MD05G1229400.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004982
Physical Location & Seq
Forward (+)
36288355 .. 36294765
6411 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1229400.v1.1.491

Sequence Viewer

Length: 3639 bp
ATGGAACCTGGGAGGGATGACTACCGACCTGTATCCCAGTCATACATGCCGGACTCTCTGGGCAGCATGCATACTAATATGAGATCTAATGACATTAAACCAGAAGTTAAACCTGCACATAATTACTCCATACAGACCGGAGAGGAGTTTGCTCTTCAATTTATGCTTGACCGGGTGAACCCCAGAATTCCTGTACATCCAAATGCTGGTGGCGATCCAAATTATGCAACAAACTATGTTGAACTGAAAGGAATTTTAGGAATCAGTCATACAGGCTCTGAGAGTGGTTCAGATGCTTCAATGCTTCCTAATGCTGAAAGGGGTCCAAAACAGTTTGAACAAAAGAGCTCAGCTTTACATGATGATAGAAATAACCATGCATCTGTTCAATCAGTACCACGAGCTTTATCAGGCTATGAAAATAGTCATATGCGTGGATATGCCTCTTATGCAGCCTCTGATAGCTCATCAACGAAAATGAAGGTTCTCTGTAGCTTTGGTGGTAAAATCTTACCCCGACCGAGCGATGGGAAGCTCAGGTATGTTGGAGGCGAAACACGCATTATCCGTGTTAGAAAGGACATTTCCTGGCAGGAGCTTATACATAAAGCTTTATCAATTTATAACCAAGTGCATGTAATTAAATATCAGCTTCCTGGAGAGGAACTTGATGCCTTAGTTTCTGTATCTTGTGACGAGGATCTGCAGAATATGATGGAAGAATGGAATGAAGTAGAAGATAAAGAAGGACCACAAAAGCTTAGGATGTTTTTGTTCTCCATGAGTGATTTGGAGGAAGCTCAGTTTGGTCTGCACAGTGCGGATGGTGATTCTGAGGTTCAGTATGTAGTTGCTGTCAATGGAATGGACCTGGGGTCAAGAAAAAATTCGACTCTACATGGTTTGACGACCACTTTAGCAAATAATTTAGATGAGCTCAATGGACAACATATTGAGAAGGAGACAAGTAGGGTTGCAAAAAACTCAGTAGGGTTAGGCACTTTGAATTCAACTGCCAATATTGTTTCATTGAGAACTGTTCAATCTTCCGAGCCAATCCTGCCTAACCCTTCAAATGCCTTTGAAGCTTATCCTCCTTTTCAGCATACGGACGTGATGCATTATGGACAAAATATGCAGTACCCATTGCACAATGGCCATGCCCTTCCAAGTCACTCCCCATTTGGAGGAACTGTATCGGTGTCTAATCATGGGATTTTAAATCTACAAGGAGGTTCAATTGAAGGCCAGCCATCCAGTGGCTCAAGAGAACAGAACTTCGAGATGCCAGTAAAGCAGGTGAAACCCAAATACGATGATTTACTGCAGCAAGAGAGTGACCCTGAAAAACTCCGTCCCTCAGGAGTAGAGGACTCTGTTCCTTTACAGCCATATGATGGAAGTTTGATGAACTATCATGCTGTCAAAGAAGCATCAAAAGATGAAAGAAAGTACCAGGAACCTGAAAAGGTTGCTTCGTCAATTGATCCAGGGAACCCAATTCTGGTGCATAAATCTAGTGAAGTTGAGGATAGTTTCACAACTAGCAATGCATTTGCTCCTGCTTATGCTGACCATTTGTCCAATGGAATTGATTCGGGTTGCCACGAGCTACCTGTGCTTCCTAAGAGAGTCTACCACTCAGAAAGAATTCCCAGGGAGCAGGCAGAGTTGCTGAATCGATCAACAAAGTCTGACGATTCTCATGGTCCTCCGTTTCCCGTTACTCTTTCACGTTCTGATATCACCAGGCAGGATCCAGTCATGGAAGGAGTTAACAAATTACAGGAACATGGCAACCTTACTCCTCCGACTGAACAATCTACCCCAACAACATATATCGATGCTCAAACTGTTGATGATGGACTCGTCCAACTTCAAAAGTACAAGGAAGTTGCTGATTCAGTTTCTCAGATGAATGCAAAGCTTTTGCAAGATGTAGATGGTGAAGTAAAGCGGGCATTGCCAAACCATATGGTTGATAAAGTTGCAGAAGCTGGATCTGAGTTTCCAGATGTAAGTCGGCTACCTTCTGGAAAGCAACATGAGGTATCTGCATCTAATCATTCTGAGGTTAATCAGAAAGAGGACACCAGCAAGGATCCAAGGGCTGTAGACACTATGGGACATGCAGAGCTCACTTCTTTGACAGGGAAGTTATCTAAAGATGCTTCTCAAGAAACAGCTTCAGTTGGTGTTTCTACCCCAGTGCAGGGAGACATCATTATTGATATTGAAGAACGATTTCCCAGAGATTTCCTGTCAGATATATTCTCCAAGGCAATACTTTCTGAAGACTCCCCTGATATTGGTTTGTTGCAAAAGGATGGAACTGGTTTGAGCTTAAATATGGAAAATCATGAACCAAGACGCTGGTCATATTTCCAGAAGTTAGCACAAGAAGGGTTTGATAAAAAAGATGTTTCTCTTATGGACCAGGATCTTGGTTTTCCGCCTGTGATTGGAAATGAAGAAGGTAGATCTTATCATGCGACACCGTTAACAGGAGAAGGAGCTGGTCCTCAGCCTAAATTTGTGGAAGACATGCGCACAGAGTTGCCTGGAATGGCTAAAGCTAATGCAACAGCTCTGCACTCTAATTATGGTGATTCCCAAGTGAAGGACACTGAAAGTATGCAATTTGAGGGAATGATGGAAAACGTAAGAGCGCAGGAGTTGGAGTATGAGGATGGGAAGTCTGCAAGCAGAAAAGCTGGTTTACCTCCTTTAGATCCTTCTTTGGGAGATTTTGATATCCGTACCTTGCAGTTAATAAAGAACGAAGACCTTGAACAGCTGAAGGAACTGGGTTCTGGTACCTTTGGGACTGTATACCATGGAAAATGGAGGGGCAGTGATGTTGCCATTAAGAGGCTAAATAAGAGCTGCTTCACTGGGAGATCGTCAGAGCAAGAGAGATTGATTATAGAGTTCTGGCGGGAAGCTGACATTCTTTCAAAGCTTCACCATCCAAATGTTGTGGCATTTTATGGTGTGGTGCAAGATGGACCAGGGGGAACACTTGCTACTGTTACGGAGTACATGGTTGATGGTTCTCTTAGGCATGTCTTACTTCGAAAAGACAGGTATCTCGATCGCCGCAAGCGACTAATAATTGCCATGGATGCAGCATTTGGAATGGAATATTTACACTCAAAGAATATTGTGCATTTTGACTTGAAGTGTGACAACTTACTCGTGAACCTAAAAGATCCTGCACGGCCAATTTGCAAGGTCGGTGATTTTGGGCTTTCAAAAATTAAACGAAATACCCTGGTTTCTGGTGGTGTACGGGGAACATTACCATGGATGGCCCCAGAGTTGCTGAATGGCAGCAGCACCAAGGTCTCCGAAAAGGTTGATATTTTCTCATTTGGCATCGTCTTGTGGGAGATTCTTACTGGCGAGGAGCCATATGCCAACATGCACTATGGAGCAATCATAGGAGGTATTGTAAATAACACGTTGAGACCCACCATTCCAAGCCATTGTGATCCCGAATGGAAAACGCTTATGGAGCAGTGTTGGGCCCCTAATCCTGCAGCCAGGCCATCTTTCACAGAAATCACCAGATGCTTACGAGTAATGACTACAGCGGCTAGCCAACCTAAAGCACAGGGTCACAAAGCTCCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1213

Amino Acids

133.99

Weight (kDa)

5.44

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 176 - 259 3e-18 PB1 domain
PK_Tyr_Ser-Thr PF07714 931 - 1193 1.2e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 931 - 1190 9.9e-49 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AarI CACCTGC 1 cut(s) 1288
Acc16I TGCGCA 1 cut(s) 2549
Acc36I ACCTGC 2 cut(s) 121, 1288
Acc65I GGTACC 1 cut(s) 2816
AccB1I GGYRCC 1 cut(s) 2816
AccB7I CCANNNNNTGG 3 cut(s) 206, 1259, 1397
AccI GTMKAC 3 cut(s) 1635, 2115, 2832
AciI CCGC 6 cut(s) 821, 1957, 2453, 2938, 3100, 3596
AcoI YGGCCR 2 cut(s) 1156, 3221
AcsI RAATTY 6 cut(s) 186, 252, 886, 1006, 1650, 2531
AcuI CTGAAG 3 cut(s) 2172, 2313, 2819
AfaI GTAC 9 cut(s) 195, 396, 1142, 1454, 1886, 2761, 2818, 3041, 3291
AflIII ACRYGT 1 cut(s) 3462
AhdI GACNNNNNGTC 2 cut(s) 874, 1579
AjiI CACGTC 1 cut(s) 1114
AjuI GAANNNNNNNTTGG 2 cut(s) 789, 821
Alw21I GWGCWC 3 cut(s) 350, 939, 2139
Alw26I GTCTC 4 cut(s) 956, 2211, 3352, 3463
AlwNI CAGNNNCTG 3 cut(s) 278, 458, 1997
AoxI GGCC 6 cut(s) 1156, 1246, 3221, 3312, 3528, 3548
ApaI GGGCCC 1 cut(s) 3532
ApeKI GCWGC 8 cut(s) 63, 452, 1327, 2886, 3128, 3333, 3336, 3542
ApoI RAATTY 6 cut(s) 186, 252, 886, 1006, 1650, 2531
Asp700I GAANNNNTTC 4 cut(s) 886, 1594, 1650, 2244
Asp718I GGTACC 1 cut(s) 2816
AspLEI GCGC 2 cut(s) 2550, 2671
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 9 cut(s) 187, 839, 1312, 1738, 1958, 2618, 2957, 3251, 3559
AsuII TTCGAA 1 cut(s) 3076
AsuNHI GCTAGC 1 cut(s) 3599
AvaII GGWCC 7 cut(s) 323, 749, 868, 1709, 2434, 2519, 3008
AxyI CCTNAGG 1 cut(s) 1360
BaeGI GKGCMC 1 cut(s) 3532
BalI TGGCCA 1 cut(s) 1158
BamHI GGATCC 2 cut(s) 1756, 2101
BanI GGYRCC 1 cut(s) 2816
BanII GRGCYC 4 cut(s) 350, 939, 2139, 3532
BauI CACGAG 3 cut(s) 399, 1607, 3197
BbsI GAAGAC 3 cut(s) 2301, 2547, 2790
Bbv12I GWGCWC 3 cut(s) 350, 939, 2139
BbvCI CCTCAGC 1 cut(s) 2523
BbvI GCAGC 8 cut(s) 75, 464, 1339, 2873, 3140, 3345, 3348, 3554
BceAI ACGGC 1 cut(s) 3236
BciVI GTATCC 1 cut(s) 43
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 4 cut(s) 956, 2211, 3352, 3463
BfaI CTAG 3 cut(s) 1518, 1545, 3600
BfmI CTRYAG 6 cut(s) 490, 704, 1325, 2112, 3540, 3591
BfuAI ACCTGC 2 cut(s) 121, 1288
BfuI GTATCC 1 cut(s) 43
BglII AGATCT 2 cut(s) 83, 2480
BlpI GCTNAGC 1 cut(s) 349
Bme18I GGWCC 7 cut(s) 323, 749, 868, 1709, 2434, 2519, 3008
BmeRI GACNNNNNGTC 2 cut(s) 874, 1579
BmgBI CACGTC 1 cut(s) 1114
BmrI ACTGGG 4 cut(s) 31, 2201, 2816, 2904
BmtI GCTAGC 1 cut(s) 3603
BmuI ACTGGG 4 cut(s) 31, 2201, 2816, 2904
BoxI GACNNNNGTC 1 cut(s) 2374
BpiI GAAGAC 3 cut(s) 2301, 2547, 2790
BpmI CTGGAG 1 cut(s) 678
Bpu10I CCTNAGC 3 cut(s) 536, 761, 2523
Bpu1102I GCTNAGC 1 cut(s) 349
Bpu14I TTCGAA 1 cut(s) 3076
BpuEI CTTGAG 2 cut(s) 1249, 2160
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 2 cut(s) 1681, 1842
BsaI GGTCTC 2 cut(s) 3352, 3463
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 8 cut(s) 1179, 1209, 2666, 2696, 2839, 2869, 3438, 3468
Bse1I ACTGG 9 cut(s) 37, 1257, 1289, 1760, 2207, 2338, 2811, 2899, 3406
Bse21I CCTNAGG 1 cut(s) 1360
Bse3DI GCAATG 3 cut(s) 1145, 1555, 1961
BseCI ATCGAT 2 cut(s) 1681, 1842
BseMI GCAATG 3 cut(s) 1145, 1555, 1961
BseNI ACTGG 9 cut(s) 37, 1257, 1289, 1760, 2207, 2338, 2811, 2899, 3406
BseRI GAGGAG 3 cut(s) 158, 1797, 3422
BseSI GKGCMC 1 cut(s) 3532
BseXI GCAGC 8 cut(s) 75, 464, 1339, 2873, 3140, 3345, 3348, 3554
BsgI GTGCAG 5 cut(s) 99, 797, 2231, 2576, 3201
Bsh1285I CGRYCG 2 cut(s) 521, 3097
BshFI GGCC 6 cut(s) 1158, 1248, 3223, 3314, 3530, 3550
BshNI GGYRCC 1 cut(s) 2816
BshVI ATCGAT 2 cut(s) 1681, 1842
BsiEI CGRYCG 2 cut(s) 521, 3097
BsiHKAI GWGCWC 3 cut(s) 350, 939, 2139
BsiSI CCGG 3 cut(s) 50, 138, 172
BslFI GGGAC 3 cut(s) 1341, 2139, 2839
BsmAI GTCTC 4 cut(s) 956, 2211, 3352, 3463
BsmFI GGGAC 3 cut(s) 1341, 2139, 2839
BsmI GAATGC 1 cut(s) 1924
BsnI GGCC 6 cut(s) 1158, 1248, 3223, 3314, 3530, 3550
Bso31I GGTCTC 2 cut(s) 3352, 3463
Bsp119I TTCGAA 1 cut(s) 3076
Bsp120I GGGCCC 1 cut(s) 3528
Bsp1286I GDGCHC 4 cut(s) 350, 939, 2139, 3532
Bsp1407I TGTACA 1 cut(s) 193
Bsp1720I GCTNAGC 1 cut(s) 349
Bsp19I CCATGG 3 cut(s) 2836, 3120, 3305
BspACI CCGC 6 cut(s) 821, 1957, 2453, 2938, 3100, 3596
BspANI GGCC 6 cut(s) 1158, 1248, 3223, 3314, 3530, 3550
BspDI ATCGAT 2 cut(s) 1681, 1842
BspHI TCATGA 1 cut(s) 2359
BspMAI CTGCAG 3 cut(s) 708, 1329, 3544
BspMI ACCTGC 2 cut(s) 121, 1288
BspOI GCTAGC 1 cut(s) 3603
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 3076
BspT107I GGYRCC 1 cut(s) 2816
BspTNI GGTCTC 2 cut(s) 3352, 3463
BsrDI GCAATG 3 cut(s) 1145, 1555, 1961
BsrGI TGTACA 1 cut(s) 193
BsrI ACTGG 9 cut(s) 37, 1257, 1289, 1760, 2207, 2338, 2811, 2899, 3406
BssNAI GTATAC 1 cut(s) 2833
BssSI CACGAG 3 cut(s) 399, 1607, 3197
BssT1I CCWWGG 6 cut(s) 2105, 2277, 2836, 3120, 3305, 3342
Bst1107I GTATAC 1 cut(s) 2833
Bst2BI CACGAG 3 cut(s) 399, 1607, 3197
Bst4CI ACNGT 8 cut(s) 333, 818, 1039, 1195, 1855, 2499, 2830, 3031
Bst6I CTCTTC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 3076
BstC8I GCNNGC 7 cut(s) 68, 1250, 1665, 1959, 2704, 3104, 3601
BstDSI CCRYGG 3 cut(s) 2836, 3120, 3305
BstHHI GCGC 2 cut(s) 2550, 2671
BstMAI GTCTC 4 cut(s) 956, 2211, 3352, 3463
BstMCI CGRYCG 2 cut(s) 521, 3097
BstMWI GCNNNNNNNGC 9 cut(s) 449, 558, 1060, 1085, 1294, 1618, 1963, 3125, 3517
BstNSI RCATGY 7 cut(s) 49, 70, 638, 2132, 2548, 3068, 3427
BstPAI GACNNNNGTC 1 cut(s) 2374
BstSFI CTRYAG 6 cut(s) 490, 704, 1325, 2112, 3540, 3591
BstSLI GKGCMC 1 cut(s) 3532
BstV1I GCAGC 8 cut(s) 75, 464, 1339, 2873, 3140, 3345, 3348, 3554
BstV2I GAAGAC 3 cut(s) 2301, 2547, 2790
BstX2I RGATCY 9 cut(s) 83, 700, 1756, 2000, 2101, 2440, 2480, 2731, 3211
BstXI CCANNNNNNTGG 2 cut(s) 2373, 2444
BstYI RGATCY 9 cut(s) 83, 700, 1756, 2000, 2101, 2440, 2480, 2731, 3211
BstZ17I GTATAC 1 cut(s) 2833
Bsu15I ATCGAT 2 cut(s) 1681, 1842
Bsu36I CCTNAGG 1 cut(s) 1360
BsuI GTATCC 1 cut(s) 43
BsuRI GGCC 6 cut(s) 1158, 1248, 3223, 3314, 3530, 3550
BsuTUI ATCGAT 2 cut(s) 1681, 1842
BtgI CCRYGG 3 cut(s) 2836, 3120, 3305
BtgZI GCGATG 1 cut(s) 540
BtrI CACGTC 1 cut(s) 1114
BtsI GCAGTG 2 cut(s) 2860, 3527
BtsIMutI CAGTG 7 cut(s) 823, 1264, 2214, 2625, 2860, 2892, 3527
BveI ACCTGC 2 cut(s) 121, 1288
Cac8I GCNNGC 7 cut(s) 68, 1250, 1665, 1959, 2704, 3104, 3601
CaiI CAGNNNCTG 3 cut(s) 278, 458, 1997
CciI TCATGA 1 cut(s) 2359
CfoI GCGC 2 cut(s) 2550, 2671
ClaI ATCGAT 2 cut(s) 1681, 1842
CseI GACGC 1 cut(s) 2379
Csp6I GTAC 9 cut(s) 194, 395, 1141, 1453, 1885, 2760, 2817, 3040, 3290
CspCI CAANNNNNGTGG 2 cut(s) 2961, 2996
CviQI GTAC 9 cut(s) 194, 395, 1141, 1453, 1885, 2760, 2817, 3040, 3290
DraI TTTAAA 1 cut(s) 1221
DriI GACNNNNNGTC 2 cut(s) 874, 1579
EaeI YGGCCR 2 cut(s) 1156, 3221
Eam1104I CTCTTC 1 cut(s) 159
Eam1105I GACNNNNNGTC 2 cut(s) 874, 1579
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 2442
Ecl136II GAGCTC 3 cut(s) 348, 937, 2137
Eco130I CCWWGG 6 cut(s) 2105, 2277, 2836, 3120, 3305, 3342
Eco24I GRGCYC 4 cut(s) 350, 939, 2139, 3532
Eco31I GGTCTC 2 cut(s) 3352, 3463
Eco32I GATATC 2 cut(s) 1744, 2755
Eco47I GGWCC 7 cut(s) 323, 749, 868, 1709, 2434, 2519, 3008
Eco53kI GAGCTC 3 cut(s) 348, 937, 2137
Eco57I CTGAAG 3 cut(s) 2172, 2313, 2819
Eco81I CCTNAGG 1 cut(s) 1360
EcoICRI GAGCTC 3 cut(s) 348, 937, 2137
EcoO109I RGGNCCY 1 cut(s) 3529
EcoRI GAATTC 3 cut(s) 186, 1006, 1650
EcoRV GATATC 2 cut(s) 1744, 2755
EcoT14I CCWWGG 6 cut(s) 2105, 2277, 2836, 3120, 3305, 3342
EcoT22I ATGCAT 4 cut(s) 72, 382, 1122, 1555
EcoT38I GRGCYC 4 cut(s) 350, 939, 2139, 3532
ErhI CCWWGG 6 cut(s) 2105, 2277, 2836, 3120, 3305, 3342
FalI AAGNNNNNCTT 2 cut(s) 2873, 2905
FaqI GGGAC 3 cut(s) 1341, 2139, 2839
FauI CCCGC 2 cut(s) 1950, 2931
FauNDI CATATG 4 cut(s) 429, 1393, 1974, 3415
FblI GTMKAC 3 cut(s) 1635, 2115, 2832
FriOI GRGCYC 4 cut(s) 350, 939, 2139, 3532
FspAI RTGCGCAY 1 cut(s) 2549
FspBI CTAG 3 cut(s) 1518, 1545, 3600
FspI TGCGCA 1 cut(s) 2549
GlaI GCGC 2 cut(s) 2549, 2670
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 6 cut(s) 1158, 1248, 3223, 3314, 3530, 3550
HapII CCGG 3 cut(s) 50, 138, 172
HgaI GACGC 1 cut(s) 2379
HhaI GCGC 2 cut(s) 2550, 2671
Hin6I GCGC 2 cut(s) 2548, 2669
HinP1I GCGC 2 cut(s) 2548, 2669
HincII GTYRAC 2 cut(s) 1777, 2502
HindII GTYRAC 2 cut(s) 1777, 2502
HindIII AAGCTT 5 cut(s) 609, 758, 1086, 1925, 2960
HpaI GTTAAC 2 cut(s) 1777, 2502
HpaII CCGG 3 cut(s) 50, 138, 172
HphI GGTGA 9 cut(s) 187, 839, 1312, 1738, 1958, 2618, 2957, 3251, 3559
Hpy166II GTNNAC 9 cut(s) 178, 1636, 1777, 2116, 2502, 2720, 2833, 3202, 3290
Hpy8I GTNNAC 9 cut(s) 178, 1636, 1777, 2116, 2502, 2720, 2833, 3202, 3290
HpyCH4III ACNGT 8 cut(s) 333, 818, 1039, 1195, 1855, 2499, 2830, 3031
HpyCH4IV ACGT 4 cut(s) 1113, 1735, 2661, 3464
HpyF10VI GCNNNNNNNGC 9 cut(s) 449, 558, 1060, 1085, 1294, 1618, 1963, 3125, 3517
HpySE526I ACGT 4 cut(s) 1113, 1735, 2661, 3464
HspAI GCGC 2 cut(s) 2548, 2669
KpnI GGTACC 1 cut(s) 2820
KspAI GTTAAC 2 cut(s) 1777, 2502
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 8 cut(s) 595, 1564, 1660, 2513, 3409, 3434, 3517, 3634
Lsp1109I GCAGC 8 cut(s) 75, 464, 1339, 2873, 3140, 3345, 3348, 3554
MaeI CTAG 3 cut(s) 1518, 1545, 3600
MaeII ACGT 4 cut(s) 1113, 1735, 2661, 3464
MaeIII GTNAC 7 cut(s) 692, 1172, 1337, 1723, 3031, 3185, 3620
MboII GAAGA 9 cut(s) 146, 731, 749, 1038, 2249, 2306, 2483, 2552, 2795
MfeI CAATTG 2 cut(s) 1239, 1482
MflI RGATCY 9 cut(s) 83, 700, 1756, 2000, 2101, 2440, 2480, 2731, 3211
MhlI GDGCHC 4 cut(s) 350, 939, 2139, 3532
MlsI TGGCCA 1 cut(s) 1158
MluNI TGGCCA 1 cut(s) 1158
MlyI GAGTC 6 cut(s) 47, 886, 1367, 1641, 1860, 2291
MmeI TCCRAC 4 cut(s) 526, 1835, 1897, 2658
Mox20I TGGCCA 1 cut(s) 1158
Mph1103I ATGCAT 4 cut(s) 72, 382, 1122, 1555
MroXI GAANNNNTTC 4 cut(s) 886, 1594, 1650, 2244
MscI TGGCCA 1 cut(s) 1158
MslI CAYNNNNRTG 4 cut(s) 201, 432, 2973, 3304
Msp20I TGGCCA 1 cut(s) 1158
MspA1I CMGCKG 2 cut(s) 2797, 3596
MspI CCGG 3 cut(s) 50, 138, 172
MunI CAATTG 2 cut(s) 1239, 1482
Mva1269I GAATGC 1 cut(s) 1924
MwoI GCNNNNNNNGC 9 cut(s) 449, 558, 1060, 1085, 1294, 1618, 1963, 3125, 3517
NciI CCSGG 1 cut(s) 173
NcoI CCATGG 3 cut(s) 2836, 3120, 3305
NdeI CATATG 4 cut(s) 429, 1393, 1974, 3415
NheI GCTAGC 1 cut(s) 3599
NmuCI GTSAC 5 cut(s) 692, 1172, 1337, 3185, 3620
NsbI TGCGCA 1 cut(s) 2549
NsiI ATGCAT 4 cut(s) 72, 382, 1122, 1555
NspI RCATGY 7 cut(s) 49, 70, 638, 2132, 2548, 3068, 3427
NspV TTCGAA 1 cut(s) 3076
PaeI GCATGC 1 cut(s) 70
PagI TCATGA 1 cut(s) 2359
PaqCI CACCTGC 1 cut(s) 1288
PasI CCCWGGG 1 cut(s) 1656
PciSI GCTCTTC 1 cut(s) 159
PcsI WCGNNNNNNNCGW 2 cut(s) 565, 3103
PctI GAATGC 1 cut(s) 1924
PdmI GAANNNNTTC 4 cut(s) 886, 1594, 1650, 2244
PfeI GAWTC 8 cut(s) 261, 830, 1595, 1678, 1700, 1901, 2609, 3394
PflMI CCANNNNNTGG 3 cut(s) 206, 1259, 1397
PfoI TCCNGGA 1 cut(s) 655
Ple19I CGATCG 1 cut(s) 3097
PleI GAGTC 6 cut(s) 47, 886, 1367, 1640, 1860, 2291
PpsI GAGTC 6 cut(s) 47, 886, 1367, 1640, 1860, 2291
PshAI GACNNNNGTC 1 cut(s) 2374
PsiI TTATAA 1 cut(s) 624
Psp124BI GAGCTC 3 cut(s) 350, 939, 2139
PspOMI GGGCCC 1 cut(s) 3528
PsrI GAACNNNNNNTAC 2 cut(s) 3010, 3042
PstI CTGCAG 3 cut(s) 708, 1329, 3544
PstNI CAGNNNCTG 3 cut(s) 278, 458, 1997
PsuI RGATCY 9 cut(s) 83, 700, 1756, 2000, 2101, 2440, 2480, 2731, 3211
PvuI CGATCG 1 cut(s) 3097
PvuII CAGCTG 1 cut(s) 2797
RsaI GTAC 9 cut(s) 195, 396, 1142, 1454, 1886, 2761, 2818, 3041, 3291
RsaNI GTAC 9 cut(s) 194, 395, 1141, 1453, 1885, 2760, 2817, 3040, 3290
RseI CAYNNNNRTG 4 cut(s) 201, 432, 2973, 3304
SacI GAGCTC 3 cut(s) 350, 939, 2139
SapI GCTCTTC 1 cut(s) 159
SchI GAGTC 6 cut(s) 47, 886, 1367, 1641, 1860, 2291
SduI GDGCHC 4 cut(s) 350, 939, 2139, 3532
SfcI CTRYAG 6 cut(s) 490, 704, 1325, 2112, 3540, 3591
SfuI TTCGAA 1 cut(s) 3076
SinI GGWCC 7 cut(s) 323, 749, 868, 1709, 2434, 2519, 3008
SmiMI CAYNNNNRTG 4 cut(s) 201, 432, 2973, 3304
SmlI CTYRAG 2 cut(s) 1264, 2175
SmoI CTYRAG 2 cut(s) 1264, 2175
SphI GCATGC 1 cut(s) 70
SsiI CCGC 6 cut(s) 821, 1957, 2453, 2938, 3100, 3596
SspI AATATT 3 cut(s) 1021, 3146, 3163
SspMI CTAG 3 cut(s) 1518, 1545, 3600
SstI GAGCTC 3 cut(s) 350, 939, 2139
StyI CCWWGG 6 cut(s) 2105, 2277, 2836, 3120, 3305, 3342
TaaI ACNGT 8 cut(s) 333, 818, 1039, 1195, 1855, 2499, 2830, 3031
TaiI ACGT 4 cut(s) 1116, 1738, 2664, 3467
TaqI TCGA 6 cut(s) 890, 1281, 1681, 1842, 3076, 3093
TaqII GACCGA 1 cut(s) 535
TatI WGTACW 3 cut(s) 193, 1884, 3039
TauI GCSGC 2 cut(s) 3102, 3599
TfiI GAWTC 8 cut(s) 261, 830, 1595, 1678, 1700, 1901, 2609, 3394
TscAI CASTG 7 cut(s) 823, 1264, 2214, 2632, 2860, 2899, 3527
TseFI GTSAC 5 cut(s) 692, 1172, 1337, 3185, 3620
TseI GCWGC 8 cut(s) 63, 452, 1327, 2886, 3128, 3333, 3336, 3542
Tsp45I GTSAC 5 cut(s) 692, 1172, 1337, 3185, 3620
TspDTI ATGAA 9 cut(s) 432, 494, 744, 1017, 1424, 1458, 1931, 2376, 2484
TspGWI ACGGA 6 cut(s) 557, 1124, 1343, 1704, 2747, 3050
TspRI CASTG 7 cut(s) 823, 1264, 2214, 2632, 2860, 2899, 3527
Van91I CCANNNNNTGG 3 cut(s) 206, 1259, 1397
VpaK11BI GGWCC 7 cut(s) 323, 749, 868, 1709, 2434, 2519, 3008
XapI RAATTY 6 cut(s) 186, 252, 886, 1006, 1650, 2531
XceI RCATGY 7 cut(s) 49, 70, 638, 2132, 2548, 3068, 3427
XcmI CCANNNNNNNNNTGG 3 cut(s) 787, 1256, 1583
XmiI GTMKAC 3 cut(s) 1635, 2115, 2832
XmnI GAANNNNTTC 4 cut(s) 886, 1594, 1650, 2244
XspI CTAG 3 cut(s) 1518, 1545, 3600
Zsp2I ATGCAT 4 cut(s) 72, 382, 1122, 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.