MD10G1197600.v1.1

Translation initiation factor IF-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011012
Physical Location & Seq
Reverse (-)
29575850 .. 29582470
6621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1197600.v1.1.491

Sequence Viewer

Length: 3093 bp
ATGCTAGTTTTAGTAGGAAGCATGCAGGAAACAATGGCTTCTTTGGTTTCCCTTGTTAGTTGGGGAGGTCTTACACTTGCGGGTTCATCCGAAAGGTCCGGCTTGCTTGTTAGGAAAGTTTCTTTGTCTAAGACAAGTTTTAAGGGTAGTAGGAGATGGCATTGTCTAAGGTTATCAGTGTGTAAATATTCGGTCACAACTACTGATTTTGTTGCCGAGCAAGGCAATGAGGTGTCCCTTGATTCTAACAACTATAGAGGAACTAAAGTTGTTTCTGATGCCAATGCTGATTTTGTACTTAAGCCCGGTCCAAAGCCAGTTTTGAAGTCCTCAGGGGGGTCCAGTACTGAACCCCTTTTAGGTATTGATGCAGCTGACTGGGACCCCTCAAGAATTAGCGGGGATTCGGATGAAGAAGGTGAAGAAGAGAGAAATAAGGTGATTGAGTCGCTTGGAGAGGTTTTGGAGAAGGCTGAAAAGTTAGAAACTGCAAGGGTGGGTGAACTGGGTACTAAGAAAGATAGTGCCTCTGTAAATAAACCTGCACCATCCAGTACAAGTAATAATTTGCGAAATGCTACACCAGTGGACTTAGCGGCAACTAGTAAGTCTAAAACTTTGAAGAGTGTGTGGCGTAAAGGAGACACGGTCTCAACTGTGAAAAAGGTTGTAAAGGAATCACCTAAAGTTAATAATACAATTCAGAAAGAGGAAACAAAAACAGGAGGAGGGGTACAGGTAGAGTCTCAACCCCGTGCTCCTTTAAGAACCCCTCAGCCCCCCTTGAGACCTCAGCCAACATTACAAGCAAAGCCTTCTACAGCTCTTCCACCAACGGTGAAGAAACCTGTTGTGTTGAAAGATGTGGGGGCCGCTGCAAAGTCTTCAGTGATTGATGAAACCGATTTGTCCACAAAAACTAAAGAACGGAAGGCACCAATTTTGATTGACAAATTTGCTTCCAAAAAGGCGTCGGTTGATTCTGTGATTTCTCAAGCAGTTTTAGCTCCTTCAAAACCAGGAAAGGGCCCTTCCCCTGGAAAGTTCAAAGAAGGGTTTCGGAAGAAAGACGCAGCTGGACTACGGAGGCGGAAGGTTGCTGATGATATTCTGGATGAAGACACTTCGGAACTCAATGTCTCTATTCCTGGAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCAGCGAGACTTCAGGCTGCGAAAGAGGCGGCTCCTGTTAAAGTAGAAATTCTAGAGGTCGGTGAAGATGGTATGTTGGTAGACGATTTGGCATTCCATTTGGCTACAACTGAATCCGAAATTCTTGGATATCTGTACTCGATGGGAATTAAGCCGGATGGGGTTCAAACTCTGGACAAAGATATGGTAAAGATGATTTGTAAAGAGTACGACGTGGAAGTTATAGATGTTGATCCTGTGAAGGTGGAAGAAATGGCAAGAAAGAAGGAATTACTTGATGTAGATGACCTAGACAAACTACAAGACAGACCTCCTGTCCTCACTATAATGGGTCATGTGGATCATGGCAAGACAACCTTGTTGGACCATATCCGGAAGAGCAAGGTTGCTGCCTCAGAGGCTGGTGGAATTACACAAGGAATTGGGGCATATAAGGTGCTTGTGCCAATTGATGGAAAGTTGCAATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGTGCAATGAGAGCTCGTGGAACAAGGGTGACAGACATTGCTATCATTGTAGTGGCAGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATAGCTCATGCTAAAGCTGCTGGAGTGCCAATAGTAATTGCTATAAATAAGATAGACAAGGATGGAGCCAATCCAGATCGAGTCATGCAAGAGCTTTCTTATATTGGTCTAATGCCAGAGAGGACTGGGGTGGCGACGTCCCAATGGTCCAGTGCTCTTAAAGGGAAGAATATAAATGAGCTTTTGGAAACTGTTATGCTAGTTGCAGAGTTGCAAGATTTAAAGGCAAATCCCCATAGAAGTGCGAAAGGCTCAGTCATTGAGGCCGGTCTTCATAAATCTAAAGGACCTGTAGTTACACTTATAGTGCAAAATGGAACCCTCAAACAAGGGGACATAGTAGTTTGTGGAGGAGCATTCGGAAAGGTGCGGGCCTTGTTTGATGATGGTGGAAATCGTGTCAATGAAGCTGGGCCCTCTATACCTGTTCAGGTTCTGGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAAGTTGTCAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTTGCGTGCTGAGTCATTGCGAAATGAACGTATATCAGCTAAGGCTGGCGATGGCAGGGTTACTCTTTCTTCCTTAGCTTCTGCAGTCTCAGCAGGAAAGCTGTCTGGACTAGACTTGCACCAGCTAAATATTATTCTGAAGGTTGATCTTCAGGGATCCATCGAGGCTGTCAGACAAGCCCTGCAGGTGCTTCCACAAGATAATGTCACCTTGAAGTTTCTCTTGGAAACAACAGGCGATGTAAGCAGCAGTGATGTTGATCTTGCTGCTGCTAGCAAAGCCATAATTTTTGGATTCAATGTCAAAATACCAGGATCTGTAAAAAGCTATGGTGATAGCAAAGGGGTTGAGATTCGCTTATATAGAGTTATATATGAACTTATTGATGATGTACGAAATGCAATGGAAGGACTTCTAGAGCCTGTTGAGGAACGAGTAACAATTGGATCAGCAGAAGTCCGAGCCGTATTCAGCTCTGGTAGTGGTCGTGTTGCTGGATGCATGATAAATGAGGGAAAAGTTGTTAAAGGCTGTGGCGTTGAGGTCATTCGAAGGGGAAAAGTCGTACATGTTGGCCTTCTTGATTCATTGAAACGGGTCAAGGAAGTTGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGAGTGGAAGACTACGACGAGTGGGAGGAGGGAGATACTCTTGAGTTCTTCAACACAGTTCAAAAGAAGCGGACACTAGAAGAGGCGTCTGCTTCAATGGCAGCTGCAGTGGAAGGAACAGGAAGAAAGGTGCAAGATTGA

Protein Analysis

1031

Amino Acids

110.15

Weight (kDa)

7.87

Isoelectric Point (pI)

33.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF2_N PF04760 425 - 471 3.8e-06 Translation initiation factor IF-2, N-terminal region
GTP_EFTU PF00009 505 - 664 1.4e-31 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 506 - 614 1.7e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 679 - 757 1.6e-24 Elongation factor G domain 2
IF-2 PF11987 807 - 901 5e-30 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2482
AatII GACGTC 1 cut(s) 1931
Acc36I ACCTGC 2 cut(s) 550, 2482
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccI GTMKAC 1 cut(s) 1275
AccIII TCCGGA 1 cut(s) 1566
AciI CCGC 9 cut(s) 80, 399, 596, 873, 1090, 1223, 1771, 2161, 3022
AclWI GGATC 6 cut(s) 1421, 1542, 2457, 2470, 2629, 2761
AcsI RAATTY 4 cut(s) 953, 1242, 1314, 2261
AcuI CTGAAG 4 cut(s) 870, 1190, 2441, 2465
AcvI CACGTG 1 cut(s) 2294
AcyI GRCGYC 3 cut(s) 971, 1928, 3038
AfaI GTAC 9 cut(s) 297, 346, 511, 556, 735, 1331, 1403, 2700, 2874
AfiI CCNNNNNNNGG 6 cut(s) 336, 359, 1025, 1037, 1646, 2252
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 1 cut(s) 2875
AhdI GACNNNNNGTC 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 602
AjiI CACGTC 1 cut(s) 1408
AjnI CCWGG 5 cut(s) 1018, 1036, 1147, 1681, 2617
AjuI GAANNNNNNNTTGG 2 cut(s) 2513, 2545
Alw21I GWGCWC 3 cut(s) 760, 1714, 1948
Alw26I GTCTC 7 cut(s) 636, 655, 750, 781, 1144, 1195, 2398
AlwI GGATC 6 cut(s) 1421, 1542, 2457, 2470, 2629, 2761
AlwNI CAGNNNCTG 3 cut(s) 1595, 2227, 2624
Aor13HI TCCGGA 1 cut(s) 1566
AoxI GGCC 7 cut(s) 870, 1027, 1788, 2055, 2163, 2204, 2881
ApaI GGGCCC 2 cut(s) 1031, 2208
ApoI RAATTY 4 cut(s) 953, 1242, 1314, 2261
Asp700I GAANNNNTTC 2 cut(s) 1056, 2718
AsuC2I CCSGG 1 cut(s) 306
AsuII TTCGAA 1 cut(s) 2857
AsuNHI GCTAGC 1 cut(s) 2579
AvaII GGWCC 7 cut(s) 96, 308, 339, 382, 1558, 1938, 2078
AxyI CCTNAGG 1 cut(s) 331
BaeGI GKGCMC 2 cut(s) 1031, 2208
BamHI GGATCC 1 cut(s) 2462
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 3 cut(s) 1031, 1714, 2208
BauI CACGAG 1 cut(s) 1713
BbrPI CACGTG 1 cut(s) 2294
BbsI GAAGAC 4 cut(s) 876, 1125, 2054, 2967
Bbv12I GWGCWC 3 cut(s) 760, 1714, 1948
BbvCI CCTCAGC 2 cut(s) 774, 792
BceAI ACGGC 1 cut(s) 2756
BciT130I CCWGG 5 cut(s) 1020, 1038, 1149, 1683, 2619
BcnI CCSGG 1 cut(s) 306
BcoDI GTCTC 7 cut(s) 636, 655, 750, 781, 1144, 1195, 2398
BcuI ACTAGT 1 cut(s) 602
BfmI CTRYAG 6 cut(s) 253, 819, 2082, 2388, 2489, 3057
BfrI CTTAAG 1 cut(s) 299
BfuAI ACCTGC 2 cut(s) 550, 2482
BglI GCCNNNNNGGC 1 cut(s) 1592
BmcAI AGTACT 1 cut(s) 346
Bme1390I CCNGG 6 cut(s) 306, 1020, 1038, 1149, 1683, 2619
Bme18I GGWCC 7 cut(s) 96, 308, 339, 382, 1558, 1938, 2078
BmeRI GACNNNNNGTC 1 cut(s) 1508
BmgBI CACGTC 1 cut(s) 1408
BmrFI CCNGG 6 cut(s) 306, 1020, 1038, 1149, 1683, 2619
BmrI ACTGGG 3 cut(s) 388, 515, 1926
BmsI GCATC 3 cut(s) 268, 358, 2795
BmtI GCTAGC 1 cut(s) 2583
BmuI ACTGGG 3 cut(s) 388, 515, 1926
BpiI GAAGAC 4 cut(s) 876, 1125, 2054, 2967
BpmI CTGGAG 2 cut(s) 1170, 1833
Bpu10I CCTNAGC 4 cut(s) 774, 792, 2346, 2380
Bpu14I TTCGAA 1 cut(s) 2857
BpuEI CTTGAG 4 cut(s) 373, 805, 978, 3014
BpuMI CCSGG 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 2294
BsaBI GATNNNNATC 2 cut(s) 1425, 2565
BsaHI GRCGYC 3 cut(s) 971, 1928, 3038
BsaI GGTCTC 2 cut(s) 655, 781
BsaJI CCNNGG 1 cut(s) 1036
BsaWI WCCGGW 1 cut(s) 1566
BsaXI ACNNNNNCTCC 4 cut(s) 633, 663, 717, 747
Bsc4I CCNNNNNNNGG 6 cut(s) 336, 359, 1025, 1037, 1646, 2252
Bse118I RCCGGY 1 cut(s) 2057
Bse1I ACTGG 8 cut(s) 317, 342, 383, 510, 552, 584, 1921, 1941
Bse21I CCTNAGG 1 cut(s) 331
Bse3DI GCAATG 5 cut(s) 232, 1710, 1734, 2321, 2715
Bse8I GATNNNNATC 2 cut(s) 1425, 2565
BseAI TCCGGA 1 cut(s) 1566
BseBI CCWGG 5 cut(s) 1020, 1038, 1149, 1683, 2619
BseDI CCNNGG 1 cut(s) 1036
BseGI GGATG 7 cut(s) 86, 415, 548, 1120, 1357, 1858, 2810
BseJI GATNNNNATC 2 cut(s) 1425, 2565
BseLI CCNNNNNNNGG 6 cut(s) 336, 359, 1025, 1037, 1646, 2252
BseMI GCAATG 5 cut(s) 232, 1710, 1734, 2321, 2715
BseMII CTCAG 7 cut(s) 345, 788, 806, 1602, 2058, 2307, 2409
BseNI ACTGG 8 cut(s) 317, 342, 383, 510, 552, 584, 1921, 1941
BseRI GAGGAG 3 cut(s) 741, 2157, 2993
BseSI GKGCMC 2 cut(s) 1031, 2208
BseYI CCCAGC 1 cut(s) 2201
BsgI GTGCAG 1 cut(s) 528
BshFI GGCC 7 cut(s) 872, 1029, 1790, 2057, 2165, 2206, 2883
BshNI GGYRCC 1 cut(s) 934
BsiHKAI GWGCWC 3 cut(s) 760, 1714, 1948
BsiSI CCGG 5 cut(s) 99, 306, 1349, 1567, 2058
BslFI GGGAC 4 cut(s) 220, 395, 1915, 2138
BslI CCNNNNNNNGG 6 cut(s) 336, 359, 1025, 1037, 1646, 2252
BsmAI GTCTC 7 cut(s) 636, 655, 750, 781, 1144, 1195, 2398
BsmFI GGGAC 4 cut(s) 220, 395, 1915, 2138
BsmI GAATGC 2 cut(s) 1286, 2147
BsnI GGCC 7 cut(s) 872, 1029, 1790, 2057, 2165, 2206, 2883
Bso31I GGTCTC 2 cut(s) 655, 781
Bsp119I TTCGAA 1 cut(s) 2857
Bsp120I GGGCCC 2 cut(s) 1027, 2204
Bsp1286I GDGCHC 5 cut(s) 760, 1031, 1714, 1948, 2208
Bsp13I TCCGGA 1 cut(s) 1566
Bsp143I GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
BspACI CCGC 9 cut(s) 80, 399, 596, 873, 1090, 1223, 1771, 2161, 3022
BspANI GGCC 7 cut(s) 872, 1029, 1790, 2057, 2165, 2206, 2883
BspCNI CTCAG 7 cut(s) 344, 787, 805, 1601, 2057, 2308, 2408
BspEI TCCGGA 1 cut(s) 1566
BspMAI CTGCAG 3 cut(s) 2392, 2493, 3061
BspMI ACCTGC 2 cut(s) 550, 2482
BspOI GCTAGC 1 cut(s) 2583
BspPI GGATC 6 cut(s) 1421, 1542, 2457, 2470, 2629, 2761
BspQI GCTCTTC 2 cut(s) 831, 1565
BspT104I TTCGAA 1 cut(s) 2857
BspT107I GGYRCC 1 cut(s) 934
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 2 cut(s) 655, 781
BsrDI GCAATG 5 cut(s) 232, 1710, 1734, 2321, 2715
BsrFI RCCGGY 1 cut(s) 2057
BsrI ACTGG 8 cut(s) 317, 342, 383, 510, 552, 584, 1921, 1941
BssAI RCCGGY 1 cut(s) 2057
BssECI CCNNGG 1 cut(s) 1036
BssMI GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
BssNI GRCGYC 3 cut(s) 971, 1928, 3038
BssSI CACGAG 1 cut(s) 1713
Bst2BI CACGAG 1 cut(s) 1713
Bst2UI CCWGG 5 cut(s) 1020, 1038, 1149, 1683, 2619
Bst4CI ACNGT 5 cut(s) 649, 658, 838, 1984, 3010
Bst6I CTCTTC 5 cut(s) 420, 617, 831, 1565, 3027
BstACI GRCGYC 3 cut(s) 971, 1928, 3038
BstAFI CTTAAG 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 1700
BstBAI YACGTR 1 cut(s) 2294
BstBI TTCGAA 1 cut(s) 2857
BstC8I GCNNGC 7 cut(s) 23, 104, 1186, 2163, 2313, 2353, 2581
BstF5I GGATG 7 cut(s) 86, 415, 548, 1120, 1357, 1858, 2810
BstKTI GATC 8 cut(s) 1429, 1537, 1870, 2455, 2465, 2569, 2624, 2756
BstMAI GTCTC 7 cut(s) 636, 655, 750, 781, 1144, 1195, 2398
BstMBI GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
BstNI CCWGG 5 cut(s) 1020, 1038, 1149, 1683, 2619
BstNSI RCATGY 2 cut(s) 25, 2879
BstSCI CCNGG 6 cut(s) 304, 1018, 1036, 1147, 1681, 2617
BstSFI CTRYAG 6 cut(s) 253, 819, 2082, 2388, 2489, 3057
BstSLI GKGCMC 2 cut(s) 1031, 2208
BstV2I GAAGAC 4 cut(s) 876, 1125, 2054, 2967
BstX2I RGATCY 2 cut(s) 2462, 2621
BstYI RGATCY 2 cut(s) 2462, 2621
Bsu36I CCTNAGG 1 cut(s) 331
BsuRI GGCC 7 cut(s) 872, 1029, 1790, 2057, 2165, 2206, 2883
BtgZI GCGATG 2 cut(s) 2370, 2559
BtrI CACGTC 1 cut(s) 1408
BtsCI GGATG 7 cut(s) 86, 415, 548, 1120, 1357, 1858, 2810
BtsI GCAGTG 2 cut(s) 2563, 3066
BtsIMutI CAGTG 6 cut(s) 183, 591, 894, 1948, 2563, 3066
BveI ACCTGC 2 cut(s) 550, 2482
Cac8I GCNNGC 7 cut(s) 23, 104, 1186, 2163, 2313, 2353, 2581
CaiI CAGNNNCTG 3 cut(s) 1595, 2227, 2624
Cfr10I RCCGGY 1 cut(s) 2057
CseI GACGC 3 cut(s) 960, 1079, 3027
Csp6I GTAC 9 cut(s) 296, 345, 510, 555, 734, 1330, 1402, 2699, 2873
CviAII CATG 9 cut(s) 22, 1529, 1538, 1662, 1688, 1799, 1876, 2809, 2876
CviQI GTAC 9 cut(s) 296, 345, 510, 555, 734, 1330, 1402, 2699, 2873
DpnI GATC 8 cut(s) 1428, 1536, 1869, 2454, 2464, 2568, 2623, 2755
DpnII GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
DraI TTTAAA 1 cut(s) 2013
DriI GACNNNNNGTC 1 cut(s) 1508
Eam1104I CTCTTC 5 cut(s) 420, 617, 831, 1565, 3027
Eam1105I GACNNNNNGTC 1 cut(s) 1508
EarI CTCTTC 5 cut(s) 420, 617, 831, 1565, 3027
EciI GGCGGA 2 cut(s) 1105, 1760
Ecl136II GAGCTC 1 cut(s) 1712
Eco24I GRGCYC 3 cut(s) 1031, 1714, 2208
Eco31I GGTCTC 2 cut(s) 655, 781
Eco32I GATATC 1 cut(s) 1325
Eco47I GGWCC 7 cut(s) 96, 308, 339, 382, 1558, 1938, 2078
Eco53kI GAGCTC 1 cut(s) 1712
Eco57I CTGAAG 4 cut(s) 870, 1190, 2441, 2465
Eco72I CACGTG 1 cut(s) 2294
Eco81I CCTNAGG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 1712
EcoO109I RGGNCCY 5 cut(s) 382, 1027, 1028, 2078, 2205
EcoRII CCWGG 5 cut(s) 1018, 1036, 1147, 1681, 2617
EcoRV GATATC 1 cut(s) 1325
EcoT22I ATGCAT 1 cut(s) 2810
EcoT38I GRGCYC 3 cut(s) 1031, 1714, 2208
FaeI CATG 9 cut(s) 25, 1532, 1541, 1665, 1691, 1802, 1879, 2812, 2879
FalI AAGNNNNNCTT 4 cut(s) 1535, 1567, 2513, 2545
FaqI GGGAC 4 cut(s) 220, 395, 1915, 2138
FatI CATG 9 cut(s) 21, 1528, 1537, 1661, 1687, 1798, 1875, 2808, 2875
FauI CCCGC 3 cut(s) 73, 392, 2154
FblI GTMKAC 1 cut(s) 1275
FokI GGATG 7 cut(s) 73, 422, 535, 1127, 1364, 1865, 2817
FriOI GRGCYC 3 cut(s) 1031, 1714, 2208
GsaI CCCAGC 1 cut(s) 2205
GsuI CTGGAG 2 cut(s) 1170, 1833
HaeIII GGCC 7 cut(s) 872, 1029, 1790, 2057, 2165, 2206, 2883
HapII CCGG 5 cut(s) 99, 306, 1349, 1567, 2058
HgaI GACGC 3 cut(s) 960, 1079, 3027
Hin1I GRCGYC 3 cut(s) 971, 1928, 3038
Hin1II CATG 9 cut(s) 25, 1532, 1541, 1665, 1691, 1802, 1879, 2812, 2879
HpaII CCGG 5 cut(s) 99, 306, 1349, 1567, 2058
Hpy166II GTNNAC 4 cut(s) 503, 589, 912, 1276
Hpy8I GTNNAC 4 cut(s) 503, 589, 912, 1276
Hpy99I CGWCG 4 cut(s) 976, 1409, 1930, 2972
HpyCH4III ACNGT 5 cut(s) 649, 658, 838, 1984, 3010
HpyCH4IV ACGT 4 cut(s) 1407, 1928, 2293, 2335
HpySE526I ACGT 4 cut(s) 1407, 1928, 2293, 2335
Hsp92I GRCGYC 3 cut(s) 971, 1928, 3038
Hsp92II CATG 9 cut(s) 25, 1532, 1541, 1665, 1691, 1802, 1879, 2812, 2879
KflI GGGWCCC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 1566
Kzo9I GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
LguI GCTCTTC 2 cut(s) 831, 1565
LmnI GCTCC 7 cut(s) 763, 1012, 1151, 1231, 1856, 2144, 2282
LweI GCATC 3 cut(s) 268, 358, 2795
MaeII ACGT 4 cut(s) 1407, 1928, 2293, 2335
MaeIII GTNAC 6 cut(s) 193, 1726, 2086, 2365, 2512, 2743
MalI GATC 8 cut(s) 1428, 1536, 1869, 2454, 2464, 2568, 2623, 2755
MboI GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
MfeI CAATTG 2 cut(s) 1641, 2748
MflI RGATCY 2 cut(s) 2462, 2621
MhlI GDGCHC 5 cut(s) 760, 1031, 1714, 1948, 2208
MlyI GAGTC 4 cut(s) 455, 752, 1881, 2327
MmeI TCCRAC 1 cut(s) 1536
Mph1103I ATGCAT 1 cut(s) 2810
MroI TCCGGA 1 cut(s) 1566
MroXI GAANNNNTTC 2 cut(s) 1056, 2718
MslI CAYNNNNRTG 3 cut(s) 1520, 1748, 2031
MspA1I CMGCKG 5 cut(s) 374, 875, 1076, 1757, 3056
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 5 cut(s) 99, 306, 1349, 1567, 2058
MspR9I CCNGG 6 cut(s) 306, 1020, 1038, 1149, 1683, 2619
MunI CAATTG 2 cut(s) 1641, 2748
Mva1269I GAATGC 2 cut(s) 1286, 2147
MvaI CCWGG 5 cut(s) 1020, 1038, 1149, 1683, 2619
NciI CCSGG 1 cut(s) 306
NdeII GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
NheI GCTAGC 1 cut(s) 2579
NlaIII CATG 9 cut(s) 25, 1532, 1541, 1665, 1691, 1802, 1879, 2812, 2879
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 3 cut(s) 193, 1726, 2512
NsiI ATGCAT 1 cut(s) 2810
NspI RCATGY 2 cut(s) 25, 2879
NspV TTCGAA 1 cut(s) 2857
PaeI GCATGC 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 2482
PciI ACATGT 1 cut(s) 2875
PciSI GCTCTTC 2 cut(s) 831, 1565
PctI GAATGC 2 cut(s) 1286, 2147
PdmI GAANNNNTTC 2 cut(s) 1056, 2718
PfeI GAWTC 9 cut(s) 242, 404, 677, 980, 1307, 1767, 2601, 2659, 2891
PflFI GACNNNGTC 1 cut(s) 647
PflMI CCANNNNNTGG 1 cut(s) 1646
PfoI TCCNGGA 2 cut(s) 1147, 1681
PleI GAGTC 4 cut(s) 454, 751, 1880, 2326
PmaCI CACGTG 1 cut(s) 2294
PmlI CACGTG 1 cut(s) 2294
PpsI GAGTC 4 cut(s) 454, 751, 1880, 2326
Ppu21I YACGTR 1 cut(s) 2294
PpuMI RGGWCCY 2 cut(s) 382, 2078
PscI ACATGT 1 cut(s) 2875
Psp124BI GAGCTC 1 cut(s) 1714
Psp5II RGGWCCY 2 cut(s) 382, 2078
Psp6I CCWGG 5 cut(s) 1018, 1036, 1147, 1681, 2617
PspCI CACGTG 1 cut(s) 2294
PspFI CCCAGC 1 cut(s) 2201
PspGI CCWGG 5 cut(s) 1018, 1036, 1147, 1681, 2617
PspOMI GGGCCC 2 cut(s) 1027, 2204
PspPPI RGGWCCY 2 cut(s) 382, 2078
PstI CTGCAG 3 cut(s) 2392, 2493, 3061
PstNI CAGNNNCTG 3 cut(s) 1595, 2227, 2624
PsuI RGATCY 2 cut(s) 2462, 2621
PsyI GACNNNGTC 1 cut(s) 647
PvuII CAGCTG 4 cut(s) 374, 1076, 1757, 3056
RsaI GTAC 9 cut(s) 297, 346, 511, 556, 735, 1331, 1403, 2700, 2874
RsaNI GTAC 9 cut(s) 296, 345, 510, 555, 734, 1330, 1402, 2699, 2873
RseI CAYNNNNRTG 3 cut(s) 1520, 1748, 2031
SacI GAGCTC 1 cut(s) 1714
SapI GCTCTTC 2 cut(s) 831, 1565
Sau3AI GATC 8 cut(s) 1426, 1534, 1867, 2452, 2462, 2566, 2621, 2753
SbfI CCTGCAGG 1 cut(s) 2493
ScaI AGTACT 1 cut(s) 346
SchI GAGTC 4 cut(s) 455, 752, 1881, 2327
ScrFI CCNGG 6 cut(s) 306, 1020, 1038, 1149, 1683, 2619
SdaI CCTGCAGG 1 cut(s) 2493
SduI GDGCHC 5 cut(s) 760, 1031, 1714, 1948, 2208
SfaNI GCATC 3 cut(s) 268, 358, 2795
SfcI CTRYAG 6 cut(s) 253, 819, 2082, 2388, 2489, 3057
SfuI TTCGAA 1 cut(s) 2857
SinI GGWCC 7 cut(s) 96, 308, 339, 382, 1558, 1938, 2078
SmiMI CAYNNNNRTG 3 cut(s) 1520, 1748, 2031
SmlI CTYRAG 5 cut(s) 299, 388, 784, 993, 2993
SmoI CTYRAG 5 cut(s) 299, 388, 784, 993, 2993
SpeI ACTAGT 1 cut(s) 602
SphI GCATGC 1 cut(s) 25
Sse8387I CCTGCAGG 1 cut(s) 2493
SsiI CCGC 9 cut(s) 80, 399, 596, 873, 1090, 1223, 1771, 2161, 3022
SspI AATATT 2 cut(s) 188, 2437
SstI GAGCTC 1 cut(s) 1714
StyD4I CCNGG 6 cut(s) 304, 1018, 1036, 1147, 1681, 2617
TaaI ACNGT 5 cut(s) 649, 658, 838, 1984, 3010
TaiI ACGT 4 cut(s) 1410, 1931, 2296, 2338
TaqI TCGA 4 cut(s) 1334, 1870, 2469, 2857
TaqII GACCGA 1 cut(s) 181
TatI WGTACW 4 cut(s) 295, 344, 554, 1329
TauI GCSGC 3 cut(s) 599, 875, 1226
TfiI GAWTC 9 cut(s) 242, 404, 677, 980, 1307, 1767, 2601, 2659, 2891
TscAI CASTG 6 cut(s) 183, 591, 894, 1948, 2563, 3066
TseFI GTSAC 3 cut(s) 193, 1726, 2512
Tsp45I GTSAC 3 cut(s) 193, 1726, 2512
TspGWI ACGGA 2 cut(s) 943, 1099
TspRI CASTG 6 cut(s) 183, 591, 894, 1948, 2563, 3066
Tth111I GACNNNGTC 1 cut(s) 647
Van91I CCANNNNNTGG 1 cut(s) 1646
Vha464I CTTAAG 1 cut(s) 299
VpaK11BI GGWCC 7 cut(s) 96, 308, 339, 382, 1558, 1938, 2078
XapI RAATTY 4 cut(s) 953, 1242, 1314, 2261
XbaI TCTAGA 2 cut(s) 1246, 2722
XceI RCATGY 2 cut(s) 25, 2879
XmiI GTMKAC 1 cut(s) 1275
XmnI GAANNNNTTC 2 cut(s) 1056, 2718
ZraI GACGTC 1 cut(s) 1929
ZrmI AGTACT 1 cut(s) 346
Zsp2I ATGCAT 1 cut(s) 2810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.