FvH4_3g14670

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004982
Physical Location & Seq
Forward (+)
9043323 .. 9050915
7593 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14670.t3

Sequence Viewer

Length: 3666 bp
ATGGAACCTGGAAGGGAGGAATTTCATCCTTCATCCCAGCAATATATGCCAAACTCTCTGAGCAGTATGCATTCTGATATGAGGTCTCATAACCTTAATACTTCAGAAATTAAACCTGTACATAATTATTCCATAACAGGTGAGGAGTTTTCTCTTGAATTTATGCTTGATCGAGTGAACCCCAGGAAGCCTTTGCACCCAAATGCTGTGGGTGATCCAAGTTATGTTACTGACTATGTTGAACTAAAAGGAATGTTAGGAATCAGAGGTTTTGAAAGTGGTTCAGATGCTTCAATGGTTGCTATACCTGAAAGAGGTCCAAACCAGTTTGAGAGAAACAGCTCATCTTTACATGATGGCAGAAATAACTATGGATCAGTTCAATCAGTACCGCGAGTTTCATCAGGCTATGGAAGTAGTCGTGTTCATGGATATACCTTATCTGGAGCCTCTGATAGCACATCAATGTTAATGAAGGTTCTTTGTAGCTTTGGTGGTAAAATTTTGCCCCGTCCAAGTGATGGGAAGCTCAGGTATGTTGGAGGTGATACACGCATTATCCGTATTAGAAAGGACATTACCTGGCAGGAGCTTATACATAAAGCCTTATCAATTTATAACCAATTGCATGTAATTAAGTATCAGCTTCCAGGAGAGGAGCTTGATGCCTTGGTTTCTGTATCTTGTGATGAGGATCTGCAGAATATGATGGAGGAATGTAATGAAATAGAAGATAAAGAGGGGCCGCACAAGCTTAGAATGTTCTTTTTCTCCATCAGTGATTTGGAGGATCATTTTGGTCTGCACACTGTGGATGGCGACTCTGAGGTTCAGTATGTTGTTGCTGTCAATGGCATGGACCTGGGATCAAGAAAAAGCTCGACCATACATGGGTTGACAAGCTCGGTAGCTAATCAATTAGATGAGACGAATAGACTCAATATTCAGAAGGGAACAAGTAGTGTTGTGAAAGACTCAATAGGGTTAGGTGCTCCAGTTTTGACTGGAAAAATCATTTCAGCAACAGCTGCTCAATCTTCTGAACCTATTCTGCCAAGCTCTTCACATGCCTATGAAGCTTATCCTCATTTTCAACATGGACACGTTATGCATTATGGACAAAATGTGCAGGACCCATTGCAGAATGGCCATGCCTTTCCAAGTCAATCCCATTTTGGAGACACTCCTACTTCAGTGCCACATCATGGGATTCATGGTATAATGAATGGAGGAGGAGGTTCAATTGAGGGGCAGACCAGTGGCTCGAGAGAGCGTAACTTTGAGATGCCAATGAAGGAGGTACAGCCATTACATGATGGTTCCTTTCAGCAAGAGAGTGACCCTGAAAAACTTCGCCCCTCAAGGAAAGAGCAATCTGTCCCCAAACCGCTGTATGATGGTAATTTGATGAACTATCCTCCTGTTGAAGAAGCATCAAAAGATGAAAGGAAGTACCAGGAACCTGAAAATGTTGCTTCATCAATTGATTCCGGGATGCTGGTTCACAATCCTAGTGAAGTGGATCATCTGTCCACATCTAACAATGCATTTGCTCCTACCTATGCTGAATCAATGTCCAATGAGATTGATTTGGGGTACCTTGAGCCACCTGTGCAGCCTCAGAGAATTTACTACTCGGAAAGAATTCCCAGGGAGCAAGCAGAGTTGCTGAATCGATCGTCGAAATCTGATGATTCGCATGGTCCTCAGTTTCTCGTATCTCATTCACGCTCTGATATCACCCACCAGGATCCAATTACAGGAGTTAAGAAATTGCATGATCATGCAAATCTTCCTCCCCGGACTGAACAACAATCCTCCTCAACTGTATATGTGGATGCTCAATCTGTTGATGATGGACTTGCCCAACTTCAGAAGTACAAAGAGTTTGCTGATTCAATTTGTGAGATGAATGCTAAGCTTTTGCAAGATGCAGATGGTGAATTAAAGCCAGCATTGCTAAACCCTGCGGATACTAAAGAGTTCACAAACAGGGATAGGATCCTTAAATCTGACCAAGATGCAAACTGCCTGAAAGGTAGCCATAAGAAACTTGTCACTGACGACATTGCAGAAGCTGTATCAGATTGTCCAACTGTAAGTCAGATACCTTCTATGAAGCACCATGAGGTTCCTGCATCTAATCATTCAGAGCTTAATCAGGACGAGTCTACTGGCAAGGATCCAAATACTGCTGACAATATGGGCCATGCACAAGTTGGTACATCTACTCCAGTGCAGGGAGACATAATTATTGATATCGAAGAACGATTTCCTCGTGATTTCCTGTCTGATATATTCTCCAAGGCAATACTTTCTGAAGGCTCCCCTGATGTTGGTTTACTGCAGAAGGATGGAGTTGGTTTGAGCTTTAAAATGGAAAATCATGATCCTAAGCGGTGGTCATATTTCCAAAAGTTGGCTCAAGAAGGGGCTGATCAACAAGATGTTTCTCTTATGGACCAGGATCTTGGTTTTCCTTCTGCAATTAGAAATGTTGAGGAAGACGATAGTAAATCGTATCATCGTACGCCTTTACCAACAGATGGAGTTCCAATGGCCCATATGAATTCTCAGCCTAATTTTGCAGAAGATATTAGCAGGGAAACTGGATTGCCTAAAGCTAATTATGATCATCAGCAACTGAAGGAAACTGAAAGTATGCAGTTTGAGGCCATGATGGAAAACCTAAGAGTTCCACAGTCTGATTATGAGCAAGGAAAGTCTACAAGTAGAACCGCTGGTTTACCTCCATTAAATCCTTCACTGGGAGAGTTTGACATCAGTACCTTGCAGTTGATAAAGAATGAAGATCTAGAACAGATGAAGGAATTGGGTTCTGGCACCTTTGGGACTGTGTATCATGGAAAATGGAGGGGGAGTGATGTTGCCATTAAAAGGCTAAATAAGAGCTGCTTCACTGGGAGATCATCAGAGCAAGAGAGACTGTCTGTTGAGTTCTGGCGGGAAGCTGACATTCTTTCAAAGCTTCACCATCCAAATGTGGTGGCATTTTATGGTGTAGTGCAAGATGGGCCAGGGGGAACACTAGCCACTGTAACTGAGTACATGGTTGATGGTTCTCTTAGGCATGTCTTGCTTCGAAAAGATAGGTATCTTGATCGTCGCAAGCGATTAATAATTGCCATGGATGCAGCATTTGGAATGGAATATTTGCACTCAAAGAACATTGTGCATTTTGACCTGAAGTGTGACAACTTGCTTGTGAACCTAAAAGATCCTCAACGGCCAATTTGCAAGGTCGGTGACTTTGGGCTTTCAAAAATCAAGCGAAATACGCTGGTTTCTGGTGGTGTGCGGGGAACATTACCATGGATGGCCCCAGAGTTGCTGAATGGAAGCAGCACTAAGGTCTCCGAAAAGGTGGATATTTTCTCCTTTGGTATTGTCTTGTGGGAGATTCTTACCGGAGAGGAACCATATGCCAACATGCACTATGGAGCGATTATAGGCGGTATTGTGAACAACACATTGAGACCCACCATTCCAAGTTACTGTGATCCAGAGTGGAGGACGCTTATGGAGCAGTGTTGGGCTCCTAATCCTGCTGCGAGGCCATCATTCACAGAAATTGCTAGTTGCTTACGAGTTATGACTAGAGCCGCTAGCCAAACCAAAGCACAGGGTCACAAGGCATCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1222

Amino Acids

135.58

Weight (kDa)

5.47

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 174 - 255 2.1e-18 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
Acc65I GGTACC 1 cut(s) 1596
AccB1I GGYRCC 2 cut(s) 1596, 2843
AccB7I CCANNNNNTGG 4 cut(s) 521, 1205, 2419, 2546
AccI GTMKAC 2 cut(s) 2171, 2726
AccII CGCG 1 cut(s) 394
AcoI YGGCCR 2 cut(s) 1147, 3248
AcsI RAATTY 6 cut(s) 20, 158, 501, 1626, 1644, 2569
AcuI CTGAAG 6 cut(s) 87, 1176, 1856, 2340, 2666, 3227
AdeI CACNNNGTG 1 cut(s) 811
AflIII ACRYGT 1 cut(s) 1102
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 7 cut(s) 90, 920, 1173, 2238, 2938, 3379, 3490
AlwNI CAGNNNCTG 2 cut(s) 2078, 2644
Ama87I CYCGRG 1 cut(s) 1264
AoxI GGCC 9 cut(s) 743, 1147, 2206, 2559, 2673, 3035, 3248, 3339, 3575
ApeKI GCWGC 6 cut(s) 1028, 1615, 2913, 3155, 3363, 3569
ApoI RAATTY 6 cut(s) 20, 158, 501, 1626, 1644, 2569
AseI ATTAAT 1 cut(s) 3137
Asp700I GAANNNNTTC 4 cut(s) 1047, 1350, 1644, 2271
Asp718I GGTACC 1 cut(s) 1596
AsuC2I CCSGG 2 cut(s) 1492, 1801
AsuHPI GGTGA 7 cut(s) 152, 224, 557, 1732, 1952, 2984, 3278
AsuII TTCGAA 1 cut(s) 3103
AsuNHI GCTAGC 1 cut(s) 3626
AvaI CYCGRG 1 cut(s) 1264
AvaII GGWCC 5 cut(s) 317, 859, 1132, 1703, 2461
BaeI ACNNNNGTAYC 2 cut(s) 2770, 2803
BalI TGGCCA 1 cut(s) 1149
BamHI GGATCC 3 cut(s) 1750, 2001, 2182
BanI GGYRCC 2 cut(s) 1596, 2843
BanII GRGCYC 1 cut(s) 3559
BauI CACGAG 1 cut(s) 2277
BbsI GAAGAC 1 cut(s) 2511
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 6 cut(s) 1015, 1627, 2900, 3167, 3375, 3556
BceAI ACGGC 1 cut(s) 3263
BciVI GTATCC 1 cut(s) 1966
BclI TGATCA 3 cut(s) 1780, 2437, 2632
BcnI CCSGG 2 cut(s) 1492, 1801
BcoDI GTCTC 7 cut(s) 90, 920, 1173, 2238, 2938, 3379, 3490
BfaI CTAG 6 cut(s) 1512, 2816, 3050, 3597, 3618, 3627
BfmI CTRYAG 2 cut(s) 698, 2345
BfuI GTATCC 1 cut(s) 1966
BglII AGATCT 1 cut(s) 2812
BisI GCNGC 8 cut(s) 746, 1029, 1616, 2914, 3156, 3364, 3570, 3624
BlpI GCTNAGC 1 cut(s) 1917
BlsI GCNGC 8 cut(s) 747, 1030, 1617, 2915, 3157, 3365, 3571, 3625
Bme18I GGWCC 5 cut(s) 317, 859, 1132, 1703, 2461
BmeT110I CYCGRG 1 cut(s) 1264
BmrI ACTGGG 2 cut(s) 2777, 2931
BmtI GCTAGC 1 cut(s) 3630
BmuI ACTGGG 2 cut(s) 2777, 2931
BpiI GAAGAC 1 cut(s) 2511
BpmI CTGGAG 3 cut(s) 465, 978, 2217
Bpu10I CCTNAGC 2 cut(s) 530, 2394
Bpu1102I GCTNAGC 1 cut(s) 1917
Bpu14I TTCGAA 1 cut(s) 3103
BpuEI CTTGAG 3 cut(s) 1345, 1622, 2409
BpuMI CCSGG 2 cut(s) 1492, 1801
Bsa29I ATCGAT 1 cut(s) 1675
BsaBI GATNNNNATC 2 cut(s) 693, 3114
BsaI GGTCTC 3 cut(s) 90, 3379, 3490
BsaWI WCCGGW 1 cut(s) 3428
BsaXI ACNNNNNCTCC 4 cut(s) 2215, 2245, 2866, 2896
Bse1I ACTGG 9 cut(s) 325, 995, 1009, 1257, 2179, 2234, 2614, 2772, 2926
Bse3DI GCAATG 3 cut(s) 1136, 1955, 2067
Bse8I GATNNNNATC 2 cut(s) 693, 3114
BseCI ATCGAT 1 cut(s) 1675
BseGI GGATG 9 cut(s) 25, 32, 820, 1500, 1843, 2359, 2995, 3157, 3342
BseJI GATNNNNATC 2 cut(s) 693, 3114
BseMI GCAATG 3 cut(s) 1136, 1955, 2067
BseMII CTCAG 7 cut(s) 50, 544, 816, 1634, 1721, 2588, 3054
BseNI ACTGG 9 cut(s) 325, 995, 1009, 1257, 2179, 2234, 2614, 2772, 2926
BseRI GAGGAG 5 cut(s) 158, 671, 1245, 1248, 1810
BseXI GCAGC 6 cut(s) 1015, 1627, 2900, 3167, 3375, 3556
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 4 cut(s) 788, 1148, 1634, 2258
Bsh1236I CGCG 1 cut(s) 394
Bsh1285I CGRYCG 1 cut(s) 1679
BshFI GGCC 9 cut(s) 745, 1149, 2208, 2561, 2675, 3037, 3250, 3341, 3577
BshNI GGYRCC 2 cut(s) 1596, 2843
BshVI ATCGAT 1 cut(s) 1675
BsiEI CGRYCG 1 cut(s) 1679
BsiHKAI GWGCWC 1 cut(s) 994
BsiHKCI CYCGRG 1 cut(s) 1264
BsiSI CCGG 3 cut(s) 1491, 1801, 3429
BsiWI CGTACG 1 cut(s) 2528
BslFI GGGAC 2 cut(s) 1364, 2866
BsmAI GTCTC 7 cut(s) 90, 920, 1173, 2238, 2938, 3379, 3490
BsmBI CGTCTC 1 cut(s) 920
BsmFI GGGAC 2 cut(s) 1364, 2866
BsmI GAATGC 2 cut(s) 70, 1918
BsnI GGCC 9 cut(s) 745, 1149, 2208, 2561, 2675, 3037, 3250, 3341, 3577
Bso31I GGTCTC 3 cut(s) 90, 3379, 3490
BsoBI CYCGRG 1 cut(s) 1264
Bsp119I TTCGAA 1 cut(s) 3103
Bsp1286I GDGCHC 2 cut(s) 994, 3559
Bsp1407I TGTACA 1 cut(s) 118
Bsp1720I GCTNAGC 1 cut(s) 1917
Bsp19I CCATGG 2 cut(s) 3147, 3332
BspANI GGCC 9 cut(s) 745, 1149, 2208, 2561, 2675, 3037, 3250, 3341, 3577
BspCNI CTCAG 7 cut(s) 51, 543, 817, 1633, 1720, 2587, 3055
BspDI ATCGAT 1 cut(s) 1675
BspFNI CGCG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 2386
BspMAI CTGCAG 2 cut(s) 702, 2349
BspOI GCTAGC 1 cut(s) 3630
BspQI GCTCTTC 1 cut(s) 1066
BspT104I TTCGAA 1 cut(s) 3103
BspT107I GGYRCC 2 cut(s) 1596, 2843
BspTNI GGTCTC 3 cut(s) 90, 3379, 3490
BsrDI GCAATG 3 cut(s) 1136, 1955, 2067
BsrGI TGTACA 1 cut(s) 118
BsrI ACTGG 9 cut(s) 325, 995, 1009, 1257, 2179, 2234, 2614, 2772, 2926
BssSI CACGAG 1 cut(s) 2277
BssT1I CCWWGG 4 cut(s) 669, 2304, 3147, 3332
Bst2BI CACGAG 1 cut(s) 2277
Bst4CI ACNGT 8 cut(s) 811, 1828, 2098, 2703, 2857, 2949, 3058, 3518
Bst6I CTCTTC 1 cut(s) 1066
BstAPI GCANNNNNTGC 3 cut(s) 46, 1028, 3097
BstAUI TGTACA 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 3103
BstC8I GCNNGC 4 cut(s) 1659, 1953, 3131, 3628
BstDSI CCRYGG 2 cut(s) 3147, 3332
BstENI CCTNNNNNAGG 1 cut(s) 312
BstF5I GGATG 9 cut(s) 25, 32, 820, 1500, 1843, 2359, 2995, 3157, 3342
BstFNI CGCG 1 cut(s) 394
BstMAI GTCTC 7 cut(s) 90, 920, 1173, 2238, 2938, 3379, 3490
BstMCI CGRYCG 1 cut(s) 1679
BstNSI RCATGY 4 cut(s) 632, 1070, 3095, 3454
BstSFI CTRYAG 2 cut(s) 698, 2345
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 6 cut(s) 1015, 1627, 2900, 3167, 3375, 3556
BstV2I GAAGAC 1 cut(s) 2511
BstX2I RGATCY 7 cut(s) 694, 1750, 2001, 2182, 2467, 2812, 3238
BstXI CCANNNNNNTGG 1 cut(s) 2471
BstYI RGATCY 7 cut(s) 694, 1750, 2001, 2182, 2467, 2812, 3238
Bsu15I ATCGAT 1 cut(s) 1675
BsuI GTATCC 1 cut(s) 1966
BsuRI GGCC 9 cut(s) 745, 1149, 2208, 2561, 2675, 3037, 3250, 3341, 3577
BsuTUI ATCGAT 1 cut(s) 1675
BtgI CCRYGG 2 cut(s) 3147, 3332
BtsCI GGATG 9 cut(s) 25, 32, 820, 1500, 1843, 2359, 2995, 3157, 3342
BtsI GCAGTG 1 cut(s) 3554
Cac8I GCNNGC 4 cut(s) 1659, 1953, 3131, 3628
CaiI CAGNNNCTG 2 cut(s) 2078, 2644
CciI TCATGA 1 cut(s) 2386
ClaI ATCGAT 1 cut(s) 1675
CseI GACGC 1 cut(s) 3544
CspCI CAANNNNNGTGG 4 cut(s) 189, 224, 2988, 3023
DraI TTTAAA 1 cut(s) 2374
DraIII CACNNNGTG 1 cut(s) 811
EaeI YGGCCR 2 cut(s) 1147, 3248
Eam1104I CTCTTC 1 cut(s) 1066
EarI CTCTTC 1 cut(s) 1066
Eco130I CCWWGG 4 cut(s) 669, 2304, 3147, 3332
Eco24I GRGCYC 1 cut(s) 3559
Eco31I GGTCTC 3 cut(s) 90, 3379, 3490
Eco32I GATATC 2 cut(s) 1738, 2260
Eco47I GGWCC 5 cut(s) 317, 859, 1132, 1703, 2461
Eco57I CTGAAG 6 cut(s) 87, 1176, 1856, 2340, 2666, 3227
Eco88I CYCGRG 1 cut(s) 1264
EcoNI CCTNNNNNAGG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 1132
EcoRI GAATTC 2 cut(s) 1644, 2569
EcoRV GATATC 2 cut(s) 1738, 2260
EcoT14I CCWWGG 4 cut(s) 669, 2304, 3147, 3332
EcoT22I ATGCAT 3 cut(s) 72, 1113, 1549
EcoT38I GRGCYC 1 cut(s) 3559
ErhI CCWWGG 4 cut(s) 669, 2304, 3147, 3332
Esp3I CGTCTC 1 cut(s) 920
FalI AAGNNNNNCTT 2 cut(s) 2900, 2932
FaqI GGGAC 2 cut(s) 1364, 2866
FauI CCCGC 2 cut(s) 2958, 3312
FauNDI CATATG 2 cut(s) 2565, 3442
FbaI TGATCA 3 cut(s) 1780, 2437, 2632
FblI GTMKAC 2 cut(s) 2171, 2726
Fnu4HI GCNGC 8 cut(s) 746, 1029, 1616, 2914, 3156, 3364, 3570, 3624
FokI GGATG 9 cut(s) 12, 19, 827, 1507, 1850, 2366, 2982, 3164, 3349
FriOI GRGCYC 1 cut(s) 3559
Fsp4HI GCNGC 8 cut(s) 746, 1029, 1616, 2914, 3156, 3364, 3570, 3624
FspBI CTAG 6 cut(s) 1512, 2816, 3050, 3597, 3618, 3627
GluI GCNGC 8 cut(s) 746, 1029, 1616, 2914, 3156, 3364, 3570, 3624
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 3 cut(s) 465, 978, 2217
HaeIII GGCC 9 cut(s) 745, 1149, 2208, 2561, 2675, 3037, 3250, 3341, 3577
HapII CCGG 3 cut(s) 1491, 1801, 3429
HgaI GACGC 1 cut(s) 3544
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HindIII AAGCTT 4 cut(s) 752, 1077, 1919, 2987
HpaII CCGG 3 cut(s) 1491, 1801, 3429
HphI GGTGA 7 cut(s) 152, 224, 557, 1732, 1952, 2984, 3278
Hpy99I CGWCG 2 cut(s) 1684, 3129
HpyCH4III ACNGT 8 cut(s) 811, 1828, 2098, 2703, 2857, 2949, 3058, 3518
HpyCH4IV ACGT 1 cut(s) 1104
HpySE526I ACGT 1 cut(s) 1104
KpnI GGTACC 1 cut(s) 1600
Ksp22I TGATCA 3 cut(s) 1780, 2437, 2632
LguI GCTCTTC 1 cut(s) 1066
Lsp1109I GCAGC 6 cut(s) 1015, 1627, 2900, 3167, 3375, 3556
MaeI CTAG 6 cut(s) 1512, 2816, 3050, 3597, 3618, 3627
MaeII ACGT 1 cut(s) 1104
MaeIII GTNAC 9 cut(s) 226, 1274, 1337, 2056, 3058, 3212, 3266, 3512, 3647
MboII GAAGA 9 cut(s) 743, 1029, 1053, 1439, 1784, 2276, 2516, 2603, 2822
MfeI CAATTG 3 cut(s) 623, 1242, 1482
MflI RGATCY 7 cut(s) 694, 1750, 2001, 2182, 2467, 2812, 3238
MhlI GDGCHC 2 cut(s) 994, 3559
MlsI TGGCCA 1 cut(s) 1149
MluNI TGGCCA 1 cut(s) 1149
MlyI GAGTC 4 cut(s) 815, 930, 968, 2177
MmeI TCCRAC 2 cut(s) 520, 2117
Mox20I TGGCCA 1 cut(s) 1149
Mph1103I ATGCAT 3 cut(s) 72, 1113, 1549
MroXI GAANNNNTTC 4 cut(s) 1047, 1350, 1644, 2271
MscI TGGCCA 1 cut(s) 1149
MslI CAYNNNNRTG 7 cut(s) 201, 464, 1071, 1782, 3000, 3331, 3661
Msp20I TGGCCA 1 cut(s) 1149
MspA1I CMGCKG 3 cut(s) 1028, 1390, 2741
MspI CCGG 3 cut(s) 1491, 1801, 3429
MunI CAATTG 3 cut(s) 623, 1242, 1482
Mva1269I GAATGC 2 cut(s) 70, 1918
MvnI CGCG 1 cut(s) 394
NciI CCSGG 2 cut(s) 1492, 1801
NcoI CCATGG 2 cut(s) 3147, 3332
NdeI CATATG 2 cut(s) 2565, 3442
NheI GCTAGC 1 cut(s) 3626
NmuCI GTSAC 5 cut(s) 1337, 2056, 3212, 3266, 3647
NsiI ATGCAT 3 cut(s) 72, 1113, 1549
NspI RCATGY 4 cut(s) 632, 1070, 3095, 3454
NspV TTCGAA 1 cut(s) 3103
PaeR7I CTCGAG 1 cut(s) 1264
PagI TCATGA 1 cut(s) 2386
PasI CCCWGGG 1 cut(s) 1650
PciSI GCTCTTC 1 cut(s) 1066
PcsI WCGNNNNNNNCGW 3 cut(s) 559, 2275, 3130
PctI GAATGC 2 cut(s) 70, 1918
PdmI GAANNNNTTC 4 cut(s) 1047, 1350, 1644, 2271
PfeI GAWTC 8 cut(s) 261, 1210, 1487, 1568, 1672, 1694, 1895, 3421
Pfl23II CGTACG 1 cut(s) 2528
PflMI CCANNNNNTGG 4 cut(s) 521, 1205, 2419, 2546
PfoI TCCNGGA 2 cut(s) 649, 1490
PkrI GCNGC 8 cut(s) 747, 1030, 1617, 2915, 3157, 3365, 3571, 3625
Ple19I CGATCG 1 cut(s) 1679
PleI GAGTC 4 cut(s) 815, 930, 968, 2176
PpsI GAGTC 4 cut(s) 815, 930, 968, 2176
PpuMI RGGWCCY 1 cut(s) 1132
PshBI ATTAAT 1 cut(s) 3137
PsiI TTATAA 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 1132
PspFI CCCAGC 1 cut(s) 36
PspLI CGTACG 1 cut(s) 2528
PspPPI RGGWCCY 1 cut(s) 1132
PstI CTGCAG 2 cut(s) 702, 2349
PstNI CAGNNNCTG 2 cut(s) 2078, 2644
PsuI RGATCY 7 cut(s) 694, 1750, 2001, 2182, 2467, 2812, 3238
PvuI CGATCG 1 cut(s) 1679
PvuII CAGCTG 1 cut(s) 1028
RseI CAYNNNNRTG 7 cut(s) 201, 464, 1071, 1782, 3000, 3331, 3661
SapI GCTCTTC 1 cut(s) 1066
SatI GCNGC 8 cut(s) 746, 1029, 1616, 2914, 3156, 3364, 3570, 3624
SchI GAGTC 4 cut(s) 815, 930, 968, 2177
SduI GDGCHC 2 cut(s) 994, 3559
SfcI CTRYAG 2 cut(s) 698, 2345
Sfr274I CTCGAG 1 cut(s) 1264
SfuI TTCGAA 1 cut(s) 3103
SinI GGWCC 5 cut(s) 317, 859, 1132, 1703, 2461
SlaI CTCGAG 1 cut(s) 1264
SmiMI CAYNNNNRTG 7 cut(s) 201, 464, 1071, 1782, 3000, 3331, 3661
SmlI CTYRAG 4 cut(s) 1264, 1360, 1601, 2424
SmoI CTYRAG 4 cut(s) 1264, 1360, 1601, 2424
SspI AATATT 2 cut(s) 943, 3173
SspMI CTAG 6 cut(s) 1512, 2816, 3050, 3597, 3618, 3627
StyI CCWWGG 4 cut(s) 669, 2304, 3147, 3332
TaaI ACNGT 8 cut(s) 811, 1828, 2098, 2703, 2857, 2949, 3058, 3518
TaiI ACGT 1 cut(s) 1107
TaqI TCGA 7 cut(s) 172, 881, 1265, 1675, 1682, 2262, 3103
TatI WGTACW 3 cut(s) 118, 1878, 3066
TauI GCSGC 2 cut(s) 748, 3626
TfiI GAWTC 8 cut(s) 261, 1210, 1487, 1568, 1672, 1694, 1895, 3421
TseFI GTSAC 5 cut(s) 1337, 2056, 3212, 3266, 3647
TseI GCWGC 6 cut(s) 1028, 1615, 2913, 3155, 3363, 3569
Tsp45I GTSAC 5 cut(s) 1337, 2056, 3212, 3266, 3647
TspGWI ACGGA 1 cut(s) 551
Van91I CCANNNNNTGG 4 cut(s) 521, 1205, 2419, 2546
VpaK11BI GGWCC 5 cut(s) 317, 859, 1132, 1703, 2461
VspI ATTAAT 1 cut(s) 3137
XagI CCTNNNNNAGG 1 cut(s) 312
XapI RAATTY 6 cut(s) 20, 158, 501, 1626, 1644, 2569
XbaI TCTAGA 1 cut(s) 2815
XceI RCATGY 4 cut(s) 632, 1070, 3095, 3454
XcmI CCANNNNNNNNNTGG 2 cut(s) 781, 2216
XhoI CTCGAG 1 cut(s) 1264
XmiI GTMKAC 2 cut(s) 2171, 2726
XmnI GAANNNNTTC 4 cut(s) 1047, 1350, 1644, 2271
XspI CTAG 6 cut(s) 1512, 2816, 3050, 3597, 3618, 3627
Zsp2I ATGCAT 3 cut(s) 72, 1113, 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.