Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011012

Physical Location & Seq

Genomic Coordinates Forward (+)

21767359 .. 21774158

Gene Structure 6800 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032988

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2967 bp

ATGGTTTCTTTGGCTTCCCTTGTTAGTTTGGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTTTTAGGAAGGTTTCGTTGTCTAAGAGTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGATTGTCAGTTTGTAAATTTTCCGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTATCCCTTGATTCTAACTATAGAGGAAGTAGTAATGATGCTTCTGTTGCCAATGCTGATTTTGTACTTAAGCCGTCCCCGAAGCCAGTATTGAAGCCCTCTGAGGGGTCCAATGCTGAACCGCCCCTTTTAAGCCTTAATGCTGCTGATTGGGATGCCTCAAAAACTAGTGGGGATTTGGATGTGGAAGAGGAAGATAAGAGTAATGTGATTGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTAGAAGCTCCAAAAGTGGGTGATTCGAGTACTAAGAAAGCCAGTAAGCCTGTAAATAGACCTGCACCTTCTAATGCAACTACTACTTCAGGAAATGCTAGACCAGTAAACTCGACAGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGTAAAGGAGACACAGTTGCAGCTGTGCGAAAGGTTGTAAAGGAATTACCTAAGGTTAATAATACAGTTCGGAGAGAAGAACCAAAAACAGGGGAAGGGGTGAAGGTAGAATCGCCAACCCGTCCTCCCTTAAGACCTCCTTCTCCACCTTTGAGACCCCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTCTGAAGGATCTTGGGGCAGCTCCAAAGTCGGCAGTGATTGATGACACTGGTTCACCCCCAAAAACTAAAGAAAGGAAGCCAATTTTGATTGACAAATTTTCTACCAAGAAGCCAGGGGTCGATTCTGTGGTTTCTCAAGCAGTTTTAGCTCCTACAAAACCAGCCAAGGGCTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCTGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTCACTGATGATGAGAGTTCGGAACTCAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCAAGACTCCAGGCTGCCAAAGATGCAGCTCCTGTTAAAGTAGATATTCTAGAGGTTGATGACGATGGCATGTTAATAGATGAGTTGGCCTTCAATTTGGCTATTAATGAATCCGAAATTCTCGGATCTCTGTACTCCAGGGGAATTAAGCCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTAAAGATGATTTGTAAAGAGTATGATGTGGAAGTCATAGATGTTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTCGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTTACTATAATGGGTCATGTGGATCATGGCAAGACAACTCTATTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACACAAGGAATTGGGGCATACAAGGTGCTTGTGCCAGTTGATGGAAAGTTGCAATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGAGCAATGAGAGCTCGTGGAGCAAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCGATAGTAATAGCTATAAATAAGATAGACAAGGATGGAGCTAATCCAGAACGAGTCATGCAAGAGCTTTCTTCTATTGGTCTAATGCCGGAGGATTGGGGTGGTGACGTCCCAATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTTTTGGAAACCGTAATGCTAGTTGCAGAGATGTGTAATCCTACTATCATCATAATAGTTTGTCTTAATTTACCTTCCTGTAACCTCTCTAGGCTTGTACGGGCTTTATTTGATGATGGTGGAAATCGTGTCAATGAAGCTGGACCCTCTATACCAGTTCAGGTTATTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAGGTCGTGAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTCACGTGCAGAGTCATTGCGAAATGAACGCATATCAGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCTTTAGCTTCTGCAGTCTCGGCAGGAAAGCTGGCTGGACTTGACTTGCACCAGCTAAATGTTATTCTGAAGGTTGATCTTCAGGGATCTATCGAGGCTGTCAGACAAGCCCTGCAGGTGCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACACCAGCGATGTTGATCTTGCTGCGGCCAGCAAAGCCATAATTTTGGGATTCAATATCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAAGGTGTGGAGATTCGGCTATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGACGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTTAAAGGCTGCGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTACATGTTGGCGTTCTTGATTCTTTAAAACGGGTTAAGGAAGTCGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTATGACGATTTTGAGGAGGGAGACATTCTCGAGGCCTTCAACACAGTCCAAAAGAAGCGTACACTAGAAGAGGCGTCTGCTTCCATGGCTGCTGCAGTGGAAGAAACAGGAGTCGAATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GTP_EFTU IF-2 IF2_N

Protein Analysis

989

Amino Acids

105.65

Weight (kDa)

6.79

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	494 - 654	3.1e-34	Elongation factor Tu GTP binding domain
MMR_HSR1	PF01926	495 - 603	1.9e-08	50S ribosome-binding GTPase
EF-G_D2	PF22042	680 - 717	1.4e-08	Elongation factor G domain 2
IF-2	PF11987	767 - 861	1.6e-31	Translation-initiation factor 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17220
fragaria_vesca	FvH4_3g16100 FvH4_3g16100 FvH4_3g16100
malus_domestica	MD05G1213700.v1.1 MD10G1197600.v1.1
prunus_persica	Prupe.4G144300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0027081
rosa_laevigata	RLG00000032988
rosa_multiflora	Rmu_co8113306.1_g000001 Rmu_sc0000882.1_g000026 Rmu_sc0006065.1_g000025 Rmu_sc0031914.1_g000001
rosa_roxburghii	Rroxscaffold_1G00052410
rosa_rugosa	Rorug05G0097200
rosa_samantha	Rh5AG190800 Rh5BG188400 Rh5CG208200 Rh5DG190200
rosa_wichuraiana	Rw5G017380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2362
AatII	GACGTC	1 cut(s)	1896
Acc36I	ACCTGC	2 cut(s)	520, 2362
AccB7I	CCANNNNNTGG	1 cut(s)	1613
AccIII	TCCGGA	2 cut(s)	1026, 1533
AciI	CCGC	8 cut(s)	50, 323, 1001, 1063, 1163, 1738, 2456, 2721
AclWI	GGATC	4 cut(s)	840, 1297, 1509, 2350
AcoI	YGGCCR	1 cut(s)	2457
AcsI	RAATTY	4 cut(s)	155, 920, 1281, 2141
AcuI	CTGAAG	4 cut(s)	522, 848, 2321, 2345
AcvI	CACGTG	2 cut(s)	2174, 2192
AcyI	GRCGYC	2 cut(s)	1893, 2918
AfaI	GTAC	8 cut(s)	267, 481, 574, 1298, 2040, 2580, 2754, 2905
AfiI	CCNNNNNNNGG	8 cut(s)	305, 306, 467, 692, 992, 1402, 1613, 2132
AflII	CTTAAG	2 cut(s)	269, 733
AflIII	ACRYGT	2 cut(s)	2674, 2755
AgsI	TTSAA	8 cut(s)	295, 592, 1015, 1258, 1328, 2401, 2485, 2884
AhlI	ACTAGT	1 cut(s)	368
AjiI	CACGTC	1 cut(s)	2675
AjnI	CCWGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
Alw21I	GWGCWC	5 cut(s)	1083, 1681, 1918, 2159, 2379
Alw26I	GTCTC	5 cut(s)	606, 751, 1117, 2278, 2859
AlwI	GGATC	4 cut(s)	840, 1297, 1509, 2350
AlwNI	CAGNNNCTG	2 cut(s)	1196, 2504
Ama87I	CYCGRG	1 cut(s)	2873
Aor13HI	TCCGGA	2 cut(s)	1026, 1533
AoxI	GGCC	5 cut(s)	1251, 1469, 1755, 2457, 2877
ApoI	RAATTY	4 cut(s)	155, 920, 1281, 2141
AseI	ATTAAT	1 cut(s)	1269
AspS9I	GGNCC	3 cut(s)	309, 1903, 2084
AsuHPI	GGTGA	8 cut(s)	482, 715, 870, 1705, 1901, 2147, 2386, 2435
AsuII	TTCGAA	1 cut(s)	2737
AvaI	CYCGRG	1 cut(s)	2873
AvaII	GGWCC	3 cut(s)	309, 1903, 2084
AxyI	CCTNAGG	1 cut(s)	654
BanII	GRGCYC	4 cut(s)	998, 1083, 1681, 2159
BarI	GAAGNNNNNNTAC	6 cut(s)	25, 57, 226, 258, 2586, 2618
BauI	CACGAG	1 cut(s)	1680
BbrPI	CACGTG	2 cut(s)	2174, 2192
BbsI	GAAGAC	2 cut(s)	1470, 2847
Bbv12I	GWGCWC	5 cut(s)	1083, 1681, 1918, 2159, 2379
BceAI	ACGGC	1 cut(s)	259
BciT130I	CCWGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
BciVI	GTATCC	1 cut(s)	214
BcoDI	GTCTC	5 cut(s)	606, 751, 1117, 2278, 2859
BcuI	ACTAGT	1 cut(s)	368
BfmI	CTRYAG	8 cut(s)	220, 789, 819, 2268, 2369, 2603, 2637, 2937
BfrI	CTTAAG	2 cut(s)	269, 733
BfuAI	ACCTGC	2 cut(s)	520, 2362
BfuI	GTATCC	1 cut(s)	214
BglI	GCCNNNNNGGC	1 cut(s)	1559
BlpI	GCTNAGC	1 cut(s)	186
BmcAI	AGTACT	1 cut(s)	481
Bme1390I	CCNGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
Bme18I	GGWCC	3 cut(s)	309, 1903, 2084
BmeT110I	CYCGRG	1 cut(s)	2873
BmgBI	CACGTC	1 cut(s)	2675
BmgT120I	GGNCC	3 cut(s)	309, 1903, 2084
BmiI	GGNNCC	3 cut(s)	310, 1025, 2086
BmrFI	CCNGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
BmrI	ACTGGG	1 cut(s)	1391
BmsI	GCATC	3 cut(s)	229, 346, 1177
BmuI	ACTGGG	1 cut(s)	1391
BpiI	GAAGAC	2 cut(s)	1470, 2847
BplI	GAGNNNNNCTC	2 cut(s)	2856, 2888
BpmI	CTGGAG	4 cut(s)	1068, 1157, 1285, 2481
Bpu10I	CCTNAGC	1 cut(s)	2226
Bpu1102I	GCTNAGC	1 cut(s)	186
Bpu14I	TTCGAA	1 cut(s)	2737
BpuEI	CTTGAG	2 cut(s)	945, 2367
BsaAI	YACGTR	2 cut(s)	2174, 2192
BsaBI	GATNNNNATC	1 cut(s)	2445
BsaHI	GRCGYC	2 cut(s)	1893, 2918
BsaI	GGTCTC	1 cut(s)	751
BsaJI	CCNNGG	6 cut(s)	576, 938, 990, 1302, 1557, 2928
BsaWI	WCCGGW	2 cut(s)	1026, 1533
BsaXI	ACNNNNNCTCC	8 cut(s)	603, 633, 712, 742, 781, 811, 1677, 1707
Bsc4I	CCNNNNNNNGG	8 cut(s)	305, 306, 467, 692, 992, 1402, 1613, 2132
Bse1I	ACTGG	7 cut(s)	287, 492, 554, 877, 1397, 1607, 2096
Bse21I	CCTNAGG	1 cut(s)	654
Bse3DI	GCAATG	4 cut(s)	202, 1677, 2201, 2595
Bse8I	GATNNNNATC	1 cut(s)	2445
BseAI	TCCGGA	2 cut(s)	1026, 1533
BseBI	CCWGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
BseDI	CCNNGG	6 cut(s)	576, 938, 990, 1302, 1557, 2928
BseGI	GGATG	5 cut(s)	56, 361, 388, 1530, 1825
BseJI	GATNNNNATC	1 cut(s)	2445
BseLI	CCNNNNNNNGG	8 cut(s)	305, 306, 467, 692, 992, 1402, 1613, 2132
BseMI	GCAATG	4 cut(s)	202, 1677, 2201, 2595
BseMII	CTCAG	3 cut(s)	177, 294, 1325
BseNI	ACTGG	7 cut(s)	287, 492, 554, 877, 1397, 1607, 2096
BseRI	GAGGAG	2 cut(s)	786, 2873
BsgI	GTGCAG	2 cut(s)	498, 2214
BshFI	GGCC	5 cut(s)	1253, 1471, 1757, 2459, 2879
BsiHKAI	GWGCWC	5 cut(s)	1083, 1681, 1918, 2159, 2379
BsiHKCI	CYCGRG	1 cut(s)	2873
BsiSI	CCGG	3 cut(s)	1027, 1534, 1874
BslFI	GGGAC	2 cut(s)	262, 1880
BslI	CCNNNNNNNGG	8 cut(s)	305, 306, 467, 692, 992, 1402, 1613, 2132
BsmAI	GTCTC	5 cut(s)	606, 751, 1117, 2278, 2859
BsmFI	GGGAC	2 cut(s)	262, 1880
BsmI	GAATGC	1 cut(s)	2724
BsnI	GGCC	5 cut(s)	1253, 1471, 1757, 2459, 2879
Bso31I	GGTCTC	1 cut(s)	751
BsoBI	CYCGRG	1 cut(s)	2873
Bsp119I	TTCGAA	1 cut(s)	2737
Bsp1286I	GDGCHC	6 cut(s)	998, 1083, 1681, 1918, 2159, 2379
Bsp13I	TCCGGA	2 cut(s)	1026, 1533
Bsp143I	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
Bsp1720I	GCTNAGC	1 cut(s)	186
Bsp19I	CCATGG	1 cut(s)	2928
BspACI	CCGC	8 cut(s)	50, 323, 1001, 1063, 1163, 1738, 2456, 2721
BspANI	GGCC	5 cut(s)	1253, 1471, 1757, 2459, 2879
BspCNI	CTCAG	3 cut(s)	178, 295, 1326
BspEI	TCCGGA	2 cut(s)	1026, 1533
BspLI	GGNNCC	3 cut(s)	310, 1025, 2086
BspMAI	CTGCAG	4 cut(s)	2272, 2373, 2641, 2941
BspMI	ACCTGC	2 cut(s)	520, 2362
BspPI	GGATC	4 cut(s)	840, 1297, 1509, 2350
BspQI	GCTCTTC	1 cut(s)	1532
BspT104I	TTCGAA	1 cut(s)	2737
BspTI	CTTAAG	2 cut(s)	269, 733
BspTNI	GGTCTC	1 cut(s)	751
BsrDI	GCAATG	4 cut(s)	202, 1677, 2201, 2595
BsrI	ACTGG	7 cut(s)	287, 492, 554, 877, 1397, 1607, 2096
BssECI	CCNNGG	6 cut(s)	576, 938, 990, 1302, 1557, 2928
BssMI	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
BssNI	GRCGYC	2 cut(s)	1893, 2918
BssSI	CACGAG	1 cut(s)	1680
BssT1I	CCWWGG	3 cut(s)	576, 990, 2928
Bst2BI	CACGAG	1 cut(s)	1680
Bst2UI	CCWGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
Bst4CI	ACNGT	4 cut(s)	619, 670, 1954, 2890
Bst6I	CTCTTC	4 cut(s)	384, 587, 1532, 2907
BstACI	GRCGYC	2 cut(s)	1893, 2918
BstAFI	CTTAAG	2 cut(s)	269, 733
BstBAI	YACGTR	2 cut(s)	2174, 2192
BstBI	TTCGAA	1 cut(s)	2737
BstC8I	GCNNGC	6 cut(s)	48, 74, 1153, 2233, 2289, 2461
BstDEI	CTNAG	7 cut(s)	99, 186, 303, 483, 654, 1334, 2226
BstDSI	CCRYGG	1 cut(s)	2928
BstF5I	GGATG	5 cut(s)	56, 361, 388, 1530, 1825
BstKTI	GATC	6 cut(s)	835, 1292, 1504, 2335, 2345, 2449
BstMAI	GTCTC	5 cut(s)	606, 751, 1117, 2278, 2859
BstMBI	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
BstNI	CCWGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
BstNSI	RCATGY	2 cut(s)	1237, 2759
BstSCI	CCNGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
BstSFI	CTRYAG	8 cut(s)	220, 789, 819, 2268, 2369, 2603, 2637, 2937
BstV2I	GAAGAC	2 cut(s)	1470, 2847
BstX2I	RGATCY	3 cut(s)	832, 1289, 2342
BstXI	CCANNNNNNTGG	1 cut(s)	2476
BstYI	RGATCY	3 cut(s)	832, 1289, 2342
Bsu36I	CCTNAGG	1 cut(s)	654
BsuI	GTATCC	1 cut(s)	214
BsuRI	GGCC	5 cut(s)	1253, 1471, 1757, 2459, 2879
BtgI	CCRYGG	1 cut(s)	2928
BtgZI	GCGATG	3 cut(s)	1744, 2250, 2454
BtrI	CACGTC	1 cut(s)	2675
BtsCI	GGATG	5 cut(s)	56, 361, 388, 1530, 1825
BtsI	GCAGTG	4 cut(s)	864, 2668, 2674, 2946
BtsIMutI	CAGTG	6 cut(s)	864, 870, 1083, 2668, 2674, 2946
BveI	ACCTGC	2 cut(s)	520, 2362
Cac8I	GCNNGC	6 cut(s)	48, 74, 1153, 2233, 2289, 2461
CaiI	CAGNNNCTG	2 cut(s)	1196, 2504
Cfr13I	GGNCC	3 cut(s)	309, 1903, 2084
CseI	GACGC	1 cut(s)	2907
Csp6I	GTAC	8 cut(s)	266, 480, 573, 1297, 2039, 2579, 2753, 2904
CviQI	GTAC	8 cut(s)	266, 480, 573, 1297, 2039, 2579, 2753, 2904
DdeI	CTNAG	7 cut(s)	99, 186, 303, 483, 654, 1334, 2226
DpnI	GATC	6 cut(s)	834, 1291, 1503, 2334, 2344, 2448
DpnII	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
DraI	TTTAAA	1 cut(s)	2778
EaeI	YGGCCR	1 cut(s)	2457
Eam1104I	CTCTTC	4 cut(s)	384, 587, 1532, 2907
EarI	CTCTTC	4 cut(s)	384, 587, 1532, 2907
EciI	GGCGGA	3 cut(s)	990, 1078, 1727
Ecl136II	GAGCTC	3 cut(s)	1081, 1679, 2157
Eco130I	CCWWGG	3 cut(s)	576, 990, 2928
Eco147I	AGGCCT	2 cut(s)	1471, 2879
Eco24I	GRGCYC	4 cut(s)	998, 1083, 1681, 2159
Eco31I	GGTCTC	1 cut(s)	751
Eco47I	GGWCC	3 cut(s)	309, 1903, 2084
Eco53kI	GAGCTC	3 cut(s)	1081, 1679, 2157
Eco57I	CTGAAG	4 cut(s)	522, 848, 2321, 2345
Eco72I	CACGTG	2 cut(s)	2174, 2192
Eco81I	CCTNAGG	1 cut(s)	654
Eco88I	CYCGRG	1 cut(s)	2873
EcoICRI	GAGCTC	3 cut(s)	1081, 1679, 2157
EcoRII	CCWGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
EcoT14I	CCWWGG	3 cut(s)	576, 990, 2928
EcoT38I	GRGCYC	4 cut(s)	998, 1083, 1681, 2159
ErhI	CCWWGG	3 cut(s)	576, 990, 2928
FalI	AAGNNNNNCTT	2 cut(s)	727, 759
FaqI	GGGAC	2 cut(s)	262, 1880
FauI	CCCGC	1 cut(s)	43
FokI	GGATG	5 cut(s)	43, 368, 395, 1517, 1832
FriOI	GRGCYC	4 cut(s)	998, 1083, 1681, 2159
GsuI	CTGGAG	4 cut(s)	1068, 1157, 1285, 2481
HaeIII	GGCC	5 cut(s)	1253, 1471, 1757, 2459, 2879
HapII	CCGG	3 cut(s)	1027, 1534, 1874
HgaI	GACGC	1 cut(s)	2907
Hin1I	GRCGYC	2 cut(s)	1893, 2918
HincII	GTYRAC	1 cut(s)	1393
HindII	GTYRAC	1 cut(s)	1393
HpaII	CCGG	3 cut(s)	1027, 1534, 1874
HphI	GGTGA	8 cut(s)	482, 715, 870, 1705, 1901, 2147, 2386, 2435
Hpy166II	GTNNAC	6 cut(s)	559, 878, 1393, 2431, 2672, 2906
Hpy8I	GTNNAC	6 cut(s)	559, 878, 1393, 2431, 2672, 2906
HpyCH4III	ACNGT	4 cut(s)	619, 670, 1954, 2890
HpyCH4IV	ACGT	4 cut(s)	1893, 2173, 2191, 2674
HpyF3I	CTNAG	7 cut(s)	99, 186, 303, 483, 654, 1334, 2226
HpySE526I	ACGT	4 cut(s)	1893, 2173, 2191, 2674
Hsp92I	GRCGYC	2 cut(s)	1893, 2918
Kpn2I	TCCGGA	2 cut(s)	1026, 1533
Kzo9I	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
LguI	GCTCTTC	1 cut(s)	1532
LweI	GCATC	3 cut(s)	229, 346, 1177
MaeII	ACGT	4 cut(s)	1893, 2173, 2191, 2674
MalI	GATC	6 cut(s)	834, 1291, 1503, 2334, 2344, 2448
MboI	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
MfeI	CAATTG	1 cut(s)	2628
MflI	RGATCY	3 cut(s)	832, 1289, 2342
MhlI	GDGCHC	6 cut(s)	998, 1083, 1681, 1918, 2159, 2379
MlyI	GAGTC	7 cut(s)	425, 1164, 1848, 2195, 2207, 2592, 2964
MroI	TCCGGA	2 cut(s)	1026, 1533
MslI	CAYNNNNRTG	2 cut(s)	195, 1715
MspA1I	CMGCKG	1 cut(s)	626
MspCI	CTTAAG	2 cut(s)	269, 733
MspI	CCGG	3 cut(s)	1027, 1534, 1874
MspR9I	CCNGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
MunI	CAATTG	1 cut(s)	2628
Mva1269I	GAATGC	1 cut(s)	2724
MvaI	CCWGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
NcoI	CCATGG	1 cut(s)	2928
NdeII	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
NlaIV	GGNNCC	3 cut(s)	310, 1025, 2086
NmeAIII	GCCGAG	1 cut(s)	2255
NmuCI	GTSAC	6 cut(s)	163, 417, 1693, 1889, 2187, 2392
NspI	RCATGY	2 cut(s)	1237, 2759
NspV	TTCGAA	1 cut(s)	2737
PaeR7I	CTCGAG	1 cut(s)	2873
PaqCI	CACCTGC	1 cut(s)	2362
PceI	AGGCCT	2 cut(s)	1471, 2879
PciI	ACATGT	1 cut(s)	2755
PciSI	GCTCTTC	1 cut(s)	1532
PctI	GAATGC	1 cut(s)	2724
PflMI	CCANNNNNTGG	1 cut(s)	1613
PfoI	TCCNGGA	1 cut(s)	1648
PleI	GAGTC	7 cut(s)	424, 1164, 1847, 2194, 2206, 2592, 2963
PmaCI	CACGTG	2 cut(s)	2174, 2192
PmlI	CACGTG	2 cut(s)	2174, 2192
PpsI	GAGTC	7 cut(s)	424, 1164, 1847, 2194, 2206, 2592, 2963
Ppu21I	YACGTR	2 cut(s)	2174, 2192
PscI	ACATGT	1 cut(s)	2755
PshBI	ATTAAT	1 cut(s)	1269
Psp124BI	GAGCTC	3 cut(s)	1083, 1681, 2159
Psp6I	CCWGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
PspCI	CACGTG	2 cut(s)	2174, 2192
PspGI	CCWGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
PspN4I	GGNNCC	3 cut(s)	310, 1025, 2086
PspPI	GGNCC	3 cut(s)	309, 1903, 2084
PstI	CTGCAG	4 cut(s)	2272, 2373, 2641, 2941
PstNI	CAGNNNCTG	2 cut(s)	1196, 2504
PsuI	RGATCY	3 cut(s)	832, 1289, 2342
PvuII	CAGCTG	1 cut(s)	626
RsaI	GTAC	8 cut(s)	267, 481, 574, 1298, 2040, 2580, 2754, 2905
RsaNI	GTAC	8 cut(s)	266, 480, 573, 1297, 2039, 2579, 2753, 2904
RseI	CAYNNNNRTG	2 cut(s)	195, 1715
SacI	GAGCTC	3 cut(s)	1083, 1681, 2159
SapI	GCTCTTC	1 cut(s)	1532
Sau3AI	GATC	6 cut(s)	832, 1289, 1501, 2332, 2342, 2446
Sau96I	GGNCC	3 cut(s)	309, 1903, 2084
SbfI	CCTGCAGG	1 cut(s)	2373
ScaI	AGTACT	1 cut(s)	481
SchI	GAGTC	7 cut(s)	425, 1164, 1848, 2195, 2207, 2592, 2964
ScrFI	CCNGG	7 cut(s)	939, 1005, 1047, 1175, 1303, 1650, 2499
SdaI	CCTGCAGG	1 cut(s)	2373
SduI	GDGCHC	6 cut(s)	998, 1083, 1681, 1918, 2159, 2379
SfaNI	GCATC	3 cut(s)	229, 346, 1177
SfcI	CTRYAG	8 cut(s)	220, 789, 819, 2268, 2369, 2603, 2637, 2937
Sfr274I	CTCGAG	1 cut(s)	2873
SfuI	TTCGAA	1 cut(s)	2737
SinI	GGWCC	3 cut(s)	309, 1903, 2084
SlaI	CTCGAG	1 cut(s)	2873
SmiMI	CAYNNNNRTG	2 cut(s)	195, 1715
SmlI	CTYRAG	5 cut(s)	269, 733, 960, 2382, 2873
SmoI	CTYRAG	5 cut(s)	269, 733, 960, 2382, 2873
SpeI	ACTAGT	1 cut(s)	368
Sse8387I	CCTGCAGG	1 cut(s)	2373
SseBI	AGGCCT	2 cut(s)	1471, 2879
SsiI	CCGC	8 cut(s)	50, 323, 1001, 1063, 1163, 1738, 2456, 2721
SstI	GAGCTC	3 cut(s)	1083, 1681, 2159
StuI	AGGCCT	2 cut(s)	1471, 2879
StyD4I	CCNGG	7 cut(s)	937, 1003, 1045, 1173, 1301, 1648, 2497
StyI	CCWWGG	3 cut(s)	576, 990, 2928
TaaI	ACNGT	4 cut(s)	619, 670, 1954, 2890
TaiI	ACGT	4 cut(s)	1896, 2176, 2194, 2677
TaqI	TCGA	8 cut(s)	476, 563, 945, 1437, 2349, 2737, 2874, 2958
TatI	WGTACW	4 cut(s)	265, 479, 1296, 2752
TauI	GCSGC	3 cut(s)	1166, 2459, 2724
TscAI	CASTG	6 cut(s)	864, 877, 1090, 2668, 2674, 2946
TseFI	GTSAC	6 cut(s)	163, 417, 1693, 1889, 2187, 2392
Tsp45I	GTSAC	6 cut(s)	163, 417, 1693, 1889, 2187, 2392
TspDTI	ATGAA	7 cut(s)	45, 1287, 1455, 2091, 2154, 2226, 2712
TspGWI	ACGGA	1 cut(s)	151
TspRI	CASTG	6 cut(s)	864, 877, 1090, 2668, 2674, 2946
Van91I	CCANNNNNTGG	1 cut(s)	1613
Vha464I	CTTAAG	2 cut(s)	269, 733
VpaK11BI	GGWCC	3 cut(s)	309, 1903, 2084
VspI	ATTAAT	1 cut(s)	1269
XapI	RAATTY	4 cut(s)	155, 920, 1281, 2141
XbaI	TCTAGA	1 cut(s)	1213
XceI	RCATGY	2 cut(s)	1237, 2759
XhoI	CTCGAG	1 cut(s)	2873
ZraI	GACGTC	1 cut(s)	1894
ZrmI	AGTACT	1 cut(s)	481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.