Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0000964

Physical Location & Seq

Genomic Coordinates Reverse (-)

27967422 .. 27973555

Gene Structure 6134 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGTTGTACCCTTTTCATGGTGGAACTGGAAATCTGTCTGTCGTTGACAAGTTGTTGATGTGCTCTGCTTGGAGGATAATAATGGAAGGCTCATCCGAGTCTGCTTGGCAGAGGTCTAATAGTTCTAGAGATTTGAATACTTCCAGAGTCTCAAACCGGAATCCAAGGCTTAGTCATTCTAGAAGATTCGGCTTCTTGGGTGGTTCATCCCAGGATTCGGATTTGAGGAATGAGAGAGATGGGGTTGTGGTGGCACACACTGATGACCTAGGAAACCAGGGTGGATTGTCTGGGGTGTGTGAGGATGAGGTACCAGTTGAACCTTTTGTTCGGGCCATTGAATGGGGAGATGTTAGCTTGAGGCAATGGTTGGATAGACCGGATCGATCTGTTGATGCATTTGAATGCTTGCATATATTTAGGCAAATAGTGGAAATTGTTAATGTAGCACATTCAGAAGGAATTGTTGTTCATAATGTGCGGCCTTCATGCTTTGTTATGTCCTCTTTTAATCATGTCTCTTTCATCGAGTCTGCATCTTGCTCAGATTCTGGCACTGATTCTCCCGAGGATGGGTTAAATAGCCCAACCTTGGAAGCGAAGAACTTAACCTCTGCCTTGCATCAGAAACGAAGCAACATAGCAAGAGGAAATTTTCAATTTGTGAAAGCTCCAGCAAATACTTTGTCAGATACAAGTTTCATGCAGTCAAGCTCAATATATGCAGCGCGTGAATCATTAATGCAAGAGAGTGAAGAACATAGAACCAGAGAGAGGAGTGCTCAACTAGAAGATAAGAGACAGCCATTTCCAATGAAACAGATATTGCTCATGGAAAGCAATTGGTATACTAGTCCCGAAGAGGTTGCTGGTGGTCCAAGTCCCTGTGCGTCAGACATTTACCGATTAGGAGTTCTTCTTTTTGAGTTATTCTGCCCATTCAGCTCAAGAGAAGAAAAGAGTAGGACTATGTCTAGTCTGAGACATCGAGTTCTACCTCCGCAATTACTGCTGAGATGGCCAAAAGAAGCTGCATTTTGCTTATGGCTACTGCACCCTGAGCCAAATAGTCGGCCTAAAATGGGTGAATTGCTACAGAGTGAATTTCTTAATGAACCAAGAGATGATTTAGAAGAACGTGAAGCAGCAATAGAGCTTAGAGAGAAAATAGAGGAGCAGGAGTTATTGCTGGAATTTCTCTTGCTTGTAAAACAAAGGAAACAGGAAGCAGCCGATAAGTTGCGGAATACTGTTTCATTTCTGTGTTCCGATATTGAAGAAGTTGTGAAGCACAAAACAAGTTCCAAGGGAAAAGGTGGTTCATGCCCAGAGCTGGTAAAGGAAGATCATTCAACATCAAGTTTCCCATCTATGAATATTACTGATGATGATGATTCGGCTAGTGGATCCAGAAAACGGTTCAGACCAGGCGTCCATATTCACAATGGAGAGGAGTGTGATGATAATTTGGATGGTCAGAAGTCAGAAACTGATAATCAAGAAAGTATTCTTTTGAGAAGTTCCAGATTGATGAACAACTTTAAAAAGCTAGAGTCGGCATATTTTTTGACGAGATGGAGGCCAATCAAGCACTCAGCCAAACCCTTGACTAGACATTCATCAATAAGTAGTGATGGTAGAGGTTCTATTGTTGCAACTGAAAGAAGTTCTGTCAGTAATTTGTCATTAAAAGAACAACATAGTGAAGGTAGACGAAGTGGTTGGATAGAACCATTTCTTGAGGGATTATGCAAGTATCTATCTTTCTCAAAATTAAAAGTGAAGGCGGATTTGAAGCAGGCAGATCTTCTGAATTCCTCAAATCTAGTATGCTCACTTAGTTTTGATCGGGATGGAGAATTTTTTGCTACGGCTGGTGTTAATAAGAAAATCAAAATATTTGAATGTGACTCAATCATAAACGAGGATCGTGATATCCATTATCCTGTAGTTGAAATAGCTACCAGATCAAAGCTAAGCAATATATGTTGGAACAGTTATATCAAAAGCCAGATTGCTTCAAGTAACTTTGAAGGTGTAGTGCAGGTGTGGGATGTTACAAGAAGTCAAGTACTAATGGAGATGAAAGAACATGAGAAGCGTGTATGGTCCATTGACTTTTCATCAGCAGACCCAACAATGCTAGCCAGCGGGAGTGATGATGGTTCTGTTAAGCTATGGAGTATCAATCAGGGAGAAAGTATTGGTACAATAAAAACAAAGGCCAATGTCTGCTGTGTACAGTTTCCTTTGGATTCTTGTCGTTCCCTTGCATTCGGTTCAGCGGATCATAAAATTTATTACTATGATCTTCGTAACTCGAAGGTTCCTCTTTGTACATTGATTGGACACAACAAAACTGTGAGTTATGTCAAGTTCATAGATAGGATGAATCTTGTTTCTGCATCCACCGACAACACACTGAAGCTTTGGGATTTGTCAACATGCACATCCCGAGTCATAGACACTCCGGTTATGACATTCACAGGTCACACGAATGTGAAGAACTTTGTGGGTTTGTCAGTTTCTGATGGTTACATTGCTACCGGCTCAGAGACTAATGAGGTTTTCGTCTACCACAAGGCGTTCCCAATGCCGGCGTTGTCGTACAAGTTTCAGGCCACAGACCCGCTTTCTGGCCAGGATACAGATGATGCTGCACAATTTATCTCATCTGTTTGTTGGCGTGGCCAATCCTCCACCTTAATTGCTGCAAATTCCACAGGGAATATCAAAATCCTGGAGATGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0031461 GO:0032991 GO:0042802 GO:0044424 GO:0044464 GO:0050896 GO:0080008 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

Pkinase WD40

Protein Analysis

918

Amino Acids

102.82

Weight (kDa)

5.9

Isoelectric Point (pI)

59.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	600 - 743	1.7e-13	WDR3 first beta-propeller domain
Beta-prop_WDR19_1st	PF23389	603 - 749	6e-06	WDR19 first beta-propeller
Beta-prop_WDR5	PF25175	607 - 860	7.4e-32	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	607 - 775	5.4e-06	G protein beta WD-40 repeat protein
WDR55	PF24796	607 - 734	6.4e-07	WDR55
WD40_CDC20-Fz	PF24807	656 - 749	1e-13	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	657 - 856	1.3e-24	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	670 - 818	8.4e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	671 - 740	1.6e-06	WDHD1 first WD40 domain
WD40_Prp19	PF24814	674 - 749	3.5e-10	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	675 - 811	3e-16	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	677 - 859	1.8e-26	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	677 - 815	7.1e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	691 - 728	1.7e-07	WD domain, G-beta repeat
EIF3I	PF24805	698 - 753	9e-07	EIF3I
Beta-prop_WDR3_2nd	PF25172	709 - 829	4.1e-10	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	709 - 861	1.1e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	714 - 850	1.2e-08	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	739 - 817	3.9e-07	WDHD1 first WD40 domain
WD40_Prp19	PF24814	740 - 852	1.6e-08	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	741 - 833	1.2e-07	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	755 - 852	7.9e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	755 - 915	7.4e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT3G15354 AT3G15354 AT3G15354 AT3G15354 AT3G15354
fragaria_vesca	FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920
malus_domestica	MD03G1228100.v1.1 MD11G1247500.v1.1
prunus_persica	Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1
pyrus_communis	pycom03g17550 pycom11g22000
rosa_chinensis	RchiOBHm_Chr5g0033591
rosa_laevigata	RLG00000033491
rosa_roxburghii	Rroxscaffold_1G00046490
rosa_rugosa	Rorug05G0140300 Rorug05G0140400 Rorug05G0140500 Rorug05G0140600 Rorug05G0140700
rosa_samantha	Rh5AG232000 Rh5BG232700 Rh5CG261600 Rh5DG238300
rosa_wichuraiana	Rw5G021170

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2035
Acc36I	ACCTGC	1 cut(s)	2035
Acc65I	GGTACC	1 cut(s)	310
AccB1I	GGYRCC	1 cut(s)	310
AccB7I	CCANNNNNTGG	1 cut(s)	342
AccI	GTMKAC	3 cut(s)	848, 1711, 2574
AccII	CGCG	1 cut(s)	730
AciI	CCGC	7 cut(s)	481, 1001, 1243, 1787, 2151, 2285, 2630
AclWI	GGATC	5 cut(s)	390, 1401, 1414, 1935, 2295
AcoI	YGGCCR	3 cut(s)	1019, 2638, 2689
AcsI	RAATTY	7 cut(s)	652, 1103, 1193, 1813, 1859, 2295, 2716
AcuI	CTGAAG	1 cut(s)	2444
AcyI	GRCGYC	1 cut(s)	1431
AfaI	GTAC	7 cut(s)	8, 312, 2073, 2209, 2241, 2338, 2609
AhdI	GACNNNNNGTC	1 cut(s)	1430
AhlI	ACTAGT	1 cut(s)	851
AjnI	CCWGG	5 cut(s)	210, 276, 1426, 2640, 2739
AleI	CACNNNNGTG	1 cut(s)	2498
AloI	GAACNNNNNNTCC	2 cut(s)	453, 485
Alw21I	GWGCWC	2 cut(s)	65, 784
Alw26I	GTCTC	5 cut(s)	154, 523, 793, 976, 2549
AlwI	GGATC	5 cut(s)	390, 1401, 1414, 1935, 2295
AlwNI	CAGNNNCTG	3 cut(s)	551, 1490, 2528
Ama87I	CYCGRG	2 cut(s)	566, 2454
AoxI	GGCC	9 cut(s)	333, 482, 1019, 1073, 1580, 2223, 2619, 2638, 2689
ApeKI	GCWGC	6 cut(s)	725, 1031, 1145, 1229, 2657, 2711
ApoI	RAATTY	7 cut(s)	652, 1103, 1193, 1813, 1859, 2295, 2716
ArsI	GACNNNNNNTTYG	2 cut(s)	671, 703
AseI	ATTAAT	1 cut(s)	740
Asp700I	GAANNNNTTC	2 cut(s)	1506, 1734
Asp718I	GGTACC	1 cut(s)	310
AspA2I	CCTAGG	1 cut(s)	268
AspLEI	GCGC	1 cut(s)	730
AspS9I	GGNCC	3 cut(s)	333, 875, 2109
AsuHPI	GGTGA	1 cut(s)	1097
AsuNHI	GCTAGC	1 cut(s)	2143
AvaI	CYCGRG	2 cut(s)	566, 2454
AvaII	GGWCC	2 cut(s)	875, 2109
AvrII	CCTAGG	1 cut(s)	268
BalI	TGGCCA	3 cut(s)	1021, 2640, 2691
BamHI	GGATCC	1 cut(s)	1406
BanI	GGYRCC	1 cut(s)	310
Bbv12I	GWGCWC	2 cut(s)	65, 784
BbvI	GCAGC	6 cut(s)	737, 1018, 1157, 1241, 2644, 2698
BceAI	ACGGC	1 cut(s)	1887
BcgI	CGANNNNNNTGC	2 cut(s)	848, 882
BciT130I	CCWGG	5 cut(s)	212, 278, 1428, 2642, 2741
BciVI	GTATCC	1 cut(s)	2638
BcoDI	GTCTC	5 cut(s)	154, 523, 793, 976, 2549
BcuI	ACTAGT	1 cut(s)	851
BfmI	CTRYAG	2 cut(s)	1094, 1947
BfuAI	ACCTGC	1 cut(s)	2035
BfuI	GTATCC	1 cut(s)	2638
BglII	AGATCT	1 cut(s)	1804
BisI	GCNGC	7 cut(s)	482, 726, 1032, 1146, 1230, 2658, 2712
BlnI	CCTAGG	1 cut(s)	268
BlpI	GCTNAGC	1 cut(s)	1976
BlsI	GCNGC	7 cut(s)	483, 727, 1033, 1147, 1231, 2659, 2713
BmcAI	AGTACT	1 cut(s)	2073
Bme1390I	CCNGG	5 cut(s)	212, 278, 1428, 2642, 2741
Bme18I	GGWCC	2 cut(s)	875, 2109
BmeRI	GACNNNNNGTC	1 cut(s)	1430
BmeT110I	CYCGRG	2 cut(s)	566, 2454
BmgT120I	GGNCC	3 cut(s)	333, 875, 2109
BmiI	GGNNCC	3 cut(s)	312, 1408, 2328
BmrFI	CCNGG	5 cut(s)	212, 278, 1428, 2642, 2741
BmsI	GCATC	5 cut(s)	385, 545, 631, 2414, 2644
BmtI	GCTAGC	1 cut(s)	2147
BplI	GAGNNNNNCTC	2 cut(s)	766, 798
BpmI	CTGGAG	1 cut(s)	657
Bpu10I	CCTNAGC	1 cut(s)	1059
Bpu1102I	GCTNAGC	1 cut(s)	1976
BpuEI	CTTGAG	3 cut(s)	379, 931, 1760
Bsa29I	ATCGAT	1 cut(s)	385
BsaHI	GRCGYC	1 cut(s)	1431
BsaJI	CCNNGG	7 cut(s)	164, 210, 268, 277, 567, 591, 1305
BsaWI	WCCGGW	3 cut(s)	156, 379, 2470
BsaXI	ACNNNNNCTCC	4 cut(s)	547, 577, 1166, 1196
Bse118I	RCCGGY	2 cut(s)	2546, 2596
Bse1I	ACTGG	2 cut(s)	31, 314
Bse3DI	GCAATG	2 cut(s)	371, 2538
BseBI	CCWGG	5 cut(s)	212, 278, 1428, 2642, 2741
BseCI	ATCGAT	1 cut(s)	385
BseDI	CCNNGG	7 cut(s)	164, 210, 268, 277, 567, 591, 1305
BseMI	GCAATG	2 cut(s)	371, 2538
BseMII	CTCAG	6 cut(s)	558, 971, 1004, 1050, 1608, 2565
BseNI	ACTGG	2 cut(s)	31, 314
BseRI	GAGGAG	3 cut(s)	790, 1187, 1466
BseXI	GCAGC	6 cut(s)	737, 1018, 1157, 1241, 2644, 2698
BsgI	GTGCAG	3 cut(s)	1037, 2063, 2643
Bsh1236I	CGCG	1 cut(s)	730
BshFI	GGCC	9 cut(s)	335, 484, 1021, 1075, 1582, 2225, 2621, 2640, 2691
BshNI	GGYRCC	1 cut(s)	310
BshVI	ATCGAT	1 cut(s)	385
BsiHKAI	GWGCWC	2 cut(s)	65, 784
BsiHKCI	CYCGRG	2 cut(s)	566, 2454
BsiSI	CCGG	5 cut(s)	157, 380, 2471, 2547, 2597
BslFI	GGGAC	2 cut(s)	840, 867
BsmAI	GTCTC	5 cut(s)	154, 523, 793, 976, 2549
BsmFI	GGGAC	2 cut(s)	840, 867
BsmI	GAATGC	2 cut(s)	410, 2273
BsnI	GGCC	9 cut(s)	335, 484, 1021, 1075, 1582, 2225, 2621, 2640, 2691
BsoBI	CYCGRG	2 cut(s)	566, 2454
Bsp1286I	GDGCHC	2 cut(s)	65, 784
Bsp1407I	TGTACA	2 cut(s)	2239, 2336
Bsp1720I	GCTNAGC	1 cut(s)	1976
BspACI	CCGC	7 cut(s)	481, 1001, 1243, 1787, 2151, 2285, 2630
BspANI	GGCC	9 cut(s)	335, 484, 1021, 1075, 1582, 2225, 2621, 2640, 2691
BspCNI	CTCAG	6 cut(s)	557, 972, 1005, 1051, 1607, 2564
BspDI	ATCGAT	1 cut(s)	385
BspFNI	CGCG	1 cut(s)	730
BspLI	GGNNCC	3 cut(s)	312, 1408, 2328
BspMI	ACCTGC	1 cut(s)	2035
BspOI	GCTAGC	1 cut(s)	2147
BspPI	GGATC	5 cut(s)	390, 1401, 1414, 1935, 2295
BspT107I	GGYRCC	1 cut(s)	310
BsrDI	GCAATG	2 cut(s)	371, 2538
BsrFI	RCCGGY	2 cut(s)	2546, 2596
BsrGI	TGTACA	2 cut(s)	2239, 2336
BsrI	ACTGG	2 cut(s)	31, 314
BssAI	RCCGGY	2 cut(s)	2546, 2596
BssECI	CCNNGG	7 cut(s)	164, 210, 268, 277, 567, 591, 1305
BssNAI	GTATAC	1 cut(s)	849
BssNI	GRCGYC	1 cut(s)	1431
BssT1I	CCWWGG	4 cut(s)	164, 268, 591, 1305
Bst1107I	GTATAC	1 cut(s)	849
Bst2UI	CCWGG	5 cut(s)	212, 278, 1428, 2642, 2741
Bst4CI	ACNGT	5 cut(s)	1252, 1419, 1997, 2244, 2362
Bst6I	CTCTTC	1 cut(s)	855
BstACI	GRCGYC	1 cut(s)	1431
BstAPI	GCANNNNNTGC	1 cut(s)	1009
BstAUI	TGTACA	2 cut(s)	2239, 2336
BstC8I	GCNNGC	5 cut(s)	410, 1800, 2145, 2149, 2598
BstFNI	CGCG	1 cut(s)	730
BstHHI	GCGC	1 cut(s)	730
BstMAI	GTCTC	5 cut(s)	154, 523, 793, 976, 2549
BstMWI	GCNNNNNNNGC	5 cut(s)	942, 1009, 1018, 1060, 1588
BstNI	CCWGG	5 cut(s)	212, 278, 1428, 2642, 2741
BstNSI	RCATGY	1 cut(s)	2448
BstSCI	CCNGG	5 cut(s)	210, 276, 1426, 2640, 2739
BstSFI	CTRYAG	2 cut(s)	1094, 1947
BstUI	CGCG	1 cut(s)	730
BstV1I	GCAGC	6 cut(s)	737, 1018, 1157, 1241, 2644, 2698
BstX2I	RGATCY	2 cut(s)	1406, 1804
BstYI	RGATCY	2 cut(s)	1406, 1804
BstZ17I	GTATAC	1 cut(s)	849
Bsu15I	ATCGAT	1 cut(s)	385
BsuI	GTATCC	1 cut(s)	2638
BsuRI	GGCC	9 cut(s)	335, 484, 1021, 1075, 1582, 2225, 2621, 2640, 2691
BsuTUI	ATCGAT	1 cut(s)	385
BtsIMutI	CAGTG	3 cut(s)	258, 555, 2420
BveI	ACCTGC	1 cut(s)	2035
Cac8I	GCNNGC	5 cut(s)	410, 1800, 2145, 2149, 2598
CaiI	CAGNNNCTG	3 cut(s)	551, 1490, 2528
CfoI	GCGC	1 cut(s)	730
Cfr10I	RCCGGY	2 cut(s)	2546, 2596
Cfr13I	GGNCC	3 cut(s)	333, 875, 2109
ClaI	ATCGAT	1 cut(s)	385
CseI	GACGC	2 cut(s)	879, 1420
Csp6I	GTAC	7 cut(s)	7, 311, 2072, 2208, 2240, 2337, 2608
CspCI	CAANNNNNGTGG	2 cut(s)	2689, 2724
CviAII	CATG	8 cut(s)	17, 489, 515, 703, 832, 1323, 2093, 2445
CviQI	GTAC	7 cut(s)	7, 311, 2072, 2208, 2240, 2337, 2608
DraI	TTTAAA	1 cut(s)	1543
DriI	GACNNNNNGTC	1 cut(s)	1430
EaeI	YGGCCR	3 cut(s)	1019, 2638, 2689
Eam1104I	CTCTTC	1 cut(s)	855
Eam1105I	GACNNNNNGTC	1 cut(s)	1430
EarI	CTCTTC	1 cut(s)	855
EciI	GGCGGA	1 cut(s)	1802
Eco130I	CCWWGG	4 cut(s)	164, 268, 591, 1305
Eco32I	GATATC	1 cut(s)	1936
Eco47I	GGWCC	2 cut(s)	875, 2109
Eco57I	CTGAAG	1 cut(s)	2444
Eco88I	CYCGRG	2 cut(s)	566, 2454
EcoRI	GAATTC	1 cut(s)	1813
EcoRII	CCWGG	5 cut(s)	210, 276, 1426, 2640, 2739
EcoRV	GATATC	1 cut(s)	1936
EcoT14I	CCWWGG	4 cut(s)	164, 268, 591, 1305
EcoT22I	ATGCAT	1 cut(s)	400
ErhI	CCWWGG	4 cut(s)	164, 268, 591, 1305
FaeI	CATG	8 cut(s)	20, 492, 518, 706, 835, 1326, 2096, 2448
FaqI	GGGAC	2 cut(s)	840, 867
FatI	CATG	8 cut(s)	16, 488, 514, 702, 831, 1322, 2092, 2444
FauI	CCCGC	2 cut(s)	2144, 2637
FblI	GTMKAC	3 cut(s)	848, 1711, 2574
Fnu4HI	GCNGC	7 cut(s)	482, 726, 1032, 1146, 1230, 2658, 2712
Fsp4HI	GCNGC	7 cut(s)	482, 726, 1032, 1146, 1230, 2658, 2712
GlaI	GCGC	1 cut(s)	729
GluI	GCNGC	7 cut(s)	482, 726, 1032, 1146, 1230, 2658, 2712
GsuI	CTGGAG	1 cut(s)	657
HaeIII	GGCC	9 cut(s)	335, 484, 1021, 1075, 1582, 2225, 2621, 2640, 2691
HapII	CCGG	5 cut(s)	157, 380, 2471, 2547, 2597
HgaI	GACGC	2 cut(s)	879, 1420
HhaI	GCGC	1 cut(s)	730
Hin1I	GRCGYC	1 cut(s)	1431
Hin1II	CATG	8 cut(s)	20, 492, 518, 706, 835, 1326, 2096, 2448
Hin6I	GCGC	1 cut(s)	728
HinP1I	GCGC	1 cut(s)	728
HincII	GTYRAC	2 cut(s)	46, 2442
HindII	GTYRAC	2 cut(s)	46, 2442
HindIII	AAGCTT	1 cut(s)	2426
HpaII	CCGG	5 cut(s)	157, 380, 2471, 2547, 2597
HphI	GGTGA	1 cut(s)	1097
Hpy166II	GTNNAC	6 cut(s)	46, 849, 1712, 2240, 2442, 2575
Hpy8I	GTNNAC	6 cut(s)	46, 849, 1712, 2240, 2442, 2575
HpyAV	CCTTC	7 cut(s)	80, 452, 495, 1700, 1777, 2027, 2317
HpyCH4III	ACNGT	5 cut(s)	1252, 1419, 1997, 2244, 2362
HpyCH4IV	ACGT	1 cut(s)	1138
HpyF10VI	GCNNNNNNNGC	5 cut(s)	942, 1009, 1018, 1060, 1588
HpySE526I	ACGT	1 cut(s)	1138
Hsp92I	GRCGYC	1 cut(s)	1431
Hsp92II	CATG	8 cut(s)	20, 492, 518, 706, 835, 1326, 2096, 2448
HspAI	GCGC	1 cut(s)	728
KpnI	GGTACC	1 cut(s)	314
KroI	GCCGGC	1 cut(s)	2596
KroNI	GCCGGC	1 cut(s)	2598
LmnI	GCTCC	2 cut(s)	676, 1174
Lsp1109I	GCAGC	6 cut(s)	737, 1018, 1157, 1241, 2644, 2698
LweI	GCATC	5 cut(s)	385, 545, 631, 2414, 2644
MaeII	ACGT	1 cut(s)	1138
MaeIII	GTNAC	6 cut(s)	1907, 2024, 2056, 2315, 2489, 2534
MfeI	CAATTG	1 cut(s)	841
MflI	RGATCY	2 cut(s)	1406, 1804
MhlI	GDGCHC	2 cut(s)	65, 784
MlsI	TGGCCA	3 cut(s)	1021, 2640, 2691
MluNI	TGGCCA	3 cut(s)	1021, 2640, 2691
MlyI	GAGTC	6 cut(s)	107, 156, 539, 1562, 1904, 2466
MmeI	TCCRAC	3 cut(s)	351, 1703, 1970
Mox20I	TGGCCA	3 cut(s)	1021, 2640, 2691
Mph1103I	ATGCAT	1 cut(s)	400
MroNI	GCCGGC	1 cut(s)	2596
MroXI	GAANNNNTTC	2 cut(s)	1506, 1734
MscI	TGGCCA	3 cut(s)	1021, 2640, 2691
MslI	CAYNNNNRTG	5 cut(s)	261, 403, 1261, 2496, 2498
Msp20I	TGGCCA	3 cut(s)	1021, 2640, 2691
MspA1I	CMGCKG	2 cut(s)	2151, 2285
MspI	CCGG	5 cut(s)	157, 380, 2471, 2547, 2597
MspR9I	CCNGG	5 cut(s)	212, 278, 1428, 2642, 2741
MunI	CAATTG	1 cut(s)	841
Mva1269I	GAATGC	2 cut(s)	410, 2273
MvaI	CCWGG	5 cut(s)	212, 278, 1428, 2642, 2741
MvnI	CGCG	1 cut(s)	730
MwoI	GCNNNNNNNGC	5 cut(s)	942, 1009, 1018, 1060, 1588
NaeI	GCCGGC	1 cut(s)	2598
NgoMIV	GCCGGC	1 cut(s)	2596
NheI	GCTAGC	1 cut(s)	2143
NlaIII	CATG	8 cut(s)	20, 492, 518, 706, 835, 1326, 2096, 2448
NlaIV	GGNNCC	3 cut(s)	312, 1408, 2328
NmuCI	GTSAC	2 cut(s)	1907, 2489
NsiI	ATGCAT	1 cut(s)	400
NspI	RCATGY	1 cut(s)	2448
OliI	CACNNNNGTG	1 cut(s)	2498
PaqCI	CACCTGC	1 cut(s)	2035
PctI	GAATGC	2 cut(s)	410, 2273
PdiI	GCCGGC	1 cut(s)	2598
PdmI	GAANNNNTTC	2 cut(s)	1506, 1734
PfeI	GAWTC	9 cut(s)	160, 186, 215, 548, 560, 734, 1394, 2255, 2392
PflFI	GACNNNGTC	1 cut(s)	970
PflMI	CCANNNNNTGG	1 cut(s)	342
PfoI	TCCNGGA	1 cut(s)	2739
PkrI	GCNGC	7 cut(s)	483, 727, 1033, 1147, 1231, 2659, 2713
PleI	GAGTC	6 cut(s)	106, 155, 538, 1561, 1904, 2465
PpsI	GAGTC	6 cut(s)	106, 155, 538, 1561, 1904, 2465
PshBI	ATTAAT	1 cut(s)	740
Psp6I	CCWGG	5 cut(s)	210, 276, 1426, 2640, 2739
PspGI	CCWGG	5 cut(s)	210, 276, 1426, 2640, 2739
PspN4I	GGNNCC	3 cut(s)	312, 1408, 2328
PspPI	GGNCC	3 cut(s)	333, 875, 2109
PstNI	CAGNNNCTG	3 cut(s)	551, 1490, 2528
PsuI	RGATCY	2 cut(s)	1406, 1804
PsyI	GACNNNGTC	1 cut(s)	970
RsaI	GTAC	7 cut(s)	8, 312, 2073, 2209, 2241, 2338, 2609
RsaNI	GTAC	7 cut(s)	7, 311, 2072, 2208, 2240, 2337, 2608
RseI	CAYNNNNRTG	5 cut(s)	261, 403, 1261, 2496, 2498
SatI	GCNGC	7 cut(s)	482, 726, 1032, 1146, 1230, 2658, 2712
Sau96I	GGNCC	3 cut(s)	333, 875, 2109
ScaI	AGTACT	1 cut(s)	2073
SchI	GAGTC	6 cut(s)	107, 156, 539, 1562, 1904, 2466
ScrFI	CCNGG	5 cut(s)	212, 278, 1428, 2642, 2741
SduI	GDGCHC	2 cut(s)	65, 784
SfaNI	GCATC	5 cut(s)	385, 545, 631, 2414, 2644
SfcI	CTRYAG	2 cut(s)	1094, 1947
SinI	GGWCC	2 cut(s)	875, 2109
SmiMI	CAYNNNNRTG	5 cut(s)	261, 403, 1261, 2496, 2498
SmlI	CTYRAG	3 cut(s)	358, 946, 1739
SmoI	CTYRAG	3 cut(s)	358, 946, 1739
SpeI	ACTAGT	1 cut(s)	851
SsiI	CCGC	7 cut(s)	481, 1001, 1243, 1787, 2151, 2285, 2630
SspI	AATATT	2 cut(s)	1378, 1899
StyD4I	CCNGG	5 cut(s)	210, 276, 1426, 2640, 2739
StyI	CCWWGG	4 cut(s)	164, 268, 591, 1305
TaaI	ACNGT	5 cut(s)	1252, 1419, 1997, 2244, 2362
TaiI	ACGT	1 cut(s)	1141
TaqI	TCGA	4 cut(s)	385, 528, 988, 2321
TatI	WGTACW	3 cut(s)	2071, 2239, 2336
TauI	GCSGC	1 cut(s)	484
TfiI	GAWTC	9 cut(s)	160, 186, 215, 548, 560, 734, 1394, 2255, 2392
TscAI	CASTG	3 cut(s)	265, 562, 2427
TseFI	GTSAC	2 cut(s)	1907, 2489
TseI	GCWGC	6 cut(s)	725, 1031, 1145, 1229, 2657, 2711
Tsp45I	GTSAC	2 cut(s)	1907, 2489
TspRI	CASTG	3 cut(s)	265, 562, 2427
Tth111I	GACNNNGTC	1 cut(s)	970
Van91I	CCANNNNNTGG	1 cut(s)	342
VpaK11BI	GGWCC	2 cut(s)	875, 2109
VspI	ATTAAT	1 cut(s)	740
XapI	RAATTY	7 cut(s)	652, 1103, 1193, 1813, 1859, 2295, 2716
XbaI	TCTAGA	2 cut(s)	125, 179
XceI	RCATGY	1 cut(s)	2448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1442
XmaJI	CCTAGG	1 cut(s)	268
XmiI	GTMKAC	3 cut(s)	848, 1711, 2574
XmnI	GAANNNNTTC	2 cut(s)	1506, 1734
ZrmI	AGTACT	1 cut(s)	2073
Zsp2I	ATGCAT	1 cut(s)	400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.