Rorug05G0082100

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004982
Physical Location & Seq
Forward (+)
7096939 .. 7100256
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0082100.1

Sequence Viewer

Length: 1458 bp
ATGCTGGACTTAAATCTGTTTAGAGAAAGGAAGGGCCACGACCCCGATGTCATCCGCAAGTCGCATGGCCGTCGTTTCAAGAGCGTTGATCTTGTTGACAAGGTGATCGACCTCGACCAAGAATGGTGCCAAAGACAATTCGAGCTCGTGAATCTCAGGAAAGAGTTCAACCAGACCAACAAGGAAATTGGTCAGCTCAGAATTAAAGAAGGTGAGTTGATTAAAAAGGCAGAGGAAAAGAAGAGGTTGATCGCAGAGAAAGAAGCTGAAGTTCAAGAAGCTTTAGCACAACTGAATTCAAGCTTGGAACAAATTGGGAACCTTGTCCATGATTCAGTTCCCGTCAGCGAAGATGAGGCAAACAATGAAGTGGTTAGATCATGGGGAGAGAACCGGGTGGAGCCAGCACTGAAAAATCATGTCGAGCTTTGTAAGCTTCTTGGGATTGCTGACTTTGAGAAAGGTGCTAAAGTGGCTGGATCCAGAGGTTATTACATTGAAGGCGTTGGTGTAGATCTTCATGAAGCTCTCAAAAACTTTGCTCTAGATTTTCTTGAGGACAAGGGATTCAAAAAAATGATTCCTCCATTTTCTATGAGACAAGATGTCATGGCCAAATGTGCTCAACTACAGGAATTCGATAATGAACTCTATAAGGTAACTGGTGAAGGAGACGATAAGTATTTGATTGCTACCGCAGAACAGCCTCTATGTGCTCGTCACAAGGATGGATGGTTCCATCCCTTCCAGCTACCCATAAAATATGCTGGATTTTCATATTGCTTCCGGACAGAAGCTGGCTCGCATGGAAGAGATACATTGGGGCTTTTTAGGACTCATGAGTTTGCCAAAGTAGAACAATTCTGTATTACCAGCCCAAATGAAAATGACTCTTGCGATATGCATGAGGAGATGATCAGAAACTCAGAGGAGTTCTATAAAGCTCTCAAGATTCCATATCGTGTAGTCAATATTGTTTCTGGTGCTTTGAATGGTGCAGCAGCAAAGAAGTATGACTTAGAAGGTTGGTTTCCTGCATCTCAGACATATAGGGAACTAGTCTCATGTTCAAACTGTACAGACTATCAGTCCAGAAAATTAGAAATTCGATATGGACAGACAGGCAATGAGAAGACAAAGCAGTACGTTCACATGTTGAACTCCACTCTTACTGCAATTGGGAGAACCATGTGCTGCATTCTCGAAAGCTACCAAATGAAAGATGGTGTCGAGCTGAGGAAAAACGCCTTGAACCGTGCAAAATTAACAGAAGATCAAGTATTGCAGAAAATCGAGGAAAAAACTGCAGCAAGGAAGAACAAAAAGTACGAAGATTCAGATAAAATCAGAAAAGATTTAGCTGCTGTGGGAATCACTCTTATGGACAACCCAGATGGGACAACATGGAGACCAGCTGTACCTCTTGCAATTCAAGAGCAGCCGGTGGCATCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

485

Amino Acids

55.39

Weight (kDa)

6.09

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Seryl_tRNA_N PF02403 1 - 109 2.4e-20 Seryl-tRNA synthetase N-terminal domain
tRNA-synt_2b PF00587 218 - 404 4.9e-39 tRNA synthetase class II core domain (G, H, P, S and T)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 126
AccIII TCCGGA 1 cut(s) 786
AciI CCGC 2 cut(s) 55, 696
AclWI GGATC 2 cut(s) 474, 487
AcoI YGGCCR 2 cut(s) 67, 612
AcsI RAATTY 3 cut(s) 295, 635, 1104
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 4 cut(s) 1078, 1145, 1328, 1419
AflIII ACRYGT 1 cut(s) 1152
AhdI GACNNNNNGTC 2 cut(s) 605, 1087
AhlI ACTAGT 1 cut(s) 1057
AjuI GAANNNNNNNTTGG 2 cut(s) 287, 319
AloI GAACNNNNNNTCC 2 cut(s) 719, 751
Alw21I GWGCWC 3 cut(s) 147, 625, 718
Alw26I GTCTC 4 cut(s) 592, 666, 1066, 1402
AlwI GGATC 2 cut(s) 474, 487
AlwNI CAGNNNCTG 1 cut(s) 797
Aor13HI TCCGGA 1 cut(s) 786
AoxI GGCC 3 cut(s) 34, 67, 612
ApeKI GCWGC 6 cut(s) 998, 1001, 1194, 1307, 1361, 1438
ApoI RAATTY 3 cut(s) 295, 635, 1104
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 395
AsuHPI GGTGA 3 cut(s) 115, 224, 677
BalI TGGCCA 1 cut(s) 614
BamHI GGATCC 1 cut(s) 479
BanI GGYRCC 1 cut(s) 126
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 1139
Bbv12I GWGCWC 3 cut(s) 147, 625, 718
BbvCI CCTCAGC 1 cut(s) 1235
BbvI GCAGC 6 cut(s) 1010, 1013, 1181, 1319, 1348, 1450
BccI CCATC 5 cut(s) 722, 726, 747, 1217, 1388
BceAI ACGGC 1 cut(s) 54
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BclI TGATCA 1 cut(s) 915
BcnI CCSGG 1 cut(s) 395
BcoDI GTCTC 4 cut(s) 592, 666, 1066, 1402
BcuI ACTAGT 1 cut(s) 1057
BfaI CTAG 2 cut(s) 545, 1058
BfmI CTRYAG 2 cut(s) 629, 1305
BglII AGATCT 1 cut(s) 514
BisI GCNGC 6 cut(s) 999, 1002, 1195, 1308, 1362, 1439
BlsI GCNGC 6 cut(s) 1000, 1003, 1196, 1309, 1363, 1440
Bme1390I CCNGG 1 cut(s) 395
BmeRI GACNNNNNGTC 2 cut(s) 605, 1087
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 5 cut(s) 128, 320, 402, 481, 737
BmrFI CCNGG 1 cut(s) 395
BmsI GCATC 1 cut(s) 1046
BpiI GAAGAC 1 cut(s) 1139
Bpu10I CCTNAGC 1 cut(s) 1235
BpuEI CTTGAG 2 cut(s) 575, 932
BpuMI CCSGG 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 1402
BsaWI WCCGGW 1 cut(s) 786
Bse118I RCCGGY 1 cut(s) 1441
Bse1I ACTGG 1 cut(s) 667
Bse3DI GCAATG 1 cut(s) 1132
BseAI TCCGGA 1 cut(s) 786
BseGI GGATG 4 cut(s) 51, 733, 737, 739
BseMI GCAATG 1 cut(s) 1132
BseMII CTCAG 5 cut(s) 169, 211, 939, 1055, 1226
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 2 cut(s) 923, 944
BseXI GCAGC 6 cut(s) 1010, 1013, 1181, 1319, 1348, 1450
BsgI GTGCAG 1 cut(s) 1017
BshFI GGCC 3 cut(s) 36, 69, 614
BshNI GGYRCC 1 cut(s) 126
BsiHKAI GWGCWC 3 cut(s) 147, 625, 718
BsiSI CCGG 3 cut(s) 394, 787, 1442
BslFI GGGAC 1 cut(s) 1411
BsmAI GTCTC 4 cut(s) 592, 666, 1066, 1402
BsmBI CGTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 1411
BsmI GAATGC 1 cut(s) 1197
BsnI GGCC 3 cut(s) 36, 69, 614
Bso31I GGTCTC 1 cut(s) 1402
Bsp1286I GDGCHC 3 cut(s) 147, 625, 718
Bsp13I TCCGGA 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 1076
Bsp143I GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
BspACI CCGC 2 cut(s) 55, 696
BspANI GGCC 3 cut(s) 36, 69, 614
BspCNI CTCAG 5 cut(s) 168, 210, 938, 1054, 1227
BspEI TCCGGA 1 cut(s) 786
BspHI TCATGA 2 cut(s) 520, 838
BspLI GGNNCC 5 cut(s) 128, 320, 402, 481, 737
BspMAI CTGCAG 1 cut(s) 1309
BspPI GGATC 2 cut(s) 474, 487
BspT107I GGYRCC 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 1402
BsrDI GCAATG 1 cut(s) 1132
BsrFI RCCGGY 1 cut(s) 1441
BsrGI TGTACA 1 cut(s) 1076
BsrI ACTGG 1 cut(s) 667
BssAI RCCGGY 1 cut(s) 1441
BssMI GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 1076, 1256
Bst6I CTCTTC 2 cut(s) 236, 805
BstAUI TGTACA 1 cut(s) 1076
BstC8I GCNNGC 3 cut(s) 405, 799, 803
BstDEI CTNAG 6 cut(s) 155, 197, 925, 1018, 1041, 1235
BstF5I GGATG 4 cut(s) 51, 733, 737, 739
BstKTI GATC 8 cut(s) 91, 108, 252, 380, 482, 517, 918, 1276
BstMAI GTCTC 4 cut(s) 592, 666, 1066, 1402
BstMBI GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
BstMWI GCNNNNNNNGC 3 cut(s) 433, 473, 620
BstNSI RCATGY 1 cut(s) 1156
BstSCI CCNGG 1 cut(s) 393
BstSFI CTRYAG 2 cut(s) 629, 1305
BstV1I GCAGC 6 cut(s) 1010, 1013, 1181, 1319, 1348, 1450
BstV2I GAAGAC 1 cut(s) 1139
BstX2I RGATCY 2 cut(s) 479, 514
BstYI RGATCY 2 cut(s) 479, 514
BsuRI GGCC 3 cut(s) 36, 69, 614
BtsCI GGATG 4 cut(s) 51, 733, 737, 739
BtsIMutI CAGTG 1 cut(s) 407
Cac8I GCNNGC 3 cut(s) 405, 799, 803
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 2 cut(s) 520, 838
Cfr10I RCCGGY 1 cut(s) 1441
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 4 cut(s) 1077, 1144, 1327, 1418
CviQI GTAC 4 cut(s) 1077, 1144, 1327, 1418
DdeI CTNAG 6 cut(s) 155, 197, 925, 1018, 1041, 1235
DpnI GATC 8 cut(s) 90, 107, 251, 379, 481, 516, 917, 1275
DpnII GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 2 cut(s) 605, 1087
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 67, 612
Eam1104I CTCTTC 2 cut(s) 236, 805
Eam1105I GACNNNNNGTC 2 cut(s) 605, 1087
EarI CTCTTC 2 cut(s) 236, 805
Ecl136II GAGCTC 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 147
Eco31I GGTCTC 1 cut(s) 1402
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 288
EcoICRI GAGCTC 1 cut(s) 145
EcoRI GAATTC 2 cut(s) 295, 635
EcoT22I ATGCAT 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 147
Esp3I CGTCTC 1 cut(s) 666
FalI AAGNNNNNCTT 2 cut(s) 1001, 1033
FaqI GGGAC 1 cut(s) 1411
FbaI TGATCA 1 cut(s) 915
Fnu4HI GCNGC 6 cut(s) 999, 1002, 1195, 1308, 1362, 1439
FokI GGATG 4 cut(s) 38, 726, 740, 744
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 6 cut(s) 999, 1002, 1195, 1308, 1362, 1439
FspBI CTAG 2 cut(s) 545, 1058
GluI GCNGC 6 cut(s) 999, 1002, 1195, 1308, 1362, 1439
HaeIII GGCC 3 cut(s) 36, 69, 614
HapII CCGG 3 cut(s) 394, 787, 1442
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HindIII AAGCTT 3 cut(s) 279, 301, 434
HinfI GANTC 9 cut(s) 151, 332, 567, 580, 835, 890, 952, 1334, 1371
HpaII CCGG 3 cut(s) 394, 787, 1442
HphI GGTGA 3 cut(s) 115, 224, 677
Hpy166II GTNNAC 2 cut(s) 97, 1150
Hpy188I TCNGA 6 cut(s) 200, 920, 928, 1044, 1339, 1349
Hpy8I GTNNAC 2 cut(s) 97, 1150
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 6 cut(s) 25, 203, 494, 662, 754, 1016
HpyCH4III ACNGT 2 cut(s) 1076, 1256
HpyCH4IV ACGT 1 cut(s) 1146
HpyCH4V TGCA 9 cut(s) 904, 998, 1037, 1175, 1197, 1259, 1285, 1307, 1427
HpyF10VI GCNNNNNNNGC 3 cut(s) 433, 473, 620
HpyF3I CTNAG 6 cut(s) 155, 197, 925, 1018, 1041, 1235
HpySE526I ACGT 1 cut(s) 1146
Kpn2I TCCGGA 1 cut(s) 786
Ksp22I TGATCA 1 cut(s) 915
Kzo9I GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 6 cut(s) 1010, 1013, 1181, 1319, 1348, 1450
LweI GCATC 1 cut(s) 1046
MaeI CTAG 2 cut(s) 545, 1058
MaeII ACGT 1 cut(s) 1146
MaeIII GTNAC 2 cut(s) 658, 719
MalI GATC 8 cut(s) 90, 107, 251, 379, 481, 516, 917, 1275
MboI GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
MboII GAAGA 8 cut(s) 253, 362, 509, 822, 1144, 1283, 1327, 1343
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 2 cut(s) 479, 514
MhlI GDGCHC 3 cut(s) 147, 625, 718
MlsI TGGCCA 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 614
MlyI GAGTC 2 cut(s) 829, 884
Mox20I TGGCCA 1 cut(s) 614
Mph1103I ATGCAT 1 cut(s) 906
MroI TCCGGA 1 cut(s) 786
MroXI GAANNNNTTC 1 cut(s) 164
MscI TGGCCA 1 cut(s) 614
MseI TTAA 4 cut(s) 11, 204, 222, 1265
MslI CAYNNNNRTG 2 cut(s) 726, 1379
Msp20I TGGCCA 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 1415
MspI CCGG 3 cut(s) 394, 787, 1442
MspR9I CCNGG 1 cut(s) 395
MunI CAATTG 1 cut(s) 1176
Mva1269I GAATGC 1 cut(s) 1197
MwoI GCNNNNNNNGC 3 cut(s) 433, 473, 620
NciI CCSGG 1 cut(s) 395
NdeII GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
NlaIV GGNNCC 5 cut(s) 128, 320, 402, 481, 737
NmuCI GTSAC 1 cut(s) 719
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 1 cut(s) 1156
PagI TCATGA 2 cut(s) 520, 838
PciI ACATGT 1 cut(s) 1152
PctI GAATGC 1 cut(s) 1197
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 7 cut(s) 151, 332, 567, 580, 952, 1334, 1371
PkrI GCNGC 6 cut(s) 1000, 1003, 1196, 1309, 1363, 1440
PleI GAGTC 2 cut(s) 829, 884
PpsI GAGTC 2 cut(s) 829, 884
PscI ACATGT 1 cut(s) 1152
Psp124BI GAGCTC 1 cut(s) 147
PspN4I GGNNCC 5 cut(s) 128, 320, 402, 481, 737
PspPI GGNCC 1 cut(s) 34
PsrI GAACNNNNNNTAC 2 cut(s) 1310, 1342
PstI CTGCAG 1 cut(s) 1309
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 479, 514
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 4 cut(s) 1078, 1145, 1328, 1419
RsaNI GTAC 4 cut(s) 1077, 1144, 1327, 1418
RseI CAYNNNNRTG 2 cut(s) 726, 1379
SacI GAGCTC 1 cut(s) 147
SaqAI TTAA 4 cut(s) 11, 204, 222, 1265
SatI GCNGC 6 cut(s) 999, 1002, 1195, 1308, 1362, 1439
Sau3AI GATC 8 cut(s) 88, 105, 249, 377, 479, 514, 915, 1273
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 2 cut(s) 829, 884
ScrFI CCNGG 1 cut(s) 395
SduI GDGCHC 3 cut(s) 147, 625, 718
SfaNI GCATC 1 cut(s) 1046
SfcI CTRYAG 2 cut(s) 629, 1305
SmiMI CAYNNNNRTG 2 cut(s) 726, 1379
SmlI CTYRAG 2 cut(s) 554, 947
SmoI CTYRAG 2 cut(s) 554, 947
SpeI ACTAGT 1 cut(s) 1057
SsiI CCGC 2 cut(s) 55, 696
SspI AATATT 1 cut(s) 973
SspMI CTAG 2 cut(s) 545, 1058
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 393
TaaI ACNGT 2 cut(s) 1076, 1256
TaiI ACGT 1 cut(s) 1149
TaqI TCGA 9 cut(s) 108, 114, 141, 423, 639, 1108, 1203, 1230, 1293
TatI WGTACW 1 cut(s) 1076
TfiI GAWTC 7 cut(s) 151, 332, 567, 580, 952, 1334, 1371
Tru1I TTAA 4 cut(s) 11, 204, 222, 1265
Tru9I TTAA 4 cut(s) 11, 204, 222, 1265
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 6 cut(s) 998, 1001, 1194, 1307, 1361, 1438
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 7 cut(s) 381, 509, 537, 660, 765, 897, 1232
TspRI CASTG 1 cut(s) 414
XapI RAATTY 3 cut(s) 295, 635, 1104
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 1 cut(s) 1156
XcmI CCANNNNNNNNNTGG 1 cut(s) 1220
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 2 cut(s) 545, 1058
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.