Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000964

Physical Location & Seq

Genomic Coordinates Forward (+)

35920540 .. 35926458

Gene Structure 5919 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1247500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2721 bp

ATGTGTGTGTTCTGGCATTCATGCTGCTATAGGCGGATAACAATGGAAGGCTCATCCGAGTCTGCTGGGCAGAATTCTGATAGCTCTAGAGGGTTGAATACTTCCAGAGTCTCAAACCGGAATCCAAGGGTTCATCGGGCTGGACGGTTTGGCTCGTCGGCTGAGGCATTTCAGGATTCGGATTTGAGAAAGGAGAGAGATAGTGTTGTGGTGGCACACACTGATGACCTTACAAACCAGGGTGGATTGTCCGGGGTTTGTGAGGATGAGGTGGCGGTTGACCGTTTTGTCCGAGCTATTGAATGGGGTGATGTTAGCTTGAGGCAGTGGTTGGATAAACCGGATCGATCTGTTGATGTATTTGAATGCTTGCATGTATTTAGGCAAATAGTGGAAATTGTTAATGTAGCACATTCGCAAGGGATTGTTGTTCATAATGTGCGACCTTCATGTTTCGTCATGTCATCTTTCAACCACATCTCCTTTATCGAGTCTGCATCTTGTTCAGATTCTGGCACTGATTCTCCCGAGGATGAGTTAAACAGCCCAACTGAAGAAATTAAGGATTTTCACTCTCTCTTGCATCGTAATTTGCGTCAGCAACGAAGGAACTTAGGAAGAGAAAAATTTCAGTCTATGACAACTCCAAATGCCTTGTCGGAAACGAGTTGCATGCAGTCAAGTTCAATCTATGCAGCACGCGAATCGTTGGTACAAGAGAGTGAAAATGGAATCAGAGACCGAAATGTTGAACTAGAAGATAAGAGACAACCATTTCCAATGAAACATATATTGCTTATGGAGACCAGTTGGTATACTAGTCCAGAAGAGGTTTCTGCTGGATCGAGTTCATGTGCTTCAGACATTTATCGGTTAGGGGTTCTTCTTTTTGAGTTATTCTGTCCACTCAGCTCAAGAGAAGAAAAGAGCAGGACAATGTCTAGTCTGAGACATCGAGTTCTTCCTCCTCAATTGCTGCTGAAATGGCCTAAAGAGGCTTCGTTTTGTTTATGGCTACTGCACCCTGAGCCAAATAGCCGGCCAAAAATGTGTGAGTTGCAACAAAGTGAATTCCTGAATGAACCAAGAGTTGATTTAGAAGAACGTGAAGCGGCAATAGAGCTTAGAGAGAAAATAGAGGAGCAGGAATTATTGCTGGAATTCCTGCTGCTCATACAACAAGGGAAACAGGAATCTGCAGATAAGTTGCAAAATACTCTTTCGTTTCTGTGTTGTGATATTAACGAAGTTATGAAGCACCAAATAACTTCTAAGAAAAAGGGCAGTTCAATCCCACAACTGGTGAAGGAAGATGATTCGACCTCAAGTTTCCCGTCTATGAATATTATTGACGATGATGATTCTGCTAGTGGGTCCAGAAAAAGGTCCAGAACAGGCATCTGGGTTCACAACGTAGAGGAAAGTGATGATAATTTGGATGGTCAGAAGACGGATACTGAAAATCAAGGTGTTCTCTCGAGAAGTTCTAGATTGATGAAGAACTTTAAAAAGCTAGAGGCGGCATATTTTTTAACGAGATGGAGGCCCGTAAAGCAGTCAGCTAAACCGGCTAAACATTCACCAATAAGTAGTTCTGTTGTAACTGAAAGAAGTTCTGTCAATAATTTGCAATCAAAAGAACAACATAGTGGAGGTAGACGAAGTGGTTGGATAGATCCATTTCTTGAGGGATTGTGCAAGTATCTGTCTTTCTCGAAATTAAAAGTGAAGGCAGATTTGAAGCAAGGGGATCTTTTAAATACTTCAAATCTAGTATGCTCACTTAGTTTTGATCGTGATGGAGAATTTTTTGCTACAGCTGGTGTTAATAAGAAAATCAAAGTATTTGAATGTGACACAATAACAAATGAGGATCGTGATATCCACTATCCCGTGGTTGAAATGGCTAGCAGGTCAAAGCTAAGCAGTATATGTTGGAATAGTTATATCAAAAGCCAGATTGCTTCAAGTAACTTTGAAGGTGTGGTGCAGGTATGGGATGTTACAAGAAGTCAATTGTTTACGGAGATGAGAGAACATGAAAGGCGTGTATGGTCCATTGATTTTTCATCAGCAGACCCAACAATGTTGGCTAGCGGGAGTGATGATGGTTCTGTAAAGCTATGGAGTATCAATCAGGCAATTCTACTTTTGCATTTGGGAGTAAGTATTGGTACAATTAAAACAAAGGCGAATGTCTGCTGCGTACAGTTTCCATTGGACTCTAGTCGTTCCCTTGCCTTTGGTTCAGCGGATCATAAAATTTACTACTATGATCTTCGTAACTCAAAGGTTCCTCTGTGCACATTGATCGGACACAACAAAACCGTGAGTTATGTCAAATTTGTGGATGGGACAAATCTCGTTTCTGCGTCCACTGACAATACGGTGAAGCTTTGGGATTTGTCAATGTCCTCATCTCGGGTCATTGACACTCCAGTTCTATCATTCACCGGCCACACGAATGTGAAGAACTTTGTGGGGTTGTCAGTTTCTGATGGTTACATTGCTACAGGCTCAGAGACTAATGAGGTTTTCATCTACCACAAGGCTTTTCCAATGCCGGCATTGTCATACAAGTTTCAGAACACAGACTCACTTTCCGGCCATGACACAGATGATGCTGCACAGTTTATCTCTTCCGTTTGTTGGCGAGGCCAAACCTCCACCTTAATTGCTGCAAATTCGACGGGGAATATAAAAATTTTGGAGATGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0031461 GO:0032991 GO:0042802 GO:0044424 GO:0044464 GO:0050896 GO:0080008 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

Pkinase WD40

Protein Analysis

907

Amino Acids

101.86

Weight (kDa)

5.95

Isoelectric Point (pI)

57.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	587 - 713	6.2e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	589 - 849	2.3e-28	WDR5 beta-propeller domain
WDR55	PF24796	592 - 713	2e-06	WDR55
WD40_CDC20-Fz	PF24807	638 - 738	9.5e-10	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	638 - 847	2.7e-22	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	644 - 821	7.5e-16	TEP-1 second beta-propeller
Beta-prop_EML_2	PF23414	653 - 805	5.7e-11	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	656 - 711	4.9e-07	Prp19 WD40 domain
Beta-prop_WDR3_2nd	PF25172	657 - 802	2.6e-11	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	660 - 812	7e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	675 - 710	7.4e-07	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	677 - 840	3.1e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	721 - 800	2.8e-08	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	728 - 807	5.8e-08	WDHD1 first WD40 domain
WD40_Prp19	PF24814	729 - 841	6.3e-10	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	730 - 842	5.2e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	730 - 848	1.5e-12	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	731 - 904	2.2e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT3G15354 AT3G15354 AT3G15354 AT3G15354 AT3G15354
fragaria_vesca	FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920
malus_domestica	MD03G1228100.v1.1 MD11G1247500.v1.1
prunus_persica	Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1
pyrus_communis	pycom03g17550 pycom11g22000
rosa_chinensis	RchiOBHm_Chr5g0033591
rosa_laevigata	RLG00000033491
rosa_roxburghii	Rroxscaffold_1G00046490
rosa_rugosa	Rorug05G0140300 Rorug05G0140400 Rorug05G0140500 Rorug05G0140600 Rorug05G0140700
rosa_samantha	Rh5AG232000 Rh5BG232700 Rh5CG261600 Rh5DG238300
rosa_wichuraiana	Rw5G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	287
Acc36I	ACCTGC	2 cut(s)	1902, 1981
AccI	GTMKAC	2 cut(s)	815, 1657
AccII	CGCG	1 cut(s)	702
AciI	CCGC	6 cut(s)	34, 275, 1112, 1520, 2097, 2252
AclWI	GGATC	6 cut(s)	351, 850, 1670, 1758, 1881, 2262
AcoI	YGGCCR	3 cut(s)	1040, 2455, 2605
AcsI	RAATTY	9 cut(s)	73, 626, 1070, 1160, 1805, 2262, 2342, 2683, 2703
AcuI	CTGAAG	2 cut(s)	573, 843
AfaI	GTAC	3 cut(s)	714, 2176, 2208
AfiI	CCNNNNNNNGG	4 cut(s)	1300, 1383, 2421, 2649
AhlI	ACTAGT	1 cut(s)	818
AjnI	CCWGG	1 cut(s)	237
AjuI	GAANNNNNNNTTGG	2 cut(s)	1576, 1608
AleI	CACNNNNGTG	1 cut(s)	2465
Alw21I	GWGCWC	1 cut(s)	2306
Alw26I	GTCTC	6 cut(s)	115, 732, 760, 797, 943, 2516
Alw44I	GTGCAC	1 cut(s)	2302
AlwI	GGATC	6 cut(s)	351, 850, 1670, 1758, 1881, 2262
AlwNI	CAGNNNCTG	2 cut(s)	512, 2495
Ama87I	CYCGRG	3 cut(s)	527, 1477, 2421
AoxI	GGCC	6 cut(s)	986, 1040, 1544, 2455, 2605, 2656
ApaLI	GTGCAC	1 cut(s)	2302
ApeKI	GCWGC	7 cut(s)	24, 695, 976, 1168, 2202, 2624, 2678
ApoI	RAATTY	9 cut(s)	73, 626, 1070, 1160, 1805, 2262, 2342, 2683, 2703
ArsI	GACNNNNNNTTYG	2 cut(s)	641, 673
Asp700I	GAANNNNTTC	1 cut(s)	627
AspS9I	GGNCC	4 cut(s)	1374, 1386, 1545, 2055
AsuC2I	CCSGG	1 cut(s)	253
AsuHPI	GGTGA	5 cut(s)	320, 1315, 1572, 2401, 2443
AsuNHI	GCTAGC	2 cut(s)	1907, 2093
AvaI	CYCGRG	3 cut(s)	527, 1477, 2421
AvaII	GGWCC	3 cut(s)	1374, 1386, 2055
BaeGI	GKGCMC	1 cut(s)	2306
BbsI	GAAGAC	1 cut(s)	1454
Bbv12I	GWGCWC	1 cut(s)	2306
BbvCI	CCTCAGC	1 cut(s)	162
BbvI	GCAGC	7 cut(s)	11, 707, 963, 1155, 2189, 2611, 2665
BccI	CCATC	7 cut(s)	1433, 1533, 1793, 2102, 2345, 2492, 2707
BcgI	CGANNNNNNTGC	6 cut(s)	655, 687, 689, 721, 2293, 2327
BciT130I	CCWGG	1 cut(s)	239
BciVI	GTATCC	1 cut(s)	1447
BcnI	CCSGG	1 cut(s)	253
BcoDI	GTCTC	6 cut(s)	115, 732, 760, 797, 943, 2516
BcuI	ACTAGT	1 cut(s)	818
BfmI	CTRYAG	4 cut(s)	28, 1197, 1815, 2511
BfuAI	ACCTGC	2 cut(s)	1902, 1981
BfuI	GTATCC	1 cut(s)	1447
BisI	GCNGC	9 cut(s)	25, 696, 977, 1113, 1169, 1521, 2203, 2625, 2679
BlpI	GCTNAGC	1 cut(s)	1922
BlsI	GCNGC	9 cut(s)	26, 697, 978, 1114, 1170, 1522, 2204, 2626, 2680
Bme1390I	CCNGG	2 cut(s)	239, 253
Bme18I	GGWCC	3 cut(s)	1374, 1386, 2055
BmeT110I	CYCGRG	3 cut(s)	527, 1477, 2421
BmgT120I	GGNCC	4 cut(s)	1374, 1386, 1545, 2055
BmiI	GGNNCC	2 cut(s)	1375, 2295
BmrFI	CCNGG	2 cut(s)	239, 253
BmsI	GCATC	4 cut(s)	506, 592, 1407, 2611
BmtI	GCTAGC	2 cut(s)	1911, 2097
BoxI	GACNNNNGTC	1 cut(s)	2226
BpiI	GAAGAC	1 cut(s)	1454
BpmI	CTGGAG	1 cut(s)	2421
Bpu10I	CCTNAGC	2 cut(s)	162, 1026
Bpu1102I	GCTNAGC	1 cut(s)	1922
BpuEI	CTTGAG	4 cut(s)	340, 898, 1309, 1706
BpuMI	CCSGG	1 cut(s)	253
Bsa29I	ATCGAT	1 cut(s)	346
BsaI	GGTCTC	2 cut(s)	732, 797
BsaJI	CCNNGG	5 cut(s)	125, 238, 252, 528, 1893
BsaWI	WCCGGW	2 cut(s)	117, 340
BsaXI	ACNNNNNCTCC	4 cut(s)	464, 494, 508, 538
Bsc4I	CCNNNNNNNGG	4 cut(s)	1300, 1383, 2421, 2649
Bse118I	RCCGGY	4 cut(s)	1038, 1567, 2453, 2563
Bse1I	ACTGG	3 cut(s)	807, 1305, 2438
Bse3DI	GCAATG	1 cut(s)	2505
BseBI	CCWGG	1 cut(s)	239
BseCI	ATCGAT	1 cut(s)	346
BseDI	CCNNGG	5 cut(s)	125, 238, 252, 528, 1893
BseGI	GGATG	6 cut(s)	53, 271, 538, 1444, 2005, 2356
BseLI	CCNNNNNNNGG	4 cut(s)	1300, 1383, 2421, 2649
BseMI	GCAATG	1 cut(s)	2505
BseMII	CTCAG	5 cut(s)	153, 922, 938, 1017, 2532
BseNI	ACTGG	3 cut(s)	807, 1305, 2438
BseRI	GAGGAG	2 cut(s)	957, 1154
BseSI	GKGCMC	1 cut(s)	2306
BseXI	GCAGC	7 cut(s)	11, 707, 963, 1155, 2189, 2611, 2665
BseYI	CCCAGC	1 cut(s)	65
BsgI	GTGCAG	3 cut(s)	1004, 2009, 2610
Bsh1236I	CGCG	1 cut(s)	702
BshFI	GGCC	6 cut(s)	988, 1042, 1546, 2457, 2607, 2658
BshVI	ATCGAT	1 cut(s)	346
BsiHKAI	GWGCWC	1 cut(s)	2306
BsiHKCI	CYCGRG	3 cut(s)	527, 1477, 2421
BsiSI	CCGG	8 cut(s)	118, 252, 341, 1039, 1568, 2454, 2564, 2604
BslFI	GGGAC	1 cut(s)	2368
BslI	CCNNNNNNNGG	4 cut(s)	1300, 1383, 2421, 2649
BsmAI	GTCTC	6 cut(s)	115, 732, 760, 797, 943, 2516
BsmFI	GGGAC	1 cut(s)	2368
BsmI	GAATGC	2 cut(s)	16, 371
BsnI	GGCC	6 cut(s)	988, 1042, 1546, 2457, 2607, 2658
Bso31I	GGTCTC	2 cut(s)	732, 797
BsoBI	CYCGRG	3 cut(s)	527, 1477, 2421
Bsp1286I	GDGCHC	1 cut(s)	2306
Bsp1720I	GCTNAGC	1 cut(s)	1922
BspACI	CCGC	6 cut(s)	34, 275, 1112, 1520, 2097, 2252
BspANI	GGCC	6 cut(s)	988, 1042, 1546, 2457, 2607, 2658
BspCNI	CTCAG	5 cut(s)	154, 921, 939, 1018, 2531
BspDI	ATCGAT	1 cut(s)	346
BspFNI	CGCG	1 cut(s)	702
BspLI	GGNNCC	2 cut(s)	1375, 2295
BspMAI	CTGCAG	1 cut(s)	1201
BspMI	ACCTGC	2 cut(s)	1902, 1981
BspOI	GCTAGC	2 cut(s)	1911, 2097
BspPI	GGATC	6 cut(s)	351, 850, 1670, 1758, 1881, 2262
BspTNI	GGTCTC	2 cut(s)	732, 797
BsrDI	GCAATG	1 cut(s)	2505
BsrFI	RCCGGY	4 cut(s)	1038, 1567, 2453, 2563
BsrI	ACTGG	3 cut(s)	807, 1305, 2438
BssAI	RCCGGY	4 cut(s)	1038, 1567, 2453, 2563
BssECI	CCNNGG	5 cut(s)	125, 238, 252, 528, 1893
BssNAI	GTATAC	1 cut(s)	816
BssT1I	CCWWGG	1 cut(s)	125
Bst1107I	GTATAC	1 cut(s)	816
Bst2UI	CCWGG	1 cut(s)	239
Bst4CI	ACNGT	6 cut(s)	147, 284, 2211, 2329, 2389, 2631
Bst6I	CTCTTC	3 cut(s)	613, 822, 2644
BstC8I	GCNNGC	7 cut(s)	371, 674, 700, 1040, 1909, 2095, 2565
BstDSI	CCRYGG	1 cut(s)	1893
BstF5I	GGATG	6 cut(s)	53, 271, 538, 1444, 2005, 2356
BstFNI	CGCG	1 cut(s)	702
BstMAI	GTCTC	6 cut(s)	115, 732, 760, 797, 943, 2516
BstMWI	GCNNNNNNNGC	4 cut(s)	985, 1027, 1552, 1568
BstNI	CCWGG	1 cut(s)	239
BstNSI	RCATGY	2 cut(s)	377, 676
BstPAI	GACNNNNGTC	1 cut(s)	2226
BstSCI	CCNGG	2 cut(s)	237, 251
BstSFI	CTRYAG	4 cut(s)	28, 1197, 1815, 2511
BstSLI	GKGCMC	1 cut(s)	2306
BstUI	CGCG	1 cut(s)	702
BstV1I	GCAGC	7 cut(s)	11, 707, 963, 1155, 2189, 2611, 2665
BstV2I	GAAGAC	1 cut(s)	1454
BstX2I	RGATCY	2 cut(s)	1675, 1750
BstYI	RGATCY	2 cut(s)	1675, 1750
BstZ17I	GTATAC	1 cut(s)	816
Bsu15I	ATCGAT	1 cut(s)	346
BsuI	GTATCC	1 cut(s)	1447
BsuRI	GGCC	6 cut(s)	988, 1042, 1546, 2457, 2607, 2658
BsuTUI	ATCGAT	1 cut(s)	346
BtgI	CCRYGG	1 cut(s)	1893
BtsCI	GGATG	6 cut(s)	53, 271, 538, 1444, 2005, 2356
BtsI	GCAGTG	1 cut(s)	332
BtsIMutI	CAGTG	4 cut(s)	219, 332, 516, 2376
BveI	ACCTGC	2 cut(s)	1902, 1981
Cac8I	GCNNGC	7 cut(s)	371, 674, 700, 1040, 1909, 2095, 2565
CaiI	CAGNNNCTG	2 cut(s)	512, 2495
Cfr10I	RCCGGY	4 cut(s)	1038, 1567, 2453, 2563
Cfr13I	GGNCC	4 cut(s)	1374, 1386, 1545, 2055
ClaI	ATCGAT	1 cut(s)	346
CseI	GACGC	2 cut(s)	584, 2361
Csp6I	GTAC	3 cut(s)	713, 2175, 2207
CspCI	CAANNNNNGTGG	2 cut(s)	2656, 2691
CviAII	CATG	8 cut(s)	21, 374, 450, 460, 673, 852, 2039, 2609
CviQI	GTAC	3 cut(s)	713, 2175, 2207
DraI	TTTAAA	2 cut(s)	1507, 1758
DrdI	GACNNNNNNGTC	1 cut(s)	287
DseDI	GACNNNNNNGTC	1 cut(s)	287
EaeI	YGGCCR	3 cut(s)	1040, 2455, 2605
Eam1104I	CTCTTC	3 cut(s)	613, 822, 2644
EarI	CTCTTC	3 cut(s)	613, 822, 2644
EciI	GGCGGA	1 cut(s)	49
Eco130I	CCWWGG	1 cut(s)	125
Eco31I	GGTCTC	2 cut(s)	732, 797
Eco32I	GATATC	1 cut(s)	1882
Eco47I	GGWCC	3 cut(s)	1374, 1386, 2055
Eco57I	CTGAAG	2 cut(s)	573, 843
Eco88I	CYCGRG	3 cut(s)	527, 1477, 2421
EcoRI	GAATTC	3 cut(s)	73, 1070, 1160
EcoRII	CCWGG	1 cut(s)	237
EcoRV	GATATC	1 cut(s)	1882
EcoT14I	CCWWGG	1 cut(s)	125
ErhI	CCWWGG	1 cut(s)	125
FaeI	CATG	8 cut(s)	24, 377, 453, 463, 676, 855, 2042, 2612
FalI	AAGNNNNNCTT	2 cut(s)	1737, 1769
FaqI	GGGAC	1 cut(s)	2368
FatI	CATG	8 cut(s)	20, 373, 449, 459, 672, 851, 2038, 2608
FauI	CCCGC	1 cut(s)	2090
FblI	GTMKAC	2 cut(s)	815, 1657
Fnu4HI	GCNGC	9 cut(s)	25, 696, 977, 1113, 1169, 1521, 2203, 2625, 2679
FokI	GGATG	6 cut(s)	40, 278, 545, 1451, 2012, 2363
Fsp4HI	GCNGC	9 cut(s)	25, 696, 977, 1113, 1169, 1521, 2203, 2625, 2679
GluI	GCNGC	9 cut(s)	25, 696, 977, 1113, 1169, 1521, 2203, 2625, 2679
GsaI	CCCAGC	1 cut(s)	69
GsuI	CTGGAG	1 cut(s)	2421
HaeIII	GGCC	6 cut(s)	988, 1042, 1546, 2457, 2607, 2658
HapII	CCGG	8 cut(s)	118, 252, 341, 1039, 1568, 2454, 2564, 2604
HgaI	GACGC	2 cut(s)	584, 2361
Hin1II	CATG	8 cut(s)	24, 377, 453, 463, 676, 855, 2042, 2612
HincII	GTYRAC	1 cut(s)	280
HindII	GTYRAC	1 cut(s)	280
HindIII	AAGCTT	1 cut(s)	2393
HpaII	CCGG	8 cut(s)	118, 252, 341, 1039, 1568, 2454, 2564, 2604
HphI	GGTGA	5 cut(s)	320, 1315, 1572, 2401, 2443
Hpy166II	GTNNAC	8 cut(s)	280, 816, 905, 1408, 1658, 2022, 2304, 2376
Hpy8I	GTNNAC	8 cut(s)	280, 816, 905, 1408, 1658, 2022, 2304, 2376
Hpy99I	CGWCG	2 cut(s)	160, 2692
HpyAV	CCTTC	6 cut(s)	41, 456, 600, 1300, 1723, 1973
HpyCH4III	ACNGT	6 cut(s)	147, 284, 2211, 2329, 2389, 2631
HpyCH4IV	ACGT	2 cut(s)	1105, 1413
HpyF10VI	GCNNNNNNNGC	4 cut(s)	985, 1027, 1552, 1568
HpySE526I	ACGT	2 cut(s)	1105, 1413
Hsp92II	CATG	8 cut(s)	24, 377, 453, 463, 676, 855, 2042, 2612
KroI	GCCGGC	2 cut(s)	1038, 2563
KroNI	GCCGGC	2 cut(s)	1040, 2565
LmnI	GCTCC	1 cut(s)	1141
Lsp1109I	GCAGC	7 cut(s)	11, 707, 963, 1155, 2189, 2611, 2665
LweI	GCATC	4 cut(s)	506, 592, 1407, 2611
MaeII	ACGT	2 cut(s)	1105, 1413
MaeIII	GTNAC	6 cut(s)	1600, 1853, 1970, 2002, 2282, 2501
MfeI	CAATTG	2 cut(s)	971, 2015
MflI	RGATCY	2 cut(s)	1675, 1750
MhlI	GDGCHC	1 cut(s)	2306
MlyI	GAGTC	5 cut(s)	68, 117, 500, 2216, 2588
MmeI	TCCRAC	4 cut(s)	312, 639, 1649, 1916
MroNI	GCCGGC	2 cut(s)	1038, 2563
MroXI	GAANNNNTTC	1 cut(s)	627
MslI	CAYNNNNRTG	3 cut(s)	222, 2463, 2465
MspA1I	CMGCKG	2 cut(s)	1820, 2252
MspI	CCGG	8 cut(s)	118, 252, 341, 1039, 1568, 2454, 2564, 2604
MspR9I	CCNGG	2 cut(s)	239, 253
MunI	CAATTG	2 cut(s)	971, 2015
Mva1269I	GAATGC	2 cut(s)	16, 371
MvaI	CCWGG	1 cut(s)	239
MvnI	CGCG	1 cut(s)	702
MwoI	GCNNNNNNNGC	4 cut(s)	985, 1027, 1552, 1568
NaeI	GCCGGC	2 cut(s)	1040, 2565
NciI	CCSGG	1 cut(s)	253
NgoMIV	GCCGGC	2 cut(s)	1038, 2563
NheI	GCTAGC	2 cut(s)	1907, 2093
NlaIII	CATG	8 cut(s)	24, 377, 453, 463, 676, 855, 2042, 2612
NlaIV	GGNNCC	2 cut(s)	1375, 2295
NmuCI	GTSAC	1 cut(s)	1853
NspI	RCATGY	2 cut(s)	377, 676
OliI	CACNNNNGTG	1 cut(s)	2465
PaeI	GCATGC	1 cut(s)	676
PaeR7I	CTCGAG	1 cut(s)	1477
PcsI	WCGNNNNNNNCGW	1 cut(s)	592
PctI	GAATGC	2 cut(s)	16, 371
PdiI	GCCGGC	2 cut(s)	1040, 2565
PdmI	GAANNNNTTC	1 cut(s)	627
PfeI	GAWTC	9 cut(s)	121, 176, 509, 521, 704, 732, 1193, 1316, 1361
PflFI	GACNNNGTC	1 cut(s)	937
PkrI	GCNGC	9 cut(s)	26, 697, 978, 1114, 1170, 1522, 2204, 2626, 2680
PleI	GAGTC	5 cut(s)	67, 116, 499, 2216, 2588
PpsI	GAGTC	5 cut(s)	67, 116, 499, 2216, 2588
PshAI	GACNNNNGTC	1 cut(s)	2226
Psp6I	CCWGG	1 cut(s)	237
PspFI	CCCAGC	1 cut(s)	65
PspGI	CCWGG	1 cut(s)	237
PspN4I	GGNNCC	2 cut(s)	1375, 2295
PspPI	GGNCC	4 cut(s)	1374, 1386, 1545, 2055
PstI	CTGCAG	1 cut(s)	1201
PstNI	CAGNNNCTG	2 cut(s)	512, 2495
PsuI	RGATCY	2 cut(s)	1675, 1750
PsyI	GACNNNGTC	1 cut(s)	937
PvuII	CAGCTG	1 cut(s)	1820
RsaI	GTAC	3 cut(s)	714, 2176, 2208
RsaNI	GTAC	3 cut(s)	713, 2175, 2207
RseI	CAYNNNNRTG	3 cut(s)	222, 2463, 2465
SatI	GCNGC	9 cut(s)	25, 696, 977, 1113, 1169, 1521, 2203, 2625, 2679
Sau96I	GGNCC	4 cut(s)	1374, 1386, 1545, 2055
SchI	GAGTC	5 cut(s)	68, 117, 500, 2216, 2588
ScrFI	CCNGG	2 cut(s)	239, 253
SduI	GDGCHC	1 cut(s)	2306
SfaNI	GCATC	4 cut(s)	506, 592, 1407, 2611
SfcI	CTRYAG	4 cut(s)	28, 1197, 1815, 2511
Sfr274I	CTCGAG	1 cut(s)	1477
SinI	GGWCC	3 cut(s)	1374, 1386, 2055
SlaI	CTCGAG	1 cut(s)	1477
SmiMI	CAYNNNNRTG	3 cut(s)	222, 2463, 2465
SmlI	CTYRAG	5 cut(s)	319, 913, 1324, 1477, 1685
SmoI	CTYRAG	5 cut(s)	319, 913, 1324, 1477, 1685
SpeI	ACTAGT	1 cut(s)	818
SphI	GCATGC	1 cut(s)	676
SsiI	CCGC	6 cut(s)	34, 275, 1112, 1520, 2097, 2252
SspI	AATATT	1 cut(s)	1345
StyD4I	CCNGG	2 cut(s)	237, 251
StyI	CCWWGG	1 cut(s)	125
TaaI	ACNGT	6 cut(s)	147, 284, 2211, 2329, 2389, 2631
TaiI	ACGT	2 cut(s)	1108, 1416
TaqI	TCGA	8 cut(s)	346, 489, 845, 955, 1319, 1478, 1715, 2687
TaqII	GACCGA	1 cut(s)	756
TauI	GCSGC	2 cut(s)	1115, 1523
TfiI	GAWTC	9 cut(s)	121, 176, 509, 521, 704, 732, 1193, 1316, 1361
TscAI	CASTG	4 cut(s)	226, 332, 523, 2383
TseFI	GTSAC	1 cut(s)	1853
TseI	GCWGC	7 cut(s)	24, 695, 976, 1168, 2202, 2624, 2678
Tsp45I	GTSAC	1 cut(s)	1853
TspGWI	ACGGA	3 cut(s)	1466, 2039, 2632
TspRI	CASTG	4 cut(s)	226, 332, 523, 2383
Tth111I	GACNNNGTC	1 cut(s)	937
VneI	GTGCAC	1 cut(s)	2302
VpaK11BI	GGWCC	3 cut(s)	1374, 1386, 2055
XapI	RAATTY	9 cut(s)	73, 626, 1070, 1160, 1805, 2262, 2342, 2683, 2703
XbaI	TCTAGA	2 cut(s)	86, 1487
XceI	RCATGY	2 cut(s)	377, 676
XhoI	CTCGAG	1 cut(s)	1477
XmiI	GTMKAC	2 cut(s)	815, 1657
XmnI	GAANNNNTTC	1 cut(s)	627

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.