Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0000964

Physical Location & Seq

Genomic Coordinates Reverse (-)

28019752 .. 28024661

Gene Structure 4910 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG232000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGTGTGTGTTATTGCCTTCATGCTGCTATAGGAGGATAATAATGGAAGGCTCATCTGAGTCTGCTTGGCAGAGGTCTAATAGTTCTAGAGATTTGAATACTTCCAGAGTCTCAAACCGGAATCCAAGGCGTAGTCATTCTAGAAGATTCGGCTTCTTGCGTGGTTCATCGCAGGATTCGGATTTAAGGAATGAGAGAGATGGGGTTGTGGTGGCACACACTGATGACCTAGGAAACCAGGGTGGATTGTCTGGGGTGTGTGAGGATGAGGTACCAGTTGAACCTTTTGTTCGGGCCATTGAATGGGGAGATGTTAGCTTGAGGCAATGGTTGGATAGACCGGATCGATCTGTTGATGCATTTGAATGCTTGCATATATTTAGGCAAATAGTGGAAATTGTTAATGTAGCACATTCAGAAGGAATTGTTGTTCATAATGTGCGGCCTTCCTGCTTTGTTATGTCCTCTTTTAATCATGTCTCTTTCATCGAGTCTGCATCTTGCTCAGATTCTGGCACTGATTCTCCCGAGGATGGGTTAAATAGCCCAACCCTGGAAGCAAAGAACTTAACCTCTGCCTTGCATCAGAAACGAAGCAACATGGCAAGAGGAAATTTTCAATTTATGAAAGCTCCAGCAAATGCTTTGTCAGATACAGGTTGCATGCAGTCAAGCTCAATATATGCGGCGCGTGAATCATTAATGCAAGAGAGTGAAGAACATAGAACCAGAGAGAGGAGTGCTCAACTAGAAGATAAGAGACAGCCATTTCCAATGAAACAAATATTGCTCATGGAGAGCAATTGGTATACTAGTCCTGAAGAGGTTGCTGGTGGTCCAAGTCCCTGTGCTTCAGACATTTATCGATTAGGAGTTCTTCTTTTTGAGTTATTCTGCCCATTCAGCTCAAGAGAAGAAAAGAGTAGGACCATGTCTAGTCTGAGACATCGAGTTCTACCTCCGCAATTACTGCTGAGATGGCCAAAAGAAGCTTCATTTTGCTTATGGCTACTGCACCCTGAGCCAAATAGTCGGCCTAAAATGGGTGAATTGCTACAGAGTGAATTTCTTAATGAACCAAGAGATGATTTAGAAGAACGTGAAGCAGCAATAGAGCTTAGAGAGAAAATAGAGGAGCAGGAGTTATTGCTGGAATTTCTCTTGCTTGTAAAACAAAGGAAACAGGAAGCAGCTGATAAGTTGCGGAATACTGTTTCATTTCTGTGTTCTGATATTGAAGAAGTTGTGAAGCACAAAACAAGTTCCAAGGGAAAAGGTGGTTCATGCCCAGAGCTGGTAAAGGAAGATCATTCAACATCAAGTTTCCCATCTATGAATATTACTGATGATGATGATTCGGCTAGTGGATCCAGAAAACGGTTCAGACCAGGCGTCTATATTCACAATGGAGAGGAGTGTGATGATAATTTGGATGGTCAGAAGTCAGAAACTGATAATCAAGAAAGTATTCTTTTGAGAAGTTCCAGATTGATGAACAACTTTAAAAAGCTAGAGTCAGCATATTTTTTGACAAGATGGAGGCCAATCAAGCACTCAGCCAAACCCTTGACTAGACATTCATCAATAAGTAGTGATGGTAGAGGTTCTATTGTTGCAACTGAAAGAAGTTCTGTGAGTAATTTGACATTAAAAGAACAACATAGTGAAGGTAGACGAAGTGGTTGGATAGAACCATTTCTTGAGGGATTATGCAAGTATCTATCTTTCTCAAAATTAAAAGTGAAGGCGGATTTGAAGCAGGCAGATCTTCTGAATTCCTCAAATCTAGTATGCTCACTTAGTTTTGATCGGGATGGAGAATTTTTTGCTACAGCTGGTGTTAATAAGAAAATCAAAATATTTGAATGTGACTCGATCATAAACGAGGATCGTGATATCCATTATCCTGTGGTTGAAATAGCTACCAGATCAAAGCTAAGCAATATATGTTGGAACAGTTATATCAAAAGCCAGATTGCTTCAAGTAACTTTGAAGGTGTAGTGCAGGTGTGGGATGTTACAAGAAGTCAAGTACTAATGGAGATGAAAGAACATGAGAAGCGTGTATGGTCCATTGACTTTTCATCAGCAGACCCAACAATGCTAGCTAGCGGGAGTGATGATGGTTCTGTTAAGCTATGGAGTATCAATCAGGGAGAAAGTATTGGTTCAATAAAAACAAAGGCCAATGTTTGCTGTGTACAGTTTCCTTTGGATTCTTGTCGTTCCCTTGCATTCGGTTCAGCGGATCATAAAATTTATTACTATGATCTTCGTAACTCGAAGGTTCCTCTTTGTACATTGATTGGACACAACAAAACTGTGAGTTATGTCAAGTTCATAGATAGGATGAATCTTGTTTCTGCATCCACCGACAACACACTGAAGCTTTGGGATCTGTCAACATGCACATCTCGAGTCATAGACACTCCGGTTATGACATTCACAGGTCACACGAATGTGAAGAACTTTGTGGGTTTGTCAGTTTCTGATGGTTACATTGCTACCGGCTCAGAGACTAATGAGGTTTTCGTCTACCACAAGGCATTCCCAATGCCGGCGTTGTCGTACAAGTTTCAGGCCACAGACCCGCTTTCTGGCCAGGATACGGATGATGCTGCACAATTTATCTCATCTGTTTGTTGGCGTGGCCAATCCTCCACCTTAATTGCTGCAAATTCCACAGGGAATATCAAAATCCTGGAGATGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0031461 GO:0032991 GO:0042802 GO:0044424 GO:0044464 GO:0050896 GO:0080008 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04115 ko04120 ko04712 map04115 map04120 map04712

Pfam Domains

Protein Families

Pkinase WD40

Protein Analysis

904

Amino Acids

101.59

Weight (kDa)

6.0

Isoelectric Point (pI)

62.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	588 - 728	8e-13	WDR3 first beta-propeller domain
Beta-prop_WDR19_1st	PF23389	590 - 736	5.7e-06	WDR19 first beta-propeller
Beta-prop_WDR3_2nd	PF25172	592 - 799	4.5e-13	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	594 - 847	9e-31	WDR5 beta-propeller domain
WDR55	PF24796	594 - 721	4.1e-07	WDR55
WD40_CDC20-Fz	PF24807	643 - 736	8.6e-14	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	644 - 843	2.2e-24	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	657 - 805	1.4e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	657 - 726	1.8e-06	WDHD1 first WD40 domain
WD40_Prp19	PF24814	661 - 736	8.7e-10	Prp19 WD40 domain
WD40_MABP1-WDR62_1st	PF24780	662 - 761	7.7e-06	MABP1/WDR62 first WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	662 - 798	4.4e-16	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	664 - 803	1e-05	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	664 - 846	5.6e-26	WDR3 first beta-propeller domain
WD40	PF00400	678 - 715	1.7e-07	WD domain, G-beta repeat
EIF3I	PF24805	685 - 740	1.6e-06	EIF3I
Beta-prop_WDR3_2nd	PF25172	696 - 816	1.5e-09	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	696 - 848	1.1e-06	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	701 - 837	1.6e-08	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	726 - 804	4.4e-07	WDHD1 first WD40 domain
WD40_Prp19	PF24814	727 - 839	1.5e-08	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	728 - 820	1.3e-07	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	742 - 839	7.7e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	742 - 902	7.2e-10	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT1G53090 AT3G15354 AT3G15354 AT3G15354 AT3G15354 AT3G15354
fragaria_vesca	FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920 FvH4_3g19920
malus_domestica	MD03G1228100.v1.1 MD11G1247500.v1.1
prunus_persica	Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1 Prupe.4G180900_v2.0.a1
pyrus_communis	pycom03g17550 pycom11g22000
rosa_chinensis	RchiOBHm_Chr5g0033591
rosa_laevigata	RLG00000033491
rosa_roxburghii	Rroxscaffold_1G00046490
rosa_rugosa	Rorug05G0140300 Rorug05G0140400 Rorug05G0140500 Rorug05G0140600 Rorug05G0140700
rosa_samantha	Rh5AG232000 Rh5BG232700 Rh5CG261600 Rh5DG238300
rosa_wichuraiana	Rw5G021170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1996
Acc36I	ACCTGC	1 cut(s)	1996
Acc65I	GGTACC	1 cut(s)	271
AccB1I	GGYRCC	1 cut(s)	271
AccB7I	CCANNNNNTGG	1 cut(s)	303
AccI	GTMKAC	3 cut(s)	809, 1672, 2535
AccII	CGCG	1 cut(s)	691
AciI	CCGC	8 cut(s)	442, 686, 962, 1204, 1748, 2112, 2246, 2591
AclWI	GGATC	6 cut(s)	351, 1362, 1375, 1896, 2256, 2403
AcoI	YGGCCR	3 cut(s)	980, 2599, 2650
AcsI	RAATTY	7 cut(s)	613, 1064, 1154, 1774, 1820, 2256, 2677
AcuI	CTGAAG	3 cut(s)	837, 840, 2405
AcyI	GRCGYC	1 cut(s)	1392
AfaI	GTAC	5 cut(s)	273, 2034, 2202, 2299, 2570
AhdI	GACNNNNNGTC	1 cut(s)	1391
AhlI	ACTAGT	1 cut(s)	812
AjnI	CCWGG	5 cut(s)	237, 552, 1387, 2601, 2700
AjuI	GAANNNNNNNTTGG	2 cut(s)	976, 1008
AleI	CACNNNNGTG	1 cut(s)	2459
AloI	GAACNNNNNNTCC	2 cut(s)	414, 446
Alw21I	GWGCWC	1 cut(s)	745
Alw26I	GTCTC	5 cut(s)	115, 484, 754, 937, 2510
AlwI	GGATC	6 cut(s)	351, 1362, 1375, 1896, 2256, 2403
AlwNI	CAGNNNCTG	3 cut(s)	512, 1451, 2489
Ama87I	CYCGRG	2 cut(s)	527, 2415
AoxI	GGCC	9 cut(s)	294, 443, 980, 1034, 1541, 2184, 2580, 2599, 2650
ApeKI	GCWGC	5 cut(s)	24, 1106, 1190, 2618, 2672
ApoI	RAATTY	7 cut(s)	613, 1064, 1154, 1774, 1820, 2256, 2677
ArsI	GACNNNNNNTTYG	2 cut(s)	632, 664
AseI	ATTAAT	1 cut(s)	701
Asp700I	GAANNNNTTC	2 cut(s)	1467, 1695
Asp718I	GGTACC	1 cut(s)	271
AspA2I	CCTAGG	1 cut(s)	229
AspLEI	GCGC	1 cut(s)	691
AspS9I	GGNCC	4 cut(s)	294, 836, 927, 2070
AsuHPI	GGTGA	1 cut(s)	1058
AsuNHI	GCTAGC	2 cut(s)	2104, 2108
AvaI	CYCGRG	2 cut(s)	527, 2415
AvaII	GGWCC	3 cut(s)	836, 927, 2070
AvrII	CCTAGG	1 cut(s)	229
BalI	TGGCCA	3 cut(s)	982, 2601, 2652
BamHI	GGATCC	1 cut(s)	1367
BanI	GGYRCC	1 cut(s)	271
Bbv12I	GWGCWC	1 cut(s)	745
BbvI	GCAGC	5 cut(s)	11, 1118, 1202, 2605, 2659
BciT130I	CCWGG	5 cut(s)	239, 554, 1389, 2603, 2702
BciVI	GTATCC	1 cut(s)	2599
BcoDI	GTCTC	5 cut(s)	115, 484, 754, 937, 2510
BcuI	ACTAGT	1 cut(s)	812
BfmI	CTRYAG	3 cut(s)	28, 1055, 1830
BfuAI	ACCTGC	1 cut(s)	1996
BfuI	GTATCC	1 cut(s)	2599
BglII	AGATCT	1 cut(s)	1765
BisI	GCNGC	7 cut(s)	25, 443, 687, 1107, 1191, 2619, 2673
BlnI	CCTAGG	1 cut(s)	229
BlpI	GCTNAGC	1 cut(s)	1937
BlsI	GCNGC	7 cut(s)	26, 444, 688, 1108, 1192, 2620, 2674
BmcAI	AGTACT	1 cut(s)	2034
Bme1390I	CCNGG	5 cut(s)	239, 554, 1389, 2603, 2702
Bme18I	GGWCC	3 cut(s)	836, 927, 2070
BmeRI	GACNNNNNGTC	1 cut(s)	1391
BmeT110I	CYCGRG	2 cut(s)	527, 2415
BmgT120I	GGNCC	4 cut(s)	294, 836, 927, 2070
BmiI	GGNNCC	3 cut(s)	273, 1369, 2289
BmrFI	CCNGG	5 cut(s)	239, 554, 1389, 2603, 2702
BmsI	GCATC	5 cut(s)	346, 506, 592, 2375, 2605
BmtI	GCTAGC	2 cut(s)	2108, 2112
BplI	GAGNNNNNCTC	2 cut(s)	727, 759
BpmI	CTGGAG	1 cut(s)	618
Bpu10I	CCTNAGC	1 cut(s)	1020
Bpu1102I	GCTNAGC	1 cut(s)	1937
BpuEI	CTTGAG	3 cut(s)	340, 892, 1721
Bsa29I	ATCGAT	2 cut(s)	346, 865
BsaHI	GRCGYC	1 cut(s)	1392
BsaJI	CCNNGG	6 cut(s)	125, 229, 238, 528, 552, 1266
BsaWI	WCCGGW	3 cut(s)	117, 340, 2431
BsaXI	ACNNNNNCTCC	4 cut(s)	508, 538, 1127, 1157
Bse118I	RCCGGY	2 cut(s)	2507, 2557
Bse1I	ACTGG	1 cut(s)	275
Bse3DI	GCAATG	2 cut(s)	332, 2499
BseBI	CCWGG	5 cut(s)	239, 554, 1389, 2603, 2702
BseCI	ATCGAT	2 cut(s)	346, 865
BseDI	CCNNGG	6 cut(s)	125, 229, 238, 528, 552, 1266
BseGI	GGATG	8 cut(s)	271, 538, 1438, 1819, 2020, 2355, 2366, 2617
BseMI	GCAATG	2 cut(s)	332, 2499
BseMII	CTCAG	7 cut(s)	48, 519, 932, 965, 1011, 1569, 2526
BseNI	ACTGG	1 cut(s)	275
BseRI	GAGGAG	3 cut(s)	751, 1148, 1427
BseXI	GCAGC	5 cut(s)	11, 1118, 1202, 2605, 2659
BsgI	GTGCAG	3 cut(s)	998, 2024, 2604
Bsh1236I	CGCG	1 cut(s)	691
BshFI	GGCC	9 cut(s)	296, 445, 982, 1036, 1543, 2186, 2582, 2601, 2652
BshNI	GGYRCC	1 cut(s)	271
BshVI	ATCGAT	2 cut(s)	346, 865
BsiHKAI	GWGCWC	1 cut(s)	745
BsiHKCI	CYCGRG	2 cut(s)	527, 2415
BsiSI	CCGG	5 cut(s)	118, 341, 2432, 2508, 2558
BslFI	GGGAC	1 cut(s)	828
BsmAI	GTCTC	5 cut(s)	115, 484, 754, 937, 2510
BsmFI	GGGAC	1 cut(s)	828
BsmI	GAATGC	3 cut(s)	371, 2234, 2546
BsnI	GGCC	9 cut(s)	296, 445, 982, 1036, 1543, 2186, 2582, 2601, 2652
BsoBI	CYCGRG	2 cut(s)	527, 2415
Bsp1286I	GDGCHC	1 cut(s)	745
Bsp1407I	TGTACA	2 cut(s)	2200, 2297
Bsp1720I	GCTNAGC	1 cut(s)	1937
BspACI	CCGC	8 cut(s)	442, 686, 962, 1204, 1748, 2112, 2246, 2591
BspANI	GGCC	9 cut(s)	296, 445, 982, 1036, 1543, 2186, 2582, 2601, 2652
BspCNI	CTCAG	7 cut(s)	49, 518, 933, 966, 1012, 1568, 2525
BspDI	ATCGAT	2 cut(s)	346, 865
BspFNI	CGCG	1 cut(s)	691
BspLI	GGNNCC	3 cut(s)	273, 1369, 2289
BspMI	ACCTGC	1 cut(s)	1996
BspOI	GCTAGC	2 cut(s)	2108, 2112
BspPI	GGATC	6 cut(s)	351, 1362, 1375, 1896, 2256, 2403
BspT107I	GGYRCC	1 cut(s)	271
BsrDI	GCAATG	2 cut(s)	332, 2499
BsrFI	RCCGGY	2 cut(s)	2507, 2557
BsrGI	TGTACA	2 cut(s)	2200, 2297
BsrI	ACTGG	1 cut(s)	275
BssAI	RCCGGY	2 cut(s)	2507, 2557
BssECI	CCNNGG	6 cut(s)	125, 229, 238, 528, 552, 1266
BssNAI	GTATAC	1 cut(s)	810
BssNI	GRCGYC	1 cut(s)	1392
BssT1I	CCWWGG	3 cut(s)	125, 229, 1266
Bst1107I	GTATAC	1 cut(s)	810
Bst2UI	CCWGG	5 cut(s)	239, 554, 1389, 2603, 2702
Bst4CI	ACNGT	5 cut(s)	1213, 1380, 1958, 2205, 2323
Bst6I	CTCTTC	1 cut(s)	816
BstACI	GRCGYC	1 cut(s)	1392
BstAPI	GCANNNNNTGC	1 cut(s)	970
BstAUI	TGTACA	2 cut(s)	2200, 2297
BstC8I	GCNNGC	6 cut(s)	371, 665, 1761, 2106, 2110, 2559
BstF5I	GGATG	8 cut(s)	271, 538, 1438, 1819, 2020, 2355, 2366, 2617
BstFNI	CGCG	1 cut(s)	691
BstHHI	GCGC	1 cut(s)	691
BstMAI	GTCTC	5 cut(s)	115, 484, 754, 937, 2510
BstMWI	GCNNNNNNNGC	5 cut(s)	903, 970, 979, 1021, 1549
BstNI	CCWGG	5 cut(s)	239, 554, 1389, 2603, 2702
BstNSI	RCATGY	2 cut(s)	667, 2409
BstSCI	CCNGG	5 cut(s)	237, 552, 1387, 2601, 2700
BstSFI	CTRYAG	3 cut(s)	28, 1055, 1830
BstUI	CGCG	1 cut(s)	691
BstV1I	GCAGC	5 cut(s)	11, 1118, 1202, 2605, 2659
BstX2I	RGATCY	3 cut(s)	1367, 1765, 2395
BstYI	RGATCY	3 cut(s)	1367, 1765, 2395
BstZ17I	GTATAC	1 cut(s)	810
Bsu15I	ATCGAT	2 cut(s)	346, 865
BsuI	GTATCC	1 cut(s)	2599
BsuRI	GGCC	9 cut(s)	296, 445, 982, 1036, 1543, 2186, 2582, 2601, 2652
BsuTUI	ATCGAT	2 cut(s)	346, 865
BtgZI	GCGATG	1 cut(s)	153
BtsCI	GGATG	8 cut(s)	271, 538, 1438, 1819, 2020, 2355, 2366, 2617
BtsIMutI	CAGTG	3 cut(s)	219, 516, 2381
BveI	ACCTGC	1 cut(s)	1996
Cac8I	GCNNGC	6 cut(s)	371, 665, 1761, 2106, 2110, 2559
CaiI	CAGNNNCTG	3 cut(s)	512, 1451, 2489
CfoI	GCGC	1 cut(s)	691
Cfr10I	RCCGGY	2 cut(s)	2507, 2557
Cfr13I	GGNCC	4 cut(s)	294, 836, 927, 2070
ClaI	ATCGAT	2 cut(s)	346, 865
CseI	GACGC	1 cut(s)	1381
Csp6I	GTAC	5 cut(s)	272, 2033, 2201, 2298, 2569
CspCI	CAANNNNNGTGG	2 cut(s)	2650, 2685
CviAII	CATG	9 cut(s)	21, 476, 601, 664, 793, 931, 1284, 2054, 2406
CviQI	GTAC	5 cut(s)	272, 2033, 2201, 2298, 2569
DraI	TTTAAA	1 cut(s)	1504
DriI	GACNNNNNGTC	1 cut(s)	1391
EaeI	YGGCCR	3 cut(s)	980, 2599, 2650
Eam1104I	CTCTTC	1 cut(s)	816
Eam1105I	GACNNNNNGTC	1 cut(s)	1391
EarI	CTCTTC	1 cut(s)	816
EciI	GGCGGA	1 cut(s)	1763
Eco130I	CCWWGG	3 cut(s)	125, 229, 1266
Eco32I	GATATC	1 cut(s)	1897
Eco47I	GGWCC	3 cut(s)	836, 927, 2070
Eco57I	CTGAAG	3 cut(s)	837, 840, 2405
Eco88I	CYCGRG	2 cut(s)	527, 2415
EcoRI	GAATTC	1 cut(s)	1774
EcoRII	CCWGG	5 cut(s)	237, 552, 1387, 2601, 2700
EcoRV	GATATC	1 cut(s)	1897
EcoT14I	CCWWGG	3 cut(s)	125, 229, 1266
EcoT22I	ATGCAT	1 cut(s)	361
ErhI	CCWWGG	3 cut(s)	125, 229, 1266
FaeI	CATG	9 cut(s)	24, 479, 604, 667, 796, 934, 1287, 2057, 2409
FaqI	GGGAC	1 cut(s)	828
FatI	CATG	9 cut(s)	20, 475, 600, 663, 792, 930, 1283, 2053, 2405
FauI	CCCGC	2 cut(s)	2105, 2598
FblI	GTMKAC	3 cut(s)	809, 1672, 2535
Fnu4HI	GCNGC	7 cut(s)	25, 443, 687, 1107, 1191, 2619, 2673
FokI	GGATG	8 cut(s)	278, 545, 1445, 1826, 2027, 2353, 2362, 2624
Fsp4HI	GCNGC	7 cut(s)	25, 443, 687, 1107, 1191, 2619, 2673
GlaI	GCGC	1 cut(s)	690
GluI	GCNGC	7 cut(s)	25, 443, 687, 1107, 1191, 2619, 2673
GsuI	CTGGAG	1 cut(s)	618
HaeIII	GGCC	9 cut(s)	296, 445, 982, 1036, 1543, 2186, 2582, 2601, 2652
HapII	CCGG	5 cut(s)	118, 341, 2432, 2508, 2558
HgaI	GACGC	1 cut(s)	1381
HhaI	GCGC	1 cut(s)	691
Hin1I	GRCGYC	1 cut(s)	1392
Hin1II	CATG	9 cut(s)	24, 479, 604, 667, 796, 934, 1287, 2057, 2409
Hin6I	GCGC	1 cut(s)	689
HinP1I	GCGC	1 cut(s)	689
HincII	GTYRAC	1 cut(s)	2403
HindII	GTYRAC	1 cut(s)	2403
HindIII	AAGCTT	2 cut(s)	990, 2387
HpaII	CCGG	5 cut(s)	118, 341, 2432, 2508, 2558
HphI	GGTGA	1 cut(s)	1058
Hpy166II	GTNNAC	5 cut(s)	810, 1673, 2201, 2403, 2536
Hpy8I	GTNNAC	5 cut(s)	810, 1673, 2201, 2403, 2536
HpyAV	CCTTC	8 cut(s)	27, 41, 413, 456, 1661, 1738, 1988, 2278
HpyCH4III	ACNGT	5 cut(s)	1213, 1380, 1958, 2205, 2323
HpyCH4IV	ACGT	1 cut(s)	1099
HpyF10VI	GCNNNNNNNGC	5 cut(s)	903, 970, 979, 1021, 1549
HpySE526I	ACGT	1 cut(s)	1099
Hsp92I	GRCGYC	1 cut(s)	1392
Hsp92II	CATG	9 cut(s)	24, 479, 604, 667, 796, 934, 1287, 2057, 2409
HspAI	GCGC	1 cut(s)	689
KpnI	GGTACC	1 cut(s)	275
KroI	GCCGGC	1 cut(s)	2557
KroNI	GCCGGC	1 cut(s)	2559
LmnI	GCTCC	2 cut(s)	637, 1135
Lsp1109I	GCAGC	5 cut(s)	11, 1118, 1202, 2605, 2659
LweI	GCATC	5 cut(s)	346, 506, 592, 2375, 2605
MaeII	ACGT	1 cut(s)	1099
MaeIII	GTNAC	6 cut(s)	1868, 1985, 2017, 2276, 2450, 2495
MfeI	CAATTG	1 cut(s)	802
MflI	RGATCY	3 cut(s)	1367, 1765, 2395
MhlI	GDGCHC	1 cut(s)	745
MlsI	TGGCCA	3 cut(s)	982, 2601, 2652
MluNI	TGGCCA	3 cut(s)	982, 2601, 2652
MlyI	GAGTC	6 cut(s)	68, 117, 500, 1523, 1865, 2427
MmeI	TCCRAC	3 cut(s)	312, 1664, 1931
Mox20I	TGGCCA	3 cut(s)	982, 2601, 2652
Mph1103I	ATGCAT	1 cut(s)	361
MroNI	GCCGGC	1 cut(s)	2557
MroXI	GAANNNNTTC	2 cut(s)	1467, 1695
MscI	TGGCCA	3 cut(s)	982, 2601, 2652
MslI	CAYNNNNRTG	5 cut(s)	222, 364, 1222, 2457, 2459
Msp20I	TGGCCA	3 cut(s)	982, 2601, 2652
MspA1I	CMGCKG	3 cut(s)	1193, 1835, 2246
MspI	CCGG	5 cut(s)	118, 341, 2432, 2508, 2558
MspR9I	CCNGG	5 cut(s)	239, 554, 1389, 2603, 2702
MunI	CAATTG	1 cut(s)	802
Mva1269I	GAATGC	3 cut(s)	371, 2234, 2546
MvaI	CCWGG	5 cut(s)	239, 554, 1389, 2603, 2702
MvnI	CGCG	1 cut(s)	691
MwoI	GCNNNNNNNGC	5 cut(s)	903, 970, 979, 1021, 1549
NaeI	GCCGGC	1 cut(s)	2559
NgoMIV	GCCGGC	1 cut(s)	2557
NheI	GCTAGC	2 cut(s)	2104, 2108
NlaIII	CATG	9 cut(s)	24, 479, 604, 667, 796, 934, 1287, 2057, 2409
NlaIV	GGNNCC	3 cut(s)	273, 1369, 2289
NmuCI	GTSAC	2 cut(s)	1868, 2450
NsiI	ATGCAT	1 cut(s)	361
NspI	RCATGY	2 cut(s)	667, 2409
OliI	CACNNNNGTG	1 cut(s)	2459
PaeI	GCATGC	1 cut(s)	667
PaeR7I	CTCGAG	1 cut(s)	2415
PaqCI	CACCTGC	1 cut(s)	1996
PctI	GAATGC	3 cut(s)	371, 2234, 2546
PdiI	GCCGGC	1 cut(s)	2559
PdmI	GAANNNNTTC	2 cut(s)	1467, 1695
PfeI	GAWTC	9 cut(s)	121, 147, 176, 509, 521, 695, 1355, 2216, 2353
PflFI	GACNNNGTC	1 cut(s)	931
PflMI	CCANNNNNTGG	1 cut(s)	303
PfoI	TCCNGGA	1 cut(s)	2700
PkrI	GCNGC	7 cut(s)	26, 444, 688, 1108, 1192, 2620, 2674
PleI	GAGTC	6 cut(s)	67, 116, 499, 1522, 1865, 2426
PpsI	GAGTC	6 cut(s)	67, 116, 499, 1522, 1865, 2426
PshBI	ATTAAT	1 cut(s)	701
Psp6I	CCWGG	5 cut(s)	237, 552, 1387, 2601, 2700
PspGI	CCWGG	5 cut(s)	237, 552, 1387, 2601, 2700
PspN4I	GGNNCC	3 cut(s)	273, 1369, 2289
PspPI	GGNCC	4 cut(s)	294, 836, 927, 2070
PstNI	CAGNNNCTG	3 cut(s)	512, 1451, 2489
PsuI	RGATCY	3 cut(s)	1367, 1765, 2395
PsyI	GACNNNGTC	1 cut(s)	931
PvuII	CAGCTG	2 cut(s)	1193, 1835
RsaI	GTAC	5 cut(s)	273, 2034, 2202, 2299, 2570
RsaNI	GTAC	5 cut(s)	272, 2033, 2201, 2298, 2569
RseI	CAYNNNNRTG	5 cut(s)	222, 364, 1222, 2457, 2459
SatI	GCNGC	7 cut(s)	25, 443, 687, 1107, 1191, 2619, 2673
Sau96I	GGNCC	4 cut(s)	294, 836, 927, 2070
ScaI	AGTACT	1 cut(s)	2034
SchI	GAGTC	6 cut(s)	68, 117, 500, 1523, 1865, 2427
ScrFI	CCNGG	5 cut(s)	239, 554, 1389, 2603, 2702
SduI	GDGCHC	1 cut(s)	745
SfaNI	GCATC	5 cut(s)	346, 506, 592, 2375, 2605
SfcI	CTRYAG	3 cut(s)	28, 1055, 1830
Sfr274I	CTCGAG	1 cut(s)	2415
SinI	GGWCC	3 cut(s)	836, 927, 2070
SlaI	CTCGAG	1 cut(s)	2415
SmiMI	CAYNNNNRTG	5 cut(s)	222, 364, 1222, 2457, 2459
SmlI	CTYRAG	4 cut(s)	319, 907, 1700, 2415
SmoI	CTYRAG	4 cut(s)	319, 907, 1700, 2415
SpeI	ACTAGT	1 cut(s)	812
SphI	GCATGC	1 cut(s)	667
SsiI	CCGC	8 cut(s)	442, 686, 962, 1204, 1748, 2112, 2246, 2591
SspI	AATATT	3 cut(s)	786, 1339, 1860
StyD4I	CCNGG	5 cut(s)	237, 552, 1387, 2601, 2700
StyI	CCWWGG	3 cut(s)	125, 229, 1266
TaaI	ACNGT	5 cut(s)	1213, 1380, 1958, 2205, 2323
TaiI	ACGT	1 cut(s)	1102
TaqI	TCGA	7 cut(s)	346, 489, 865, 949, 1874, 2282, 2416
TatI	WGTACW	3 cut(s)	2032, 2200, 2297
TauI	GCSGC	2 cut(s)	445, 689
TfiI	GAWTC	9 cut(s)	121, 147, 176, 509, 521, 695, 1355, 2216, 2353
TscAI	CASTG	3 cut(s)	226, 523, 2388
TseFI	GTSAC	2 cut(s)	1868, 2450
TseI	GCWGC	5 cut(s)	24, 1106, 1190, 2618, 2672
Tsp45I	GTSAC	2 cut(s)	1868, 2450
TspGWI	ACGGA	1 cut(s)	2624
TspRI	CASTG	3 cut(s)	226, 523, 2388
Tth111I	GACNNNGTC	1 cut(s)	931
Van91I	CCANNNNNTGG	1 cut(s)	303
VpaK11BI	GGWCC	3 cut(s)	836, 927, 2070
VspI	ATTAAT	1 cut(s)	701
XapI	RAATTY	7 cut(s)	613, 1064, 1154, 1774, 1820, 2256, 2677
XbaI	TCTAGA	2 cut(s)	86, 140
XceI	RCATGY	2 cut(s)	667, 2409
XhoI	CTCGAG	1 cut(s)	2415
XmaJI	CCTAGG	1 cut(s)	229
XmiI	GTMKAC	3 cut(s)	809, 1672, 2535
XmnI	GAANNNNTTC	2 cut(s)	1467, 1695
ZrmI	AGTACT	1 cut(s)	2034
Zsp2I	ATGCAT	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.