FvH4_3g05300

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014072
Physical Location & Seq
Reverse (-)
3089947 .. 3092630
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05300.t1

Sequence Viewer

Length: 1149 bp
ATGGCCAAAACACCACCAACTCGAGTGATTGGTTCGACGAGAGGCTACAAATTTGAAGAACATTATCCTCAAAAACCCTACAAATCTAGTGCAAGACCCACGATTGCATTCTTCTTCTCTATACTCATTTACACTGCCATCTTCTATATTTTCAACCTCTCTCCTTCCATTGATCTCTTCTACAACACCAAGTTCTGGTTTGCTATATCCAACACCCTGATTCTTATCATTGCAGCAGACTACTCTTCTTCCAAAGACAAGCACGACCAAATTACTTACCAAGAAGAGTACATGATGCATAGGCAGCAAGCTCAGACTACTTCCTCATTCGTTTCGGAACCTCCTCGTGAAATAGAAACCATCATTGATCACCCTGTTGCACATCAAAAAATAGAAAACCCAGAAAACAGGCCAACTGTGCTTAAAGACGAGCATGACGAAAAGCCTAGACTTCCTAGTGTTGATGAAATTGATGTCCGAGAAAAGAGCATAGTCCACGTTCAGTCAAAAGGTCGTAAGAAAAAGAATGTTCAAGCAAAAACTTACAAGCGAAGTAAGTCGGAGGAGGTGAGAAGAGTGGTGAAAGACGAATGCAAGAGCAATGATATAAGGAGGTCGGAGACAATGGAGAAGCATGAAGAAACTCAAGCCAGTACTCCGGAAGAAGCTAATGAATTTTCAACCATGTCAAATGAAGAAGTGAACAGAAGATTTGAAGAGTTTATCCAAAGAGACTCTGTTGGCTCTCCCATTGTGTTTGGTGCGCGGAAACTTCACAATTTTTCTGTTTCGTTGGCTGAATGCATAGCTTTAAAAGAAGGGCTCATTGTTGCTAAAAGATGCAACTGTACCCAAATTGAAGTAGAAGGAGACTCTTCCCTTATCATCAACTATGTCAAAGGGACTTGTGCTATTCCTTGGAAACTCAAGTCCCTGATCCGAGATGTTATAGCTCTTGCCGATTCGTTTGTCAACATCACCTTCTCTCATACTTACAGAGAGGCAAACTTTCTTGCGGATGCTCTTGCTTCGGTTGGTCACTCTCTCTCCTCACCGATGTGTTGGATTGATGGGATTCCTCCCCAAGCTCAGATGACTTTTCTTATGGATTGTTCTTGTATTGGTTGTCTAAGAAGCTCTTCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

383

Amino Acids

43.6

Weight (kDa)

6.84

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 243 - 344 6.5e-18 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014072)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30190 AT2G34610
fragaria_vesca FvH4_3g05300 FvH4_6g37202
malus_domestica MD05G1281700.v1.1 MD10G1260500.v1.1
prunus_persica Prupe.4G082900_v2.0.a1
pyrus_communis pycom10g21640
rosa_laevigata RLG00000032108
rosa_multiflora Rmu_ssc0000422.1_g000035
rosa_roxburghii Rroxscaffold_1G00062150
rosa_rugosa Rorug05G0018200
rosa_samantha Rh5AG113000 Rh5BG109700 Rh5CG121500 Rh5DG108800
rosa_wichuraiana Rw5G009800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 195
AccII CGCG 1 cut(s) 766
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 2 cut(s) 766, 1016
AclWI GGATC 1 cut(s) 931
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 50, 674
AfaI GTAC 3 cut(s) 290, 655, 850
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 6 cut(s) 56, 154, 533, 681, 716, 860
AleI CACNNNNGTG 1 cut(s) 1057
AluBI AGCT 6 cut(s) 311, 668, 809, 953, 1088, 1137
AluI AGCT 6 cut(s) 311, 668, 809, 953, 1088, 1137
Alw26I GTCTC 3 cut(s) 614, 726, 864
AlwI GGATC 1 cut(s) 931
Ama87I CYCGRG 1 cut(s) 21
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 2 cut(s) 3, 410
ApeKI GCWGC 2 cut(s) 233, 304
ApoI RAATTY 2 cut(s) 50, 674
Asp700I GAANNNNTTC 1 cut(s) 1138
AspLEI GCGC 1 cut(s) 766
AsuHPI GGTGA 5 cut(s) 362, 580, 592, 970, 1044
AvaI CYCGRG 1 cut(s) 21
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 825
BauI CACGAG 1 cut(s) 345
BbvI GCAGC 2 cut(s) 245, 316
BccI CCATC 3 cut(s) 146, 368, 1064
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 3 cut(s) 614, 726, 864
BfaI CTAG 3 cut(s) 87, 447, 456
BisI GCNGC 2 cut(s) 234, 305
BlsI GCNGC 2 cut(s) 235, 306
BmcAI AGTACT 1 cut(s) 655
BmeT110I CYCGRG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 339
BmsI GCATC 3 cut(s) 285, 830, 1009
BpuEI CTTGAG 2 cut(s) 630, 911
BsaBI GATNNNNATC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 917
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse1I ACTGG 1 cut(s) 651
Bse3DI GCAATG 2 cut(s) 228, 607
Bse8I GATNNNNATC 1 cut(s) 224
BseAI TCCGGA 1 cut(s) 658
BseDI CCNNGG 1 cut(s) 917
BseGI GGATG 1 cut(s) 1024
BseJI GATNNNNATC 1 cut(s) 224
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMI GCAATG 2 cut(s) 228, 607
BseMII CTCAG 2 cut(s) 326, 1103
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 3 cut(s) 333, 578, 1039
BseXI GCAGC 2 cut(s) 245, 316
Bsh1236I CGCG 1 cut(s) 766
BshFI GGCC 2 cut(s) 5, 412
BsiHKCI CYCGRG 1 cut(s) 21
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 2 cut(s) 916, 916
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 3 cut(s) 614, 726, 864
BsmFI GGGAC 2 cut(s) 916, 916
BsmI GAATGC 3 cut(s) 107, 596, 806
BsnI GGCC 2 cut(s) 5, 412
BsoBI CYCGRG 1 cut(s) 21
Bsp1286I GDGCHC 1 cut(s) 825
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 3 cut(s) 172, 367, 936
BspACI CCGC 2 cut(s) 766, 1016
BspANI GGCC 2 cut(s) 5, 412
BspCNI CTCAG 2 cut(s) 325, 1102
BspEI TCCGGA 1 cut(s) 658
BspFNI CGCG 1 cut(s) 766
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 1 cut(s) 931
BspQI GCTCTTC 1 cut(s) 1144
BsrDI GCAATG 2 cut(s) 228, 607
BsrI ACTGG 1 cut(s) 651
BssECI CCNNGG 1 cut(s) 917
BssMI GATC 3 cut(s) 172, 367, 936
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 917
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 2 cut(s) 418, 848
Bst6I CTCTTC 7 cut(s) 182, 250, 279, 568, 711, 880, 1144
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 312, 1089, 1130
BstF5I GGATG 1 cut(s) 1024
BstFNI CGCG 1 cut(s) 766
BstHHI GCGC 1 cut(s) 766
BstKTI GATC 3 cut(s) 175, 370, 939
BstMAI GTCTC 3 cut(s) 614, 726, 864
BstMBI GATC 3 cut(s) 172, 367, 936
BstMWI GCNNNNNNNGC 2 cut(s) 304, 418
BstUI CGCG 1 cut(s) 766
BstV1I GCAGC 2 cut(s) 245, 316
BsuRI GGCC 2 cut(s) 5, 412
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 1 cut(s) 766
Csp6I GTAC 3 cut(s) 289, 654, 849
CviAII CATG 4 cut(s) 292, 434, 635, 685
CviQI GTAC 3 cut(s) 289, 654, 849
DdeI CTNAG 3 cut(s) 312, 1089, 1130
DpnI GATC 3 cut(s) 174, 369, 938
DpnII GATC 3 cut(s) 172, 367, 936
DraI TTTAAA 1 cut(s) 813
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 7 cut(s) 182, 250, 279, 568, 711, 880, 1144
EarI CTCTTC 7 cut(s) 182, 250, 279, 568, 711, 880, 1144
Eco130I CCWWGG 1 cut(s) 917
Eco24I GRGCYC 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 917
EcoT22I ATGCAT 2 cut(s) 300, 806
EcoT38I GRGCYC 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 917
FaeI CATG 4 cut(s) 295, 437, 638, 688
FalI AAGNNNNNCTT 1 cut(s) 1123
FaqI GGGAC 2 cut(s) 916, 916
FatI CATG 4 cut(s) 291, 433, 634, 684
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 234, 305
FokI GGATG 1 cut(s) 1031
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 2 cut(s) 234, 305
FspBI CTAG 3 cut(s) 87, 447, 456
GlaI GCGC 1 cut(s) 765
GluI GCNGC 2 cut(s) 234, 305
HaeIII GGCC 2 cut(s) 5, 412
HapII CCGG 1 cut(s) 659
HhaI GCGC 1 cut(s) 766
Hin1II CATG 4 cut(s) 295, 437, 638, 688
Hin6I GCGC 1 cut(s) 764
HinP1I GCGC 1 cut(s) 764
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HinfI GANTC 5 cut(s) 220, 734, 872, 962, 1075
HpaII CCGG 1 cut(s) 659
HphI GGTGA 5 cut(s) 362, 580, 592, 970, 1044
Hpy166II GTNNAC 3 cut(s) 496, 703, 973
Hpy188I TCNGA 7 cut(s) 315, 337, 479, 562, 619, 941, 1092
Hpy188III TCNNGA 2 cut(s) 347, 659
Hpy8I GTNNAC 3 cut(s) 496, 703, 973
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 4 cut(s) 174, 812, 860, 991
HpyCH4III ACNGT 2 cut(s) 418, 848
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 8 cut(s) 92, 107, 233, 298, 380, 594, 804, 843
HpyF10VI GCNNNNNNNGC 2 cut(s) 304, 418
HpyF3I CTNAG 3 cut(s) 312, 1089, 1130
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 4 cut(s) 295, 437, 638, 688
HspAI GCGC 1 cut(s) 764
Kpn2I TCCGGA 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 3 cut(s) 172, 367, 936
LguI GCTCTTC 1 cut(s) 1144
LpnPI CCDG 8 cut(s) 181, 230, 387, 394, 414, 664, 672, 947
Lsp1109I GCAGC 2 cut(s) 245, 316
LweI GCATC 3 cut(s) 285, 830, 1009
MaeI CTAG 3 cut(s) 87, 447, 456
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 1 cut(s) 1037
MalI GATC 3 cut(s) 174, 369, 938
MboI GATC 3 cut(s) 172, 367, 936
MhlI GDGCHC 1 cut(s) 825
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 50, 270, 468, 674, 778, 855
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 728, 866
MmeI TCCRAC 4 cut(s) 234, 540, 597, 1043
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 300, 806
MroI TCCGGA 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 1138
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 423, 812
MslI CAYNNNNRTG 1 cut(s) 1057
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 659
Mva1269I GAATGC 3 cut(s) 107, 596, 806
MvnI CGCG 1 cut(s) 766
MwoI GCNNNNNNNGC 2 cut(s) 304, 418
NdeII GATC 3 cut(s) 172, 367, 936
NlaIII CATG 4 cut(s) 295, 437, 638, 688
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 1037
NsiI ATGCAT 2 cut(s) 300, 806
OliI CACNNNNGTG 1 cut(s) 1057
PaeR7I CTCGAG 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 1144
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PctI GAATGC 3 cut(s) 107, 596, 806
PdmI GAANNNNTTC 1 cut(s) 1138
PfeI GAWTC 3 cut(s) 220, 962, 1075
PflMI CCANNNNNTGG 1 cut(s) 195
PkrI GCNGC 2 cut(s) 235, 306
PleI GAGTC 2 cut(s) 728, 866
PpsI GAGTC 2 cut(s) 728, 866
PspN4I GGNNCC 1 cut(s) 339
PspXI VCTCGAGB 1 cut(s) 21
RsaI GTAC 3 cut(s) 290, 655, 850
RsaNI GTAC 3 cut(s) 289, 654, 849
RseI CAYNNNNRTG 1 cut(s) 1057
SapI GCTCTTC 1 cut(s) 1144
SaqAI TTAA 2 cut(s) 423, 812
SatI GCNGC 2 cut(s) 234, 305
Sau3AI GATC 3 cut(s) 172, 367, 936
ScaI AGTACT 1 cut(s) 655
SchI GAGTC 2 cut(s) 728, 866
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 3 cut(s) 285, 830, 1009
Sfr274I CTCGAG 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 1057
SmlI CTYRAG 3 cut(s) 21, 645, 926
SmoI CTYRAG 3 cut(s) 21, 645, 926
Sse9I AATT 6 cut(s) 50, 270, 468, 674, 778, 855
SsiI CCGC 2 cut(s) 766, 1016
SspMI CTAG 3 cut(s) 87, 447, 456
StyI CCWWGG 1 cut(s) 917
TaaI ACNGT 2 cut(s) 418, 848
TaiI ACGT 1 cut(s) 501
TaqI TCGA 2 cut(s) 22, 35
TasI AATT 6 cut(s) 50, 270, 468, 674, 778, 855
TatI WGTACW 2 cut(s) 288, 653
TfiI GAWTC 3 cut(s) 220, 962, 1075
Tru1I TTAA 2 cut(s) 423, 812
Tru9I TTAA 2 cut(s) 423, 812
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 1037
TseI GCWGC 2 cut(s) 233, 304
Tsp45I GTSAC 1 cut(s) 1037
TspDTI ATGAA 4 cut(s) 480, 651, 687, 708
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 195
XapI RAATTY 2 cut(s) 50, 674
XhoI CTCGAG 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 1138
XspI CTAG 3 cut(s) 87, 447, 456
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 2 cut(s) 300, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.