Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0010541

Physical Location & Seq

Genomic Coordinates Forward (+)

25823857 .. 25828408

Gene Structure 4552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033331

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGGGCGTCGTCGTTAACGTTGGGGCCATTATAGATAACAGTTCTCGAATTGGCAAAGAACAGAGAGTGGCAATGGAAATGGCAATGGAGGATTTTTGTGCAAATAACAATCAGAGGTTGGTTCTACAAATAAAACACTCTCAGAGGGACCCCATGCAGGCGGCTCTTGCAGCTAGAGATCTCATAAATATGCAAGAAGTTCAAGCCATTCTAGGACCACATACATGGGAAGAGACATCATTAGTAGCTGGGGTTGGCAGTGAGAACCACATTCCAATTGTTTCTTTTGCGGAGGCTACTCCAAACTGGGCAACAGAGCTCTGGCCCTTCCTAGTTCAAGCTTCTCATAACGAGCTAAAACAAATGGAAGCAGTAGCTGCTATAGTTAAGTCTTTTGAATGGTATCAGGTCGCTGTTATATACCAAGATAAAGATTATTCGTCCAACGAAATTTTGCCTGGCCTCTCTCGTGCCTTGAGACAAGTTGGCTCAGAAATAAGCCAGCTCGTAGCTCTTCCCCCTTTCGGTTCTTCTTCATTAGTCAGAGAGCTTGAGAAGCTTCGACAAGACCAATATAGAGTTGTCATCGTGCATTTGTCTTTGCCGTTGGCATTGCAAGTATTTGAAAAAGCTATAGAAATGAAGATGATGGAAAAAGACTATGTATGGATAACCACAGATCCTATTACTAGCCTAGTACATTCCTTCAATGCCTCCACCATTTCCGCAATGCAGGGGGTTATCGGAGTCAAAAGCTACTTCCCTGAATATGAGAGTACCCAATTTCAGGGTTTTCATCGTAGATTTCGTAAAAGGTTCAGCTTAGAATATCCTGAAGAAGAAAACAATGAGCCTGGGGTCTTCGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCATTGAGACAAAGAAAACACAATGGGAGGCCCCTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGCTTAAGTGGAAAAATTCAGTTCACTGACCAAAAATTACCACTACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTATAGAGAACTCGGATATTGGTCAGATGGATTAGGCTTCACACAGACTAAAGGGGGAAATTATACTACCAGCTCATCCATGAAAGATTTTGGACCAGTGTTTTGGCCAGGGGGGTCTTTCTATACTCCAAAAGGATGGACTCTATCAACAAGTTCAAATCCATTGAGAATCGGCGTGCCTAGCGGAGCGACATTCAAACAGTATGCAAATGTAGAAAAAGATCCCTTGGGTAATAACTTCTTATTTGGTGGAATTGCAATTGATCTCTTCGAAGAAACCCTAATCGAGCTGCCTTATCATTTGCCATATGATTTCTTCCCATTTAATGGCACATATGATTCATTAGTGCAGCAAATTCATTCTAGTGTAAGAGAAAAGCTACATAGCAATTTGTCAAGGATGACAATGGTGGTCTGGTTATTTGTCGCACTAGTCATCACACAGACATACACCGCCAACCTCACCAGCATGCTTACGGTCCAGCAACTTGAACCAGCTGTAGCAGATGTTAATGCACTTCTACAAAGCAATGCCGTTGTTGGTTATTGTAAAGGATCATTTGTTTCTATGTATCTAAAGAAAGTCTTGAAATTTTCCCCTGATCACATTCAGGAGTTTAGCTCCCCGGAAGAGTATGCAAAAGCCCTCAAAAGCAAAGCAATTGCTGCTGCATTTCTTGAAGTCCCTTTTGCCAAGATATTCCTCAGACAATACTGCAAAGATTTTGTGTCGGTTGGGCCAACATTCAAAGTCGGAGGATTTGGATTCGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAAATGAAGCAATGCTGAAGGTGGGCGAAAGTGGTAAGCTTCGAGATTTGGAGAACAACATGCTGGCATCTCAGAAATGCTTGGATGCAGAAACAGATGGTAGAACCCCAAGTCTAAGCCCCAGCAGCTTTTGGGTACTATTCACATTCTCAGGAGGGACATCAACAATTGCTCTTCTGGTTTACATTTTCCGCATTTATAAATCGATGCTTAAGCACAAAACAGTCTGGAACTTAATGCTGGCTGTAGTCAACCAATGGGGGAATCCAAGCATGCAGAGGTTCTCAAGAAGAGATAGCGACATTAATCCAGAAACTCCGACACATTCTGCAAATGTCCCTAACTTGCAGACTCAGATTGGACCCCTAACACTGACTCTCTACCGTCTCGCCGTCGAAGTATATGGAGAGGAGACAGCTGATGAATTGCAAACTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

765

Amino Acids

85.62

Weight (kDa)

6.46

Isoelectric Point (pI)

38.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	5 - 334	2.9e-25	Periplasmic binding protein
ANF_receptor	PF01094	23 - 349	1.1e-54	Receptor family ligand binding region
Lig_chan	PF00060	472 - 668	2.7e-16	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010541)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g18830 FvH4_3g18830 FvH4_3g18830
malus_domestica	MD03G1237700.v1.1 MD03G1237800.v1.1 MD11G1258300.v1.1
prunus_persica	Prupe.4G171700_v2.0.a1 Prupe.4G171800_v2.0.a1 Prupe.4G171900_v2.0.a1
pyrus_communis	pycom03g18340 pycom11g22780
rosa_chinensis	RchiOBHm_Chr5g0031641
rosa_laevigata	RLG00000033331
rosa_roxburghii	Rroxscaffold_1G00048630
rosa_rugosa	Rorug05G0127200
rosa_samantha	Rh5AG221100 Rh5BG220600 Rh5BG220700 Rh5CG244100 Rh5DG225600
rosa_wichuraiana	Rw5G020150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2055
AccB7I	CCANNNNNTGG	1 cut(s)	1379
AccII	CGCG	1 cut(s)	866
AciI	CCGC	7 cut(s)	161, 290, 726, 866, 1236, 1506, 2047
AclI	AACGTT	1 cut(s)	18
AclWI	GGATC	3 cut(s)	674, 1268, 1615
AcoI	YGGCCR	1 cut(s)	1157
AcsI	RAATTY	4 cut(s)	450, 984, 1407, 1643
AcuI	CTGAAG	2 cut(s)	855, 1892
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	3 cut(s)	699, 778, 1992
AfiI	CCNNNNNNNGG	4 cut(s)	157, 524, 787, 1379
AflII	CTTAAG	2 cut(s)	973, 2066
AhlI	ACTAGT	1 cut(s)	1483
AjnI	CCWGG	3 cut(s)	457, 853, 1159
Alw21I	GWGCWC	1 cut(s)	321
Alw26I	GTCTC	5 cut(s)	227, 472, 904, 2246, 2261
AlwI	GGATC	3 cut(s)	674, 1268, 1615
AlwNI	CAGNNNCTG	1 cut(s)	377
AoxI	GGCC	7 cut(s)	24, 323, 460, 873, 932, 1157, 1790
ApeKI	GCWGC	8 cut(s)	170, 377, 884, 1342, 1402, 1718, 1721, 1980
ApoI	RAATTY	4 cut(s)	450, 984, 1407, 1643
ArsI	GACNNNNNNTTYG	2 cut(s)	1137, 1169
AseI	ATTAAT	1 cut(s)	2160
Asp700I	GAANNNNTTC	1 cut(s)	948
AspS9I	GGNCC	9 cut(s)	24, 148, 215, 324, 933, 1145, 1531, 1790, 2216
AsuC2I	CCSGG	1 cut(s)	1679
AsuHPI	GGTGA	2 cut(s)	1507, 1830
AsuII	TTCGAA	2 cut(s)	955, 1323
AvaII	GGWCC	5 cut(s)	148, 215, 1145, 1531, 2216
BalI	TGGCCA	1 cut(s)	1159
BanII	GRGCYC	1 cut(s)	321
BauI	CACGAG	1 cut(s)	468
BbsI	GAAGAC	1 cut(s)	853
Bbv12I	GWGCWC	1 cut(s)	321
BbvI	GCAGC	8 cut(s)	182, 364, 871, 1329, 1414, 1705, 1708, 1992
BccI	CCATC	4 cut(s)	643, 1073, 1182, 1946
BceAI	ACGGC	3 cut(s)	589, 1571, 2231
BciT130I	CCWGG	3 cut(s)	459, 855, 1161
BclI	TGATCA	1 cut(s)	1654
BcnI	CCSGG	1 cut(s)	1679
BcoDI	GTCTC	5 cut(s)	227, 472, 904, 2246, 2261
BcuI	ACTAGT	1 cut(s)	1483
BfaI	CTAG	9 cut(s)	174, 212, 332, 690, 695, 902, 1233, 1416, 1484
BfmI	CTRYAG	4 cut(s)	381, 633, 1551, 2100
BfrI	CTTAAG	2 cut(s)	973, 2066
BglII	AGATCT	1 cut(s)	178
Bme1390I	CCNGG	4 cut(s)	459, 855, 1161, 1679
Bme18I	GGWCC	5 cut(s)	148, 215, 1145, 1531, 2216
BmgT120I	GGNCC	9 cut(s)	24, 148, 215, 324, 933, 1145, 1531, 1790, 2216
BmiI	GGNNCC	5 cut(s)	25, 149, 150, 935, 2218
BmrFI	CCNGG	4 cut(s)	459, 855, 1161, 1679
BmrI	ACTGGG	1 cut(s)	316
BmsI	GCATC	4 cut(s)	871, 1930, 1931, 2052
BmuI	ACTGGG	1 cut(s)	316
BpiI	GAAGAC	1 cut(s)	853
BplI	GAGNNNNNCTC	2 cut(s)	1658, 1690
Bpu14I	TTCGAA	2 cut(s)	955, 1323
BpuEI	CTTGAG	3 cut(s)	496, 572, 2125
BpuMI	CCSGG	1 cut(s)	1679
Bsa29I	ATCGAT	1 cut(s)	2060
BsaHI	GRCGYC	1 cut(s)	6
BsaJI	CCNNGG	5 cut(s)	854, 1160, 1278, 1677, 1829
Bsc4I	CCNNNNNNNGG	4 cut(s)	157, 524, 787, 1379
Bse1I	ACTGG	2 cut(s)	311, 1148
Bse3DI	GCAATG	6 cut(s)	78, 90, 611, 735, 1588, 1872
BseBI	CCWGG	3 cut(s)	459, 855, 1161
BseCI	ATCGAT	1 cut(s)	2060
BseDI	CCNNGG	5 cut(s)	854, 1160, 1278, 1677, 1829
BseGI	GGATG	4 cut(s)	1127, 1193, 1458, 1945
BseLI	CCNNNNNNNGG	4 cut(s)	157, 524, 787, 1379
BseMI	GCAATG	6 cut(s)	78, 90, 611, 735, 1588, 1872
BseMII	CTCAG	6 cut(s)	155, 504, 1771, 1940, 2019, 2222
BseNI	ACTGG	2 cut(s)	311, 1148
BseRI	GAGGAG	1 cut(s)	2279
BseXI	GCAGC	8 cut(s)	182, 364, 871, 1329, 1414, 1705, 1708, 1992
BseYI	CCCAGC	2 cut(s)	248, 1976
BsgI	GTGCAG	1 cut(s)	1421
Bsh1236I	CGCG	1 cut(s)	866
BshFI	GGCC	7 cut(s)	26, 325, 462, 875, 934, 1159, 1792
BshVI	ATCGAT	1 cut(s)	2060
BsiHKAI	GWGCWC	1 cut(s)	321
BsiSI	CCGG	1 cut(s)	1679
BslFI	GGGAC	4 cut(s)	161, 1721, 2026, 2177
BslI	CCNNNNNNNGG	4 cut(s)	157, 524, 787, 1379
BsmAI	GTCTC	5 cut(s)	227, 472, 904, 2246, 2261
BsmBI	CGTCTC	1 cut(s)	2246
BsmFI	GGGAC	4 cut(s)	161, 1721, 2026, 2177
BsnI	GGCC	7 cut(s)	26, 325, 462, 875, 934, 1159, 1792
Bsp119I	TTCGAA	2 cut(s)	955, 1323
Bsp1286I	GDGCHC	1 cut(s)	321
Bsp143I	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
BspACI	CCGC	7 cut(s)	161, 290, 726, 866, 1236, 1506, 2047
BspANI	GGCC	7 cut(s)	26, 325, 462, 875, 934, 1159, 1792
BspCNI	CTCAG	6 cut(s)	154, 503, 1770, 1939, 2018, 2221
BspDI	ATCGAT	1 cut(s)	2060
BspFNI	CGCG	1 cut(s)	866
BspLI	GGNNCC	5 cut(s)	25, 149, 150, 935, 2218
BspPI	GGATC	3 cut(s)	674, 1268, 1615
BspQI	GCTCTTC	2 cut(s)	519, 2034
BspT104I	TTCGAA	2 cut(s)	955, 1323
BspTI	CTTAAG	2 cut(s)	973, 2066
BsrDI	GCAATG	6 cut(s)	78, 90, 611, 735, 1588, 1872
BsrI	ACTGG	2 cut(s)	311, 1148
BssECI	CCNNGG	5 cut(s)	854, 1160, 1278, 1677, 1829
BssMI	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
BssNI	GRCGYC	1 cut(s)	6
BssSI	CACGAG	1 cut(s)	468
BssT1I	CCWWGG	2 cut(s)	1278, 1829
Bst2BI	CACGAG	1 cut(s)	468
Bst2UI	CCWGG	3 cut(s)	459, 855, 1161
Bst4CI	ACNGT	5 cut(s)	41, 1254, 1531, 2080, 2240
Bst6I	CTCTTC	6 cut(s)	225, 519, 1325, 1677, 2034, 2140
BstACI	GRCGYC	1 cut(s)	6
BstAFI	CTTAAG	2 cut(s)	973, 2066
BstAPI	GCANNNNNTGC	2 cut(s)	377, 1718
BstBI	TTCGAA	2 cut(s)	955, 1323
BstC8I	GCNNGC	7 cut(s)	159, 503, 1229, 1523, 1920, 2097, 2129
BstDEI	CTNAG	8 cut(s)	141, 490, 823, 1757, 1926, 1970, 2005, 2208
BstEII	GGTNACC	1 cut(s)	1836
BstF5I	GGATG	4 cut(s)	1127, 1193, 1458, 1945
BstFNI	CGCG	1 cut(s)	866
BstKTI	GATC	7 cut(s)	181, 682, 1032, 1276, 1318, 1610, 1657
BstMAI	GTCTC	5 cut(s)	227, 472, 904, 2246, 2261
BstMBI	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
BstNI	CCWGG	3 cut(s)	459, 855, 1161
BstNSI	RCATGY	3 cut(s)	1525, 1918, 2131
BstPI	GGTNACC	1 cut(s)	1836
BstSCI	CCNGG	4 cut(s)	457, 853, 1159, 1677
BstSFI	CTRYAG	4 cut(s)	381, 633, 1551, 2100
BstUI	CGCG	1 cut(s)	866
BstV1I	GCAGC	8 cut(s)	182, 364, 871, 1329, 1414, 1705, 1708, 1992
BstV2I	GAAGAC	1 cut(s)	853
BstX2I	RGATCY	3 cut(s)	178, 679, 1273
BstXI	CCANNNNNNTGG	3 cut(s)	225, 1155, 1188
BstYI	RGATCY	3 cut(s)	178, 679, 1273
Bsu15I	ATCGAT	1 cut(s)	2060
BsuRI	GGCC	7 cut(s)	26, 325, 462, 875, 934, 1159, 1792
BsuTUI	ATCGAT	1 cut(s)	2060
BtsCI	GGATG	4 cut(s)	1127, 1193, 1458, 1945
BtsI	GCAGTG	2 cut(s)	265, 900
BtsIMutI	CAGTG	5 cut(s)	265, 900, 993, 1155, 2225
Cac8I	GCNNGC	7 cut(s)	159, 503, 1229, 1523, 1920, 2097, 2129
CaiI	CAGNNNCTG	1 cut(s)	377
Cfr13I	GGNCC	9 cut(s)	24, 148, 215, 324, 933, 1145, 1531, 1790, 2216
ClaI	ATCGAT	1 cut(s)	2060
Csp6I	GTAC	3 cut(s)	698, 777, 1991
CspCI	CAANNNNNGTGG	2 cut(s)	876, 911
CviAII	CATG	7 cut(s)	154, 225, 968, 1132, 1522, 1915, 2128
CviQI	GTAC	3 cut(s)	698, 777, 1991
DdeI	CTNAG	8 cut(s)	141, 490, 823, 1757, 1926, 1970, 2005, 2208
DpnI	GATC	7 cut(s)	180, 681, 1031, 1275, 1317, 1609, 1656
DpnII	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
EaeI	YGGCCR	1 cut(s)	1157
Eam1104I	CTCTTC	6 cut(s)	225, 519, 1325, 1677, 2034, 2140
EarI	CTCTTC	6 cut(s)	225, 519, 1325, 1677, 2034, 2140
Ecl136II	GAGCTC	1 cut(s)	319
Eco130I	CCWWGG	2 cut(s)	1278, 1829
Eco147I	AGGCCT	1 cut(s)	875
Eco24I	GRGCYC	1 cut(s)	321
Eco47I	GGWCC	5 cut(s)	148, 215, 1145, 1531, 2216
Eco53kI	GAGCTC	1 cut(s)	319
Eco57I	CTGAAG	2 cut(s)	855, 1892
Eco91I	GGTNACC	1 cut(s)	1836
EcoICRI	GAGCTC	1 cut(s)	319
EcoO109I	RGGNCCY	2 cut(s)	148, 933
EcoO65I	GGTNACC	1 cut(s)	1836
EcoRII	CCWGG	3 cut(s)	457, 853, 1159
EcoT14I	CCWWGG	2 cut(s)	1278, 1829
EcoT38I	GRGCYC	1 cut(s)	321
ErhI	CCWWGG	2 cut(s)	1278, 1829
Esp3I	CGTCTC	1 cut(s)	2246
FaeI	CATG	7 cut(s)	157, 228, 971, 1135, 1525, 1918, 2131
FalI	AAGNNNNNCTT	2 cut(s)	1622, 1654
FaqI	GGGAC	4 cut(s)	161, 1721, 2026, 2177
FatI	CATG	7 cut(s)	153, 224, 967, 1131, 1521, 1914, 2127
FauNDI	CATATG	2 cut(s)	1360, 1387
FbaI	TGATCA	1 cut(s)	1654
FokI	GGATG	4 cut(s)	1114, 1200, 1465, 1952
FriOI	GRGCYC	1 cut(s)	321
FspBI	CTAG	9 cut(s)	174, 212, 332, 690, 695, 902, 1233, 1416, 1484
GsaI	CCCAGC	2 cut(s)	252, 1980
HaeIII	GGCC	7 cut(s)	26, 325, 462, 875, 934, 1159, 1792
HapII	CCGG	1 cut(s)	1679
Hin1I	GRCGYC	1 cut(s)	6
Hin1II	CATG	7 cut(s)	157, 228, 971, 1135, 1525, 1918, 2131
HincII	GTYRAC	2 cut(s)	16, 2107
HindII	GTYRAC	2 cut(s)	16, 2107
HindIII	AAGCTT	3 cut(s)	339, 557, 1892
HinfI	GANTC	8 cut(s)	747, 1192, 1221, 1391, 1818, 2119, 2206, 2230
HpaI	GTTAAC	1 cut(s)	16
HpaII	CCGG	1 cut(s)	1679
HphI	GGTGA	2 cut(s)	1507, 1830
Hpy166II	GTNNAC	4 cut(s)	16, 993, 2038, 2107
Hpy8I	GTNNAC	4 cut(s)	16, 993, 2038, 2107
Hpy99I	CGWCG	3 cut(s)	11, 14, 2252
HpyAV	CCTTC	3 cut(s)	337, 715, 1867
HpyCH4III	ACNGT	5 cut(s)	41, 1254, 1531, 2080, 2240
HpyCH4IV	ACGT	1 cut(s)	18
HpyF3I	CTNAG	8 cut(s)	141, 490, 823, 1757, 1926, 1970, 2005, 2208
HpySE526I	ACGT	1 cut(s)	18
Hsp92I	GRCGYC	1 cut(s)	6
Hsp92II	CATG	7 cut(s)	157, 228, 971, 1135, 1525, 1918, 2131
KflI	GGGWCCC	1 cut(s)	148
Ksp22I	TGATCA	1 cut(s)	1654
KspAI	GTTAAC	1 cut(s)	16
Kzo9I	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
LguI	GCTCTTC	2 cut(s)	519, 2034
LmnI	GCTCC	2 cut(s)	1238, 1679
Lsp1109I	GCAGC	8 cut(s)	182, 364, 871, 1329, 1414, 1705, 1708, 1992
LweI	GCATC	4 cut(s)	871, 1930, 1931, 2052
MaeI	CTAG	9 cut(s)	174, 212, 332, 690, 695, 902, 1233, 1416, 1484
MaeII	ACGT	1 cut(s)	18
MaeIII	GTNAC	1 cut(s)	1836
MalI	GATC	7 cut(s)	180, 681, 1031, 1275, 1317, 1609, 1656
MboI	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
MfeI	CAATTG	4 cut(s)	276, 1311, 1713, 2022
MflI	RGATCY	3 cut(s)	178, 679, 1273
MhlI	GDGCHC	1 cut(s)	321
MlsI	TGGCCA	1 cut(s)	1159
MluNI	TGGCCA	1 cut(s)	1159
MlyI	GAGTC	4 cut(s)	756, 1186, 2200, 2224
MmeI	TCCRAC	4 cut(s)	468, 921, 1786, 2198
Mox20I	TGGCCA	1 cut(s)	1159
MroXI	GAANNNNTTC	1 cut(s)	948
MscI	TGGCCA	1 cut(s)	1159
MseI	TTAA	8 cut(s)	15, 387, 974, 1377, 1563, 2067, 2090, 2160
MslI	CAYNNNNRTG	4 cut(s)	188, 223, 1416, 1520
Msp20I	TGGCCA	1 cut(s)	1159
MspA1I	CMGCKG	2 cut(s)	1550, 2273
MspCI	CTTAAG	2 cut(s)	973, 2066
MspI	CCGG	1 cut(s)	1679
MspR9I	CCNGG	4 cut(s)	459, 855, 1161, 1679
MunI	CAATTG	4 cut(s)	276, 1311, 1713, 2022
MvaI	CCWGG	3 cut(s)	459, 855, 1161
MvnI	CGCG	1 cut(s)	866
NciI	CCSGG	1 cut(s)	1679
NdeI	CATATG	2 cut(s)	1360, 1387
NdeII	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
NlaIII	CATG	7 cut(s)	157, 228, 971, 1135, 1525, 1918, 2131
NlaIV	GGNNCC	5 cut(s)	25, 149, 150, 935, 2218
NmuCI	GTSAC	1 cut(s)	1836
NspI	RCATGY	3 cut(s)	1525, 1918, 2131
NspV	TTCGAA	2 cut(s)	955, 1323
PaeI	GCATGC	2 cut(s)	1525, 2131
PceI	AGGCCT	1 cut(s)	875
PciSI	GCTCTTC	2 cut(s)	519, 2034
PcsI	WCGNNNNNNNCGW	2 cut(s)	15, 805
PdmI	GAANNNNTTC	1 cut(s)	948
PfeI	GAWTC	4 cut(s)	1221, 1391, 1818, 2119
PflMI	CCANNNNNTGG	1 cut(s)	1379
PleI	GAGTC	4 cut(s)	755, 1186, 2200, 2224
PpsI	GAGTC	4 cut(s)	755, 1186, 2200, 2224
PpuMI	RGGWCCY	1 cut(s)	148
PshBI	ATTAAT	1 cut(s)	2160
PsiI	TTATAA	1 cut(s)	2055
Psp124BI	GAGCTC	1 cut(s)	321
Psp1406I	AACGTT	1 cut(s)	18
Psp5II	RGGWCCY	1 cut(s)	148
Psp6I	CCWGG	3 cut(s)	457, 853, 1159
PspEI	GGTNACC	1 cut(s)	1836
PspFI	CCCAGC	2 cut(s)	248, 1976
PspGI	CCWGG	3 cut(s)	457, 853, 1159
PspN4I	GGNNCC	5 cut(s)	25, 149, 150, 935, 2218
PspPI	GGNCC	9 cut(s)	24, 148, 215, 324, 933, 1145, 1531, 1790, 2216
PspPPI	RGGWCCY	1 cut(s)	148
PstNI	CAGNNNCTG	1 cut(s)	377
PsuI	RGATCY	3 cut(s)	178, 679, 1273
PvuII	CAGCTG	2 cut(s)	1550, 2273
RsaI	GTAC	3 cut(s)	699, 778, 1992
RsaNI	GTAC	3 cut(s)	698, 777, 1991
RseI	CAYNNNNRTG	4 cut(s)	188, 223, 1416, 1520
SacI	GAGCTC	1 cut(s)	321
SapI	GCTCTTC	2 cut(s)	519, 2034
SaqAI	TTAA	8 cut(s)	15, 387, 974, 1377, 1563, 2067, 2090, 2160
Sau3AI	GATC	7 cut(s)	178, 679, 1029, 1273, 1315, 1607, 1654
Sau96I	GGNCC	9 cut(s)	24, 148, 215, 324, 933, 1145, 1531, 1790, 2216
SchI	GAGTC	4 cut(s)	756, 1186, 2200, 2224
ScrFI	CCNGG	4 cut(s)	459, 855, 1161, 1679
SduI	GDGCHC	1 cut(s)	321
SfaNI	GCATC	4 cut(s)	871, 1930, 1931, 2052
SfcI	CTRYAG	4 cut(s)	381, 633, 1551, 2100
SfuI	TTCGAA	2 cut(s)	955, 1323
SinI	GGWCC	5 cut(s)	148, 215, 1145, 1531, 2216
SmiMI	CAYNNNNRTG	4 cut(s)	188, 223, 1416, 1520
SmlI	CTYRAG	5 cut(s)	475, 551, 973, 2066, 2140
SmoI	CTYRAG	5 cut(s)	475, 551, 973, 2066, 2140
SpeI	ACTAGT	1 cut(s)	1483
SphI	GCATGC	2 cut(s)	1525, 2131
SseBI	AGGCCT	1 cut(s)	875
SsiI	CCGC	7 cut(s)	161, 290, 726, 866, 1236, 1506, 2047
SspI	AATATT	1 cut(s)	949
SspMI	CTAG	9 cut(s)	174, 212, 332, 690, 695, 902, 1233, 1416, 1484
SstI	GAGCTC	1 cut(s)	321
StuI	AGGCCT	1 cut(s)	875
StyD4I	CCNGG	4 cut(s)	457, 853, 1159, 1677
StyI	CCWWGG	2 cut(s)	1278, 1829
TaaI	ACNGT	5 cut(s)	41, 1254, 1531, 2080, 2240
TaiI	ACGT	1 cut(s)	21
TaqI	TCGA	8 cut(s)	46, 562, 955, 1323, 1338, 1897, 2060, 2250
TatI	WGTACW	1 cut(s)	697
TauI	GCSGC	2 cut(s)	164, 869
TfiI	GAWTC	4 cut(s)	1221, 1391, 1818, 2119
Tru1I	TTAA	8 cut(s)	15, 387, 974, 1377, 1563, 2067, 2090, 2160
Tru9I	TTAA	8 cut(s)	15, 387, 974, 1377, 1563, 2067, 2090, 2160
TscAI	CASTG	5 cut(s)	265, 900, 1000, 1155, 2232
TseFI	GTSAC	1 cut(s)	1836
TseI	GCWGC	8 cut(s)	170, 377, 884, 1342, 1402, 1718, 1721, 1980
Tsp45I	GTSAC	1 cut(s)	1836
TspDTI	ATGAA	9 cut(s)	525, 656, 785, 956, 1148, 1383, 1400, 1875, 2292
TspRI	CASTG	5 cut(s)	265, 900, 1000, 1155, 2232
Van91I	CCANNNNNTGG	1 cut(s)	1379
Vha464I	CTTAAG	2 cut(s)	973, 2066
VpaK11BI	GGWCC	5 cut(s)	148, 215, 1145, 1531, 2216
VspI	ATTAAT	1 cut(s)	2160
XapI	RAATTY	4 cut(s)	450, 984, 1407, 1643
XceI	RCATGY	3 cut(s)	1525, 1918, 2131
XmnI	GAANNNNTTC	1 cut(s)	948
XspI	CTAG	9 cut(s)	174, 212, 332, 690, 695, 902, 1233, 1416, 1484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.