FvH4_3g16100

Translation initiation factor IF-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011012
Physical Location & Seq
Forward (+)
10142024 .. 10149339
7316 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16100.t3

Sequence Viewer

Length: 3087 bp
ATGCTCATATTAGTTGGAAGCATGCAGGGAACAATGGTGTCCTTAGCTTCCCTTGTTAGTTTAGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTGTTAGGAAAGTTTCCTTGTCCAAGACTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGGTTGTCAGTTTGTAAATTTTCAGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTTTCTGTTGATTCTAACTTTAGAGGAAGTGGTAATGATGGTTCTGTTGCCAATGCTGATTGTGTACTTAAGCCGGCGCCAAAGCCAGTGTTGAAGCCCTCTGGGGGGTCCAATGCTGAGCCGCCCCTTTTGAGCCTTAATGCTGCTGAATGGGAGGCCTCGAGAACTGGTGGGGATTCAGATGTAGAAGAGGAAGATAGTAGTAAGGTGATTGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTTGAAGTTCCAAAAGTGGGTGATTCGAGTAAGAATGTCAGTAGACCTGTAAATAGACCTGTGCCATCTAATACAAATACTACTTCAGGAAATGCTAGACCAGTAAACTCGACGGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGTAAAGGAGACACAGTTGCAGCTGTGCAAAAGGTTGTAAAGGAAGTACCTAAGGTTAATAATACAGTTTGGAGAGAAGAACCAAAAACAGGGGGAGGGGTAAAGGTCGAGTCTCCAGCCCGTGCTCCTTTTAGACCTCCTGCTCCACCTTTGAGACCTCAACCAACATTACAAGCAAAGCCTTCCACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTTTGAAGGATCTTGGGGCAGCTCCAAAGTCAGAAGTGATTGATGACACTGGTTCACCCACAAAAACTAAAGAAAGGAAGCCGATTTTGATTGACAAATTTTCTACCAAGAAGACAGGGGTCGATTCTGTGGTTGCTCAAGCAGTTTTAGCTCCTTCAAAACCAGCCAAGGGCTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCCGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTTACTGATGATGAGAGTTCAGAACTCAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCGAGACTCCAGGCTGCCAAAGATGCGGCTCCTGTTAAAGTTGAAATTCTAGAGGTTGAGGAAGATGGCATGTTGATAGATGAATTAGCCTTCAATTTGGCTGTTATGGAATCCGAAATTCTTGGATCTCTGTACTCAAAGGGAATTAAACCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTAAAGATGATTTGTAAAGAGTATGATGTGGAAGTCGTAGATGCTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTTGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTTACTATAATGGGCCATGTGGATCATGGAAAGACAACCCTACTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACACAAGGAATTGGGGCATATAAGGTGCTTGTGCCAATTGATGGCAAGTTGCAGTCATGTGTTTTTCTTGATACTCCTGGACATGAGGCATTTGGAGCGATGAGAGCCCGTGGAGCGAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCAATAGTAATAGCTATAAATAAGATTGACAAGGATGGAGCTAATCCAGAACGAGTGATGCAAGAGCTTTCTTCTATTGGTCTAATGCCGGAGGATTGGGGTGGTGACGTTCCAATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTTTTGGAAACTGTTATGCTAGTTGCAGAGTTGCAAGAGTTAAAGGCCAATCCTGATAGAAGTGCAAAAGGCACAGTCATTGAGGCCGGTCTTGATAAATCTAGAGGACCTTTAGTTACACTTATCGTGCAAAATGGAACCCTTAGAAAGGGGGACATAGTAGTTTGTGGAGAAGCCTTCGGAAAGATACGGGCTTTATTTGATGATGGTGGAAATCGTGTCAATGAAGCTGGACCCTCTATACCTGTTCAGGTTATTGGATTAAATAATGTTCCTGTTGCTGGTGATGAATTTGAGGTTGTGAGCTCCCTTGATATTGCACGTGAAAGAGCAGAGTCACGTGCAGAGTCATTGCGAGATGAACGCATATCGGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCCTTGGCTTCTGCAGTCTCGGCAGGAAAGCTGTCTGGACTTGACTTGCACCAGCTAAATATTATTCTGAAGGTTGATCTTCAGGGATCTATCGAGGCTATCAGACAAGCCCTTCAAGTGCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACAACAGCGATGTGGATCTTGCCGCGGCTAGCAAAGCCATAATTTTGGGATTCAATGTCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAAGGTGTTGAGATTCGGCCATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGTCGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTAAAAGGCTGCGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTACATGTTGGTGTTCTTGATTCTTTAAAACGAGTCAAGGAAGTTGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTACGACGATTTTGAGGAGGGAGACATTCTTGAGGCCTTCAACACAGTCCAAAAGAAGCGGACACTTGAAGAGGCGTCTGCTTCCATGGCAGCTGCGGTGGAAGGAACAGGAGTCGAATACTAG

Protein Analysis

1029

Amino Acids

109.44

Weight (kDa)

6.58

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 504 - 664 3.4e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 505 - 613 1.8e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 679 - 757 2.3e-26 Elongation factor G domain 2
IF-2 PF11987 807 - 901 4.2e-32 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 1643
AccI GTMKAC 1 cut(s) 526
AccII CGCG 1 cut(s) 2576
AccIII TCCGGA 2 cut(s) 1056, 1563
AclWI GGATC 5 cut(s) 870, 1327, 1539, 2470, 2574
AcoI YGGCCR 1 cut(s) 2663
AcsI RAATTY 5 cut(s) 188, 950, 1239, 1311, 2261
AcuI CTGAAG 3 cut(s) 552, 2441, 2465
AcvI CACGTG 2 cut(s) 2294, 2312
AcyI GRCGYC 2 cut(s) 311, 3038
AfaI GTAC 6 cut(s) 300, 604, 681, 1328, 2700, 2874
AfiI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 722, 1022, 1432, 1643, 2119, 2252
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 2875
AhlI ACTAGT 1 cut(s) 137
AjnI CCWGG 4 cut(s) 1033, 1203, 1678, 2617
Alw21I GWGCWC 4 cut(s) 760, 1948, 2279, 2499
Alw26I GTCTC 7 cut(s) 636, 750, 781, 1147, 1192, 2398, 2979
AlwI GGATC 5 cut(s) 870, 1327, 1539, 2470, 2574
AlwNI CAGNNNCTG 1 cut(s) 2624
Ama87I CYCGRG 1 cut(s) 394
Aor13HI TCCGGA 2 cut(s) 1056, 1563
AoxI GGCC 8 cut(s) 390, 1499, 1522, 1785, 2016, 2055, 2663, 2997
ApoI RAATTY 5 cut(s) 188, 950, 1239, 1311, 2261
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 5 cut(s) 342, 1522, 1933, 2078, 2204
AsuC2I CCSGG 1 cut(s) 1077
AsuHPI GGTGA 8 cut(s) 454, 515, 900, 1735, 1931, 2267, 2506, 2555
AsuII TTCGAA 1 cut(s) 2857
AsuNHI GCTAGC 1 cut(s) 2579
AvaI CYCGRG 1 cut(s) 394
AvaII GGWCC 4 cut(s) 342, 1933, 2078, 2204
AxyI CCTNAGG 1 cut(s) 684
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 3 cut(s) 1028, 1711, 2279
BarI GAAGNNNNNNTAC 4 cut(s) 58, 90, 2706, 2738
BbrPI CACGTG 2 cut(s) 2294, 2312
BbsI GAAGAC 3 cut(s) 971, 1500, 2967
Bbv12I GWGCWC 4 cut(s) 760, 1948, 2279, 2499
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 4 cut(s) 1035, 1205, 1680, 2619
BcnI CCSGG 1 cut(s) 1077
BcoDI GTCTC 7 cut(s) 636, 750, 781, 1147, 1192, 2398, 2979
BcuI ACTAGT 1 cut(s) 137
BfmI CTRYAG 4 cut(s) 849, 2388, 2723, 2757
BfoI RGCGCY 1 cut(s) 314
BfrI CTTAAG 1 cut(s) 302
BglI GCCNNNNNGGC 1 cut(s) 1589
BlpI GCTNAGC 2 cut(s) 219, 351
Bme1390I CCNGG 5 cut(s) 1035, 1077, 1205, 1680, 2619
Bme18I GGWCC 4 cut(s) 342, 1933, 2078, 2204
BmeT110I CYCGRG 1 cut(s) 394
BmgT120I GGNCC 5 cut(s) 342, 1522, 1933, 2078, 2204
BmiI GGNNCC 6 cut(s) 312, 343, 1055, 1224, 2110, 2206
BmrFI CCNGG 5 cut(s) 1035, 1077, 1205, 1680, 2619
BmrI ACTGGG 1 cut(s) 1421
BmsI GCATC 3 cut(s) 1207, 1408, 1863
BmtI GCTAGC 1 cut(s) 2583
BmuI ACTGGG 1 cut(s) 1421
BoxI GACNNNNGTC 1 cut(s) 971
BpiI GAAGAC 3 cut(s) 971, 1500, 2967
BpmI CTGGAG 3 cut(s) 732, 1187, 2601
Bpu10I CCTNAGC 2 cut(s) 43, 2346
Bpu1102I GCTNAGC 2 cut(s) 219, 351
Bpu14I TTCGAA 1 cut(s) 2857
BpuEI CTTGAG 3 cut(s) 975, 2487, 3014
BpuMI CCSGG 1 cut(s) 1077
BsaAI YACGTR 2 cut(s) 2294, 2312
BsaBI GATNNNNATC 1 cut(s) 2565
BsaHI GRCGYC 2 cut(s) 311, 3038
BsaI GGTCTC 1 cut(s) 781
BsaJI CCNNGG 7 cut(s) 606, 1020, 1587, 1711, 2379, 2574, 3048
BsaWI WCCGGW 2 cut(s) 1056, 1563
BsaXI ACNNNNNCTCC 6 cut(s) 633, 663, 811, 841, 1707, 1737
Bsc4I CCNNNNNNNGG 9 cut(s) 338, 339, 500, 722, 1022, 1432, 1643, 2119, 2252
Bse118I RCCGGY 2 cut(s) 307, 2057
Bse1I ACTGG 6 cut(s) 320, 406, 584, 907, 1427, 1557
Bse21I CCTNAGG 1 cut(s) 684
Bse3DI GCAATG 3 cut(s) 235, 2321, 2715
Bse8I GATNNNNATC 1 cut(s) 2565
BseAI TCCGGA 2 cut(s) 1056, 1563
BseBI CCWGG 4 cut(s) 1035, 1205, 1680, 2619
BseDI CCNNGG 7 cut(s) 606, 1020, 1587, 1711, 2379, 2574, 3048
BseGI GGATG 3 cut(s) 89, 1560, 1855
BseJI GATNNNNATC 1 cut(s) 2565
BseLI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 722, 1022, 1432, 1643, 2119, 2252
BseMI GCAATG 3 cut(s) 235, 2321, 2715
BseMII CTCAG 3 cut(s) 210, 342, 1355
BseNI ACTGG 6 cut(s) 320, 406, 584, 907, 1427, 1557
BseRI GAGGAG 2 cut(s) 816, 2993
BsgI GTGCAG 1 cut(s) 2334
Bsh1236I CGCG 1 cut(s) 2576
BshFI GGCC 8 cut(s) 392, 1501, 1524, 1787, 2018, 2057, 2665, 2999
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 4 cut(s) 760, 1948, 2279, 2499
BsiHKCI CYCGRG 1 cut(s) 394
BsiSI CCGG 6 cut(s) 308, 1057, 1077, 1564, 1904, 2058
BslFI GGGAC 1 cut(s) 2138
BslI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 722, 1022, 1432, 1643, 2119, 2252
BsmAI GTCTC 7 cut(s) 636, 750, 781, 1147, 1192, 2398, 2979
BsmFI GGGAC 1 cut(s) 2138
BsmI GAATGC 1 cut(s) 2844
BsnI GGCC 8 cut(s) 392, 1501, 1524, 1787, 2018, 2057, 2665, 2999
Bso31I GGTCTC 1 cut(s) 781
BsoBI CYCGRG 1 cut(s) 394
Bsp119I TTCGAA 1 cut(s) 2857
Bsp1286I GDGCHC 6 cut(s) 760, 1028, 1711, 1948, 2279, 2499
Bsp13I TCCGGA 2 cut(s) 1056, 1563
Bsp143I GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
Bsp1720I GCTNAGC 2 cut(s) 219, 351
Bsp19I CCATGG 1 cut(s) 3048
BspANI GGCC 8 cut(s) 392, 1501, 1524, 1787, 2018, 2057, 2665, 2999
BspCNI CTCAG 3 cut(s) 211, 343, 1356
BspEI TCCGGA 2 cut(s) 1056, 1563
BspFNI CGCG 1 cut(s) 2576
BspLI GGNNCC 6 cut(s) 312, 343, 1055, 1224, 2110, 2206
BspMAI CTGCAG 2 cut(s) 2392, 2761
BspOI GCTAGC 1 cut(s) 2583
BspPI GGATC 5 cut(s) 870, 1327, 1539, 2470, 2574
BspQI GCTCTTC 1 cut(s) 1562
BspT104I TTCGAA 1 cut(s) 2857
BspT107I GGYRCC 1 cut(s) 310
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 781
BsrDI GCAATG 3 cut(s) 235, 2321, 2715
BsrFI RCCGGY 2 cut(s) 307, 2057
BsrI ACTGG 6 cut(s) 320, 406, 584, 907, 1427, 1557
BssAI RCCGGY 2 cut(s) 307, 2057
BssECI CCNNGG 7 cut(s) 606, 1020, 1587, 1711, 2379, 2574, 3048
BssMI GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
BssNI GRCGYC 2 cut(s) 311, 3038
BssT1I CCWWGG 4 cut(s) 606, 1020, 2379, 3048
Bst2UI CCWGG 4 cut(s) 1035, 1205, 1680, 2619
Bst4CI ACNGT 5 cut(s) 649, 700, 1984, 2047, 3010
Bst6I CTCTTC 4 cut(s) 417, 617, 1562, 3027
BstACI GRCGYC 2 cut(s) 311, 3038
BstAFI CTTAAG 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 2294, 2312
BstBI TTCGAA 1 cut(s) 2857
BstC8I GCNNGC 7 cut(s) 23, 81, 107, 309, 1183, 2353, 2581
BstDEI CTNAG 7 cut(s) 43, 219, 351, 684, 1364, 2114, 2346
BstDSI CCRYGG 3 cut(s) 1711, 2574, 3048
BstENI CCTNNNNNAGG 1 cut(s) 2117
BstF5I GGATG 3 cut(s) 89, 1560, 1855
BstFNI CGCG 1 cut(s) 2576
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
BstMAI GTCTC 7 cut(s) 636, 750, 781, 1147, 1192, 2398, 2979
BstMBI GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
BstNI CCWGG 4 cut(s) 1035, 1205, 1680, 2619
BstNSI RCATGY 3 cut(s) 25, 1267, 2879
BstPAI GACNNNNGTC 1 cut(s) 971
BstSCI CCNGG 5 cut(s) 1033, 1075, 1203, 1678, 2617
BstSFI CTRYAG 4 cut(s) 849, 2388, 2723, 2757
BstUI CGCG 1 cut(s) 2576
BstV2I GAAGAC 3 cut(s) 971, 1500, 2967
BstX2I RGATCY 4 cut(s) 862, 1319, 2462, 2566
BstXI CCANNNNNNTGG 1 cut(s) 2596
BstYI RGATCY 4 cut(s) 862, 1319, 2462, 2566
Bsu36I CCTNAGG 1 cut(s) 684
BsuRI GGCC 8 cut(s) 392, 1501, 1524, 1787, 2018, 2057, 2665, 2999
BtgI CCRYGG 3 cut(s) 1711, 2574, 3048
BtgZI GCGATG 4 cut(s) 1715, 1774, 2370, 2574
BtsCI GGATG 3 cut(s) 89, 1560, 1855
BtsI GCAGTG 2 cut(s) 2788, 2794
BtsIMutI CAGTG 4 cut(s) 327, 900, 2788, 2794
Cac8I GCNNGC 7 cut(s) 23, 81, 107, 309, 1183, 2353, 2581
CaiI CAGNNNCTG 1 cut(s) 2624
CfoI GCGC 1 cut(s) 313
Cfr10I RCCGGY 2 cut(s) 307, 2057
Cfr13I GGNCC 5 cut(s) 342, 1522, 1933, 2078, 2204
Cfr42I CCGCGG 1 cut(s) 2577
CseI GACGC 1 cut(s) 3027
Csp6I GTAC 6 cut(s) 299, 603, 680, 1327, 2699, 2873
CviQI GTAC 6 cut(s) 299, 603, 680, 1327, 2699, 2873
DdeI CTNAG 7 cut(s) 43, 219, 351, 684, 1364, 2114, 2346
DinI GGCGCC 1 cut(s) 312
DpnI GATC 6 cut(s) 864, 1321, 1533, 2454, 2464, 2568
DpnII GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
DraI TTTAAA 1 cut(s) 2898
EaeI YGGCCR 1 cut(s) 2663
Eam1104I CTCTTC 4 cut(s) 417, 617, 1562, 3027
EarI CTCTTC 4 cut(s) 417, 617, 1562, 3027
EciI GGCGGA 3 cut(s) 1020, 1108, 1757
Ecl136II GAGCTC 1 cut(s) 2277
Eco130I CCWWGG 4 cut(s) 606, 1020, 2379, 3048
Eco147I AGGCCT 3 cut(s) 392, 1501, 2999
Eco24I GRGCYC 3 cut(s) 1028, 1711, 2279
Eco31I GGTCTC 1 cut(s) 781
Eco47I GGWCC 4 cut(s) 342, 1933, 2078, 2204
Eco53kI GAGCTC 1 cut(s) 2277
Eco57I CTGAAG 3 cut(s) 552, 2441, 2465
Eco72I CACGTG 2 cut(s) 2294, 2312
Eco81I CCTNAGG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 394
EcoICRI GAGCTC 1 cut(s) 2277
EcoNI CCTNNNNNAGG 1 cut(s) 2117
EcoO109I RGGNCCY 1 cut(s) 2078
EcoRII CCWGG 4 cut(s) 1033, 1203, 1678, 2617
EcoT14I CCWWGG 4 cut(s) 606, 1020, 2379, 3048
EcoT38I GRGCYC 3 cut(s) 1028, 1711, 2279
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
ErhI CCWWGG 4 cut(s) 606, 1020, 2379, 3048
FalI AAGNNNNNCTT 2 cut(s) 1449, 1481
FaqI GGGAC 1 cut(s) 2138
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 526
FokI GGATG 3 cut(s) 76, 1547, 1862
FriOI GRGCYC 3 cut(s) 1028, 1711, 2279
GlaI GCGC 1 cut(s) 312
GsuI CTGGAG 3 cut(s) 732, 1187, 2601
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 8 cut(s) 392, 1501, 1524, 1787, 2018, 2057, 2665, 2999
HapII CCGG 6 cut(s) 308, 1057, 1077, 1564, 1904, 2058
HgaI GACGC 1 cut(s) 3027
HhaI GCGC 1 cut(s) 313
Hin1I GRCGYC 2 cut(s) 311, 3038
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HindIII AAGCTT 1 cut(s) 482
HpaII CCGG 6 cut(s) 308, 1057, 1077, 1564, 1904, 2058
HphI GGTGA 8 cut(s) 454, 515, 900, 1735, 1931, 2267, 2506, 2555
Hpy166II GTNNAC 5 cut(s) 299, 527, 589, 908, 2551
Hpy8I GTNNAC 5 cut(s) 299, 527, 589, 908, 2551
Hpy99I CGWCG 2 cut(s) 598, 2972
HpyCH4III ACNGT 5 cut(s) 649, 700, 1984, 2047, 3010
HpyCH4IV ACGT 3 cut(s) 1923, 2293, 2311
HpyF3I CTNAG 7 cut(s) 43, 219, 351, 684, 1364, 2114, 2346
HpySE526I ACGT 3 cut(s) 1923, 2293, 2311
Hsp92I GRCGYC 2 cut(s) 311, 3038
HspAI GCGC 1 cut(s) 311
KasI GGCGCC 1 cut(s) 310
Kpn2I TCCGGA 2 cut(s) 1056, 1563
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
KspI CCGCGG 1 cut(s) 2577
Kzo9I GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
LguI GCTCTTC 1 cut(s) 1562
LweI GCATC 3 cut(s) 1207, 1408, 1863
MaeII ACGT 3 cut(s) 1923, 2293, 2311
MalI GATC 6 cut(s) 864, 1321, 1533, 2454, 2464, 2568
MboI GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
MfeI CAATTG 2 cut(s) 1638, 2748
MflI RGATCY 4 cut(s) 862, 1319, 2462, 2566
MhlI GDGCHC 6 cut(s) 760, 1028, 1711, 1948, 2279, 2499
Mly113I GGCGCC 1 cut(s) 311
MlyI GAGTC 8 cut(s) 458, 752, 1194, 2315, 2327, 2712, 2913, 3084
MroI TCCGGA 2 cut(s) 1056, 1563
MroNI GCCGGC 1 cut(s) 307
MslI CAYNNNNRTG 3 cut(s) 228, 1745, 2880
MspA1I CMGCKG 3 cut(s) 656, 2576, 3056
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 6 cut(s) 308, 1057, 1077, 1564, 1904, 2058
MspR9I CCNGG 5 cut(s) 1035, 1077, 1205, 1680, 2619
MunI CAATTG 2 cut(s) 1638, 2748
Mva1269I GAATGC 1 cut(s) 2844
MvaI CCWGG 4 cut(s) 1035, 1205, 1680, 2619
MvnI CGCG 1 cut(s) 2576
NaeI GCCGGC 1 cut(s) 309
NarI GGCGCC 1 cut(s) 311
NciI CCSGG 1 cut(s) 1077
NcoI CCATGG 1 cut(s) 3048
NdeII GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
NgoMIV GCCGGC 1 cut(s) 307
NheI GCTAGC 1 cut(s) 2579
NlaIV GGNNCC 6 cut(s) 312, 343, 1055, 1224, 2110, 2206
NmeAIII GCCGAG 1 cut(s) 2375
NmuCI GTSAC 6 cut(s) 196, 450, 1723, 1919, 2307, 2512
NspI RCATGY 3 cut(s) 25, 1267, 2879
NspV TTCGAA 1 cut(s) 2857
PaeI GCATGC 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 394
PceI AGGCCT 3 cut(s) 392, 1501, 2999
PciI ACATGT 1 cut(s) 2875
PciSI GCTCTTC 1 cut(s) 1562
PctI GAATGC 1 cut(s) 2844
PdiI GCCGGC 1 cut(s) 309
PflMI CCANNNNNTGG 1 cut(s) 1643
PfoI TCCNGGA 1 cut(s) 1678
PleI GAGTC 8 cut(s) 457, 751, 1194, 2314, 2326, 2712, 2912, 3083
PluTI GGCGCC 1 cut(s) 314
PmaCI CACGTG 2 cut(s) 2294, 2312
PmlI CACGTG 2 cut(s) 2294, 2312
PpsI GAGTC 8 cut(s) 457, 751, 1194, 2314, 2326, 2712, 2912, 3083
Ppu21I YACGTR 2 cut(s) 2294, 2312
PpuMI RGGWCCY 1 cut(s) 2078
PscI ACATGT 1 cut(s) 2875
PshAI GACNNNNGTC 1 cut(s) 971
Psp124BI GAGCTC 1 cut(s) 2279
Psp5II RGGWCCY 1 cut(s) 2078
Psp6I CCWGG 4 cut(s) 1033, 1203, 1678, 2617
PspCI CACGTG 2 cut(s) 2294, 2312
PspGI CCWGG 4 cut(s) 1033, 1203, 1678, 2617
PspN4I GGNNCC 6 cut(s) 312, 343, 1055, 1224, 2110, 2206
PspPI GGNCC 5 cut(s) 342, 1522, 1933, 2078, 2204
PspPPI RGGWCCY 1 cut(s) 2078
PsrI GAACNNNNNNTAC 2 cut(s) 259, 291
PstI CTGCAG 2 cut(s) 2392, 2761
PstNI CAGNNNCTG 1 cut(s) 2624
PsuI RGATCY 4 cut(s) 862, 1319, 2462, 2566
PvuII CAGCTG 2 cut(s) 656, 3056
RsaI GTAC 6 cut(s) 300, 604, 681, 1328, 2700, 2874
RsaNI GTAC 6 cut(s) 299, 603, 680, 1327, 2699, 2873
RseI CAYNNNNRTG 3 cut(s) 228, 1745, 2880
SacI GAGCTC 1 cut(s) 2279
SacII CCGCGG 1 cut(s) 2577
SapI GCTCTTC 1 cut(s) 1562
Sau3AI GATC 6 cut(s) 862, 1319, 1531, 2452, 2462, 2566
Sau96I GGNCC 5 cut(s) 342, 1522, 1933, 2078, 2204
SchI GAGTC 8 cut(s) 458, 752, 1194, 2315, 2327, 2712, 2913, 3084
ScrFI CCNGG 5 cut(s) 1035, 1077, 1205, 1680, 2619
SduI GDGCHC 6 cut(s) 760, 1028, 1711, 1948, 2279, 2499
SfaNI GCATC 3 cut(s) 1207, 1408, 1863
SfcI CTRYAG 4 cut(s) 849, 2388, 2723, 2757
SfoI GGCGCC 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 394
Sfr303I CCGCGG 1 cut(s) 2577
SfuI TTCGAA 1 cut(s) 2857
SgrBI CCGCGG 1 cut(s) 2577
SinI GGWCC 4 cut(s) 342, 1933, 2078, 2204
SlaI CTCGAG 1 cut(s) 394
SmiMI CAYNNNNRTG 3 cut(s) 228, 1745, 2880
SmlI CTYRAG 5 cut(s) 302, 394, 990, 2502, 2993
SmoI CTYRAG 5 cut(s) 302, 394, 990, 2502, 2993
SpeI ACTAGT 1 cut(s) 137
SphI GCATGC 1 cut(s) 25
SseBI AGGCCT 3 cut(s) 392, 1501, 2999
SspDI GGCGCC 1 cut(s) 310
SspI AATATT 1 cut(s) 2437
SstI GAGCTC 1 cut(s) 2279
StuI AGGCCT 3 cut(s) 392, 1501, 2999
StyD4I CCNGG 5 cut(s) 1033, 1075, 1203, 1678, 2617
StyI CCWWGG 4 cut(s) 606, 1020, 2379, 3048
TaaI ACNGT 5 cut(s) 649, 700, 1984, 2047, 3010
TaiI ACGT 3 cut(s) 1926, 2296, 2314
TaqI TCGA 8 cut(s) 395, 509, 593, 741, 975, 2469, 2857, 3078
TatI WGTACW 3 cut(s) 298, 1326, 2872
TauI GCSGC 6 cut(s) 358, 1196, 1223, 2576, 2579, 2844
TscAI CASTG 4 cut(s) 327, 907, 2788, 2794
TseFI GTSAC 6 cut(s) 196, 450, 1723, 1919, 2307, 2512
Tsp45I GTSAC 6 cut(s) 196, 450, 1723, 1919, 2307, 2512
TspDTI ATGAA 7 cut(s) 78, 1290, 1485, 2211, 2274, 2346, 2832
TspRI CASTG 4 cut(s) 327, 907, 2788, 2794
Van91I CCANNNNNTGG 1 cut(s) 1643
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 4 cut(s) 342, 1933, 2078, 2204
XagI CCTNNNNNAGG 1 cut(s) 2117
XapI RAATTY 5 cut(s) 188, 950, 1239, 1311, 2261
XbaI TCTAGA 2 cut(s) 1243, 2072
XceI RCATGY 3 cut(s) 25, 1267, 2879
XcmI CCANNNNNNNNNTGG 1 cut(s) 1532
XhoI CTCGAG 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.