Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0011012

Physical Location & Seq

Genomic Coordinates Reverse (-)

34507535 .. 34514239

Gene Structure 6705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1213700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3075 bp

ATGCAGGGAACAATGGCTTCTTTGGTTTCCCTGGTTAGTTGGGGAGGTCTTACAATTGCGGGTTCATCCGAAAGGTCACGCTTGCTTGTTAGGAAAGTTTCTTTGCCTAAGACAAGTTTCAAGGGTAGCAGGAGATGGCATTGTCTAAGGTTATCCGTGTGTAAATATTCGGTCACAACTACTGATTTTGTTGCCGAGCAAGGCAATGAGGTATCCCTTGATTCTAACGACTACGGAGGAACTAAAGTTGTTTCTGATGCCAGTGCTGATTTTGTACTTAAGCCCAGTCCGAAGCCAGTATTGAAGTCCTCAGGGGGTTCCAGTACTGAACCCCTTTTAGGTATTGATGCAGCTGACTGGGACCCCTCAAGAATTAGTGGGGATTCAGATGAAGAAAACGAAGAAGAGAGAAATACGGTGATTGAATCGCTTGGAGAGGTTTTGGAGAAGGCTGAAAAGTTAGAAACTGCAAGAGTGGGTGAACTGGGTACTAAGACGGATAGTGGCTCTGTAAATAAACGTGCACCATCTAATGCAAGTGTTAATTTGCGAAATACTAAACCAGTGGACACGGCGGCAACTAGTAAGTCTAAAACTTTGAAAAGTGTGTGGCGTAAAGGAGACACGGTCGCAACTGTGAAAAAGGTTGTAAAGGAATCACCTAAAGTTAGCAATACAATTCAGAATGAGGAGACAAAGAAGGGAGGAGAGGTAAAGGTAGAGTCTCAACCCCGTGCTCCTTTAAGAACCCCTCAGCCCCCCTTGAGACCTCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACAGTGAAGAAACCTGTTGTGTTGAAGGATGTGGGGGCAGCTCCGAAGTCGTCAGAGATTGATGAAGCTGATTCGTCCACAAAAACTAAAGAACGGAAGGGACCAATTTTGATTGACAAATTTGCTTCCAAAAAGGCAGTGGTCGATTCTGTGATTTCTCAAGCAGTTTTAGCTCCTTCAAAACCAGGAAAGGGCCCTTCCCCTGGAAAGGTCAAAGAAGGGTTTCGAAAGAAGGATGCACCTGGACCACGGAGGCGGAAAGTTGCTGAGGATATTCTGGACGAAGACACGTCAGAACTCAATGTCTCTATTCCTGGAGCAGCAAGGAAAGGGAGGAAGTGGAATAAAGCAAGCAGGAAGGCAGCGAGACTTCAGGCTGAGAAAGAGGCGGCTCCTGTTAAAGTCGAAATTCTAGAGGTCGGTGAAGATGGTATGTTGGTAGACGATTTGGCGTTCCATTTGGCAACAACTGAATCCGAAATTCTTGGATATCTGTACTCTATGGGAATTAAACCAGATGGGGTTCAAACTCTGGACAAAGATATGGTAAAGATGATTTGTAAAGAGTATGTCGTGGAAGTTATAGATGTTGATCCTGTGAAGGTGGAAGAAATGGCAAGAAAGAAGGAAATACTTGATGAAGATGACCTAGACAAACTACAAGACAGACCTCCCGTCCTCACTATAATGGGTCATGTGGATCATGGCAAGACTACCCTGTTGGATCATATCCGGAAGAGCAAGGTTGCTGCCTCAGAGGCTGGTGGAATTACACAAGGAATTGGGGCATATAAGGTGCTTGTACCAATTGATGGAAAGTTGCGATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGTGCAATGAGAGCTCGTGGAACAAGGGTGACAGACATTGCTATCATTGTAGTGGCAGCTGATGATGGAATCCGCCCGCAAACAAAAGAGGCCATAGCTCATGCTAAAGCTGCTGGAGTGCCAATTGTAATTGCTATAAATAAGATAGACAAGGATGGAGCTAATCCAGATCGAGTCATGCAAGAGCTTTCTTCTATCGGCCTAATGCCAGAGGACTGGGGTGGTGACATCCCAATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGAGCTTTTGGAAACTGTTATGCTAGTTGCAGAGTTGCAAGATTTAAAGGCAAATCCTCATAGAAGTGCAAAAGGCTCAGTCATTGAGGCCGGTCTTCATAAATCTAAGGGACCTGTAGTTACACTTATAGTGCAAAATGGAACCCTCAAACAAGGGGACATAGTAGTTTGTGGAGGAGCATTCGGAAAGGTGCGTGCTTTGTTTGATGATGGTGGAAATCGTGTCGATGAAGCTGGACCCTCTATACCTGTTCAGGTTCTTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAAGTTGTCAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTTGCGTGCTGAGTCATTGCGAAATGAACGTATATCAGCTAAGGCTGGCGATGGCAGGGTTACTCTTTCTTCCTTAGCTTCTGCAGTCTCAGCAGGAAAGCTGTCTGGAATAGACTTGCACCAGCTAAATATTATTCTGAAGGTTGATCTTCAGGGATCCATCGAGGCCGTCAGACAAGCTCTGCAGGTGCTTCCACAAGATAATGTCACCTTGAAATTTCTCTTGGAAACAACAGGCGATGTAAGCACCAGCGATATTGATCTTGCTGCTGCTAGCAAGGCAATAATTTTTGGATTCAATGTCAAAGTACCAGGATCTGTAAAAAGCTATGGAGATAATAAAGGGGTTGAGATTCGCCTATATAGAGTTATATATGAACTCATTGATGATGTAAGAAATGCAATGGAAGGACTCCTAGAGCCTGTTGAGGAACGAGTAACAATTGGATCAGCAGAAGTCCGGGCCATATTCAGCTCTGGTAGTGGTCGTGTTGCTGGATGCATGATAAATGAGGGAAAAGTTGTTAAAGGCTGTGGCGTGGAGGTCATTCGAAGGGGAAAAGTAGTACATGTTGGTCTTGTTGATTCATTGAAACGGGTCAAGGAAGTTGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTACGACGACTGGGAGGAGGGAGATAGCCTTGAGTTCTTCAACACAGTTCAAAAGAAGCGGACACTTGAGGAGGCTTCCGCTTCAATGGCAGCTGCAGTGGAAGGAACAGGAAGAAAGGTGCAAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GTP_EFTU IF-2 IF2_N

Protein Analysis

1025

Amino Acids

109.67

Weight (kDa)

6.53

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	498 - 658	5.6e-35	Elongation factor Tu GTP binding domain
MMR_HSR1	PF01926	499 - 607	1.6e-08	50S ribosome-binding GTPase
EF-G_D2	PF22042	673 - 751	1.1e-24	Elongation factor G domain 2
IF-2	PF11987	801 - 895	3.8e-30	Translation-initiation factor 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17220
fragaria_vesca	FvH4_3g16100 FvH4_3g16100 FvH4_3g16100
malus_domestica	MD05G1213700.v1.1 MD10G1197600.v1.1
prunus_persica	Prupe.4G144300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0027081
rosa_laevigata	RLG00000032988
rosa_multiflora	Rmu_co8113306.1_g000001 Rmu_sc0000882.1_g000026 Rmu_sc0006065.1_g000025 Rmu_sc0031914.1_g000001
rosa_roxburghii	Rroxscaffold_1G00052410
rosa_rugosa	Rorug05G0097200
rosa_samantha	Rh5AG190800 Rh5BG188400 Rh5CG208200 Rh5DG190200
rosa_wichuraiana	Rw5G017380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2464
Acc36I	ACCTGC	1 cut(s)	2464
AccB7I	CCANNNNNTGG	1 cut(s)	1625
AccI	GTMKAC	1 cut(s)	1254
AccIII	TCCGGA	1 cut(s)	1545
AciI	CCGC	8 cut(s)	59, 575, 1069, 1202, 1750, 1754, 3004, 3024
AclWI	GGATC	7 cut(s)	1400, 1521, 1545, 2439, 2452, 2611, 2743
AcsI	RAATTY	5 cut(s)	932, 1221, 1293, 2243, 2504
AcuI	CTGAAG	3 cut(s)	1169, 2423, 2447
AcvI	CACGTG	1 cut(s)	2276
AfaI	GTAC	7 cut(s)	276, 325, 490, 1310, 1617, 2598, 2856
AfiI	CCNNNNNNNGG	6 cut(s)	338, 1004, 1016, 1021, 1625, 2234
AflII	CTTAAG	1 cut(s)	278
AflIII	ACRYGT	2 cut(s)	1101, 2857
AhdI	GACNNNNNGTC	1 cut(s)	1487
AhlI	ACTAGT	1 cut(s)	581
AjiI	CACGTC	1 cut(s)	1104
AjnI	CCWGG	7 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599
AjuI	GAANNNNNNNTTGG	2 cut(s)	2495, 2527
Alw21I	GWGCWC	4 cut(s)	526, 739, 1693, 1930
Alw26I	GTCTC	7 cut(s)	615, 686, 729, 760, 1123, 1174, 2380
Alw44I	GTGCAC	1 cut(s)	522
AlwI	GGATC	7 cut(s)	1400, 1521, 1545, 2439, 2452, 2611, 2743
AlwNI	CAGNNNCTG	2 cut(s)	1574, 2606
Aor13HI	TCCGGA	1 cut(s)	1545
AoxI	GGCC	6 cut(s)	1006, 1767, 1876, 2037, 2454, 2751
ApaI	GGGCCC	1 cut(s)	1010
ApaLI	GTGCAC	1 cut(s)	522
ApoI	RAATTY	5 cut(s)	932, 1221, 1293, 2243, 2504
Asp700I	GAANNNNTTC	1 cut(s)	1035
AspS9I	GGNCC	9 cut(s)	361, 914, 1006, 1007, 1058, 1915, 2060, 2186, 2751
AsuC2I	CCSGG	1 cut(s)	2750
AsuHPI	GGTGA	8 cut(s)	430, 491, 651, 1247, 1717, 1913, 2249, 2488
AsuII	TTCGAA	2 cut(s)	1039, 2839
AsuNHI	GCTAGC	1 cut(s)	2561
AvaII	GGWCC	6 cut(s)	361, 914, 1058, 1915, 2060, 2186
AxyI	CCTNAGG	1 cut(s)	310
BaeGI	GKGCMC	2 cut(s)	526, 1010
BamHI	GGATCC	1 cut(s)	2444
BanII	GRGCYC	2 cut(s)	1010, 1693
BauI	CACGAG	1 cut(s)	1692
BbrPI	CACGTG	1 cut(s)	2276
BbsI	GAAGAC	3 cut(s)	1104, 2036, 2949
Bbv12I	GWGCWC	4 cut(s)	526, 739, 1693, 1930
BbvCI	CCTCAGC	2 cut(s)	753, 1080
BceAI	ACGGC	2 cut(s)	588, 2441
BciT130I	CCWGG	7 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601
BciVI	GTATCC	1 cut(s)	223
BcnI	CCSGG	1 cut(s)	2750
BcoDI	GTCTC	7 cut(s)	615, 686, 729, 760, 1123, 1174, 2380
BcuI	ACTAGT	1 cut(s)	581
BfaI	CTAG	7 cut(s)	582, 1226, 1463, 1973, 2562, 2705, 2921
BfmI	CTRYAG	5 cut(s)	798, 2064, 2370, 2471, 3039
BfrI	CTTAAG	1 cut(s)	278
BfuAI	ACCTGC	1 cut(s)	2464
BfuI	GTATCC	1 cut(s)	223
BglI	GCCNNNNNGGC	1 cut(s)	1571
BmcAI	AGTACT	1 cut(s)	325
Bme1390I	CCNGG	8 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601, 2750
Bme18I	GGWCC	6 cut(s)	361, 914, 1058, 1915, 2060, 2186
BmeRI	GACNNNNNGTC	1 cut(s)	1487
BmgBI	CACGTC	1 cut(s)	1104
BmgT120I	GGNCC	9 cut(s)	361, 914, 1006, 1007, 1058, 1915, 2060, 2186, 2751
BmrFI	CCNGG	8 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601, 2750
BmrI	ACTGGG	5 cut(s)	279, 367, 494, 1903, 2965
BmsI	GCATC	4 cut(s)	247, 337, 1039, 2777
BmtI	GCTAGC	1 cut(s)	2565
BmuI	ACTGGG	5 cut(s)	279, 367, 494, 1903, 2965
BpiI	GAAGAC	3 cut(s)	1104, 2036, 2949
BpmI	CTGGAG	2 cut(s)	1149, 1812
Bpu10I	CCTNAGC	4 cut(s)	753, 1080, 2328, 2362
Bpu14I	TTCGAA	2 cut(s)	1039, 2839
BpuEI	CTTGAG	5 cut(s)	352, 784, 957, 2996, 3032
BpuMI	CCSGG	1 cut(s)	2750
BsaAI	YACGTR	1 cut(s)	2276
BsaBI	GATNNNNATC	2 cut(s)	1404, 2547
BsaI	GGTCTC	1 cut(s)	760
BsaJI	CCNNGG	3 cut(s)	30, 1015, 1061
BsaWI	WCCGGW	1 cut(s)	1545
BsaXI	ACNNNNNCTCC	6 cut(s)	612, 642, 696, 726, 790, 820
Bsc4I	CCNNNNNNNGG	6 cut(s)	338, 1004, 1016, 1021, 1625, 2234
Bse118I	RCCGGY	1 cut(s)	2039
Bse1I	ACTGG	9 cut(s)	261, 285, 296, 321, 362, 489, 563, 1898, 2960
Bse21I	CCTNAGG	1 cut(s)	310
Bse3DI	GCAATG	5 cut(s)	211, 1689, 1713, 2303, 2697
Bse8I	GATNNNNATC	2 cut(s)	1404, 2547
BseAI	TCCGGA	1 cut(s)	1545
BseBI	CCWGG	7 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601
BseDI	CCNNGG	3 cut(s)	30, 1015, 1061
BseGI	GGATG	6 cut(s)	65, 847, 1054, 1837, 1905, 2792
BseJI	GATNNNNATC	2 cut(s)	1404, 2547
BseLI	CCNNNNNNNGG	6 cut(s)	338, 1004, 1016, 1021, 1625, 2234
BseMI	GCAATG	5 cut(s)	211, 1689, 1713, 2303, 2697
BseMII	CTCAG	8 cut(s)	324, 767, 1071, 1182, 1581, 2040, 2289, 2391
BseNI	ACTGG	9 cut(s)	261, 285, 296, 321, 362, 489, 563, 1898, 2960
BseRI	GAGGAG	6 cut(s)	704, 720, 795, 2139, 2975, 3029
BseSI	GKGCMC	2 cut(s)	526, 1010
Bsh1285I	CGRYCG	1 cut(s)	630
BshFI	GGCC	6 cut(s)	1008, 1769, 1878, 2039, 2456, 2753
BsiEI	CGRYCG	1 cut(s)	630
BsiHKAI	GWGCWC	4 cut(s)	526, 739, 1693, 1930
BsiSI	CCGG	3 cut(s)	1546, 2040, 2749
BslFI	GGGAC	4 cut(s)	374, 927, 2073, 2120
BslI	CCNNNNNNNGG	6 cut(s)	338, 1004, 1016, 1021, 1625, 2234
BsmAI	GTCTC	7 cut(s)	615, 686, 729, 760, 1123, 1174, 2380
BsmFI	GGGAC	4 cut(s)	374, 927, 2073, 2120
BsmI	GAATGC	1 cut(s)	2129
BsnI	GGCC	6 cut(s)	1008, 1769, 1878, 2039, 2456, 2753
Bso31I	GGTCTC	1 cut(s)	760
Bsp119I	TTCGAA	2 cut(s)	1039, 2839
Bsp120I	GGGCCC	1 cut(s)	1006
Bsp1286I	GDGCHC	5 cut(s)	526, 739, 1010, 1693, 1930
Bsp13I	TCCGGA	1 cut(s)	1545
BspACI	CCGC	8 cut(s)	59, 575, 1069, 1202, 1750, 1754, 3004, 3024
BspANI	GGCC	6 cut(s)	1008, 1769, 1878, 2039, 2456, 2753
BspCNI	CTCAG	8 cut(s)	323, 766, 1072, 1183, 1580, 2039, 2290, 2390
BspEI	TCCGGA	1 cut(s)	1545
BspMAI	CTGCAG	3 cut(s)	2374, 2475, 3043
BspMI	ACCTGC	1 cut(s)	2464
BspOI	GCTAGC	1 cut(s)	2565
BspPI	GGATC	7 cut(s)	1400, 1521, 1545, 2439, 2452, 2611, 2743
BspQI	GCTCTTC	1 cut(s)	1544
BspT104I	TTCGAA	2 cut(s)	1039, 2839
BspTI	CTTAAG	1 cut(s)	278
BspTNI	GGTCTC	1 cut(s)	760
BsrDI	GCAATG	5 cut(s)	211, 1689, 1713, 2303, 2697
BsrFI	RCCGGY	1 cut(s)	2039
BsrI	ACTGG	9 cut(s)	261, 285, 296, 321, 362, 489, 563, 1898, 2960
BssAI	RCCGGY	1 cut(s)	2039
BssECI	CCNNGG	3 cut(s)	30, 1015, 1061
BssSI	CACGAG	1 cut(s)	1692
Bst2BI	CACGAG	1 cut(s)	1692
Bst2UI	CCWGG	7 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601
Bst4CI	ACNGT	6 cut(s)	418, 628, 637, 817, 1966, 2992
Bst6I	CTCTTC	2 cut(s)	399, 1544
BstAFI	CTTAAG	1 cut(s)	278
BstAPI	GCANNNNNTGC	1 cut(s)	1679
BstBAI	YACGTR	1 cut(s)	2276
BstBI	TTCGAA	2 cut(s)	1039, 2839
BstC8I	GCNNGC	7 cut(s)	83, 1165, 1754, 2145, 2295, 2335, 2563
BstDSI	CCRYGG	1 cut(s)	1061
BstF5I	GGATG	6 cut(s)	65, 847, 1054, 1837, 1905, 2792
BstMAI	GTCTC	7 cut(s)	615, 686, 729, 760, 1123, 1174, 2380
BstMCI	CGRYCG	1 cut(s)	630
BstMWI	GCNNNNNNNGC	9 cut(s)	983, 1571, 1679, 1688, 1787, 2378, 2532, 2567, 3032
BstNI	CCWGG	7 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601
BstNSI	RCATGY	1 cut(s)	2861
BstSCI	CCNGG	8 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599, 2748
BstSFI	CTRYAG	5 cut(s)	798, 2064, 2370, 2471, 3039
BstSLI	GKGCMC	2 cut(s)	526, 1010
BstV2I	GAAGAC	3 cut(s)	1104, 2036, 2949
BstX2I	RGATCY	2 cut(s)	2444, 2603
BstXI	CCANNNNNNTGG	1 cut(s)	1893
BstYI	RGATCY	2 cut(s)	2444, 2603
Bsu36I	CCTNAGG	1 cut(s)	310
BsuI	GTATCC	1 cut(s)	223
BsuRI	GGCC	6 cut(s)	1008, 1769, 1878, 2039, 2456, 2753
BtgI	CCRYGG	1 cut(s)	1061
BtgZI	GCGATG	2 cut(s)	2352, 2541
BtrI	CACGTC	1 cut(s)	1104
BtsCI	GGATG	6 cut(s)	65, 847, 1054, 1837, 1905, 2792
BtsI	GCAGTG	2 cut(s)	957, 3048
BtsIMutI	CAGTG	5 cut(s)	268, 570, 822, 957, 3048
BveI	ACCTGC	1 cut(s)	2464
Cac8I	GCNNGC	7 cut(s)	83, 1165, 1754, 2145, 2295, 2335, 2563
CaiI	CAGNNNCTG	2 cut(s)	1574, 2606
Cfr10I	RCCGGY	1 cut(s)	2039
Cfr13I	GGNCC	9 cut(s)	361, 914, 1006, 1007, 1058, 1915, 2060, 2186, 2751
Csp6I	GTAC	7 cut(s)	275, 324, 489, 1309, 1616, 2597, 2855
CviAII	CATG	8 cut(s)	1508, 1517, 1641, 1667, 1778, 1855, 2791, 2858
CviQI	GTAC	7 cut(s)	275, 324, 489, 1309, 1616, 2597, 2855
DraI	TTTAAA	1 cut(s)	1995
DriI	GACNNNNNGTC	1 cut(s)	1487
Eam1104I	CTCTTC	2 cut(s)	399, 1544
Eam1105I	GACNNNNNGTC	1 cut(s)	1487
EarI	CTCTTC	2 cut(s)	399, 1544
EciI	GGCGGA	2 cut(s)	1084, 1739
Ecl136II	GAGCTC	1 cut(s)	1691
Eco24I	GRGCYC	2 cut(s)	1010, 1693
Eco31I	GGTCTC	1 cut(s)	760
Eco32I	GATATC	1 cut(s)	1304
Eco47I	GGWCC	6 cut(s)	361, 914, 1058, 1915, 2060, 2186
Eco53kI	GAGCTC	1 cut(s)	1691
Eco57I	CTGAAG	3 cut(s)	1169, 2423, 2447
Eco72I	CACGTG	1 cut(s)	2276
Eco81I	CCTNAGG	1 cut(s)	310
EcoICRI	GAGCTC	1 cut(s)	1691
EcoO109I	RGGNCCY	4 cut(s)	361, 1006, 1007, 2060
EcoRII	CCWGG	7 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599
EcoRV	GATATC	1 cut(s)	1304
EcoT22I	ATGCAT	1 cut(s)	2792
EcoT38I	GRGCYC	2 cut(s)	1010, 1693
FaeI	CATG	8 cut(s)	1511, 1520, 1644, 1670, 1781, 1858, 2794, 2861
FaqI	GGGAC	4 cut(s)	374, 927, 2073, 2120
FatI	CATG	8 cut(s)	1507, 1516, 1640, 1666, 1777, 1854, 2790, 2857
FauI	CCCGC	2 cut(s)	52, 1761
FblI	GTMKAC	1 cut(s)	1254
FokI	GGATG	6 cut(s)	52, 854, 1061, 1844, 1892, 2799
FriOI	GRGCYC	2 cut(s)	1010, 1693
FspBI	CTAG	7 cut(s)	582, 1226, 1463, 1973, 2562, 2705, 2921
GsuI	CTGGAG	2 cut(s)	1149, 1812
HaeIII	GGCC	6 cut(s)	1008, 1769, 1878, 2039, 2456, 2753
HapII	CCGG	3 cut(s)	1546, 2040, 2749
Hin1II	CATG	8 cut(s)	1511, 1520, 1644, 1670, 1781, 1858, 2794, 2861
HpaII	CCGG	3 cut(s)	1546, 2040, 2749
HphI	GGTGA	8 cut(s)	430, 491, 651, 1247, 1717, 1913, 2249, 2488
Hpy166II	GTNNAC	5 cut(s)	482, 524, 568, 891, 1255
Hpy188III	TCNNGA	8 cut(s)	369, 1091, 1226, 1346, 1546, 1844, 1918, 2394
Hpy8I	GTNNAC	5 cut(s)	482, 524, 568, 891, 1255
Hpy99I	CGWCG	1 cut(s)	2954
HpyCH4III	ACNGT	6 cut(s)	418, 628, 637, 817, 1966, 2992
HpyCH4IV	ACGT	4 cut(s)	520, 1103, 2275, 2317
HpyF10VI	GCNNNNNNNGC	9 cut(s)	983, 1571, 1679, 1688, 1787, 2378, 2532, 2567, 3032
HpySE526I	ACGT	4 cut(s)	520, 1103, 2275, 2317
Hsp92II	CATG	8 cut(s)	1511, 1520, 1644, 1670, 1781, 1858, 2794, 2861
KflI	GGGWCCC	1 cut(s)	361
Kpn2I	TCCGGA	1 cut(s)	1545
LguI	GCTCTTC	1 cut(s)	1544
LmnI	GCTCC	9 cut(s)	742, 808, 859, 991, 1130, 1210, 1835, 2126, 2264
LweI	GCATC	4 cut(s)	247, 337, 1039, 2777
MaeI	CTAG	7 cut(s)	582, 1226, 1463, 1973, 2562, 2705, 2921
MaeII	ACGT	4 cut(s)	520, 1103, 2275, 2317
MaeIII	GTNAC	8 cut(s)	75, 172, 1705, 1901, 2068, 2347, 2494, 2725
MfeI	CAATTG	4 cut(s)	54, 1620, 1800, 2730
MflI	RGATCY	2 cut(s)	2444, 2603
MhlI	GDGCHC	5 cut(s)	526, 739, 1010, 1693, 1930
MlyI	GAGTC	4 cut(s)	731, 1860, 2309, 2694
MmeI	TCCRAC	1 cut(s)	1515
Mph1103I	ATGCAT	1 cut(s)	2792
MroI	TCCGGA	1 cut(s)	1545
MroXI	GAANNNNTTC	1 cut(s)	1035
MseI	TTAA	9 cut(s)	279, 543, 743, 1212, 1323, 1932, 1994, 2216, 2814
MslI	CAYNNNNRTG	4 cut(s)	1499, 1727, 1910, 2013
MspA1I	CMGCKG	3 cut(s)	353, 1736, 3038
MspCI	CTTAAG	1 cut(s)	278
MspI	CCGG	3 cut(s)	1546, 2040, 2749
MspR9I	CCNGG	8 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601, 2750
MunI	CAATTG	4 cut(s)	54, 1620, 1800, 2730
Mva1269I	GAATGC	1 cut(s)	2129
MvaI	CCWGG	7 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601
MwoI	GCNNNNNNNGC	9 cut(s)	983, 1571, 1679, 1688, 1787, 2378, 2532, 2567, 3032
NciI	CCSGG	1 cut(s)	2750
NheI	GCTAGC	1 cut(s)	2561
NlaIII	CATG	8 cut(s)	1511, 1520, 1644, 1670, 1781, 1858, 2794, 2861
NmeAIII	GCCGAG	1 cut(s)	220
NmuCI	GTSAC	5 cut(s)	75, 172, 1705, 1901, 2494
NsiI	ATGCAT	1 cut(s)	2792
NspI	RCATGY	1 cut(s)	2861
NspV	TTCGAA	2 cut(s)	1039, 2839
PaqCI	CACCTGC	1 cut(s)	2464
PciI	ACATGT	1 cut(s)	2857
PciSI	GCTCTTC	1 cut(s)	1544
PctI	GAATGC	1 cut(s)	2129
PdmI	GAANNNNTTC	1 cut(s)	1035
PflFI	GACNNNGTC	1 cut(s)	626
PflMI	CCANNNNNTGG	1 cut(s)	1625
PfoI	TCCNGGA	2 cut(s)	1126, 1660
PleI	GAGTC	4 cut(s)	730, 1859, 2308, 2694
PmaCI	CACGTG	1 cut(s)	2276
PmlI	CACGTG	1 cut(s)	2276
PpsI	GAGTC	4 cut(s)	730, 1859, 2308, 2694
Ppu21I	YACGTR	1 cut(s)	2276
PpuMI	RGGWCCY	2 cut(s)	361, 2060
PscI	ACATGT	1 cut(s)	2857
Psp124BI	GAGCTC	1 cut(s)	1693
Psp5II	RGGWCCY	2 cut(s)	361, 2060
Psp6I	CCWGG	7 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599
PspCI	CACGTG	1 cut(s)	2276
PspGI	CCWGG	7 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599
PspOMI	GGGCCC	1 cut(s)	1006
PspPI	GGNCC	9 cut(s)	361, 914, 1006, 1007, 1058, 1915, 2060, 2186, 2751
PspPPI	RGGWCCY	2 cut(s)	361, 2060
PstI	CTGCAG	3 cut(s)	2374, 2475, 3043
PstNI	CAGNNNCTG	2 cut(s)	1574, 2606
PsuI	RGATCY	2 cut(s)	2444, 2603
PsyI	GACNNNGTC	1 cut(s)	626
PvuII	CAGCTG	3 cut(s)	353, 1736, 3038
RsaI	GTAC	7 cut(s)	276, 325, 490, 1310, 1617, 2598, 2856
RsaNI	GTAC	7 cut(s)	275, 324, 489, 1309, 1616, 2597, 2855
RseI	CAYNNNNRTG	4 cut(s)	1499, 1727, 1910, 2013
SacI	GAGCTC	1 cut(s)	1693
SapI	GCTCTTC	1 cut(s)	1544
SaqAI	TTAA	9 cut(s)	279, 543, 743, 1212, 1323, 1932, 1994, 2216, 2814
Sau96I	GGNCC	9 cut(s)	361, 914, 1006, 1007, 1058, 1915, 2060, 2186, 2751
ScaI	AGTACT	1 cut(s)	325
SchI	GAGTC	4 cut(s)	731, 1860, 2309, 2694
ScrFI	CCNGG	8 cut(s)	32, 999, 1017, 1056, 1128, 1662, 2601, 2750
SduI	GDGCHC	5 cut(s)	526, 739, 1010, 1693, 1930
SfaNI	GCATC	4 cut(s)	247, 337, 1039, 2777
SfcI	CTRYAG	5 cut(s)	798, 2064, 2370, 2471, 3039
SfuI	TTCGAA	2 cut(s)	1039, 2839
SinI	GGWCC	6 cut(s)	361, 914, 1058, 1915, 2060, 2186
SmiMI	CAYNNNNRTG	4 cut(s)	1499, 1727, 1910, 2013
SmlI	CTYRAG	6 cut(s)	278, 367, 763, 972, 2975, 3011
SmoI	CTYRAG	6 cut(s)	278, 367, 763, 972, 2975, 3011
SpeI	ACTAGT	1 cut(s)	581
SsiI	CCGC	8 cut(s)	59, 575, 1069, 1202, 1750, 1754, 3004, 3024
SspI	AATATT	2 cut(s)	167, 2419
SspMI	CTAG	7 cut(s)	582, 1226, 1463, 1973, 2562, 2705, 2921
SstI	GAGCTC	1 cut(s)	1693
StyD4I	CCNGG	8 cut(s)	30, 997, 1015, 1054, 1126, 1660, 2599, 2748
TaaI	ACNGT	6 cut(s)	418, 628, 637, 817, 1966, 2992
TaiI	ACGT	4 cut(s)	523, 1106, 2278, 2320
TaqI	TCGA	7 cut(s)	957, 1039, 1218, 1849, 2175, 2451, 2839
TaqII	GACCGA	1 cut(s)	160
TatI	WGTACW	4 cut(s)	274, 323, 1308, 2854
TauI	GCSGC	2 cut(s)	578, 1205
Tru1I	TTAA	9 cut(s)	279, 543, 743, 1212, 1323, 1932, 1994, 2216, 2814
Tru9I	TTAA	9 cut(s)	279, 543, 743, 1212, 1323, 1932, 1994, 2216, 2814
TscAI	CASTG	5 cut(s)	268, 570, 822, 957, 3048
TseFI	GTSAC	5 cut(s)	75, 172, 1705, 1901, 2494
Tsp45I	GTSAC	5 cut(s)	75, 172, 1705, 1901, 2494
TspGWI	ACGGA	5 cut(s)	145, 249, 512, 922, 1078
TspRI	CASTG	5 cut(s)	268, 570, 822, 957, 3048
Tth111I	GACNNNGTC	1 cut(s)	626
Van91I	CCANNNNNTGG	1 cut(s)	1625
Vha464I	CTTAAG	1 cut(s)	278
VneI	GTGCAC	1 cut(s)	522
VpaK11BI	GGWCC	6 cut(s)	361, 914, 1058, 1915, 2060, 2186
XapI	RAATTY	5 cut(s)	932, 1221, 1293, 2243, 2504
XbaI	TCTAGA	1 cut(s)	1225
XceI	RCATGY	1 cut(s)	2861
XcmI	CCANNNNNNNNNTGG	1 cut(s)	949
XmiI	GTMKAC	1 cut(s)	1254
XmnI	GAANNNNTTC	1 cut(s)	1035
XspI	CTAG	7 cut(s)	582, 1226, 1463, 1973, 2562, 2705, 2921
ZrmI	AGTACT	1 cut(s)	325
Zsp2I	ATGCAT	1 cut(s)	2792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.