Rh5CG208200

Translation initiation factor IF-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011012
Physical Location & Seq
Forward (+)
20985088 .. 20991799
6712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG208200.1

Sequence Viewer

Length: 2835 bp
ATGTTCATATTAGTTGGAAGCATGCAGGGAACAATGGTTTCTTTGGCTTCCCTTGTTAGTTTGGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTGTTAGGAAGGTTTCCTTGTCTAAGAGTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGATTGTCAGTTTGTAAATTTTCGGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTATCCCTTGATTCTAACTATAGAGGAAGTAGTAATGATGCTTCTGTTGCCAATGCTGATTTTGTACTTAAGCCGTCCCCGAAGCCAGTATTGAAGCCCTCTGAGGGGTCCAATGCTGAACCGCCCCTTTTAAGCCTTAATGCTGCTGATTGGGACGCCTCAAAAACTAGTGGGGATTCGGATGTGGAAGAGGTAGATAAGAGTAATGTGATTGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTAGAAGCTCCAAAAGTGGATGATTCGAGTACTAAGAAAGCCAGTAAGCCTGTAAATAGACCTGCACCTTCTAATGCAACTACTACTTCAGGAAATGCTAGACCAGTAAACTCGACAGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGAAAAGGAGACACAGTTGCAGCTGTGCAAAAGGTTGTAAAGGAATTACCTAAGGTTAATAATACAGTTCGGAGAGAAGAACCAAAAACAGGGGGAGGGGTGAAGGTAGAGTCTACACCCGGTCCTCCTTTAAGACCTCCTCCTCCACCTTTGAGACCCCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTCTGAAGGATCTTGGGGCAGCTCCAAAGTCGGCCGTGATTGATGACACTGGTTCACCCCCAAAAACTAAAGAAAGGAAGCCAATTTTGATTGACAAATTTTCTACCAAGAAGCCAGGGGTCGATTCTGTGGTTTCTCAAGCAGTTTTAGCTCCTACAAAACCAGCCAAGGGCTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCTGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTCACTGATGATGAGAGTTCAGAACTCAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCAAGACTCCAGGCTGCCAAAGATGCAGCTCCTGTTAAAGTAGATATTCTAGAGGTTGATGACGATGGCATGTTAATAGATGAGTTGGCCTTCAATTTGGCTATTAATGAATCCGAAATTCTCGGATCTCTGTACTCCAGGGGAATTAAGCCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTGAAGATGATTTGTAAAGAGTATGATGTGGAAGTCATAGATGTTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTCGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTTACTATAATGGGTCATGTGGATCATGGCAAGACAACTCTATTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACACAAGGAATTGGGGCATACAAGGTGCTTGTGCCAGTTGATGGCAAGTTGCAATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGAGCAATGAGAGCTCGTGGAGCAAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCGATAGTAATAGCTATAAATAAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTTTTGGAAACCGTAATGCTAGTTGCAGAGGTACGGGCTTTATTTGATGATGGTGGAAATCGTGTCAATGAAGCTGGACCCACTATACCAGTTCAGGTTATTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAGGTCGTGAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTCACGTGCAGAGTCATTGCGAAATGAACGCATATCAGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCTTTAGCTTCTGCAGTCTCGGCAGGAAAGCTGGCTGGACTTGACTTGCACCAGCTAAATGTTATTCTGAAGGTTGATCTTCAGGGATCTATTGAGGCTGTCAGACAAGCCCTGCAGGTGCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACACCAGCGATGTTGATCTTGCTGCGGCCAGCAAAGCCATAATTTTGGGATTCAATATCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAGGGTGTGGAGATTCGGCTATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGACGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTTAAAGGCTGTGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTTCATGTTGGTGTTCTTGATTCTTTAAAACGGGTTAAGGAAGTCGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTACGACGATTTTGAGGAGGGAGACATTCTCGAGGCCTTCAACACAGTCCAAAAGAAGCGGACACTTGAAGAGGCGTCTGCTTCCATGGCAGCTGCAGTGGAAGGAACAGGAGTCGAATACTAG

Protein Analysis

944

Amino Acids

100.51

Weight (kDa)

8.14

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 505 - 631 3.2e-26 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 506 - 614 1.8e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 633 - 673 3e-08 Elongation factor G domain 2
IF-2 PF11987 723 - 817 1.5e-31 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2230
Acc36I ACCTGC 2 cut(s) 553, 2230
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccI GTMKAC 1 cut(s) 749
AccIII TCCGGA 2 cut(s) 1059, 1566
AciI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2324, 2770
AclWI GGATC 4 cut(s) 873, 1330, 1542, 2218
AcoI YGGCCR 2 cut(s) 888, 2325
AcsI RAATTY 4 cut(s) 188, 953, 1314, 2009
AcuI CTGAAG 4 cut(s) 555, 881, 2189, 2213
AcvI CACGTG 2 cut(s) 2042, 2060
AcyI GRCGYC 2 cut(s) 390, 2786
AfaI GTAC 6 cut(s) 300, 514, 607, 1331, 1908, 2448
AfiI CCNNNNNNNGG 7 cut(s) 338, 339, 725, 1025, 1435, 1646, 2000
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 2542
AgsI TTSAA 9 cut(s) 328, 625, 1048, 1291, 1361, 2269, 2353, 2752, 2780
AhlI ACTAGT 1 cut(s) 401
AjiI CACGTC 1 cut(s) 2543
AjnI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2365
AloI GAACNNNNNNTCC 2 cut(s) 2604, 2636
Alw21I GWGCWC 5 cut(s) 1116, 1714, 1852, 2027, 2247
Alw26I GTCTC 5 cut(s) 639, 784, 1150, 2146, 2727
AlwI GGATC 4 cut(s) 873, 1330, 1542, 2218
AlwNI CAGNNNCTG 2 cut(s) 1229, 2372
Ama87I CYCGRG 1 cut(s) 2741
Aor13HI TCCGGA 2 cut(s) 1059, 1566
AoxI GGCC 6 cut(s) 888, 1284, 1502, 1788, 2325, 2745
ApoI RAATTY 4 cut(s) 188, 953, 1314, 2009
AseI ATTAAT 1 cut(s) 1302
AspS9I GGNCC 3 cut(s) 342, 758, 1952
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 7 cut(s) 748, 903, 1393, 1738, 2015, 2254, 2303
AsuII TTCGAA 1 cut(s) 2605
AvaI CYCGRG 1 cut(s) 2741
AvaII GGWCC 3 cut(s) 342, 758, 1952
AxyI CCTNAGG 1 cut(s) 687
BanII GRGCYC 4 cut(s) 1031, 1116, 1714, 2027
BarI GAAGNNNNNNTAC 6 cut(s) 58, 90, 259, 291, 2454, 2486
BauI CACGAG 1 cut(s) 1713
BbrPI CACGTG 2 cut(s) 2042, 2060
BbsI GAAGAC 2 cut(s) 1503, 2715
Bbv12I GWGCWC 5 cut(s) 1116, 1714, 1852, 2027, 2247
BccI CCATC 9 cut(s) 153, 1046, 1256, 1346, 1640, 1757, 1799, 1919, 2099
BceAI ACGGC 2 cut(s) 292, 875
BciT130I CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2367
BciVI GTATCC 1 cut(s) 247
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 5 cut(s) 639, 784, 1150, 2146, 2727
BcuI ACTAGT 1 cut(s) 401
BfmI CTRYAG 8 cut(s) 253, 822, 852, 2136, 2237, 2471, 2505, 2805
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 2 cut(s) 553, 2230
BfuI GTATCC 1 cut(s) 247
BglI GCCNNNNNGGC 1 cut(s) 1592
BlpI GCTNAGC 1 cut(s) 219
BmcAI AGTACT 1 cut(s) 514
Bme1390I CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2367
Bme18I GGWCC 3 cut(s) 342, 758, 1952
BmeT110I CYCGRG 1 cut(s) 2741
BmgBI CACGTC 1 cut(s) 2543
BmgT120I GGNCC 3 cut(s) 342, 758, 1952
BmiI GGNNCC 3 cut(s) 343, 1058, 1954
BmrFI CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2367
BmrI ACTGGG 1 cut(s) 1424
BmsI GCATC 2 cut(s) 262, 1210
BmuI ACTGGG 1 cut(s) 1424
BpiI GAAGAC 2 cut(s) 1503, 2715
BplI GAGNNNNNCTC 2 cut(s) 2724, 2756
BpmI CTGGAG 4 cut(s) 1101, 1190, 1318, 2349
Bpu10I CCTNAGC 1 cut(s) 2094
Bpu1102I GCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 2605
BpuEI CTTGAG 2 cut(s) 978, 2235
BpuMI CCSGG 1 cut(s) 756
BsaAI YACGTR 2 cut(s) 2042, 2060
BsaBI GATNNNNATC 1 cut(s) 2313
BsaHI GRCGYC 2 cut(s) 390, 2786
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 6 cut(s) 609, 971, 1023, 1335, 1590, 2796
BsaWI WCCGGW 2 cut(s) 1059, 1566
BsaXI ACNNNNNCTCC 6 cut(s) 636, 666, 814, 844, 1710, 1740
Bsc4I CCNNNNNNNGG 7 cut(s) 338, 339, 725, 1025, 1435, 1646, 2000
Bse1I ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 1964
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 4 cut(s) 235, 1710, 2069, 2463
Bse8I GATNNNNATC 1 cut(s) 2313
BseAI TCCGGA 2 cut(s) 1059, 1566
BseBI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2367
BseDI CCNNGG 6 cut(s) 609, 971, 1023, 1335, 1590, 2796
BseGI GGATG 4 cut(s) 89, 421, 508, 1563
BseJI GATNNNNATC 1 cut(s) 2313
BseLI CCNNNNNNNGG 7 cut(s) 338, 339, 725, 1025, 1435, 1646, 2000
BseMI GCAATG 4 cut(s) 235, 1710, 2069, 2463
BseMII CTCAG 3 cut(s) 210, 327, 1358
BseNI ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 1964
BseRI GAGGAG 4 cut(s) 765, 768, 819, 2741
BseX3I CGGCCG 1 cut(s) 888
BsgI GTGCAG 2 cut(s) 531, 2082
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 6 cut(s) 890, 1286, 1504, 1790, 2327, 2747
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 5 cut(s) 1116, 1714, 1852, 2027, 2247
BsiHKCI CYCGRG 1 cut(s) 2741
BsiSI CCGG 3 cut(s) 756, 1060, 1567
BslFI GGGAC 2 cut(s) 295, 401
BslI CCNNNNNNNGG 7 cut(s) 338, 339, 725, 1025, 1435, 1646, 2000
BsmAI GTCTC 5 cut(s) 639, 784, 1150, 2146, 2727
BsmFI GGGAC 2 cut(s) 295, 401
BsmI GAATGC 1 cut(s) 2592
BsnI GGCC 6 cut(s) 890, 1286, 1504, 1790, 2327, 2747
Bso31I GGTCTC 1 cut(s) 784
BsoBI CYCGRG 1 cut(s) 2741
Bsp119I TTCGAA 1 cut(s) 2605
Bsp1286I GDGCHC 6 cut(s) 1031, 1116, 1714, 1852, 2027, 2247
Bsp13I TCCGGA 2 cut(s) 1059, 1566
Bsp143I GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 1 cut(s) 2796
BspACI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2324, 2770
BspANI GGCC 6 cut(s) 890, 1286, 1504, 1790, 2327, 2747
BspCNI CTCAG 3 cut(s) 211, 328, 1359
BspEI TCCGGA 2 cut(s) 1059, 1566
BspLI GGNNCC 3 cut(s) 343, 1058, 1954
BspMAI CTGCAG 4 cut(s) 2140, 2241, 2509, 2809
BspMI ACCTGC 2 cut(s) 553, 2230
BspPI GGATC 4 cut(s) 873, 1330, 1542, 2218
BspQI GCTCTTC 1 cut(s) 1565
BspT104I TTCGAA 1 cut(s) 2605
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 784
BsrDI GCAATG 4 cut(s) 235, 1710, 2069, 2463
BsrI ACTGG 7 cut(s) 320, 525, 587, 910, 1430, 1640, 1964
BssECI CCNNGG 6 cut(s) 609, 971, 1023, 1335, 1590, 2796
BssMI GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
BssNI GRCGYC 2 cut(s) 390, 2786
BssSI CACGAG 1 cut(s) 1713
BssT1I CCWWGG 3 cut(s) 609, 1023, 2796
Bst2BI CACGAG 1 cut(s) 1713
Bst2UI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2367
Bst4CI ACNGT 4 cut(s) 652, 703, 1888, 2758
Bst6I CTCTTC 4 cut(s) 417, 620, 1565, 2775
BstACI GRCGYC 2 cut(s) 390, 2786
BstAFI CTTAAG 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 2042, 2060
BstBI TTCGAA 1 cut(s) 2605
BstC8I GCNNGC 7 cut(s) 23, 81, 107, 1186, 2101, 2157, 2329
BstDEI CTNAG 7 cut(s) 132, 219, 336, 516, 687, 1367, 2094
BstDSI CCRYGG 1 cut(s) 2796
BstF5I GGATG 4 cut(s) 89, 421, 508, 1563
BstKTI GATC 6 cut(s) 868, 1325, 1537, 2203, 2213, 2317
BstMAI GTCTC 5 cut(s) 639, 784, 1150, 2146, 2727
BstMBI GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
BstMCI CGRYCG 1 cut(s) 891
BstNI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2367
BstNSI RCATGY 2 cut(s) 25, 1270
BstSCI CCNGG 8 cut(s) 754, 970, 1036, 1078, 1206, 1334, 1681, 2365
BstSFI CTRYAG 8 cut(s) 253, 822, 852, 2136, 2237, 2471, 2505, 2805
BstV2I GAAGAC 2 cut(s) 1503, 2715
BstX2I RGATCY 3 cut(s) 865, 1322, 2210
BstXI CCANNNNNNTGG 1 cut(s) 2344
BstYI RGATCY 3 cut(s) 865, 1322, 2210
BstZI CGGCCG 1 cut(s) 888
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 6 cut(s) 890, 1286, 1504, 1790, 2327, 2747
BtgI CCRYGG 1 cut(s) 2796
BtgZI GCGATG 3 cut(s) 1777, 2118, 2322
BtrI CACGTC 1 cut(s) 2543
BtsCI GGATG 4 cut(s) 89, 421, 508, 1563
BtsI GCAGTG 3 cut(s) 2536, 2542, 2814
BtsIMutI CAGTG 5 cut(s) 903, 1116, 2536, 2542, 2814
BveI ACCTGC 2 cut(s) 553, 2230
Cac8I GCNNGC 7 cut(s) 23, 81, 107, 1186, 2101, 2157, 2329
CaiI CAGNNNCTG 2 cut(s) 1229, 2372
Cfr13I GGNCC 3 cut(s) 342, 758, 1952
CseI GACGC 2 cut(s) 398, 2775
Csp6I GTAC 6 cut(s) 299, 513, 606, 1330, 1907, 2447
CviQI GTAC 6 cut(s) 299, 513, 606, 1330, 1907, 2447
DdeI CTNAG 7 cut(s) 132, 219, 336, 516, 687, 1367, 2094
DpnI GATC 6 cut(s) 867, 1324, 1536, 2202, 2212, 2316
DpnII GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
DraI TTTAAA 1 cut(s) 2646
EaeI YGGCCR 2 cut(s) 888, 2325
EagI CGGCCG 1 cut(s) 888
Eam1104I CTCTTC 4 cut(s) 417, 620, 1565, 2775
EarI CTCTTC 4 cut(s) 417, 620, 1565, 2775
EciI GGCGGA 3 cut(s) 1023, 1111, 1760
Ecl136II GAGCTC 3 cut(s) 1114, 1712, 2025
EclXI CGGCCG 1 cut(s) 888
Eco130I CCWWGG 3 cut(s) 609, 1023, 2796
Eco147I AGGCCT 2 cut(s) 1504, 2747
Eco24I GRGCYC 4 cut(s) 1031, 1116, 1714, 2027
Eco31I GGTCTC 1 cut(s) 784
Eco47I GGWCC 3 cut(s) 342, 758, 1952
Eco52I CGGCCG 1 cut(s) 888
Eco53kI GAGCTC 3 cut(s) 1114, 1712, 2025
Eco57I CTGAAG 4 cut(s) 555, 881, 2189, 2213
Eco72I CACGTG 2 cut(s) 2042, 2060
Eco81I CCTNAGG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 2741
EcoICRI GAGCTC 3 cut(s) 1114, 1712, 2025
EcoRII CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2365
EcoT14I CCWWGG 3 cut(s) 609, 1023, 2796
EcoT38I GRGCYC 4 cut(s) 1031, 1116, 1714, 2027
ErhI CCWWGG 3 cut(s) 609, 1023, 2796
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 2 cut(s) 295, 401
FauI CCCGC 1 cut(s) 76
FblI GTMKAC 1 cut(s) 749
FokI GGATG 4 cut(s) 76, 428, 515, 1550
FriOI GRGCYC 4 cut(s) 1031, 1116, 1714, 2027
GsuI CTGGAG 4 cut(s) 1101, 1190, 1318, 2349
HaeIII GGCC 6 cut(s) 890, 1286, 1504, 1790, 2327, 2747
HapII CCGG 3 cut(s) 756, 1060, 1567
HgaI GACGC 2 cut(s) 398, 2775
Hin1I GRCGYC 2 cut(s) 390, 2786
HincII GTYRAC 1 cut(s) 1426
HindII GTYRAC 1 cut(s) 1426
HpaII CCGG 3 cut(s) 756, 1060, 1567
HphI GGTGA 7 cut(s) 748, 903, 1393, 1738, 2015, 2254, 2303
Hpy166II GTNNAC 6 cut(s) 592, 750, 911, 1426, 2299, 2540
Hpy188III TCNNGA 9 cut(s) 573, 1060, 1247, 1567, 2020, 2252, 2636, 2665, 2741
Hpy8I GTNNAC 6 cut(s) 592, 750, 911, 1426, 2299, 2540
Hpy99I CGWCG 1 cut(s) 2720
HpyCH4III ACNGT 4 cut(s) 652, 703, 1888, 2758
HpyCH4IV ACGT 3 cut(s) 2041, 2059, 2542
HpyF3I CTNAG 7 cut(s) 132, 219, 336, 516, 687, 1367, 2094
HpySE526I ACGT 3 cut(s) 2041, 2059, 2542
Hsp92I GRCGYC 2 cut(s) 390, 2786
Kpn2I TCCGGA 2 cut(s) 1059, 1566
Kzo9I GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
LguI GCTCTTC 1 cut(s) 1565
LweI GCATC 2 cut(s) 262, 1210
MaeII ACGT 3 cut(s) 2041, 2059, 2542
MaeIII GTNAC 8 cut(s) 73, 196, 450, 1726, 2055, 2113, 2260, 2491
MalI GATC 6 cut(s) 867, 1324, 1536, 2202, 2212, 2316
MboI GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
MfeI CAATTG 1 cut(s) 2496
MflI RGATCY 3 cut(s) 865, 1322, 2210
MhlI GDGCHC 6 cut(s) 1031, 1116, 1714, 1852, 2027, 2247
MlyI GAGTC 7 cut(s) 458, 755, 1197, 2063, 2075, 2460, 2832
MroI TCCGGA 2 cut(s) 1059, 1566
MslI CAYNNNNRTG 3 cut(s) 228, 1748, 2628
MspA1I CMGCKG 2 cut(s) 659, 2804
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 3 cut(s) 756, 1060, 1567
MspR9I CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2367
MunI CAATTG 1 cut(s) 2496
Mva1269I GAATGC 1 cut(s) 2592
MvaI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2367
NciI CCSGG 1 cut(s) 756
NcoI CCATGG 1 cut(s) 2796
NdeII GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
NlaIV GGNNCC 3 cut(s) 343, 1058, 1954
NmeAIII GCCGAG 1 cut(s) 2123
NmuCI GTSAC 5 cut(s) 196, 450, 1726, 2055, 2260
NspI RCATGY 2 cut(s) 25, 1270
NspV TTCGAA 1 cut(s) 2605
PaeI GCATGC 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 2741
PaqCI CACCTGC 1 cut(s) 2230
PceI AGGCCT 2 cut(s) 1504, 2747
PciSI GCTCTTC 1 cut(s) 1565
PctI GAATGC 1 cut(s) 2592
PflMI CCANNNNNTGG 1 cut(s) 1646
PfoI TCCNGGA 1 cut(s) 1681
PleI GAGTC 7 cut(s) 457, 754, 1197, 2062, 2074, 2460, 2831
PmaCI CACGTG 2 cut(s) 2042, 2060
PmlI CACGTG 2 cut(s) 2042, 2060
PpsI GAGTC 7 cut(s) 457, 754, 1197, 2062, 2074, 2460, 2831
Ppu21I YACGTR 2 cut(s) 2042, 2060
PshBI ATTAAT 1 cut(s) 1302
Psp124BI GAGCTC 3 cut(s) 1116, 1714, 2027
Psp6I CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2365
PspCI CACGTG 2 cut(s) 2042, 2060
PspGI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2365
PspN4I GGNNCC 3 cut(s) 343, 1058, 1954
PspPI GGNCC 3 cut(s) 342, 758, 1952
PstI CTGCAG 4 cut(s) 2140, 2241, 2509, 2809
PstNI CAGNNNCTG 2 cut(s) 1229, 2372
PsuI RGATCY 3 cut(s) 865, 1322, 2210
PvuII CAGCTG 2 cut(s) 659, 2804
RsaI GTAC 6 cut(s) 300, 514, 607, 1331, 1908, 2448
RsaNI GTAC 6 cut(s) 299, 513, 606, 1330, 1907, 2447
RseI CAYNNNNRTG 3 cut(s) 228, 1748, 2628
SacI GAGCTC 3 cut(s) 1116, 1714, 2027
SapI GCTCTTC 1 cut(s) 1565
Sau3AI GATC 6 cut(s) 865, 1322, 1534, 2200, 2210, 2314
Sau96I GGNCC 3 cut(s) 342, 758, 1952
SbfI CCTGCAGG 1 cut(s) 2241
ScaI AGTACT 1 cut(s) 514
SchI GAGTC 7 cut(s) 458, 755, 1197, 2063, 2075, 2460, 2832
ScrFI CCNGG 8 cut(s) 756, 972, 1038, 1080, 1208, 1336, 1683, 2367
SdaI CCTGCAGG 1 cut(s) 2241
SduI GDGCHC 6 cut(s) 1031, 1116, 1714, 1852, 2027, 2247
SfaNI GCATC 2 cut(s) 262, 1210
SfcI CTRYAG 8 cut(s) 253, 822, 852, 2136, 2237, 2471, 2505, 2805
Sfr274I CTCGAG 1 cut(s) 2741
SfuI TTCGAA 1 cut(s) 2605
SinI GGWCC 3 cut(s) 342, 758, 1952
SlaI CTCGAG 1 cut(s) 2741
SmiMI CAYNNNNRTG 3 cut(s) 228, 1748, 2628
SmlI CTYRAG 4 cut(s) 302, 993, 2250, 2741
SmoI CTYRAG 4 cut(s) 302, 993, 2250, 2741
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 25
Sse8387I CCTGCAGG 1 cut(s) 2241
SseBI AGGCCT 2 cut(s) 1504, 2747
SsiI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2324, 2770
SstI GAGCTC 3 cut(s) 1116, 1714, 2027
StuI AGGCCT 2 cut(s) 1504, 2747
StyD4I CCNGG 8 cut(s) 754, 970, 1036, 1078, 1206, 1334, 1681, 2365
StyI CCWWGG 3 cut(s) 609, 1023, 2796
TaaI ACNGT 4 cut(s) 652, 703, 1888, 2758
TaiI ACGT 3 cut(s) 2044, 2062, 2545
TaqI TCGA 7 cut(s) 509, 596, 978, 1470, 2605, 2742, 2826
TaqII GACCGA 1 cut(s) 184
TatI WGTACW 3 cut(s) 298, 512, 1329
TauI GCSGC 2 cut(s) 1199, 2327
TscAI CASTG 5 cut(s) 910, 1123, 2536, 2542, 2814
TseFI GTSAC 5 cut(s) 196, 450, 1726, 2055, 2260
Tsp45I GTSAC 5 cut(s) 196, 450, 1726, 2055, 2260
TspDTI ATGAA 8 cut(s) 78, 1320, 1488, 1959, 2022, 2094, 2580, 2612
TspRI CASTG 5 cut(s) 910, 1123, 2536, 2542, 2814
Van91I CCANNNNNTGG 1 cut(s) 1646
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 342, 758, 1952
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 4 cut(s) 188, 953, 1314, 2009
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 2 cut(s) 25, 1270
XhoI CTCGAG 1 cut(s) 2741
XmiI GTMKAC 1 cut(s) 749
ZrmI AGTACT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.