FvH4_3g18830

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010541
Physical Location & Seq
Forward (+)
12091584 .. 12095505
3922 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g18830.t1

Sequence Viewer

Length: 2625 bp
ATGAATAAGTTTTCTCCTCTAATTTATTTTGTTATCATAGTTCTAGCATTTAGACCAGTTACTCCCAGTGCAAAGAGAAATATTGAAGCAGCTGGAAGTACGGGCGTCGTTTTTAACATTGGGGCAATTATAGATAATTGTTCTCGAATTGGCAAAGAAGAGAGAGTGGCAATGGAAATGGCAATGGATGATTTTTATGCCAATAACAATCAGAGATTTGTTCTGCAAATTAGACACTCTCAGAGGGACCCCATGCAGGCGGCTCTTGCAGCTAGAGATCTCATAGATATGCAAGACGTTCAAGCCATTCTAGGACCACGTACATGGGAGGATACATCATTAGTTGCTGAGGTTGGCAGTGAGAATCACATCCCAATTGTTTCTTTTGCCGAGGCTACTCCAAACTGGGCAACAGAGCTCTGGCCCTCCCTAGTTCAAGCTTCTCATGATGAGCTAAAACAAATGGAAGCAATAGCTGCTATAGTTAAGTCATTTGAATGGCATCAAGCCACTGTTATATACCAAGATAGAGATTCTTCATCCAACGAAATTTTGCCTCGCCTCTCTCTTGCCTTGAGACAAGTTGGTGCAGAAATTAGCCAGCTCGTACCTCTTCCACCTTTCGGCTCTTCTTCATTAGTCAAAGCGCTTGAGAAGCTTCGACAAGACCAGTGTAGAGTTTTCATCGTTCATTTGGCTTTGCCGTTGGCATTGCAACTATTTGATAAGGCTAAAGAAATGAAGATGATGGAGAAAGACTATGTATGGATCACCACAGATCCTATTACTAGCCTAGTCCATTCCTTCAATGCCTCCACCATTTCCACAATGCAAGGGGTTATCGGAGTCAAGAGCTACTTCCCGGAAAGTGAGAGTAGCCAATTTCACGGGTTTCGTCGAAGATTTCGTAAAAGGTTCAGCTCAGAATATCCTGAAGAGGAAAACGATGAACCTGAGGTCTTCGCAGCACAGGCCTATGATGCTGCAACGGCAGTGGCTCTAGCAGTGACACAAAGAAAACATAATGGGAAGCCCTTGTTGAAAAATATTCTTCAAATTGATTTTCATGGTTTAAGTGGCAAAATTCAGTTCACTGACAAAAAATTACCACCACAACAAATGTTCCAGATTATCAATGTGATCGGAAAGAGTTACATAGAACTCGGATATTGGTCAGATGGATTAGGCTTCAGACGGACTGTCGGGGGAAAAAATACAATCGGCTCATCCATGAAAGATTTTGGACCAGTGTTTTGGCCAGGGGGGCCTCTCTATACTCCAAAAGGATGGACTGTATCAACAAGTTCAAAGCCATTGAAAATCGGCGTGCCTACCGGATCAACATTCAAACAGTATGTAAATGCAGAAAAAGATCCCTCGGGAAATAATTTCTCATTCCGTGGGGTTGCAATTGATCTCTTCGAAGAAACCCTGATGGAGCTGCCTTATCATTTGCCATATGATTTCTTTCCATTTGATGGCACATATGATGCTATAGTGCAGCAAATTTATTCTAGAGAATTCGATGCTGTAGTTGGTGATGTAGCAATAGTGTCGCGAAGATGTCAGTATGCAGAATTCACACACCCCTATTCTGAAGCAGGATTGATGATGATAGTTCCAGTCAGGTCCAAAATTTGCAATAAAGCATGGTTATTCATGAAGCCTTTCACAGAAGCCATGTGGGCGCTCATAGTCGCCATCAATGTCTACAATGGTTTTGTTGTCTGGTTGATAGAACGAAACCACTGCCCTGAACTCAGAGGTTCTCTGCCAAATCAAATTGGAACCTTGATTTGGCTTTCCTTCACCACTCTATTCCCCTTGCATGGAGAAAAGCTGCATAGCAATCTATCAAGGATGACAATGGTTGTCTGGTTATTTGTCGCACTAATCATCACACAGACGTACACCGCCAACCTCACCAGCATGCTCACGGTGCAGCTACTTGAACCAGCTGTAGCAGATGTTGATGCACTTCTACAGAGCAATGCCGTTGTTGGTTATTGTAAAGGATCCTTTGTTTCTATGTATCTGAATGAAGTCTTGCAATTTCCCCGTAATCGCATTCAGGAGTTTAGCTCTCCGGAAGAGTATGCAAACGCCCTCAAAAGCAAAGCAATTGCTGCTGCTTTTCTTGAAGTCCCTTTTGCCAAGATATTCCTCGGACAATACTGCAAAGATTTTGTGTCGGTCGGGCCAACATACAAAGTCGGAGGATTTGGATTCGCATTTCCAAGGGGGTCACCATTACTTTCTAGTGTAAATGAAGCAATGCTGAAGGTGACCGAAAGTGGTAAGCTTCGACAGTTGGAGAACAACATGCTGGCAACTCAGAAATGCTTGGATGCAGAAACAGATGGTAGTACCCCAAGTCTAAGCCCCAGCAGCTTTTGGATACTATTCATATTCTCAGGAGGGACATCAACCATTTCTCTTGGGGTTTACATTTTCCATATTTATAAATCGATGTTTAAGCAGGAAACAGTCTGGAAGTTAATGTTGGCTGTAATCAACCAATGTTTGACCCCAACTATGCAGAGGTTCTCAAGAAGAGATAGTGACATTAATCCAGAAACTCCGACGAATTATGCAAATGGCCCTAACTTGCAGGCTCTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

875

Amino Acids

97.96

Weight (kDa)

6.47

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 50 - 375 8.3e-26 Periplasmic binding protein
ANF_receptor PF01094 55 - 382 1.3e-56 Receptor family ligand binding region
SBP_bac_3 PF00497 465 - 773 1.2e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 560 - 805 4.1e-24 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2463
AccB7I CCANNNNNTGG 1 cut(s) 1478
AccI GTMKAC 1 cut(s) 1710
AccII CGCG 1 cut(s) 1558
AccIII TCCGGA 1 cut(s) 2086
AciI CCGC 2 cut(s) 260, 1914
AclWI GGATC 6 cut(s) 773, 776, 1345, 1367, 2010, 2023
AcoI YGGCCR 1 cut(s) 1256
AcsI RAATTY 6 cut(s) 549, 1083, 1506, 1520, 1577, 1635
AcuI CTGAAG 4 cut(s) 954, 1174, 1617, 2300
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 5 cut(s) 100, 322, 609, 1910, 2368
AfeI AGCGCT 1 cut(s) 648
AfiI CCNNNNNNNGG 5 cut(s) 256, 623, 1478, 1797, 1829
AhdI GACNNNNNGTC 1 cut(s) 1199
AjnI CCWGG 1 cut(s) 1258
AjuI GAANNNNNNNTTGG 4 cut(s) 1780, 1812, 2486, 2518
Alw21I GWGCWC 1 cut(s) 420
Alw26I GTCTC 1 cut(s) 571
AlwI GGATC 6 cut(s) 773, 776, 1345, 1367, 2010, 2023
AlwNI CAGNNNCTG 1 cut(s) 2617
Ama87I CYCGRG 1 cut(s) 1378
Aor13HI TCCGGA 1 cut(s) 2086
Aor51HI AGCGCT 1 cut(s) 648
AoxI GGCC 6 cut(s) 422, 972, 1256, 1265, 2198, 2599
ApoI RAATTY 6 cut(s) 549, 1083, 1506, 1520, 1577, 1635
ArsI GACNNNNNNTTYG 2 cut(s) 1236, 1268
AseI ATTAAT 1 cut(s) 2568
Asp700I GAANNNNTTC 1 cut(s) 1667
AspLEI GCGC 2 cut(s) 649, 1690
AspS9I GGNCC 8 cut(s) 247, 314, 423, 1244, 1265, 1629, 2198, 2600
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 6 cut(s) 763, 1550, 1801, 1915, 2238, 2296
AsuII TTCGAA 1 cut(s) 1422
AvaI CYCGRG 1 cut(s) 1378
AvaII GGWCC 4 cut(s) 247, 314, 1244, 1629
AxyI CCTNAGG 1 cut(s) 954
BalI TGGCCA 1 cut(s) 1258
BamHI GGATCC 1 cut(s) 2015
BanII GRGCYC 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 952
Bbv12I GWGCWC 1 cut(s) 420
BbvCI CCTCAGC 1 cut(s) 348
BccI CCATC 7 cut(s) 742, 1172, 1281, 1429, 1472, 1709, 2354
BceAI ACGGC 3 cut(s) 688, 1005, 1979
BcgI CGANNNNNNTGC 4 cut(s) 1536, 1570, 1731, 1765
BciT130I CCWGG 1 cut(s) 1260
BciVI GTATCC 2 cut(s) 325, 2391
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 9 cut(s) 44, 273, 311, 431, 789, 794, 1001, 1515, 2259
BfmI CTRYAG 5 cut(s) 480, 1494, 1530, 1959, 1982
BfoI RGCGCY 2 cut(s) 650, 1691
BfuI GTATCC 2 cut(s) 325, 2391
BglI GCCNNNNNGGC 2 cut(s) 1264, 1685
BglII AGATCT 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 863, 1260
Bme18I GGWCC 4 cut(s) 247, 314, 1244, 1629
BmeRI GACNNNNNGTC 1 cut(s) 1199
BmeT110I CYCGRG 1 cut(s) 1378
BmgT120I GGNCC 8 cut(s) 247, 314, 423, 1244, 1265, 1629, 2198, 2600
BmiI GGNNCC 5 cut(s) 248, 249, 1266, 1789, 2017
BmrFI CCNGG 2 cut(s) 863, 1260
BmrI ACTGGG 2 cut(s) 60, 415
BmsI GCATC 6 cut(s) 511, 970, 1480, 1516, 1963, 2338
BmuI ACTGGG 2 cut(s) 60, 415
BpiI GAAGAC 1 cut(s) 952
BplI GAGNNNNNCTC 2 cut(s) 2066, 2098
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 1422
BpuEI CTTGAG 3 cut(s) 595, 671, 2533
BpuMI CCSGG 1 cut(s) 863
Bsa29I ATCGAT 1 cut(s) 2468
BsaAI YACGTR 1 cut(s) 320
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 390, 1259, 1377, 1399, 2164, 2237
BsaWI WCCGGW 2 cut(s) 1334, 2086
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 623, 1478, 1797, 1829
Bse1I ACTGG 6 cut(s) 56, 66, 410, 670, 1247, 1622
Bse21I CCTNAGG 1 cut(s) 954
Bse3DI GCAATG 5 cut(s) 177, 189, 710, 1996, 2280
BseAI TCCGGA 1 cut(s) 2086
BseBI CCWGG 1 cut(s) 1260
BseCI ATCGAT 1 cut(s) 2468
BseDI CCNNGG 6 cut(s) 390, 1259, 1377, 1399, 2164, 2237
BseGI GGATG 7 cut(s) 193, 369, 539, 1226, 1292, 1866, 2353
BseLI CCNNNNNNNGG 5 cut(s) 256, 623, 1478, 1797, 1829
BseMI GCAATG 5 cut(s) 177, 189, 710, 1996, 2280
BseMII CTCAG 7 cut(s) 254, 339, 936, 945, 1774, 2348, 2427
BseNI ACTGG 6 cut(s) 56, 66, 410, 670, 1247, 1622
BseRI GAGGAG 1 cut(s) 6
BseYI CCCAGC 1 cut(s) 2384
BsgI GTGCAG 3 cut(s) 609, 1520, 1961
Bsh1236I CGCG 1 cut(s) 1558
Bsh1285I CGRYCG 1 cut(s) 2196
BshFI GGCC 6 cut(s) 424, 974, 1258, 1267, 2200, 2601
BshVI ATCGAT 1 cut(s) 2468
BsiEI CGRYCG 1 cut(s) 2196
BsiHKAI GWGCWC 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 1378
BsiSI CCGG 3 cut(s) 863, 1335, 2087
BslFI GGGAC 3 cut(s) 260, 2129, 2434
BslI CCNNNNNNNGG 5 cut(s) 256, 623, 1478, 1797, 1829
BsmAI GTCTC 1 cut(s) 571
BsmFI GGGAC 3 cut(s) 260, 2129, 2434
BsmI GAATGC 1 cut(s) 2067
BsnI GGCC 6 cut(s) 424, 974, 1258, 1267, 2200, 2601
BsoBI CYCGRG 1 cut(s) 1378
Bsp119I TTCGAA 1 cut(s) 1422
Bsp1286I GDGCHC 1 cut(s) 420
Bsp13I TCCGGA 1 cut(s) 2086
Bsp143I GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
Bsp68I TCGCGA 1 cut(s) 1558
BspACI CCGC 2 cut(s) 260, 1914
BspANI GGCC 6 cut(s) 424, 974, 1258, 1267, 2200, 2601
BspCNI CTCAG 7 cut(s) 253, 340, 935, 946, 1773, 2347, 2426
BspDI ATCGAT 1 cut(s) 2468
BspEI TCCGGA 1 cut(s) 2086
BspFNI CGCG 1 cut(s) 1558
BspHI TCATGA 2 cut(s) 445, 1659
BspLI GGNNCC 5 cut(s) 248, 249, 1266, 1789, 2017
BspPI GGATC 6 cut(s) 773, 776, 1345, 1367, 2010, 2023
BspQI GCTCTTC 1 cut(s) 634
BspT104I TTCGAA 1 cut(s) 1422
BsrDI GCAATG 5 cut(s) 177, 189, 710, 1996, 2280
BsrI ACTGG 6 cut(s) 56, 66, 410, 670, 1247, 1622
BssECI CCNNGG 6 cut(s) 390, 1259, 1377, 1399, 2164, 2237
BssMI GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 2237
Bst2UI CCWGG 1 cut(s) 1260
Bst4CI ACNGT 7 cut(s) 514, 1201, 1294, 1353, 1939, 2310, 2488
Bst6I CTCTTC 7 cut(s) 153, 618, 634, 930, 1424, 2085, 2548
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 2 cut(s) 476, 2126
BstBAI YACGTR 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 1422
BstC8I GCNNGC 6 cut(s) 258, 602, 1328, 1931, 2328, 2613
BstDEI CTNAG 8 cut(s) 240, 348, 922, 954, 1760, 2334, 2378, 2413
BstDSI CCRYGG 1 cut(s) 1399
BstEII GGTNACC 2 cut(s) 2244, 2284
BstF5I GGATG 7 cut(s) 193, 369, 539, 1226, 1292, 1866, 2353
BstFNI CGCG 1 cut(s) 1558
BstH2I RGCGCY 2 cut(s) 650, 1691
BstHHI GCGC 2 cut(s) 649, 1690
BstKTI GATC 8 cut(s) 280, 771, 781, 1143, 1340, 1375, 1417, 2018
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
BstMCI CGRYCG 1 cut(s) 2196
BstNI CCWGG 1 cut(s) 1260
BstNSI RCATGY 2 cut(s) 1933, 2326
BstPI GGTNACC 2 cut(s) 2244, 2284
BstSCI CCNGG 2 cut(s) 861, 1258
BstSFI CTRYAG 5 cut(s) 480, 1494, 1530, 1959, 1982
BstUI CGCG 1 cut(s) 1558
BstV2I GAAGAC 1 cut(s) 952
BstX2I RGATCY 4 cut(s) 277, 778, 1372, 2015
BstXI CCANNNNNNTGG 3 cut(s) 324, 1254, 1287
BstYI RGATCY 4 cut(s) 277, 778, 1372, 2015
Bsu15I ATCGAT 1 cut(s) 2468
Bsu36I CCTNAGG 1 cut(s) 954
BsuI GTATCC 2 cut(s) 325, 2391
BsuRI GGCC 6 cut(s) 424, 974, 1258, 1267, 2200, 2601
BsuTUI ATCGAT 1 cut(s) 2468
BtgI CCRYGG 1 cut(s) 1399
BtsCI GGATG 7 cut(s) 193, 369, 539, 1226, 1292, 1866, 2353
BtsI GCAGTG 4 cut(s) 364, 999, 1011, 1747
BtsIMutI CAGTG 9 cut(s) 73, 364, 510, 677, 999, 1011, 1092, 1254, 1747
BtuMI TCGCGA 1 cut(s) 1558
Cac8I GCNNGC 6 cut(s) 258, 602, 1328, 1931, 2328, 2613
CaiI CAGNNNCTG 1 cut(s) 2617
CciI TCATGA 2 cut(s) 445, 1659
CfoI GCGC 2 cut(s) 649, 1690
Cfr13I GGNCC 8 cut(s) 247, 314, 423, 1244, 1265, 1629, 2198, 2600
ClaI ATCGAT 1 cut(s) 2468
CseI GACGC 1 cut(s) 94
Csp6I GTAC 5 cut(s) 99, 321, 608, 1909, 2367
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviQI GTAC 5 cut(s) 99, 321, 608, 1909, 2367
DdeI CTNAG 8 cut(s) 240, 348, 922, 954, 1760, 2334, 2378, 2413
DpnI GATC 8 cut(s) 279, 770, 780, 1142, 1339, 1374, 1416, 2017
DpnII GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
DriI GACNNNNNGTC 1 cut(s) 1199
EaeI YGGCCR 1 cut(s) 1256
Eam1104I CTCTTC 7 cut(s) 153, 618, 634, 930, 1424, 2085, 2548
Eam1105I GACNNNNNGTC 1 cut(s) 1199
EarI CTCTTC 7 cut(s) 153, 618, 634, 930, 1424, 2085, 2548
Ecl136II GAGCTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 2237
Eco147I AGGCCT 1 cut(s) 974
Eco24I GRGCYC 1 cut(s) 420
Eco47I GGWCC 4 cut(s) 247, 314, 1244, 1629
Eco47III AGCGCT 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 4 cut(s) 954, 1174, 1617, 2300
Eco81I CCTNAGG 1 cut(s) 954
Eco88I CYCGRG 1 cut(s) 1378
Eco91I GGTNACC 2 cut(s) 2244, 2284
EcoICRI GAGCTC 1 cut(s) 418
EcoO109I RGGNCCY 2 cut(s) 247, 1265
EcoO65I GGTNACC 2 cut(s) 2244, 2284
EcoRI GAATTC 2 cut(s) 1520, 1577
EcoRII CCWGG 1 cut(s) 1258
EcoT14I CCWWGG 1 cut(s) 2237
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 2237
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FaqI GGGAC 3 cut(s) 260, 2129, 2434
FauNDI CATATG 2 cut(s) 1459, 1486
FblI GTMKAC 1 cut(s) 1710
FokI GGATG 7 cut(s) 200, 356, 526, 1213, 1299, 1873, 2360
FriOI GRGCYC 1 cut(s) 420
FspBI CTAG 9 cut(s) 44, 273, 311, 431, 789, 794, 1001, 1515, 2259
GlaI GCGC 2 cut(s) 648, 1689
GsaI CCCAGC 1 cut(s) 2388
HaeII RGCGCY 2 cut(s) 650, 1691
HaeIII GGCC 6 cut(s) 424, 974, 1258, 1267, 2200, 2601
HapII CCGG 3 cut(s) 863, 1335, 2087
HgaI GACGC 1 cut(s) 94
HhaI GCGC 2 cut(s) 649, 1690
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 2 cut(s) 647, 1688
HinP1I GCGC 2 cut(s) 647, 1688
HindIII AAGCTT 3 cut(s) 438, 656, 2300
HinfI GANTC 4 cut(s) 364, 533, 846, 2226
HpaII CCGG 3 cut(s) 863, 1335, 2087
HphI GGTGA 6 cut(s) 763, 1550, 1801, 1915, 2238, 2296
Hpy166II GTNNAC 4 cut(s) 1092, 1711, 1911, 2446
Hpy8I GTNNAC 4 cut(s) 1092, 1711, 1911, 2446
Hpy99I CGWCG 3 cut(s) 110, 900, 2587
HpyAV CCTTC 3 cut(s) 814, 1816, 2275
HpyCH4III ACNGT 7 cut(s) 514, 1201, 1294, 1353, 1939, 2310, 2488
HpyCH4IV ACGT 3 cut(s) 297, 319, 1907
HpyF3I CTNAG 8 cut(s) 240, 348, 922, 954, 1760, 2334, 2378, 2413
HpySE526I ACGT 3 cut(s) 297, 319, 1907
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 2 cut(s) 647, 1688
KflI GGGWCCC 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 2086
Kzo9I GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
LguI GCTCTTC 1 cut(s) 634
LmnI GCTCC 1 cut(s) 1438
LweI GCATC 6 cut(s) 511, 970, 1480, 1516, 1963, 2338
MaeI CTAG 9 cut(s) 44, 273, 311, 431, 789, 794, 1001, 1515, 2259
MaeII ACGT 3 cut(s) 297, 319, 1907
MaeIII GTNAC 6 cut(s) 58, 1006, 1151, 2244, 2284, 2561
MalI GATC 8 cut(s) 279, 770, 780, 1142, 1339, 1374, 1416, 2017
MboI GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
MfeI CAATTG 3 cut(s) 375, 1410, 2121
MflI RGATCY 4 cut(s) 277, 778, 1372, 2015
MhlI GDGCHC 1 cut(s) 420
MlsI TGGCCA 1 cut(s) 1258
MluNI TGGCCA 1 cut(s) 1258
MlyI GAGTC 1 cut(s) 855
MmeI TCCRAC 4 cut(s) 567, 2194, 2292, 2606
Mox20I TGGCCA 1 cut(s) 1258
MroI TCCGGA 1 cut(s) 2086
MroXI GAANNNNTTC 1 cut(s) 1667
MscI TGGCCA 1 cut(s) 1258
MseI TTAA 6 cut(s) 114, 486, 1073, 2475, 2498, 2568
MslI CAYNNNNRTG 4 cut(s) 287, 322, 496, 1928
Msp20I TGGCCA 1 cut(s) 1258
MspA1I CMGCKG 2 cut(s) 92, 1958
MspI CCGG 3 cut(s) 863, 1335, 2087
MspR9I CCNGG 2 cut(s) 863, 1260
MunI CAATTG 3 cut(s) 375, 1410, 2121
Mva1269I GAATGC 1 cut(s) 2067
MvaI CCWGG 1 cut(s) 1260
MvnI CGCG 1 cut(s) 1558
NciI CCSGG 1 cut(s) 863
NdeI CATATG 2 cut(s) 1459, 1486
NdeII GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
NlaIV GGNNCC 5 cut(s) 248, 249, 1266, 1789, 2017
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 4 cut(s) 1006, 2244, 2284, 2561
NruI TCGCGA 1 cut(s) 1558
NspI RCATGY 2 cut(s) 1933, 2326
NspV TTCGAA 1 cut(s) 1422
PaeI GCATGC 1 cut(s) 1933
PagI TCATGA 2 cut(s) 445, 1659
PceI AGGCCT 1 cut(s) 974
PciSI GCTCTTC 1 cut(s) 634
PcsI WCGNNNNNNNCGW 1 cut(s) 904
PctI GAATGC 1 cut(s) 2067
PdmI GAANNNNTTC 1 cut(s) 1667
PfeI GAWTC 3 cut(s) 364, 533, 2226
PflMI CCANNNNNTGG 1 cut(s) 1478
PfoI TCCNGGA 1 cut(s) 861
PleI GAGTC 1 cut(s) 854
PpsI GAGTC 1 cut(s) 854
Ppu21I YACGTR 1 cut(s) 320
PpuMI RGGWCCY 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 2568
PsiI TTATAA 1 cut(s) 2463
Psp124BI GAGCTC 1 cut(s) 420
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 1258
PspEI GGTNACC 2 cut(s) 2244, 2284
PspFI CCCAGC 1 cut(s) 2384
PspGI CCWGG 1 cut(s) 1258
PspN4I GGNNCC 5 cut(s) 248, 249, 1266, 1789, 2017
PspPI GGNCC 8 cut(s) 247, 314, 423, 1244, 1265, 1629, 2198, 2600
PspPPI RGGWCCY 1 cut(s) 247
PstNI CAGNNNCTG 1 cut(s) 2617
PsuI RGATCY 4 cut(s) 277, 778, 1372, 2015
PvuII CAGCTG 2 cut(s) 92, 1958
RruI TCGCGA 1 cut(s) 1558
RsaI GTAC 5 cut(s) 100, 322, 609, 1910, 2368
RsaNI GTAC 5 cut(s) 99, 321, 608, 1909, 2367
RseI CAYNNNNRTG 4 cut(s) 287, 322, 496, 1928
SacI GAGCTC 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 634
SaqAI TTAA 6 cut(s) 114, 486, 1073, 2475, 2498, 2568
Sau3AI GATC 8 cut(s) 277, 768, 778, 1140, 1337, 1372, 1414, 2015
Sau96I GGNCC 8 cut(s) 247, 314, 423, 1244, 1265, 1629, 2198, 2600
SchI GAGTC 1 cut(s) 855
ScrFI CCNGG 2 cut(s) 863, 1260
SduI GDGCHC 1 cut(s) 420
SfaNI GCATC 6 cut(s) 511, 970, 1480, 1516, 1963, 2338
SfcI CTRYAG 5 cut(s) 480, 1494, 1530, 1959, 1982
SfiI GGCCNNNNNGGCC 1 cut(s) 1264
SfuI TTCGAA 1 cut(s) 1422
SinI GGWCC 4 cut(s) 247, 314, 1244, 1629
SmiMI CAYNNNNRTG 4 cut(s) 287, 322, 496, 1928
SmlI CTYRAG 3 cut(s) 574, 650, 2548
SmoI CTYRAG 3 cut(s) 574, 650, 2548
SphI GCATGC 1 cut(s) 1933
SseBI AGGCCT 1 cut(s) 974
SsiI CCGC 2 cut(s) 260, 1914
SspI AATATT 2 cut(s) 82, 1048
SspMI CTAG 9 cut(s) 44, 273, 311, 431, 789, 794, 1001, 1515, 2259
SstI GAGCTC 1 cut(s) 420
StuI AGGCCT 1 cut(s) 974
StyD4I CCNGG 2 cut(s) 861, 1258
StyI CCWWGG 1 cut(s) 2237
TaaI ACNGT 7 cut(s) 514, 1201, 1294, 1353, 1939, 2310, 2488
TaiI ACGT 3 cut(s) 300, 322, 1910
TaqI TCGA 7 cut(s) 145, 661, 898, 1422, 1524, 2305, 2468
TaqII GACCGA 2 cut(s) 2182, 2303
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 3 cut(s) 364, 533, 2226
Tru1I TTAA 6 cut(s) 114, 486, 1073, 2475, 2498, 2568
Tru9I TTAA 6 cut(s) 114, 486, 1073, 2475, 2498, 2568
TscAI CASTG 9 cut(s) 73, 364, 517, 677, 999, 1011, 1099, 1254, 1754
TseFI GTSAC 4 cut(s) 1006, 2244, 2284, 2561
Tsp45I GTSAC 4 cut(s) 1006, 2244, 2284, 2561
TspGWI ACGGA 2 cut(s) 1210, 1388
TspRI CASTG 9 cut(s) 73, 364, 517, 677, 999, 1011, 1099, 1254, 1754
Van91I CCANNNNNTGG 1 cut(s) 1478
VpaK11BI GGWCC 4 cut(s) 247, 314, 1244, 1629
VspI ATTAAT 1 cut(s) 2568
XapI RAATTY 6 cut(s) 549, 1083, 1506, 1520, 1577, 1635
XbaI TCTAGA 1 cut(s) 1514
XceI RCATGY 2 cut(s) 1933, 2326
XmiI GTMKAC 1 cut(s) 1710
XmnI GAANNNNTTC 1 cut(s) 1667
XspI CTAG 9 cut(s) 44, 273, 311, 431, 789, 794, 1001, 1515, 2259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.