Rw5G003050

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004422
Physical Location & Seq
Reverse (-)
2830765 .. 2837653
6889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003050.1

Sequence Viewer

Length: 3966 bp
ATGTCCTGGGGACTGGGATGGAAGCGCCCCTCGGAGATCTTCCATCTCACGCTCTCCTACGGCACCGAGGACCCGCCGACGCCGGAGAATTCCAGCGGCGGCACGCGGTCCTCGGCGTCGTCGTTGGAGTCTTCCTCGTCGGTAGTGTCGCAGCAGGATCAAGAATTGGGGTTCCGGATCGATCTGGATTGGTCCGCCGGCGACGACGAGGACCAGGTGGCGCTGCGGCTCCAGTCGCAGCTGATGGTGGCGCTGCCGATGCCGCAGGACACGGTGGTCGTGGAGCTGAGGCCGGAGGAGGCGAATGTGAGTGTGGATATGAGGGTTGTGCGGCGGAGAGAGCCGTTGAGGGCGGTGAGTATGACCAAGACGGGCGGGTCGGGTCAGCAGAATGACGGCACCGGAGTGTTGACCCGGTTGTTGAGGTCCAATTTCTCTTCTTCCATGCCAGCTGTCGCCGAAGGCGCGGCGGCTTGCGGTGTGCATTGGCAGTGCGTCACGGTGGTCCGTCTCTGTGGTTGTGGTTTGTCGGTATTGCCGGTAGAGCTAACCAGACTGCCTCTTCTTGAGAAGCTACACCTTGATAACAATAAACTGGAACGTTTGCCTTCTGAACTTGGGGAATTGAGAAGCTTAAAGGTGCTCAGGGTTGACTACAACATGCTGGTTTCAGTACCGGTGGAACTGAGACAGTGTGTTGGATTAGTGGAACTTTCTTTGGAACACAACAAGCTTGTTCGGCCTCTGCTTGATTTCAGGGCTATGGCTGAGCTCCGGGTACTTAGGCTATTTGGAAACCCTCTTGAATTTCTTCCTGAAATCTTGCCTCTGCACAAACTTCATCATTTATCTCTCGCAAATATCAGGATTGTGGCAGATGACAGCTTGAGATCAGTGAATGTGCAGATAGAGATGGAAAACAGTTCGTATTTTGGTGCATCTAGGCATAAGCTAAGCGCCTTTTTCGCCCTTATATTCCGTTTTTCTTCTTGTCATCACCCTTTACTAGCATCTGCACTAGCAAAGATAATGCAAGACGAAGGAAACCGTGCAGTCGTTGGTAAAGATGAGAATGCAGTGCGACAGCTTATTAGTATGATCAGTAGTGATGACCGTCATGTGGTTCAAGAAGCATGTTCTGCTCTTTCATCTCTTGCTGCAGACATTTCAGTTGCAATGCAGTTGATGAAAGCCGACATTATGCAACCCATTGAGACGGTACTGAGATCTGTTCTCCAAGAGGAATTGATTTCTGTATTACAAGTTGTGGTGAAGCTGGCTTTTGCATCTGATGCCGTAGCTCAGAAAATGCTGACCAAGGATGTATTGAAGTCGTTAAAACTCTTATGTGCCCACAGAACCCCAGAGGTGCAAAGGTTAGCTTTGGTAGCAGTTGGAAATTTGGCGTTCTGTTTGGAGAATCGTCGCCTTCTGGTTACTTCTGAAAGCTTGTGTGAACTTCTCATGCGCTTAACGGTTGTGCCTGAACCATGTGTGAATAAAGCTGCAGCTCGTGCTTTGGCAATTCTTGGGGAAAATGGAAATCTACGACGTGCCATAAGGGGGAGACAGGTGCCAAAGCAAGGATTACGCATACTCTCAATGGATGGTGGTGGTATGAAAGGTCTGGCAACTGTGCAAATTCTTAAAGCAATTGAGAAGGGAACTGGAAAACCCATACATGAATTGTTTGACCTCATATGCGGCACATCAACTGGTGGCATGCTTGCTGTTGCTCTTGGCATTAAGCTAATGTCATTAGATCAGTGTGAAGAAATATACAAAAACCTTGGAAAACTTGTCTTTGCTGAACCTGCTCCAAAGGACAATGAAGCTGCAAGTTGGAGAGAAAAGTTGGATCAGCTTTATAAAAGTTCGTCGCAGAGTTTCAGAGTTGTTGTACATGGATCTAAACATAGTGCAGATCAGTTTGAGACATTGTTGAAGGAAATGTGTGCTGATGAGGATGGAGATCTGATGATAGAGTCGGCAGTGAAAAACATTCCCAAAGTTTTTGTAGTGTCAACTTTGGTGAGCATGACGCCAGCTCAGCCGTTCTTATTCCGGAATTATCAGTACCCTGCGGGAACGCCAGAAGTAGCTCTTGTAGTTTCAGAGAGTTCAGGAATCACTGTGCAAGAACCAACTACTTTGGGTACTGACCTTGGCTATAAACGCAGTGCTTTTATGGGAAGTTGTAAGCATCAAATATGGCAAGCTATAAGAGCATCATCTGCTGCGCCATACTATCTTGATGATTTCTCAGATGATATTCATCGTTGGCAAGATGGTGCAATAGTGGCAAACAATCCTACAATCTTTGCCATCAGAGAAGCACAACTTTTATGGCCTGACACGAAAATTGATTGCTTAGTTTCTGTTGGCTGTGGTTCTGTTCCGACAAAGGTGCGAAAAGGTGGTTGGCGTTATCTGGATACTGGGCAAGTATTGATAGAGAGTTCTTGCTCTGTGGAACGCGTGGAGGAAGCCTTGAGCACATTGCTACCCATGCTCCCTGGAATACAATACTTTCGGTTTAATCCAGTTGATGAACGTTGTGATATGGAACTGGATGAGACTGATCCAGCTGTCTGGCTGAAATTGGAAGCCTCAGTAGAGGAATATATTCAGAAAAATTCTCTTACTCTTAAGGATGCTTGTGAGAGACTACTGCTGCCATTCCAAAACGACGAAAAGTGGTCCGAGAATTTGAGATCCCAACATTTCCCCAAGGCAAATGAGGTAGATGTGAAAAGCCCATCTCTAGGTTGGAGGCGTAATGTGCTTCTTGTTGAAGCTTCTCATAGCCCCAATTCTGGTCGAGCTTCGAATCACGCTCATGAACTTGAATCATTTTGTGCTCGTAATGGAATACGGGTATCTTTAATGCAAGGAATATCAGGGTTTGTGAAAACCGCAGCAGCAACAACATTCCCAACACCATTTCAGTCGCCTTTGTTTCCTGCAAGCGTCCCATCAAGCCCACTTTTCTACAGTCCTGACTTTGGTCCGCAGAGGGTTGGCCGAATTGATATGGTCCCACCTCTGAGCTTAGATGGCCAACCTGGAAAGGGAGCTGCCTCACCACCAAAGTCTCCTTCAGGACCTAGGCAGCTTTCTGTACCCGTTCAGTCATTGCATGAGAAGTTACAAAATTCGCCACAAGTGGGCATTGTGCATTTGGCCCTTCAAAATGACTCAATTGGCTCGATTTTAAGTTGGCAGAATGATGTATTTGTGGTTGCTGAACCTGGAGAACTTGCAGATAACTTTCTGCAGAGTGTTAAGTTGAGCTTGTTATCAGTTATGCGAAACCATCGCAAGAAGGCTGGATCACCTCTTTCCAATATTTCAACTGTTTCTGATTTGGTTGCTTGTAAACCAATCTTCCAACTTGGATGTATCGTTCACCGTTATATGGGACGCCAAACTCAAGTAATGGAAGATGGCCAAGAAATTGGGGCGTATTTGTTTCGTAGAACTGTCCCCTCTATTCACTTGACACCTGATGATGTTCGATGGATGGTTGGAGCTTGGAGAGACAGGATCATTATTTGTACAGGGACAAATGGGCCCACGCCAACTATGATCAAAGCATTTTTAGACTCTGGTGCCAAAGCGGTTATATCTTCCACAGTACAGCCCCAAGAAACACAGTTAACATTCGGGTCTACTGATTTCAGTGTTCTTGAAAAGTTTGAGATTGGTGATGAGGAGGCTGAAGATGAAGAAGTTGAAGATGAGGCGGCTGAACCTGAAAGTCCGGTGAGTGATTGGGAAGACAGTGAAAATGGAGACCGCTCTATCGGCTTTGGGGATGATGATGAGGAAGAAGTGTCCCAATTCGTCTGTCATTTGTATGACTCACTGTTTCGAGAGGGTACGAGTGTGGATGTTGCTTTACGACAAGCTCTTGCTTCACATCGGAAGTTGAGGTATTTATGTCATCTTCCCAGTATACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001516 GO:0001676 GO:0003674 GO:0003824 GO:0004620 GO:0004623 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0006636 GO:0006644 GO:0006650 GO:0006690 GO:0006692 GO:0006693 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008219 GO:0008610 GO:0009056 GO:0009058 GO:0009395 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009620 GO:0009694 GO:0009695 GO:0009987 GO:0010817 GO:0010876 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0015909 GO:0016020 GO:0016042 GO:0016053 GO:0016298 GO:0016787 GO:0016788 GO:0019369 GO:0019637 GO:0019752 GO:0031090 GO:0031903 GO:0032309 GO:0032787 GO:0033036 GO:0033559 GO:0034638 GO:0042445 GO:0042446 GO:0042579 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043651 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0046337 GO:0046338 GO:0046394 GO:0046434 GO:0046456 GO:0046457 GO:0046470 GO:0046475 GO:0046486 GO:0046503 GO:0046717 GO:0046903 GO:0046942 GO:0047372 GO:0047499 GO:0050482 GO:0050832 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0052689 GO:0065007 GO:0065008 GO:0071702 GO:0071704 GO:0071715 GO:0072330 GO:0097164 GO:0098542 GO:0098588 GO:0098805 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901571 GO:1901575 GO:1901576 GO:1903963
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm LRR_8 Patatin

Protein Analysis

1321

Amino Acids

145.47

Weight (kDa)

5.52

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 170 - 266 5.4e-08 Leucine-rich repeat region
LRR_8 PF13855 188 - 245 4e-07 Leucine rich repeat
Patatin PF01734 533 - 775 6e-20 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1869
AasI GACNNNNNNGTC 2 cut(s) 275, 376
Acc36I ACCTGC 1 cut(s) 1822
AccB1I GGYRCC 4 cut(s) 62, 398, 1573, 3611
AccBSI CCGCTC 1 cut(s) 3801
AccI GTMKAC 2 cut(s) 3671, 3958
AccII CGCG 3 cut(s) 106, 467, 2478
AccIII TCCGGA 2 cut(s) 174, 2064
AclI AACGTT 2 cut(s) 601, 2554
AclWI GGATC 8 cut(s) 165, 185, 1866, 1915, 2576, 2709, 3340, 3554
AcoI YGGCCR 3 cut(s) 3022, 3058, 3448
AcsI RAATTY 7 cut(s) 88, 806, 1399, 1641, 2635, 2707, 3154
AcuI CTGAAG 2 cut(s) 3084, 3741
AcyI GRCGYC 4 cut(s) 80, 116, 2042, 3424
AfiI CCNNNNNNNGG 9 cut(s) 1120, 1563, 1583, 2765, 2816, 3005, 3071, 3167, 3418
AflII CTTAAG 1 cut(s) 2648
AflIII ACRYGT 1 cut(s) 2476
AgeI ACCGGT 1 cut(s) 676
AjiI CACGTC 1 cut(s) 1553
AjnI CCWGG 5 cut(s) 5, 213, 2515, 3064, 3250
AjuI GAANNNNNNNTTGG 4 cut(s) 2404, 2436, 3082, 3114
AleI CACNNNNGTG 1 cut(s) 404
AloI GAACNNNNNNTCC 2 cut(s) 3390, 3422
Alw21I GWGCWC 4 cut(s) 645, 774, 2498, 2863
AlwI GGATC 8 cut(s) 165, 185, 1866, 1915, 2576, 2709, 3340, 3554
Aor13HI TCCGGA 2 cut(s) 174, 2064
AoxI GGCC 8 cut(s) 290, 740, 2348, 3022, 3058, 3183, 3448, 3572
ApaI GGGCCC 1 cut(s) 3576
ApoI RAATTY 7 cut(s) 88, 806, 1399, 1641, 2635, 2707, 3154
AsiGI ACCGGT 1 cut(s) 676
Asp700I GAANNNNTTC 2 cut(s) 810, 2625
AspA2I CCTAGG 1 cut(s) 3107
AspLEI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
AsuC2I CCSGG 2 cut(s) 415, 776
AsuHPI GGTGA 9 cut(s) 367, 989, 1282, 2044, 3075, 3327, 3401, 3719, 3778
AsuII TTCGAA 1 cut(s) 2828
AvrII CCTAGG 1 cut(s) 3107
BaeGI GKGCMC 2 cut(s) 1354, 3576
BaeI ACNNNNGTAYC 2 cut(s) 3873, 3906
BalI TGGCCA 2 cut(s) 3060, 3450
BanI GGYRCC 4 cut(s) 62, 398, 1573, 3611
BanII GRGCYC 2 cut(s) 774, 3576
BauI CACGAG 1 cut(s) 1512
BbsI GAAGAC 2 cut(s) 123, 3786
Bbv12I GWGCWC 4 cut(s) 645, 774, 2498, 2863
BbvCI CCTCAGC 1 cut(s) 287
BceAI ACGGC 5 cut(s) 76, 328, 412, 1280, 2038
BcgI CGANNNNNNTGC 2 cut(s) 2389, 2423
BciT130I CCWGG 5 cut(s) 7, 215, 2517, 3066, 3252
BciVI GTATCC 1 cut(s) 2428
BclI TGATCA 2 cut(s) 1098, 3588
BcnI CCSGG 2 cut(s) 415, 776
BfaI CTAG 5 cut(s) 942, 1007, 1019, 2765, 3108
BfmI CTRYAG 4 cut(s) 1158, 1506, 2992, 3275
BfoI RGCGCY 4 cut(s) 28, 224, 254, 960
BfrI CTTAAG 1 cut(s) 2648
BfuAI ACCTGC 1 cut(s) 1822
BfuI GTATCC 1 cut(s) 2428
BglII AGATCT 3 cut(s) 36, 1226, 1972
BlnI CCTAGG 1 cut(s) 3107
BlpI GCTNAGC 3 cut(s) 768, 953, 2049
Bme1390I CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3066, 3252
BmgBI CACGTC 1 cut(s) 1553
BmiI GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1575, 3039, 3574, 3613
BmrFI CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3066, 3252
BmrI ACTGGG 3 cut(s) 23, 2448, 3948
BmsI GCATC 8 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644
BmuI ACTGGG 3 cut(s) 23, 2448, 3948
BoxI GACNNNNGTC 1 cut(s) 3006
BpiI GAAGAC 2 cut(s) 123, 3786
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 2 cut(s) 215, 3273
Bpu10I CCTNAGC 2 cut(s) 287, 644
Bpu1102I GCTNAGC 3 cut(s) 768, 953, 2049
Bpu14I TTCGAA 1 cut(s) 2828
BpuEI CTTGAG 4 cut(s) 587, 907, 2512, 3417
BpuMI CCSGG 2 cut(s) 415, 776
Bsa29I ATCGAT 1 cut(s) 180
BsaBI GATNNNNATC 2 cut(s) 1971, 2274
BsaHI GRCGYC 4 cut(s) 80, 116, 2042, 3424
BsaI GGTCTC 1 cut(s) 3789
BsaWI WCCGGW 5 cut(s) 174, 401, 676, 2064, 3763
BsaXI ACNNNNNCTCC 2 cut(s) 2496, 2526
Bsc4I CCNNNNNNNGG 9 cut(s) 1120, 1563, 1583, 2765, 2816, 3005, 3071, 3167, 3418
Bse118I RCCGGY 3 cut(s) 197, 538, 676
Bse1I ACTGG 9 cut(s) 18, 232, 600, 1672, 1720, 2443, 2543, 2574, 3954
Bse3DI GCAATG 3 cut(s) 1182, 2498, 3134
Bse8I GATNNNNATC 2 cut(s) 1971, 2274
BseAI TCCGGA 2 cut(s) 174, 2064
BseBI CCWGG 5 cut(s) 7, 215, 2517, 3066, 3252
BseCI ATCGAT 1 cut(s) 180
BseJI GATNNNNATC 2 cut(s) 1971, 2274
BseLI CCNNNNNNNGG 9 cut(s) 1120, 1563, 1583, 2765, 2816, 3005, 3071, 3167, 3418
BseMI GCAATG 3 cut(s) 1182, 2498, 3134
BseNI ACTGG 9 cut(s) 18, 232, 600, 1672, 1720, 2443, 2543, 2574, 3954
BseRI GAGGAG 2 cut(s) 311, 3728
BseSI GKGCMC 2 cut(s) 1354, 3576
BsgI GTGCAG 5 cut(s) 815, 923, 999, 1071, 1941
Bsh1236I CGCG 3 cut(s) 106, 467, 2478
BshFI GGCC 8 cut(s) 292, 742, 2350, 3024, 3060, 3185, 3450, 3574
BshNI GGYRCC 4 cut(s) 62, 398, 1573, 3611
BshTI ACCGGT 1 cut(s) 676
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 4 cut(s) 645, 774, 2498, 2863
BslFI GGGAC 7 cut(s) 24, 2957, 3023, 3435, 3470, 3577, 3823
BslI CCNNNNNNNGG 9 cut(s) 1120, 1563, 1583, 2765, 2816, 3005, 3071, 3167, 3418
BsmBI CGTCTC 2 cut(s) 515, 1208
BsmFI GGGAC 7 cut(s) 24, 2957, 3023, 3435, 3470, 3577, 3823
BsmI GAATGC 1 cut(s) 1078
BsnI GGCC 8 cut(s) 292, 742, 2350, 3024, 3060, 3185, 3450, 3574
Bso31I GGTCTC 1 cut(s) 3789
Bsp119I TTCGAA 1 cut(s) 2828
Bsp120I GGGCCC 1 cut(s) 3572
Bsp1286I GDGCHC 6 cut(s) 645, 774, 1354, 2498, 2863, 3576
Bsp13I TCCGGA 2 cut(s) 174, 2064
Bsp1407I TGTACA 2 cut(s) 1900, 3557
Bsp1720I GCTNAGC 3 cut(s) 768, 953, 2049
BspANI GGCC 8 cut(s) 292, 742, 2350, 3024, 3060, 3185, 3450, 3574
BspDI ATCGAT 1 cut(s) 180
BspEI TCCGGA 2 cut(s) 174, 2064
BspFNI CGCG 3 cut(s) 106, 467, 2478
BspHI TCATGA 1 cut(s) 2839
BspLI GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1575, 3039, 3574, 3613
BspMAI CTGCAG 3 cut(s) 1162, 1510, 3279
BspMI ACCTGC 1 cut(s) 1822
BspPI GGATC 8 cut(s) 165, 185, 1866, 1915, 2576, 2709, 3340, 3554
BspT104I TTCGAA 1 cut(s) 2828
BspT107I GGYRCC 4 cut(s) 62, 398, 1573, 3611
BspTI CTTAAG 1 cut(s) 2648
BspTNI GGTCTC 1 cut(s) 3789
BsrBI CCGCTC 1 cut(s) 3801
BsrDI GCAATG 3 cut(s) 1182, 2498, 3134
BsrFI RCCGGY 3 cut(s) 197, 538, 676
BsrGI TGTACA 2 cut(s) 1900, 3557
BsrI ACTGG 9 cut(s) 18, 232, 600, 1672, 1720, 2443, 2543, 2574, 3954
BssAI RCCGGY 3 cut(s) 197, 538, 676
BssNAI GTATAC 1 cut(s) 3959
BssNI GRCGYC 4 cut(s) 80, 116, 2042, 3424
BssSI CACGAG 1 cut(s) 1512
BssT1I CCWWGG 5 cut(s) 1317, 1789, 2164, 2730, 3107
Bst1107I GTATAC 1 cut(s) 3959
Bst2BI CACGAG 1 cut(s) 1512
Bst2UI CCWGG 5 cut(s) 7, 215, 2517, 3066, 3252
Bst6I CTCTTC 2 cut(s) 442, 567
BstACI GRCGYC 4 cut(s) 80, 116, 2042, 3424
BstAFI CTTAAG 1 cut(s) 2648
BstAPI GCANNNNNTGC 4 cut(s) 1139, 1292, 1514, 2234
BstAUI TGTACA 2 cut(s) 1900, 3557
BstBI TTCGAA 1 cut(s) 2828
BstFNI CGCG 3 cut(s) 106, 467, 2478
BstH2I RGCGCY 4 cut(s) 28, 224, 254, 960
BstHHI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
BstNI CCWGG 5 cut(s) 7, 215, 2517, 3066, 3252
BstNSI RCATGY 3 cut(s) 664, 1137, 1726
BstPAI GACNNNNGTC 1 cut(s) 3006
BstSCI CCNGG 7 cut(s) 5, 213, 413, 774, 2515, 3064, 3250
BstSFI CTRYAG 4 cut(s) 1158, 1506, 2992, 3275
BstSLI GKGCMC 2 cut(s) 1354, 3576
BstUI CGCG 3 cut(s) 106, 467, 2478
BstV2I GAAGAC 2 cut(s) 123, 3786
BstX2I RGATCY 5 cut(s) 36, 1226, 1907, 1972, 2714
BstXI CCANNNNNNTGG 2 cut(s) 2592, 3458
BstYI RGATCY 5 cut(s) 36, 1226, 1907, 1972, 2714
BstZ17I GTATAC 1 cut(s) 3959
Bsu15I ATCGAT 1 cut(s) 180
BsuI GTATCC 1 cut(s) 2428
BsuRI GGCC 8 cut(s) 292, 742, 2350, 3024, 3060, 3185, 3450, 3574
BsuTUI ATCGAT 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 3302
BtrI CACGTC 1 cut(s) 1553
BtsI GCAGTG 4 cut(s) 497, 1083, 1998, 2185
BveI ACCTGC 1 cut(s) 1822
CciI TCATGA 1 cut(s) 2839
CfoI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
Cfr10I RCCGGY 3 cut(s) 197, 538, 676
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 6 cut(s) 88, 105, 484, 2050, 2959, 3432
CsiI ACCWGGT 1 cut(s) 213
CspAI ACCGGT 1 cut(s) 676
DrdI GACNNNNNNGTC 2 cut(s) 275, 376
DseDI GACNNNNNNGTC 2 cut(s) 275, 376
EaeI YGGCCR 3 cut(s) 3022, 3058, 3448
Eam1104I CTCTTC 2 cut(s) 442, 567
EarI CTCTTC 2 cut(s) 442, 567
EciI GGCGGA 2 cut(s) 184, 349
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 5 cut(s) 1317, 1789, 2164, 2730, 3107
Eco24I GRGCYC 2 cut(s) 774, 3576
Eco31I GGTCTC 1 cut(s) 3789
Eco53kI GAGCTC 1 cut(s) 772
Eco57I CTGAAG 2 cut(s) 3084, 3741
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 2 cut(s) 70, 3104
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 5 cut(s) 5, 213, 2515, 3064, 3250
EcoT14I CCWWGG 5 cut(s) 1317, 1789, 2164, 2730, 3107
EcoT38I GRGCYC 2 cut(s) 774, 3576
ErhI CCWWGG 5 cut(s) 1317, 1789, 2164, 2730, 3107
Esp3I CGTCTC 2 cut(s) 515, 1208
FalI AAGNNNNNCTT 8 cut(s) 1366, 1398, 2088, 2120, 2325, 2357, 3278, 3310
FaqI GGGAC 7 cut(s) 24, 2957, 3023, 3435, 3470, 3577, 3823
FauI CCCGC 3 cut(s) 81, 368, 2077
FauNDI CATATG 1 cut(s) 1700
FbaI TGATCA 2 cut(s) 1098, 3588
FblI GTMKAC 2 cut(s) 3671, 3958
FriOI GRGCYC 2 cut(s) 774, 3576
FspBI CTAG 5 cut(s) 942, 1007, 1019, 2765, 3108
GlaI GCGC 7 cut(s) 26, 222, 252, 466, 958, 1469, 2241
GsuI CTGGAG 2 cut(s) 215, 3273
HaeII RGCGCY 4 cut(s) 28, 224, 254, 960
HaeIII GGCC 8 cut(s) 292, 742, 2350, 3024, 3060, 3185, 3450, 3574
HgaI GACGC 6 cut(s) 88, 105, 484, 2050, 2959, 3432
HhaI GCGC 7 cut(s) 27, 223, 253, 467, 959, 1470, 2242
Hin1I GRCGYC 4 cut(s) 80, 116, 2042, 3424
Hin6I GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
HinP1I GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
HincII GTYRAC 4 cut(s) 411, 652, 2025, 3660
HindII GTYRAC 4 cut(s) 411, 652, 2025, 3660
HindIII AAGCTT 4 cut(s) 631, 731, 1447, 2796
HinfI GANTC 9 cut(s) 128, 1420, 1985, 2127, 2830, 2849, 3197, 3605, 3863
HpaI GTTAAC 1 cut(s) 3660
HphI GGTGA 9 cut(s) 367, 989, 1282, 2044, 3075, 3327, 3401, 3719, 3778
Hpy166II GTNNAC 9 cut(s) 411, 652, 1457, 2025, 3380, 3409, 3660, 3672, 3959
Hpy8I GTNNAC 9 cut(s) 411, 652, 1457, 2025, 3380, 3409, 3660, 3672, 3959
HpyAV CCTTC 9 cut(s) 455, 618, 1034, 1439, 1654, 1939, 3108, 3197, 3319
HpyCH4IV ACGT 3 cut(s) 601, 1552, 2554
HpySE526I ACGT 3 cut(s) 601, 1552, 2554
Hsp92I GRCGYC 4 cut(s) 80, 116, 2042, 3424
HspAI GCGC 7 cut(s) 25, 221, 251, 465, 957, 1468, 2240
Kpn2I TCCGGA 2 cut(s) 174, 2064
KroI GCCGGC 1 cut(s) 197
KroNI GCCGGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 1098, 3588
KspAI GTTAAC 1 cut(s) 3660
LmnI GCTCC 7 cut(s) 234, 283, 777, 1822, 2517, 3074, 3530
LweI GCATC 8 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644
MabI ACCWGGT 1 cut(s) 213
MaeI CTAG 5 cut(s) 942, 1007, 1019, 2765, 3108
MaeII ACGT 3 cut(s) 601, 1552, 2554
MaeIII GTNAC 3 cut(s) 496, 1435, 3147
MbiI CCGCTC 1 cut(s) 3801
MfeI CAATTG 2 cut(s) 1653, 3201
MflI RGATCY 5 cut(s) 36, 1226, 1907, 1972, 2714
MhlI GDGCHC 6 cut(s) 645, 774, 1354, 2498, 2863, 3576
MlsI TGGCCA 2 cut(s) 3060, 3450
MluI ACGCGT 1 cut(s) 2476
MluNI TGGCCA 2 cut(s) 3060, 3450
MlyI GAGTC 5 cut(s) 137, 1994, 3191, 3599, 3857
MmeI TCCRAC 9 cut(s) 105, 679, 1375, 1823, 1836, 2423, 2750, 3415, 3508
Mox20I TGGCCA 2 cut(s) 3060, 3450
MreI CGCCGGCG 1 cut(s) 197
MroI TCCGGA 2 cut(s) 174, 2064
MroNI GCCGGC 1 cut(s) 197
MroXI GAANNNNTTC 2 cut(s) 810, 2625
MscI TGGCCA 2 cut(s) 3060, 3450
MslI CAYNNNNRTG 3 cut(s) 404, 2838, 3858
Msp20I TGGCCA 2 cut(s) 3060, 3450
MspA1I CMGCKG 4 cut(s) 96, 241, 452, 2588
MspCI CTTAAG 1 cut(s) 2648
MspR9I CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3066, 3252
MunI CAATTG 2 cut(s) 1653, 3201
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 5 cut(s) 7, 215, 2517, 3066, 3252
MvnI CGCG 3 cut(s) 106, 467, 2478
NaeI GCCGGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 415, 776
NdeI CATATG 1 cut(s) 1700
NgoMIV GCCGGC 1 cut(s) 197
NlaIV GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1575, 3039, 3574, 3613
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 496
NspI RCATGY 3 cut(s) 664, 1137, 1726
NspV TTCGAA 1 cut(s) 2828
OliI CACNNNNGTG 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1726
PagI TCATGA 1 cut(s) 2839
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 1 cut(s) 1078
PdiI GCCGGC 1 cut(s) 199
PdmI GAANNNNTTC 2 cut(s) 810, 2625
PfeI GAWTC 4 cut(s) 1420, 2127, 2830, 2849
PinAI ACCGGT 1 cut(s) 676
PleI GAGTC 5 cut(s) 136, 1993, 3191, 3599, 3857
PpsI GAGTC 5 cut(s) 136, 1993, 3191, 3599, 3857
PpuMI RGGWCCY 2 cut(s) 70, 3104
PshAI GACNNNNGTC 1 cut(s) 3006
PsiI TTATAA 1 cut(s) 1869
Psp124BI GAGCTC 1 cut(s) 774
Psp1406I AACGTT 2 cut(s) 601, 2554
Psp5II RGGWCCY 2 cut(s) 70, 3104
Psp6I CCWGG 5 cut(s) 5, 213, 2515, 3064, 3250
PspGI CCWGG 5 cut(s) 5, 213, 2515, 3064, 3250
PspN4I GGNNCC 9 cut(s) 64, 72, 173, 230, 400, 1575, 3039, 3574, 3613
PspOMI GGGCCC 1 cut(s) 3572
PspPPI RGGWCCY 2 cut(s) 70, 3104
PstI CTGCAG 3 cut(s) 1162, 1510, 3279
PsuI RGATCY 5 cut(s) 36, 1226, 1907, 1972, 2714
PvuII CAGCTG 3 cut(s) 241, 452, 2588
RseI CAYNNNNRTG 3 cut(s) 404, 2838, 3858
SacI GAGCTC 1 cut(s) 774
SchI GAGTC 5 cut(s) 137, 1994, 3191, 3599, 3857
ScrFI CCNGG 7 cut(s) 7, 215, 415, 776, 2517, 3066, 3252
SduI GDGCHC 6 cut(s) 645, 774, 1354, 2498, 2863, 3576
SexAI ACCWGGT 1 cut(s) 213
SfaNI GCATC 8 cut(s) 249, 947, 1019, 1282, 1295, 2212, 2237, 2644
SfcI CTRYAG 4 cut(s) 1158, 1506, 2992, 3275
SfuI TTCGAA 1 cut(s) 2828
SgrAI CRCCGGYG 1 cut(s) 197
SmiMI CAYNNNNRTG 3 cut(s) 404, 2838, 3858
SmlI CTYRAG 5 cut(s) 566, 886, 2491, 2648, 3432
SmoI CTYRAG 5 cut(s) 566, 886, 2491, 2648, 3432
SphI GCATGC 1 cut(s) 1726
SspI AATATT 1 cut(s) 3349
SspMI CTAG 5 cut(s) 942, 1007, 1019, 2765, 3108
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 7 cut(s) 5, 213, 413, 774, 2515, 3064, 3250
StyI CCWWGG 5 cut(s) 1317, 1789, 2164, 2730, 3107
TaiI ACGT 3 cut(s) 604, 1555, 2557
TaqI TCGA 6 cut(s) 180, 2821, 2828, 3209, 3517, 3874
TatI WGTACW 3 cut(s) 1900, 3557, 3637
TauI GCSGC 9 cut(s) 99, 102, 229, 265, 334, 470, 473, 1707, 3749
TfiI GAWTC 4 cut(s) 1420, 2127, 2830, 2849
TseFI GTSAC 1 cut(s) 496
Tsp45I GTSAC 1 cut(s) 496
TspGWI ACGGA 2 cut(s) 497, 968
Vha464I CTTAAG 1 cut(s) 2648
XapI RAATTY 7 cut(s) 88, 806, 1399, 1641, 2635, 2707, 3154
XceI RCATGY 3 cut(s) 664, 1137, 1726
XcmI CCANNNNNNNNNTGG 1 cut(s) 2766
XmaJI CCTAGG 1 cut(s) 3107
XmiI GTMKAC 2 cut(s) 3671, 3958
XmnI GAANNNNTTC 2 cut(s) 810, 2625
XspI CTAG 5 cut(s) 942, 1007, 1019, 2765, 3108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.