Rh5BG220600

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010541
Physical Location & Seq
Forward (+)
25471402 .. 25473182
1781 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG220600.1

Sequence Viewer

Length: 561 bp
ATGCAGGGGGTTATCGGAGTCAAGAGCTTCTTCCCTGAAAGTGAGAGTCCCCAATTTCAGGGTTTTCATCGTAGATTTCGTAAAAGGTTCAGCTCAGAATATCCTGAAGAAGAAAACAATGATCCTGGGGTCTTTGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCAGTGACACAAAGGAAACACAATGGGAGGCCCTTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGTTTAAGTGGAAAAATTCAGTTCACTGACCAAAAATTACCACTACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTACAGAGAACTCGGATATTGGTCAGATGGATTAGGCTTCACACAAACTATCGGGGGAAATGATACTACCAGTTCATCCATGAAAGATTTTGGATCAGTGTTTTGGCCAGGGGGGACTTTCTATACTCCAAAAGGATGGACTGTATCAACAAGTTCAAAGCCATTGAGAATCGGAGTGCCTACCGGAGCGGCATTCAAACAGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

186

Amino Acids

20.9

Weight (kDa)

9.73

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 1 - 106 1e-08 Receptor family ligand binding region
Peripla_BP_6 PF13458 1 - 91 3.4e-06 Periplasmic binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 512
AciI CCGC 2 cut(s) 137, 512
AclWI GGATC 2 cut(s) 116, 424
AcoI YGGCCR 1 cut(s) 428
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 2 cut(s) 480, 520
AjnI CCWGG 2 cut(s) 124, 430
AluBI AGCT 2 cut(s) 27, 93
AluI AGCT 2 cut(s) 27, 93
AlwI GGATC 2 cut(s) 116, 424
AoxI GGCC 3 cut(s) 144, 203, 428
ApeKI GCWGC 1 cut(s) 155
ApoI RAATTY 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 219
AspS9I GGNCC 1 cut(s) 204
AsuHPI GGTGA 1 cut(s) 533
AsuII TTCGAA 1 cut(s) 226
BalI TGGCCA 1 cut(s) 430
BbvI GCAGC 1 cut(s) 142
BccI CCATC 2 cut(s) 344, 453
BciT130I CCWGG 2 cut(s) 126, 432
BfaI CTAG 1 cut(s) 173
BisI GCNGC 3 cut(s) 138, 156, 513
BlsI GCNGC 3 cut(s) 139, 157, 514
Bme1390I CCNGG 2 cut(s) 126, 432
BmgT120I GGNCC 1 cut(s) 204
BmrFI CCNGG 2 cut(s) 126, 432
BmsI GCATC 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 125, 431, 532
BsaWI WCCGGW 1 cut(s) 506
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 393
BseBI CCWGG 2 cut(s) 126, 432
BseDI CCNNGG 3 cut(s) 125, 431, 532
BseGI GGATG 2 cut(s) 398, 464
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 142
BshFI GGCC 3 cut(s) 146, 205, 430
BsiSI CCGG 1 cut(s) 507
BslFI GGGAC 2 cut(s) 33, 451
BslI CCNNNNNNNGG 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 33, 451
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 3 cut(s) 146, 205, 430
Bsp119I TTCGAA 1 cut(s) 226
Bsp143I GATC 3 cut(s) 121, 300, 416
BspACI CCGC 2 cut(s) 137, 512
BspANI GGCC 3 cut(s) 146, 205, 430
BspCNI CTCAG 1 cut(s) 107
BspPI GGATC 2 cut(s) 116, 424
BspT104I TTCGAA 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 512
BsrI ACTGG 1 cut(s) 393
BssECI CCNNGG 3 cut(s) 125, 431, 532
BssMI GATC 3 cut(s) 121, 300, 416
BssT1I CCWWGG 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 126, 432
Bst4CI ACNGT 1 cut(s) 466
BstBI TTCGAA 1 cut(s) 226
BstDEI CTNAG 1 cut(s) 94
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 2 cut(s) 398, 464
BstKTI GATC 3 cut(s) 124, 303, 419
BstMBI GATC 3 cut(s) 121, 300, 416
BstMWI GCNNNNNNNGC 3 cut(s) 143, 152, 161
BstNI CCWGG 2 cut(s) 126, 432
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 124, 430
BstV1I GCAGC 1 cut(s) 142
BstXI CCANNNNNNTGG 1 cut(s) 459
BsuRI GGCC 3 cut(s) 146, 205, 430
BtsCI GGATG 2 cut(s) 398, 464
BtsI GCAGTG 2 cut(s) 171, 183
BtsIMutI CAGTG 4 cut(s) 171, 183, 264, 426
Cfr13I GGNCC 1 cut(s) 204
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 2 cut(s) 239, 403
CviJI RGCY 7 cut(s) 27, 93, 146, 205, 360, 430, 484
CviKI_1 RGCY 7 cut(s) 27, 93, 146, 205, 360, 430, 484
DdeI CTNAG 1 cut(s) 94
DpnI GATC 3 cut(s) 123, 302, 418
DpnII GATC 3 cut(s) 121, 300, 416
EaeI YGGCCR 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 532
Eco147I AGGCCT 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 126
Eco91I GGTNACC 1 cut(s) 539
EcoO109I RGGNCCY 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 539
EcoRII CCWGG 2 cut(s) 124, 430
EcoT14I CCWWGG 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 2 cut(s) 242, 406
FaiI YATR 5 cut(s) 150, 240, 314, 404, 447
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 2 cut(s) 33, 451
FatI CATG 2 cut(s) 238, 402
Fnu4HI GCNGC 3 cut(s) 138, 156, 513
FokI GGATG 2 cut(s) 385, 471
Fsp4HI GCNGC 3 cut(s) 138, 156, 513
FspBI CTAG 1 cut(s) 173
GluI GCNGC 3 cut(s) 138, 156, 513
HaeIII GGCC 3 cut(s) 146, 205, 430
HapII CCGG 1 cut(s) 507
Hin1II CATG 2 cut(s) 242, 406
HinfI GANTC 3 cut(s) 18, 46, 492
HpaII CCGG 1 cut(s) 507
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 6 cut(s) 17, 97, 338, 349, 497, 554
Hpy188III TCNNGA 3 cut(s) 22, 104, 298
Hpy8I GTNNAC 1 cut(s) 264
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 2 cut(s) 4, 158
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 152, 161
HpyF3I CTNAG 1 cut(s) 94
Hsp92II CATG 2 cut(s) 242, 406
Kzo9I GATC 3 cut(s) 121, 300, 416
LmnI GCTCC 1 cut(s) 509
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 173
MaeIII GTNAC 3 cut(s) 178, 323, 539
MalI GATC 3 cut(s) 123, 302, 418
MbiI CCGCTC 1 cut(s) 512
MboI GATC 3 cut(s) 121, 300, 416
MboII GAAGA 4 cut(s) 22, 119, 122, 215
MlsI TGGCCA 1 cut(s) 430
MluCI AATT 3 cut(s) 53, 255, 275
MluNI TGGCCA 1 cut(s) 430
MlyI GAGTC 2 cut(s) 27, 55
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 219
MscI TGGCCA 1 cut(s) 430
MseI TTAA 1 cut(s) 245
Msp20I TGGCCA 1 cut(s) 430
MspI CCGG 1 cut(s) 507
MspR9I CCNGG 2 cut(s) 126, 432
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 2 cut(s) 126, 432
MwoI GCNNNNNNNGC 3 cut(s) 143, 152, 161
NdeII GATC 3 cut(s) 121, 300, 416
NlaIII CATG 2 cut(s) 242, 406
NmuCI GTSAC 2 cut(s) 178, 539
NspV TTCGAA 1 cut(s) 226
PceI AGGCCT 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 76
PctI GAATGC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 492
PkrI GCNGC 3 cut(s) 139, 157, 514
PleI GAGTC 2 cut(s) 26, 54
PpsI GAGTC 2 cut(s) 26, 54
Psp6I CCWGG 2 cut(s) 124, 430
PspEI GGTNACC 1 cut(s) 539
PspGI CCWGG 2 cut(s) 124, 430
PspPI GGNCC 1 cut(s) 204
SaqAI TTAA 1 cut(s) 245
SatI GCNGC 3 cut(s) 138, 156, 513
Sau3AI GATC 3 cut(s) 121, 300, 416
Sau96I GGNCC 1 cut(s) 204
SchI GAGTC 2 cut(s) 27, 55
ScrFI CCNGG 2 cut(s) 126, 432
SetI ASST 3 cut(s) 29, 89, 95
SfaNI GCATC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 226
Sse9I AATT 3 cut(s) 53, 255, 275
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 2 cut(s) 137, 512
SspI AATATT 1 cut(s) 220
SspMI CTAG 1 cut(s) 173
StuI AGGCCT 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 124, 430
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 1 cut(s) 466
TaqI TCGA 1 cut(s) 226
TasI AATT 3 cut(s) 53, 255, 275
TauI GCSGC 2 cut(s) 140, 515
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 1 cut(s) 245
Tru9I TTAA 1 cut(s) 245
TscAI CASTG 4 cut(s) 171, 183, 271, 426
TseFI GTSAC 2 cut(s) 178, 539
TseI GCWGC 1 cut(s) 155
Tsp45I GTSAC 2 cut(s) 178, 539
TspDTI ATGAA 4 cut(s) 56, 227, 387, 419
TspRI CASTG 4 cut(s) 171, 183, 271, 426
XapI RAATTY 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.