FvH4_3g19920

spa1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000964
Physical Location & Seq
Reverse (-)
13058649 .. 13069087
10439 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19920.t5

Sequence Viewer

Length: 2301 bp
ATGTGTGTGTTCTTGCCTTCATGCTTCTATAGGAGGATAACAATGGAAGGCTCATCTGAGAATGCTTGTCAGAGGTCTAATAGTTCTAGAAATTTGAATGCTTCCAGAGTCTCAAACCGGAATCCAAGGCTTAATTATGCTAGAAGATTCGGCTTCTTAGGTGGTTCATCGCAGGATTCAGATTTAAGGAATGATAGAGATAGGGTTTTGGTGGCACACACTGATGACCTAGGAAACCAGGGTGGATTGTCTGGGGTGTGTGAGGATGAGGTGCCAGTTGAATCTTTTGTCCGAGCTATTGAATGGGGGGATGTTAGCTTGAGGCAATGGTTGGATAGACCGGATCGAGCTGTTGATGCATTTGAATGCTTGCATATATTTAGGCAAATAGTAGAAATTGTTAATGTAGCACATTCAGAAGGAATCGTTGTTCATAATGTGCGGCCTTCCTGCTTTGTTATGTCCTCTTTTAATCGTGTCTCTTTCATCGAGTCTGCATCTTGCTCAGATTCTGGCACTGATTCTCCCGAAGATGGGTTAACTAGCCCAGCCCTGGAAGCTAAGAAGTTGACCTCTGCCTTGCATCAGAAACGAAGCAACGTAGCAAGTGGGAATTTTCGATTCATGAAGGCTCCAGCAAATGCTTTGTCAGATACAAGTTGCATGCAATCAAGCTCAATATATGCAGCACGTGAATCTTTAATGCAAGAGAGTGAAGAACATAGAACCAGAGAGAGAAGTGCTCAACTAGAGGATAAGAGACAGCCATTTCCAATGAAACAAATATTGCTCATGGAGAGCAATTGGTATACTAGTCCTGAAGAGGTTGCTGGTGGTCCAAGTCCCTGTGCTTCAGACATTTACCGATTAGGAGTTCTTCTTTTTGAGTTATTCTGCCCATTCAGCTCAAGAGAAGAAAAAAGTAGGACTATGTCTAGTCTGAGACATCGAGTTCTTCCTCCACAGTTACTGCTGAGATGGCCGAAAGAAGCTTCATTTTGCTTATGGCTACTACACCCTGAGCCAAATAGTCGGCCTAAAATGGGTGAGTTGCTACAGAGTGAATTTCTTAATGAACCAAGAGATGATTTAGAAGAACGTGAAGCAGCAATAGAGCTTAGAGAGAAAATAGAAGAGCAGGAGTTATTGCTGGAATTTCTCATGCTTGTAAAACAAAGGAAGCAGGAAGCAGCAGATAAGTTACAAAACACTGTTTCATTTCTGTGTTCCGATATTGAAGAAGTTGTGAAGCACAAAACAAGTTCCAAGGGAAAAGGTGGTTCATGCCCAGATTTGGTAAAGGAAGATCATTCAACATCAAGTTTCCCATCTATGAATATTACTGATGATGATGATTCGGCTAGTGGATCCCGAAAACGGTTCAGACCAGGTGTCCAGATTCAGAATGGGGAGGAGTGTGATGACAATTTGGATGGTCAGAAGTCAGAAACTGATAACCAAGAAAGTATTCTTTTGAGAAGTTCCAGATTGATGAACAATTTTAAAAAGCTTGAGTCGGCATATTTTTTGACGAGATGGAGGCCAGTCAAGCACTCAACCAGGCCACTGACTAGGCATTCATCAATCAGTAGTGATGGTAGAGGTTCTATTATTGCAACTGAAAGAAGTTCTGTGGATAATTTGACATCAAAAGAACAACTTAGTGAAGGTAGACGAAGTGGTTGGATAGAACCATTCCTCGAGGGATTATGCAAGTATCTATCTTTCTCAAAATTAAAAGTTAAGGCGGATTTAAAGCAGGCTGATCTTCTAAATTCCTCAAATTTAGTATGCTCACTTAGTTTTGATCGCGATGGAGAATTTTTTGCTACAGCTGGTGTTAACAAGAAAATCAAAATATTTGAATGTGACTCAATCATAAATGAGGATCGTGACATCCACTATCCTGTGGTTGAAATAGCTACCAGATCAAAGCTAAGCAATATATGTTGGAACAGTTATATCAAAAGCCAGATTGCTTCAAGTAACTTTGAAGGTGTGGTGCAGGTGTGGGATGTTACAAGAAGTCAAGTACTAATGGAGATGAAAGAACATGAGAAGCGTGTATGGTCCATTGACTTTTCATCAGCAGACCCGACAATGCTGGCCAGCGGGAGCGACGATGGATCTGTTAAACTATGGAGTATCAATCAGGCAATTTTATTCTTGCACTTGGTGGATGTCAGCTTTGAAACTAAACGGAGAAAGTATTGGTACAATAAAAACAAAGGCCAATGTTTGCTGTGTACAGTTTCCTTTGGAGTCTTGTCGCTCACTTGCATTCGGTTCAGCGGATCATAA

Protein Analysis

767

Amino Acids

86.65

Weight (kDa)

6.11

Isoelectric Point (pI)

66.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 588 - 730 1.8e-13 WDR3 first beta-propeller domain
Beta-prop_WDR19_1st PF23389 590 - 736 5.7e-06 WDR19 first beta-propeller
Beta-prop_WDR5 PF25175 594 - 847 2e-31 WDR5 beta-propeller domain
WD40_Gbeta PF25391 594 - 762 5e-06 G protein beta WD-40 repeat protein
WDR55 PF24796 594 - 720 7.3e-07 WDR55
WD40_CDC20-Fz PF24807 643 - 736 1.1e-13 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 644 - 843 4.8e-24 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 657 - 729 5.8e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 657 - 805 6.6e-14 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 661 - 736 5e-10 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 663 - 798 1.6e-15 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 664 - 803 4e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 667 - 846 7.9e-26 WDR3 first beta-propeller domain
WD40 PF00400 678 - 715 1.7e-07 WD domain, G-beta repeat
EIF3I PF24805 683 - 739 8.7e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 683 - 837 1.2e-09 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 696 - 816 4.3e-10 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 696 - 847 2.6e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EIPR1 PF23609 722 - 798 7.9e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 726 - 805 3.9e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 727 - 838 1.7e-08 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 728 - 819 2.1e-07 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 742 - 837 1.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 742 - 902 7.6e-10 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1996
AbsI CCTCGAGG 1 cut(s) 1700
Acc36I ACCTGC 1 cut(s) 1996
AccB1I GGYRCC 1 cut(s) 271
AccI GTMKAC 2 cut(s) 809, 1672
AccII CGCG 1 cut(s) 1812
AciI CCGC 4 cut(s) 442, 1748, 2112, 2292
AclWI GGATC 5 cut(s) 351, 1362, 1375, 1896, 2134
AcoI YGGCCR 2 cut(s) 980, 2106
AcsI RAATTY 7 cut(s) 91, 613, 1064, 1154, 1774, 1783, 1820
AcuI CTGAAG 2 cut(s) 837, 840
AcvI CACGTG 1 cut(s) 692
AdeI CACNNNGTG 1 cut(s) 2176
AfaI GTAC 3 cut(s) 2034, 2216, 2248
AfiI CCNNNNNNNGG 6 cut(s) 533, 534, 553, 1043, 1294, 1377
AhdI GACNNNNNGTC 1 cut(s) 1391
AhlI ACTAGT 1 cut(s) 812
AjnI CCWGG 4 cut(s) 237, 552, 1387, 1559
AjuI GAANNNNNNNTTGG 2 cut(s) 1452, 1484
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 4 cut(s) 115, 484, 754, 937
AlwI GGATC 5 cut(s) 351, 1362, 1375, 1896, 2134
AlwNI CAGNNNCTG 3 cut(s) 512, 970, 1451
Ama87I CYCGRG 1 cut(s) 1700
AoxI GGCC 7 cut(s) 443, 980, 1034, 1541, 1562, 2106, 2230
ApeKI GCWGC 3 cut(s) 686, 1106, 1190
ApoI RAATTY 7 cut(s) 91, 613, 1064, 1154, 1774, 1783, 1820
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
Asp700I GAANNNNTTC 1 cut(s) 1467
AspA2I CCTAGG 1 cut(s) 229
AspS9I GGNCC 2 cut(s) 836, 2070
AsuHPI GGTGA 1 cut(s) 1058
AvaI CYCGRG 1 cut(s) 1700
AvaII GGWCC 2 cut(s) 836, 2070
AvrII CCTAGG 1 cut(s) 229
BalI TGGCCA 1 cut(s) 2108
BamHI GGATCC 1 cut(s) 1367
BanI GGYRCC 1 cut(s) 271
BbrPI CACGTG 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 745
BbvI GCAGC 3 cut(s) 698, 1118, 1202
BccI CCATC 8 cut(s) 527, 972, 1336, 1427, 1530, 1589, 1808, 2117
BciT130I CCWGG 4 cut(s) 239, 554, 1389, 1561
BcoDI GTCTC 4 cut(s) 115, 484, 754, 937
BcuI ACTAGT 1 cut(s) 812
BfaI CTAG 9 cut(s) 87, 141, 230, 543, 749, 813, 936, 1362, 1572
BfmI CTRYAG 3 cut(s) 28, 1055, 1830
BfuAI ACCTGC 1 cut(s) 1996
BisI GCNGC 4 cut(s) 443, 687, 1107, 1191
BlnI CCTAGG 1 cut(s) 229
BlpI GCTNAGC 1 cut(s) 1937
BlsI GCNGC 4 cut(s) 444, 688, 1108, 1192
BmcAI AGTACT 1 cut(s) 2034
Bme1390I CCNGG 4 cut(s) 239, 554, 1389, 1561
Bme18I GGWCC 2 cut(s) 836, 2070
BmeRI GACNNNNNGTC 1 cut(s) 1391
BmeT110I CYCGRG 1 cut(s) 1700
BmgT120I GGNCC 2 cut(s) 836, 2070
BmiI GGNNCC 3 cut(s) 273, 633, 1369
BmrFI CCNGG 4 cut(s) 239, 554, 1389, 1561
BmsI GCATC 3 cut(s) 346, 506, 592
BplI GAGNNNNNCTC 2 cut(s) 727, 759
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 1020
Bpu1102I GCTNAGC 1 cut(s) 1937
BpuEI CTTGAG 3 cut(s) 340, 892, 1532
BsaAI YACGTR 1 cut(s) 692
BsaJI CCNNGG 5 cut(s) 125, 229, 238, 552, 1266
BsaWI WCCGGW 2 cut(s) 117, 340
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 6 cut(s) 533, 534, 553, 1043, 1294, 1377
Bse1I ACTGG 2 cut(s) 275, 1544
Bse3DI GCAATG 1 cut(s) 332
BseBI CCWGG 4 cut(s) 239, 554, 1389, 1561
BseDI CCNNGG 5 cut(s) 125, 229, 238, 552, 1266
BseGI GGATG 6 cut(s) 271, 316, 1438, 1896, 2020, 2185
BseLI CCNNNNNNNGG 6 cut(s) 533, 534, 553, 1043, 1294, 1377
BseMI GCAATG 1 cut(s) 332
BseMII CTCAG 5 cut(s) 48, 519, 932, 965, 1011
BseNI ACTGG 2 cut(s) 275, 1544
BseRI GAGGAG 1 cut(s) 1427
BseXI GCAGC 3 cut(s) 698, 1118, 1202
BseYI CCCAGC 1 cut(s) 547
BsgI GTGCAG 1 cut(s) 2024
Bsh1236I CGCG 1 cut(s) 1812
BshFI GGCC 7 cut(s) 445, 982, 1036, 1543, 1564, 2108, 2232
BshNI GGYRCC 1 cut(s) 271
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 1700
BsiSI CCGG 2 cut(s) 118, 341
BslFI GGGAC 1 cut(s) 828
BslI CCNNNNNNNGG 6 cut(s) 533, 534, 553, 1043, 1294, 1377
BsmAI GTCTC 4 cut(s) 115, 484, 754, 937
BsmFI GGGAC 1 cut(s) 828
BsmI GAATGC 5 cut(s) 67, 103, 371, 1576, 2280
BsnI GGCC 7 cut(s) 445, 982, 1036, 1543, 1564, 2108, 2232
BsoBI CYCGRG 1 cut(s) 1700
Bsp1286I GDGCHC 1 cut(s) 745
Bsp1407I TGTACA 1 cut(s) 2246
Bsp143I GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
Bsp1720I GCTNAGC 1 cut(s) 1937
Bsp68I TCGCGA 1 cut(s) 1812
BspACI CCGC 4 cut(s) 442, 1748, 2112, 2292
BspANI GGCC 7 cut(s) 445, 982, 1036, 1543, 1564, 2108, 2232
BspCNI CTCAG 5 cut(s) 49, 518, 933, 966, 1012
BspFNI CGCG 1 cut(s) 1812
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 3 cut(s) 273, 633, 1369
BspMI ACCTGC 1 cut(s) 1996
BspPI GGATC 5 cut(s) 351, 1362, 1375, 1896, 2134
BspQI GCTCTTC 1 cut(s) 1128
BspT107I GGYRCC 1 cut(s) 271
BsrDI GCAATG 1 cut(s) 332
BsrGI TGTACA 1 cut(s) 2246
BsrI ACTGG 2 cut(s) 275, 1544
BssECI CCNNGG 5 cut(s) 125, 229, 238, 552, 1266
BssMI GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
BssNAI GTATAC 1 cut(s) 810
BssT1I CCWWGG 3 cut(s) 125, 229, 1266
Bst1107I GTATAC 1 cut(s) 810
Bst2UI CCWGG 4 cut(s) 239, 554, 1389, 1561
Bst4CI ACNGT 5 cut(s) 966, 1213, 1380, 1958, 2251
Bst6I CTCTTC 2 cut(s) 816, 1128
BstAUI TGTACA 1 cut(s) 2246
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 5 cut(s) 371, 665, 1761, 2106, 2110
BstF5I GGATG 6 cut(s) 271, 316, 1438, 1896, 2020, 2185
BstFNI CGCG 1 cut(s) 1812
BstKTI GATC 9 cut(s) 346, 1309, 1370, 1768, 1810, 1891, 1931, 2129, 2297
BstMAI GTCTC 4 cut(s) 115, 484, 754, 937
BstMBI GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
BstMWI GCNNNNNNNGC 5 cut(s) 356, 557, 903, 979, 1549
BstNI CCWGG 4 cut(s) 239, 554, 1389, 1561
BstNSI RCATGY 1 cut(s) 667
BstSCI CCNGG 4 cut(s) 237, 552, 1387, 1559
BstSFI CTRYAG 3 cut(s) 28, 1055, 1830
BstUI CGCG 1 cut(s) 1812
BstV1I GCAGC 3 cut(s) 698, 1118, 1202
BstX2I RGATCY 2 cut(s) 1367, 2126
BstYI RGATCY 2 cut(s) 1367, 2126
BstZ17I GTATAC 1 cut(s) 810
BsuRI GGCC 7 cut(s) 445, 982, 1036, 1543, 1564, 2108, 2232
BtgZI GCGATG 2 cut(s) 153, 1827
BtsCI GGATG 6 cut(s) 271, 316, 1438, 1896, 2020, 2185
BtsIMutI CAGTG 4 cut(s) 219, 516, 1209, 1565
BtuMI TCGCGA 1 cut(s) 1812
BveI ACCTGC 1 cut(s) 1996
Cac8I GCNNGC 5 cut(s) 371, 665, 1761, 2106, 2110
CaiI CAGNNNCTG 3 cut(s) 512, 970, 1451
CciI TCATGA 1 cut(s) 624
Cfr13I GGNCC 2 cut(s) 836, 2070
CsiI ACCWGGT 1 cut(s) 1387
Csp6I GTAC 3 cut(s) 2033, 2215, 2247
CviAII CATG 7 cut(s) 21, 625, 664, 793, 1162, 1284, 2054
CviQI GTAC 3 cut(s) 2033, 2215, 2247
DpnI GATC 9 cut(s) 345, 1308, 1369, 1767, 1809, 1890, 1930, 2128, 2296
DpnII GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
DraI TTTAAA 2 cut(s) 1504, 1755
DraIII CACNNNGTG 1 cut(s) 2176
DriI GACNNNNNGTC 1 cut(s) 1391
EaeI YGGCCR 2 cut(s) 980, 2106
Eam1104I CTCTTC 2 cut(s) 816, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 1391
EarI CTCTTC 2 cut(s) 816, 1128
EciI GGCGGA 1 cut(s) 1763
Eco130I CCWWGG 3 cut(s) 125, 229, 1266
Eco47I GGWCC 2 cut(s) 836, 2070
Eco57I CTGAAG 2 cut(s) 837, 840
Eco72I CACGTG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 1700
EcoRII CCWGG 4 cut(s) 237, 552, 1387, 1559
EcoT14I CCWWGG 3 cut(s) 125, 229, 1266
EcoT22I ATGCAT 1 cut(s) 361
ErhI CCWWGG 3 cut(s) 125, 229, 1266
FaeI CATG 7 cut(s) 24, 628, 667, 796, 1165, 1287, 2057
FalI AAGNNNNNCTT 2 cut(s) 1644, 1676
FaqI GGGAC 1 cut(s) 828
FatI CATG 7 cut(s) 20, 624, 663, 792, 1161, 1283, 2053
FauI CCCGC 1 cut(s) 2105
FblI GTMKAC 2 cut(s) 809, 1672
Fnu4HI GCNGC 4 cut(s) 443, 687, 1107, 1191
FokI GGATG 6 cut(s) 278, 323, 1445, 1883, 2027, 2192
Fsp4HI GCNGC 4 cut(s) 443, 687, 1107, 1191
FspBI CTAG 9 cut(s) 87, 141, 230, 543, 749, 813, 936, 1362, 1572
GluI GCNGC 4 cut(s) 443, 687, 1107, 1191
GsaI CCCAGC 1 cut(s) 551
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 7 cut(s) 445, 982, 1036, 1543, 1564, 2108, 2232
HapII CCGG 2 cut(s) 118, 341
Hin1II CATG 7 cut(s) 24, 628, 667, 796, 1165, 1287, 2057
HincII GTYRAC 3 cut(s) 540, 570, 1843
HindII GTYRAC 3 cut(s) 540, 570, 1843
HindIII AAGCTT 2 cut(s) 990, 1508
HpaI GTTAAC 2 cut(s) 540, 1843
HpaII CCGG 2 cut(s) 118, 341
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 6 cut(s) 540, 570, 810, 1673, 1843, 2247
Hpy8I GTNNAC 6 cut(s) 540, 570, 810, 1673, 1843, 2247
Hpy99I CGWCG 1 cut(s) 2123
HpyAV CCTTC 7 cut(s) 27, 41, 413, 456, 622, 1661, 1988
HpyCH4III ACNGT 5 cut(s) 966, 1213, 1380, 1958, 2251
HpyCH4IV ACGT 3 cut(s) 600, 691, 1099
HpyF10VI GCNNNNNNNGC 5 cut(s) 356, 557, 903, 979, 1549
HpySE526I ACGT 3 cut(s) 600, 691, 1099
Hsp92II CATG 7 cut(s) 24, 628, 667, 796, 1165, 1287, 2057
KspAI GTTAAC 2 cut(s) 540, 1843
Kzo9I GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
LguI GCTCTTC 1 cut(s) 1128
LmnI GCTCC 2 cut(s) 637, 2115
Lsp1109I GCAGC 3 cut(s) 698, 1118, 1202
LweI GCATC 3 cut(s) 346, 506, 592
MabI ACCWGGT 1 cut(s) 1387
MaeI CTAG 9 cut(s) 87, 141, 230, 543, 749, 813, 936, 1362, 1572
MaeII ACGT 3 cut(s) 600, 691, 1099
MaeIII GTNAC 6 cut(s) 966, 1200, 1868, 1892, 1985, 2017
MalI GATC 9 cut(s) 345, 1308, 1369, 1767, 1809, 1890, 1930, 2128, 2296
MboI GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
MfeI CAATTG 1 cut(s) 802
MflI RGATCY 2 cut(s) 1367, 2126
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 2108
MluNI TGGCCA 1 cut(s) 2108
MlyI GAGTC 5 cut(s) 117, 500, 1523, 1865, 2271
MmeI TCCRAC 3 cut(s) 312, 1664, 1931
Mox20I TGGCCA 1 cut(s) 2108
Mph1103I ATGCAT 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 1467
MscI TGGCCA 1 cut(s) 2108
MslI CAYNNNNRTG 3 cut(s) 222, 364, 1222
Msp20I TGGCCA 1 cut(s) 2108
MspA1I CMGCKG 3 cut(s) 1835, 2112, 2292
MspI CCGG 2 cut(s) 118, 341
MspR9I CCNGG 4 cut(s) 239, 554, 1389, 1561
MunI CAATTG 1 cut(s) 802
Mva1269I GAATGC 5 cut(s) 67, 103, 371, 1576, 2280
MvaI CCWGG 4 cut(s) 239, 554, 1389, 1561
MvnI CGCG 1 cut(s) 1812
MwoI GCNNNNNNNGC 5 cut(s) 356, 557, 903, 979, 1549
NdeII GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
NlaIII CATG 7 cut(s) 24, 628, 667, 796, 1165, 1287, 2057
NlaIV GGNNCC 3 cut(s) 273, 633, 1369
NmuCI GTSAC 2 cut(s) 1868, 1892
NruI TCGCGA 1 cut(s) 1812
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 1 cut(s) 667
PaeI GCATGC 1 cut(s) 667
PaeR7I CTCGAG 1 cut(s) 1700
PagI TCATGA 1 cut(s) 624
PaqCI CACCTGC 1 cut(s) 1996
PciSI GCTCTTC 1 cut(s) 1128
PctI GAATGC 5 cut(s) 67, 103, 371, 1576, 2280
PdmI GAANNNNTTC 1 cut(s) 1467
PflFI GACNNNGTC 1 cut(s) 931
PkrI GCNGC 4 cut(s) 444, 688, 1108, 1192
PleI GAGTC 5 cut(s) 116, 499, 1522, 1865, 2270
PmaCI CACGTG 1 cut(s) 692
PmlI CACGTG 1 cut(s) 692
PpsI GAGTC 5 cut(s) 116, 499, 1522, 1865, 2270
Ppu21I YACGTR 1 cut(s) 692
Psp6I CCWGG 4 cut(s) 237, 552, 1387, 1559
PspCI CACGTG 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 4 cut(s) 237, 552, 1387, 1559
PspN4I GGNNCC 3 cut(s) 273, 633, 1369
PspPI GGNCC 2 cut(s) 836, 2070
PspXI VCTCGAGB 1 cut(s) 1700
PstNI CAGNNNCTG 3 cut(s) 512, 970, 1451
PsuI RGATCY 2 cut(s) 1367, 2126
PsyI GACNNNGTC 1 cut(s) 931
PvuII CAGCTG 1 cut(s) 1835
RruI TCGCGA 1 cut(s) 1812
RsaI GTAC 3 cut(s) 2034, 2216, 2248
RsaNI GTAC 3 cut(s) 2033, 2215, 2247
RseI CAYNNNNRTG 3 cut(s) 222, 364, 1222
SapI GCTCTTC 1 cut(s) 1128
SatI GCNGC 4 cut(s) 443, 687, 1107, 1191
Sau3AI GATC 9 cut(s) 343, 1306, 1367, 1765, 1807, 1888, 1928, 2126, 2294
Sau96I GGNCC 2 cut(s) 836, 2070
ScaI AGTACT 1 cut(s) 2034
SchI GAGTC 5 cut(s) 117, 500, 1523, 1865, 2271
ScrFI CCNGG 4 cut(s) 239, 554, 1389, 1561
SduI GDGCHC 1 cut(s) 745
SexAI ACCWGGT 1 cut(s) 1387
SfaNI GCATC 3 cut(s) 346, 506, 592
SfcI CTRYAG 3 cut(s) 28, 1055, 1830
Sfr274I CTCGAG 1 cut(s) 1700
SinI GGWCC 2 cut(s) 836, 2070
SlaI CTCGAG 1 cut(s) 1700
SmiMI CAYNNNNRTG 3 cut(s) 222, 364, 1222
SmlI CTYRAG 4 cut(s) 319, 907, 1511, 1700
SmoI CTYRAG 4 cut(s) 319, 907, 1511, 1700
SpeI ACTAGT 1 cut(s) 812
SphI GCATGC 1 cut(s) 667
SsiI CCGC 4 cut(s) 442, 1748, 2112, 2292
SspI AATATT 3 cut(s) 786, 1339, 1860
SspMI CTAG 9 cut(s) 87, 141, 230, 543, 749, 813, 936, 1362, 1572
StyD4I CCNGG 4 cut(s) 237, 552, 1387, 1559
StyI CCWWGG 3 cut(s) 125, 229, 1266
TaaI ACNGT 5 cut(s) 966, 1213, 1380, 1958, 2251
TaiI ACGT 3 cut(s) 603, 694, 1102
TaqI TCGA 5 cut(s) 346, 489, 619, 949, 1701
TatI WGTACW 2 cut(s) 2032, 2246
TauI GCSGC 1 cut(s) 445
TscAI CASTG 4 cut(s) 226, 523, 1216, 1572
TseFI GTSAC 2 cut(s) 1868, 1892
TseI GCWGC 3 cut(s) 686, 1106, 1190
Tsp45I GTSAC 2 cut(s) 1868, 1892
TspGWI ACGGA 1 cut(s) 2215
TspRI CASTG 4 cut(s) 226, 523, 1216, 1572
Tth111I GACNNNGTC 1 cut(s) 931
VpaK11BI GGWCC 2 cut(s) 836, 2070
XapI RAATTY 7 cut(s) 91, 613, 1064, 1154, 1774, 1783, 1820
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 1 cut(s) 667
XcmI CCANNNNNNNNNTGG 1 cut(s) 1403
XhoI CTCGAG 1 cut(s) 1700
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 809, 1672
XmnI GAANNNNTTC 1 cut(s) 1467
XspI CTAG 9 cut(s) 87, 141, 230, 543, 749, 813, 936, 1362, 1572
ZrmI AGTACT 1 cut(s) 2034
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.