RchiOBHm_Chr5g0027081

Translation initiation factor IF-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011012
Physical Location & Seq
Forward (+)
21031415 .. 21039214
7800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30663

Sequence Viewer

Length: 3090 bp
ATGTTCATATTAGTTGGAAGCATGCAGGGAACAATGGTTTCTTTGGCTTCCCTTGTTAGTTTGGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTGTTAGGAAGGTTTCGTTGTCTAAGAGTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGATTGTCAGTTTGTAAATTTTCGGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTATCCCTTGATTCTAACTATAGAGGAAGTAGTAATGATGCTTCTGTTGCCAATGCTGATTTTGTACTTAAGCCGTCCCCGAAGCCAGTATTGAAGCCCTCTGAGGGGTCCAATGCTGAACCGCCCCTTTTAAGCCTTAATGCTGCTGATTGGGACGCCTCAAAAACTAGTGGGGATTCGGATGTGGAAGAGGAAGATAAGAGTAATGTGATTGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTAGAAGCTCCAAAAGTGGATGATTTGAGTACTAAGAAAGCCAGTAAGCCTGTAAATAGACCTGCACCTTCTAATGCAACTACTACTTCAGGAAATGCTAGACCAGTAAACTCGACAGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGAAAAGGAGACACAGTTGCAGCTGTGCAAAAGGTTGTAAAGGAATTACCTAAGGTTAATAATACAGTTCGGAGAGAAGAACCAAAAACAGGGGGAGGGGTGAAGGTAGAGTCTACACCCGGTCCTCCTTTAAGACCTCCTCCTCCACCTTTGAGACCCCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTCTGAAGGATCTTGGGGCAGCTCCAAAGTCGGCCGTGATTGATGACACTGGTTCACCCCCAAAAACTAAAGAAAGGAAGCCAATTTTGATTGACAAATTTTCTACCAAGAAGCCAGGGGTCGATTCTGTGGTTTCTCAAGCAGTTTTAGCTCCTACAAAACCAGCCAAGGGCTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCTGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTCACTGATGATGAGAGTTCGGAACTCAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCAAGACTCCAGGCTGCCAAAGATGCAGCTCCTGTTAAAGTAGATATTCTAGAGGTTGATGACGATGGCATGTTAATAGATGAGTTGGCCTTCAATTTGGCTATTAATGAATCCGAAATTCTCGGATCTCTGTACTCCAGGGGAATTAAGCCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTGAAGATGATTTGTAAAGAGTATGATGTGGAAGTCATAGATGTTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTCGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTTACTATAATGGGTCATGTGGATCATGGCAAGACAACTCTATTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACGCAAGGAATTGGGGCATACAAGGTGCTTGTGCCAGTTGATGGCAAGTTGCAATCATGTATTTTCCTTGATACTCCTGGACATGAGGCATTTGGAGCAATGAGAGCTCGTGGAGCAAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCGATAGTAATAGCTATAAATAAGATAGACAAGGATGGAGCTAATCCAGAACGAGTCATGCAAGAGCTTTCTTCTATAGGTCTAATGCCGGAGGATTGGGGTGGTGACGTCCCCATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTTTTGGAAACTGTAATGCTAGTTGCAGAGTTGCAAGAGTTAAAGGCCAATCCTGATAGAAGTGCAAAAGGCACGGTTATTGAGGCCGGTCTTGATAAATCCAGAGGACCTTTAGTTACACTTATCGTGCAAAATGGAACCCTTAGAAAGGGGGACATAGTAGTTTGTGGAGAAGCCTTTGGAAAGGTACGGGCTTTATTTGATGATGGTGGAAATCGTGTCAGTGAAGCTGGACCCTCTATACCAGTTCAGGTTATTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAGGTCGTGAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTCACGTGCAGAGTCATTGCGAAATGAACGCATATCAGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCTTTAGCTTCTGCAGTCTCGGCAGGAAAGCTGGCTGGACTTGACTTGCACCAGCTAAATGTTATTCTGAAGGTTGATCTTCAGGGATCTATTGAGGCTGTCAGACAAGCCCTGCAGGTGCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACACCAGCGATGTTGATCTTGCTGCGGCCAGCAAAGCCATAATTTTGGGATTCAATATCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAGGGTGTGGAGATTCGGCTATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGACGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTTAAAGGCTGTGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTTCATGTTGGTGTTCTTGATTCTTTAAAACGGGTTAAGGAAGTCGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTATGACGATTTTGAGGAGGGAGACATACTCGAGGCCTTCAACACAGTCCAAAAGAAGCGGACACTTGAAGAGGCGTCTGCTTCCATGGCAGCTGCAGTGGAAGGAACAGGAGTCGAATACTAG

Protein Analysis

1029

Amino Acids

109.58

Weight (kDa)

6.8

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 505 - 665 3.7e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 506 - 614 1.8e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 680 - 758 3e-26 Elongation factor G domain 2
IF-2 PF11987 808 - 902 1.7e-31 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2485
AatII GACGTC 1 cut(s) 1929
Acc36I ACCTGC 2 cut(s) 553, 2485
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccI GTMKAC 1 cut(s) 749
AccIII TCCGGA 2 cut(s) 1059, 1566
AciI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 3025
AclWI GGATC 4 cut(s) 873, 1330, 1542, 2473
AcoI YGGCCR 2 cut(s) 888, 2580
AcsI RAATTY 4 cut(s) 188, 953, 1314, 2264
AcuI CTGAAG 4 cut(s) 555, 881, 2444, 2468
AcvI CACGTG 2 cut(s) 2297, 2315
AcyI GRCGYC 3 cut(s) 390, 1926, 3041
AfaI GTAC 6 cut(s) 300, 514, 607, 1331, 2163, 2703
AfiI CCNNNNNNNGG 8 cut(s) 338, 339, 725, 1025, 1435, 1646, 2122, 2255
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 2797
AgsI TTSAA 9 cut(s) 328, 625, 1048, 1291, 1361, 2524, 2608, 3007, 3035
AhlI ACTAGT 1 cut(s) 401
AjiI CACGTC 1 cut(s) 2798
AjnI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
AloI GAACNNNNNNTCC 2 cut(s) 2859, 2891
Alw21I GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
Alw26I GTCTC 5 cut(s) 639, 784, 1150, 2401, 2982
AlwI GGATC 4 cut(s) 873, 1330, 1542, 2473
AlwNI CAGNNNCTG 2 cut(s) 1229, 2627
Ama87I CYCGRG 1 cut(s) 2996
Aor13HI TCCGGA 2 cut(s) 1059, 1566
AoxI GGCC 8 cut(s) 888, 1284, 1502, 1788, 2019, 2058, 2580, 3000
ApoI RAATTY 4 cut(s) 188, 953, 1314, 2264
AseI ATTAAT 1 cut(s) 1302
AspS9I GGNCC 5 cut(s) 342, 758, 1936, 2081, 2207
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 8 cut(s) 748, 903, 1393, 1738, 1934, 2270, 2509, 2558
AsuII TTCGAA 1 cut(s) 2860
AvaI CYCGRG 1 cut(s) 2996
AvaII GGWCC 5 cut(s) 342, 758, 1936, 2081, 2207
AxyI CCTNAGG 1 cut(s) 687
BanII GRGCYC 4 cut(s) 1031, 1116, 1714, 2282
BarI GAAGNNNNNNTAC 6 cut(s) 58, 90, 259, 291, 2709, 2741
BauI CACGAG 1 cut(s) 1713
BbrPI CACGTG 2 cut(s) 2297, 2315
BbsI GAAGAC 2 cut(s) 1503, 2970
Bbv12I GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
BceAI ACGGC 2 cut(s) 292, 875
BciT130I CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 5 cut(s) 639, 784, 1150, 2401, 2982
BcuI ACTAGT 1 cut(s) 401
BfmI CTRYAG 9 cut(s) 253, 822, 852, 1893, 2391, 2492, 2726, 2760, 3060
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 2 cut(s) 553, 2485
BfuI GTATCC 1 cut(s) 247
BglI GCCNNNNNGGC 1 cut(s) 1592
BlpI GCTNAGC 1 cut(s) 219
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2622
Bme18I GGWCC 5 cut(s) 342, 758, 1936, 2081, 2207
BmeT110I CYCGRG 1 cut(s) 2996
BmgBI CACGTC 1 cut(s) 2798
BmgT120I GGNCC 5 cut(s) 342, 758, 1936, 2081, 2207
BmiI GGNNCC 4 cut(s) 343, 1058, 2113, 2209
BmrFI CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2622
BmrI ACTGGG 1 cut(s) 1424
BmsI GCATC 2 cut(s) 262, 1210
BmuI ACTGGG 1 cut(s) 1424
BpiI GAAGAC 2 cut(s) 1503, 2970
BplI GAGNNNNNCTC 2 cut(s) 2979, 3011
BpmI CTGGAG 4 cut(s) 1101, 1190, 1318, 2604
Bpu10I CCTNAGC 1 cut(s) 2349
Bpu1102I GCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 2860
BpuEI CTTGAG 2 cut(s) 978, 2490
BpuMI CCSGG 1 cut(s) 756
BsaAI YACGTR 2 cut(s) 2297, 2315
BsaBI GATNNNNATC 1 cut(s) 2568
BsaHI GRCGYC 3 cut(s) 390, 1926, 3041
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 7 cut(s) 609, 971, 1023, 1335, 1590, 1932, 3051
BsaWI WCCGGW 2 cut(s) 1059, 1566
BsaXI ACNNNNNCTCC 6 cut(s) 636, 666, 814, 844, 1710, 1740
Bsc4I CCNNNNNNNGG 8 cut(s) 338, 339, 725, 1025, 1435, 1646, 2122, 2255
Bse118I RCCGGY 1 cut(s) 2060
Bse1I ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 2219
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 4 cut(s) 235, 1710, 2324, 2718
Bse8I GATNNNNATC 1 cut(s) 2568
BseAI TCCGGA 2 cut(s) 1059, 1566
BseBI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BseDI CCNNGG 7 cut(s) 609, 971, 1023, 1335, 1590, 1932, 3051
BseGI GGATG 5 cut(s) 89, 421, 508, 1563, 1858
BseJI GATNNNNATC 1 cut(s) 2568
BseLI CCNNNNNNNGG 8 cut(s) 338, 339, 725, 1025, 1435, 1646, 2122, 2255
BseMI GCAATG 4 cut(s) 235, 1710, 2324, 2718
BseMII CTCAG 3 cut(s) 210, 327, 1358
BseNI ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 2219
BseRI GAGGAG 4 cut(s) 765, 768, 819, 2996
BseX3I CGGCCG 1 cut(s) 888
BsgI GTGCAG 2 cut(s) 531, 2337
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 8 cut(s) 890, 1286, 1504, 1790, 2021, 2060, 2582, 3002
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
BsiHKCI CYCGRG 1 cut(s) 2996
BsiSI CCGG 5 cut(s) 756, 1060, 1567, 1907, 2061
BslFI GGGAC 4 cut(s) 295, 401, 1913, 2141
BslI CCNNNNNNNGG 8 cut(s) 338, 339, 725, 1025, 1435, 1646, 2122, 2255
BsmAI GTCTC 5 cut(s) 639, 784, 1150, 2401, 2982
BsmFI GGGAC 4 cut(s) 295, 401, 1913, 2141
BsmI GAATGC 1 cut(s) 2847
BsnI GGCC 8 cut(s) 890, 1286, 1504, 1790, 2021, 2060, 2582, 3002
Bso31I GGTCTC 1 cut(s) 784
BsoBI CYCGRG 1 cut(s) 2996
Bsp119I TTCGAA 1 cut(s) 2860
Bsp1286I GDGCHC 6 cut(s) 1031, 1116, 1714, 1951, 2282, 2502
Bsp13I TCCGGA 2 cut(s) 1059, 1566
Bsp143I GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 2 cut(s) 1932, 3051
BspACI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 3025
BspANI GGCC 8 cut(s) 890, 1286, 1504, 1790, 2021, 2060, 2582, 3002
BspCNI CTCAG 3 cut(s) 211, 328, 1359
BspEI TCCGGA 2 cut(s) 1059, 1566
BspLI GGNNCC 4 cut(s) 343, 1058, 2113, 2209
BspMAI CTGCAG 4 cut(s) 2395, 2496, 2764, 3064
BspMI ACCTGC 2 cut(s) 553, 2485
BspPI GGATC 4 cut(s) 873, 1330, 1542, 2473
BspQI GCTCTTC 1 cut(s) 1565
BspT104I TTCGAA 1 cut(s) 2860
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 784
BsrDI GCAATG 4 cut(s) 235, 1710, 2324, 2718
BsrFI RCCGGY 1 cut(s) 2060
BsrI ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 2219
BssAI RCCGGY 1 cut(s) 2060
BssECI CCNNGG 7 cut(s) 609, 971, 1023, 1335, 1590, 1932, 3051
BssMI GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
BssNI GRCGYC 3 cut(s) 390, 1926, 3041
BssSI CACGAG 1 cut(s) 1713
BssT1I CCWWGG 4 cut(s) 609, 1023, 1932, 3051
Bst2BI CACGAG 1 cut(s) 1713
Bst2UI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
Bst4CI ACNGT 5 cut(s) 652, 703, 1987, 2050, 3013
Bst6I CTCTTC 4 cut(s) 417, 620, 1565, 3030
BstACI GRCGYC 3 cut(s) 390, 1926, 3041
BstAFI CTTAAG 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 2297, 2315
BstBI TTCGAA 1 cut(s) 2860
BstC8I GCNNGC 7 cut(s) 23, 81, 107, 1186, 2356, 2412, 2584
BstDEI CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
BstDSI CCRYGG 2 cut(s) 1932, 3051
BstENI CCTNNNNNAGG 1 cut(s) 2120
BstF5I GGATG 5 cut(s) 89, 421, 508, 1563, 1858
BstKTI GATC 6 cut(s) 868, 1325, 1537, 2458, 2468, 2572
BstMAI GTCTC 5 cut(s) 639, 784, 1150, 2401, 2982
BstMBI GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
BstMCI CGRYCG 1 cut(s) 891
BstNI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BstNSI RCATGY 2 cut(s) 25, 1270
BstSCI CCNGG 8 cut(s) 754, 970, 1036, 1078, 1206, 1334, 1681, 2620
BstSFI CTRYAG 9 cut(s) 253, 822, 852, 1893, 2391, 2492, 2726, 2760, 3060
BstV2I GAAGAC 2 cut(s) 1503, 2970
BstX2I RGATCY 3 cut(s) 865, 1322, 2465
BstXI CCANNNNNNTGG 1 cut(s) 2599
BstYI RGATCY 3 cut(s) 865, 1322, 2465
BstZI CGGCCG 1 cut(s) 888
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 8 cut(s) 890, 1286, 1504, 1790, 2021, 2060, 2582, 3002
BtgI CCRYGG 2 cut(s) 1932, 3051
BtgZI GCGATG 3 cut(s) 1777, 2373, 2577
BtrI CACGTC 1 cut(s) 2798
BtsCI GGATG 5 cut(s) 89, 421, 508, 1563, 1858
BtsI GCAGTG 3 cut(s) 2791, 2797, 3069
BtsIMutI CAGTG 6 cut(s) 903, 1116, 2203, 2791, 2797, 3069
BveI ACCTGC 2 cut(s) 553, 2485
Cac8I GCNNGC 7 cut(s) 23, 81, 107, 1186, 2356, 2412, 2584
CaiI CAGNNNCTG 2 cut(s) 1229, 2627
Cfr10I RCCGGY 1 cut(s) 2060
Cfr13I GGNCC 5 cut(s) 342, 758, 1936, 2081, 2207
CseI GACGC 2 cut(s) 398, 3030
Csp6I GTAC 6 cut(s) 299, 513, 606, 1330, 2162, 2702
CviQI GTAC 6 cut(s) 299, 513, 606, 1330, 2162, 2702
DdeI CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
DpnI GATC 6 cut(s) 867, 1324, 1536, 2457, 2467, 2571
DpnII GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
DraI TTTAAA 1 cut(s) 2901
EaeI YGGCCR 2 cut(s) 888, 2580
EagI CGGCCG 1 cut(s) 888
Eam1104I CTCTTC 4 cut(s) 417, 620, 1565, 3030
EarI CTCTTC 4 cut(s) 417, 620, 1565, 3030
EciI GGCGGA 3 cut(s) 1023, 1111, 1760
Ecl136II GAGCTC 3 cut(s) 1114, 1712, 2280
EclXI CGGCCG 1 cut(s) 888
Eco130I CCWWGG 4 cut(s) 609, 1023, 1932, 3051
Eco147I AGGCCT 2 cut(s) 1504, 3002
Eco24I GRGCYC 4 cut(s) 1031, 1116, 1714, 2282
Eco31I GGTCTC 1 cut(s) 784
Eco47I GGWCC 5 cut(s) 342, 758, 1936, 2081, 2207
Eco52I CGGCCG 1 cut(s) 888
Eco53kI GAGCTC 3 cut(s) 1114, 1712, 2280
Eco57I CTGAAG 4 cut(s) 555, 881, 2444, 2468
Eco72I CACGTG 2 cut(s) 2297, 2315
Eco81I CCTNAGG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 2996
EcoICRI GAGCTC 3 cut(s) 1114, 1712, 2280
EcoNI CCTNNNNNAGG 1 cut(s) 2120
EcoO109I RGGNCCY 1 cut(s) 2081
EcoRII CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
EcoT14I CCWWGG 4 cut(s) 609, 1023, 1932, 3051
EcoT38I GRGCYC 4 cut(s) 1031, 1116, 1714, 2282
ErhI CCWWGG 4 cut(s) 609, 1023, 1932, 3051
FaqI GGGAC 4 cut(s) 295, 401, 1913, 2141
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 749
FokI GGATG 5 cut(s) 76, 428, 515, 1550, 1865
FriOI GRGCYC 4 cut(s) 1031, 1116, 1714, 2282
GsuI CTGGAG 4 cut(s) 1101, 1190, 1318, 2604
HaeIII GGCC 8 cut(s) 890, 1286, 1504, 1790, 2021, 2060, 2582, 3002
HapII CCGG 5 cut(s) 756, 1060, 1567, 1907, 2061
HgaI GACGC 2 cut(s) 398, 3030
Hin1I GRCGYC 3 cut(s) 390, 1926, 3041
HincII GTYRAC 1 cut(s) 1426
HindII GTYRAC 1 cut(s) 1426
HpaII CCGG 5 cut(s) 756, 1060, 1567, 1907, 2061
HphI GGTGA 8 cut(s) 748, 903, 1393, 1738, 1934, 2270, 2509, 2558
Hpy166II GTNNAC 6 cut(s) 592, 750, 911, 1426, 2554, 2795
Hpy8I GTNNAC 6 cut(s) 592, 750, 911, 1426, 2554, 2795
HpyCH4III ACNGT 5 cut(s) 652, 703, 1987, 2050, 3013
HpyCH4IV ACGT 4 cut(s) 1926, 2296, 2314, 2797
HpyF3I CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
HpySE526I ACGT 4 cut(s) 1926, 2296, 2314, 2797
Hsp92I GRCGYC 3 cut(s) 390, 1926, 3041
Kpn2I TCCGGA 2 cut(s) 1059, 1566
Kzo9I GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
LguI GCTCTTC 1 cut(s) 1565
LweI GCATC 2 cut(s) 262, 1210
MaeII ACGT 4 cut(s) 1926, 2296, 2314, 2797
MalI GATC 6 cut(s) 867, 1324, 1536, 2457, 2467, 2571
MboI GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
MfeI CAATTG 1 cut(s) 2751
MflI RGATCY 3 cut(s) 865, 1322, 2465
MhlI GDGCHC 6 cut(s) 1031, 1116, 1714, 1951, 2282, 2502
MlyI GAGTC 8 cut(s) 458, 755, 1197, 1881, 2318, 2330, 2715, 3087
MroI TCCGGA 2 cut(s) 1059, 1566
MslI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
MspA1I CMGCKG 2 cut(s) 659, 3059
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 5 cut(s) 756, 1060, 1567, 1907, 2061
MspR9I CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2622
MunI CAATTG 1 cut(s) 2751
Mva1269I GAATGC 1 cut(s) 2847
MvaI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
NciI CCSGG 1 cut(s) 756
NcoI CCATGG 2 cut(s) 1932, 3051
NdeII GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
NlaIV GGNNCC 4 cut(s) 343, 1058, 2113, 2209
NmeAIII GCCGAG 1 cut(s) 2378
NmuCI GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
NspI RCATGY 2 cut(s) 25, 1270
NspV TTCGAA 1 cut(s) 2860
PaeI GCATGC 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 2996
PaqCI CACCTGC 1 cut(s) 2485
PceI AGGCCT 2 cut(s) 1504, 3002
PciSI GCTCTTC 1 cut(s) 1565
PctI GAATGC 1 cut(s) 2847
PfeI GAWTC 9 cut(s) 245, 410, 980, 1044, 1307, 1767, 2604, 2662, 2894
PflMI CCANNNNNTGG 1 cut(s) 1646
PfoI TCCNGGA 1 cut(s) 1681
PleI GAGTC 8 cut(s) 457, 754, 1197, 1880, 2317, 2329, 2715, 3086
PmaCI CACGTG 2 cut(s) 2297, 2315
PmlI CACGTG 2 cut(s) 2297, 2315
PpsI GAGTC 8 cut(s) 457, 754, 1197, 1880, 2317, 2329, 2715, 3086
Ppu21I YACGTR 2 cut(s) 2297, 2315
PpuMI RGGWCCY 1 cut(s) 2081
PshBI ATTAAT 1 cut(s) 1302
Psp124BI GAGCTC 3 cut(s) 1116, 1714, 2282
Psp5II RGGWCCY 1 cut(s) 2081
Psp6I CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
PspCI CACGTG 2 cut(s) 2297, 2315
PspGI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
PspN4I GGNNCC 4 cut(s) 343, 1058, 2113, 2209
PspPI GGNCC 5 cut(s) 342, 758, 1936, 2081, 2207
PspPPI RGGWCCY 1 cut(s) 2081
PspXI VCTCGAGB 1 cut(s) 2996
PstI CTGCAG 4 cut(s) 2395, 2496, 2764, 3064
PstNI CAGNNNCTG 2 cut(s) 1229, 2627
PsuI RGATCY 3 cut(s) 865, 1322, 2465
PvuII CAGCTG 2 cut(s) 659, 3059
RsaI GTAC 6 cut(s) 300, 514, 607, 1331, 2163, 2703
RsaNI GTAC 6 cut(s) 299, 513, 606, 1330, 2162, 2702
RseI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
SacI GAGCTC 3 cut(s) 1116, 1714, 2282
SapI GCTCTTC 1 cut(s) 1565
Sau3AI GATC 6 cut(s) 865, 1322, 1534, 2455, 2465, 2569
Sau96I GGNCC 5 cut(s) 342, 758, 1936, 2081, 2207
SbfI CCTGCAGG 1 cut(s) 2496
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 8 cut(s) 458, 755, 1197, 1881, 2318, 2330, 2715, 3087
ScrFI CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2622
SdaI CCTGCAGG 1 cut(s) 2496
SduI GDGCHC 6 cut(s) 1031, 1116, 1714, 1951, 2282, 2502
SfaNI GCATC 2 cut(s) 262, 1210
SfcI CTRYAG 9 cut(s) 253, 822, 852, 1893, 2391, 2492, 2726, 2760, 3060
Sfr274I CTCGAG 1 cut(s) 2996
SfuI TTCGAA 1 cut(s) 2860
SinI GGWCC 5 cut(s) 342, 758, 1936, 2081, 2207
SlaI CTCGAG 1 cut(s) 2996
SmiMI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
SmlI CTYRAG 4 cut(s) 302, 993, 2505, 2996
SmoI CTYRAG 4 cut(s) 302, 993, 2505, 2996
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 25
Sse8387I CCTGCAGG 1 cut(s) 2496
SseBI AGGCCT 2 cut(s) 1504, 3002
SsiI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 3025
SstI GAGCTC 3 cut(s) 1116, 1714, 2282
StuI AGGCCT 2 cut(s) 1504, 3002
StyD4I CCNGG 8 cut(s) 754, 970, 1036, 1078, 1206, 1334, 1681, 2620
StyI CCWWGG 4 cut(s) 609, 1023, 1932, 3051
TaaI ACNGT 5 cut(s) 652, 703, 1987, 2050, 3013
TaiI ACGT 4 cut(s) 1929, 2299, 2317, 2800
TaqI TCGA 6 cut(s) 596, 978, 1470, 2860, 2997, 3081
TaqII GACCGA 1 cut(s) 184
TatI WGTACW 3 cut(s) 298, 512, 1329
TauI GCSGC 2 cut(s) 1199, 2582
TfiI GAWTC 9 cut(s) 245, 410, 980, 1044, 1307, 1767, 2604, 2662, 2894
TscAI CASTG 6 cut(s) 910, 1123, 2203, 2791, 2797, 3069
TseFI GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
Tsp45I GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
TspDTI ATGAA 7 cut(s) 78, 1320, 1488, 2277, 2349, 2835, 2867
TspRI CASTG 6 cut(s) 910, 1123, 2203, 2791, 2797, 3069
Van91I CCANNNNNTGG 1 cut(s) 1646
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 5 cut(s) 342, 758, 1936, 2081, 2207
VspI ATTAAT 1 cut(s) 1302
XagI CCTNNNNNAGG 1 cut(s) 2120
XapI RAATTY 4 cut(s) 188, 953, 1314, 2264
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 2 cut(s) 25, 1270
XhoI CTCGAG 1 cut(s) 2996
XmiI GTMKAC 1 cut(s) 749
ZraI GACGTC 1 cut(s) 1927
ZrmI AGTACT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.