MD03G1237800.v1.1

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010541
Physical Location & Seq
Reverse (-)
32290456 .. 32294292
3837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1237800.v1.1.491

Sequence Viewer

Length: 2616 bp
ATGTCTCAGTTTGCTCTTCTTTTTTATTTCCTGATCACAGTTTCAGTTAGGGAAGGAATTACAACATTTGGAAATGCCAATACAATAGAGAACGAACCCGGAATGGGACTAGGTGCTATTATCGACAACAGTTCTCGGATTGGAAAAGAAGAGAGAGTGGCAATTCAAATGGCATTGGATGATCATTTTGATTCCACTGGGAATCAGAGAATGGTTCTGCACGTAAGAAACTCTCAGAGGGAGCCCTTGCAGGCAGCTCTTGCAGCTAAATTTCTTATAGATGAGCAAAAAGTGCTAGCTATTATAGGACCTCATACATGGGAAGAGACATCATTAGTAGCTAAGGTTGGCAGTGAGACTCATACCGCAATTGTTTCCCTTGCTGAGGCTAATCCAATGTGGGCAACTGAGCTGTGGCCCTTTCTGGTTCAAGCTTCTCGTAACCGGTTGAAACAAATGGAAGCAATTGCTGCTATAGTTCAGTCTTGGGAATGGCATCAAGTCACTGTAATTTACCAAGATAGAGATTCTTCAGCCAATGAAGTCTTACCTCATCTCTCTGATGCCTTGAGACAAGTTGGTGCAGAAATTAGCCACCTTGTAGCTCTCCCTCCTTTTGCTTCTTCTTCATTAATTGAAGAGCTTGAGAAAATCCGAAGAGACCAGTGTAGAGTCTTTGTTGTACATTTGTCTGCGCAACTGGCAGTGCAACTATTCCGGAAGGCAAAAGAAATGGAGATGATGGAAAAGGATTATGTTTGGATCACCACTGATCCTATTACAAGCCTTGTCCACTCCTTTAATGCCTCCACCATCTCAACAATGCAAGGGATCATCGGACTCAAGAGCTACCTCCCCGAAAGCGGGAAACAATCTAAGGACTTTGATCATAAGTTTCGTAAAAGGTTTAGCTCGGAACACCCTGAAGAGGAGAACAATGAACCTGGAATCTTTGCAGCACAGGCTTATGATGCTGCAAGGGCAGTGGCTTTAGCTGTGACAGAAAGCAGGAAAGGGAGGAACTTGTTAGCAAAACTTTTGCAAAGCGATTTTCATGGCTTAAATGGAAGAATTAAGTTCAGTGACCAAAAATTAGCTCCACAACATGTATTTCAAATCATCAATGTGAATGGAAAGAGTTACAGAGAAGTTGGATACTGGTCAGATGGATTAGGATTCTCACTGACTCTAGGTGAACGTGCTACTAACAGATCTTCTATGAAATATTTGGGGCAAGTGTTTTGGCCAGGGGGGCCTTGGTTGACTCCAAAAGGGTGGACTGAACCAACAACAAACGCAAACGTGCTGAGAATTGGCGTGCCGACAGGATCAACGTTTAAACAGTACGTAAATGTGAAGAAAGATCCATTGGGACATAACTTATCTTTCAATGGACTTGCAATAGATCTCTTCAAAGCAACCCTAAAGGAGCTGCCTTACCATTTGCCCTACGAGTTCTTTCCCTTCGAAGGAACATACGATTCTTTAGTTGAGCAGATCCATTTGCATAAATTTGATGCTGTAGTTGGTGATGTGTCAATAGTGTCCCACAGATATAAGCACGCAGAATTCACACATCCCTATACCGAATCAGGATTGATAATGATAGTTCCGGTCAGGTCCAAAACATGTAGCAAAGCATGGTTATTTATGAGACCTTTCACAAAAGCAATGTGGGCACTGATAGGAGCGATCAATGTCTACAATGGCTTTGTCGTATGGCTGATAGAGCGAAACTATTGTCCTGAACTCAAAGGTTCTGTGCTAAATCAAATAGGAAGCTTGATTTGGCTATCCTTCACCACTCTCTTCTCCTTACACGATAAGCTGCATAGTAATTTGTCTAGGATGACAACGGTGGTCTGGCTATTTATGGCGCTAATCATCACGCAGACATACACGGCTAACTTTACCAGCATGCTTACTGTCCAGCAGCTTGAACCTACTGTAACAGATGTGGATGCGCTGCTGCGAACCAATGCCATGGTTGGTCATGGTAAGGGATCCTTTGTTTCAGCCTATTTAAGGGAAGTCTTGGGCTTCCACCCCAACAATATTTTGCAGTTTAACTCCTCAGAAGAATATGCCTTAGCCCTCAGAAGTAAAGTAATTGCTGCTGCCTTTCTTGAAGCCCCTTTGGCAAAAATATTCGTTGGAAAATACTGCAAAGCCTTTATGGCAGTTGGACCGACTTACAAAGTTGGAGGATTTGGATTTGTATTTCCAAAAGGGTCTCTGTTGCTTCCTAGTGTAACAGAAGCAATGCTAAAGGTAACCGAAAGTGGTGAACTTCGGATTTTAGAGAACAGCATGCTGGCCTCTCTGAAGTGCGTGGATGCAGAAACAGATGGTGCTATTGATAATCCTAGTCTAAGCCCCAAAAGCTTTTGGGTAGTATTCATACTCACAGGAGGAACATCGACAATTGCTCTCGTGGTTTATAGTTTTCGCATTTGTAAATCAATGCTTGAACACAAAACCAACTGGAAATTCATATTGGATGTAATGAAACAATGGGGAAGTCCAAACAGGAGATTCTCAAGAAGAGACAGCGATATGAATGAAGAAAGCCATGCATATCCTTCAAATGCATCTGCTGTGCAGGCTCAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

872

Amino Acids

97.56

Weight (kDa)

7.72

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 46 - 377 2.3e-22 Periplasmic binding protein
ANF_receptor PF01094 52 - 378 1.1e-51 Receptor family ligand binding region
SBP_bac_3 PF00497 462 - 768 1.3e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 556 - 805 2.9e-22 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 696
AccI GTMKAC 1 cut(s) 1701
AccIII TCCGGA 1 cut(s) 717
AciI CCGC 2 cut(s) 366, 864
AclI AACGTT 1 cut(s) 1334
AclWI GGATC 8 cut(s) 767, 770, 839, 1336, 1358, 1492, 1998, 2011
AcoI YGGCCR 1 cut(s) 1244
AcsI RAATTY 4 cut(s) 269, 1511, 1568, 2489
AcuI CTGAAG 3 cut(s) 516, 945, 2345
AfaI GTAC 2 cut(s) 684, 1346
AfiI CCNNNNNNNGG 7 cut(s) 104, 385, 863, 864, 928, 1469, 2025
AflIII ACRYGT 2 cut(s) 1105, 1628
AgeI ACCGGT 1 cut(s) 444
AjnI CCWGG 2 cut(s) 943, 1246
AjuI GAANNNNNNNTTGG 8 cut(s) 1352, 1384, 1771, 1803, 2474, 2480, 2506, 2512
Alw26I GTCTC 8 cut(s) 9, 320, 350, 565, 654, 1648, 2238, 2541
AlwI GGATC 8 cut(s) 767, 770, 839, 1336, 1358, 1492, 1998, 2011
Aor13HI TCCGGA 1 cut(s) 717
AoxI GGCC 4 cut(s) 416, 1244, 1253, 2316
ApoI RAATTY 4 cut(s) 269, 1511, 1568, 2489
ArsI GACNNNNNNTTYG 2 cut(s) 1726, 1758
AseI ATTAAT 1 cut(s) 632
AsiGI ACCGGT 1 cut(s) 444
AspLEI GCGC 3 cut(s) 697, 1879, 1966
AspS9I GGNCC 5 cut(s) 308, 417, 1253, 1620, 2186
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 5 cut(s) 757, 1205, 1541, 1792, 2297
AsuII TTCGAA 1 cut(s) 1467
AsuNHI GCTAGC 1 cut(s) 295
AvaII GGWCC 3 cut(s) 308, 1620, 2186
BaeGI GKGCMC 1 cut(s) 1681
BalI TGGCCA 1 cut(s) 1246
BamHI GGATCC 1 cut(s) 2003
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 2432
BbvCI CCTCAGC 1 cut(s) 384
BccI CCATC 4 cut(s) 736, 821, 1160, 2342
BceAI ACGGC 1 cut(s) 1917
BciT130I CCWGG 2 cut(s) 945, 1248
BciVI GTATCC 1 cut(s) 1148
BclI TGATCA 3 cut(s) 33, 181, 886
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 8 cut(s) 9, 320, 350, 565, 654, 1648, 2238, 2541
BfaI CTAG 7 cut(s) 110, 296, 1190, 1845, 2247, 2367, 2614
BfmI CTRYAG 2 cut(s) 474, 1521
BfoI RGCGCY 1 cut(s) 1880
BfuI GTATCC 1 cut(s) 1148
BglI GCCNNNNNGGC 3 cut(s) 1252, 2138, 2177
BglII AGATCT 2 cut(s) 1211, 1405
Bme1390I CCNGG 3 cut(s) 99, 945, 1248
Bme18I GGWCC 3 cut(s) 308, 1620, 2186
BmgT120I GGNCC 5 cut(s) 308, 417, 1253, 1620, 2186
BmiI GGNNCC 3 cut(s) 243, 1254, 2005
BmrFI CCNGG 3 cut(s) 99, 945, 1248
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 7 cut(s) 505, 553, 961, 1507, 1951, 2326, 2600
BmtI GCTAGC 1 cut(s) 299
BmuI ACTGGG 1 cut(s) 207
Bpu10I CCTNAGC 3 cut(s) 342, 384, 2089
Bpu14I TTCGAA 1 cut(s) 1467
BpuEI CTTGAG 5 cut(s) 589, 665, 827, 2524, 2592
BpuMI CCSGG 1 cut(s) 99
BsaAI YACGTR 2 cut(s) 223, 1348
BsaI GGTCTC 3 cut(s) 654, 1648, 2238
BsaJI CCNNGG 3 cut(s) 1247, 1256, 1983
BsaWI WCCGGW 3 cut(s) 444, 717, 1612
Bsc4I CCNNNNNNNGG 7 cut(s) 104, 385, 863, 864, 928, 1469, 2025
Bse118I RCCGGY 1 cut(s) 444
Bse1I ACTGG 5 cut(s) 202, 664, 705, 1163, 2489
Bse3DI GCAATG 2 cut(s) 1677, 2268
BseAI TCCGGA 1 cut(s) 717
BseBI CCWGG 2 cut(s) 945, 1248
BseDI CCNNGG 3 cut(s) 1247, 1256, 1983
BseGI GGATG 6 cut(s) 184, 1576, 1854, 1966, 2341, 2506
BseLI CCNNNNNNNGG 7 cut(s) 104, 385, 863, 864, 928, 1469, 2025
BseMI GCAATG 2 cut(s) 1677, 2268
BseMII CTCAG 7 cut(s) 20, 248, 375, 399, 1298, 2088, 2110
BseNI ACTGG 5 cut(s) 202, 664, 705, 1163, 2489
BseRI GAGGAG 2 cut(s) 944, 2062
BseSI GKGCMC 1 cut(s) 1681
BsgI GTGCAG 2 cut(s) 203, 603
BshFI GGCC 4 cut(s) 418, 1246, 1255, 2318
BshTI ACCGGT 1 cut(s) 444
BsiSI CCGG 4 cut(s) 99, 445, 718, 1613
BslFI GGGAC 3 cut(s) 120, 1386, 1531
BslI CCNNNNNNNGG 7 cut(s) 104, 385, 863, 864, 928, 1469, 2025
BsmAI GTCTC 8 cut(s) 9, 320, 350, 565, 654, 1648, 2238, 2541
BsmFI GGGAC 3 cut(s) 120, 1386, 1531
BsnI GGCC 4 cut(s) 418, 1246, 1255, 2318
Bso31I GGTCTC 3 cut(s) 654, 1648, 2238
Bsp119I TTCGAA 1 cut(s) 1467
Bsp1286I GDGCHC 2 cut(s) 246, 1681
Bsp13I TCCGGA 1 cut(s) 717
Bsp1407I TGTACA 1 cut(s) 682
Bsp19I CCATGG 1 cut(s) 1983
BspACI CCGC 2 cut(s) 366, 864
BspANI GGCC 4 cut(s) 418, 1246, 1255, 2318
BspCNI CTCAG 7 cut(s) 19, 247, 376, 400, 1299, 2087, 2109
BspEI TCCGGA 1 cut(s) 717
BspLI GGNNCC 3 cut(s) 243, 1254, 2005
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 8 cut(s) 767, 770, 839, 1336, 1358, 1492, 1998, 2011
BspQI GCTCTTC 2 cut(s) 21, 633
BspT104I TTCGAA 1 cut(s) 1467
BspTNI GGTCTC 3 cut(s) 654, 1648, 2238
BsrDI GCAATG 2 cut(s) 1677, 2268
BsrFI RCCGGY 1 cut(s) 444
BsrGI TGTACA 1 cut(s) 682
BsrI ACTGG 5 cut(s) 202, 664, 705, 1163, 2489
BssAI RCCGGY 1 cut(s) 444
BssECI CCNNGG 3 cut(s) 1247, 1256, 1983
BssSI CACGAG 1 cut(s) 2432
BssT1I CCWWGG 2 cut(s) 1256, 1983
Bst2BI CACGAG 1 cut(s) 2432
Bst2UI CCWGG 2 cut(s) 945, 1248
Bst4CI ACNGT 7 cut(s) 40, 131, 508, 1344, 1858, 1927, 1948
Bst6I CTCTTC 9 cut(s) 21, 144, 318, 633, 652, 921, 1415, 1814, 2539
BstAPI GCANNNNNTGC 3 cut(s) 260, 292, 470
BstAUI TGTACA 1 cut(s) 682
BstBAI YACGTR 2 cut(s) 223, 1348
BstBI TTCGAA 1 cut(s) 1467
BstC8I GCNNGC 8 cut(s) 252, 297, 1319, 1563, 1919, 2312, 2316, 2604
BstDSI CCRYGG 1 cut(s) 1983
BstEII GGTNACC 1 cut(s) 2272
BstENI CCTNNNNNAGG 2 cut(s) 383, 2023
BstF5I GGATG 6 cut(s) 184, 1576, 1854, 1966, 2341, 2506
BstH2I RGCGCY 1 cut(s) 1880
BstHHI GCGC 3 cut(s) 697, 1879, 1966
BstMAI GTCTC 8 cut(s) 9, 320, 350, 565, 654, 1648, 2238, 2541
BstNI CCWGG 2 cut(s) 945, 1248
BstNSI RCATGY 4 cut(s) 1109, 1632, 1921, 2314
BstPI GGTNACC 1 cut(s) 2272
BstSCI CCNGG 3 cut(s) 97, 943, 1246
BstSFI CTRYAG 2 cut(s) 474, 1521
BstSLI GKGCMC 1 cut(s) 1681
BstSNI TACGTA 1 cut(s) 1348
BstX2I RGATCY 5 cut(s) 1211, 1363, 1405, 1497, 2003
BstXI CCANNNNNNTGG 2 cut(s) 1275, 1984
BstYI RGATCY 5 cut(s) 1211, 1363, 1405, 1497, 2003
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 4 cut(s) 418, 1246, 1255, 2318
BtgI CCRYGG 1 cut(s) 1983
BtsCI GGATG 6 cut(s) 184, 1576, 1854, 1966, 2341, 2506
BtsI GCAGTG 3 cut(s) 358, 711, 990
Cac8I GCNNGC 8 cut(s) 252, 297, 1319, 1563, 1919, 2312, 2316, 2604
CfoI GCGC 3 cut(s) 697, 1879, 1966
Cfr10I RCCGGY 1 cut(s) 444
Cfr13I GGNCC 5 cut(s) 308, 417, 1253, 1620, 2186
Csp6I GTAC 2 cut(s) 683, 1345
CspAI ACCGGT 1 cut(s) 444
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviQI GTAC 2 cut(s) 683, 1345
DraI TTTAAA 1 cut(s) 1339
EaeI YGGCCR 1 cut(s) 1244
Eam1104I CTCTTC 9 cut(s) 21, 144, 318, 633, 652, 921, 1415, 1814, 2539
EarI CTCTTC 9 cut(s) 21, 144, 318, 633, 652, 921, 1415, 1814, 2539
Eco105I TACGTA 1 cut(s) 1348
Eco130I CCWWGG 2 cut(s) 1256, 1983
Eco24I GRGCYC 1 cut(s) 246
Eco31I GGTCTC 3 cut(s) 654, 1648, 2238
Eco47I GGWCC 3 cut(s) 308, 1620, 2186
Eco57I CTGAAG 3 cut(s) 516, 945, 2345
Eco91I GGTNACC 1 cut(s) 2272
EcoNI CCTNNNNNAGG 2 cut(s) 383, 2023
EcoO109I RGGNCCY 2 cut(s) 308, 1253
EcoO65I GGTNACC 1 cut(s) 2272
EcoRI GAATTC 1 cut(s) 1568
EcoRII CCWGG 2 cut(s) 943, 1246
EcoT14I CCWWGG 2 cut(s) 1256, 1983
EcoT22I ATGCAT 2 cut(s) 2578, 2593
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 1256, 1983
FalI AAGNNNNNCTT 2 cut(s) 1991, 2023
FaqI GGGAC 3 cut(s) 120, 1386, 1531
FauI CCCGC 1 cut(s) 857
FbaI TGATCA 3 cut(s) 33, 181, 886
FblI GTMKAC 1 cut(s) 1701
FokI GGATG 6 cut(s) 191, 1563, 1861, 1973, 2348, 2513
FriOI GRGCYC 1 cut(s) 246
FspBI CTAG 7 cut(s) 110, 296, 1190, 1845, 2247, 2367, 2614
FspI TGCGCA 1 cut(s) 696
GlaI GCGC 3 cut(s) 696, 1878, 1965
HaeII RGCGCY 1 cut(s) 1880
HaeIII GGCC 4 cut(s) 418, 1246, 1255, 2318
HapII CCGG 4 cut(s) 99, 445, 718, 1613
HhaI GCGC 3 cut(s) 697, 1879, 1966
Hin6I GCGC 3 cut(s) 695, 1877, 1964
HinP1I GCGC 3 cut(s) 695, 1877, 1964
HincII GTYRAC 1 cut(s) 1263
HindII GTYRAC 1 cut(s) 1263
HindIII AAGCTT 3 cut(s) 432, 1780, 2383
HpaII CCGG 4 cut(s) 99, 445, 718, 1613
HphI GGTGA 5 cut(s) 757, 1205, 1541, 1792, 2297
Hpy166II GTNNAC 6 cut(s) 793, 1196, 1263, 1278, 1702, 2288
Hpy188III TCNNGA 7 cut(s) 31, 718, 844, 1593, 1745, 2126, 2541
Hpy8I GTNNAC 6 cut(s) 793, 1196, 1263, 1278, 1702, 2288
HpyAV CCTTC 6 cut(s) 47, 715, 1463, 1474, 1807, 2592
HpyCH4III ACNGT 7 cut(s) 40, 131, 508, 1344, 1858, 1927, 1948
HpyCH4IV ACGT 5 cut(s) 222, 1198, 1302, 1334, 1347
HpySE526I ACGT 5 cut(s) 222, 1198, 1302, 1334, 1347
HspAI GCGC 3 cut(s) 695, 1877, 1964
Kpn2I TCCGGA 1 cut(s) 717
Ksp22I TGATCA 3 cut(s) 33, 181, 886
LguI GCTCTTC 2 cut(s) 21, 633
LmnI GCTCC 4 cut(s) 241, 1102, 1429, 1688
LweI GCATC 7 cut(s) 505, 553, 961, 1507, 1951, 2326, 2600
MaeI CTAG 7 cut(s) 110, 296, 1190, 1845, 2247, 2367, 2614
MaeII ACGT 5 cut(s) 222, 1198, 1302, 1334, 1347
MaeIII GTNAC 8 cut(s) 440, 502, 997, 1082, 1139, 1948, 2251, 2272
MfeI CAATTG 3 cut(s) 369, 465, 2424
MflI RGATCY 5 cut(s) 1211, 1363, 1405, 1497, 2003
MhlI GDGCHC 2 cut(s) 246, 1681
MlsI TGGCCA 1 cut(s) 1246
MluNI TGGCCA 1 cut(s) 1246
MlyI GAGTC 5 cut(s) 352, 681, 834, 1180, 1258
MmeI TCCRAC 4 cut(s) 1132, 2134, 2164, 2182
Mox20I TGGCCA 1 cut(s) 1246
Mph1103I ATGCAT 2 cut(s) 2578, 2593
MroI TCCGGA 1 cut(s) 717
MscI TGGCCA 1 cut(s) 1246
MseI TTAA 7 cut(s) 632, 801, 1061, 1074, 1338, 2024, 2067
MslI CAYNNNNRTG 1 cut(s) 1124
Msp20I TGGCCA 1 cut(s) 1246
MspI CCGG 4 cut(s) 99, 445, 718, 1613
MspR9I CCNGG 3 cut(s) 99, 945, 1248
MssI GTTTAAAC 1 cut(s) 1339
MunI CAATTG 3 cut(s) 369, 465, 2424
MvaI CCWGG 2 cut(s) 945, 1248
NciI CCSGG 1 cut(s) 99
NcoI CCATGG 1 cut(s) 1983
NheI GCTAGC 1 cut(s) 295
NlaIV GGNNCC 3 cut(s) 243, 1254, 2005
NmuCI GTSAC 3 cut(s) 502, 997, 1082
NsbI TGCGCA 1 cut(s) 696
NsiI ATGCAT 2 cut(s) 2578, 2593
NspI RCATGY 4 cut(s) 1109, 1632, 1921, 2314
NspV TTCGAA 1 cut(s) 1467
PaeI GCATGC 2 cut(s) 1921, 2314
PciI ACATGT 2 cut(s) 1105, 1628
PciSI GCTCTTC 2 cut(s) 21, 633
PfeI GAWTC 8 cut(s) 191, 202, 527, 948, 1176, 1481, 1589, 2535
PinAI ACCGGT 1 cut(s) 444
PleI GAGTC 5 cut(s) 352, 680, 834, 1180, 1258
PmeI GTTTAAAC 1 cut(s) 1339
PpsI GAGTC 5 cut(s) 352, 680, 834, 1180, 1258
Ppu21I YACGTR 2 cut(s) 223, 1348
PpuMI RGGWCCY 1 cut(s) 308
PscI ACATGT 2 cut(s) 1105, 1628
PshBI ATTAAT 1 cut(s) 632
Psp1406I AACGTT 1 cut(s) 1334
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 2 cut(s) 943, 1246
PspEI GGTNACC 1 cut(s) 2272
PspGI CCWGG 2 cut(s) 943, 1246
PspN4I GGNNCC 3 cut(s) 243, 1254, 2005
PspPI GGNCC 5 cut(s) 308, 417, 1253, 1620, 2186
PspPPI RGGWCCY 1 cut(s) 308
PsuI RGATCY 5 cut(s) 1211, 1363, 1405, 1497, 2003
RsaI GTAC 2 cut(s) 684, 1346
RsaNI GTAC 2 cut(s) 683, 1345
RseI CAYNNNNRTG 1 cut(s) 1124
SapI GCTCTTC 2 cut(s) 21, 633
SaqAI TTAA 7 cut(s) 632, 801, 1061, 1074, 1338, 2024, 2067
Sau96I GGNCC 5 cut(s) 308, 417, 1253, 1620, 2186
SchI GAGTC 5 cut(s) 352, 681, 834, 1180, 1258
ScrFI CCNGG 3 cut(s) 99, 945, 1248
SduI GDGCHC 2 cut(s) 246, 1681
SfaNI GCATC 7 cut(s) 505, 553, 961, 1507, 1951, 2326, 2600
SfcI CTRYAG 2 cut(s) 474, 1521
SfiI GGCCNNNNNGGCC 1 cut(s) 1252
SfuI TTCGAA 1 cut(s) 1467
SinI GGWCC 3 cut(s) 308, 1620, 2186
SmiMI CAYNNNNRTG 1 cut(s) 1124
SmlI CTYRAG 5 cut(s) 568, 644, 842, 2539, 2607
SmoI CTYRAG 5 cut(s) 568, 644, 842, 2539, 2607
SnaBI TACGTA 1 cut(s) 1348
SphI GCATGC 2 cut(s) 1921, 2314
SsiI CCGC 2 cut(s) 366, 864
SspI AATATT 3 cut(s) 1226, 2056, 2148
SspMI CTAG 7 cut(s) 110, 296, 1190, 1845, 2247, 2367, 2614
StyD4I CCNGG 3 cut(s) 97, 943, 1246
StyI CCWWGG 2 cut(s) 1256, 1983
TaaI ACNGT 7 cut(s) 40, 131, 508, 1344, 1858, 1927, 1948
TaiI ACGT 5 cut(s) 225, 1201, 1305, 1337, 1350
TaqI TCGA 3 cut(s) 123, 1467, 2420
TaqII GACCGA 1 cut(s) 2203
TatI WGTACW 1 cut(s) 682
TfiI GAWTC 8 cut(s) 191, 202, 527, 948, 1176, 1481, 1589, 2535
Tru1I TTAA 7 cut(s) 632, 801, 1061, 1074, 1338, 2024, 2067
Tru9I TTAA 7 cut(s) 632, 801, 1061, 1074, 1338, 2024, 2067
TseFI GTSAC 3 cut(s) 502, 997, 1082
Tsp45I GTSAC 3 cut(s) 502, 997, 1082
VpaK11BI GGWCC 3 cut(s) 308, 1620, 2186
VspI ATTAAT 1 cut(s) 632
XagI CCTNNNNNAGG 2 cut(s) 383, 2023
XapI RAATTY 4 cut(s) 269, 1511, 1568, 2489
XceI RCATGY 4 cut(s) 1109, 1632, 1921, 2314
XcmI CCANNNNNNNNNTGG 1 cut(s) 1254
XmiI GTMKAC 1 cut(s) 1701
XspI CTAG 7 cut(s) 110, 296, 1190, 1845, 2247, 2367, 2614
Zsp2I ATGCAT 2 cut(s) 2578, 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.