MD10G1208700.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004982
Physical Location & Seq
Forward (+)
30795594 .. 30802567
6974 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1208700.v1.1.491

Sequence Viewer

Length: 3681 bp
ATGCCTGAACAATTAAGAACGCATAAGCAGTTCCAAGACAATTCCATGGAACCTGGGAGGGATGATTACGAACCTGCATCCCAGTCATACATGCCAGACTCTCTGAGCAGCATGCATAGTGATATGAGATCTAATGACTTTATACCAGAAGTTAAACCTGCACATAATTTCTCCATACAGACCGGAGAGGAGTTTTCTCTTCAATTTATGCTTGATCGGGTGAACCACAGGATTCCTTTGCATCCAAATGCTGTTGGCGATCCAAATTATGCAACAAATTATGTTGAACTGAAAGGAATTTTAGGCATCAGTCATACAGGATCTGAGAGTGGGTCAGATACTTCGATGCTTCATATTGCTGACAGGGGTCCAAAACAGTTTGAAAGAAAGAGCTCAGCTTTATATGACAACAGAAATAAGTATGCGTCAGTTCAATCAGTACCACGAGCTTTATCTGGCTATGGAAATAGTCATGTGCATGGATATGCCTCTTATGCAGCCTCTGATAGCTCATCAATGAAAATGAAGGTTCTCTGTAGCTTTGGTGGTAAAATCTTACCCCGACCAAGCGATGGGAAGCTAAGGTATGTTGGAGGCGAAACACGCATTATCCGTGTAAGAAAGGACATTTCCTGGCAGGAGCTTATACATAAAGCTTTGTCAATTTATAACCAAGTGCATGTAATTAAATACCAGCTTCCTGGAGAGGAACTTGATGCTTTAGTTTCTGTATCTTGTGACGAGGATCTGCAGAATATGATGGAAGAATGGAATGAAGTAGAAGATAAAGAAGGACCACAAAAGCTTAGGATGTTTTTGTTCTCCATGAGTGATTTGGAGGATGCTCAGTTTGGTCTGCACAGTGTGGATGGTGATTCTGAGGTTCAGTATGTAGTTGCTATCAATGGAATGGACCTGGGGTCAAGAAAAAATTTGGCATTACATGGTTTGACGAGCACTCTAGCAAATAATTTAGATGAGTTCAATGGACAACATATTGAGAAGGAGACAAGTAGGGTTGCAAAAGACTCGATAGGGGTAGGCTCTTTGAATTCAACTGCTAATTTTGTTACATCGAGAACTGTTCAATCTTCCGAGCCAATCCTGCCTAACTCTTCAAATGCCTATGAAACCTATCCTCCTTTTCAGCATACCCAGGTGATGCATATTGGACAAAATATGCAGCACCCATTGCACAATGGCCATGCCCTTCCAAGTCACTCCCCATTTGGAGGAACTGTTTCAGTGTCTCATCATGGGATTCTGAATCCACAAGGAGGTTCAATTGAAGGGCAGCCATCCAGTGGCTCAAGAGAACAGAATTTCGAGATGCCAGTAAAGGAGGTGAAACCCAAACGTGATGGTTTACTGCAGCCAGAGAGTGACCCTGAAAAACTCCGCCCCTCAGGGGTAGAGAACTCTGTTCCTTTACAGCCACATGATGGAAATTTGATGAACTATCTTCCTGTTGAAGAAGCATCAAAAGATGAAAGAAAGTGCCAGGAACCTGAAAAGGTTGCTTCATCAATTGATTCAGGGAACCCAATGCTGGTGCAAAAATCTAGTGAAGTTGAGGATAGCTTCACAGCTAGCAATGCATTTGCTCCTGCTTGTACTGACCATTTGTCCAATGGAGTTGATTCGGGTTACCATGAGCTACCTGTGCTTCCTAAGAGAGTCTACTACTCAGAAAGAATTCCCAGGGAGCAGGCAGAGTTGCTGAATCGATCAACAAAGTCAGATGATTCTCATGGTTCTCCGTTTCTCGTTACTCATTCACGTTCTGATATCACCCAGCAGGATTCAGTCATGGAAGGAGTTAACAAATTACAGGAACATGGCAACCTTGCTCCCCCGACTGAACAATCTACCCCAACAACAGGTACCGATGCTCAAACCGTTGATGATGGATTCATCCAACCTCAAAAGTACAAAGAATTTGCTGATTCAGTTTCTCAGATGAATGCAAAGCTTTTGCAAGATGTAGATGGTGAAGTAAAGCGGGCATTGCCAAACCATATGGTTGATAACATTGCAGAAGCTGGATCTGAGTTTCCAGATATAAGTCAGCTACCTTCTGGAAAGCAACATGAGGTATCTGCATCTAATCATTCGGAGGTTAATCAGAAAGAGGACACCAGCAAGGATCCAAGGACTGTTGACACTATGGGACATGCAGAGCCCAATTCTTTGACAGGGAAGTTTTCTAAAGATGTTTCTCAAGCAGCAGCTTCAGTTGGTGTTTCTACCCCAGTGCAGGGAGACATCATTATTGATATTGAAGAACGATTTCCCCGTGATTTCCTGTCAGATATATTCTCCAAGGCAATACTTTCCGAAGACTCCCCTGATATTGGTTTGCTGCAAAAGGATGGAACTGGTTTGAGCTTAAATATGAAAAATCATGAACCAAGACACTGGTCATATTTCCAGAAGTTGGCACAAGAAGGGTTTGATAAAAAAGATGTTTCTCTTATGGACCAGGATCTTGGTTTTCCGCCTGTGATTGGAAATGAGGACAGTAGATCTTATCATGTTACACCGTTAACAGCAGAAGGAGCTGGTTCTCAGCCTAAATTTGCAGAAGACATGCACACAGAGTTGCCTGGAATGGCTAAAGCTAACGCAACAGCTCTGCACTCTAATTATGGTCATTCCCAAGTGAAGGACACTGAAAGTATGCAATTTGAGGGAATGATGGAAAACATAAGAGCGCAAGAGTCGGAGTATGAGGATGGGAAGTCTGCAAGCAGAAGAGCTGGTTTACCTCCTTTAGATCCTTCTCTGGGAGATTTTGATATCAGTACCTTGCAGTTAATAAAGAACGAAGATCTCGAACAGCTGAAGGAACTGGGTTCTGGTACCTTTGGGACTGTGTACCATGGAAAATGGAGGGGCAGTGATGTTGCCATTAAGAGACTAAATAAGGGCTGCTTCACCGGGAGATCATCAGAGCAAGAGAGATTGACTATAGAGTTCTGGCGGGAAGCTGACATTCTTTCAAAGCTTCACCATCCAAATGTTGTGGCGTTTTATGGTGTGGTGCAAGATGGACCAGGGGGAACACTTGCTACTGTTACGGAGTACATGGTTGATGGTTCTCTTAGGCATGTCTTACTTCGAAAAGACAGGTATCTCGATCGCCGCAAGCGACTAATAATTGCCATGGATGCAGCATTTGGAATGGAATATTTACACTCAAAGAATATTGTGCATTTTGACTTGAAGTGTGACAACTTACTTGTGAACCTAAAAGATCCTGCGCGTCCAATTTGCAAGGTCGGTGATTTTGGGCTTTCAAAAATTAAGCGAAATACCCTGGTTTCTGGTGGTGTACGGGGAACGTTACCATGGATGGCCCCAGAGTTGCTGAATGGCAGCAGCACGAAGGTCTCCGAAAAGGTTGATATTTTCTCCTTTGGCATTGTCTTATGGGAGATTCTCACTGGCGAGGAGCCGTACGCCAACATGCACTATGGAGCAATTATAGGAGGCATTGTAAATAACACATTGAGACCCACCATTCCAAGTTATTGTGATCCCGAATGGAAAACGCTTATGGAGCAGTGTTGGGCCCCTAATCCTGCAGCCAGGCCATCGTTCACAGAAATCGCCAGATGCTTACGAGCAATGACTACGGCTAGCCAACCTAAAGCACACGGTCACAAAGCTCCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1227

Amino Acids

135.58

Weight (kDa)

5.48

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 191 - 274 3.1e-18 PB1 domain
PK_Tyr_Ser-Thr PF07714 946 - 1208 1.6e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 946 - 1205 4.8e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
Acc36I ACCTGC 2 cut(s) 82, 166
Acc65I GGTACC 2 cut(s) 1883, 2861
AccB1I GGYRCC 2 cut(s) 1883, 2861
AccB7I CCANNNNNTGG 4 cut(s) 572, 1304, 1442, 2437
AccI GTMKAC 1 cut(s) 1680
AccII CGCG 1 cut(s) 3265
AciI CCGC 5 cut(s) 1399, 2002, 2498, 2983, 3145
AclI AACGTT 1 cut(s) 3344
AcoI YGGCCR 1 cut(s) 1201
AcsI RAATTY 8 cut(s) 297, 931, 1051, 1321, 1447, 1695, 1937, 2576
AcuI CTGAAG 2 cut(s) 2217, 2864
AdeI CACNNNGTG 1 cut(s) 865
AhdI GACNNNNNGTC 2 cut(s) 919, 1624
Alw21I GWGCWC 2 cut(s) 395, 959
Alw26I GTCTC 6 cut(s) 1001, 1254, 2256, 2911, 3397, 3508
AlwNI CAGNNNCTG 3 cut(s) 323, 503, 2042
AoxI GGCC 4 cut(s) 1201, 3357, 3573, 3593
ApaI GGGCCC 1 cut(s) 3577
ApoI RAATTY 8 cut(s) 297, 931, 1051, 1321, 1447, 1695, 1937, 2576
Asp700I GAANNNNTTC 3 cut(s) 1240, 1695, 2289
Asp718I GGTACC 2 cut(s) 1883, 2861
AspLEI GCGC 2 cut(s) 2716, 3265
AspS9I GGNCC 8 cut(s) 368, 794, 913, 2479, 3053, 3358, 3573, 3574
AsuC2I CCSGG 1 cut(s) 2941
AsuHPI GGTGA 9 cut(s) 232, 884, 1171, 1357, 1783, 2003, 2929, 3002, 3296
AsuII TTCGAA 1 cut(s) 3121
AsuNHI GCTAGC 2 cut(s) 1589, 3641
AvaII GGWCC 5 cut(s) 368, 794, 913, 2479, 3053
AxyI CCTNAGG 1 cut(s) 1405
BaeGI GKGCMC 1 cut(s) 3577
BalI TGGCCA 1 cut(s) 1203
BamHI GGATCC 1 cut(s) 2146
BanI GGYRCC 2 cut(s) 1883, 2861
BanII GRGCYC 3 cut(s) 395, 2184, 3577
BauI CACGAG 1 cut(s) 444
BbsI GAAGAC 2 cut(s) 2346, 2592
Bbv12I GWGCWC 2 cut(s) 395, 959
BceAI ACGGC 2 cut(s) 3442, 3654
BcgI CGANNNNNNTGC 2 cut(s) 1011, 1045
BcnI CCSGG 1 cut(s) 2941
BcoDI GTCTC 6 cut(s) 1001, 1254, 2256, 2911, 3397, 3508
BfaI CTAG 4 cut(s) 962, 1563, 1590, 3642
BfmI CTRYAG 5 cut(s) 535, 749, 1370, 2970, 3585
BfuAI ACCTGC 2 cut(s) 82, 166
BglII AGATCT 3 cut(s) 128, 2525, 2830
BlpI GCTNAGC 1 cut(s) 394
Bme18I GGWCC 5 cut(s) 368, 794, 913, 2479, 3053
BmeRI GACNNNNNGTC 2 cut(s) 919, 1624
BmgT120I GGNCC 8 cut(s) 368, 794, 913, 2479, 3053, 3358, 3573, 3574
BmrI ACTGGG 3 cut(s) 76, 2246, 2861
BmtI GCTAGC 2 cut(s) 1593, 3645
BmuI ACTGGG 3 cut(s) 76, 2246, 2861
BoxI GACNNNNGTC 2 cut(s) 366, 2419
BpiI GAAGAC 2 cut(s) 2346, 2592
BpmI CTGGAG 1 cut(s) 723
Bpu10I CCTNAGC 2 cut(s) 581, 806
Bpu1102I GCTNAGC 1 cut(s) 394
Bpu14I TTCGAA 1 cut(s) 3121
BpuEI CTTGAG 2 cut(s) 1294, 2205
BpuMI CCSGG 1 cut(s) 2941
Bsa29I ATCGAT 1 cut(s) 1726
BsaI GGTCTC 2 cut(s) 3397, 3508
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 8 cut(s) 1123, 1153, 1224, 1254, 2884, 2914, 3483, 3513
Bse1I ACTGG 8 cut(s) 82, 1302, 1334, 2252, 2383, 2423, 2856, 3451
Bse21I CCTNAGG 1 cut(s) 1405
Bse3DI GCAATG 5 cut(s) 1190, 1600, 2006, 2031, 3636
BseCI ATCGAT 1 cut(s) 1726
BseMI GCAATG 5 cut(s) 1190, 1600, 2006, 2031, 3636
BseNI ACTGG 8 cut(s) 82, 1302, 1334, 2252, 2383, 2423, 2856, 3451
BseRI GAGGAG 2 cut(s) 203, 3467
BseSI GKGCMC 1 cut(s) 3577
BseYI CCCAGC 1 cut(s) 1794
BsgI GTGCAG 4 cut(s) 144, 842, 2276, 2621
Bsh1236I CGCG 1 cut(s) 3265
Bsh1285I CGRYCG 1 cut(s) 3142
BshFI GGCC 4 cut(s) 1203, 3359, 3575, 3595
BshNI GGYRCC 2 cut(s) 1883, 2861
BshVI ATCGAT 1 cut(s) 1726
BsiEI CGRYCG 1 cut(s) 3142
BsiHKAI GWGCWC 2 cut(s) 395, 959
BsiSI CCGG 2 cut(s) 183, 2940
BsiWI CGTACG 1 cut(s) 3459
BslFI GGGAC 2 cut(s) 2184, 2884
BsmAI GTCTC 6 cut(s) 1001, 1254, 2256, 2911, 3397, 3508
BsmFI GGGAC 2 cut(s) 2184, 2884
BsmI GAATGC 1 cut(s) 1969
BsnI GGCC 4 cut(s) 1203, 3359, 3575, 3595
Bso31I GGTCTC 2 cut(s) 3397, 3508
Bsp119I TTCGAA 1 cut(s) 3121
Bsp120I GGGCCC 1 cut(s) 3573
Bsp1286I GDGCHC 4 cut(s) 395, 959, 2184, 3577
Bsp1720I GCTNAGC 1 cut(s) 394
Bsp19I CCATGG 4 cut(s) 45, 2881, 3165, 3350
BspACI CCGC 5 cut(s) 1399, 2002, 2498, 2983, 3145
BspANI GGCC 4 cut(s) 1203, 3359, 3575, 3595
BspDI ATCGAT 1 cut(s) 1726
BspFNI CGCG 1 cut(s) 3265
BspHI TCATGA 1 cut(s) 2404
BspMAI CTGCAG 3 cut(s) 753, 1374, 3589
BspMI ACCTGC 2 cut(s) 82, 166
BspOI GCTAGC 2 cut(s) 1593, 3645
BspQI GCTCTTC 1 cut(s) 2749
BspT104I TTCGAA 1 cut(s) 3121
BspT107I GGYRCC 2 cut(s) 1883, 2861
BspTNI GGTCTC 2 cut(s) 3397, 3508
BsrDI GCAATG 5 cut(s) 1190, 1600, 2006, 2031, 3636
BsrI ACTGG 8 cut(s) 82, 1302, 1334, 2252, 2383, 2423, 2856, 3451
BssSI CACGAG 1 cut(s) 444
BssT1I CCWWGG 6 cut(s) 45, 2150, 2322, 2881, 3165, 3350
Bst2BI CACGAG 1 cut(s) 444
Bst6I CTCTTC 3 cut(s) 204, 1120, 2749
BstAPI GCANNNNNTGC 1 cut(s) 1192
BstBI TTCGAA 1 cut(s) 3121
BstC8I GCNNGC 7 cut(s) 113, 1591, 1710, 2004, 2749, 3149, 3643
BstDSI CCRYGG 4 cut(s) 45, 2881, 3165, 3350
BstEII GGTNACC 1 cut(s) 1646
BstFNI CGCG 1 cut(s) 3265
BstHHI GCGC 2 cut(s) 2716, 3265
BstMAI GTCTC 6 cut(s) 1001, 1254, 2256, 2911, 3397, 3508
BstMCI CGRYCG 1 cut(s) 3142
BstNSI RCATGY 7 cut(s) 94, 115, 683, 2177, 2593, 3113, 3472
BstPAI GACNNNNGTC 2 cut(s) 366, 2419
BstPI GGTNACC 1 cut(s) 1646
BstSFI CTRYAG 5 cut(s) 535, 749, 1370, 2970, 3585
BstSLI GKGCMC 1 cut(s) 3577
BstUI CGCG 1 cut(s) 3265
BstV2I GAAGAC 2 cut(s) 2346, 2592
BstXI CCANNNNNNTGG 3 cut(s) 701, 2418, 2489
Bsu15I ATCGAT 1 cut(s) 1726
Bsu36I CCTNAGG 1 cut(s) 1405
BsuRI GGCC 4 cut(s) 1203, 3359, 3575, 3595
BsuTUI ATCGAT 1 cut(s) 1726
BtgI CCRYGG 4 cut(s) 45, 2881, 3165, 3350
BtgZI GCGATG 1 cut(s) 585
BtsI GCAGTG 2 cut(s) 2905, 3572
BtsIMutI CAGTG 9 cut(s) 868, 1251, 1309, 2259, 2416, 2670, 2905, 3444, 3572
BveI ACCTGC 2 cut(s) 82, 166
Cac8I GCNNGC 7 cut(s) 113, 1591, 1710, 2004, 2749, 3149, 3643
CaiI CAGNNNCTG 3 cut(s) 323, 503, 2042
CciI TCATGA 1 cut(s) 2404
CfoI GCGC 2 cut(s) 2716, 3265
Cfr13I GGNCC 8 cut(s) 368, 794, 913, 2479, 3053, 3358, 3573, 3574
ClaI ATCGAT 1 cut(s) 1726
CseI GACGC 2 cut(s) 414, 3254
CspCI CAANNNNNGTGG 2 cut(s) 3006, 3041
DraIII CACNNNGTG 1 cut(s) 865
DriI GACNNNNNGTC 2 cut(s) 919, 1624
EaeI YGGCCR 1 cut(s) 1201
Eam1104I CTCTTC 3 cut(s) 204, 1120, 2749
Eam1105I GACNNNNNGTC 2 cut(s) 919, 1624
EarI CTCTTC 3 cut(s) 204, 1120, 2749
EciI GGCGGA 2 cut(s) 1388, 2487
Ecl136II GAGCTC 1 cut(s) 393
Eco130I CCWWGG 6 cut(s) 45, 2150, 2322, 2881, 3165, 3350
Eco24I GRGCYC 3 cut(s) 395, 2184, 3577
Eco31I GGTCTC 2 cut(s) 3397, 3508
Eco32I GATATC 2 cut(s) 1789, 2800
Eco47I GGWCC 5 cut(s) 368, 794, 913, 2479, 3053
Eco53kI GAGCTC 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 2217, 2864
Eco81I CCTNAGG 1 cut(s) 1405
Eco91I GGTNACC 1 cut(s) 1646
EcoICRI GAGCTC 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 3574
EcoO65I GGTNACC 1 cut(s) 1646
EcoRI GAATTC 2 cut(s) 1051, 1695
EcoRV GATATC 2 cut(s) 1789, 2800
EcoT14I CCWWGG 6 cut(s) 45, 2150, 2322, 2881, 3165, 3350
EcoT22I ATGCAT 3 cut(s) 117, 1167, 1600
EcoT38I GRGCYC 3 cut(s) 395, 2184, 3577
ErhI CCWWGG 6 cut(s) 45, 2150, 2322, 2881, 3165, 3350
FalI AAGNNNNNCTT 2 cut(s) 2918, 2950
FaqI GGGAC 2 cut(s) 2184, 2884
FauI CCCGC 2 cut(s) 1995, 2976
FauNDI CATATG 1 cut(s) 2019
FblI GTMKAC 1 cut(s) 1680
FriOI GRGCYC 3 cut(s) 395, 2184, 3577
FspBI CTAG 4 cut(s) 962, 1563, 1590, 3642
GlaI GCGC 2 cut(s) 2715, 3264
GsaI CCCAGC 1 cut(s) 1798
GsuI CTGGAG 1 cut(s) 723
HaeIII GGCC 4 cut(s) 1203, 3359, 3575, 3595
HapII CCGG 2 cut(s) 183, 2940
HgaI GACGC 2 cut(s) 414, 3254
HhaI GCGC 2 cut(s) 2716, 3265
Hin6I GCGC 2 cut(s) 2714, 3263
HinP1I GCGC 2 cut(s) 2714, 3263
HincII GTYRAC 3 cut(s) 1822, 2161, 2547
HindII GTYRAC 3 cut(s) 1822, 2161, 2547
HindIII AAGCTT 4 cut(s) 654, 803, 1970, 3005
HpaI GTTAAC 2 cut(s) 1822, 2547
HpaII CCGG 2 cut(s) 183, 2940
HphI GGTGA 9 cut(s) 232, 884, 1171, 1357, 1783, 2003, 2929, 3002, 3296
HpyCH4IV ACGT 3 cut(s) 1357, 1780, 3344
HpySE526I ACGT 3 cut(s) 1357, 1780, 3344
HspAI GCGC 2 cut(s) 2714, 3263
KpnI GGTACC 2 cut(s) 1887, 2865
KspAI GTTAAC 2 cut(s) 1822, 2547
LguI GCTCTTC 1 cut(s) 2749
LmnI GCTCC 9 cut(s) 640, 1609, 1705, 1855, 2558, 3454, 3479, 3562, 3676
MaeI CTAG 4 cut(s) 962, 1563, 1590, 3642
MaeII ACGT 3 cut(s) 1357, 1780, 3344
MfeI CAATTG 2 cut(s) 1284, 1527
MhlI GDGCHC 4 cut(s) 395, 959, 2184, 3577
MlsI TGGCCA 1 cut(s) 1203
MluNI TGGCCA 1 cut(s) 1203
MlyI GAGTC 5 cut(s) 92, 1022, 1686, 2336, 2729
MmeI TCCRAC 3 cut(s) 571, 1942, 2703
Mox20I TGGCCA 1 cut(s) 1203
Mph1103I ATGCAT 3 cut(s) 117, 1167, 1600
MroXI GAANNNNTTC 3 cut(s) 1240, 1695, 2289
MscI TGGCCA 1 cut(s) 1203
MslI CAYNNNNRTG 4 cut(s) 246, 477, 483, 3018
Msp20I TGGCCA 1 cut(s) 1203
MspA1I CMGCKG 1 cut(s) 2842
MspI CCGG 2 cut(s) 183, 2940
MunI CAATTG 2 cut(s) 1284, 1527
Mva1269I GAATGC 1 cut(s) 1969
MvnI CGCG 1 cut(s) 3265
NciI CCSGG 1 cut(s) 2941
NcoI CCATGG 4 cut(s) 45, 2881, 3165, 3350
NdeI CATATG 1 cut(s) 2019
NheI GCTAGC 2 cut(s) 1589, 3641
NmuCI GTSAC 5 cut(s) 737, 1217, 1382, 3230, 3662
NsiI ATGCAT 3 cut(s) 117, 1167, 1600
NspI RCATGY 7 cut(s) 94, 115, 683, 2177, 2593, 3113, 3472
NspV TTCGAA 1 cut(s) 3121
PaeI GCATGC 1 cut(s) 115
PagI TCATGA 1 cut(s) 2404
PasI CCCWGGG 1 cut(s) 1701
PciSI GCTCTTC 1 cut(s) 2749
PcsI WCGNNNNNNNCGW 4 cut(s) 610, 2293, 2832, 3148
PctI GAATGC 1 cut(s) 1969
PdmI GAANNNNTTC 3 cut(s) 1240, 1695, 2289
Pfl23II CGTACG 1 cut(s) 3459
PflMI CCANNNNNTGG 4 cut(s) 572, 1304, 1442, 2437
PfoI TCCNGGA 1 cut(s) 700
Ple19I CGATCG 1 cut(s) 3142
PleI GAGTC 5 cut(s) 92, 1022, 1685, 2336, 2728
PpsI GAGTC 5 cut(s) 92, 1022, 1685, 2336, 2728
PshAI GACNNNNGTC 2 cut(s) 366, 2419
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 395
Psp1406I AACGTT 1 cut(s) 3344
PspEI GGTNACC 1 cut(s) 1646
PspFI CCCAGC 1 cut(s) 1794
PspLI CGTACG 1 cut(s) 3459
PspOMI GGGCCC 1 cut(s) 3573
PspPI GGNCC 8 cut(s) 368, 794, 913, 2479, 3053, 3358, 3573, 3574
PsrI GAACNNNNNNTAC 2 cut(s) 3055, 3087
PstI CTGCAG 3 cut(s) 753, 1374, 3589
PstNI CAGNNNCTG 3 cut(s) 323, 503, 2042
PvuI CGATCG 1 cut(s) 3142
PvuII CAGCTG 1 cut(s) 2842
RseI CAYNNNNRTG 4 cut(s) 246, 477, 483, 3018
SacI GAGCTC 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 2749
Sau96I GGNCC 8 cut(s) 368, 794, 913, 2479, 3053, 3358, 3573, 3574
SchI GAGTC 5 cut(s) 92, 1022, 1686, 2336, 2729
SduI GDGCHC 4 cut(s) 395, 959, 2184, 3577
SfcI CTRYAG 5 cut(s) 535, 749, 1370, 2970, 3585
SfuI TTCGAA 1 cut(s) 3121
SinI GGWCC 5 cut(s) 368, 794, 913, 2479, 3053
SmiMI CAYNNNNRTG 4 cut(s) 246, 477, 483, 3018
SmlI CTYRAG 2 cut(s) 1309, 2220
SmoI CTYRAG 2 cut(s) 1309, 2220
SphI GCATGC 1 cut(s) 115
SsiI CCGC 5 cut(s) 1399, 2002, 2498, 2983, 3145
SspI AATATT 2 cut(s) 3191, 3208
SspMI CTAG 4 cut(s) 962, 1563, 1590, 3642
SstI GAGCTC 1 cut(s) 395
StyI CCWWGG 6 cut(s) 45, 2150, 2322, 2881, 3165, 3350
TaiI ACGT 3 cut(s) 1360, 1783, 3347
TaqI TCGA 8 cut(s) 344, 1031, 1076, 1326, 1726, 2835, 3121, 3138
TatI WGTACW 3 cut(s) 1613, 1929, 3084
TauI GCSGC 1 cut(s) 3147
TscAI CASTG 9 cut(s) 868, 1251, 1309, 2259, 2423, 2677, 2905, 3451, 3572
TseFI GTSAC 5 cut(s) 737, 1217, 1382, 3230, 3662
Tsp45I GTSAC 5 cut(s) 737, 1217, 1382, 3230, 3662
TspGWI ACGGA 3 cut(s) 602, 1749, 3095
TspRI CASTG 9 cut(s) 868, 1251, 1309, 2259, 2423, 2677, 2905, 3451, 3572
Van91I CCANNNNNTGG 4 cut(s) 572, 1304, 1442, 2437
VpaK11BI GGWCC 5 cut(s) 368, 794, 913, 2479, 3053
XapI RAATTY 8 cut(s) 297, 931, 1051, 1321, 1447, 1695, 1937, 2576
XceI RCATGY 7 cut(s) 94, 115, 683, 2177, 2593, 3113, 3472
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 1628
XmiI GTMKAC 1 cut(s) 1680
XmnI GAANNNNTTC 3 cut(s) 1240, 1695, 2289
XspI CTAG 4 cut(s) 962, 1563, 1590, 3642
Zsp2I ATGCAT 3 cut(s) 117, 1167, 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.