FvH4_3g22390

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007352
Physical Location & Seq
Forward (+)
15421893 .. 15425162
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22390.t1

Sequence Viewer

Length: 753 bp
ATGAGGAGGAGACCTGGTATTGGTGGTTTACAGAATGCTGCTGCTGCTAGGGACCAATATCGGGCGCTCGGCCAGAATGTGGCCCAGATTAGAACGGATCTCATGAAGGAGCAGCTTGCCACTTTTAAAAGCCAGTTAGAAGATTTTGCTCGCAAACATAAGAATGACATTCGCAAGAATCCTGCTTTTAGATCACAATTCCATGATATGTGCACTAAGGTTGGAGTAGACCCCTTGGCCTCCAATAAGGGTTTCTGGGCAGAGCTTTTGGGGATTGGTGACTTCTACTACGAACTTGGAGTGCAAATTGTTGATATATGCTTAGCAACAAGACCCCACAACGGAGGCTTGATCAACCTGCAGGAACTCTGCAATTTGCTTCGTCAGAGACGAAAGAATGACCGTGAAGCTGTTTCCGAGGATGATTGCCTGCGTGCTATAAGTAAGCTGAAGGTATTGGGTAATGGTTTTGAGGTTATTTCTGTTGGAAAGAGGAAGCTTGTCCGTTCTGTTCCAACTGAGTTGAACAAAGACCATAATGAAATTTTAGAGCTTGCCCAGTCTCAAGGCTTTGTGACTGTTGATGAGGTAGAGAGACGGCTATCTTGGACCAGTGGCCGTGCAATTGATGCCCTTGACACTTTACTAGATGAGGGTCTTGCAATGATTGATGATGGCCACAAAGATGGTACACGCCGATATTGGTTCCCATGTGTATCTTCCATCTCTACCCTTGTTGGAGCCGACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
EAP30

Protein Analysis

251

Amino Acids

28.07

Weight (kDa)

8.25

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAP30 PF04157 6 - 225 7e-57 EAP30/Vps36 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 366
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 1 cut(s) 228
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 3 cut(s) 70, 616, 676
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 470
AfaI GTAC 1 cut(s) 691
AfiI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 13
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 6 cut(s) 115, 265, 410, 448, 499, 553
AluI AGCT 6 cut(s) 115, 265, 410, 448, 499, 553
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 4 cut(s) 4, 382, 567, 589
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 105
AoxI GGCC 5 cut(s) 70, 81, 237, 616, 676
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 4 cut(s) 38, 41, 44, 112
ApoI RAATTY 1 cut(s) 543
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 3 cut(s) 52, 82, 609
AsuHPI GGTGA 1 cut(s) 290
AvaII GGWCC 2 cut(s) 52, 609
BaeGI GKGCMC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 4 cut(s) 25, 28, 31, 124
BccI CCATC 3 cut(s) 668, 680, 731
BceAI ACGGC 2 cut(s) 603, 614
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 4 cut(s) 4, 382, 567, 589
BfaI CTAG 2 cut(s) 48, 647
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 4 cut(s) 39, 42, 45, 113
BlpI GCTNAGC 1 cut(s) 322
BlsI GCNGC 4 cut(s) 40, 43, 46, 114
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 52, 609
BmgT120I GGNCC 3 cut(s) 52, 82, 609
BmiI GGNNCC 3 cut(s) 53, 707, 742
BmrFI CCNGG 1 cut(s) 15
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 1 cut(s) 619
BmuI ACTGGG 1 cut(s) 553
Bpu1102I GCTNAGC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 549
BsaI GGTCTC 1 cut(s) 4
BsaJI CCNNGG 2 cut(s) 234, 417
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
Bse1I ACTGG 3 cut(s) 133, 559, 612
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 234, 417
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 3 cut(s) 133, 559, 612
BseRI GAGGAG 2 cut(s) 19, 22
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 4 cut(s) 25, 28, 31, 124
BshFI GGCC 5 cut(s) 72, 83, 239, 618, 678
BsiHKAI GWGCWC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 4 cut(s) 20, 61, 79, 341
BsmAI GTCTC 4 cut(s) 4, 382, 567, 589
BsmBI CGTCTC 2 cut(s) 382, 589
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 5 cut(s) 72, 83, 239, 618, 678
Bso31I GGTCTC 1 cut(s) 4
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 3 cut(s) 97, 191, 351
Bsp1720I GCTNAGC 1 cut(s) 322
BspANI GGCC 5 cut(s) 72, 83, 239, 618, 678
BspCNI CTCAG 1 cut(s) 511
BspHI TCATGA 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 53, 707, 742
BspMAI CTGCAG 1 cut(s) 363
BspMI ACCTGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 105
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 3 cut(s) 133, 559, 612
BssECI CCNNGG 2 cut(s) 234, 417
BssMI GATC 3 cut(s) 97, 191, 351
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 2 cut(s) 404, 580
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
BstDEI CTNAG 3 cut(s) 216, 322, 519
BstF5I GGATG 1 cut(s) 427
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 3 cut(s) 100, 194, 354
BstMAI GTCTC 4 cut(s) 4, 382, 567, 589
BstMBI GATC 3 cut(s) 97, 191, 351
BstMWI GCNNNNNNNGC 2 cut(s) 44, 629
BstNI CCWGG 1 cut(s) 15
BstSCI CCNGG 1 cut(s) 13
BstSFI CTRYAG 1 cut(s) 359
BstSLI GKGCMC 1 cut(s) 215
BstV1I GCAGC 4 cut(s) 25, 28, 31, 124
BstX2I RGATCY 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 1 cut(s) 97
BsuRI GGCC 5 cut(s) 72, 83, 239, 618, 678
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 5 cut(s) 117, 151, 431, 435, 555
CciI TCATGA 1 cut(s) 102
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 3 cut(s) 52, 82, 609
CsiI ACCWGGT 1 cut(s) 13
Csp6I GTAC 1 cut(s) 690
CviAII CATG 3 cut(s) 103, 203, 711
CviQI GTAC 1 cut(s) 690
DdeI CTNAG 3 cut(s) 216, 322, 519
DpnI GATC 3 cut(s) 99, 193, 353
DpnII GATC 3 cut(s) 97, 191, 351
DraI TTTAAA 1 cut(s) 127
EaeI YGGCCR 3 cut(s) 70, 616, 676
Eco130I CCWWGG 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 4
Eco47I GGWCC 2 cut(s) 52, 609
Eco57I CTGAAG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 2 cut(s) 382, 589
FaeI CATG 3 cut(s) 106, 206, 714
FaiI YATR 9 cut(s) 104, 159, 204, 209, 317, 319, 440, 537, 712
FaqI GGGAC 1 cut(s) 65
FatI CATG 3 cut(s) 102, 202, 710
FbaI TGATCA 1 cut(s) 351
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 39, 42, 45, 113
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 4 cut(s) 39, 42, 45, 113
FspBI CTAG 2 cut(s) 48, 647
GlaI GCGC 1 cut(s) 66
GluI GCNGC 4 cut(s) 39, 42, 45, 113
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 5 cut(s) 72, 83, 239, 618, 678
HhaI GCGC 1 cut(s) 67
Hin1II CATG 3 cut(s) 106, 206, 714
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 1 cut(s) 178
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 4 cut(s) 29, 213, 229, 692
Hpy188I TCNGA 2 cut(s) 387, 418
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 4 cut(s) 29, 213, 229, 692
HpyAV CCTTC 2 cut(s) 100, 445
HpyCH4III ACNGT 2 cut(s) 404, 580
HpyCH4V TGCA 6 cut(s) 213, 304, 361, 372, 623, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 629
HpyF3I CTNAG 3 cut(s) 216, 322, 519
Hsp92II CATG 3 cut(s) 106, 206, 714
HspAI GCGC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 351
Kzo9I GATC 3 cut(s) 97, 191, 351
LmnI GCTCC 2 cut(s) 109, 740
Lsp1109I GCAGC 4 cut(s) 25, 28, 31, 124
LweI GCATC 1 cut(s) 619
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 2 cut(s) 48, 647
MaeIII GTNAC 2 cut(s) 278, 574
MalI GATC 3 cut(s) 99, 193, 353
MboI GATC 3 cut(s) 97, 191, 351
MboII GAAGA 2 cut(s) 152, 711
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 5 cut(s) 197, 306, 373, 543, 624
MluNI TGGCCA 1 cut(s) 678
MmeI TCCRAC 4 cut(s) 202, 466, 539, 718
MnlI CCTC 7 cut(s) 250, 338, 412, 466, 486, 580, 646
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 2 cut(s) 162, 684
Msp20I TGGCCA 1 cut(s) 678
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 1 cut(s) 15
MwoI GCNNNNNNNGC 2 cut(s) 44, 629
NdeII GATC 3 cut(s) 97, 191, 351
NlaIII CATG 3 cut(s) 106, 206, 714
NlaIV GGNNCC 3 cut(s) 53, 707, 742
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 2 cut(s) 278, 574
PagI TCATGA 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 388
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 4 cut(s) 40, 43, 46, 114
Psp6I CCWGG 1 cut(s) 13
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 3 cut(s) 53, 707, 742
PspPI GGNCC 3 cut(s) 52, 82, 609
PstI CTGCAG 1 cut(s) 363
PsuI RGATCY 1 cut(s) 97
RsaI GTAC 1 cut(s) 691
RsaNI GTAC 1 cut(s) 690
RseI CAYNNNNRTG 2 cut(s) 162, 684
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 4 cut(s) 39, 42, 45, 113
Sau3AI GATC 3 cut(s) 97, 191, 351
Sau96I GGNCC 3 cut(s) 52, 82, 609
SbfI CCTGCAGG 1 cut(s) 363
ScrFI CCNGG 1 cut(s) 15
SdaI CCTGCAGG 1 cut(s) 363
SduI GDGCHC 1 cut(s) 215
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 1 cut(s) 359
SinI GGWCC 2 cut(s) 52, 609
SmiMI CAYNNNNRTG 2 cut(s) 162, 684
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
Sse8387I CCTGCAGG 1 cut(s) 363
Sse9I AATT 5 cut(s) 197, 306, 373, 543, 624
SspMI CTAG 2 cut(s) 48, 647
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 2 cut(s) 404, 580
TasI AATT 5 cut(s) 197, 306, 373, 543, 624
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 2 cut(s) 278, 574
TseI GCWGC 4 cut(s) 38, 41, 44, 112
Tsp45I GTSAC 2 cut(s) 278, 574
TspDTI ATGAA 2 cut(s) 119, 555
TspGWI ACGGA 3 cut(s) 110, 357, 494
TspRI CASTG 1 cut(s) 619
Van91I CCANNNNNTGG 1 cut(s) 79
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 52, 609
XapI RAATTY 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 48, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.