RLG00000032775

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004982
Physical Location & Seq
Forward (+)
18949097 .. 18954675
5579 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032775

Sequence Viewer

Length: 3654 bp
ATGGAACCTGGAAGGGAGGAATTCCACCCTGCATCCCAGCAATATATGCCGAACTCTCTGAGCAGCATGCATTCTGATATGAGGTCCCATAACCTTAATACTTCAGATATTAAACCTGTACATAATTATTCAATAACAGGTGAGGAGTTTTCTCTTGAATTTATGCTTGATCGAGTGAACCCCAGGAAGCCTTTGCATCCAAATGCTGTGGGTGATCCAAGTTATGTTGAACTAAAAGGAATGTTAGGAATCAGTCACGGAGGTTTTGAAAGTGGTTCAGATGCTTCAATGGTTGCTATATCTGAAAGAGGTCCAAACCAGTTTGAGAGAAACAGCTCATATTTACATGATGACAGAAATAACTATGGATCAGTTCAATCAGTACCACGAGCTTCTTCAGACTATGGAAATAGTCGTGTTCATGGATATGCCTTATCTGGAGCCTCTGATAGCTCATCAATGAGAATGAAGGTTCTTTGTAGCTTTGGTGGTAAAATTTTACCCCGACCAAGTGATGGGAAACTCAGGTATGTTGGAGGTGATACACGCATTATCCGTATTAGAAAGGACATTACCTGGCAGGAGCTTATACATAAAGCCTTATCAATTTATAACCAATTGCATATAATTAAATATCAGCTTCCAGGAGAGGAGCTTGATGCCTTGGTTTCTGTATCTTGTGATGAGGATCTGCAGAATATGATGGAGGAATGGAACGAACTAGAAGATAAAGAGGGGCCGCAGAAGCTTAGAATGTTTTTTTTCTCCATCAGTGATTTGGAGGATCAGTTTGGTCTGCACACTGTGGATGGTGACTCTGAGGTTCAGTATGTAGTTGCTGTCAATGGCATGGACCTGGGATCAAGAAAAAACTCGAGCCTACATGGTTTGACAAGCTCGGCAGCTAGTCATTTAGATGAGACAAATAGACACAATATTCAGAAGGGAACAAGTAGTGTTGCGAAAGACTCAATAGGGATAGATGCTCCAGTTTTGACTGGCAAAATCATTTCAGCGACAACTGTTCAATCTTCTGAACCTATGCTGCCAAGCTCTTCAAATGCCTATGAAGCTTATACTCATTTTCAACATGGACAGGTTATGCATTATGGACAAAATGTGCAGTACCCATTGCACAATGGCCATGCCTTTCCAAGTCAATCCCATTTTGGAGATGCTCCTATTTCAGTGCCTCATCATGGTATTATGAATCAAGGAGGTTCAATTGAAGGGCCGACCAGTGGCTTGAGAGAACGTAGTTTCGAGATGCCAGTGAAGGAGGTACAACCATTACGTGATGGTTCAGTCCAGCAAGAGAGTGACCCTGAAAAACTTCGCCCCTCAAGGAAAGAGCAATCTGTCCCCAAACCGCTGTATGATGGTAATTTGATGAATTACCCTCCTGTTGAAGAAGCATCAAAAGATGAAAGAAAGTACCAGGAACCTGAAAATGTTGCTTCATCAATTGATTCCGGGAAGTCTATGCTGGTTCTCAATCCTAGTGAAGTTGATCATCAGTCCACATCTAACAATGCATTTGCTCCTACTTATGCTGACCCAATGTCCAATGGAATTGATTTGGGGTACCTTGAGCCACCTGTGCAGCCTCAGAGAATCTACTACTCGGAAAGAATTCCCAGGGAGCAGGCAGAGTTGCTGAATCGATCGTCAAAGTCTGATGATTCGCATGGTGCTCAGTTTCTCGTATCTCATTCACGCTCTGATATCGTTCACCAGGATCCAATTGCAGGAGTTAAGAAATTGCATGATCATGCAAACCTTCCTCCCAGGTCTGAACAACAATCCTCCTCAACTGTAAATGTGGATGCTCAAACTGTTGATGATGGACTTGCTCAACTGCAGAAGTACAAAGAGTTTGCTGATTCAATTTGTGAGATGAATGCTAAGCTTTTGCAAGATGCAGATGGTGAATTAAAGCCAGCATTGCCAAACTCTGTGGATACTAAAGAGATCGCGAACAGGGATAAGATCCTTAAATCTGACCAAGAAACAAACTGCCTCAAAGGTAGCCATAAGAAACTTGTGGTTGATGACATTGCAGAAGCTATATCAGAGTATCCGGCTCTAAGTCAGATACCTTCTGTGAAGCTCCATGAGGTTTCTGCATCTAATCATTCAGAGCTTAATCAGGCCGAGGCCATTGGCAAGGAAACAAATACTGCTGACAATATGGGCCATGCACAAGTTGGTATATCTACTCCAGTGCAGGGAGACATAATTATTGATATCGAAGAACGATTTCCTCGTGATTTCCTGTCTGATATATTCTCCAAGGCAATACTTTCTGAAGGCTCCCCTGATGTTGGTTTACTGCAGAAGGATGGAGCTGGTTTGAGCTTAAATATGGAAAATCATGATCCGAAGCGCTGGTCATATTTCCAAAAGTTGGATCAAGAAGGGGCTGATCAACAAGATGTTTCTCTTATGGACCAGGATCTTGGTTTTCCTTCTGCAATTAGAAATGTTGAAGAAGAAGATAATAAATCTTATCATTGTACACCTTTACCAACAGATGGAGTTCCAATGGCCCATATGAATTCTCAGCCTAATTTTGGAGTAGATATTAGCAAAGAAACTGGACTGACTAAAGCTAATTATGATCATCCGCAACTGAAGGAAACTGAAAGTATGCAGTTTGAGGCCATGATGGAAAACTTACGAGTGCCAGAATCAGATTATGAGGAAGGAAAGTTTGCAAGTAGAACCGCTGGTTTACCTCCTTTAAATCCTTCACTGGGAGATTTTGATATCAGTACCTTGCAGTTGATAAAGAATGAAGATCTAGAACAGATGAAAGAACTGGGTTCTGGCACCTTTGGGACTGTGTATCATGGAAAATGGAGGGGGAGTGATGTTGCCATTAAAAGACTAAATAAGAGCTGCTTCACTGGGAGATCATCAGAGCAAGAGAGACTGTCTATAGAGTTCTGGCGGGAAGCTGACATTCTTTCAAAGCTACACCATCCAAATGTGGTGGCATTTTATGGTGTAGTGCAAGATGGGCCAGGGGGAACACTAGCTACCGTAACTGAGTACATGGTTGATGGTTCTCTTAGGCATGTCTTACTTCGAAAAGATAGGTATCTTGATCGTCGCAAGCGATTAATAATTGCCATGGATGCAGCATTTGGAATGGAATATTTGCACTCAAAGAATATTGTTCATTTTGACCTGAAGTGTGACAACTTACTTGTGAACCTAAAAGATCCTCAACGGCCAATTTGCAAGGTCGGTGATTTTGGCCTTTCAAAAATAAAGCGAAATACCCTGGTTTCTGGTGGTGTACGTGGAACGTTACCATGGATGGCTCCAGAGTTGCTGAATGGAAGCAGCACTAAGGTCTCTGAAAAGGTGGATATTTTCTCCTTTGGTATTGTCTTATGGGAGATTCTTACTGGAGAGGAGCCGTATGCCAACATGCACTATGGAGCAATTATAGGCGGTATTGTGAACAACACATTGAGACCCACCATTCCAAGTTACTGTGATCCAGAATGGAGAACACTTATGGAGCAGTGTTGGGCTCCTAATCCTGCTGCCCGGCCATCGTTCACAGAAATTGCTAGTTGCTTACGAGTTATGACAACAGCAGCTAGCCAAACCAAAGCACAGGGTCACAAGGCATCTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

1218

Amino Acids

134.97

Weight (kDa)

5.35

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 172 - 253 2.9e-17 PB1 domain
PK_Tyr_Ser-Thr PF07714 936 - 1198 1.3e-62 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 936 - 1195 2e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 612
Acc65I GGTACC 1 cut(s) 1584
AccB1I GGYRCC 2 cut(s) 1584, 2831
AccB7I CCANNNNNTGG 3 cut(s) 515, 2407, 2534
AccII CGCG 1 cut(s) 1976
AciI CCGC 6 cut(s) 740, 1370, 2627, 2727, 2953, 3462
AclI AACGTT 1 cut(s) 3314
AcoI YGGCCR 3 cut(s) 1141, 3236, 3563
AcsI RAATTY 5 cut(s) 20, 158, 495, 1632, 2557
AcuI CTGAAG 5 cut(s) 87, 381, 2328, 2654, 3215
AdeI CACNNNGTG 1 cut(s) 805
AfeI AGCGCT 1 cut(s) 2387
AhdI GACNNNNNGTC 1 cut(s) 1561
Alw21I GWGCWC 1 cut(s) 1696
Alw26I GTCTC 5 cut(s) 914, 2226, 2926, 3367, 3478
Ama87I CYCGRG 1 cut(s) 874
Aor51HI AGCGCT 1 cut(s) 2387
ApeKI GCWGC 9 cut(s) 63, 902, 1045, 1603, 2901, 3143, 3351, 3557, 3611
ApoI RAATTY 5 cut(s) 20, 158, 495, 1632, 2557
ArsI GACNNNNNNTTYG 2 cut(s) 2375, 2407
AseI ATTAAT 1 cut(s) 3125
Asp700I GAANNNNTTC 3 cut(s) 1332, 1632, 2259
Asp718I GGTACC 1 cut(s) 1584
AspLEI GCGC 1 cut(s) 2388
AspS9I GGNCC 9 cut(s) 84, 311, 737, 853, 1232, 2194, 2449, 2548, 3023
AsuC2I CCSGG 2 cut(s) 1474, 3562
AsuHPI GGTGA 7 cut(s) 152, 224, 551, 824, 1724, 1940, 3266
AsuII TTCGAA 1 cut(s) 3091
AsuNHI GCTAGC 1 cut(s) 3614
AvaI CYCGRG 1 cut(s) 874
AvaII GGWCC 4 cut(s) 84, 311, 853, 2449
BalI TGGCCA 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 1738
BanI GGYRCC 2 cut(s) 1584, 2831
BanII GRGCYC 1 cut(s) 3547
BauI CACGAG 2 cut(s) 387, 2265
Bbv12I GWGCWC 1 cut(s) 1696
BbvI GCAGC 9 cut(s) 75, 914, 1032, 1615, 2888, 3155, 3363, 3544, 3623
BceAI ACGGC 2 cut(s) 3251, 3412
BciVI GTATCC 2 cut(s) 1954, 2088
BclI TGATCA 4 cut(s) 1510, 1768, 2425, 2620
BcnI CCSGG 2 cut(s) 1474, 3562
BcoDI GTCTC 5 cut(s) 914, 2226, 2926, 3367, 3478
BfaI CTAG 7 cut(s) 722, 906, 1500, 2804, 3038, 3585, 3615
BfmI CTRYAG 4 cut(s) 692, 1859, 2333, 2940
BfoI RGCGCY 1 cut(s) 2389
BfuI GTATCC 2 cut(s) 1954, 2088
BglII AGATCT 1 cut(s) 2800
BlpI GCTNAGC 1 cut(s) 1905
Bme18I GGWCC 4 cut(s) 84, 311, 853, 2449
BmeRI GACNNNNNGTC 1 cut(s) 1561
BmeT110I CYCGRG 1 cut(s) 874
BmgT120I GGNCC 9 cut(s) 84, 311, 737, 853, 1232, 2194, 2449, 2548, 3023
BmrI ACTGGG 3 cut(s) 2765, 2831, 2919
BmtI GCTAGC 1 cut(s) 3618
BmuI ACTGGG 3 cut(s) 2765, 2831, 2919
BpmI CTGGAG 5 cut(s) 459, 972, 2205, 3315, 3438
Bpu1102I GCTNAGC 1 cut(s) 1905
Bpu14I TTCGAA 1 cut(s) 3091
BpuEI CTTGAG 3 cut(s) 1267, 1327, 1610
BpuMI CCSGG 2 cut(s) 1474, 3562
Bsa29I ATCGAT 1 cut(s) 1663
BsaAI YACGTR 2 cut(s) 1295, 3308
BsaBI GATNNNNATC 2 cut(s) 687, 3102
BsaI GGTCTC 2 cut(s) 3367, 3478
BsaXI ACNNNNNCTCC 4 cut(s) 773, 803, 2854, 2884
Bse3DI GCAATG 3 cut(s) 1130, 1943, 2055
Bse8I GATNNNNATC 2 cut(s) 687, 3102
BseCI ATCGAT 1 cut(s) 1663
BseGI GGATG 9 cut(s) 32, 196, 814, 1831, 2347, 2623, 2983, 3145, 3330
BseJI GATNNNNATC 2 cut(s) 687, 3102
BseMI GCAATG 3 cut(s) 1130, 1943, 2055
BseMII CTCAG 7 cut(s) 50, 538, 810, 1622, 1709, 2576, 3042
BseRI GAGGAG 4 cut(s) 158, 665, 1798, 3437
BseXI GCAGC 9 cut(s) 75, 914, 1032, 1615, 2888, 3155, 3363, 3544, 3623
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 4 cut(s) 782, 1142, 1622, 2246
Bsh1236I CGCG 1 cut(s) 1976
Bsh1285I CGRYCG 1 cut(s) 1667
BshNI GGYRCC 2 cut(s) 1584, 2831
BshVI ATCGAT 1 cut(s) 1663
BsiEI CGRYCG 1 cut(s) 1667
BsiHKAI GWGCWC 1 cut(s) 1696
BsiHKCI CYCGRG 1 cut(s) 874
BsiSI CCGG 3 cut(s) 1473, 2081, 3562
BslFI GGGAC 3 cut(s) 70, 1346, 2854
BsmAI GTCTC 5 cut(s) 914, 2226, 2926, 3367, 3478
BsmFI GGGAC 3 cut(s) 70, 1346, 2854
BsmI GAATGC 2 cut(s) 70, 1906
Bso31I GGTCTC 2 cut(s) 3367, 3478
BsoBI CYCGRG 1 cut(s) 874
Bsp119I TTCGAA 1 cut(s) 3091
Bsp1286I GDGCHC 2 cut(s) 1696, 3547
Bsp1407I TGTACA 2 cut(s) 118, 2516
Bsp1720I GCTNAGC 1 cut(s) 1905
Bsp19I CCATGG 2 cut(s) 3135, 3320
Bsp68I TCGCGA 1 cut(s) 1976
BspACI CCGC 6 cut(s) 740, 1370, 2627, 2727, 2953, 3462
BspCNI CTCAG 7 cut(s) 51, 537, 811, 1621, 1708, 2575, 3043
BspDI ATCGAT 1 cut(s) 1663
BspFNI CGCG 1 cut(s) 1976
BspHI TCATGA 1 cut(s) 2374
BspMAI CTGCAG 3 cut(s) 696, 1863, 2337
BspOI GCTAGC 1 cut(s) 3618
BspQI GCTCTTC 1 cut(s) 1060
BspT104I TTCGAA 1 cut(s) 3091
BspT107I GGYRCC 2 cut(s) 1584, 2831
BspTNI GGTCTC 2 cut(s) 3367, 3478
BsrDI GCAATG 3 cut(s) 1130, 1943, 2055
BsrGI TGTACA 2 cut(s) 118, 2516
BssSI CACGAG 2 cut(s) 387, 2265
BssT1I CCWWGG 4 cut(s) 663, 2292, 3135, 3320
Bst2BI CACGAG 2 cut(s) 387, 2265
Bst4CI ACNGT 8 cut(s) 805, 1024, 1816, 1837, 2845, 2937, 3046, 3506
Bst6I CTCTTC 1 cut(s) 1060
BstAPI GCANNNNNTGC 1 cut(s) 46
BstAUI TGTACA 2 cut(s) 118, 2516
BstBAI YACGTR 2 cut(s) 1295, 3308
BstBI TTCGAA 1 cut(s) 3091
BstC8I GCNNGC 5 cut(s) 68, 1647, 1941, 3119, 3616
BstDSI CCRYGG 2 cut(s) 3135, 3320
BstF5I GGATG 9 cut(s) 32, 196, 814, 1831, 2347, 2623, 2983, 3145, 3330
BstFNI CGCG 1 cut(s) 1976
BstH2I RGCGCY 1 cut(s) 2389
BstHHI GCGC 1 cut(s) 2388
BstMAI GTCTC 5 cut(s) 914, 2226, 2926, 3367, 3478
BstMCI CGRYCG 1 cut(s) 1667
BstMWI GCNNNNNNNGC 7 cut(s) 46, 745, 1070, 1600, 1945, 3022, 3140
BstNSI RCATGY 3 cut(s) 70, 3083, 3442
BstSFI CTRYAG 4 cut(s) 692, 1859, 2333, 2940
BstUI CGCG 1 cut(s) 1976
BstV1I GCAGC 9 cut(s) 75, 914, 1032, 1615, 2888, 3155, 3363, 3544, 3623
BstX2I RGATCY 6 cut(s) 688, 1738, 1989, 2455, 2800, 3226
BstXI CCANNNNNNTGG 1 cut(s) 2459
BstYI RGATCY 6 cut(s) 688, 1738, 1989, 2455, 2800, 3226
Bsu15I ATCGAT 1 cut(s) 1663
BsuI GTATCC 2 cut(s) 1954, 2088
BsuTUI ATCGAT 1 cut(s) 1663
BtgI CCRYGG 2 cut(s) 3135, 3320
BtsCI GGATG 9 cut(s) 32, 196, 814, 1831, 2347, 2623, 2983, 3145, 3330
BtsI GCAGTG 1 cut(s) 3542
BtsIMutI CAGTG 9 cut(s) 778, 801, 1194, 1246, 1278, 2229, 2753, 2907, 3542
BtuMI TCGCGA 1 cut(s) 1976
Cac8I GCNNGC 5 cut(s) 68, 1647, 1941, 3119, 3616
CciI TCATGA 1 cut(s) 2374
CfoI GCGC 1 cut(s) 2388
Cfr13I GGNCC 9 cut(s) 84, 311, 737, 853, 1232, 2194, 2449, 2548, 3023
ClaI ATCGAT 1 cut(s) 1663
CspCI CAANNNNNGTGG 6 cut(s) 189, 224, 1938, 1973, 2976, 3011
DraI TTTAAA 1 cut(s) 2745
DraIII CACNNNGTG 1 cut(s) 805
DriI GACNNNNNGTC 1 cut(s) 1561
EaeI YGGCCR 3 cut(s) 1141, 3236, 3563
Eam1104I CTCTTC 1 cut(s) 1060
Eam1105I GACNNNNNGTC 1 cut(s) 1561
EarI CTCTTC 1 cut(s) 1060
Eco130I CCWWGG 4 cut(s) 663, 2292, 3135, 3320
Eco24I GRGCYC 1 cut(s) 3547
Eco31I GGTCTC 2 cut(s) 3367, 3478
Eco32I GATATC 3 cut(s) 1726, 2248, 2770
Eco47I GGWCC 4 cut(s) 84, 311, 853, 2449
Eco47III AGCGCT 1 cut(s) 2387
Eco57I CTGAAG 5 cut(s) 87, 381, 2328, 2654, 3215
Eco88I CYCGRG 1 cut(s) 874
EcoO109I RGGNCCY 1 cut(s) 84
EcoRI GAATTC 3 cut(s) 20, 1632, 2557
EcoRV GATATC 3 cut(s) 1726, 2248, 2770
EcoT14I CCWWGG 4 cut(s) 663, 2292, 3135, 3320
EcoT22I ATGCAT 3 cut(s) 72, 1107, 1537
EcoT38I GRGCYC 1 cut(s) 3547
ErhI CCWWGG 4 cut(s) 663, 2292, 3135, 3320
FalI AAGNNNNNCTT 2 cut(s) 2888, 2920
FaqI GGGAC 3 cut(s) 70, 1346, 2854
FauI CCCGC 1 cut(s) 2946
FauNDI CATATG 1 cut(s) 2553
FbaI TGATCA 4 cut(s) 1510, 1768, 2425, 2620
FokI GGATG 9 cut(s) 19, 183, 821, 1838, 2354, 2610, 2970, 3152, 3337
FriOI GRGCYC 1 cut(s) 3547
FspBI CTAG 7 cut(s) 722, 906, 1500, 2804, 3038, 3585, 3615
GlaI GCGC 1 cut(s) 2387
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 5 cut(s) 459, 972, 2205, 3315, 3438
HaeII RGCGCY 1 cut(s) 2389
HapII CCGG 3 cut(s) 1473, 2081, 3562
HhaI GCGC 1 cut(s) 2388
Hin6I GCGC 1 cut(s) 2386
HinP1I GCGC 1 cut(s) 2386
HindIII AAGCTT 3 cut(s) 746, 1071, 1907
HpaII CCGG 3 cut(s) 1473, 2081, 3562
HphI GGTGA 7 cut(s) 152, 224, 551, 824, 1724, 1940, 3266
Hpy99I CGWCG 1 cut(s) 3117
HpyCH4III ACNGT 8 cut(s) 805, 1024, 1816, 1837, 2845, 2937, 3046, 3506
HpyCH4IV ACGT 4 cut(s) 1255, 1294, 3307, 3314
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 745, 1070, 1600, 1945, 3022, 3140
HpySE526I ACGT 4 cut(s) 1255, 1294, 3307, 3314
HspAI GCGC 1 cut(s) 2386
KpnI GGTACC 1 cut(s) 1588
Ksp22I TGATCA 4 cut(s) 1510, 1768, 2425, 2620
LguI GCTCTTC 1 cut(s) 1060
Lsp1109I GCAGC 9 cut(s) 75, 914, 1032, 1615, 2888, 3155, 3363, 3544, 3623
MaeI CTAG 7 cut(s) 722, 906, 1500, 2804, 3038, 3585, 3615
MaeII ACGT 4 cut(s) 1255, 1294, 3307, 3314
MaeIII GTNAC 8 cut(s) 254, 812, 1319, 3046, 3200, 3315, 3500, 3635
MfeI CAATTG 4 cut(s) 617, 1224, 1464, 1743
MflI RGATCY 6 cut(s) 688, 1738, 1989, 2455, 2800, 3226
MhlI GDGCHC 2 cut(s) 1696, 3547
MlsI TGGCCA 1 cut(s) 1143
MluNI TGGCCA 1 cut(s) 1143
MlyI GAGTC 2 cut(s) 809, 962
MmeI TCCRAC 2 cut(s) 514, 2388
Mox20I TGGCCA 1 cut(s) 1143
Mph1103I ATGCAT 3 cut(s) 72, 1107, 1537
MroXI GAANNNNTTC 3 cut(s) 1332, 1632, 2259
MscI TGGCCA 1 cut(s) 1143
MslI CAYNNNNRTG 6 cut(s) 201, 426, 915, 1770, 2988, 3649
Msp20I TGGCCA 1 cut(s) 1143
MspA1I CMGCKG 2 cut(s) 1372, 2729
MspI CCGG 3 cut(s) 1473, 2081, 3562
MunI CAATTG 4 cut(s) 617, 1224, 1464, 1743
Mva1269I GAATGC 2 cut(s) 70, 1906
MvnI CGCG 1 cut(s) 1976
MwoI GCNNNNNNNGC 7 cut(s) 46, 745, 1070, 1600, 1945, 3022, 3140
NciI CCSGG 2 cut(s) 1474, 3562
NcoI CCATGG 2 cut(s) 3135, 3320
NdeI CATATG 1 cut(s) 2553
NheI GCTAGC 1 cut(s) 3614
NmeAIII GCCGAG 2 cut(s) 878, 2179
NmuCI GTSAC 5 cut(s) 254, 812, 1319, 3200, 3635
NruI TCGCGA 1 cut(s) 1976
NsiI ATGCAT 3 cut(s) 72, 1107, 1537
NspI RCATGY 3 cut(s) 70, 3083, 3442
NspV TTCGAA 1 cut(s) 3091
PaeI GCATGC 1 cut(s) 70
PaeR7I CTCGAG 1 cut(s) 874
PagI TCATGA 1 cut(s) 2374
PasI CCCWGGG 1 cut(s) 1638
PciSI GCTCTTC 1 cut(s) 1060
PcsI WCGNNNNNNNCGW 3 cut(s) 553, 2263, 3118
PctI GAATGC 2 cut(s) 70, 1906
PdmI GAANNNNTTC 3 cut(s) 1332, 1632, 2259
PfeI GAWTC 9 cut(s) 249, 1210, 1469, 1614, 1660, 1682, 1883, 2690, 3409
PflMI CCANNNNNTGG 3 cut(s) 515, 2407, 2534
PfoI TCCNGGA 2 cut(s) 643, 1472
Ple19I CGATCG 1 cut(s) 1667
PleI GAGTC 2 cut(s) 809, 962
PpsI GAGTC 2 cut(s) 809, 962
Ppu21I YACGTR 2 cut(s) 1295, 3308
PpuMI RGGWCCY 1 cut(s) 84
PshBI ATTAAT 1 cut(s) 3125
PsiI TTATAA 1 cut(s) 612
Psp1406I AACGTT 1 cut(s) 3314
Psp5II RGGWCCY 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 36
PspPI GGNCC 9 cut(s) 84, 311, 737, 853, 1232, 2194, 2449, 2548, 3023
PspPPI RGGWCCY 1 cut(s) 84
PspXI VCTCGAGB 1 cut(s) 874
PsrI GAACNNNNNNTAC 2 cut(s) 3025, 3057
PstI CTGCAG 3 cut(s) 696, 1863, 2337
PsuI RGATCY 6 cut(s) 688, 1738, 1989, 2455, 2800, 3226
PvuI CGATCG 1 cut(s) 1667
RruI TCGCGA 1 cut(s) 1976
RseI CAYNNNNRTG 6 cut(s) 201, 426, 915, 1770, 2988, 3649
SapI GCTCTTC 1 cut(s) 1060
Sau96I GGNCC 9 cut(s) 84, 311, 737, 853, 1232, 2194, 2449, 2548, 3023
SchI GAGTC 2 cut(s) 809, 962
SduI GDGCHC 2 cut(s) 1696, 3547
SfcI CTRYAG 4 cut(s) 692, 1859, 2333, 2940
Sfr274I CTCGAG 1 cut(s) 874
SfuI TTCGAA 1 cut(s) 3091
SinI GGWCC 4 cut(s) 84, 311, 853, 2449
SlaI CTCGAG 1 cut(s) 874
SmiMI CAYNNNNRTG 6 cut(s) 201, 426, 915, 1770, 2988, 3649
SmlI CTYRAG 4 cut(s) 874, 1246, 1342, 1589
SmoI CTYRAG 4 cut(s) 874, 1246, 1342, 1589
SphI GCATGC 1 cut(s) 70
SsiI CCGC 6 cut(s) 740, 1370, 2627, 2727, 2953, 3462
SspI AATATT 3 cut(s) 937, 3161, 3178
SspMI CTAG 7 cut(s) 722, 906, 1500, 2804, 3038, 3585, 3615
StyI CCWWGG 4 cut(s) 663, 2292, 3135, 3320
TaaI ACNGT 8 cut(s) 805, 1024, 1816, 1837, 2845, 2937, 3046, 3506
TaiI ACGT 4 cut(s) 1258, 1297, 3310, 3317
TaqI TCGA 6 cut(s) 172, 875, 1263, 1663, 2250, 3091
TatI WGTACW 4 cut(s) 118, 1866, 2516, 3054
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 9 cut(s) 249, 1210, 1469, 1614, 1660, 1682, 1883, 2690, 3409
TscAI CASTG 9 cut(s) 778, 808, 1194, 1246, 1278, 2229, 2760, 2914, 3542
TseFI GTSAC 5 cut(s) 254, 812, 1319, 3200, 3635
TseI GCWGC 9 cut(s) 63, 902, 1045, 1603, 2901, 3143, 3351, 3557, 3611
Tsp45I GTSAC 5 cut(s) 254, 812, 1319, 3200, 3635
TspGWI ACGGA 2 cut(s) 273, 545
TspRI CASTG 9 cut(s) 778, 808, 1194, 1246, 1278, 2229, 2760, 2914, 3542
Van91I CCANNNNNTGG 3 cut(s) 515, 2407, 2534
VpaK11BI GGWCC 4 cut(s) 84, 311, 853, 2449
VspI ATTAAT 1 cut(s) 3125
XapI RAATTY 5 cut(s) 20, 158, 495, 1632, 2557
XbaI TCTAGA 1 cut(s) 2803
XceI RCATGY 3 cut(s) 70, 3083, 3442
XcmI CCANNNNNNNNNTGG 2 cut(s) 775, 2204
XhoI CTCGAG 1 cut(s) 874
XmnI GAANNNNTTC 3 cut(s) 1332, 1632, 2259
XspI CTAG 7 cut(s) 722, 906, 1500, 2804, 3038, 3585, 3615
Zsp2I ATGCAT 3 cut(s) 72, 1107, 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.