MD03G1237700.v1.1

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010541
Physical Location & Seq
Reverse (-)
32284038 .. 32285754
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1237700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGACAAGTTTCCTGCTTTGTTGAGCTTCATGTCCTTTGTTTTACTATTTACACAAAAGTTGAGAGCTGATGGAAGTAGCGACACAATAAGGGACAATGTTTTCGTAGGCATAGGTGCCATCGTAGAAACCGGTTCTCGAGTTGGTAAAGAGCAGAGAGTAGCAATGACTTTAACACACGCAACAATCAAAGTTTGGTTTTTGCACATGAGAAACTCTAAAGGGGAGCTTCTACAGGCAGCTCTAGCAGCCAAAGATCTTAACAGTATGGATCAATTGCGAGCAATTCTTGGACTACAGACGTGGGAAGAGGTGTTGTTAGTCACTGAGATTGGAACTCAGAGTCATGTTCCAATTATTTCTTTAGCTGATGCAACTCCAAAGTGGGCAACTGAGCAGTGGAGCTTTCTAGTTCAAGCTTCACCAAATCAGTTAAAGCAGATCGAAGCTGTAGCAGCTATGATTCAATTTTGGGAGTGGAATCAAGTCACCATTACAGTCTGTATGCTAACAGTTACAAGTCATCTATCCAAGGCTCTTAAACATGTAGGCGCAGAAATTGTCCATTATGTAGCTTTCGCACCTTTTGCTTCTTCTTCATTGTCTCATCAGCTCGATAGGGTGTTTGTTGTCCACCTGTCTTTAGCAGCGGCGGTGCAGCTATTTGAGAGGGCAAAAAGAATGAAACTAATGAAAAAGGATTGTCTGGATCGCCACCGATCCCTTTACAAGCCTTGTCCATTCTTTCAATGCCACTACCATCTCCTTAATGCAAGGAATTATTGGAGTCAAAAGTTACGTTCACAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

271

Amino Acids

30.62

Weight (kDa)

9.49

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 68 - 233 6.3e-15 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 2 cut(s) 650, 653
AclWI GGATC 3 cut(s) 279, 714, 717
AflIII ACRYGT 1 cut(s) 544
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 3 cut(s) 416, 467, 749
AjiI CACGTC 1 cut(s) 303
Alw26I GTCTC 1 cut(s) 609
AlwI GGATC 3 cut(s) 279, 714, 717
Ama87I CYCGRG 1 cut(s) 138
ApeKI GCWGC 5 cut(s) 239, 248, 455, 647, 658
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AsiGI ACCGGT 1 cut(s) 131
AspLEI GCGC 1 cut(s) 554
AsuHPI GGTGA 2 cut(s) 414, 481
AvaI CYCGRG 1 cut(s) 138
BanI GGYRCC 1 cut(s) 116
BbvI GCAGC 5 cut(s) 251, 260, 467, 659, 670
BccI CCATC 3 cut(s) 65, 128, 768
BcoDI GTCTC 1 cut(s) 609
BfaI CTAG 2 cut(s) 245, 410
BfmI CTRYAG 3 cut(s) 233, 296, 450
BglII AGATCT 1 cut(s) 256
BisI GCNGC 6 cut(s) 240, 249, 456, 648, 651, 659
BlsI GCNGC 6 cut(s) 241, 250, 457, 649, 652, 660
BmeT110I CYCGRG 1 cut(s) 138
BmgBI CACGTC 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 1 cut(s) 361
BsaJI CCNNGG 1 cut(s) 531
BsaWI WCCGGW 1 cut(s) 131
Bse118I RCCGGY 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 171
BseDI CCNNGG 1 cut(s) 531
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 3 cut(s) 318, 353, 384
BseXI GCAGC 5 cut(s) 251, 260, 467, 659, 670
BsgI GTGCAG 1 cut(s) 677
BshNI GGYRCC 1 cut(s) 116
BshTI ACCGGT 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 138
Bsp143I GATC 5 cut(s) 256, 271, 441, 709, 719
BspACI CCGC 2 cut(s) 650, 653
BspCNI CTCAG 3 cut(s) 319, 352, 385
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 3 cut(s) 279, 714, 717
BspT107I GGYRCC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 531
BssMI GATC 5 cut(s) 256, 271, 441, 709, 719
BssT1I CCWWGG 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 266, 499, 514
Bst6I CTCTTC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 3 cut(s) 327, 339, 393
BstHHI GCGC 1 cut(s) 554
BstKTI GATC 5 cut(s) 259, 274, 444, 712, 722
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 5 cut(s) 256, 271, 441, 709, 719
BstMWI GCNNNNNNNGC 4 cut(s) 245, 248, 455, 587
BstNSI RCATGY 1 cut(s) 548
BstSFI CTRYAG 3 cut(s) 233, 296, 450
BstV1I GCAGC 5 cut(s) 251, 260, 467, 659, 670
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BtrI CACGTC 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 404
BtsIMutI CAGTG 2 cut(s) 324, 404
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 1 cut(s) 554
Cfr10I RCCGGY 1 cut(s) 131
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 4 cut(s) 31, 208, 347, 545
DdeI CTNAG 3 cut(s) 327, 339, 393
DpnI GATC 5 cut(s) 258, 273, 443, 711, 721
DpnII GATC 5 cut(s) 256, 271, 441, 709, 719
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 4 cut(s) 34, 211, 350, 548
FaiI YATR 9 cut(s) 32, 113, 209, 269, 348, 461, 506, 546, 570
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 107
FatI CATG 4 cut(s) 30, 207, 346, 544
Fnu4HI GCNGC 6 cut(s) 240, 249, 456, 648, 651, 659
Fsp4HI GCNGC 6 cut(s) 240, 249, 456, 648, 651, 659
FspBI CTAG 2 cut(s) 245, 410
GlaI GCGC 1 cut(s) 553
GluI GCNGC 6 cut(s) 240, 249, 456, 648, 651, 659
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 554
Hin1II CATG 4 cut(s) 34, 211, 350, 548
Hin6I GCGC 1 cut(s) 552
HinP1I GCGC 1 cut(s) 552
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 4 cut(s) 343, 463, 481, 787
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 414, 481
Hpy166II GTNNAC 2 cut(s) 634, 803
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 2 cut(s) 138, 707
Hpy8I GTNNAC 2 cut(s) 634, 803
HpyCH4III ACNGT 3 cut(s) 266, 499, 514
HpyCH4IV ACGT 2 cut(s) 302, 799
HpyCH4V TGCA 4 cut(s) 205, 374, 658, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 248, 455, 587
HpyF3I CTNAG 3 cut(s) 327, 339, 393
HpySE526I ACGT 2 cut(s) 302, 799
Hsp92II CATG 4 cut(s) 34, 211, 350, 548
HspAI GCGC 1 cut(s) 552
Kzo9I GATC 5 cut(s) 256, 271, 441, 709, 719
LmnI GCTCC 2 cut(s) 226, 402
LpnPI CCDG 5 cut(s) 27, 145, 221, 650, 692
Lsp1109I GCAGC 5 cut(s) 251, 260, 467, 659, 670
LweI GCATC 1 cut(s) 361
MaeI CTAG 2 cut(s) 245, 410
MaeII ACGT 2 cut(s) 302, 799
MaeIII GTNAC 4 cut(s) 322, 487, 514, 795
MalI GATC 5 cut(s) 258, 273, 443, 711, 721
MboI GATC 5 cut(s) 256, 271, 441, 709, 719
MboII GAAGA 3 cut(s) 320, 585, 588
MfeI CAATTG 1 cut(s) 275
MflI RGATCY 1 cut(s) 256
MluCI AATT 6 cut(s) 275, 285, 354, 467, 558, 778
MlyI GAGTC 2 cut(s) 352, 796
MnlI CCTC 2 cut(s) 304, 663
MseI TTAA 5 cut(s) 173, 261, 434, 540, 768
MslI CAYNNNNRTG 1 cut(s) 808
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 1 cut(s) 132
MunI CAATTG 1 cut(s) 275
MwoI GCNNNNNNNGC 4 cut(s) 245, 248, 455, 587
NdeII GATC 5 cut(s) 256, 271, 441, 709, 719
NlaIII CATG 4 cut(s) 34, 211, 350, 548
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 2 cut(s) 322, 487
NspI RCATGY 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 138
PciI ACATGT 1 cut(s) 544
PfeI GAWTC 2 cut(s) 463, 481
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 6 cut(s) 241, 250, 457, 649, 652, 660
PleI GAGTC 2 cut(s) 351, 795
PpsI GAGTC 2 cut(s) 351, 795
PscI ACATGT 1 cut(s) 544
PspN4I GGNNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 808
SaqAI TTAA 5 cut(s) 173, 261, 434, 540, 768
SatI GCNGC 6 cut(s) 240, 249, 456, 648, 651, 659
Sau3AI GATC 5 cut(s) 256, 271, 441, 709, 719
SchI GAGTC 2 cut(s) 352, 796
SfaNI GCATC 1 cut(s) 361
SfcI CTRYAG 3 cut(s) 233, 296, 450
Sfr274I CTCGAG 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 808
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 6 cut(s) 275, 285, 354, 467, 558, 778
SsiI CCGC 2 cut(s) 650, 653
SspMI CTAG 2 cut(s) 245, 410
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 3 cut(s) 266, 499, 514
TaiI ACGT 2 cut(s) 305, 802
TaqI TCGA 3 cut(s) 139, 444, 615
TasI AATT 6 cut(s) 275, 285, 354, 467, 558, 778
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 2 cut(s) 463, 481
Tru1I TTAA 5 cut(s) 173, 261, 434, 540, 768
Tru9I TTAA 5 cut(s) 173, 261, 434, 540, 768
TscAI CASTG 2 cut(s) 331, 404
TseFI GTSAC 2 cut(s) 322, 487
TseI GCWGC 5 cut(s) 239, 248, 455, 647, 658
Tsp45I GTSAC 2 cut(s) 322, 487
TspDTI ATGAA 4 cut(s) 19, 588, 698, 707
TspRI CASTG 2 cut(s) 331, 404
XceI RCATGY 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 138
XspI CTAG 2 cut(s) 245, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.