Rw5G020150

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010541
Physical Location & Seq
Forward (+)
26416205 .. 26419774
3570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020150.1

Sequence Viewer

Length: 2529 bp
ATGGGCGTCGTCGTTAACGTTGGGGCCATTATAGATGACAGTTCTCGAATTGGCAAAGAACAGAGAGTGGCAATGGAAATGGCAATGGAGGATTTTTGTGCAAATAACAGTCAGATGAGATTGGTTCTGCAAATAAAACACTCTCGGAGGGACCCCATGCAGGCGGCTCTTGCAGCTAGAGATCTCATAAATATGCAAGAAGCTCAAGCCATTCTAGGACCACATACATGGGAAGAGACATCATTAGTAGCTGCGGTTGGCAGTGAGAACCACATTCCAATTGTTTCTTTTGCGGAGGATACTCCAAACTGGGCAACAGAGCTCTGGCCCTTCCTAGTTCAAGCTTCTCATAACGAGCTAAAACAAATGGAAGCAGTAGCTGCTATAGTTAAGTCTTTTGAATGGTATCAGGTCACTGTTATATACCAAGATAGAGATTATTCGTCCAACGAAATTTTGCCTGGCCTCTCTCGTGCCTTGAGACAAGTTGGCTCAGAAATAAGCCAGCTCGTAGCTCTTCCCCCTTTCGGTTCTTCTTCATTAGTCAGAGAGCTTGAGAAGCTTCGACAAGACCAATATAGAGTTGTCATCGTGCATTTGTCTTTGCCGTTGGCATTGCAACTATTTGAAAAAGCTAAAGAAATGAAGATGATGGAAAAAGACTATGTATGGATCACCACAGATCCTATTACTAGCCTAGTACATTCCTTCAATGCCTCCACCATTTCCGCAATGCAGGGGGTTATCGGAGTCAAGAGCTTCTTCCCTGAAAGTGAGAGTCCCCAATTTCAGGGTTTTCATCGTAGATTTCGTAAAAGGTTCAGCTCAGAATATCCTGAAGAAGAAAACAATGATCCTGGGGTCTTTGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCAGTGACACAAAGGAAACACAATGGGAGGCCCTTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGTTTAAGTGGAAAAATTCAGTTCACTGACCAAAAATTACCACTACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTACAGAGAACTCGGATATTGGTCAGATGGATTAGGCTTCACACAAACTATCGGGGGAAATGATACTACCAGTTCATCCATGAAAGATTTTGGATCAGTGTTTTGGCCAGGGGGGACTTTCTATACTCCAAAAGGATGGACTGTATCAACAAGTTCAAAGCCATTGAGAATCGGAGTGCCTACCGGAGCGGCATTCAAACAGTATGCAAATGTAGAAAAAGATCCCTTGGGTAATAAATTCTTATTTGGTGGAATTGCAATTGATCTCTTCAAAGAAACCCTGATCGAGCTGCCTTATCATTTGCCGTATGAATTCTCCCCATTTAATGGCACATATGATTCATTAGTGCAGCAAATTCATTCTAGTACATTTGATGCTGTAGTTGGTGATGTAGCAATAGTGTCGCAAAGATATCAGTATGCAGAATTCACACAGCCCTATTCTGAAGCAGGACTGATGATGATAGTTCCAGTCAGGTCCAAATTTTGCAACAAAGCATGGTTATTCATGAACCCTTTCACACGAGCCATGTGGGCGCTCATAGTGGCCATCAATGTCTACAATGGTTTTGTCGTTTGGTTGATAGAGAGAAACTACTGCCCCGAACTCAGAGGTTCTCTGCCAAATCAAATTGGAACCTTGATTTGGCTTTCCTTCACCACTCTATTCTCCTTACATGGAGAAAAGCTACATAGCAATTTGTCAAGGATGACAATGGTGGTCTGGTTATTTGTCGCACTAGTCATCACACAGACATACACCGCCAACCTCACCAGCATGCTCACGGTCCAGCAACTTGAACCAGCTGTAGCAGATGTTGATGCACTTCTACAAAGCAATGCCGTTGTTGGTTATTGTAAGGGATCATTTGTTTCTATGTATCTAAAGAAAGTCTTGAAATTTTCCCCTGATCACATTCAGGAGTTTAGATCCCCGGAAGAGTATGCGAAAGCCCTCAAAAGCAAAGCAATTGCTGCTGCATTTCTTGAAGTCCCTTTTGCCAAGATATTCCTCGGACAATACTGCAAAGATTTTGTGTCGGTTGGGCCAACATTCAAAGTCGGAGGATTTGGATTCGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAAATGAAGCAATGCTGAAGGTGGCCGAAAGCGGTAAGCTTCGAGACTTGGAGAACGACATGCTGGCATCTCAGAAATGCTTGGATGCAGAAACAGATGGTAGAACCCCAAGTCTAAGCCCCAGCAGCTTTTGGGTACTATTCACATTCTCAGGAGGGACATCAACAATTGCTCTTCTGGTTTACATTTTCCGCATTTATAAATCGATGCTTAAGCACAAAACAATCTGGAACTTAATGTTGGCTGTAATCAACCAATGGGGGAATCCAAGTATGCAGAGGTTCTCAAGAAGAGACAGCGACATTAGTCCAGAAACTCCGACACATTCTGCAAATGTCCCTAACTTGCAGGCTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

842

Amino Acids

94.39

Weight (kDa)

6.98

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 5 - 334 7.8e-24 Periplasmic binding protein
ANF_receptor PF01094 23 - 350 6.1e-54 Receptor family ligand binding region
SBP_bac_3 PF00497 435 - 741 5.9e-09 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 528 - 772 1.5e-24 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2367
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccBSI CCGCTC 1 cut(s) 1244
AccI GTMKAC 1 cut(s) 1614
AciI CCGC 9 cut(s) 164, 254, 293, 729, 869, 1244, 1818, 2199, 2359
AclI AACGTT 1 cut(s) 18
AclWI GGATC 7 cut(s) 677, 680, 848, 1156, 1271, 1927, 1980
AcoI YGGCCR 3 cut(s) 1160, 1602, 2190
AcsI RAATTY 8 cut(s) 453, 987, 1292, 1367, 1410, 1481, 1538, 1955
AcuI CTGAAG 3 cut(s) 858, 1521, 2204
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 3 cut(s) 702, 1423, 2304
AfiI CCNNNNNNNGG 5 cut(s) 160, 527, 790, 1382, 1701
AflII CTTAAG 1 cut(s) 2378
AhlI ACTAGT 1 cut(s) 1795
AjnI CCWGG 3 cut(s) 460, 856, 1162
AjuI GAANNNNNNNTTGG 4 cut(s) 1684, 1716, 2390, 2422
Alw21I GWGCWC 1 cut(s) 324
Alw26I GTCTC 4 cut(s) 230, 475, 2205, 2454
AlwI GGATC 7 cut(s) 677, 680, 848, 1156, 1271, 1927, 1980
AlwNI CAGNNNCTG 1 cut(s) 380
AoxI GGCC 9 cut(s) 24, 326, 463, 876, 935, 1160, 1602, 2102, 2190
ApeKI GCWGC 9 cut(s) 173, 251, 380, 887, 1345, 1405, 2030, 2033, 2292
ApoI RAATTY 8 cut(s) 453, 987, 1292, 1367, 1410, 1481, 1538, 1955
Asp700I GAANNNNTTC 2 cut(s) 951, 1571
AspLEI GCGC 1 cut(s) 1594
AspS9I GGNCC 8 cut(s) 24, 151, 218, 327, 936, 1533, 1843, 2102
AsuC2I CCSGG 1 cut(s) 1991
AsuHPI GGTGA 5 cut(s) 667, 1454, 1705, 1819, 2142
AsuII TTCGAA 1 cut(s) 958
AvaII GGWCC 4 cut(s) 151, 218, 1533, 1843
BalI TGGCCA 2 cut(s) 1162, 1604
BanII GRGCYC 1 cut(s) 324
BauI CACGAG 2 cut(s) 471, 1578
Bbv12I GWGCWC 1 cut(s) 324
BbvI GCAGC 9 cut(s) 185, 238, 367, 874, 1332, 1417, 2017, 2020, 2304
BccI CCATC 5 cut(s) 646, 1076, 1185, 1613, 2258
BceAI ACGGC 3 cut(s) 592, 1345, 1883
BcgI CGANNNNNNTGC 2 cut(s) 1440, 1474
BciT130I CCWGG 3 cut(s) 462, 858, 1164
BciVI GTATCC 1 cut(s) 292
BclI TGATCA 1 cut(s) 1966
BcnI CCSGG 1 cut(s) 1991
BcoDI GTCTC 4 cut(s) 230, 475, 2205, 2454
BcuI ACTAGT 1 cut(s) 1795
BfaI CTAG 9 cut(s) 177, 215, 335, 693, 698, 905, 1419, 1796, 2527
BfmI CTRYAG 3 cut(s) 384, 1434, 1863
BfoI RGCGCY 1 cut(s) 1595
BfrI CTTAAG 1 cut(s) 2378
BfuI GTATCC 1 cut(s) 292
BglI GCCNNNNNGGC 1 cut(s) 1589
BglII AGATCT 1 cut(s) 181
Bme1390I CCNGG 4 cut(s) 462, 858, 1164, 1991
Bme18I GGWCC 4 cut(s) 151, 218, 1533, 1843
BmgT120I GGNCC 8 cut(s) 24, 151, 218, 327, 936, 1533, 1843, 2102
BmiI GGNNCC 4 cut(s) 25, 152, 153, 1693
BmrFI CCNGG 4 cut(s) 462, 858, 1164, 1991
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 6 cut(s) 874, 1420, 1867, 2242, 2243, 2364
BmuI ACTGGG 1 cut(s) 319
BoxI GACNNNNGTC 1 cut(s) 2472
Bpu10I CCTNAGC 1 cut(s) 2520
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 4 cut(s) 189, 499, 575, 2437
BpuMI CCSGG 1 cut(s) 1991
Bsa29I ATCGAT 1 cut(s) 2372
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 6 cut(s) 857, 1163, 1281, 1989, 2068, 2141
BsaWI WCCGGW 1 cut(s) 1238
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 527, 790, 1382, 1701
Bse1I ACTGG 3 cut(s) 314, 1125, 1526
Bse3DI GCAATG 6 cut(s) 78, 90, 614, 738, 1900, 2184
BseBI CCWGG 3 cut(s) 462, 858, 1164
BseCI ATCGAT 1 cut(s) 2372
BseDI CCNNGG 6 cut(s) 857, 1163, 1281, 1989, 2068, 2141
BseGI GGATG 4 cut(s) 1130, 1196, 1770, 2257
BseLI CCNNNNNNNGG 5 cut(s) 160, 527, 790, 1382, 1701
BseMI GCAATG 6 cut(s) 78, 90, 614, 738, 1900, 2184
BseMII CTCAG 5 cut(s) 507, 840, 1678, 2252, 2331
BseNI ACTGG 3 cut(s) 314, 1125, 1526
BseXI GCAGC 9 cut(s) 185, 238, 367, 874, 1332, 1417, 2017, 2020, 2304
BseYI CCCAGC 1 cut(s) 2288
BsgI GTGCAG 1 cut(s) 1424
BshFI GGCC 9 cut(s) 26, 328, 465, 878, 937, 1162, 1604, 2104, 2192
BshVI ATCGAT 1 cut(s) 2372
BsiHKAI GWGCWC 1 cut(s) 324
BsiSI CCGG 2 cut(s) 1239, 1991
BslFI GGGAC 6 cut(s) 164, 765, 1183, 2033, 2338, 2489
BslI CCNNNNNNNGG 5 cut(s) 160, 527, 790, 1382, 1701
BsmAI GTCTC 4 cut(s) 230, 475, 2205, 2454
BsmFI GGGAC 6 cut(s) 164, 765, 1183, 2033, 2338, 2489
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 9 cut(s) 26, 328, 465, 878, 937, 1162, 1604, 2104, 2192
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 1 cut(s) 324
BspACI CCGC 9 cut(s) 164, 254, 293, 729, 869, 1244, 1818, 2199, 2359
BspANI GGCC 9 cut(s) 26, 328, 465, 878, 937, 1162, 1604, 2104, 2192
BspCNI CTCAG 5 cut(s) 506, 839, 1677, 2251, 2330
BspDI ATCGAT 1 cut(s) 2372
BspHI TCATGA 1 cut(s) 1563
BspLI GGNNCC 4 cut(s) 25, 152, 153, 1693
BspPI GGATC 7 cut(s) 677, 680, 848, 1156, 1271, 1927, 1980
BspQI GCTCTTC 2 cut(s) 522, 2346
BspT104I TTCGAA 1 cut(s) 958
BspTI CTTAAG 1 cut(s) 2378
BsrBI CCGCTC 1 cut(s) 1244
BsrDI GCAATG 6 cut(s) 78, 90, 614, 738, 1900, 2184
BsrI ACTGG 3 cut(s) 314, 1125, 1526
BssECI CCNNGG 6 cut(s) 857, 1163, 1281, 1989, 2068, 2141
BssNI GRCGYC 1 cut(s) 6
BssSI CACGAG 2 cut(s) 471, 1578
BssT1I CCWWGG 2 cut(s) 1281, 2141
Bst2BI CACGAG 2 cut(s) 471, 1578
Bst2UI CCWGG 3 cut(s) 462, 858, 1164
Bst4CI ACNGT 6 cut(s) 41, 110, 418, 1198, 1257, 1843
Bst6I CTCTTC 6 cut(s) 228, 522, 1328, 1989, 2346, 2452
BstACI GRCGYC 1 cut(s) 6
BstAFI CTTAAG 1 cut(s) 2378
BstAPI GCANNNNNTGC 2 cut(s) 380, 2030
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 5 cut(s) 162, 506, 1835, 2232, 2517
BstDEI CTNAG 7 cut(s) 493, 826, 1664, 2238, 2282, 2317, 2520
BstEII GGTNACC 1 cut(s) 2148
BstF5I GGATG 4 cut(s) 1130, 1196, 1770, 2257
BstH2I RGCGCY 1 cut(s) 1595
BstHHI GCGC 1 cut(s) 1594
BstMAI GTCTC 4 cut(s) 230, 475, 2205, 2454
BstNI CCWGG 3 cut(s) 462, 858, 1164
BstNSI RCATGY 2 cut(s) 1837, 2230
BstPAI GACNNNNGTC 1 cut(s) 2472
BstPI GGTNACC 1 cut(s) 2148
BstSCI CCNGG 4 cut(s) 460, 856, 1162, 1989
BstSFI CTRYAG 3 cut(s) 384, 1434, 1863
BstV1I GCAGC 9 cut(s) 185, 238, 367, 874, 1332, 1417, 2017, 2020, 2304
BstX2I RGATCY 4 cut(s) 181, 682, 1276, 1985
BstXI CCANNNNNNTGG 2 cut(s) 228, 1191
BstYI RGATCY 4 cut(s) 181, 682, 1276, 1985
Bsu15I ATCGAT 1 cut(s) 2372
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 9 cut(s) 26, 328, 465, 878, 937, 1162, 1604, 2104, 2192
BsuTUI ATCGAT 1 cut(s) 2372
BtsCI GGATG 4 cut(s) 1130, 1196, 1770, 2257
BtsI GCAGTG 3 cut(s) 268, 903, 915
BtsIMutI CAGTG 6 cut(s) 268, 414, 903, 915, 996, 1158
Cac8I GCNNGC 5 cut(s) 162, 506, 1835, 2232, 2517
CaiI CAGNNNCTG 1 cut(s) 380
CciI TCATGA 1 cut(s) 1563
CfoI GCGC 1 cut(s) 1594
Cfr13I GGNCC 8 cut(s) 24, 151, 218, 327, 936, 1533, 1843, 2102
ClaI ATCGAT 1 cut(s) 2372
Csp6I GTAC 3 cut(s) 701, 1422, 2303
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviQI GTAC 3 cut(s) 701, 1422, 2303
DdeI CTNAG 7 cut(s) 493, 826, 1664, 2238, 2282, 2317, 2520
EaeI YGGCCR 3 cut(s) 1160, 1602, 2190
Eam1104I CTCTTC 6 cut(s) 228, 522, 1328, 1989, 2346, 2452
EarI CTCTTC 6 cut(s) 228, 522, 1328, 1989, 2346, 2452
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 2 cut(s) 1281, 2141
Eco147I AGGCCT 1 cut(s) 878
Eco24I GRGCYC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 1469
Eco47I GGWCC 4 cut(s) 151, 218, 1533, 1843
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 3 cut(s) 858, 1521, 2204
Eco91I GGTNACC 1 cut(s) 2148
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 151, 936
EcoO65I GGTNACC 1 cut(s) 2148
EcoRI GAATTC 2 cut(s) 1367, 1481
EcoRII CCWGG 3 cut(s) 460, 856, 1162
EcoRV GATATC 1 cut(s) 1469
EcoT14I CCWWGG 2 cut(s) 1281, 2141
EcoT38I GRGCYC 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 1281, 2141
FalI AAGNNNNNCTT 4 cut(s) 746, 778, 1934, 1966
FaqI GGGAC 6 cut(s) 164, 765, 1183, 2033, 2338, 2489
FauNDI CATATG 1 cut(s) 1390
FbaI TGATCA 1 cut(s) 1966
FblI GTMKAC 1 cut(s) 1614
FokI GGATG 4 cut(s) 1117, 1203, 1777, 2264
FriOI GRGCYC 1 cut(s) 324
FspBI CTAG 9 cut(s) 177, 215, 335, 693, 698, 905, 1419, 1796, 2527
GlaI GCGC 1 cut(s) 1593
GsaI CCCAGC 1 cut(s) 2292
HaeII RGCGCY 1 cut(s) 1595
HaeIII GGCC 9 cut(s) 26, 328, 465, 878, 937, 1162, 1604, 2104, 2192
HapII CCGG 2 cut(s) 1239, 1991
HhaI GCGC 1 cut(s) 1594
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 1 cut(s) 1592
HinP1I GCGC 1 cut(s) 1592
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 3 cut(s) 342, 560, 2204
HinfI GANTC 6 cut(s) 750, 778, 1224, 1394, 2130, 2431
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 2 cut(s) 1239, 1991
HphI GGTGA 5 cut(s) 667, 1454, 1705, 1819, 2142
Hpy166II GTNNAC 4 cut(s) 16, 996, 1615, 2350
Hpy8I GTNNAC 4 cut(s) 16, 996, 1615, 2350
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 4 cut(s) 340, 718, 1720, 2179
HpyCH4III ACNGT 6 cut(s) 41, 110, 418, 1198, 1257, 1843
HpyCH4IV ACGT 1 cut(s) 18
HpyF3I CTNAG 7 cut(s) 493, 826, 1664, 2238, 2282, 2317, 2520
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 1 cut(s) 1592
KflI GGGWCCC 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 1966
KspAI GTTAAC 1 cut(s) 16
LguI GCTCTTC 2 cut(s) 522, 2346
LmnI GCTCC 1 cut(s) 1241
Lsp1109I GCAGC 9 cut(s) 185, 238, 367, 874, 1332, 1417, 2017, 2020, 2304
LweI GCATC 6 cut(s) 874, 1420, 1867, 2242, 2243, 2364
MaeI CTAG 9 cut(s) 177, 215, 335, 693, 698, 905, 1419, 1796, 2527
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 4 cut(s) 412, 910, 1055, 2148
MbiI CCGCTC 1 cut(s) 1244
MfeI CAATTG 4 cut(s) 279, 1314, 2025, 2334
MflI RGATCY 4 cut(s) 181, 682, 1276, 1985
MhlI GDGCHC 1 cut(s) 324
MlsI TGGCCA 2 cut(s) 1162, 1604
MluNI TGGCCA 2 cut(s) 1162, 1604
MlyI GAGTC 2 cut(s) 759, 787
MmeI TCCRAC 4 cut(s) 471, 924, 2098, 2510
Mox20I TGGCCA 2 cut(s) 1162, 1604
MroXI GAANNNNTTC 2 cut(s) 951, 1571
MscI TGGCCA 2 cut(s) 1162, 1604
MseI TTAA 6 cut(s) 15, 390, 977, 1380, 2379, 2402
MslI CAYNNNNRTG 3 cut(s) 191, 226, 1832
Msp20I TGGCCA 2 cut(s) 1162, 1604
MspA1I CMGCKG 1 cut(s) 1862
MspCI CTTAAG 1 cut(s) 2378
MspI CCGG 2 cut(s) 1239, 1991
MspR9I CCNGG 4 cut(s) 462, 858, 1164, 1991
MunI CAATTG 4 cut(s) 279, 1314, 2025, 2334
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 3 cut(s) 462, 858, 1164
NciI CCSGG 1 cut(s) 1991
NdeI CATATG 1 cut(s) 1390
NlaIV GGNNCC 4 cut(s) 25, 152, 153, 1693
NmuCI GTSAC 3 cut(s) 412, 910, 2148
NspI RCATGY 2 cut(s) 1837, 2230
NspV TTCGAA 1 cut(s) 958
PaeI GCATGC 1 cut(s) 1837
PagI TCATGA 1 cut(s) 1563
PceI AGGCCT 1 cut(s) 878
PciSI GCTCTTC 2 cut(s) 522, 2346
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 808
PctI GAATGC 1 cut(s) 1247
PdmI GAANNNNTTC 2 cut(s) 951, 1571
PfeI GAWTC 4 cut(s) 1224, 1394, 2130, 2431
PflMI CCANNNNNTGG 1 cut(s) 1382
PleI GAGTC 2 cut(s) 758, 786
PpsI GAGTC 2 cut(s) 758, 786
PpuMI RGGWCCY 1 cut(s) 151
PshAI GACNNNNGTC 1 cut(s) 2472
PsiI TTATAA 1 cut(s) 2367
Psp124BI GAGCTC 1 cut(s) 324
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 3 cut(s) 460, 856, 1162
PspEI GGTNACC 1 cut(s) 2148
PspFI CCCAGC 1 cut(s) 2288
PspGI CCWGG 3 cut(s) 460, 856, 1162
PspN4I GGNNCC 4 cut(s) 25, 152, 153, 1693
PspPI GGNCC 8 cut(s) 24, 151, 218, 327, 936, 1533, 1843, 2102
PspPPI RGGWCCY 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 4 cut(s) 181, 682, 1276, 1985
PvuII CAGCTG 1 cut(s) 1862
RsaI GTAC 3 cut(s) 702, 1423, 2304
RsaNI GTAC 3 cut(s) 701, 1422, 2303
RseI CAYNNNNRTG 3 cut(s) 191, 226, 1832
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 2 cut(s) 522, 2346
SaqAI TTAA 6 cut(s) 15, 390, 977, 1380, 2379, 2402
Sau96I GGNCC 8 cut(s) 24, 151, 218, 327, 936, 1533, 1843, 2102
SchI GAGTC 2 cut(s) 759, 787
ScrFI CCNGG 4 cut(s) 462, 858, 1164, 1991
SduI GDGCHC 1 cut(s) 324
SfaNI GCATC 6 cut(s) 874, 1420, 1867, 2242, 2243, 2364
SfcI CTRYAG 3 cut(s) 384, 1434, 1863
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 4 cut(s) 151, 218, 1533, 1843
SmiMI CAYNNNNRTG 3 cut(s) 191, 226, 1832
SmlI CTYRAG 5 cut(s) 204, 478, 554, 2378, 2452
SmoI CTYRAG 5 cut(s) 204, 478, 554, 2378, 2452
SpeI ACTAGT 1 cut(s) 1795
SphI GCATGC 1 cut(s) 1837
SseBI AGGCCT 1 cut(s) 878
SsiI CCGC 9 cut(s) 164, 254, 293, 729, 869, 1244, 1818, 2199, 2359
SspI AATATT 1 cut(s) 952
SspMI CTAG 9 cut(s) 177, 215, 335, 693, 698, 905, 1419, 1796, 2527
SstI GAGCTC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 4 cut(s) 460, 856, 1162, 1989
StyI CCWWGG 2 cut(s) 1281, 2141
TaaI ACNGT 6 cut(s) 41, 110, 418, 1198, 1257, 1843
TaiI ACGT 1 cut(s) 21
TaqI TCGA 6 cut(s) 46, 565, 958, 1341, 2209, 2372
TatI WGTACW 2 cut(s) 700, 1421
TauI GCSGC 3 cut(s) 167, 872, 1247
TfiI GAWTC 4 cut(s) 1224, 1394, 2130, 2431
Tru1I TTAA 6 cut(s) 15, 390, 977, 1380, 2379, 2402
Tru9I TTAA 6 cut(s) 15, 390, 977, 1380, 2379, 2402
TscAI CASTG 6 cut(s) 268, 421, 903, 915, 1003, 1158
TseFI GTSAC 3 cut(s) 412, 910, 2148
TseI GCWGC 9 cut(s) 173, 251, 380, 887, 1345, 1405, 2030, 2033, 2292
Tsp45I GTSAC 3 cut(s) 412, 910, 2148
TspRI CASTG 6 cut(s) 268, 421, 903, 915, 1003, 1158
Van91I CCANNNNNTGG 1 cut(s) 1382
Vha464I CTTAAG 1 cut(s) 2378
VpaK11BI GGWCC 4 cut(s) 151, 218, 1533, 1843
XapI RAATTY 8 cut(s) 453, 987, 1292, 1367, 1410, 1481, 1538, 1955
XceI RCATGY 2 cut(s) 1837, 2230
XmiI GTMKAC 1 cut(s) 1614
XmnI GAANNNNTTC 2 cut(s) 951, 1571
XspI CTAG 9 cut(s) 177, 215, 335, 693, 698, 905, 1419, 1796, 2527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.