, 2552, 2641
AlwI	GGATC	9 cut(s)	150, 170, 1860, 1909, 1974, 2558, 3005, 3524, 3652
Ama87I	CYCGRG	1 cut(s)	1523
ApoI	RAATTY	3 cut(s)	800, 1635, 2617
ArsI	GACNNNNNNTTYG	2 cut(s)	3696, 3728
AsiGI	ACCGGT	3 cut(s)	532, 1564, 3777
Asp700I	GAANNNNTTC	2 cut(s)	804, 2607
Asp718I	GGTACC	1 cut(s)	2887
AsuC2I	CCSGG	1 cut(s)	370
AvaI	CYCGRG	1 cut(s)	1523
AvaII	GGWCC	8 cut(s)	71, 276, 372, 2123, 3007, 3074, 3542, 3775
AxyI	CCTNAGG	3 cut(s)	249, 638, 1359
BaeGI	GKGCMC	1 cut(s)	1348
BaeI	ACNNNNGTAYC	2 cut(s)	1664, 1697
BalI	TGGCCA	2 cut(s)	2332, 3420
BanI	GGYRCC	3 cut(s)	62, 1567, 2887
BanII	GRGCYC	1 cut(s)	1485
BauI	CACGAG	3 cut(s)	47, 1506, 3110
BbsI	GAAGAC	1 cut(s)	117
Bbv12I	GWGCWC	3 cut(s)	54, 2480, 2833
BbvCI	CCTCAGC	1 cut(s)	680
BceAI	ACGGC	7 cut(s)	76, 322, 381, 472, 1404, 1485, 2896
BciT130I	CCWGG	5 cut(s)	937, 1232, 2133, 2499, 3222
BciVI	GTATCC	2 cut(s)	2082, 2410
BcnI	CCSGG	1 cut(s)	370
BcoDI	GTCTC	5 cut(s)	74, 849, 1555, 2552, 2641
BfaI	CTAG	5 cut(s)	1001, 2087, 2735, 3078, 3943
BfmI	CTRYAG	8 cut(s)	444, 1152, 1290, 1500, 2568, 2962, 3648, 3783
BfoI	RGCGCY	2 cut(s)	954, 3585
BfrI	CTTAAG	1 cut(s)	2630
BfuAI	ACCTGC	2 cut(s)	190, 1816
BfuI	GTATCC	2 cut(s)	2082, 2410
BglII	AGATCT	1 cut(s)	36
BlpI	GCTNAGC	6 cut(s)	762, 947, 2043, 3216, 3609, 3729
Bme1390I	CCNGG	6 cut(s)	370, 937, 1232, 2133, 2499, 3222
Bme18I	GGWCC	8 cut(s)	71, 276, 372, 2123, 3007, 3074, 3542, 3775
BmeRI	GACNNNNNGTC	1 cut(s)	94
BmeT110I	CYCGRG	1 cut(s)	1523
BmgBI	CACGTC	1 cut(s)	1547
BmiI	GGNNCC	9 cut(s)	64, 72, 215, 373, 1569, 2889, 2980, 3009, 3776
BmrFI	CCNGG	6 cut(s)	370, 937, 1232, 2133, 2499, 3222
BmrI	ACTGGG	4 cut(s)	23, 2775, 3763, 3927
BmuI	ACTGGG	4 cut(s)	23, 2775, 3763, 3927
BoxI	GACNNNNGTC	2 cut(s)	1107, 3635
BpiI	GAAGAC	1 cut(s)	117
BpmI	CTGGAG	4 cut(s)	200, 417, 1430, 3243
Bpu10I	CCTNAGC	2 cut(s)	680, 1479
Bpu1102I	GCTNAGC	6 cut(s)	762, 947, 2043, 3216, 3609, 3729
BpuEI	CTTGAG	3 cut(s)	901, 2837, 3387
BpuMI	CCSGG	1 cut(s)	370
Bsa29I	ATCGAT	1 cut(s)	3000
BsaAI	YACGTR	1 cut(s)	57
BsaBI	GATNNNNATC	2 cut(s)	1965, 2256
BsaHI	GRCGYC	2 cut(s)	104, 3394
BsaI	GGTCTC	2 cut(s)	74, 1555
BsaWI	WCCGGW	5 cut(s)	532, 1564, 3742, 3777, 3903
BsaXI	ACNNNNNCTCC	4 cut(s)	2478, 2508, 3360, 3390
Bse118I	RCCGGY	3 cut(s)	532, 1564, 3777
Bse21I	CCTNAGG	3 cut(s)	249, 638, 1359
Bse3DI	GCAATG	3 cut(s)	2480, 2755, 3104
Bse8I	GATNNNNATC	2 cut(s)	1965, 2256
BseBI	CCWGG	5 cut(s)	937, 1232, 2133, 2499, 3222
BseCI	ATCGAT	1 cut(s)	3000
BseJI	GATNNNNATC	2 cut(s)	1965, 2256
BseMI	GCAATG	3 cut(s)	2480, 2755, 3104
BseRI	GAGGAG	2 cut(s)	209, 296
BseSI	GKGCMC	1 cut(s)	1348
BseX3I	CGGCCG	1 cut(s)	366
BseYI	CCCAGC	1 cut(s)	182
BsgI	GTGCAG	3 cut(s)	993, 1458, 1935
Bsh1236I	CGCG	1 cut(s)	1044
Bsh1285I	CGRYCG	2 cut(s)	91, 369
BshNI	GGYRCC	3 cut(s)	62, 1567, 2887
BshTI	ACCGGT	3 cut(s)	532, 1564, 3777
BshVI	ATCGAT	1 cut(s)	3000
BsiEI	CGRYCG	2 cut(s)	91, 369
BsiHKAI	GWGCWC	3 cut(s)	54, 2480, 2833
BsiHKCI	CYCGRG	1 cut(s)	1523
BsiWI	CGTACG	2 cut(s)	57, 92
BslFI	GGGAC	8 cut(s)	24, 200, 1858, 2675, 2993, 3405, 3547, 3788
BsmAI	GTCTC	5 cut(s)	74, 849, 1555, 2552, 2641
BsmFI	GGGAC	8 cut(s)	24, 200, 1858, 2675, 2993, 3405, 3547, 3788
BsmI	GAATGC	3 cut(s)	1072, 1722, 2940
Bso31I	GGTCTC	2 cut(s)	74, 1555
BsoBI	CYCGRG	1 cut(s)	1523
Bsp1286I	GDGCHC	5 cut(s)	54, 1348, 1485, 2480, 2833
Bsp1407I	TGTACA	2 cut(s)	1894, 3145
Bsp1720I	GCTNAGC	6 cut(s)	762, 947, 2043, 3216, 3609, 3729
Bsp19I	CCATGG	1 cut(s)	3640
BspACI	CCGC	9 cut(s)	25, 74, 464, 471, 1042, 1983, 2078, 3286, 3289
BspDI	ATCGAT	1 cut(s)	3000
BspFNI	CGCG	1 cut(s)	1044
BspLI	GGNNCC	9 cut(s)	64, 72, 215, 373, 1569, 2889, 2980, 3009, 3776
BspMAI	CTGCAG	5 cut(s)	448, 1156, 1504, 2572, 3652
BspMI	ACCTGC	2 cut(s)	190, 1816
BspPI	GGATC	9 cut(s)	150, 170, 1860, 1909, 1974, 2558, 3005, 3524, 3652
BspT107I	GGYRCC	3 cut(s)	62, 1567, 2887
BspTI	CTTAAG	1 cut(s)	2630
BspTNI	GGTCTC	2 cut(s)	74, 1555
BsrDI	GCAATG	3 cut(s)	2480, 2755, 3104
BsrFI	RCCGGY	3 cut(s)	532, 1564, 3777
BsrGI	TGTACA	2 cut(s)	1894, 3145
BssAI	RCCGGY	3 cut(s)	532, 1564, 3777
BssNAI	GTATAC	1 cut(s)	3938
BssNI	GRCGYC	2 cut(s)	104, 3394
BssSI	CACGAG	3 cut(s)	47, 1506, 3110
BssT1I	CCWWGG	5 cut(s)	30, 1033, 1311, 1783, 3640
Bst1107I	GTATAC	1 cut(s)	3938
Bst2BI	CACGAG	3 cut(s)	47, 1506, 3110
Bst2UI	CCWGG	5 cut(s)	937, 1232, 2133, 2499, 3222
Bst6I	CTCTTC	3 cut(s)	561, 3690, 3804
BstACI	GRCGYC	2 cut(s)	104, 3394
BstAFI	CTTAAG	1 cut(s)	2630
BstAPI	GCANNNNNTGC	4 cut(s)	1133, 1286, 1508, 1582
BstAUI	TGTACA	2 cut(s)	1894, 3145
BstBAI	YACGTR	1 cut(s)	57
BstDSI	CCRYGG	3 cut(s)	129, 495, 3640
BstFNI	CGCG	1 cut(s)	1044
BstH2I	RGCGCY	2 cut(s)	954, 3585
BstMAI	GTCTC	5 cut(s)	74, 849, 1555, 2552, 2641
BstMCI	CGRYCG	2 cut(s)	91, 369
BstNI	CCWGG	5 cut(s)	937, 1232, 2133, 2499, 3222
BstNSI	RCATGY	5 cut(s)	658, 1131, 2448, 2690, 3348
BstPAI	GACNNNNGTC	2 cut(s)	1107, 3635
BstSCI	CCNGG	6 cut(s)	368, 935, 1230, 2131, 2497, 3220
BstSFI	CTRYAG	8 cut(s)	444, 1152, 1290, 1500, 2568, 2962, 3648, 3783
BstSLI	GKGCMC	1 cut(s)	1348
BstUI	CGCG	1 cut(s)	1044
BstV2I	GAAGAC	1 cut(s)	117
BstX2I	RGATCY	4 cut(s)	36, 1901, 1966, 3644
BstXI	CCANNNNNNTGG	2 cut(s)	2990, 3428
BstYI	RGATCY	4 cut(s)	36, 1901, 1966, 3644
BstZ17I	GTATAC	1 cut(s)	3938
BstZI	CGGCCG	1 cut(s)	366
Bsu15I	ATCGAT	1 cut(s)	3000
Bsu36I	CCTNAGG	3 cut(s)	249, 638, 1359
BsuI	GTATCC	2 cut(s)	2082, 2410
BsuTUI	ATCGAT	1 cut(s)	3000
BtgI	CCRYGG	3 cut(s)	129, 495, 3640
BtgZI	GCGATG	1 cut(s)	402
BtrI	CACGTC	1 cut(s)	1547
BtsI	GCAGTG	4 cut(s)	491, 692, 1077, 1105
BveI	ACCTGC	2 cut(s)	190, 1816
Cfr10I	RCCGGY	3 cut(s)	532, 1564, 3777
ClaI	ATCGAT	1 cut(s)	3000
CseI	GACGC	5 cut(s)	93, 145, 478, 2199, 3402
CspAI	ACCGGT	3 cut(s)	532, 1564, 3777
CspCI	CAANNNNNGTGG	2 cut(s)	490, 525
DrdI	GACNNNNNNGTC	1 cut(s)	370
DriI	GACNNNNNGTC	1 cut(s)	94
DseDI	GACNNNNNNGTC	1 cut(s)	370
EaeI	YGGCCR	5 cut(s)	366, 376, 2330, 2992, 3418
EagI	CGGCCG	1 cut(s)	366
Eam1104I	CTCTTC	3 cut(s)	561, 3690, 3804
Eam1105I	GACNNNNNGTC	1 cut(s)	94
EarI	CTCTTC	3 cut(s)	561, 3690, 3804
EciI	GGCGGA	1 cut(s)	63
EclXI	CGGCCG	1 cut(s)	366
Eco130I	CCWWGG	5 cut(s)	30, 1033, 1311, 1783, 3640
Eco24I	GRGCYC	1 cut(s)	1485
Eco31I	GGTCTC	2 cut(s)	74, 1555
Eco32I	GATATC	1 cut(s)	3270
Eco47I	GGWCC	8 cut(s)	71, 276, 372, 2123, 3007, 3074, 3542, 3775
Eco52I	CGGCCG	1 cut(s)	366
Eco57I	CTGAAG	3 cut(s)	2163, 3054, 3230
Eco81I	CCTNAGG	3 cut(s)	249, 638, 1359
Eco88I	CYCGRG	1 cut(s)	1523
EcoO109I	RGGNCCY	1 cut(s)	3074
EcoRII	CCWGG	5 cut(s)	935, 1230, 2131, 2497, 3220
EcoRV	GATATC	1 cut(s)	3270
EcoT14I	CCWWGG	5 cut(s)	30, 1033, 1311, 1783, 3640
EcoT38I	GRGCYC	1 cut(s)	1485
ErhI	CCWWGG	5 cut(s)	30, 1033, 1311, 1783, 3640
FalI	AAGNNNNNCTT	8 cut(s)	14, 46, 1360, 1392, 2082, 2114, 2307, 2339
FaqI	GGGAC	8 cut(s)	24, 200, 1858, 2675, 2993, 3405, 3547, 3788
FauI	CCCGC	1 cut(s)	2071
FauNDI	CATATG	2 cut(s)	1694, 3538
FblI	GTMKAC	4 cut(s)	645, 3349, 3782, 3937
FriOI	GRGCYC	1 cut(s)	1485
FspBI	CTAG	5 cut(s)	1001, 2087, 2735, 3078, 3943
FspI	TGCGCA	1 cut(s)	1586
GsaI	CCCAGC	1 cut(s)	186
GsuI	CTGGAG	4 cut(s)	200, 417, 1430, 3243
HaeII	RGCGCY	2 cut(s)	954, 3585
HgaI	GACGC	5 cut(s)	93, 145, 478, 2199, 3402
Hin1I	GRCGYC	2 cut(s)	104, 3394
HincII	GTYRAC	1 cut(s)	2019
HindII	GTYRAC	1 cut(s)	2019
HindIII	AAGCTT	6 cut(s)	625, 725, 1441, 2469, 2766, 3019
Hpy99I	CGWCG	7 cut(s)	100, 103, 109, 118, 139, 458, 1548
HpyAV	CCTTC	9 cut(s)	94, 612, 1648, 1933, 2138, 3078, 3167, 3289, 3468
HpyCH4IV	ACGT	5 cut(s)	56, 95, 894, 1546, 1995
HpySE526I	ACGT	5 cut(s)	56, 95, 894, 1546, 1995
Hsp92I	GRCGYC	2 cut(s)	104, 3394
KpnI	GGTACC	1 cut(s)	2891
LmnI	GCTCC	8 cut(s)	196, 219, 425, 2227, 2499, 3044, 3431, 3500
MaeI	CTAG	5 cut(s)	1001, 2087, 2735, 3078, 3943
MaeII	ACGT	5 cut(s)	56, 95, 894, 1546, 1995
MaeIII	GTNAC	9 cut(s)	349, 490, 591, 1100, 1429, 2682, 3117, 3749, 3843
MfeI	CAATTG	2 cut(s)	1647, 3833
MflI	RGATCY	4 cut(s)	36, 1901, 1966, 3644
MhlI	GDGCHC	5 cut(s)	54, 1348, 1485, 2480, 2833
MlsI	TGGCCA	2 cut(s)	2332, 3420
MluNI	TGGCCA	2 cut(s)	2332, 3420
MlyI	GAGTC	6 cut(s)	400, 408, 1988, 2828, 3161, 3851
MmeI	TCCRAC	6 cut(s)	673, 1817, 1830, 2405, 2720, 3478
Mox20I	TGGCCA	2 cut(s)	2332, 3420
MroXI	GAANNNNTTC	2 cut(s)	804, 2607
MscI	TGGCCA	2 cut(s)	2332, 3420
MslI	CAYNNNNRTG	3 cut(s)	260, 317, 2808
Msp20I	TGGCCA	2 cut(s)	2332, 3420
MspA1I	CMGCKG	1 cut(s)	3628
MspCI	CTTAAG	1 cut(s)	2630
MspR9I	CCNGG	6 cut(s)	370, 937, 1232, 2133, 2499, 3222
MunI	CAATTG	2 cut(s)	1647, 3833
Mva1269I	GAATGC	3 cut(s)	1072, 1722, 2940
MvaI	CCWGG	5 cut(s)	937, 1232, 2133, 2499, 3222
MvnI	CGCG	1 cut(s)	1044
NciI	CCSGG	1 cut(s)	370
NcoI	CCATGG	1 cut(s)	3640
NdeI	CATATG	2 cut(s)	1694, 3538
NlaIV	GGNNCC	9 cut(s)	64, 72, 215, 373, 1569, 2889, 2980, 3009, 3776
NmuCI	GTSAC	7 cut(s)	349, 490, 591, 1100, 2682, 3749, 3843
NsbI	TGCGCA	1 cut(s)	1586
NspI	RCATGY	5 cut(s)	658, 1131, 2448, 2690, 3348
OliI	CACNNNNGTG	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	2448
PaqCI	CACCTGC	1 cut(s)	190
PasI	CCCWGGG	1 cut(s)	1231
PciI	ACATGT	1 cut(s)	2686
PcsI	WCGNNNNNNNCGW	4 cut(s)	101, 104, 113, 119
PctI	GAATGC	3 cut(s)	1072, 1722, 2940
PdmI	GAANNNNTTC	2 cut(s)	804, 2607
PfeI	GAWTC	5 cut(s)	149, 1414, 1536, 3062, 3302
Pfl23II	CGTACG	2 cut(s)	57, 92
PflMI	CCANNNNNTGG	1 cut(s)	2132
PfoI	TCCNGGA	1 cut(s)	2131
PinAI	ACCGGT	3 cut(s)	532, 1564, 3777
PleI	GAGTC	6 cut(s)	400, 407, 1987, 2827, 3161, 3850
PpsI	GAGTC	6 cut(s)	400, 407, 1987, 2827, 3161, 3850
Ppu21I	YACGTR	1 cut(s)	57
PpuMI	RGGWCCY	1 cut(s)	3074
PscI	ACATGT	1 cut(s)	2686
PshAI	GACNNNNGTC	2 cut(s)	1107, 3635
PsiI	TTATAA	2 cut(s)	1085, 3596
Psp1406I	AACGTT	1 cut(s)	1995
Psp5II	RGGWCCY	1 cut(s)	3074
Psp6I	CCWGG	5 cut(s)	935, 1230, 2131, 2497, 3220
PspFI	CCCAGC	1 cut(s)	182
PspGI	CCWGG	5 cut(s)	935, 1230, 2131, 2497, 3220
PspLI	CGTACG	2 cut(s)	57, 92
PspN4I	GGNNCC	9 cut(s)	64, 72, 215, 373, 1569, 2889, 2980, 3009, 3776
PspPPI	RGGWCCY	1 cut(s)	3074
PstI	CTGCAG	5 cut(s)	448, 1156, 1504, 2572, 3652
PsuI	RGATCY	4 cut(s)	36, 1901, 1966, 3644
PvuII	CAGCTG	1 cut(s)	3628
RseI	CAYNNNNRTG	3 cut(s)	260, 317, 2808
SchI	GAGTC	6 cut(s)	400, 408, 1988, 2828, 3161, 3851
ScrFI	CCNGG	6 cut(s)	370, 937, 1232, 2133, 2499, 3222
SduI	GDGCHC	5 cut(s)	54, 1348, 1485, 2480, 2833
SfcI	CTRYAG	8 cut(s)	444, 1152, 1290, 1500, 2568, 2962, 3648, 3783
SinI	GGWCC	8 cut(s)	71, 276, 372, 2123, 3007, 3074, 3542, 3775
SmiMI	CAYNNNNRTG	3 cut(s)	260, 317, 2808
SmlI	CTYRAG	4 cut(s)	880, 2630, 2816, 3402
SmoI	CTYRAG	4 cut(s)	880, 2630, 2816, 3402
SphI	GCATGC	1 cut(s)	2448
SsiI	CCGC	9 cut(s)	25, 74, 464, 471, 1042, 1983, 2078, 3286, 3289
SspI	AATATT	2 cut(s)	2510, 3319
SspMI	CTAG	5 cut(s)	1001, 2087, 2735, 3078, 3943
StyD4I	CCNGG	6 cut(s)	368, 935, 1230, 2131, 2497, 3220
StyI	CCWWGG	5 cut(s)	30, 1033, 1311, 1783, 3640
TaiI	ACGT	5 cut(s)	59, 98, 897, 1549, 1998
TaqII	GACCGA	1 cut(s)	293
TatI	WGTACW	4 cut(s)	1894, 2626, 2752, 3145
TauI	GCSGC	3 cut(s)	28, 467, 3291
TfiI	GAWTC	5 cut(s)	149, 1414, 1536, 3062, 3302
TseFI	GTSAC	7 cut(s)	349, 490, 591, 1100, 2682, 3749, 3843
Tsp45I	GTSAC	7 cut(s)	349, 490, 591, 1100, 2682, 3749, 3843
TspGWI	ACGGA	3 cut(s)	118, 2214, 2350
Van91I	CCANNNNNTGG	1 cut(s)	2132
Vha464I	CTTAAG	1 cut(s)	2630
VpaK11BI	GGWCC	8 cut(s)	71, 276, 372, 2123, 3007, 3074, 3542, 3775
XapI	RAATTY	3 cut(s)	800, 1635, 2617
XceI	RCATGY	5 cut(s)	658, 1131, 2448, 2690, 3348
XcmI	CCANNNNNNNNNTGG	2 cut(s)	386, 2736
XmiI	GTMKAC	4 cut(s)	645, 3349, 3782, 3937
XmnI	GAANNNNTTC	2 cut(s)	804, 2607
XspI	CTAG	5 cut(s)	1001, 2087, 2735, 3078, 3943

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.