Rroxscaffold_1G00055700

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004982
Physical Location & Seq
Forward (+)
77355145 .. 77362682
7538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00055700.1

Sequence Viewer

Length: 2265 bp
ATGAACTACCCTCCTGTTGAAGAAGCATCAAAAGATGAAAGAAAGTACCAGGAACCTGAAAATGTTGCTTCATCAATTGATTCCGGGAAGTCTATGCTGGTTCACAATCCTAGTGAAGTTGATCATCAGTCCACATCTAACAATGCATTTGCTCCTACTTATGCTGACCCAATGTCCAATGGAATTGATTTGGGGTACCTTGAGCCACCTGTGCAGCCTCAGAGAATCTACTACTCGGAAAGAATTCCCAGGGAGCAGGCAGAGTTGCTGAATCGATCGTCAAAGTCTGATGATTCGCATGGTGCTCAGTTTCTCGTATCTCATTCACGCTCTGATATCATTCACCAGGATCCAATTACAGGAGTTAAGAAATTGCATGATCATGCAAACCTTCCTCCCAGGTCTGAACAACAATCCTCCTCAACTGTAAATGTGGATGCTCAAACTGTTGATGATGGACTTGCTCAACTGCAGAAGTACAAAGAGTTTGCTGATTCAATTTGTGAGATGAATGCTAAGCTTTTGCAAGATGCAGATGGTGAATTAAAGCCAGCATTGCCAAACTCTGTGGATACTAAAGAGATCGCGAACAGGGATAAAATCCTTAAATCTGACCAAGAAACAAACTGCCTCAAAGGTGGCCATAAGAAACTTGTGGTTGATGACATTGCAGAAGCTATATCAGAGTATCCGGCTGTAAGTCAGATACCTTCTGTGAAGCACCATGAGGTTTCTGCATCTAATCATTCAGAGCTTAATCAGGCCGAGGCCACTGGCAAGGATCCAAATACTGCTGACAATATGGGCCGTGCACAAGTTGGTATATCTACTCCAGTGCAGGGAGACATAATTATTGATATCGAAGAACGATTTCCTCGTGATTTCCTGTCTGATATATTCTCCAAGGCAATACTTTCTGAAGGCTCCCCTGATGTTGGTTTACTGCAGAAGGATGGAGCTGGTTTGAGCTTAAATATGGAAAATCATGATCCTAAGCGCTGGTCATATTTCCAAAAGTTGGCTCAAGAAGGGGCTGATCAACAAGATGTTTCTCTTATGGACCAGGATCTTGGTTTTCCTTCTGCAATTAGAAATGTTGAAGAAGAAGATGGTAAATCTTATCATCGTACACCTTTACGAACAGATGGAGTTCCAATGGCCCATATGAATTCTCAGCCTAATTTTGGAGAAGATATTAGCAAAGAAACTGGACTGACTAAAGCTAATTATGATCATCCGCAACTGAAGGAAACAGAAAGTATGCAGTTTGAGGCCATGATGGAAAACTTACGAGTGCCAGAATCAGATTATGAGGAAGGAAAGTTTGCAAGTAGAACCGCTGGTTTACCTCCTTTAAATCCTTCACTGGGAGATTTTGATATCAGTACCTTGCAGTTGATAAAGAATGAAGATCTAGAACAGATGAAAGAACTGGGTTCTGGCACCTTTGGGACTGTGTATCATGGAAAATGGAGGGGGAGTGATGTTGCCATTAAAAGACTAAATAAGAGCTGCTTCACTGGGAGATCATCAGAGCAAGAGAGACTGTCTATAGAGTTCTGGCGGGAAGCTGACATTCTTTCAAAGCTACACCATCCAAATGTGGTGGCATTTTATGGTGTAGTGCAAGATGGGCCAGGGGGAACACTAGCTACCGTAACTGAGTACATGGTTGATGGTTCTCTTAGGCATGTCTTACTTCGAAAAGATAGGTATCTTGATCGTCGCAAGCGATTAATAATTGCCATGGATGCAGCATTTGGAATGGAATATTTGCACTCAAAGAATATTGTTCATTTTGACCTGAAGTGTGACAACTTACTTGTGAACCTCAAAGATCCTCAACGGCCAATTTGCAAGGTCGGTGATTTTGGCCTTTCAAAGATTAAGCGAAATACCCTGGTTTCTGGTGGTGTACGTGGAACATTACCATGGATGGCTCCAGAGTTGCTGAATGGAAGCAGCACTAAGGTCTCCGAAAAGGTGGATATTTTCTCATTTGGTATTGTCTTATGGGAGATTCTTACTGGAGAGGAGCCGTATGCCAACATGCACTATGGAGCAATTATAGGCGGTATTGTGAACAACACATTGAGACCCACCATTCCAAGTTACTGTGATCCAGAATGGAGAACGCTTATGGAGCAGTGTTGGGCTCCTAATCCTGCTGCCAGGCCATCGTTCACAGAAATTGCTAGTTGCTTACGAGTTATGACAACAGCAGCTAGCCAACCCAAAGCACAGGGTCACAAGGCATCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

754

Amino Acids

83.76

Weight (kDa)

5.29

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 473 - 735 5.4e-63 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 473 - 732 8.4e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 195
AccB1I GGYRCC 2 cut(s) 195, 1442
AccB7I CCANNNNNTGG 1 cut(s) 1018
AccII CGCG 1 cut(s) 587
AciI CCGC 4 cut(s) 1238, 1338, 1564, 2073
AclWI GGATC 8 cut(s) 344, 357, 776, 789, 983, 1074, 1832, 2114
AcoI YGGCCR 2 cut(s) 640, 1847
AcsI RAATTY 2 cut(s) 243, 1168
AcuI CTGAAG 3 cut(s) 939, 1265, 1826
AfaI GTAC 7 cut(s) 47, 197, 479, 1129, 1387, 1667, 1917
AfeI AGCGCT 1 cut(s) 998
AfiI CCNNNNNNNGG 6 cut(s) 359, 839, 935, 1018, 1184, 1367
AgsI TTSAA 5 cut(s) 20, 498, 1100, 1584, 1881
AhdI GACNNNNNGTC 1 cut(s) 172
AjnI CCWGG 8 cut(s) 48, 248, 345, 398, 1062, 1636, 1899, 2171
Alw21I GWGCWC 2 cut(s) 307, 814
Alw26I GTCTC 4 cut(s) 837, 1537, 1978, 2089
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 8 cut(s) 344, 357, 776, 789, 983, 1074, 1832, 2114
Aor51HI AGCGCT 1 cut(s) 998
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 6 cut(s) 214, 1512, 1754, 1962, 2168, 2222
ApoI RAATTY 2 cut(s) 243, 1168
AseI ATTAAT 1 cut(s) 1736
Asp700I GAANNNNTTC 2 cut(s) 243, 870
Asp718I GGTACC 1 cut(s) 195
AspLEI GCGC 1 cut(s) 999
AspS9I GGNCC 4 cut(s) 805, 1060, 1159, 1634
AsuC2I CCSGG 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 335, 551, 1877
AsuII TTCGAA 1 cut(s) 1702
AsuNHI GCTAGC 1 cut(s) 2225
AvaII GGWCC 1 cut(s) 1060
BaeGI GKGCMC 1 cut(s) 814
BalI TGGCCA 1 cut(s) 642
BamHI GGATCC 2 cut(s) 349, 781
BanI GGYRCC 2 cut(s) 195, 1442
BanII GRGCYC 1 cut(s) 2158
BauI CACGAG 1 cut(s) 876
Bbv12I GWGCWC 2 cut(s) 307, 814
BbvI GCAGC 6 cut(s) 226, 1499, 1766, 1974, 2155, 2234
BceAI ACGGC 3 cut(s) 792, 1862, 2023
BciT130I CCWGG 8 cut(s) 50, 250, 347, 400, 1064, 1638, 1901, 2173
BciVI GTATCC 2 cut(s) 565, 699
BclI TGATCA 4 cut(s) 121, 379, 1036, 1231
BcnI CCSGG 1 cut(s) 85
BcoDI GTCTC 4 cut(s) 837, 1537, 1978, 2089
BfaI CTAG 5 cut(s) 111, 1415, 1649, 2196, 2226
BfmI CTRYAG 3 cut(s) 470, 944, 1551
BfoI RGCGCY 1 cut(s) 1000
BfuI GTATCC 2 cut(s) 565, 699
BglII AGATCT 1 cut(s) 1411
BisI GCNGC 6 cut(s) 215, 1513, 1755, 1963, 2169, 2223
BlpI GCTNAGC 1 cut(s) 516
BlsI GCNGC 6 cut(s) 216, 1514, 1756, 1964, 2170, 2224
Bme1390I CCNGG 9 cut(s) 50, 85, 250, 347, 400, 1064, 1638, 1901, 2173
Bme18I GGWCC 1 cut(s) 1060
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgT120I GGNCC 4 cut(s) 805, 1060, 1159, 1634
BmiI GGNNCC 9 cut(s) 54, 197, 351, 783, 925, 1444, 1941, 2037, 2157
BmrFI CCNGG 9 cut(s) 50, 85, 250, 347, 400, 1064, 1638, 1901, 2173
BmrI ACTGGG 3 cut(s) 1376, 1442, 1530
BmsI GCATC 5 cut(s) 35, 427, 520, 746, 1741
BmtI GCTAGC 1 cut(s) 2229
BmuI ACTGGG 3 cut(s) 1376, 1442, 1530
BpmI CTGGAG 3 cut(s) 816, 1926, 2049
Bpu10I CCTNAGC 1 cut(s) 993
Bpu1102I GCTNAGC 1 cut(s) 516
Bpu14I TTCGAA 1 cut(s) 1702
BpuEI CTTGAG 2 cut(s) 221, 1008
BpuMI CCSGG 1 cut(s) 85
Bsa29I ATCGAT 1 cut(s) 274
BsaAI YACGTR 1 cut(s) 1919
BsaBI GATNNNNATC 1 cut(s) 1713
BsaI GGTCTC 2 cut(s) 1978, 2089
BsaJI CCNNGG 9 cut(s) 248, 249, 398, 765, 903, 1637, 1746, 1899, 1931
BsaXI ACNNNNNCTCC 2 cut(s) 1465, 1495
Bsc4I CCNNNNNNNGG 6 cut(s) 359, 839, 935, 1018, 1184, 1367
Bse1I ACTGG 7 cut(s) 778, 833, 1213, 1371, 1437, 1525, 2032
Bse3DI GCAATG 2 cut(s) 554, 666
Bse8I GATNNNNATC 1 cut(s) 1713
BseBI CCWGG 8 cut(s) 50, 250, 347, 400, 1064, 1638, 1901, 2173
BseCI ATCGAT 1 cut(s) 274
BseDI CCNNGG 9 cut(s) 248, 249, 398, 765, 903, 1637, 1746, 1899, 1931
BseGI GGATG 6 cut(s) 442, 958, 1234, 1594, 1756, 1941
BseJI GATNNNNATC 1 cut(s) 1713
BseLI CCNNNNNNNGG 6 cut(s) 359, 839, 935, 1018, 1184, 1367
BseMI GCAATG 2 cut(s) 554, 666
BseMII CTCAG 4 cut(s) 233, 320, 1187, 1653
BseNI ACTGG 7 cut(s) 778, 833, 1213, 1371, 1437, 1525, 2032
BseRI GAGGAG 2 cut(s) 409, 2048
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 6 cut(s) 226, 1499, 1766, 1974, 2155, 2234
BsgI GTGCAG 2 cut(s) 233, 857
Bsh1236I CGCG 1 cut(s) 587
Bsh1285I CGRYCG 1 cut(s) 278
BshNI GGYRCC 2 cut(s) 195, 1442
BshVI ATCGAT 1 cut(s) 274
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 307, 814
BsiSI CCGG 2 cut(s) 84, 692
BslFI GGGAC 1 cut(s) 1465
BslI CCNNNNNNNGG 6 cut(s) 359, 839, 935, 1018, 1184, 1367
BsmAI GTCTC 4 cut(s) 837, 1537, 1978, 2089
BsmFI GGGAC 1 cut(s) 1465
BsmI GAATGC 1 cut(s) 517
Bso31I GGTCTC 2 cut(s) 1978, 2089
Bsp119I TTCGAA 1 cut(s) 1702
Bsp1286I GDGCHC 3 cut(s) 307, 814, 2158
Bsp1720I GCTNAGC 1 cut(s) 516
Bsp19I CCATGG 2 cut(s) 1746, 1931
Bsp68I TCGCGA 1 cut(s) 587
BspACI CCGC 4 cut(s) 1238, 1338, 1564, 2073
BspCNI CTCAG 4 cut(s) 232, 319, 1186, 1654
BspDI ATCGAT 1 cut(s) 274
BspFNI CGCG 1 cut(s) 587
BspHI TCATGA 1 cut(s) 985
BspLI GGNNCC 9 cut(s) 54, 197, 351, 783, 925, 1444, 1941, 2037, 2157
BspMAI CTGCAG 2 cut(s) 474, 948
BspOI GCTAGC 1 cut(s) 2229
BspPI GGATC 8 cut(s) 344, 357, 776, 789, 983, 1074, 1832, 2114
BspT104I TTCGAA 1 cut(s) 1702
BspT107I GGYRCC 2 cut(s) 195, 1442
BspTNI GGTCTC 2 cut(s) 1978, 2089
BsrDI GCAATG 2 cut(s) 554, 666
BsrI ACTGG 7 cut(s) 778, 833, 1213, 1371, 1437, 1525, 2032
BssECI CCNNGG 9 cut(s) 248, 249, 398, 765, 903, 1637, 1746, 1899, 1931
BssSI CACGAG 1 cut(s) 876
BssT1I CCWWGG 3 cut(s) 903, 1746, 1931
Bst2BI CACGAG 1 cut(s) 876
Bst2UI CCWGG 8 cut(s) 50, 250, 347, 400, 1064, 1638, 1901, 2173
Bst4CI ACNGT 6 cut(s) 427, 448, 1456, 1548, 1657, 2117
BstBAI YACGTR 1 cut(s) 1919
BstBI TTCGAA 1 cut(s) 1702
BstC8I GCNNGC 4 cut(s) 258, 552, 1730, 2227
BstDEI CTNAG 8 cut(s) 219, 306, 516, 993, 1173, 1662, 1685, 1968
BstDSI CCRYGG 2 cut(s) 1746, 1931
BstF5I GGATG 6 cut(s) 442, 958, 1234, 1594, 1756, 1941
BstFNI CGCG 1 cut(s) 587
BstH2I RGCGCY 1 cut(s) 1000
BstHHI GCGC 1 cut(s) 999
BstMAI GTCTC 4 cut(s) 837, 1537, 1978, 2089
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 5 cut(s) 211, 556, 1633, 1751, 2143
BstNI CCWGG 8 cut(s) 50, 250, 347, 400, 1064, 1638, 1901, 2173
BstNSI RCATGY 2 cut(s) 1694, 2053
BstSCI CCNGG 9 cut(s) 48, 83, 248, 345, 398, 1062, 1636, 1899, 2171
BstSFI CTRYAG 3 cut(s) 470, 944, 1551
BstSLI GKGCMC 1 cut(s) 814
BstUI CGCG 1 cut(s) 587
BstV1I GCAGC 6 cut(s) 226, 1499, 1766, 1974, 2155, 2234
BstX2I RGATCY 5 cut(s) 349, 781, 1066, 1411, 1837
BstXI CCANNNNNNTGG 1 cut(s) 1070
BstYI RGATCY 5 cut(s) 349, 781, 1066, 1411, 1837
Bsu15I ATCGAT 1 cut(s) 274
BsuI GTATCC 2 cut(s) 565, 699
BsuTUI ATCGAT 1 cut(s) 274
BtgI CCRYGG 2 cut(s) 1746, 1931
BtsCI GGATG 6 cut(s) 442, 958, 1234, 1594, 1756, 1941
BtsI GCAGTG 1 cut(s) 2153
BtsIMutI CAGTG 5 cut(s) 771, 840, 1364, 1518, 2153
BtuMI TCGCGA 1 cut(s) 587
Cac8I GCNNGC 4 cut(s) 258, 552, 1730, 2227
CciI TCATGA 1 cut(s) 985
CfoI GCGC 1 cut(s) 999
Cfr13I GGNCC 4 cut(s) 805, 1060, 1159, 1634
ClaI ATCGAT 1 cut(s) 274
Csp6I GTAC 7 cut(s) 46, 196, 478, 1128, 1386, 1666, 1916
CspCI CAANNNNNGTGG 4 cut(s) 549, 584, 1587, 1622
CviQI GTAC 7 cut(s) 46, 196, 478, 1128, 1386, 1666, 1916
DdeI CTNAG 8 cut(s) 219, 306, 516, 993, 1173, 1662, 1685, 1968
DraI TTTAAA 1 cut(s) 1356
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 2 cut(s) 640, 1847
Eam1105I GACNNNNNGTC 1 cut(s) 172
Eco130I CCWWGG 3 cut(s) 903, 1746, 1931
Eco24I GRGCYC 1 cut(s) 2158
Eco31I GGTCTC 2 cut(s) 1978, 2089
Eco32I GATATC 3 cut(s) 337, 859, 1381
Eco47I GGWCC 1 cut(s) 1060
Eco47III AGCGCT 1 cut(s) 998
Eco57I CTGAAG 3 cut(s) 939, 1265, 1826
EcoRI GAATTC 2 cut(s) 243, 1168
EcoRII CCWGG 8 cut(s) 48, 248, 345, 398, 1062, 1636, 1899, 2171
EcoRV GATATC 3 cut(s) 337, 859, 1381
EcoT14I CCWWGG 3 cut(s) 903, 1746, 1931
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 2158
ErhI CCWWGG 3 cut(s) 903, 1746, 1931
FalI AAGNNNNNCTT 2 cut(s) 1499, 1531
FaqI GGGAC 1 cut(s) 1465
FauI CCCGC 1 cut(s) 1557
FauNDI CATATG 1 cut(s) 1164
FbaI TGATCA 4 cut(s) 121, 379, 1036, 1231
Fnu4HI GCNGC 6 cut(s) 215, 1513, 1755, 1963, 2169, 2223
FokI GGATG 6 cut(s) 449, 965, 1221, 1581, 1763, 1948
FriOI GRGCYC 1 cut(s) 2158
Fsp4HI GCNGC 6 cut(s) 215, 1513, 1755, 1963, 2169, 2223
FspBI CTAG 5 cut(s) 111, 1415, 1649, 2196, 2226
GlaI GCGC 1 cut(s) 998
GluI GCNGC 6 cut(s) 215, 1513, 1755, 1963, 2169, 2223
GsuI CTGGAG 3 cut(s) 816, 1926, 2049
HaeII RGCGCY 1 cut(s) 1000
HapII CCGG 2 cut(s) 84, 692
HhaI GCGC 1 cut(s) 999
Hin6I GCGC 1 cut(s) 997
HinP1I GCGC 1 cut(s) 997
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 7 cut(s) 80, 225, 271, 293, 494, 1301, 2020
HpaII CCGG 2 cut(s) 84, 692
HphI GGTGA 3 cut(s) 335, 551, 1877
Hpy188III TCNNGA 8 cut(s) 586, 878, 986, 1025, 1415, 1718, 1943, 2123
Hpy99I CGWCG 1 cut(s) 1728
HpyAV CCTTC 9 cut(s) 401, 720, 914, 943, 1022, 1089, 1240, 1310, 1371
HpyCH4III ACNGT 6 cut(s) 427, 448, 1456, 1548, 1657, 2117
HpyCH4IV ACGT 1 cut(s) 1918
HpyF10VI GCNNNNNNNGC 5 cut(s) 211, 556, 1633, 1751, 2143
HpyF3I CTNAG 8 cut(s) 219, 306, 516, 993, 1173, 1662, 1685, 1968
HpySE526I ACGT 1 cut(s) 1918
HspAI GCGC 1 cut(s) 997
KpnI GGTACC 1 cut(s) 199
Ksp22I TGATCA 4 cut(s) 121, 379, 1036, 1231
LmnI GCTCC 9 cut(s) 157, 253, 929, 956, 1945, 2035, 2060, 2143, 2161
Lsp1109I GCAGC 6 cut(s) 226, 1499, 1766, 1974, 2155, 2234
LweI GCATC 5 cut(s) 35, 427, 520, 746, 1741
MaeI CTAG 5 cut(s) 111, 1415, 1649, 2196, 2226
MaeII ACGT 1 cut(s) 1918
MaeIII GTNAC 4 cut(s) 1657, 1811, 2111, 2246
MboII GAAGA 7 cut(s) 32, 875, 1112, 1115, 1118, 1202, 1421
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 5 cut(s) 349, 781, 1066, 1411, 1837
MhlI GDGCHC 3 cut(s) 307, 814, 2158
MlsI TGGCCA 1 cut(s) 642
MluNI TGGCCA 1 cut(s) 642
Mox20I TGGCCA 1 cut(s) 642
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 2 cut(s) 243, 870
MscI TGGCCA 1 cut(s) 642
MseI TTAA 9 cut(s) 366, 545, 606, 756, 971, 1355, 1494, 1736, 1887
MslI CAYNNNNRTG 4 cut(s) 381, 1599, 1930, 2260
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 1340
MspI CCGG 2 cut(s) 84, 692
MspR9I CCNGG 9 cut(s) 50, 85, 250, 347, 400, 1064, 1638, 1901, 2173
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 8 cut(s) 50, 250, 347, 400, 1064, 1638, 1901, 2173
MvnI CGCG 1 cut(s) 587
MwoI GCNNNNNNNGC 5 cut(s) 211, 556, 1633, 1751, 2143
NciI CCSGG 1 cut(s) 85
NcoI CCATGG 2 cut(s) 1746, 1931
NdeI CATATG 1 cut(s) 1164
NheI GCTAGC 1 cut(s) 2225
NlaIV GGNNCC 9 cut(s) 54, 197, 351, 783, 925, 1444, 1941, 2037, 2157
NmeAIII GCCGAG 1 cut(s) 790
NmuCI GTSAC 2 cut(s) 1811, 2246
NruI TCGCGA 1 cut(s) 587
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 2 cut(s) 1694, 2053
NspV TTCGAA 1 cut(s) 1702
PagI TCATGA 1 cut(s) 985
PasI CCCWGGG 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 874, 1729
PctI GAATGC 1 cut(s) 517
PdmI GAANNNNTTC 2 cut(s) 243, 870
PfeI GAWTC 7 cut(s) 80, 225, 271, 293, 494, 1301, 2020
PflMI CCANNNNNTGG 1 cut(s) 1018
PfoI TCCNGGA 1 cut(s) 83
PkrI GCNGC 6 cut(s) 216, 1514, 1756, 1964, 2170, 2224
Ple19I CGATCG 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 1919
PshBI ATTAAT 1 cut(s) 1736
Psp6I CCWGG 8 cut(s) 48, 248, 345, 398, 1062, 1636, 1899, 2171
PspGI CCWGG 8 cut(s) 48, 248, 345, 398, 1062, 1636, 1899, 2171
PspN4I GGNNCC 9 cut(s) 54, 197, 351, 783, 925, 1444, 1941, 2037, 2157
PspPI GGNCC 4 cut(s) 805, 1060, 1159, 1634
PsrI GAACNNNNNNTAC 2 cut(s) 1636, 1668
PstI CTGCAG 2 cut(s) 474, 948
PsuI RGATCY 5 cut(s) 349, 781, 1066, 1411, 1837
PvuI CGATCG 1 cut(s) 278
RruI TCGCGA 1 cut(s) 587
RsaI GTAC 7 cut(s) 47, 197, 479, 1129, 1387, 1667, 1917
RsaNI GTAC 7 cut(s) 46, 196, 478, 1128, 1386, 1666, 1916
RseI CAYNNNNRTG 4 cut(s) 381, 1599, 1930, 2260
SaqAI TTAA 9 cut(s) 366, 545, 606, 756, 971, 1355, 1494, 1736, 1887
SatI GCNGC 6 cut(s) 215, 1513, 1755, 1963, 2169, 2223
Sau96I GGNCC 4 cut(s) 805, 1060, 1159, 1634
ScrFI CCNGG 9 cut(s) 50, 85, 250, 347, 400, 1064, 1638, 1901, 2173
SduI GDGCHC 3 cut(s) 307, 814, 2158
SfaNI GCATC 5 cut(s) 35, 427, 520, 746, 1741
SfcI CTRYAG 3 cut(s) 470, 944, 1551
SfuI TTCGAA 1 cut(s) 1702
SinI GGWCC 1 cut(s) 1060
SmiMI CAYNNNNRTG 4 cut(s) 381, 1599, 1930, 2260
SmlI CTYRAG 2 cut(s) 200, 1023
SmoI CTYRAG 2 cut(s) 200, 1023
SsiI CCGC 4 cut(s) 1238, 1338, 1564, 2073
SspI AATATT 2 cut(s) 1772, 1789
SspMI CTAG 5 cut(s) 111, 1415, 1649, 2196, 2226
StyD4I CCNGG 9 cut(s) 48, 83, 248, 345, 398, 1062, 1636, 1899, 2171
StyI CCWWGG 3 cut(s) 903, 1746, 1931
TaaI ACNGT 6 cut(s) 427, 448, 1456, 1548, 1657, 2117
TaiI ACGT 1 cut(s) 1921
TaqI TCGA 3 cut(s) 274, 861, 1702
TatI WGTACW 2 cut(s) 477, 1665
TfiI GAWTC 7 cut(s) 80, 225, 271, 293, 494, 1301, 2020
Tru1I TTAA 9 cut(s) 366, 545, 606, 756, 971, 1355, 1494, 1736, 1887
Tru9I TTAA 9 cut(s) 366, 545, 606, 756, 971, 1355, 1494, 1736, 1887
TscAI CASTG 5 cut(s) 778, 840, 1371, 1525, 2153
TseFI GTSAC 2 cut(s) 1811, 2246
TseI GCWGC 6 cut(s) 214, 1512, 1754, 1962, 2168, 2222
Tsp45I GTSAC 2 cut(s) 1811, 2246
TspDTI ATGAA 8 cut(s) 17, 51, 60, 524, 1181, 1422, 1439, 1784
TspRI CASTG 5 cut(s) 778, 840, 1371, 1525, 2153
Van91I CCANNNNNTGG 1 cut(s) 1018
VneI GTGCAC 1 cut(s) 810
VpaK11BI GGWCC 1 cut(s) 1060
VspI ATTAAT 1 cut(s) 1736
XapI RAATTY 2 cut(s) 243, 1168
XbaI TCTAGA 1 cut(s) 1414
XceI RCATGY 2 cut(s) 1694, 2053
XmnI GAANNNNTTC 2 cut(s) 243, 870
XspI CTAG 5 cut(s) 111, 1415, 1649, 2196, 2226
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.