Rmu_co8405733.1_g000001

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8405733.1
OG0004982
Physical Location & Seq
Forward (+)
1 .. 1216
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8405733.1_g000001.1.cds

Sequence Viewer

Length: 1140 bp
gagttgctgaatcgatcgtcaaagtctgatgattcgcatggtgctcagtttctcgtatctcattcacgctctgatatcattcaccaggatccaattacaggagttaagaaattgcatgatcatgcaaaccttcctcccaggtctgaacaacaatcctcctcaactgtaaatgtggatgctcaaactgttgatgatggacttgctcaactgcagaagtacaaagagtttgctgattcaatttgtgagatgaatgctaagcttttgcaagatgcagatggtgaattaaagccagcattggcaaactctgtggatactaaagagatcgcaaacatggataagatccttaaatctgaccaagaaacaaactgcctcaaaggtagccataagaaacttgtggttgatgacattgcagaagctatatcagagtatccggctgtaagtcagataccttctgtgaagcaccatgaggtttctgcatctaatcattcagagcttaatcaggccgaggccactggcaaggatccaaatactgctgacaatatgggccctgcacaagttggtatatctactccagtgcagggagacataattattgatatcgaagaacgatttcctcgtgatttcctgtctgatatattctccaaggcaatactttctgaaggctcccctgatgttggtttactgcagaaggatggagctggtttgagcttaaatgtggaaaatcatgatcctaagcactggtcatatttccaaaagttggctcaagaaggggccgatcaacaagatgtttctcttatggaccaggatcttggttttccttctgcaattagaaatgttgaagaagaagattgtaaatcttatcatcgtacacctttaccaacagatggaattccaatggcccatatgaattctcagcctaattttggagaagatattagcaaagaaactggactgactaaagctaattatgatcatccacaactgaaggaaactgaaagtatgcagtttgaggccatgatggaaaacttacgagtgccagaatcagattatgaggtaggaaagtttgcaagtagagccgctggtttacctcctttaaatccttcactgggagattttgatatcagtaccttgcaggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

379

Amino Acids

41.55

Weight (kDa)

4.67

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1123
AccB7I CCANNNNNTGG 2 cut(s) 757, 884
AciI CCGC 1 cut(s) 1077
AclWI GGATC 7 cut(s) 83, 96, 334, 515, 528, 722, 813
AcsI RAATTY 2 cut(s) 888, 907
AcuI CTGAAG 2 cut(s) 678, 1004
AfaI GTAC 3 cut(s) 218, 868, 1126
AfiI CCNNNNNNNGG 7 cut(s) 98, 578, 674, 757, 884, 923, 1106
AgsI TTSAA 2 cut(s) 237, 839
AjnI CCWGG 3 cut(s) 84, 137, 801
AluBI AGCT 6 cut(s) 259, 416, 493, 698, 708, 962
AluI AGCT 6 cut(s) 259, 416, 493, 698, 708, 962
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 1 cut(s) 576
AlwI GGATC 7 cut(s) 83, 96, 334, 515, 528, 722, 813
AoxI GGCC 6 cut(s) 501, 507, 544, 771, 897, 1011
ApaI GGGCCC 1 cut(s) 548
ApoI RAATTY 2 cut(s) 888, 907
Asp700I GAANNNNTTC 1 cut(s) 609
AspS9I GGNCC 5 cut(s) 544, 545, 771, 799, 898
AsuHPI GGTGA 2 cut(s) 74, 290
AvaII GGWCC 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 548
BamHI GGATCC 2 cut(s) 88, 520
BanII GRGCYC 1 cut(s) 548
BauI CACGAG 1 cut(s) 615
Bbv12I GWGCWC 1 cut(s) 46
BccI CCATC 5 cut(s) 188, 269, 686, 878, 1012
BciT130I CCWGG 3 cut(s) 86, 139, 803
BciVI GTATCC 2 cut(s) 304, 438
BclI TGATCA 2 cut(s) 118, 970
BcoDI GTCTC 1 cut(s) 576
BfmI CTRYAG 2 cut(s) 209, 683
BfuAI ACCTGC 1 cut(s) 1123
BfuI GTATCC 2 cut(s) 304, 438
BisI GCNGC 1 cut(s) 1077
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 1078
Bme1390I CCNGG 3 cut(s) 86, 139, 803
Bme18I GGWCC 1 cut(s) 799
BmgT120I GGNCC 5 cut(s) 544, 545, 771, 799, 898
BmiI GGNNCC 5 cut(s) 90, 522, 546, 664, 772
BmrFI CCNGG 3 cut(s) 86, 139, 803
BmrI ACTGGG 1 cut(s) 1115
BmsI GCATC 3 cut(s) 166, 259, 485
BmuI ACTGGG 1 cut(s) 1115
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 732
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 3 cut(s) 137, 504, 642
Bsc4I CCNNNNNNNGG 7 cut(s) 98, 578, 674, 757, 884, 923, 1106
Bse1I ACTGG 5 cut(s) 517, 572, 743, 952, 1110
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 3 cut(s) 86, 139, 803
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 3 cut(s) 137, 504, 642
BseGI GGATG 3 cut(s) 181, 697, 973
BseLI CCNNNNNNNGG 7 cut(s) 98, 578, 674, 757, 884, 923, 1106
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 2 cut(s) 59, 926
BseNI ACTGG 5 cut(s) 517, 572, 743, 952, 1110
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 548
BsgI GTGCAG 2 cut(s) 534, 596
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 6 cut(s) 503, 509, 546, 773, 899, 1013
BshVI ATCGAT 1 cut(s) 13
BsiEI CGRYCG 1 cut(s) 17
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 1 cut(s) 431
BslI CCNNNNNNNGG 7 cut(s) 98, 578, 674, 757, 884, 923, 1106
BsmAI GTCTC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 6 cut(s) 503, 509, 546, 773, 899, 1013
Bsp120I GGGCCC 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 46, 548
Bsp1720I GCTNAGC 1 cut(s) 255
BspACI CCGC 1 cut(s) 1077
BspANI GGCC 6 cut(s) 503, 509, 546, 773, 899, 1013
BspCNI CTCAG 2 cut(s) 58, 925
BspDI ATCGAT 1 cut(s) 13
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 5 cut(s) 90, 522, 546, 664, 772
BspMAI CTGCAG 2 cut(s) 213, 687
BspMI ACCTGC 1 cut(s) 1123
BspPI GGATC 7 cut(s) 83, 96, 334, 515, 528, 722, 813
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 5 cut(s) 517, 572, 743, 952, 1110
BssECI CCNNGG 3 cut(s) 137, 504, 642
BssSI CACGAG 1 cut(s) 615
BssT1I CCWWGG 1 cut(s) 642
Bst2BI CACGAG 1 cut(s) 615
Bst2UI CCWGG 3 cut(s) 86, 139, 803
Bst4CI ACNGT 2 cut(s) 166, 187
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 45, 255, 732, 912
BstF5I GGATG 3 cut(s) 181, 697, 973
BstMAI GTCTC 1 cut(s) 576
BstMCI CGRYCG 1 cut(s) 17
BstMWI GCNNNNNNNGC 1 cut(s) 1073
BstNI CCWGG 3 cut(s) 86, 139, 803
BstSCI CCNGG 3 cut(s) 84, 137, 801
BstSFI CTRYAG 2 cut(s) 209, 683
BstSLI GKGCMC 1 cut(s) 548
BstX2I RGATCY 4 cut(s) 88, 339, 520, 805
BstXI CCANNNNNNTGG 1 cut(s) 809
BstYI RGATCY 4 cut(s) 88, 339, 520, 805
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 2 cut(s) 304, 438
BsuRI GGCC 6 cut(s) 503, 509, 546, 773, 899, 1013
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 3 cut(s) 181, 697, 973
BtsIMutI CAGTG 4 cut(s) 510, 579, 736, 1103
BveI ACCTGC 1 cut(s) 1123
Cac8I GCNNGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 724
Cfr13I GGNCC 5 cut(s) 544, 545, 771, 799, 898
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 3 cut(s) 217, 867, 1125
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviAII CATG 7 cut(s) 38, 116, 122, 331, 464, 725, 1015
CviQI GTAC 3 cut(s) 217, 867, 1125
DdeI CTNAG 4 cut(s) 45, 255, 732, 912
DraI TTTAAA 1 cut(s) 1095
Eco130I CCWWGG 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 548
Eco32I GATATC 3 cut(s) 76, 598, 1120
Eco47I GGWCC 1 cut(s) 799
Eco57I CTGAAG 2 cut(s) 678, 1004
EcoO109I RGGNCCY 1 cut(s) 545
EcoRI GAATTC 2 cut(s) 888, 907
EcoRII CCWGG 3 cut(s) 84, 137, 801
EcoRV GATATC 3 cut(s) 76, 598, 1120
EcoT14I CCWWGG 1 cut(s) 642
EcoT38I GRGCYC 1 cut(s) 548
ErhI CCWWGG 1 cut(s) 642
FaeI CATG 7 cut(s) 41, 119, 125, 334, 467, 728, 1018
FatI CATG 7 cut(s) 37, 115, 121, 330, 463, 724, 1014
FauNDI CATATG 1 cut(s) 903
FbaI TGATCA 2 cut(s) 118, 970
Fnu4HI GCNGC 1 cut(s) 1077
FokI GGATG 3 cut(s) 188, 704, 960
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 1 cut(s) 1077
GluI GCNGC 1 cut(s) 1077
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 6 cut(s) 503, 509, 546, 773, 899, 1013
HapII CCGG 1 cut(s) 431
Hin1II CATG 7 cut(s) 41, 119, 125, 334, 467, 728, 1018
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 4 cut(s) 10, 32, 233, 1040
HpaII CCGG 1 cut(s) 431
HphI GGTGA 2 cut(s) 74, 290
Hpy166II GTNNAC 3 cut(s) 680, 869, 1085
Hpy188III TCNNGA 3 cut(s) 617, 725, 764
Hpy8I GTNNAC 3 cut(s) 680, 869, 1085
HpyAV CCTTC 8 cut(s) 140, 459, 653, 682, 761, 828, 979, 1110
HpyCH4III ACNGT 2 cut(s) 166, 187
HpyF10VI GCNNNNNNNGC 1 cut(s) 1073
HpyF3I CTNAG 4 cut(s) 45, 255, 732, 912
Hsp92II CATG 7 cut(s) 41, 119, 125, 334, 467, 728, 1018
Ksp22I TGATCA 2 cut(s) 118, 970
LmnI GCTCC 2 cut(s) 668, 695
LweI GCATC 3 cut(s) 166, 259, 485
MboII GAAGA 5 cut(s) 614, 851, 854, 857, 941
MflI RGATCY 4 cut(s) 88, 339, 520, 805
MhlI GDGCHC 2 cut(s) 46, 548
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 6 cut(s) 105, 284, 345, 495, 710, 1094
MslI CAYNNNNRTG 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 1079
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 3 cut(s) 86, 139, 803
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 86, 139, 803
MwoI GCNNNNNNNGC 1 cut(s) 1073
NdeI CATATG 1 cut(s) 903
NlaIII CATG 7 cut(s) 41, 119, 125, 334, 467, 728, 1018
NlaIV GGNNCC 5 cut(s) 90, 522, 546, 664, 772
NmeAIII GCCGAG 1 cut(s) 529
PagI TCATGA 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PctI GAATGC 1 cut(s) 256
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 4 cut(s) 10, 32, 233, 1040
PflMI CCANNNNNTGG 2 cut(s) 757, 884
PkrI GCNGC 1 cut(s) 1078
Ple19I CGATCG 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 84, 137, 801
PspGI CCWGG 3 cut(s) 84, 137, 801
PspN4I GGNNCC 5 cut(s) 90, 522, 546, 664, 772
PspOMI GGGCCC 1 cut(s) 544
PspPI GGNCC 5 cut(s) 544, 545, 771, 799, 898
PstI CTGCAG 2 cut(s) 213, 687
PsuI RGATCY 4 cut(s) 88, 339, 520, 805
PvuI CGATCG 1 cut(s) 17
RsaI GTAC 3 cut(s) 218, 868, 1126
RsaNI GTAC 3 cut(s) 217, 867, 1125
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 6 cut(s) 105, 284, 345, 495, 710, 1094
SatI GCNGC 1 cut(s) 1077
Sau96I GGNCC 5 cut(s) 544, 545, 771, 799, 898
ScrFI CCNGG 3 cut(s) 86, 139, 803
SduI GDGCHC 2 cut(s) 46, 548
SfaNI GCATC 3 cut(s) 166, 259, 485
SfcI CTRYAG 2 cut(s) 209, 683
SinI GGWCC 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
SsiI CCGC 1 cut(s) 1077
StyD4I CCNGG 3 cut(s) 84, 137, 801
StyI CCWWGG 1 cut(s) 642
TaaI ACNGT 2 cut(s) 166, 187
TaqI TCGA 2 cut(s) 13, 600
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 1079
TfiI GAWTC 4 cut(s) 10, 32, 233, 1040
Tru1I TTAA 6 cut(s) 105, 284, 345, 495, 710, 1094
Tru9I TTAA 6 cut(s) 105, 284, 345, 495, 710, 1094
TscAI CASTG 4 cut(s) 517, 579, 743, 1110
TspDTI ATGAA 2 cut(s) 263, 920
TspRI CASTG 4 cut(s) 517, 579, 743, 1110
Van91I CCANNNNNTGG 2 cut(s) 757, 884
VpaK11BI GGWCC 1 cut(s) 799
XapI RAATTY 2 cut(s) 888, 907
XmnI GAANNNNTTC 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.