Rh5AG190800

Translation initiation factor IF-2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011012
Physical Location & Seq
Forward (+)
21975075 .. 21985209
10135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG190800.1

Sequence Viewer

Length: 2961 bp
ATGCTCATATTAGTTGGAAGCATGCAGGGAACAATGGTCTCTTTGGCTTCCCTTGTTAGTTTGGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTGTTAGGAAGGTTTCGTTGTCTAAGAGTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGATTGTCAGTTTGTAAATTTTCGGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTATCCCTTGATTCTAACTATAGAGGAAGTAGTAATGATGCTTCTGTTGCCAATGCTGATTTTGTACTTAAGCCGTCCCCGAAGCCAGTATTGAAGCCCTCTGAGGGGTCCAATGCTGAACCGCCCCTTTTAAGCCTTAATGCTGCTGATTGGGACGCCTCAAAAACTAGTGGGGATTCGGTTGTGGATGAGGAAGATAAGAGTAATGTGATCGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTAGAAGCTCCAAAAGTGGGTGATTCGAATACTAAGAAAGCCAGTAAGCCTGTAAATAGACCTGCACCTTCTAATGCAACTACTACTTCAGGAAATGCTAGACAAGTAAACTCGACAGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGTAAAGGAGACACAGTTGCAGCTGTGCAAAAGGTTGTAAAGGAATTACCTAAGGTTAATAATACAGTTCGGAGAGAAGAACCAAAAACAGGGGAAGGGGTGAAGGTAGAGTCGCCAACCCGTCCTCCTTTAAGACCTCCTTCTCCACCTTTGAGACCCCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTCTGAAGGATCTTGGGGCAGCTCCAAAGTCGGCAGTGATTGATGACGCTGGTTCACCCCCAAAAACTAAAGAAAGAAAGCCAATTTTAATTGACAAATTTTCTACCAAGAAGCCAGGGGTCGATTCTGTGGTTTCTCAAGCAGTTTTAGCTCCTACAAAACCAGCCAAGGGGTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCTGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTCACTGATGATGAGAGTTCGGAACTCAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCAAGACTCCAGGCTGCCAAAGATGCAGCTCCTGTTAAAGTAGATATTCTAGAGGTTGATGACGATGGCATGTTAATAGATGAGTTGGCCTTCAATTTGGCTATTAATGAATCCGAAATTCTCGGATCTCTGTACTCCAGGGGAATTAAGCCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTAAAGATGATTTGTAAAGAGTATGATGTGGAAGTCATAGATGTTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTTGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTTACTATAATGGGTCATGTGGATCATGGCAAGACAACTCTATTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACACAAGGAATTGGGGCATACAAGGTGCTTGTGCCAGTTGATGGCAAGTTGCAATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGAGCAATGAGAGCTCGTGGAGCAAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCAATAGTAATAGCTATAAATAAGATAGACAAGGATGGAGCTAATCCAGAACGAGTCATGCAAGAGCTTTCTTCTATTGGTCTAATGCCGGAGGATTGGGGTGGTGACGTCCCAATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTGTTGGAAACCGTAATGCTAGTTGCAGAGTTGCAAGAGTTAAAGGCCAATCCTGATAGAAGTGCAAAAGGCACAGTTATTGAGGCCGGTCTTGATAAATCCAGAGGACCTTTAGTTACACTAATCGTGCAAAATGGAACCCTTAGAAAGGGGGACATAGTAGTTTGTGGAGAAGCCTTTGGAAAGGTACGGGCTTTATTTGATGATGGTGGAAATCGTGTCAATGAAGCTGGACCCTCTATACCAGTTCAGGTTATTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAGGTCGTGAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTCACGTGCAGAGTCATTGCGAAATGAACGCATATCAGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCTTTAGCTTCTGCAGTCTCGGCAGGAAAGCTGGCTGGACTTGACTTGCACCAGCTAAATGTTATTCTGAAGGTTGATCTTCAGGGATCTATCGAGGCTGTCAGACAAGCCCTGCAGGTGCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACACCAGCGATGTTGATCTTGCTGCGGCCAGCAAAGCCATAATTTTGGGATTCAATATCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAAGGTGTGGAGATTCGGCTATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGACGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTTAAAGGCTGCGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTACATGTTGGTGTTCTTGATTCTTTAAAACGGGTTAAGGAAGTCGTGAAAGAGGTATTATATCATAGCCTGCATTTACTTATATGA

Protein Analysis

986

Amino Acids

105.2

Weight (kDa)

8.89

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 505 - 665 3.6e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 506 - 614 1.9e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 680 - 758 1.3e-26 Elongation factor G domain 2
IF-2 PF11987 808 - 902 1.6e-31 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2485
AatII GACGTC 1 cut(s) 1929
Acc36I ACCTGC 2 cut(s) 553, 2485
AccB7I CCANNNNNTGG 1 cut(s) 1646
AccIII TCCGGA 2 cut(s) 1059, 1566
AciI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 2844
AclWI GGATC 4 cut(s) 873, 1330, 1542, 2473
AcoI YGGCCR 1 cut(s) 2580
AcsI RAATTY 4 cut(s) 188, 953, 1314, 2264
AcuI CTGAAG 4 cut(s) 555, 881, 2444, 2468
AcvI CACGTG 2 cut(s) 2297, 2315
AcyI GRCGYC 2 cut(s) 390, 1926
AfaI GTAC 6 cut(s) 300, 607, 1331, 2163, 2703, 2877
AfiI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 725, 1025, 1435, 1646, 2122, 2255
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 2 cut(s) 2797, 2878
AgsI TTSAA 7 cut(s) 328, 625, 1048, 1291, 1361, 2524, 2608
AhlI ACTAGT 1 cut(s) 401
AjiI CACGTC 1 cut(s) 2798
AjnI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
Alw21I GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
Alw26I GTCTC 6 cut(s) 43, 639, 784, 1035, 1150, 2401
AlwI GGATC 4 cut(s) 873, 1330, 1542, 2473
AlwNI CAGNNNCTG 2 cut(s) 1229, 2627
Aor13HI TCCGGA 2 cut(s) 1059, 1566
AoxI GGCC 6 cut(s) 1284, 1502, 1788, 2019, 2058, 2580
ApoI RAATTY 4 cut(s) 188, 953, 1314, 2264
AseI ATTAAT 1 cut(s) 1302
AspS9I GGNCC 4 cut(s) 342, 1936, 2081, 2207
AsuHPI GGTGA 8 cut(s) 515, 748, 903, 1738, 1934, 2270, 2509, 2558
AsuII TTCGAA 2 cut(s) 509, 2860
AvaII GGWCC 4 cut(s) 342, 1936, 2081, 2207
AxyI CCTNAGG 1 cut(s) 687
BanII GRGCYC 3 cut(s) 1116, 1714, 2282
BarI GAAGNNNNNNTAC 6 cut(s) 58, 90, 259, 291, 2709, 2741
BauI CACGAG 1 cut(s) 1713
BbrPI CACGTG 2 cut(s) 2297, 2315
BbsI GAAGAC 1 cut(s) 1503
Bbv12I GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BciVI GTATCC 1 cut(s) 247
BcoDI GTCTC 6 cut(s) 43, 639, 784, 1035, 1150, 2401
BcuI ACTAGT 1 cut(s) 401
BfaI CTAG 8 cut(s) 402, 485, 582, 603, 1247, 1484, 1493, 1994
BfmI CTRYAG 7 cut(s) 253, 822, 852, 2391, 2492, 2726, 2760
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 2 cut(s) 553, 2485
BfuI GTATCC 1 cut(s) 247
BglI GCCNNNNNGGC 1 cut(s) 1592
BlpI GCTNAGC 1 cut(s) 219
Bme1390I CCNGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
Bme18I GGWCC 4 cut(s) 342, 1936, 2081, 2207
BmgBI CACGTC 1 cut(s) 2798
BmgT120I GGNCC 4 cut(s) 342, 1936, 2081, 2207
BmiI GGNNCC 4 cut(s) 343, 1058, 2113, 2209
BmrFI CCNGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BmrI ACTGGG 1 cut(s) 1424
BmsI GCATC 2 cut(s) 262, 1210
BmuI ACTGGG 1 cut(s) 1424
BpiI GAAGAC 1 cut(s) 1503
BpmI CTGGAG 4 cut(s) 1101, 1190, 1318, 2604
Bpu10I CCTNAGC 1 cut(s) 2349
Bpu1102I GCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 2 cut(s) 509, 2860
BpuEI CTTGAG 2 cut(s) 978, 2490
BsaAI YACGTR 2 cut(s) 2297, 2315
BsaBI GATNNNNATC 1 cut(s) 2568
BsaHI GRCGYC 2 cut(s) 390, 1926
BsaI GGTCTC 3 cut(s) 43, 784, 1035
BsaJI CCNNGG 5 cut(s) 609, 971, 1023, 1335, 1590
BsaWI WCCGGW 2 cut(s) 1059, 1566
BsaXI ACNNNNNCTCC 8 cut(s) 636, 666, 745, 775, 814, 844, 1710, 1740
Bsc4I CCNNNNNNNGG 9 cut(s) 338, 339, 500, 725, 1025, 1435, 1646, 2122, 2255
Bse118I RCCGGY 1 cut(s) 2060
Bse1I ACTGG 5 cut(s) 320, 525, 1430, 1640, 2219
Bse21I CCTNAGG 1 cut(s) 687
Bse3DI GCAATG 4 cut(s) 235, 1710, 2324, 2718
Bse8I GATNNNNATC 1 cut(s) 2568
BseAI TCCGGA 2 cut(s) 1059, 1566
BseBI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BseDI CCNNGG 5 cut(s) 609, 971, 1023, 1335, 1590
BseGI GGATG 4 cut(s) 89, 427, 1563, 1858
BseJI GATNNNNATC 1 cut(s) 2568
BseLI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 725, 1025, 1435, 1646, 2122, 2255
BseMI GCAATG 4 cut(s) 235, 1710, 2324, 2718
BseMII CTCAG 3 cut(s) 210, 327, 1358
BseNI ACTGG 5 cut(s) 320, 525, 1430, 1640, 2219
BseRI GAGGAG 1 cut(s) 819
BsgI GTGCAG 2 cut(s) 531, 2337
BshFI GGCC 6 cut(s) 1286, 1504, 1790, 2021, 2060, 2582
BsiHKAI GWGCWC 5 cut(s) 1116, 1714, 1951, 2282, 2502
BsiSI CCGG 4 cut(s) 1060, 1567, 1907, 2061
BslFI GGGAC 4 cut(s) 295, 401, 1913, 2141
BslI CCNNNNNNNGG 9 cut(s) 338, 339, 500, 725, 1025, 1435, 1646, 2122, 2255
BsmAI GTCTC 6 cut(s) 43, 639, 784, 1035, 1150, 2401
BsmFI GGGAC 4 cut(s) 295, 401, 1913, 2141
BsmI GAATGC 1 cut(s) 2847
BsnI GGCC 6 cut(s) 1286, 1504, 1790, 2021, 2060, 2582
Bso31I GGTCTC 3 cut(s) 43, 784, 1035
Bsp119I TTCGAA 2 cut(s) 509, 2860
Bsp1286I GDGCHC 5 cut(s) 1116, 1714, 1951, 2282, 2502
Bsp13I TCCGGA 2 cut(s) 1059, 1566
Bsp143I GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 2844
BspANI GGCC 6 cut(s) 1286, 1504, 1790, 2021, 2060, 2582
BspCNI CTCAG 3 cut(s) 211, 328, 1359
BspEI TCCGGA 2 cut(s) 1059, 1566
BspLI GGNNCC 4 cut(s) 343, 1058, 2113, 2209
BspMAI CTGCAG 3 cut(s) 2395, 2496, 2764
BspMI ACCTGC 2 cut(s) 553, 2485
BspPI GGATC 4 cut(s) 873, 1330, 1542, 2473
BspQI GCTCTTC 1 cut(s) 1565
BspT104I TTCGAA 2 cut(s) 509, 2860
BspTI CTTAAG 1 cut(s) 302
BspTNI GGTCTC 3 cut(s) 43, 784, 1035
BsrDI GCAATG 4 cut(s) 235, 1710, 2324, 2718
BsrFI RCCGGY 1 cut(s) 2060
BsrI ACTGG 5 cut(s) 320, 525, 1430, 1640, 2219
BssAI RCCGGY 1 cut(s) 2060
BssECI CCNNGG 5 cut(s) 609, 971, 1023, 1335, 1590
BssMI GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
BssNI GRCGYC 2 cut(s) 390, 1926
BssSI CACGAG 1 cut(s) 1713
BssT1I CCWWGG 2 cut(s) 609, 1023
Bst2BI CACGAG 1 cut(s) 1713
Bst2UI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
Bst4CI ACNGT 4 cut(s) 652, 703, 1987, 2050
Bst6I CTCTTC 2 cut(s) 620, 1565
BstACI GRCGYC 2 cut(s) 390, 1926
BstAFI CTTAAG 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 2297, 2315
BstBI TTCGAA 2 cut(s) 509, 2860
BstC8I GCNNGC 8 cut(s) 23, 81, 107, 1186, 2356, 2412, 2584, 2945
BstDEI CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
BstENI CCTNNNNNAGG 1 cut(s) 2120
BstF5I GGATG 4 cut(s) 89, 427, 1563, 1858
BstKTI GATC 7 cut(s) 447, 868, 1325, 1537, 2458, 2468, 2572
BstMAI GTCTC 6 cut(s) 43, 639, 784, 1035, 1150, 2401
BstMBI GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
BstNI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
BstNSI RCATGY 3 cut(s) 25, 1270, 2882
BstSCI CCNGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
BstSFI CTRYAG 7 cut(s) 253, 822, 852, 2391, 2492, 2726, 2760
BstV2I GAAGAC 1 cut(s) 1503
BstX2I RGATCY 3 cut(s) 865, 1322, 2465
BstXI CCANNNNNNTGG 1 cut(s) 2599
BstYI RGATCY 3 cut(s) 865, 1322, 2465
Bsu36I CCTNAGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 6 cut(s) 1286, 1504, 1790, 2021, 2060, 2582
BtgZI GCGATG 3 cut(s) 1777, 2373, 2577
BtrI CACGTC 1 cut(s) 2798
BtsCI GGATG 4 cut(s) 89, 427, 1563, 1858
BtsI GCAGTG 3 cut(s) 897, 2791, 2797
BtsIMutI CAGTG 4 cut(s) 897, 1116, 2791, 2797
BveI ACCTGC 2 cut(s) 553, 2485
Cac8I GCNNGC 8 cut(s) 23, 81, 107, 1186, 2356, 2412, 2584, 2945
CaiI CAGNNNCTG 2 cut(s) 1229, 2627
Cfr10I RCCGGY 1 cut(s) 2060
Cfr13I GGNCC 4 cut(s) 342, 1936, 2081, 2207
CseI GACGC 2 cut(s) 398, 911
Csp6I GTAC 6 cut(s) 299, 606, 1330, 2162, 2702, 2876
CviQI GTAC 6 cut(s) 299, 606, 1330, 2162, 2702, 2876
DdeI CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
DpnI GATC 7 cut(s) 446, 867, 1324, 1536, 2457, 2467, 2571
DpnII GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
DraI TTTAAA 1 cut(s) 2901
EaeI YGGCCR 1 cut(s) 2580
Eam1104I CTCTTC 2 cut(s) 620, 1565
EarI CTCTTC 2 cut(s) 620, 1565
EciI GGCGGA 3 cut(s) 1023, 1111, 1760
Ecl136II GAGCTC 3 cut(s) 1114, 1712, 2280
Eco130I CCWWGG 2 cut(s) 609, 1023
Eco147I AGGCCT 1 cut(s) 1504
Eco24I GRGCYC 3 cut(s) 1116, 1714, 2282
Eco31I GGTCTC 3 cut(s) 43, 784, 1035
Eco47I GGWCC 4 cut(s) 342, 1936, 2081, 2207
Eco53kI GAGCTC 3 cut(s) 1114, 1712, 2280
Eco57I CTGAAG 4 cut(s) 555, 881, 2444, 2468
Eco72I CACGTG 2 cut(s) 2297, 2315
Eco81I CCTNAGG 1 cut(s) 687
EcoICRI GAGCTC 3 cut(s) 1114, 1712, 2280
EcoNI CCTNNNNNAGG 1 cut(s) 2120
EcoO109I RGGNCCY 1 cut(s) 2081
EcoRII CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
EcoT14I CCWWGG 2 cut(s) 609, 1023
EcoT38I GRGCYC 3 cut(s) 1116, 1714, 2282
ErhI CCWWGG 2 cut(s) 609, 1023
FalI AAGNNNNNCTT 4 cut(s) 760, 792, 1452, 1484
FaqI GGGAC 4 cut(s) 295, 401, 1913, 2141
FauI CCCGC 1 cut(s) 76
FokI GGATG 4 cut(s) 76, 434, 1550, 1865
FriOI GRGCYC 3 cut(s) 1116, 1714, 2282
FspBI CTAG 8 cut(s) 402, 485, 582, 603, 1247, 1484, 1493, 1994
GsuI CTGGAG 4 cut(s) 1101, 1190, 1318, 2604
HaeIII GGCC 6 cut(s) 1286, 1504, 1790, 2021, 2060, 2582
HapII CCGG 4 cut(s) 1060, 1567, 1907, 2061
HgaI GACGC 2 cut(s) 398, 911
Hin1I GRCGYC 2 cut(s) 390, 1926
HincII GTYRAC 1 cut(s) 1426
HindII GTYRAC 1 cut(s) 1426
HpaII CCGG 4 cut(s) 1060, 1567, 1907, 2061
HphI GGTGA 8 cut(s) 515, 748, 903, 1738, 1934, 2270, 2509, 2558
Hpy166II GTNNAC 5 cut(s) 592, 911, 1426, 2554, 2795
Hpy8I GTNNAC 5 cut(s) 592, 911, 1426, 2554, 2795
HpyCH4III ACNGT 4 cut(s) 652, 703, 1987, 2050
HpyCH4IV ACGT 4 cut(s) 1926, 2296, 2314, 2797
HpyF3I CTNAG 8 cut(s) 132, 219, 336, 516, 687, 1367, 2117, 2349
HpySE526I ACGT 4 cut(s) 1926, 2296, 2314, 2797
Hsp92I GRCGYC 2 cut(s) 390, 1926
Kpn2I TCCGGA 2 cut(s) 1059, 1566
Kzo9I GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
LguI GCTCTTC 1 cut(s) 1565
LweI GCATC 2 cut(s) 262, 1210
MaeI CTAG 8 cut(s) 402, 485, 582, 603, 1247, 1484, 1493, 1994
MaeII ACGT 4 cut(s) 1926, 2296, 2314, 2797
MalI GATC 7 cut(s) 446, 867, 1324, 1536, 2457, 2467, 2571
MboI GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
MfeI CAATTG 1 cut(s) 2751
MflI RGATCY 3 cut(s) 865, 1322, 2465
MhlI GDGCHC 5 cut(s) 1116, 1714, 1951, 2282, 2502
MlyI GAGTC 7 cut(s) 458, 755, 1197, 1881, 2318, 2330, 2715
MmeI TCCRAC 1 cut(s) 1959
MroI TCCGGA 2 cut(s) 1059, 1566
MslI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
MspA1I CMGCKG 1 cut(s) 659
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 4 cut(s) 1060, 1567, 1907, 2061
MspR9I CCNGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
MunI CAATTG 1 cut(s) 2751
Mva1269I GAATGC 1 cut(s) 2847
MvaI CCWGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
NdeII GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
NlaIV GGNNCC 4 cut(s) 343, 1058, 2113, 2209
NmeAIII GCCGAG 1 cut(s) 2378
NmuCI GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
NspI RCATGY 3 cut(s) 25, 1270, 2882
NspV TTCGAA 2 cut(s) 509, 2860
PaeI GCATGC 1 cut(s) 25
PaqCI CACCTGC 1 cut(s) 2485
PceI AGGCCT 1 cut(s) 1504
PciI ACATGT 1 cut(s) 2878
PciSI GCTCTTC 1 cut(s) 1565
PctI GAATGC 1 cut(s) 2847
PflMI CCANNNNNTGG 1 cut(s) 1646
PfoI TCCNGGA 1 cut(s) 1681
PleI GAGTC 7 cut(s) 457, 754, 1197, 1880, 2317, 2329, 2715
PmaCI CACGTG 2 cut(s) 2297, 2315
PmlI CACGTG 2 cut(s) 2297, 2315
PpsI GAGTC 7 cut(s) 457, 754, 1197, 1880, 2317, 2329, 2715
Ppu21I YACGTR 2 cut(s) 2297, 2315
PpuMI RGGWCCY 1 cut(s) 2081
PscI ACATGT 1 cut(s) 2878
PshBI ATTAAT 1 cut(s) 1302
Psp124BI GAGCTC 3 cut(s) 1116, 1714, 2282
Psp5II RGGWCCY 1 cut(s) 2081
Psp6I CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
PspCI CACGTG 2 cut(s) 2297, 2315
PspGI CCWGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
PspN4I GGNNCC 4 cut(s) 343, 1058, 2113, 2209
PspPI GGNCC 4 cut(s) 342, 1936, 2081, 2207
PspPPI RGGWCCY 1 cut(s) 2081
PstI CTGCAG 3 cut(s) 2395, 2496, 2764
PstNI CAGNNNCTG 2 cut(s) 1229, 2627
PsuI RGATCY 3 cut(s) 865, 1322, 2465
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 6 cut(s) 300, 607, 1331, 2163, 2703, 2877
RsaNI GTAC 6 cut(s) 299, 606, 1330, 2162, 2702, 2876
RseI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
SacI GAGCTC 3 cut(s) 1116, 1714, 2282
SapI GCTCTTC 1 cut(s) 1565
Sau3AI GATC 7 cut(s) 444, 865, 1322, 1534, 2455, 2465, 2569
Sau96I GGNCC 4 cut(s) 342, 1936, 2081, 2207
SbfI CCTGCAGG 1 cut(s) 2496
SchI GAGTC 7 cut(s) 458, 755, 1197, 1881, 2318, 2330, 2715
ScrFI CCNGG 7 cut(s) 972, 1038, 1080, 1208, 1336, 1683, 2622
SdaI CCTGCAGG 1 cut(s) 2496
SduI GDGCHC 5 cut(s) 1116, 1714, 1951, 2282, 2502
SfaNI GCATC 2 cut(s) 262, 1210
SfcI CTRYAG 7 cut(s) 253, 822, 852, 2391, 2492, 2726, 2760
SfuI TTCGAA 2 cut(s) 509, 2860
SinI GGWCC 4 cut(s) 342, 1936, 2081, 2207
SmiMI CAYNNNNRTG 3 cut(s) 228, 1748, 2883
SmlI CTYRAG 3 cut(s) 302, 993, 2505
SmoI CTYRAG 3 cut(s) 302, 993, 2505
SpeI ACTAGT 1 cut(s) 401
SphI GCATGC 1 cut(s) 25
Sse8387I CCTGCAGG 1 cut(s) 2496
SseBI AGGCCT 1 cut(s) 1504
SsiI CCGC 8 cut(s) 83, 356, 1034, 1096, 1196, 1771, 2579, 2844
SspMI CTAG 8 cut(s) 402, 485, 582, 603, 1247, 1484, 1493, 1994
SstI GAGCTC 3 cut(s) 1116, 1714, 2282
StuI AGGCCT 1 cut(s) 1504
StyD4I CCNGG 7 cut(s) 970, 1036, 1078, 1206, 1334, 1681, 2620
StyI CCWWGG 2 cut(s) 609, 1023
TaaI ACNGT 4 cut(s) 652, 703, 1987, 2050
TaiI ACGT 4 cut(s) 1929, 2299, 2317, 2800
TaqI TCGA 6 cut(s) 447, 509, 596, 978, 2472, 2860
TaqII GACCGA 1 cut(s) 184
TatI WGTACW 3 cut(s) 298, 1329, 2875
TauI GCSGC 3 cut(s) 1199, 2582, 2847
TscAI CASTG 4 cut(s) 897, 1123, 2791, 2797
TseFI GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
Tsp45I GTSAC 6 cut(s) 196, 450, 1726, 1922, 2310, 2515
TspDTI ATGAA 7 cut(s) 78, 1320, 1488, 2214, 2277, 2349, 2835
TspRI CASTG 4 cut(s) 897, 1123, 2791, 2797
Van91I CCANNNNNTGG 1 cut(s) 1646
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 4 cut(s) 342, 1936, 2081, 2207
VspI ATTAAT 1 cut(s) 1302
XagI CCTNNNNNAGG 1 cut(s) 2120
XapI RAATTY 4 cut(s) 188, 953, 1314, 2264
XbaI TCTAGA 1 cut(s) 1246
XceI RCATGY 3 cut(s) 25, 1270, 2882
XspI CTAG 8 cut(s) 402, 485, 582, 603, 1247, 1484, 1493, 1994
ZraI GACGTC 1 cut(s) 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.