Rh5CG244100

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010541
Physical Location & Seq
Forward (+)
25425672 .. 25428383
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG244100.1

Sequence Viewer

Length: 2337 bp
ATGCAAGAAGTTCAAGCCATTCTAGGACCACATACATGGGAAGAGACATCATTAGTAGCTGCGGTTGGCAGTGAGAACCACATTCCAATTGTTTCTTTTGCGGAGGCTACTCCAAACTGGGCAACAGAGCTCTGGCCCTTCCTAGTTCAAGCTTCTCATAACGAGCTAAAACAAATGGAAGCAGTAGCTGCTATAGTTAAGTCTTTTGAATGGTATCAGGTCACTGTTATATACCAAGATAGAGATTATTCGTCCAACGAAATTTTGCCTGGCCTCTCTCGTGCCTTGAGACAAGTTGGCTCAGAAATAAGCCAGCTCGTAGCTCTTCCCCCTTTCGGTTCTTCTTCATTAGTCAGAGAGCTTGAGAAGCTTCGACAAGACCAATATAGAGTTGTCATCGTGCATTTGTCTTTGCCGTTGGCATTGCAACTATTTGAAAAAGCTAAAGAAATGAAGATGATGGAAAAAGACTATGTATGGATCACCACAGATCCTATTACTAGCCTAGTACATTCCTTCAATGCCTCCACCATTTCCGCAATGCAGGGGGTTATCGGAGTCAAGAGCTTCTTCCCTGAAAGTGAGAGTCCCCAATTTCAGGGTTTTCATCGTAGATTTCGTAAAAGGTTCAGCTCAGAATATCCTGAAGAAGAAAACAATGATCCTGGGGTCTTCGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCAGTGACACAAAGAAAACACAATGGGAGGCCCTTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGCCTAAGTGGAAAAATTCAGTTCACTGACCAAAAATTACCACTACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTACAGAGAACTCGGATATTGGTCAGATGGATTAGGCTTCACACAGACTATCGGGGGAAATGATACTACCAGTTCATCCATGAAAGATTTTGGATCAGTGTTTTGGCCAGGGGGGACTTTCCATACTCCAAAAGGATGGACTGTATCAACAAGTTCAAAGCCATTGAGAATCGGAGTGCCTACCGGAGCGGCATTCAAACAGTATGCAAATGTAGAAAAAGATCCCTTGGGTAATAACTTCTCATTTGGTGGAATTGCAATTGATCTCTTCGAAGAAACCCTAATCAAGCTGCCTTATCATTTGCCATATGAATTCTTCCCATTTAATGGCACATATGATTCATTAGTGCAGCAAATTCATTCTAGTAAATTTGATGCTGTAGTTGGTGATGTAGCAATAGTGTCGCAAAGATATCAGTATGCAGAATTCACAAACCCTTATTCTGAAGCAGGACTGATGATGATAGTTCCAGTCAGGTCCAAATTTTGCAGCAAAGCATGGTTATTCATGAACCCTTTCACACGAGCCATGTGGGCGCTCATAGTGGCCATCAATGTCTACAATGGTTTTGTCGTTTGGTTGATAGAGAGAAACTACTGCCCCGAACTCAGAGGTTCTCTGCCAAATCAAATTGGAACCTTGATTTGGCTTTCCTTCACCACTCTATGCTCCTTACATGGAGAAAAGCTACATAGCAATTTGTCAAGGATGACAATGGTGGTCTGGTTATTTGTCGCACTAGTCATCACACAGACATACACCGCCAACCTCACCAGCATGCTCACGGTCCAGCAACTTGAACCAGCTGTAGCAGGTGTTGATGCACTTCTACAAAGCAATGCCGTTGTTGGTTATTGTAAGGGATCATTTGTTTCTATGTATCTAAAGAAAGTCTTGAAATTTTCCCCTGATCACATTCAGGAGTTTAGCTCCCCGGAAGAGTATGCGAAAGCCCTCAAAAGCAAAGCAATTGCTGCTGCATTTCTTGAAGTCCCTTTTGCCAAGATATTCCTCGGACAATACTGCAAAGATTTTGTGTCGGTTGGGCCAACATTCAAAGTCGGAGGATTTGGATTCGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAAATGAAGCAATGCTGAAGGTGGCCGAAAGCGGTAAGCTTCGAGACTTGGAGAACGACATGCTGGCATCTCAGAAATGCTTGGATGCAGAAACAGATGGTAGAACCCCAAGTCTAAGCCCCAGCAGCTTTTGGGTACTATTCACATTCTCAGGAGGGACATCAACAATTGCTCTTCTGGTTTACATTTTCCGCATTTATAAATCGATGCTTAAGCACAAAACAATCTGGAACTTAATGTTGGCTGTAATCAACCAATGGGGGAATCCAAGTATGCAGAGGTTCTCAAGAAGAGACAGCGACATTAGTCCAGAAACTCCGACACATTCTGCAAATGTCCCTAACTTGCAGGCTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

778

Amino Acids

87.09

Weight (kDa)

7.26

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 2 - 286 3.3e-47 Receptor family ligand binding region
Peripla_BP_6 PF13458 2 - 270 5.6e-17 Periplasmic binding protein
SBP_bac_3 PF00497 369 - 677 4.1e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 464 - 708 6.9e-25 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2175
AarI CACCTGC 1 cut(s) 1667
Acc36I ACCTGC 1 cut(s) 1667
AccB7I CCANNNNNTGG 1 cut(s) 1190
AccBSI CCGCTC 1 cut(s) 1052
AccI GTMKAC 1 cut(s) 1422
AccII CGCG 1 cut(s) 677
AciI CCGC 8 cut(s) 62, 101, 537, 677, 1052, 1626, 2007, 2167
AclWI GGATC 6 cut(s) 485, 488, 656, 964, 1079, 1735
AcoI YGGCCR 3 cut(s) 968, 1410, 1998
AcsI RAATTY 8 cut(s) 261, 795, 1175, 1218, 1232, 1289, 1346, 1763
AcuI CTGAAG 3 cut(s) 666, 1329, 2012
AfaI GTAC 2 cut(s) 510, 2112
AfiI CCNNNNNNNGG 4 cut(s) 335, 598, 1190, 1509
AflII CTTAAG 1 cut(s) 2186
AhlI ACTAGT 1 cut(s) 1603
AjnI CCWGG 3 cut(s) 268, 664, 970
AjuI GAANNNNNNNTTGG 4 cut(s) 1492, 1524, 2198, 2230
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 4 cut(s) 38, 283, 2013, 2262
AlwI GGATC 6 cut(s) 485, 488, 656, 964, 1079, 1735
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 9 cut(s) 134, 271, 684, 743, 781, 968, 1410, 1910, 1998
ApeKI GCWGC 9 cut(s) 59, 188, 695, 1153, 1213, 1353, 1838, 1841, 2100
ApoI RAATTY 8 cut(s) 261, 795, 1175, 1218, 1232, 1289, 1346, 1763
Asp700I GAANNNNTTC 2 cut(s) 759, 1379
AspLEI GCGC 1 cut(s) 1402
AspS9I GGNCC 6 cut(s) 26, 135, 744, 1341, 1651, 1910
AsuC2I CCSGG 1 cut(s) 1799
AsuHPI GGTGA 5 cut(s) 475, 1262, 1513, 1627, 1950
AsuII TTCGAA 2 cut(s) 766, 1134
AvaII GGWCC 3 cut(s) 26, 1341, 1651
BalI TGGCCA 2 cut(s) 970, 1412
BanII GRGCYC 1 cut(s) 132
BauI CACGAG 2 cut(s) 279, 1386
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 9 cut(s) 46, 175, 682, 1140, 1225, 1365, 1825, 1828, 2112
BccI CCATC 5 cut(s) 454, 884, 993, 1421, 2066
BceAI ACGGC 2 cut(s) 400, 1691
BcgI CGANNNNNNTGC 2 cut(s) 1248, 1282
BciT130I CCWGG 3 cut(s) 270, 666, 972
BclI TGATCA 1 cut(s) 1774
BcnI CCSGG 1 cut(s) 1799
BcoDI GTCTC 4 cut(s) 38, 283, 2013, 2262
BcuI ACTAGT 1 cut(s) 1603
BfaI CTAG 8 cut(s) 23, 143, 501, 506, 713, 1227, 1604, 2335
BfmI CTRYAG 3 cut(s) 192, 1242, 1671
BfoI RGCGCY 1 cut(s) 1403
BfrI CTTAAG 1 cut(s) 2186
BfuAI ACCTGC 1 cut(s) 1667
BglI GCCNNNNNGGC 1 cut(s) 1397
Bme1390I CCNGG 4 cut(s) 270, 666, 972, 1799
Bme18I GGWCC 3 cut(s) 26, 1341, 1651
BmgT120I GGNCC 6 cut(s) 26, 135, 744, 1341, 1651, 1910
BmiI GGNNCC 1 cut(s) 1501
BmrFI CCNGG 4 cut(s) 270, 666, 972, 1799
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 6 cut(s) 682, 1228, 1675, 2050, 2051, 2172
BmuI ACTGGG 1 cut(s) 127
BoxI GACNNNNGTC 1 cut(s) 2280
BpiI GAAGAC 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 1778, 1810
Bpu10I CCTNAGC 1 cut(s) 2328
Bpu14I TTCGAA 2 cut(s) 766, 1134
BpuEI CTTGAG 3 cut(s) 307, 383, 2245
BpuMI CCSGG 1 cut(s) 1799
Bsa29I ATCGAT 1 cut(s) 2180
BsaJI CCNNGG 6 cut(s) 665, 971, 1089, 1797, 1876, 1949
BsaWI WCCGGW 1 cut(s) 1046
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 598, 1190, 1509
Bse1I ACTGG 3 cut(s) 122, 933, 1334
Bse3DI GCAATG 4 cut(s) 422, 546, 1708, 1992
BseBI CCWGG 3 cut(s) 270, 666, 972
BseCI ATCGAT 1 cut(s) 2180
BseDI CCNNGG 6 cut(s) 665, 971, 1089, 1797, 1876, 1949
BseGI GGATG 4 cut(s) 938, 1004, 1578, 2065
BseLI CCNNNNNNNGG 4 cut(s) 335, 598, 1190, 1509
BseMI GCAATG 4 cut(s) 422, 546, 1708, 1992
BseMII CTCAG 5 cut(s) 315, 648, 1486, 2060, 2139
BseNI ACTGG 3 cut(s) 122, 933, 1334
BseXI GCAGC 9 cut(s) 46, 175, 682, 1140, 1225, 1365, 1825, 1828, 2112
BseYI CCCAGC 1 cut(s) 2096
BsgI GTGCAG 1 cut(s) 1232
Bsh1236I CGCG 1 cut(s) 677
BshFI GGCC 9 cut(s) 136, 273, 686, 745, 783, 970, 1412, 1912, 2000
BshVI ATCGAT 1 cut(s) 2180
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 2 cut(s) 1047, 1799
BslFI GGGAC 5 cut(s) 573, 991, 1841, 2146, 2297
BslI CCNNNNNNNGG 4 cut(s) 335, 598, 1190, 1509
BsmAI GTCTC 4 cut(s) 38, 283, 2013, 2262
BsmFI GGGAC 5 cut(s) 573, 991, 1841, 2146, 2297
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 9 cut(s) 136, 273, 686, 745, 783, 970, 1412, 1912, 2000
Bsp119I TTCGAA 2 cut(s) 766, 1134
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
BspACI CCGC 8 cut(s) 62, 101, 537, 677, 1052, 1626, 2007, 2167
BspANI GGCC 9 cut(s) 136, 273, 686, 745, 783, 970, 1412, 1912, 2000
BspCNI CTCAG 5 cut(s) 314, 647, 1485, 2059, 2138
BspDI ATCGAT 1 cut(s) 2180
BspFNI CGCG 1 cut(s) 677
BspHI TCATGA 1 cut(s) 1371
BspLI GGNNCC 1 cut(s) 1501
BspMI ACCTGC 1 cut(s) 1667
BspPI GGATC 6 cut(s) 485, 488, 656, 964, 1079, 1735
BspQI GCTCTTC 2 cut(s) 330, 2154
BspT104I TTCGAA 2 cut(s) 766, 1134
BspTI CTTAAG 1 cut(s) 2186
BsrBI CCGCTC 1 cut(s) 1052
BsrDI GCAATG 4 cut(s) 422, 546, 1708, 1992
BsrI ACTGG 3 cut(s) 122, 933, 1334
BssECI CCNNGG 6 cut(s) 665, 971, 1089, 1797, 1876, 1949
BssMI GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
BssSI CACGAG 2 cut(s) 279, 1386
BssT1I CCWWGG 2 cut(s) 1089, 1949
Bst2BI CACGAG 2 cut(s) 279, 1386
Bst2UI CCWGG 3 cut(s) 270, 666, 972
Bst4CI ACNGT 4 cut(s) 226, 1006, 1065, 1651
Bst6I CTCTTC 6 cut(s) 36, 330, 1136, 1797, 2154, 2260
BstAFI CTTAAG 1 cut(s) 2186
BstAPI GCANNNNNTGC 2 cut(s) 188, 1838
BstBI TTCGAA 2 cut(s) 766, 1134
BstC8I GCNNGC 4 cut(s) 314, 1643, 2040, 2325
BstDEI CTNAG 8 cut(s) 301, 634, 785, 1472, 2046, 2090, 2125, 2328
BstEII GGTNACC 1 cut(s) 1956
BstF5I GGATG 4 cut(s) 938, 1004, 1578, 2065
BstFNI CGCG 1 cut(s) 677
BstH2I RGCGCY 1 cut(s) 1403
BstHHI GCGC 1 cut(s) 1402
BstKTI GATC 9 cut(s) 483, 493, 664, 843, 959, 1087, 1129, 1730, 1777
BstMAI GTCTC 4 cut(s) 38, 283, 2013, 2262
BstMBI GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
BstMWI GCNNNNNNNGC 8 cut(s) 188, 367, 683, 692, 701, 1397, 1838, 2100
BstNI CCWGG 3 cut(s) 270, 666, 972
BstNSI RCATGY 2 cut(s) 1645, 2038
BstPAI GACNNNNGTC 1 cut(s) 2280
BstPI GGTNACC 1 cut(s) 1956
BstSCI CCNGG 4 cut(s) 268, 664, 970, 1797
BstSFI CTRYAG 3 cut(s) 192, 1242, 1671
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 9 cut(s) 46, 175, 682, 1140, 1225, 1365, 1825, 1828, 2112
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 490, 1084
BstXI CCANNNNNNTGG 2 cut(s) 36, 999
BstYI RGATCY 2 cut(s) 490, 1084
Bsu15I ATCGAT 1 cut(s) 2180
BsuRI GGCC 9 cut(s) 136, 273, 686, 745, 783, 970, 1412, 1912, 2000
BsuTUI ATCGAT 1 cut(s) 2180
BtsCI GGATG 4 cut(s) 938, 1004, 1578, 2065
BtsI GCAGTG 3 cut(s) 76, 711, 723
BtsIMutI CAGTG 6 cut(s) 76, 222, 711, 723, 804, 966
BveI ACCTGC 1 cut(s) 1667
Cac8I GCNNGC 4 cut(s) 314, 1643, 2040, 2325
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 1371
CfoI GCGC 1 cut(s) 1402
Cfr13I GGNCC 6 cut(s) 26, 135, 744, 1341, 1651, 1910
ClaI ATCGAT 1 cut(s) 2180
Csp6I GTAC 2 cut(s) 509, 2111
CspCI CAANNNNNGTGG 2 cut(s) 687, 722
CviAII CATG 9 cut(s) 36, 779, 943, 1362, 1372, 1393, 1541, 1642, 2035
CviQI GTAC 2 cut(s) 509, 2111
DdeI CTNAG 8 cut(s) 301, 634, 785, 1472, 2046, 2090, 2125, 2328
DpnI GATC 9 cut(s) 482, 492, 663, 842, 958, 1086, 1128, 1729, 1776
DpnII GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
EaeI YGGCCR 3 cut(s) 968, 1410, 1998
Eam1104I CTCTTC 6 cut(s) 36, 330, 1136, 1797, 2154, 2260
EarI CTCTTC 6 cut(s) 36, 330, 1136, 1797, 2154, 2260
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 1089, 1949
Eco147I AGGCCT 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 1277
Eco47I GGWCC 3 cut(s) 26, 1341, 1651
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 3 cut(s) 666, 1329, 2012
Eco91I GGTNACC 1 cut(s) 1956
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 744
EcoO65I GGTNACC 1 cut(s) 1956
EcoRI GAATTC 2 cut(s) 1175, 1289
EcoRII CCWGG 3 cut(s) 268, 664, 970
EcoRV GATATC 1 cut(s) 1277
EcoT14I CCWWGG 2 cut(s) 1089, 1949
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 1089, 1949
FaeI CATG 9 cut(s) 39, 782, 946, 1365, 1375, 1396, 1544, 1645, 2038
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 1742, 1774
FaqI GGGAC 5 cut(s) 573, 991, 1841, 2146, 2297
FatI CATG 9 cut(s) 35, 778, 942, 1361, 1371, 1392, 1540, 1641, 2034
FauNDI CATATG 2 cut(s) 1171, 1198
FbaI TGATCA 1 cut(s) 1774
FblI GTMKAC 1 cut(s) 1422
FokI GGATG 4 cut(s) 925, 1011, 1585, 2072
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 8 cut(s) 23, 143, 501, 506, 713, 1227, 1604, 2335
GlaI GCGC 1 cut(s) 1401
GsaI CCCAGC 1 cut(s) 2100
HaeII RGCGCY 1 cut(s) 1403
HaeIII GGCC 9 cut(s) 136, 273, 686, 745, 783, 970, 1412, 1912, 2000
HapII CCGG 2 cut(s) 1047, 1799
HhaI GCGC 1 cut(s) 1402
Hin1II CATG 9 cut(s) 39, 782, 946, 1365, 1375, 1396, 1544, 1645, 2038
Hin6I GCGC 1 cut(s) 1400
HinP1I GCGC 1 cut(s) 1400
HindIII AAGCTT 3 cut(s) 150, 368, 2012
HinfI GANTC 6 cut(s) 558, 586, 1032, 1202, 1938, 2239
HpaII CCGG 2 cut(s) 1047, 1799
HphI GGTGA 5 cut(s) 475, 1262, 1513, 1627, 1950
Hpy166II GTNNAC 3 cut(s) 804, 1423, 2158
Hpy8I GTNNAC 3 cut(s) 804, 1423, 2158
HpyAV CCTTC 4 cut(s) 148, 526, 1528, 1987
HpyCH4III ACNGT 4 cut(s) 226, 1006, 1065, 1651
HpyF10VI GCNNNNNNNGC 8 cut(s) 188, 367, 683, 692, 701, 1397, 1838, 2100
HpyF3I CTNAG 8 cut(s) 301, 634, 785, 1472, 2046, 2090, 2125, 2328
Hsp92II CATG 9 cut(s) 39, 782, 946, 1365, 1375, 1396, 1544, 1645, 2038
HspAI GCGC 1 cut(s) 1400
Ksp22I TGATCA 1 cut(s) 1774
Kzo9I GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
LguI GCTCTTC 2 cut(s) 330, 2154
LmnI GCTCC 3 cut(s) 1049, 1538, 1799
Lsp1109I GCAGC 9 cut(s) 46, 175, 682, 1140, 1225, 1365, 1825, 1828, 2112
LweI GCATC 6 cut(s) 682, 1228, 1675, 2050, 2051, 2172
MaeI CTAG 8 cut(s) 23, 143, 501, 506, 713, 1227, 1604, 2335
MaeIII GTNAC 4 cut(s) 220, 718, 863, 1956
MalI GATC 9 cut(s) 482, 492, 663, 842, 958, 1086, 1128, 1729, 1776
MbiI CCGCTC 1 cut(s) 1052
MboI GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
MfeI CAATTG 4 cut(s) 87, 1122, 1833, 2142
MflI RGATCY 2 cut(s) 490, 1084
MhlI GDGCHC 1 cut(s) 132
MlsI TGGCCA 2 cut(s) 970, 1412
MluNI TGGCCA 2 cut(s) 970, 1412
MlyI GAGTC 2 cut(s) 567, 595
MmeI TCCRAC 4 cut(s) 279, 732, 1906, 2318
Mox20I TGGCCA 2 cut(s) 970, 1412
MroXI GAANNNNTTC 2 cut(s) 759, 1379
MscI TGGCCA 2 cut(s) 970, 1412
MseI TTAA 4 cut(s) 198, 1188, 2187, 2210
MslI CAYNNNNRTG 2 cut(s) 34, 1640
Msp20I TGGCCA 2 cut(s) 970, 1412
MspA1I CMGCKG 1 cut(s) 1670
MspCI CTTAAG 1 cut(s) 2186
MspI CCGG 2 cut(s) 1047, 1799
MspR9I CCNGG 4 cut(s) 270, 666, 972, 1799
MunI CAATTG 4 cut(s) 87, 1122, 1833, 2142
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 3 cut(s) 270, 666, 972
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 8 cut(s) 188, 367, 683, 692, 701, 1397, 1838, 2100
NciI CCSGG 1 cut(s) 1799
NdeI CATATG 2 cut(s) 1171, 1198
NdeII GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
NlaIII CATG 9 cut(s) 39, 782, 946, 1365, 1375, 1396, 1544, 1645, 2038
NlaIV GGNNCC 1 cut(s) 1501
NmuCI GTSAC 3 cut(s) 220, 718, 1956
NspI RCATGY 2 cut(s) 1645, 2038
NspV TTCGAA 2 cut(s) 766, 1134
PaeI GCATGC 1 cut(s) 1645
PagI TCATGA 1 cut(s) 1371
PaqCI CACCTGC 1 cut(s) 1667
PceI AGGCCT 1 cut(s) 686
PciSI GCTCTTC 2 cut(s) 330, 2154
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 1 cut(s) 1055
PdmI GAANNNNTTC 2 cut(s) 759, 1379
PfeI GAWTC 4 cut(s) 1032, 1202, 1938, 2239
PflMI CCANNNNNTGG 1 cut(s) 1190
PleI GAGTC 2 cut(s) 566, 594
PpsI GAGTC 2 cut(s) 566, 594
PshAI GACNNNNGTC 1 cut(s) 2280
PsiI TTATAA 1 cut(s) 2175
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 268, 664, 970
PspEI GGTNACC 1 cut(s) 1956
PspFI CCCAGC 1 cut(s) 2096
PspGI CCWGG 3 cut(s) 268, 664, 970
PspN4I GGNNCC 1 cut(s) 1501
PspPI GGNCC 6 cut(s) 26, 135, 744, 1341, 1651, 1910
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 490, 1084
PvuII CAGCTG 1 cut(s) 1670
RsaI GTAC 2 cut(s) 510, 2112
RsaNI GTAC 2 cut(s) 509, 2111
RseI CAYNNNNRTG 2 cut(s) 34, 1640
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 2 cut(s) 330, 2154
SaqAI TTAA 4 cut(s) 198, 1188, 2187, 2210
Sau3AI GATC 9 cut(s) 480, 490, 661, 840, 956, 1084, 1126, 1727, 1774
Sau96I GGNCC 6 cut(s) 26, 135, 744, 1341, 1651, 1910
SchI GAGTC 2 cut(s) 567, 595
ScrFI CCNGG 4 cut(s) 270, 666, 972, 1799
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 6 cut(s) 682, 1228, 1675, 2050, 2051, 2172
SfcI CTRYAG 3 cut(s) 192, 1242, 1671
SfuI TTCGAA 2 cut(s) 766, 1134
SinI GGWCC 3 cut(s) 26, 1341, 1651
SmiMI CAYNNNNRTG 2 cut(s) 34, 1640
SmlI CTYRAG 4 cut(s) 286, 362, 2186, 2260
SmoI CTYRAG 4 cut(s) 286, 362, 2186, 2260
SpeI ACTAGT 1 cut(s) 1603
SphI GCATGC 1 cut(s) 1645
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 8 cut(s) 62, 101, 537, 677, 1052, 1626, 2007, 2167
SspI AATATT 1 cut(s) 760
SspMI CTAG 8 cut(s) 23, 143, 501, 506, 713, 1227, 1604, 2335
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 4 cut(s) 268, 664, 970, 1797
StyI CCWWGG 2 cut(s) 1089, 1949
TaaI ACNGT 4 cut(s) 226, 1006, 1065, 1651
TaqI TCGA 5 cut(s) 373, 766, 1134, 2017, 2180
TatI WGTACW 1 cut(s) 508
TauI GCSGC 2 cut(s) 680, 1055
TfiI GAWTC 4 cut(s) 1032, 1202, 1938, 2239
Tru1I TTAA 4 cut(s) 198, 1188, 2187, 2210
Tru9I TTAA 4 cut(s) 198, 1188, 2187, 2210
TscAI CASTG 6 cut(s) 76, 229, 711, 723, 811, 966
TseFI GTSAC 3 cut(s) 220, 718, 1956
TseI GCWGC 9 cut(s) 59, 188, 695, 1153, 1213, 1353, 1838, 1841, 2100
Tsp45I GTSAC 3 cut(s) 220, 718, 1956
TspRI CASTG 6 cut(s) 76, 229, 711, 723, 811, 966
Van91I CCANNNNNTGG 1 cut(s) 1190
Vha464I CTTAAG 1 cut(s) 2186
VpaK11BI GGWCC 3 cut(s) 26, 1341, 1651
XapI RAATTY 8 cut(s) 261, 795, 1175, 1218, 1232, 1289, 1346, 1763
XceI RCATGY 2 cut(s) 1645, 2038
XmiI GTMKAC 1 cut(s) 1422
XmnI GAANNNNTTC 2 cut(s) 759, 1379
XspI CTAG 8 cut(s) 23, 143, 501, 506, 713, 1227, 1604, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.