RchiOBHm_Chr5g0031641

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010541
Physical Location & Seq
Forward (+)
25349454 .. 25352569
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31088

Sequence Viewer

Length: 2616 bp
ATGAATCAATTTGCTCCCCTTTTTTATTTTGTTACCATACTTTTGGCATTTAGGCCAATTGCTGCAAATACAGTGAGGAATATTGAAGCTGGAATGGGCGTCGTCGTTAACGTTGGGGCCATTATAGATAACAGTTCTCGAATTGGCAAAGAACAGAGAGTGGCAATGGAAATGGCAGTGGAGGATTTTTGTGCAAATAACAGTCAGAGATTGGTTCTGCAAATAAAACACTCTCGGAGGGACCCCATGCAGGCGGCTCTTGCAGCTAGAGATCTCATAAATATGCAAGAAGTTCAAGCCATTCTAGGACCACATACATGGGAAGAGACATCATTAGTAGCTGCGGTTGGCAGTGAGAACCACATTCCAATTGTTTCTTTTGCGGAGGCTACTCCAAACTGGGCAACAGAGCTCTGGCCCTTCCTAGTTCAAGCTTCTCATAACGAGCTAAAACAAATGGAAGCAGTAGCTGCTATAGTTAAGTCTTTTGAATGGTATCAGGTCACTGTTATATACCAAGATAGAGATTATTCGTCCAACGAAATTTTGCCTGGCCTCTCTCGTGCCTTGAGACAAGTTGGCTCAGAAATAAGCCAGCTCGTAGCTCTTCCCCCTTTCGGTTCTTCTTCATTAGTCAGAGAGCTTGAGAAGCTTCGACAAGACCAATATAGAGTTGTCATCGTGCATTTGTCTTTGCCGTTGGCATTGCAACTATTTGAAAAAGCTAAAGAAATGAAGATGATGGAAAAAGACTATGTATGGATCACCACAGATCCTATTACTAGCCTAGTACATTCCTTCAATGCCTCCACCATTTCCGCAATGCAGGGGGTTATCGGAGTCAAGAGCTTCTTCCCTGAAAGTGAGAGTCCCCAATTTCAGGGTTTTCATCGTAGATTTCGTAAAAGGTTCAGCTCAGAATATCCTGAAGAAGAAAACAATGATCCTGGGGTCTTCGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCAGTGACACAAAGAAAACACAATGGGAGGCCCTTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGCTTAAGTGGAAAAATTCAGTTCACTGACCAAAAATTACCACTACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTACAGAGAACTCGGATATTGGTCAGATGGATTAGGCTTCACACAGACTATCGGGGGAAATGATACTACCAGTTCATCCATGAAAGATTTTGGATCAGTGTTTTGGCCAGGGGGGACTTTCTATACTCCAAAAGGATGGACTGTATCAACAAGTTCAAAGCCATTGAGAATCGGAGTGCCTACCGGAGCGGCATTCAAACAGTATGCAAATGTAGAAAAAGATCCCTTGGGTAATAACTTCTCATTTGGTGGAATTGCAATTGATCTCTTCGAAGAAACCCTAATCAAGCTGCCTTATCATTTGCCATATGAATTCTTCCCATTTAATGGCACATATGATTCATTAGTGCAGCAAATTCATTCTAGTAAATTTGATGCTGTAGTTGGTGATGTAGCAATAGTGTCGCAAAGATATCAGTATGCAGAATTCACAAACCCTTATTCTGAAGCAGGACTGATGATGATAGTTCCAGTCAGGTCCAAATTTTGCAGCAAAGCATGGTTATTCATGAACCCTTTCACACGAGCCATGTGGGCGCTCATAGTGGCCATCAATGTCTACAATGGTTTTGTCGTTTGGTTGATAGAGAGAAACTACTGCCCCGAACTCAGAGGTTCTCTGCCAAATCAAATTGGAACCTTGATTTGGCTTTCCTTCACCACTCTATGCTCCTTACATGAAAAGCTACATAGCAATTTGTCAAGGATGACAATGGTGGTCTGGTTATTTGTCGCACTAGTCATCACACAGACATACACCGCCAACCTCACCAGCATGCTCACGGTCCAGCAACTTGAACCAGCTGTAGCAGGTGTTGATGCACTTCTACAAAGCAATGCCGTTGTTGGTTATTGTAAGGGATCATTTGTTTCTATGTATCTAAAGAAAGTCTTGAAATTTTCCCCTGATCACATTCAGGAGTTTAGCTCCCCGGAAGAGTATGCGAAAGCCCTCAAAAGCAAAGCAATTGCTGCTGCATTTCTTGAAGTCCCTTTTGCCAAGATATTCCTCGGACAATACTGCAAAGATTTTGTGTCGGTTGGGCCAACATTCAAAGTCGGAGGATTTGGATTCGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAAATGAAGCAATGCTGAAGGTGGCCGAAAGCGGTAAGCTTCGAGAGTTGGAGAACGACATGCTGGCATCTCAGAAATGCTTGGATGCAGAAACAGATGGTAGAACCCCAAGTCTAAGCCCCAGCAGCTTTTGGGTACTATTCACATTCTCAGGAGGGACATCAACAATTGCTCTTCTGGTTTACATTTTCCGCATTCATAAATCGATGCTTAAGCACAAAACAATCTGGAACTTAATGTTGGCTGTAATCAACCAATGGGGGAATCCAAGTATGCAGAGGTTCTCAAGAAGAGACAGCGACATTAGTCCAGAAACTCCGACACATTCTGCAAATGTCCCTAACTTGCAGGCTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

871

Amino Acids

97.46

Weight (kDa)

7.99

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 36 - 364 1e-24 Periplasmic binding protein
ANF_receptor PF01094 54 - 380 1.1e-54 Receptor family ligand binding region
SBP_bac_3 PF00497 463 - 770 2.1e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 558 - 801 7.8e-24 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1946
Acc36I ACCTGC 1 cut(s) 1946
AccB7I CCANNNNNTGG 1 cut(s) 1472
AccBSI CCGCTC 1 cut(s) 1334
AccI GTMKAC 1 cut(s) 1704
AccII CGCG 1 cut(s) 959
AciI CCGC 9 cut(s) 254, 344, 383, 819, 959, 1334, 1905, 2286, 2446
AclI AACGTT 1 cut(s) 111
AclWI GGATC 6 cut(s) 767, 770, 938, 1246, 1361, 2014
AcoI YGGCCR 3 cut(s) 1250, 1692, 2277
AcsI RAATTY 8 cut(s) 543, 1077, 1457, 1500, 1514, 1571, 1628, 2042
AcuI CTGAAG 3 cut(s) 948, 1611, 2291
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 792, 2391
AfiI CCNNNNNNNGG 5 cut(s) 250, 617, 880, 1472, 1791
AflII CTTAAG 2 cut(s) 1066, 2465
AhlI ACTAGT 1 cut(s) 1882
AjnI CCWGG 3 cut(s) 550, 946, 1252
AjuI GAANNNNNNNTTGG 4 cut(s) 1774, 1806, 2477, 2509
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 3 cut(s) 320, 565, 2541
AlwI GGATC 6 cut(s) 767, 770, 938, 1246, 1361, 2014
AlwNI CAGNNNCTG 1 cut(s) 470
ApoI RAATTY 8 cut(s) 543, 1077, 1457, 1500, 1514, 1571, 1628, 2042
Asp700I GAANNNNTTC 2 cut(s) 1041, 1661
AspLEI GCGC 1 cut(s) 1684
AspS9I GGNCC 8 cut(s) 117, 241, 308, 417, 1026, 1623, 1930, 2189
AsuC2I CCSGG 1 cut(s) 2078
AsuHPI GGTGA 5 cut(s) 757, 1544, 1795, 1906, 2229
AsuII TTCGAA 2 cut(s) 1048, 1416
AvaII GGWCC 4 cut(s) 241, 308, 1623, 1930
BalI TGGCCA 2 cut(s) 1252, 1694
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 2 cut(s) 561, 1668
BbsI GAAGAC 1 cut(s) 946
Bbv12I GWGCWC 1 cut(s) 414
BccI CCATC 5 cut(s) 736, 1166, 1275, 1703, 2345
BceAI ACGGC 2 cut(s) 682, 1970
BcgI CGANNNNNNTGC 2 cut(s) 1530, 1564
BciT130I CCWGG 3 cut(s) 552, 948, 1254
BclI TGATCA 1 cut(s) 2053
BcnI CCSGG 1 cut(s) 2078
BcoDI GTCTC 3 cut(s) 320, 565, 2541
BcuI ACTAGT 1 cut(s) 1882
BfaI CTAG 9 cut(s) 267, 305, 425, 783, 788, 995, 1509, 1883, 2614
BfmI CTRYAG 3 cut(s) 474, 1524, 1950
BfoI RGCGCY 1 cut(s) 1685
BfrI CTTAAG 2 cut(s) 1066, 2465
BfuAI ACCTGC 1 cut(s) 1946
BglI GCCNNNNNGGC 1 cut(s) 1679
BglII AGATCT 1 cut(s) 271
Bme1390I CCNGG 4 cut(s) 552, 948, 1254, 2078
Bme18I GGWCC 4 cut(s) 241, 308, 1623, 1930
BmgT120I GGNCC 8 cut(s) 117, 241, 308, 417, 1026, 1623, 1930, 2189
BmiI GGNNCC 4 cut(s) 118, 242, 243, 1783
BmrFI CCNGG 4 cut(s) 552, 948, 1254, 2078
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 6 cut(s) 964, 1510, 1954, 2329, 2330, 2451
BmuI ACTGGG 1 cut(s) 409
BoxI GACNNNNGTC 1 cut(s) 2559
BpiI GAAGAC 1 cut(s) 946
BplI GAGNNNNNCTC 2 cut(s) 2057, 2089
Bpu10I CCTNAGC 1 cut(s) 2607
Bpu14I TTCGAA 2 cut(s) 1048, 1416
BpuEI CTTGAG 3 cut(s) 589, 665, 2524
BpuMI CCSGG 1 cut(s) 2078
Bsa29I ATCGAT 1 cut(s) 2459
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 6 cut(s) 947, 1253, 1371, 2076, 2155, 2228
BsaWI WCCGGW 1 cut(s) 1328
Bsc4I CCNNNNNNNGG 5 cut(s) 250, 617, 880, 1472, 1791
Bse1I ACTGG 3 cut(s) 404, 1215, 1616
Bse3DI GCAATG 5 cut(s) 171, 704, 828, 1987, 2271
BseBI CCWGG 3 cut(s) 552, 948, 1254
BseCI ATCGAT 1 cut(s) 2459
BseDI CCNNGG 6 cut(s) 947, 1253, 1371, 2076, 2155, 2228
BseGI GGATG 4 cut(s) 1220, 1286, 1857, 2344
BseLI CCNNNNNNNGG 5 cut(s) 250, 617, 880, 1472, 1791
BseMI GCAATG 5 cut(s) 171, 704, 828, 1987, 2271
BseMII CTCAG 5 cut(s) 597, 930, 1768, 2339, 2418
BseNI ACTGG 3 cut(s) 404, 1215, 1616
BseYI CCCAGC 1 cut(s) 2375
BsgI GTGCAG 1 cut(s) 1514
Bsh1236I CGCG 1 cut(s) 959
BshVI ATCGAT 1 cut(s) 2459
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 2 cut(s) 1329, 2078
BslFI GGGAC 6 cut(s) 254, 855, 1273, 2120, 2425, 2576
BslI CCNNNNNNNGG 5 cut(s) 250, 617, 880, 1472, 1791
BsmAI GTCTC 3 cut(s) 320, 565, 2541
BsmFI GGGAC 6 cut(s) 254, 855, 1273, 2120, 2425, 2576
BsmI GAATGC 2 cut(s) 1337, 2448
Bsp119I TTCGAA 2 cut(s) 1048, 1416
Bsp1286I GDGCHC 1 cut(s) 414
BspACI CCGC 9 cut(s) 254, 344, 383, 819, 959, 1334, 1905, 2286, 2446
BspCNI CTCAG 5 cut(s) 596, 929, 1767, 2338, 2417
BspDI ATCGAT 1 cut(s) 2459
BspFNI CGCG 1 cut(s) 959
BspHI TCATGA 1 cut(s) 1653
BspLI GGNNCC 4 cut(s) 118, 242, 243, 1783
BspMI ACCTGC 1 cut(s) 1946
BspPI GGATC 6 cut(s) 767, 770, 938, 1246, 1361, 2014
BspQI GCTCTTC 2 cut(s) 612, 2433
BspT104I TTCGAA 2 cut(s) 1048, 1416
BspTI CTTAAG 2 cut(s) 1066, 2465
BsrBI CCGCTC 1 cut(s) 1334
BsrDI GCAATG 5 cut(s) 171, 704, 828, 1987, 2271
BsrI ACTGG 3 cut(s) 404, 1215, 1616
BssECI CCNNGG 6 cut(s) 947, 1253, 1371, 2076, 2155, 2228
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 2 cut(s) 561, 1668
BssT1I CCWWGG 2 cut(s) 1371, 2228
Bst2BI CACGAG 2 cut(s) 561, 1668
Bst2UI CCWGG 3 cut(s) 552, 948, 1254
Bst4CI ACNGT 7 cut(s) 73, 134, 203, 508, 1288, 1347, 1930
Bst6I CTCTTC 6 cut(s) 318, 612, 1418, 2076, 2433, 2539
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 2 cut(s) 1066, 2465
BstAPI GCANNNNNTGC 2 cut(s) 470, 2117
BstBI TTCGAA 2 cut(s) 1048, 1416
BstC8I GCNNGC 5 cut(s) 252, 596, 1922, 2319, 2604
BstDEI CTNAG 7 cut(s) 583, 916, 1754, 2325, 2369, 2404, 2607
BstEII GGTNACC 1 cut(s) 2235
BstF5I GGATG 4 cut(s) 1220, 1286, 1857, 2344
BstFNI CGCG 1 cut(s) 959
BstH2I RGCGCY 1 cut(s) 1685
BstHHI GCGC 1 cut(s) 1684
BstMAI GTCTC 3 cut(s) 320, 565, 2541
BstNI CCWGG 3 cut(s) 552, 948, 1254
BstNSI RCATGY 2 cut(s) 1924, 2317
BstPAI GACNNNNGTC 1 cut(s) 2559
BstPI GGTNACC 1 cut(s) 2235
BstSCI CCNGG 4 cut(s) 550, 946, 1252, 2076
BstSFI CTRYAG 3 cut(s) 474, 1524, 1950
BstUI CGCG 1 cut(s) 959
BstV2I GAAGAC 1 cut(s) 946
BstX2I RGATCY 3 cut(s) 271, 772, 1366
BstXI CCANNNNNNTGG 3 cut(s) 43, 318, 1281
BstYI RGATCY 3 cut(s) 271, 772, 1366
Bsu15I ATCGAT 1 cut(s) 2459
BsuTUI ATCGAT 1 cut(s) 2459
BtsCI GGATG 4 cut(s) 1220, 1286, 1857, 2344
BtsI GCAGTG 4 cut(s) 183, 358, 993, 1005
BtsIMutI CAGTG 8 cut(s) 78, 183, 358, 504, 993, 1005, 1086, 1248
BveI ACCTGC 1 cut(s) 1946
Cac8I GCNNGC 5 cut(s) 252, 596, 1922, 2319, 2604
CaiI CAGNNNCTG 1 cut(s) 470
CciI TCATGA 1 cut(s) 1653
CfoI GCGC 1 cut(s) 1684
Cfr13I GGNCC 8 cut(s) 117, 241, 308, 417, 1026, 1623, 1930, 2189
ClaI ATCGAT 1 cut(s) 2459
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 791, 2390
CspCI CAANNNNNGTGG 2 cut(s) 969, 1004
CviQI GTAC 2 cut(s) 791, 2390
DdeI CTNAG 7 cut(s) 583, 916, 1754, 2325, 2369, 2404, 2607
EaeI YGGCCR 3 cut(s) 1250, 1692, 2277
Eam1104I CTCTTC 6 cut(s) 318, 612, 1418, 2076, 2433, 2539
EarI CTCTTC 6 cut(s) 318, 612, 1418, 2076, 2433, 2539
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 1371, 2228
Eco147I AGGCCT 1 cut(s) 968
Eco24I GRGCYC 1 cut(s) 414
Eco32I GATATC 1 cut(s) 1559
Eco47I GGWCC 4 cut(s) 241, 308, 1623, 1930
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 948, 1611, 2291
Eco91I GGTNACC 1 cut(s) 2235
EcoICRI GAGCTC 1 cut(s) 412
EcoO109I RGGNCCY 2 cut(s) 241, 1026
EcoO65I GGTNACC 1 cut(s) 2235
EcoRI GAATTC 2 cut(s) 1457, 1571
EcoRII CCWGG 3 cut(s) 550, 946, 1252
EcoRV GATATC 1 cut(s) 1559
EcoT14I CCWWGG 2 cut(s) 1371, 2228
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 1371, 2228
FalI AAGNNNNNCTT 4 cut(s) 836, 868, 2021, 2053
FaqI GGGAC 6 cut(s) 254, 855, 1273, 2120, 2425, 2576
FauNDI CATATG 2 cut(s) 1453, 1480
FbaI TGATCA 1 cut(s) 2053
FblI GTMKAC 1 cut(s) 1704
FokI GGATG 4 cut(s) 1207, 1293, 1864, 2351
FriOI GRGCYC 1 cut(s) 414
FspBI CTAG 9 cut(s) 267, 305, 425, 783, 788, 995, 1509, 1883, 2614
GlaI GCGC 1 cut(s) 1683
GsaI CCCAGC 1 cut(s) 2379
HaeII RGCGCY 1 cut(s) 1685
HapII CCGG 2 cut(s) 1329, 2078
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 1684
Hin1I GRCGYC 1 cut(s) 99
Hin6I GCGC 1 cut(s) 1682
HinP1I GCGC 1 cut(s) 1682
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 3 cut(s) 432, 650, 2291
HinfI GANTC 7 cut(s) 4, 840, 868, 1314, 1484, 2217, 2518
HpaI GTTAAC 1 cut(s) 109
HpaII CCGG 2 cut(s) 1329, 2078
HphI GGTGA 5 cut(s) 757, 1544, 1795, 1906, 2229
Hpy166II GTNNAC 4 cut(s) 109, 1086, 1705, 2437
Hpy8I GTNNAC 4 cut(s) 109, 1086, 1705, 2437
Hpy99I CGWCG 2 cut(s) 104, 107
HpyAV CCTTC 4 cut(s) 430, 808, 1810, 2266
HpyCH4III ACNGT 7 cut(s) 73, 134, 203, 508, 1288, 1347, 1930
HpyCH4IV ACGT 1 cut(s) 111
HpyF3I CTNAG 7 cut(s) 583, 916, 1754, 2325, 2369, 2404, 2607
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 99
HspAI GCGC 1 cut(s) 1682
KflI GGGWCCC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 2053
KspAI GTTAAC 1 cut(s) 109
LguI GCTCTTC 2 cut(s) 612, 2433
LmnI GCTCC 4 cut(s) 19, 1331, 1820, 2078
LweI GCATC 6 cut(s) 964, 1510, 1954, 2329, 2330, 2451
MaeI CTAG 9 cut(s) 267, 305, 425, 783, 788, 995, 1509, 1883, 2614
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 5 cut(s) 31, 502, 1000, 1145, 2235
MbiI CCGCTC 1 cut(s) 1334
MfeI CAATTG 5 cut(s) 57, 369, 1404, 2112, 2421
MflI RGATCY 3 cut(s) 271, 772, 1366
MhlI GDGCHC 1 cut(s) 414
MlsI TGGCCA 2 cut(s) 1252, 1694
MluNI TGGCCA 2 cut(s) 1252, 1694
MlyI GAGTC 2 cut(s) 849, 877
MmeI TCCRAC 5 cut(s) 561, 1014, 2185, 2283, 2597
Mox20I TGGCCA 2 cut(s) 1252, 1694
MroXI GAANNNNTTC 2 cut(s) 1041, 1661
MscI TGGCCA 2 cut(s) 1252, 1694
MseI TTAA 6 cut(s) 108, 480, 1067, 1470, 2466, 2489
MslI CAYNNNNRTG 3 cut(s) 281, 316, 1919
Msp20I TGGCCA 2 cut(s) 1252, 1694
MspA1I CMGCKG 1 cut(s) 1949
MspCI CTTAAG 2 cut(s) 1066, 2465
MspI CCGG 2 cut(s) 1329, 2078
MspR9I CCNGG 4 cut(s) 552, 948, 1254, 2078
MunI CAATTG 5 cut(s) 57, 369, 1404, 2112, 2421
Mva1269I GAATGC 2 cut(s) 1337, 2448
MvaI CCWGG 3 cut(s) 552, 948, 1254
MvnI CGCG 1 cut(s) 959
NciI CCSGG 1 cut(s) 2078
NdeI CATATG 2 cut(s) 1453, 1480
NlaIV GGNNCC 4 cut(s) 118, 242, 243, 1783
NmuCI GTSAC 3 cut(s) 502, 1000, 2235
NspI RCATGY 2 cut(s) 1924, 2317
NspV TTCGAA 2 cut(s) 1048, 1416
PaeI GCATGC 1 cut(s) 1924
PagI TCATGA 1 cut(s) 1653
PaqCI CACCTGC 1 cut(s) 1946
PceI AGGCCT 1 cut(s) 968
PciSI GCTCTTC 2 cut(s) 612, 2433
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 898
PctI GAATGC 2 cut(s) 1337, 2448
PdmI GAANNNNTTC 2 cut(s) 1041, 1661
PfeI GAWTC 5 cut(s) 4, 1314, 1484, 2217, 2518
PflMI CCANNNNNTGG 1 cut(s) 1472
PleI GAGTC 2 cut(s) 848, 876
PpsI GAGTC 2 cut(s) 848, 876
PpuMI RGGWCCY 1 cut(s) 241
PshAI GACNNNNGTC 1 cut(s) 2559
Psp124BI GAGCTC 1 cut(s) 414
Psp1406I AACGTT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 3 cut(s) 550, 946, 1252
PspEI GGTNACC 1 cut(s) 2235
PspFI CCCAGC 1 cut(s) 2375
PspGI CCWGG 3 cut(s) 550, 946, 1252
PspN4I GGNNCC 4 cut(s) 118, 242, 243, 1783
PspPI GGNCC 8 cut(s) 117, 241, 308, 417, 1026, 1623, 1930, 2189
PspPPI RGGWCCY 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 3 cut(s) 271, 772, 1366
PvuII CAGCTG 1 cut(s) 1949
RsaI GTAC 2 cut(s) 792, 2391
RsaNI GTAC 2 cut(s) 791, 2390
RseI CAYNNNNRTG 3 cut(s) 281, 316, 1919
SacI GAGCTC 1 cut(s) 414
SapI GCTCTTC 2 cut(s) 612, 2433
SaqAI TTAA 6 cut(s) 108, 480, 1067, 1470, 2466, 2489
Sau96I GGNCC 8 cut(s) 117, 241, 308, 417, 1026, 1623, 1930, 2189
SchI GAGTC 2 cut(s) 849, 877
ScrFI CCNGG 4 cut(s) 552, 948, 1254, 2078
SduI GDGCHC 1 cut(s) 414
SfaNI GCATC 6 cut(s) 964, 1510, 1954, 2329, 2330, 2451
SfcI CTRYAG 3 cut(s) 474, 1524, 1950
SfuI TTCGAA 2 cut(s) 1048, 1416
SinI GGWCC 4 cut(s) 241, 308, 1623, 1930
SmiMI CAYNNNNRTG 3 cut(s) 281, 316, 1919
SmlI CTYRAG 5 cut(s) 568, 644, 1066, 2465, 2539
SmoI CTYRAG 5 cut(s) 568, 644, 1066, 2465, 2539
SpeI ACTAGT 1 cut(s) 1882
SphI GCATGC 1 cut(s) 1924
SseBI AGGCCT 1 cut(s) 968
SsiI CCGC 9 cut(s) 254, 344, 383, 819, 959, 1334, 1905, 2286, 2446
SspI AATATT 2 cut(s) 82, 1042
SspMI CTAG 9 cut(s) 267, 305, 425, 783, 788, 995, 1509, 1883, 2614
SstI GAGCTC 1 cut(s) 414
StuI AGGCCT 1 cut(s) 968
StyD4I CCNGG 4 cut(s) 550, 946, 1252, 2076
StyI CCWWGG 2 cut(s) 1371, 2228
TaaI ACNGT 7 cut(s) 73, 134, 203, 508, 1288, 1347, 1930
TaiI ACGT 1 cut(s) 114
TaqI TCGA 6 cut(s) 139, 655, 1048, 1416, 2296, 2459
TatI WGTACW 1 cut(s) 790
TauI GCSGC 3 cut(s) 257, 962, 1337
TfiI GAWTC 5 cut(s) 4, 1314, 1484, 2217, 2518
Tru1I TTAA 6 cut(s) 108, 480, 1067, 1470, 2466, 2489
Tru9I TTAA 6 cut(s) 108, 480, 1067, 1470, 2466, 2489
TscAI CASTG 8 cut(s) 78, 183, 358, 511, 993, 1005, 1093, 1248
TseFI GTSAC 3 cut(s) 502, 1000, 2235
Tsp45I GTSAC 3 cut(s) 502, 1000, 2235
TspRI CASTG 8 cut(s) 78, 183, 358, 511, 993, 1005, 1093, 1248
Van91I CCANNNNNTGG 1 cut(s) 1472
Vha464I CTTAAG 2 cut(s) 1066, 2465
VpaK11BI GGWCC 4 cut(s) 241, 308, 1623, 1930
XapI RAATTY 8 cut(s) 543, 1077, 1457, 1500, 1514, 1571, 1628, 2042
XceI RCATGY 2 cut(s) 1924, 2317
XmiI GTMKAC 1 cut(s) 1704
XmnI GAANNNNTTC 2 cut(s) 1041, 1661
XspI CTAG 9 cut(s) 267, 305, 425, 783, 788, 995, 1509, 1883, 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.