Rroxscaffold_1G00048630

Glutamate-gated receptor that probably acts as non- selective cation channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010541
Physical Location & Seq
Reverse (-)
68678723 .. 68681412
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048630.1

Sequence Viewer

Length: 2100 bp
ATGCAAGAAGTTCAAGCCATTCTAGGACCACATACATGGGAAGAGACATCAGTAGTAGCTGGGGTTGGCAGTGAGAACCGCATTCCAATTGTTTCTTTTGCGGAGGCTACTCCAAACTGGGCAACAGAGCTCTGGCCCTTCCTAGTTCAAGCTTCTCATAATGAGCTAAAACAAATGGAAGCAGTAGCTGCTATAGTTAAGTCTTTTGAATGGTATCAGGTCACTGTTATATACCAAGATAGAGATTATTCGTCCAACGAAATTTTGCCTGGCCTCTCTCGTGCCTTGAGAGAAGTTGGCTCAGAAATAAGCCAGCTCGTAGCTCTTCCCCCTTTAGGTTCTTCTTCATTAGTCAGAGAGCTTGAGAAGCTTCGACAAGACCAATATAGAGTTGTCATCGTGCATTTGTCTTTGCCGTTGGCATTGCAACTATTTGAAAAAGCTAAAGAAATGAAGATGATGGAAAAAGACTATGTATGGATCACCACAGATCCTATTACTAGCCTAGTACATTCCTTCAATGCCTCCACCATTTCCGCAATGCAGGGGGTTATCGGAGTCAAGAGCTTCTTCCCTGAAAGTGAGAGTCCCCAATTTCAGGGTTTTCATCGTAGATTTCGTAGAAGGTTCAGCTCAGAATATCCTGAAGAAGAAAACAATGATCCTGGGGTCTTCGCGGCACAGGCCTATGATGCTGCAAGGGCAGTGGTTCTAGCAGTGACACAAAGAAAACACAATGGGAGGCCCTTGTTGGAAAATATTCTTCGAAGTGATTTTCATGGCTTAGGTGGAAAAATTCAGTTCACTGACCAAAAATTACCAATACAAAAAATGTTCCAGATCATCAATGTTATAGGAAAGAGTTACAGAGAACTCGGATATTGGGGGACTTTCTATACTCCAAAAGGATGGACCGTATCAACAAGTTCAAAGCCATTGAGAATCGGAGTGCCTACCGGAGCGGCATTCAAACAGTATGCAAATGTAGAAAAAGATCCCTTGGGTAATAACTTCTCATTTGGTGGAATTGCAATTGATCTCTTCGAAGAAACCCTTATCGAGCTGCCTTATCATTTACCATATGAATTCTTCCCATTTAATGGCACATATGATTCATTAGTGCAGCAAATTCATTCTAGTCAGAAATTTGATGCTGTAGTTGGTGATGTAGCAATAGTGTCGCAAAGATATCAGTATGCAGAATTCACACACCCCTATTCTGAAGCAGGACTGATGATGATAGTTCCAGTCAGGTCCAAATTTTGCAACAAAGCATGGTTATTCATGAACCCTTTCACACGAGCCATGTGGACGCTCATAGTGGCCATCAATGTCTACAATGGTTTTGTCGTTTGGTTGATAGAGCGAAACTACTGCCCCGAACTCAGAGGTTCTCTGCCAAATCAAATTGGAACCTTGATTTGGCTTTCCTTCACCACTCTATTCTCCTTACATGTCATCACACAGACATACACCGCCAACCTCACCAGCATGCTTACGGTCCAGCAACTTGAACCAGCTGTAGCAGATGTTGATGCACTTCTACAAAGCAATGCCGTTGTTGGTTATTGTAAAGGATCATTTGTTTCTATGTATCTAAAGAAAGTCTTGAAATTTTCCCCTGATCACATTCAGGAGTTTAGCTCCCCGGAAGAGTATGCAAAAGCCCTCAAAAGTAAAGCAATTGCTGCTGCATTTCTTGAAGCATTTCCAAGGGGGTCACCATTACTTCCGAGTGTAAATGAAGCAATGCTGAAGGTGGCCGAAAGTGGTAAGCTTCGAGATTTGGAGAACAACATGCTGGCATCTCAGAAATGCTTGGATGCAGAAACGGATGGTAGAACCCCAAGTCTAAGCCCCAGCAGCTTTTGGGTACTATTCACATTCTCAGGAGGGACATCAACAATTGCTCTTCTGGTTTACATTTTCCGCATTTATAAATCGATGCTTAAGCACAAAACAGTCTGGAACTTAATGTTGGCTGTAGTCAACCAATGGGGGAATCCAAGTATGCAGAGGTTCTCAAGAAGAGACAGCGACATTAATCCAGAAACTCCGACGCATTCTGCAAATGTCCCTAACTTGCAGGCTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

699

Amino Acids

78.75

Weight (kDa)

6.56

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 2 - 286 1.8e-46 Receptor family ligand binding region
Peripla_BP_6 PF13458 2 - 278 7.4e-18 Periplasmic binding protein
SBP_bac_3 PF00497 339 - 598 1.1e-07 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 482 - 629 6.6e-08 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1938
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccBSI CCGCTC 1 cut(s) 962
AccI GTMKAC 1 cut(s) 1335
AccII CGCG 1 cut(s) 677
AciI CCGC 7 cut(s) 79, 101, 537, 677, 962, 1476, 1930
AclWI GGATC 5 cut(s) 485, 488, 656, 989, 1585
AcoI YGGCCR 2 cut(s) 1323, 1761
AcsI RAATTY 8 cut(s) 261, 795, 1085, 1128, 1145, 1202, 1259, 1613
AcuI CTGAAG 3 cut(s) 666, 1242, 1775
AfaI GTAC 2 cut(s) 510, 1875
AfiI CCNNNNNNNGG 4 cut(s) 335, 598, 1100, 1422
AflII CTTAAG 1 cut(s) 1949
AflIII ACRYGT 1 cut(s) 1453
AjnI CCWGG 2 cut(s) 268, 664
AjuI GAANNNNNNNTTGG 4 cut(s) 1405, 1437, 1961, 1993
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 38, 2025
AlwI GGATC 5 cut(s) 485, 488, 656, 989, 1585
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 6 cut(s) 134, 271, 684, 743, 1323, 1761
ApeKI GCWGC 7 cut(s) 188, 695, 1063, 1123, 1688, 1691, 1863
ApoI RAATTY 8 cut(s) 261, 795, 1085, 1128, 1145, 1202, 1259, 1613
AseI ATTAAT 1 cut(s) 2043
Asp700I GAANNNNTTC 3 cut(s) 759, 1292, 1707
AspS9I GGNCC 6 cut(s) 26, 135, 744, 912, 1254, 1501
AsuC2I CCSGG 1 cut(s) 1649
AsuHPI GGTGA 5 cut(s) 475, 1175, 1426, 1477, 1713
AsuII TTCGAA 2 cut(s) 766, 1044
AvaII GGWCC 4 cut(s) 26, 912, 1254, 1501
BalI TGGCCA 1 cut(s) 1325
BanII GRGCYC 1 cut(s) 132
BauI CACGAG 2 cut(s) 279, 1299
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 7 cut(s) 175, 682, 1050, 1135, 1675, 1678, 1875
BccI CCATC 4 cut(s) 454, 903, 1334, 1829
BceAI ACGGC 2 cut(s) 400, 1541
BcgI CGANNNNNNTGC 4 cut(s) 1161, 1195, 1356, 1390
BciT130I CCWGG 2 cut(s) 270, 666
BclI TGATCA 1 cut(s) 1624
BcnI CCSGG 1 cut(s) 1649
BcoDI GTCTC 2 cut(s) 38, 2025
BfaI CTAG 7 cut(s) 23, 143, 501, 506, 713, 1137, 2098
BfmI CTRYAG 4 cut(s) 192, 1155, 1521, 1983
BfrI CTTAAG 1 cut(s) 1949
BisI GCNGC 9 cut(s) 189, 678, 696, 963, 1064, 1124, 1689, 1692, 1864
BlsI GCNGC 9 cut(s) 190, 679, 697, 964, 1065, 1125, 1690, 1693, 1865
Bme1390I CCNGG 3 cut(s) 270, 666, 1649
Bme18I GGWCC 4 cut(s) 26, 912, 1254, 1501
BmgT120I GGNCC 6 cut(s) 26, 135, 744, 912, 1254, 1501
BmiI GGNNCC 1 cut(s) 1414
BmrFI CCNGG 3 cut(s) 270, 666, 1649
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 6 cut(s) 682, 1141, 1525, 1813, 1814, 1935
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 664
BplI GAGNNNNNCTC 2 cut(s) 1628, 1660
Bpu10I CCTNAGC 2 cut(s) 784, 2091
Bpu14I TTCGAA 2 cut(s) 766, 1044
BpuEI CTTGAG 3 cut(s) 307, 383, 2008
BpuMI CCSGG 1 cut(s) 1649
Bsa29I ATCGAT 1 cut(s) 1943
BsaJI CCNNGG 4 cut(s) 665, 999, 1647, 1712
BsaWI WCCGGW 1 cut(s) 956
Bsc4I CCNNNNNNNGG 4 cut(s) 335, 598, 1100, 1422
Bse1I ACTGG 2 cut(s) 122, 1247
Bse3DI GCAATG 4 cut(s) 422, 546, 1558, 1755
BseBI CCWGG 2 cut(s) 270, 666
BseCI ATCGAT 1 cut(s) 1943
BseDI CCNNGG 4 cut(s) 665, 999, 1647, 1712
BseGI GGATG 3 cut(s) 914, 1828, 1840
BseLI CCNNNNNNNGG 4 cut(s) 335, 598, 1100, 1422
BseMI GCAATG 4 cut(s) 422, 546, 1558, 1755
BseMII CTCAG 5 cut(s) 315, 648, 1399, 1823, 1902
BseNI ACTGG 2 cut(s) 122, 1247
BseXI GCAGC 7 cut(s) 175, 682, 1050, 1135, 1675, 1678, 1875
BseYI CCCAGC 2 cut(s) 59, 1859
BsgI GTGCAG 1 cut(s) 1142
Bsh1236I CGCG 1 cut(s) 677
BshFI GGCC 6 cut(s) 136, 273, 686, 745, 1325, 1763
BshVI ATCGAT 1 cut(s) 1943
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 2 cut(s) 957, 1649
BslFI GGGAC 4 cut(s) 573, 901, 1909, 2060
BslI CCNNNNNNNGG 4 cut(s) 335, 598, 1100, 1422
BsmAI GTCTC 2 cut(s) 38, 2025
BsmFI GGGAC 4 cut(s) 573, 901, 1909, 2060
BsmI GAATGC 3 cut(s) 81, 965, 2062
BsnI GGCC 6 cut(s) 136, 273, 686, 745, 1325, 1763
Bsp119I TTCGAA 2 cut(s) 766, 1044
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
BspACI CCGC 7 cut(s) 79, 101, 537, 677, 962, 1476, 1930
BspANI GGCC 6 cut(s) 136, 273, 686, 745, 1325, 1763
BspCNI CTCAG 5 cut(s) 314, 647, 1398, 1822, 1901
BspDI ATCGAT 1 cut(s) 1943
BspFNI CGCG 1 cut(s) 677
BspHI TCATGA 1 cut(s) 1284
BspLI GGNNCC 1 cut(s) 1414
BspPI GGATC 5 cut(s) 485, 488, 656, 989, 1585
BspQI GCTCTTC 2 cut(s) 330, 1917
BspT104I TTCGAA 2 cut(s) 766, 1044
BspTI CTTAAG 1 cut(s) 1949
BsrBI CCGCTC 1 cut(s) 962
BsrDI GCAATG 4 cut(s) 422, 546, 1558, 1755
BsrI ACTGG 2 cut(s) 122, 1247
BssECI CCNNGG 4 cut(s) 665, 999, 1647, 1712
BssMI GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
BssSI CACGAG 2 cut(s) 279, 1299
BssT1I CCWWGG 2 cut(s) 999, 1712
Bst2BI CACGAG 2 cut(s) 279, 1299
Bst2UI CCWGG 2 cut(s) 270, 666
Bst4CI ACNGT 5 cut(s) 226, 916, 975, 1501, 1963
Bst6I CTCTTC 6 cut(s) 36, 330, 1046, 1647, 1917, 2023
BstAFI CTTAAG 1 cut(s) 1949
BstAPI GCANNNNNTGC 2 cut(s) 188, 1688
BstBI TTCGAA 2 cut(s) 766, 1044
BstC8I GCNNGC 4 cut(s) 314, 1493, 1803, 2088
BstDEI CTNAG 8 cut(s) 301, 634, 784, 1385, 1809, 1853, 1888, 2091
BstEII GGTNACC 1 cut(s) 1719
BstF5I GGATG 3 cut(s) 914, 1828, 1840
BstFNI CGCG 1 cut(s) 677
BstKTI GATC 8 cut(s) 483, 493, 664, 843, 997, 1039, 1580, 1627
BstMAI GTCTC 2 cut(s) 38, 2025
BstMBI GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
BstMWI GCNNNNNNNGC 7 cut(s) 188, 367, 683, 692, 701, 1688, 1863
BstNI CCWGG 2 cut(s) 270, 666
BstNSI RCATGY 3 cut(s) 1457, 1495, 1801
BstPI GGTNACC 1 cut(s) 1719
BstSCI CCNGG 3 cut(s) 268, 664, 1647
BstSFI CTRYAG 4 cut(s) 192, 1155, 1521, 1983
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 7 cut(s) 175, 682, 1050, 1135, 1675, 1678, 1875
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 490, 994
BstXI CCANNNNNNTGG 2 cut(s) 36, 909
BstYI RGATCY 2 cut(s) 490, 994
Bsu15I ATCGAT 1 cut(s) 1943
BsuRI GGCC 6 cut(s) 136, 273, 686, 745, 1325, 1763
BsuTUI ATCGAT 1 cut(s) 1943
BtsCI GGATG 3 cut(s) 914, 1828, 1840
BtsI GCAGTG 3 cut(s) 76, 711, 723
BtsIMutI CAGTG 5 cut(s) 76, 222, 711, 723, 804
Cac8I GCNNGC 4 cut(s) 314, 1493, 1803, 2088
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 1284
Cfr13I GGNCC 6 cut(s) 26, 135, 744, 912, 1254, 1501
ClaI ATCGAT 1 cut(s) 1943
CseI GACGC 2 cut(s) 1321, 2068
Csp6I GTAC 2 cut(s) 509, 1874
CspCI CAANNNNNGTGG 2 cut(s) 687, 722
CviAII CATG 8 cut(s) 36, 779, 1275, 1285, 1306, 1454, 1492, 1798
CviQI GTAC 2 cut(s) 509, 1874
DdeI CTNAG 8 cut(s) 301, 634, 784, 1385, 1809, 1853, 1888, 2091
DpnI GATC 8 cut(s) 482, 492, 663, 842, 996, 1038, 1579, 1626
DpnII GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
EaeI YGGCCR 2 cut(s) 1323, 1761
Eam1104I CTCTTC 6 cut(s) 36, 330, 1046, 1647, 1917, 2023
EarI CTCTTC 6 cut(s) 36, 330, 1046, 1647, 1917, 2023
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 999, 1712
Eco147I AGGCCT 1 cut(s) 686
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 1190
Eco47I GGWCC 4 cut(s) 26, 912, 1254, 1501
Eco53kI GAGCTC 1 cut(s) 130
Eco57I CTGAAG 3 cut(s) 666, 1242, 1775
Eco91I GGTNACC 1 cut(s) 1719
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 744
EcoO65I GGTNACC 1 cut(s) 1719
EcoRI GAATTC 2 cut(s) 1085, 1202
EcoRII CCWGG 2 cut(s) 268, 664
EcoRV GATATC 1 cut(s) 1190
EcoT14I CCWWGG 2 cut(s) 999, 1712
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 999, 1712
FaeI CATG 8 cut(s) 39, 782, 1278, 1288, 1309, 1457, 1495, 1801
FalI AAGNNNNNCTT 6 cut(s) 554, 586, 1038, 1070, 1592, 1624
FaqI GGGAC 4 cut(s) 573, 901, 1909, 2060
FatI CATG 8 cut(s) 35, 778, 1274, 1284, 1305, 1453, 1491, 1797
FauNDI CATATG 2 cut(s) 1081, 1108
FbaI TGATCA 1 cut(s) 1624
FblI GTMKAC 1 cut(s) 1335
Fnu4HI GCNGC 9 cut(s) 189, 678, 696, 963, 1064, 1124, 1689, 1692, 1864
FokI GGATG 3 cut(s) 921, 1835, 1847
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 9 cut(s) 189, 678, 696, 963, 1064, 1124, 1689, 1692, 1864
FspBI CTAG 7 cut(s) 23, 143, 501, 506, 713, 1137, 2098
GluI GCNGC 9 cut(s) 189, 678, 696, 963, 1064, 1124, 1689, 1692, 1864
GsaI CCCAGC 2 cut(s) 63, 1863
HaeIII GGCC 6 cut(s) 136, 273, 686, 745, 1325, 1763
HapII CCGG 2 cut(s) 957, 1649
HgaI GACGC 2 cut(s) 1321, 2068
Hin1II CATG 8 cut(s) 39, 782, 1278, 1288, 1309, 1457, 1495, 1801
HincII GTYRAC 1 cut(s) 1990
HindII GTYRAC 1 cut(s) 1990
HindIII AAGCTT 3 cut(s) 150, 368, 1775
HinfI GANTC 5 cut(s) 558, 586, 942, 1112, 2002
HpaII CCGG 2 cut(s) 957, 1649
HphI GGTGA 5 cut(s) 475, 1175, 1426, 1477, 1713
Hpy166II GTNNAC 5 cut(s) 804, 1311, 1336, 1921, 1990
Hpy8I GTNNAC 5 cut(s) 804, 1311, 1336, 1921, 1990
Hpy99I CGWCG 1 cut(s) 2062
HpyAV CCTTC 5 cut(s) 148, 526, 618, 1441, 1750
HpyCH4III ACNGT 5 cut(s) 226, 916, 975, 1501, 1963
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 367, 683, 692, 701, 1688, 1863
HpyF3I CTNAG 8 cut(s) 301, 634, 784, 1385, 1809, 1853, 1888, 2091
Hsp92II CATG 8 cut(s) 39, 782, 1278, 1288, 1309, 1457, 1495, 1801
Ksp22I TGATCA 1 cut(s) 1624
Kzo9I GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
LguI GCTCTTC 2 cut(s) 330, 1917
LmnI GCTCC 2 cut(s) 959, 1649
Lsp1109I GCAGC 7 cut(s) 175, 682, 1050, 1135, 1675, 1678, 1875
LweI GCATC 6 cut(s) 682, 1141, 1525, 1813, 1814, 1935
MaeI CTAG 7 cut(s) 23, 143, 501, 506, 713, 1137, 2098
MaeIII GTNAC 4 cut(s) 220, 718, 863, 1719
MalI GATC 8 cut(s) 482, 492, 663, 842, 996, 1038, 1579, 1626
MbiI CCGCTC 1 cut(s) 962
MboI GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
MfeI CAATTG 4 cut(s) 87, 1032, 1683, 1905
MflI RGATCY 2 cut(s) 490, 994
MhlI GDGCHC 1 cut(s) 132
MlsI TGGCCA 1 cut(s) 1325
MluNI TGGCCA 1 cut(s) 1325
MlyI GAGTC 2 cut(s) 567, 595
MmeI TCCRAC 3 cut(s) 279, 732, 2081
MnlI CCTC 9 cut(s) 97, 284, 535, 735, 1382, 1493, 1679, 1886, 2010
Mox20I TGGCCA 1 cut(s) 1325
MroXI GAANNNNTTC 3 cut(s) 759, 1292, 1707
MscI TGGCCA 1 cut(s) 1325
MseI TTAA 5 cut(s) 198, 1098, 1950, 1973, 2043
MslI CAYNNNNRTG 2 cut(s) 34, 1490
Msp20I TGGCCA 1 cut(s) 1325
MspA1I CMGCKG 1 cut(s) 1520
MspCI CTTAAG 1 cut(s) 1949
MspI CCGG 2 cut(s) 957, 1649
MspR9I CCNGG 3 cut(s) 270, 666, 1649
MunI CAATTG 4 cut(s) 87, 1032, 1683, 1905
Mva1269I GAATGC 3 cut(s) 81, 965, 2062
MvaI CCWGG 2 cut(s) 270, 666
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 7 cut(s) 188, 367, 683, 692, 701, 1688, 1863
NciI CCSGG 1 cut(s) 1649
NdeI CATATG 2 cut(s) 1081, 1108
NdeII GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
NlaIII CATG 8 cut(s) 39, 782, 1278, 1288, 1309, 1457, 1495, 1801
NlaIV GGNNCC 1 cut(s) 1414
NmuCI GTSAC 3 cut(s) 220, 718, 1719
NspI RCATGY 3 cut(s) 1457, 1495, 1801
NspV TTCGAA 2 cut(s) 766, 1044
PaeI GCATGC 1 cut(s) 1495
PagI TCATGA 1 cut(s) 1284
PceI AGGCCT 1 cut(s) 686
PciI ACATGT 1 cut(s) 1453
PciSI GCTCTTC 2 cut(s) 330, 1917
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 3 cut(s) 81, 965, 2062
PdmI GAANNNNTTC 3 cut(s) 759, 1292, 1707
PfeI GAWTC 3 cut(s) 942, 1112, 2002
PflMI CCANNNNNTGG 1 cut(s) 1100
PkrI GCNGC 9 cut(s) 190, 679, 697, 964, 1065, 1125, 1690, 1693, 1865
PleI GAGTC 2 cut(s) 566, 594
PpsI GAGTC 2 cut(s) 566, 594
PscI ACATGT 1 cut(s) 1453
PshBI ATTAAT 1 cut(s) 2043
PsiI TTATAA 1 cut(s) 1938
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 2 cut(s) 268, 664
PspEI GGTNACC 1 cut(s) 1719
PspFI CCCAGC 2 cut(s) 59, 1859
PspGI CCWGG 2 cut(s) 268, 664
PspN4I GGNNCC 1 cut(s) 1414
PspPI GGNCC 6 cut(s) 26, 135, 744, 912, 1254, 1501
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 490, 994
PvuII CAGCTG 1 cut(s) 1520
RsaI GTAC 2 cut(s) 510, 1875
RsaNI GTAC 2 cut(s) 509, 1874
RseI CAYNNNNRTG 2 cut(s) 34, 1490
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 2 cut(s) 330, 1917
SaqAI TTAA 5 cut(s) 198, 1098, 1950, 1973, 2043
SatI GCNGC 9 cut(s) 189, 678, 696, 963, 1064, 1124, 1689, 1692, 1864
Sau3AI GATC 8 cut(s) 480, 490, 661, 840, 994, 1036, 1577, 1624
Sau96I GGNCC 6 cut(s) 26, 135, 744, 912, 1254, 1501
SchI GAGTC 2 cut(s) 567, 595
ScrFI CCNGG 3 cut(s) 270, 666, 1649
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 6 cut(s) 682, 1141, 1525, 1813, 1814, 1935
SfcI CTRYAG 4 cut(s) 192, 1155, 1521, 1983
SfuI TTCGAA 2 cut(s) 766, 1044
SinI GGWCC 4 cut(s) 26, 912, 1254, 1501
SmiMI CAYNNNNRTG 2 cut(s) 34, 1490
SmlI CTYRAG 4 cut(s) 286, 362, 1949, 2023
SmoI CTYRAG 4 cut(s) 286, 362, 1949, 2023
SphI GCATGC 1 cut(s) 1495
SseBI AGGCCT 1 cut(s) 686
SsiI CCGC 7 cut(s) 79, 101, 537, 677, 962, 1476, 1930
SspI AATATT 1 cut(s) 760
SspMI CTAG 7 cut(s) 23, 143, 501, 506, 713, 1137, 2098
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 686
StyD4I CCNGG 3 cut(s) 268, 664, 1647
StyI CCWWGG 2 cut(s) 999, 1712
TaaI ACNGT 5 cut(s) 226, 916, 975, 1501, 1963
TaqI TCGA 6 cut(s) 373, 766, 1044, 1059, 1780, 1943
TatI WGTACW 1 cut(s) 508
TauI GCSGC 2 cut(s) 680, 965
TfiI GAWTC 3 cut(s) 942, 1112, 2002
Tru1I TTAA 5 cut(s) 198, 1098, 1950, 1973, 2043
Tru9I TTAA 5 cut(s) 198, 1098, 1950, 1973, 2043
TscAI CASTG 5 cut(s) 76, 229, 711, 723, 811
TseFI GTSAC 3 cut(s) 220, 718, 1719
TseI GCWGC 7 cut(s) 188, 695, 1063, 1123, 1688, 1691, 1863
Tsp45I GTSAC 3 cut(s) 220, 718, 1719
TspGWI ACGGA 1 cut(s) 1847
TspRI CASTG 5 cut(s) 76, 229, 711, 723, 811
Van91I CCANNNNNTGG 1 cut(s) 1100
Vha464I CTTAAG 1 cut(s) 1949
VpaK11BI GGWCC 4 cut(s) 26, 912, 1254, 1501
VspI ATTAAT 1 cut(s) 2043
XapI RAATTY 8 cut(s) 261, 795, 1085, 1128, 1145, 1202, 1259, 1613
XceI RCATGY 3 cut(s) 1457, 1495, 1801
XmiI GTMKAC 1 cut(s) 1335
XmnI GAANNNNTTC 3 cut(s) 759, 1292, 1707
XspI CTAG 7 cut(s) 23, 143, 501, 506, 713, 1137, 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.