Rmu_sc0004033.1_g000011

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004033.1
OG0004982
Physical Location & Seq
Reverse (-)
30582 .. 36128
5547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004033.1_g000011.1.cds

Sequence Viewer

Length: 3657 bp
atggaacctggaagggaggaattccaccctgcatcccagcaatatatgccgaactctctgagcagcatgcattctgatatgaggtcccataaccttaatacttcagatattaaacctgtacataattattctataacaggtgaggagttttctcttgaatttatgcttgatcgagtgaaccccaggaagcctttgcatccaaatgctgtggctgatccaagttatgttgaactaaaaggaatgttaggaatcagtcacggaggttttgaaagtggttcagatgcttcaatggttgctatatctgaaagaggtccaaaccagtttgagagaaacagctcatatttacacgatgacagaaataactatggatcagttcaatcggtaccacgagcttcttcaggctatggaaatagtcgtgttcatggatatgcctcatctggagcctctgatagctcatcaatgagaatgaaggttctttgtagctttggtggtaaaattttaccccgaccaagtgatgggaaactcaggtatgttggaggtgatacacgcattatccgtattagaaaggacattacctggcaggagcttatacataaagccttatcaatttataaccaattgcatataattaaatatcagcttccaggagaggagcttgatgctttggtttctgtatcttgtgatgaggatctgcagaatatgatggaggaatggaatgaactagaagataaagaggggccgcagaagcttagaatgttttttttctccttcagtgatttggaggatcagtttggtctgcacactgtggatggtgactctgaggttcagtatgtagttgctgtcaatggcatggacctgggatcaagaaaaaactcgagcctacatggtttgacaagctcggcagctaatcatttagatgacacaaatagacacaatattcagaagggaacaagtagtgttgcgaaagactcaatagggataggtgctccagttttgactggcaaaatcatttcagcgacaactgttcaatcttctgaacctatgctgccaagctcttcaagtgcatatgaagcttatcctcatttccaacatgggcaggttatgcattatggacaaaatgtgcagtacccattgcacaatggccatgcctttccaagtcaatcccattttggagacgctcctatttcagtgcctcatcatggtattatgaatcaaggaggaggttcaattgaagggctgaccagtggcttgagagagcatagtattgagatgccagtgaaggaggtacaaccattacgtgatggttcagtccagcaagagagtgaccctgaaaaacttcgcccctcaaggaaagagcaatctgtccccaaaccgctgtatgatggtaatttgatgaactaccctcctgttgaagaagcatcaaaagatgaaagaaagtaccaggaacctgaaaatgttgcttcatcaattgattccgggaagtctatgctggttcacaatcatagtgaagttgatcatcagtccacatctaacaatgcatttgctcctacttatgttgacccaatgtccaatggaattgatttggggtaccttgagccacctgtgcagcctcagagaatctactactcggaaagaattcccagggagcaggctgagttgctgaatcgatcgtcaaagtctgatgattcgcatggtgctcagtttctcgtatctcattcacgctctgatatcattcaccaggatccaattacaggagttaagaaattgcatgatcatgcaaaccttcctcccaggtctgaacaacaatcctcctcaactgtaaatgtggatgctcaaactgttgatgatggacttgctcaactgcagaagtacaaagagtttgctgattcaatttgtgagatgaatgctaagcttttgcaagatgcagatggtgaattaaagccagcattggcaaactctgtggatactaaagagatcgcaaacatggataagatccttaaatctgaccaagaaacaaactgcctcaaaggtagccataagaaacttgtggttgatgacattgcagaagctatatcagagtatccggctgtaagtcagataccttctgtgaagcaccatgaggtttctgcatctaatcattcagagcttaatcaggccgaggccactggcaaggatccaaatactgctgacaatatgggccctgcacaagttggtatatctactccagtgcagggagacataattattgatatcgaagaacgatttcctcgtgatttcctgtctgatatattctccaaggcaatactttctgaaggctcccctgatgttggtttactgcagaaggatggagctggtttgagcttaaatgtggaaaatcatgatcctaagcactggtcatatttccaaaagttggctcaagaaggggccgatcaacaagatgtttctcttatggaccaggatcttggttttccttctgcaattagaaatgttgaagaagaagattgtaaatcttatcatcgtacacctttaccaacagatggaattccaatggcccatatgaattctcagcctaattttggagaagatattagcaaagaaactggactgactaaagctaattatgatcatccacaactgaaggaaactgaaagtatgcagtttgaggccatgatggaaaacttacgagtgccagaatcagattatgaggtaggaaagtttgcaagtagagccgctggtttacctcctttaaatccttcactgggagattttgatatcagtaccttgcagttgataaagaatgaagatctagaacagatgaaagaactgggttctggcacctttgggactgtgtatcatggaaaatggagggggagtgatgttgccattaaaagactaaataagagctgcttcactgggagatcatcagagcaagagagactgtctatagagttctggcgggaagctgacattctttcaaagctacaccatccaaatgtggtggcattttatggtgtagtgcaagatgggccagggggaacactggctaccgtaactgagtacatggttgatggttctcttaggcatgtcttacttcgaaaagataggtatcttgatcgtcgcaagcgattaataattgccatggatgcagcatttggaatggaatatttgcactcaaagaatattgttcattttgacctgaagtgtgacaacttacttgtgaacctaaaagatcctcaacggccaatttgcaaggtcggtgattttggcctttcaaagattaagcgaaataccctggtttcgggtggtgtacgtggaacgttaccatggatggctccagagttgctgaatggaagcagcactaaggtctccgaaaaggtggatattttctcctttggtattgtcttatgggagattctcaccggagaggagccgtatgccaacatgcactatggagcaattataggcggtattgtgaacaacacattgagacccaccattccaagttactgtgatccagaatggagaacgcttatggagcagtgttgggctcctaatcctgctgccaggccatcgttcacagaaattgctagttgcttacgagttatgacaacagcagctagccaaaccaaagcacagggtcacaaggcgtctaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1218

Amino Acids

134.83

Weight (kDa)

5.45

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
Acc36I ACCTGC 1 cut(s) 1087
Acc65I GGTACC 2 cut(s) 382, 1587
AccB1I GGYRCC 3 cut(s) 382, 1587, 2834
AccB7I CCANNNNNTGG 3 cut(s) 515, 2410, 2537
AciI CCGC 5 cut(s) 740, 1373, 2730, 2956, 3465
AclI AACGTT 1 cut(s) 3317
AcoI YGGCCR 2 cut(s) 1141, 3239
AcsI RAATTY 6 cut(s) 20, 158, 495, 1635, 2541, 2560
AcuI CTGAAG 6 cut(s) 87, 381, 754, 2331, 2657, 3218
AcyI GRCGYC 1 cut(s) 3647
AdeI CACNNNGTG 1 cut(s) 805
AhdI GACNNNNNGTC 1 cut(s) 1564
Alw21I GWGCWC 2 cut(s) 988, 1699
Alw26I GTCTC 5 cut(s) 1167, 2229, 2929, 3370, 3481
Ama87I CYCGRG 1 cut(s) 874
ApaI GGGCCC 1 cut(s) 2201
ApeKI GCWGC 9 cut(s) 63, 902, 1045, 1606, 2904, 3146, 3354, 3560, 3614
ApoI RAATTY 6 cut(s) 20, 158, 495, 1635, 2541, 2560
AseI ATTAAT 1 cut(s) 3128
Asp700I GAANNNNTTC 3 cut(s) 1335, 1635, 2262
Asp718I GGTACC 2 cut(s) 382, 1587
AsuC2I CCSGG 1 cut(s) 1477
AsuHPI GGTGA 7 cut(s) 152, 551, 824, 1727, 1943, 3269, 3409
AsuII TTCGAA 1 cut(s) 3094
AsuNHI GCTAGC 1 cut(s) 3617
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 4 cut(s) 84, 311, 853, 2452
BaeGI GKGCMC 1 cut(s) 2201
BalI TGGCCA 1 cut(s) 1143
BamHI GGATCC 2 cut(s) 1741, 2173
BanI GGYRCC 3 cut(s) 382, 1587, 2834
BanII GRGCYC 2 cut(s) 2201, 3550
BauI CACGAG 2 cut(s) 387, 2268
Bbv12I GWGCWC 2 cut(s) 988, 1699
BbvI GCAGC 9 cut(s) 75, 914, 1032, 1618, 2891, 3158, 3366, 3547, 3626
BceAI ACGGC 2 cut(s) 3254, 3415
BciVI GTATCC 2 cut(s) 1957, 2091
BclI TGATCA 3 cut(s) 1513, 1771, 2623
BcnI CCSGG 1 cut(s) 1477
BcoDI GTCTC 5 cut(s) 1167, 2229, 2929, 3370, 3481
BfaI CTAG 4 cut(s) 722, 2807, 3588, 3618
BfmI CTRYAG 4 cut(s) 692, 1862, 2336, 2943
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 2 cut(s) 1957, 2091
BglII AGATCT 1 cut(s) 2803
BlpI GCTNAGC 1 cut(s) 1908
Bme18I GGWCC 4 cut(s) 84, 311, 853, 2452
BmeRI GACNNNNNGTC 1 cut(s) 1564
BmeT110I CYCGRG 1 cut(s) 874
BmrI ACTGGG 3 cut(s) 2768, 2834, 2922
BmtI GCTAGC 1 cut(s) 3621
BmuI ACTGGG 3 cut(s) 2768, 2834, 2922
BpmI CTGGAG 4 cut(s) 459, 972, 2208, 3318
Bpu10I CCTNAGC 1 cut(s) 2385
Bpu1102I GCTNAGC 1 cut(s) 1908
Bpu14I TTCGAA 1 cut(s) 3094
BpuEI CTTGAG 4 cut(s) 1270, 1330, 1613, 2400
BpuMI CCSGG 1 cut(s) 1477
Bsa29I ATCGAT 1 cut(s) 1666
BsaAI YACGTR 2 cut(s) 1298, 3311
BsaBI GATNNNNATC 2 cut(s) 687, 3105
BsaHI GRCGYC 1 cut(s) 3647
BsaI GGTCTC 2 cut(s) 3370, 3481
BsaWI WCCGGW 1 cut(s) 3419
BsaXI ACNNNNNCTCC 4 cut(s) 773, 803, 2857, 2887
Bse3DI GCAATG 2 cut(s) 1130, 2058
Bse8I GATNNNNATC 2 cut(s) 687, 3105
BseCI ATCGAT 1 cut(s) 1666
BseGI GGATG 9 cut(s) 32, 196, 814, 1834, 2350, 2626, 2986, 3148, 3333
BseJI GATNNNNATC 2 cut(s) 687, 3105
BseMI GCAATG 2 cut(s) 1130, 2058
BseMII CTCAG 8 cut(s) 50, 538, 810, 1625, 1644, 1712, 2579, 3045
BseRI GAGGAG 5 cut(s) 158, 665, 1233, 1801, 3440
BseSI GKGCMC 1 cut(s) 2201
BseXI GCAGC 9 cut(s) 75, 914, 1032, 1618, 2891, 3158, 3366, 3547, 3626
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 5 cut(s) 782, 1142, 1625, 2187, 2249
Bsh1285I CGRYCG 1 cut(s) 1670
BshNI GGYRCC 3 cut(s) 382, 1587, 2834
BshVI ATCGAT 1 cut(s) 1666
BsiEI CGRYCG 1 cut(s) 1670
BsiHKAI GWGCWC 2 cut(s) 988, 1699
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 3 cut(s) 1476, 2084, 3420
BslFI GGGAC 3 cut(s) 70, 1349, 2857
BsmAI GTCTC 5 cut(s) 1167, 2229, 2929, 3370, 3481
BsmBI CGTCTC 1 cut(s) 1167
BsmFI GGGAC 3 cut(s) 70, 1349, 2857
BsmI GAATGC 2 cut(s) 70, 1909
Bso31I GGTCTC 2 cut(s) 3370, 3481
BsoBI CYCGRG 1 cut(s) 874
Bsp119I TTCGAA 1 cut(s) 3094
Bsp120I GGGCCC 1 cut(s) 2197
Bsp1286I GDGCHC 4 cut(s) 988, 1699, 2201, 3550
Bsp1407I TGTACA 1 cut(s) 118
Bsp1720I GCTNAGC 1 cut(s) 1908
Bsp19I CCATGG 2 cut(s) 3138, 3323
BspACI CCGC 5 cut(s) 740, 1373, 2730, 2956, 3465
BspCNI CTCAG 8 cut(s) 51, 537, 811, 1624, 1645, 1711, 2578, 3046
BspDI ATCGAT 1 cut(s) 1666
BspHI TCATGA 1 cut(s) 2377
BspMAI CTGCAG 3 cut(s) 696, 1866, 2340
BspMI ACCTGC 1 cut(s) 1087
BspOI GCTAGC 1 cut(s) 3621
BspQI GCTCTTC 1 cut(s) 1060
BspT104I TTCGAA 1 cut(s) 3094
BspT107I GGYRCC 3 cut(s) 382, 1587, 2834
BspTNI GGTCTC 2 cut(s) 3370, 3481
BsrDI GCAATG 2 cut(s) 1130, 2058
BsrGI TGTACA 1 cut(s) 118
BssNI GRCGYC 1 cut(s) 3647
BssSI CACGAG 2 cut(s) 387, 2268
BssT1I CCWWGG 3 cut(s) 2295, 3138, 3323
Bst2BI CACGAG 2 cut(s) 387, 2268
Bst4CI ACNGT 8 cut(s) 805, 1024, 1819, 1840, 2848, 2940, 3049, 3509
Bst6I CTCTTC 1 cut(s) 1060
BstACI GRCGYC 1 cut(s) 3647
BstAPI GCANNNNNTGC 2 cut(s) 46, 1102
BstAUI TGTACA 1 cut(s) 118
BstBAI YACGTR 2 cut(s) 1298, 3311
BstBI TTCGAA 1 cut(s) 3094
BstC8I GCNNGC 5 cut(s) 68, 1650, 1944, 3122, 3619
BstDSI CCRYGG 2 cut(s) 3138, 3323
BstF5I GGATG 9 cut(s) 32, 196, 814, 1834, 2350, 2626, 2986, 3148, 3333
BstMAI GTCTC 5 cut(s) 1167, 2229, 2929, 3370, 3481
BstMCI CGRYCG 1 cut(s) 1670
BstMWI GCNNNNNNNGC 9 cut(s) 46, 745, 1070, 1102, 1603, 2726, 3025, 3143, 3535
BstNSI RCATGY 3 cut(s) 70, 3086, 3445
BstSFI CTRYAG 4 cut(s) 692, 1862, 2336, 2943
BstSLI GKGCMC 1 cut(s) 2201
BstV1I GCAGC 9 cut(s) 75, 914, 1032, 1618, 2891, 3158, 3366, 3547, 3626
BstX2I RGATCY 7 cut(s) 688, 1741, 1992, 2173, 2458, 2803, 3229
BstXI CCANNNNNNTGG 1 cut(s) 2462
BstYI RGATCY 7 cut(s) 688, 1741, 1992, 2173, 2458, 2803, 3229
Bsu15I ATCGAT 1 cut(s) 1666
BsuI GTATCC 2 cut(s) 1957, 2091
BsuTUI ATCGAT 1 cut(s) 1666
BtgI CCRYGG 2 cut(s) 3138, 3323
BtsCI GGATG 9 cut(s) 32, 196, 814, 1834, 2350, 2626, 2986, 3148, 3333
BtsI GCAGTG 1 cut(s) 3545
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 5 cut(s) 68, 1650, 1944, 3122, 3619
CciI TCATGA 1 cut(s) 2377
ClaI ATCGAT 1 cut(s) 1666
CseI GACGC 2 cut(s) 1184, 3636
CspCI CAANNNNNGTGG 6 cut(s) 189, 224, 1941, 1976, 2979, 3014
DraI TTTAAA 1 cut(s) 2748
DraIII CACNNNGTG 1 cut(s) 805
DriI GACNNNNNGTC 1 cut(s) 1564
EaeI YGGCCR 2 cut(s) 1141, 3239
Eam1104I CTCTTC 1 cut(s) 1060
Eam1105I GACNNNNNGTC 1 cut(s) 1564
EarI CTCTTC 1 cut(s) 1060
Eco130I CCWWGG 3 cut(s) 2295, 3138, 3323
Eco24I GRGCYC 2 cut(s) 2201, 3550
Eco31I GGTCTC 2 cut(s) 3370, 3481
Eco32I GATATC 3 cut(s) 1729, 2251, 2773
Eco47I GGWCC 4 cut(s) 84, 311, 853, 2452
Eco57I CTGAAG 6 cut(s) 87, 381, 754, 2331, 2657, 3218
Eco88I CYCGRG 1 cut(s) 874
EcoO109I RGGNCCY 2 cut(s) 84, 2198
EcoRI GAATTC 4 cut(s) 20, 1635, 2541, 2560
EcoRV GATATC 3 cut(s) 1729, 2251, 2773
EcoT14I CCWWGG 3 cut(s) 2295, 3138, 3323
EcoT22I ATGCAT 3 cut(s) 72, 1107, 1540
EcoT38I GRGCYC 2 cut(s) 2201, 3550
ErhI CCWWGG 3 cut(s) 2295, 3138, 3323
Esp3I CGTCTC 1 cut(s) 1167
FalI AAGNNNNNCTT 2 cut(s) 2891, 2923
FaqI GGGAC 3 cut(s) 70, 1349, 2857
FauI CCCGC 1 cut(s) 2949
FauNDI CATATG 2 cut(s) 1066, 2556
FbaI TGATCA 3 cut(s) 1513, 1771, 2623
FokI GGATG 9 cut(s) 19, 183, 821, 1841, 2357, 2613, 2973, 3155, 3340
FriOI GRGCYC 2 cut(s) 2201, 3550
FspBI CTAG 4 cut(s) 722, 2807, 3588, 3618
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 4 cut(s) 459, 972, 2208, 3318
HapII CCGG 3 cut(s) 1476, 2084, 3420
HgaI GACGC 2 cut(s) 1184, 3636
Hin1I GRCGYC 1 cut(s) 3647
HincII GTYRAC 1 cut(s) 1558
HindII GTYRAC 1 cut(s) 1558
HindIII AAGCTT 3 cut(s) 746, 1071, 1910
HpaII CCGG 3 cut(s) 1476, 2084, 3420
HphI GGTGA 7 cut(s) 152, 551, 824, 1727, 1943, 3269, 3409
Hpy99I CGWCG 1 cut(s) 3120
HpyCH4III ACNGT 8 cut(s) 805, 1024, 1819, 1840, 2848, 2940, 3049, 3509
HpyCH4IV ACGT 3 cut(s) 1297, 3310, 3317
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 745, 1070, 1102, 1603, 2726, 3025, 3143, 3535
HpySE526I ACGT 3 cut(s) 1297, 3310, 3317
Hsp92I GRCGYC 1 cut(s) 3647
KpnI GGTACC 2 cut(s) 386, 1591
Ksp22I TGATCA 3 cut(s) 1513, 1771, 2623
LguI GCTCTTC 1 cut(s) 1060
Lsp1109I GCAGC 9 cut(s) 75, 914, 1032, 1618, 2891, 3158, 3366, 3547, 3626
MaeI CTAG 4 cut(s) 722, 2807, 3588, 3618
MaeII ACGT 3 cut(s) 1297, 3310, 3317
MaeIII GTNAC 8 cut(s) 254, 812, 1322, 3049, 3203, 3318, 3503, 3638
MfeI CAATTG 3 cut(s) 617, 1227, 1467
MflI RGATCY 7 cut(s) 688, 1741, 1992, 2173, 2458, 2803, 3229
MhlI GDGCHC 4 cut(s) 988, 1699, 2201, 3550
MlsI TGGCCA 1 cut(s) 1143
MluNI TGGCCA 1 cut(s) 1143
MlyI GAGTC 2 cut(s) 809, 962
MmeI TCCRAC 2 cut(s) 514, 1111
Mox20I TGGCCA 1 cut(s) 1143
Mph1103I ATGCAT 3 cut(s) 72, 1107, 1540
MroXI GAANNNNTTC 3 cut(s) 1335, 1635, 2262
MscI TGGCCA 1 cut(s) 1143
MslI CAYNNNNRTG 5 cut(s) 201, 426, 915, 1773, 2991
Msp20I TGGCCA 1 cut(s) 1143
MspA1I CMGCKG 2 cut(s) 1375, 2732
MspI CCGG 3 cut(s) 1476, 2084, 3420
MunI CAATTG 3 cut(s) 617, 1227, 1467
Mva1269I GAATGC 2 cut(s) 70, 1909
MwoI GCNNNNNNNGC 9 cut(s) 46, 745, 1070, 1102, 1603, 2726, 3025, 3143, 3535
NciI CCSGG 1 cut(s) 1477
NcoI CCATGG 2 cut(s) 3138, 3323
NdeI CATATG 2 cut(s) 1066, 2556
NheI GCTAGC 1 cut(s) 3617
NmeAIII GCCGAG 2 cut(s) 878, 2182
NmuCI GTSAC 5 cut(s) 254, 812, 1322, 3203, 3638
NsiI ATGCAT 3 cut(s) 72, 1107, 1540
NspI RCATGY 3 cut(s) 70, 3086, 3445
NspV TTCGAA 1 cut(s) 3094
PaeI GCATGC 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 874
PagI TCATGA 1 cut(s) 2377
PasI CCCWGGG 1 cut(s) 1641
PciSI GCTCTTC 1 cut(s) 1060
PcsI WCGNNNNNNNCGW 3 cut(s) 553, 2266, 3121
PctI GAATGC 2 cut(s) 70, 1909
PdmI GAANNNNTTC 3 cut(s) 1335, 1635, 2262
PfeI GAWTC 9 cut(s) 249, 1210, 1472, 1617, 1663, 1685, 1886, 2693, 3412
PflMI CCANNNNNTGG 3 cut(s) 515, 2410, 2537
PfoI TCCNGGA 2 cut(s) 643, 1475
Ple19I CGATCG 1 cut(s) 1670
PleI GAGTC 2 cut(s) 809, 962
PpsI GAGTC 2 cut(s) 809, 962
Ppu21I YACGTR 2 cut(s) 1298, 3311
PpuMI RGGWCCY 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 3128
PsiI TTATAA 1 cut(s) 612
Psp1406I AACGTT 1 cut(s) 3317
Psp5II RGGWCCY 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 36
PspOMI GGGCCC 1 cut(s) 2197
PspPPI RGGWCCY 1 cut(s) 84
PspXI VCTCGAGB 1 cut(s) 874
PsrI GAACNNNNNNTAC 2 cut(s) 3028, 3060
PstI CTGCAG 3 cut(s) 696, 1866, 2340
PsuI RGATCY 7 cut(s) 688, 1741, 1992, 2173, 2458, 2803, 3229
PvuI CGATCG 1 cut(s) 1670
RseI CAYNNNNRTG 5 cut(s) 201, 426, 915, 1773, 2991
SapI GCTCTTC 1 cut(s) 1060
SchI GAGTC 2 cut(s) 809, 962
SduI GDGCHC 4 cut(s) 988, 1699, 2201, 3550
SfcI CTRYAG 4 cut(s) 692, 1862, 2336, 2943
Sfr274I CTCGAG 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 3094
SinI GGWCC 4 cut(s) 84, 311, 853, 2452
SlaI CTCGAG 1 cut(s) 874
SmiMI CAYNNNNRTG 5 cut(s) 201, 426, 915, 1773, 2991
SmlI CTYRAG 5 cut(s) 874, 1249, 1345, 1592, 2415
SmoI CTYRAG 5 cut(s) 874, 1249, 1345, 1592, 2415
SphI GCATGC 1 cut(s) 70
SsiI CCGC 5 cut(s) 740, 1373, 2730, 2956, 3465
SspI AATATT 3 cut(s) 937, 3164, 3181
SspMI CTAG 4 cut(s) 722, 2807, 3588, 3618
StyI CCWWGG 3 cut(s) 2295, 3138, 3323
TaaI ACNGT 8 cut(s) 805, 1024, 1819, 1840, 2848, 2940, 3049, 3509
TaiI ACGT 3 cut(s) 1300, 3313, 3320
TaqI TCGA 5 cut(s) 172, 875, 1666, 2253, 3094
TatI WGTACW 3 cut(s) 118, 1869, 3057
TauI GCSGC 2 cut(s) 742, 2732
TfiI GAWTC 9 cut(s) 249, 1210, 1472, 1617, 1663, 1685, 1886, 2693, 3412
TseFI GTSAC 5 cut(s) 254, 812, 1322, 3203, 3638
TseI GCWGC 9 cut(s) 63, 902, 1045, 1606, 2904, 3146, 3354, 3560, 3614
Tsp45I GTSAC 5 cut(s) 254, 812, 1322, 3203, 3638
TspGWI ACGGA 2 cut(s) 273, 545
Van91I CCANNNNNTGG 3 cut(s) 515, 2410, 2537
VpaK11BI GGWCC 4 cut(s) 84, 311, 853, 2452
VspI ATTAAT 1 cut(s) 3128
XapI RAATTY 6 cut(s) 20, 158, 495, 1635, 2541, 2560
XbaI TCTAGA 1 cut(s) 2806
XceI RCATGY 3 cut(s) 70, 3086, 3445
XhoI CTCGAG 1 cut(s) 874
XmnI GAANNNNTTC 3 cut(s) 1335, 1635, 2262
XspI CTAG 4 cut(s) 722, 2807, 3588, 3618
Zsp2I ATGCAT 3 cut(s) 72, 1107, 1540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.